LOCUS       DASJPH010000001        68526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJPH010000001 DASJPH010000000
VERSION     DASJPH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.001"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..971
                     /locus_tag="U3995_000001"
     CDS             117..971
                     /locus_tag="U3995_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1795357.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="U3995_000002"
     CDS             972..2309
                     /locus_tag="U3995_000002"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1795358.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="U3995_000003"
     CDS             2314..2538
                     /locus_tag="U3995_000003"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1795359.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="U3995_000004"
     CDS             2540..3430
                     /locus_tag="U3995_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1795360.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="U3995_000005"
     CDS             3439..4257
                     /locus_tag="U3995_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1795361.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="U3995_000006"
     CDS             4364..4813
                     /locus_tag="U3995_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1795362.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="U3995_000007"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="U3995_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1795363.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="U3995_000008"
     CDS             complement(6568..6684)
                     /locus_tag="U3995_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1795364.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="U3995_000009"
     CDS             complement(6757..7713)
                     /locus_tag="U3995_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1795365.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="U3995_000010"
     CDS             7884..8252
                     /locus_tag="U3995_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1795366.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="U3995_000011"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="U3995_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1795367.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="U3995_000012"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="U3995_000012"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1795368.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="U3995_000013"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="U3995_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1795369.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="U3995_000014"
     CDS             10240..12432
                     /locus_tag="U3995_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1795370.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="U3995_000015"
     CDS             12458..13420
                     /locus_tag="U3995_000015"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1795371.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="U3995_000016"
     CDS             13434..14795
                     /locus_tag="U3995_000016"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1795372.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="U3995_000017"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="U3995_000017"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1795373.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="U3995_000018"
     CDS             15924..17144
                     /locus_tag="U3995_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1795374.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="U3995_000019"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="U3995_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1795375.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="U3995_000020"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="U3995_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1795376.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="U3995_000021"
     CDS             19906..20583
                     /locus_tag="U3995_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1795377.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="U3995_000022"
     CDS             20597..21196
                     /locus_tag="U3995_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1795378.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="U3995_000023"
     CDS             21211..21498
                     /locus_tag="U3995_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1795379.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="U3995_000024"
     CDS             21547..22329
                     /locus_tag="U3995_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1795380.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="U3995_000025"
     CDS             22350..23057
                     /locus_tag="U3995_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1795381.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="U3995_000026"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="U3995_000026"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1795382.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="U3995_000027"
     CDS             complement(26166..27692)
                     /locus_tag="U3995_000027"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1795383.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="U3995_000028"
     CDS             complement(27707..28354)
                     /locus_tag="U3995_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1795384.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="U3995_000029"
     CDS             complement(28538..28867)
                     /locus_tag="U3995_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1795385.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="U3995_000030"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="U3995_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1795386.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="U3995_000031"
     CDS             29788..30801
                     /locus_tag="U3995_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1795387.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="U3995_000032"
     CDS             30801..32078
                     /locus_tag="U3995_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1795388.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="U3995_000033"
     CDS             32141..34843
                     /locus_tag="U3995_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1795389.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="U3995_000034"
     CDS             34995..35312
                     /locus_tag="U3995_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1795390.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="U3995_000035"
     CDS             35342..35662
                     /locus_tag="U3995_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1795391.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35770..37230
                     /locus_tag="U3995_000036"
     CDS             35770..37230
                     /locus_tag="U3995_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1795392.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37298..37519
                     /locus_tag="U3995_000037"
     CDS             37298..37519
                     /locus_tag="U3995_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795393.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37550..37732
                     /locus_tag="U3995_000038"
     CDS             37550..37732
                     /locus_tag="U3995_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1795394.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37732..38145
                     /locus_tag="U3995_000039"
     CDS             37732..38145
                     /locus_tag="U3995_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1795395.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38268..39449
                     /locus_tag="U3995_000040"
     CDS             38268..39449
                     /locus_tag="U3995_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1795396.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39520..39684)
                     /locus_tag="U3995_000041"
     CDS             complement(39520..39684)
                     /locus_tag="U3995_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795397.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39838..39911
                     /locus_tag="U3995_000042"
     tRNA            39838..39911
                     /locus_tag="U3995_000042"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39872..39874,aa:Arg,seq:cct)
     gene            complement(39980..41119)
                     /locus_tag="U3995_000043"
     CDS             complement(39980..41119)
                     /locus_tag="U3995_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1795398.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41418..42053)
                     /locus_tag="U3995_000044"
     CDS             complement(41418..42053)
                     /locus_tag="U3995_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795399.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42166..42801)
                     /locus_tag="U3995_000045"
     CDS             complement(42166..42801)
                     /locus_tag="U3995_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN1795400.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42835..43296)
                     /locus_tag="U3995_000046"
     CDS             complement(42835..43296)
                     /locus_tag="U3995_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN1795401.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43426..43857)
                     /locus_tag="U3995_000047"
     CDS             complement(43426..43857)
                     /locus_tag="U3995_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1795402.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43875..44195)
                     /locus_tag="U3995_000048"
     CDS             complement(43875..44195)
                     /locus_tag="U3995_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1795403.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44494..45270
                     /locus_tag="U3995_000049"
     CDS             44494..45270
                     /locus_tag="U3995_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1795404.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45285..45488
                     /locus_tag="U3995_000050"
     CDS             45285..45488
                     /locus_tag="U3995_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1795405.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45504..45842
                     /locus_tag="U3995_000051"
     CDS             45504..45842
                     /locus_tag="U3995_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN1795406.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45829..46008
                     /locus_tag="U3995_000052"
     CDS             45829..46008
                     /locus_tag="U3995_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795407.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46051..46521)
                     /locus_tag="U3995_000053"
     CDS             complement(46051..46521)
                     /locus_tag="U3995_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795408.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46608..47306
                     /locus_tag="U3995_000054"
     CDS             46608..47306
                     /locus_tag="U3995_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1795409.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47484..47825
                     /locus_tag="U3995_000055"
     CDS             47484..47825
                     /locus_tag="U3995_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795410.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47818..48489
                     /locus_tag="U3995_000056"
     CDS             47818..48489
                     /locus_tag="U3995_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1795411.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48495..49187
                     /locus_tag="U3995_000057"
     CDS             48495..49187
                     /locus_tag="U3995_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795412.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            49184..49933
                     /locus_tag="U3995_000058"
     CDS             49184..49933
                     /locus_tag="U3995_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1795413.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49945..50214
                     /locus_tag="U3995_000059"
     CDS             49945..50214
                     /locus_tag="U3995_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795414.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            50220..50384
                     /locus_tag="U3995_000060"
     CDS             50220..50384
                     /locus_tag="U3995_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795415.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            50377..50676
                     /locus_tag="U3995_000061"
     CDS             50377..50676
                     /locus_tag="U3995_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795416.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            50676..50978
                     /locus_tag="U3995_000062"
     CDS             50676..50978
                     /locus_tag="U3995_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1795417.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            51018..51383
                     /locus_tag="U3995_000063"
     CDS             51018..51383
                     /locus_tag="U3995_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795418.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            51873..52013
                     /locus_tag="U3995_000064"
     CDS             51873..52013
                     /locus_tag="U3995_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795419.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            52010..52192
                     /locus_tag="U3995_000065"
     CDS             52010..52192
                     /locus_tag="U3995_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795420.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            52205..52399
                     /locus_tag="U3995_000066"
     CDS             52205..52399
                     /locus_tag="U3995_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795421.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(52432..52644)
                     /locus_tag="U3995_000067"
     CDS             complement(52432..52644)
                     /locus_tag="U3995_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795422.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            52769..52972
                     /locus_tag="U3995_000068"
     CDS             52769..52972
                     /locus_tag="U3995_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795423.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            52969..53241
                     /locus_tag="U3995_000069"
     CDS             52969..53241
                     /locus_tag="U3995_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN1795424.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            53242..53427
                     /locus_tag="U3995_000070"
     CDS             53242..53427
                     /locus_tag="U3995_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795425.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            53414..53761
                     /locus_tag="U3995_000071"
     CDS             53414..53761
                     /locus_tag="U3995_000071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795426.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            53721..54197
                     /locus_tag="U3995_000072"
     CDS             53721..54197
                     /locus_tag="U3995_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN1795427.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(54343..55071)
                     /locus_tag="U3995_000073"
     CDS             complement(54343..55071)
                     /locus_tag="U3995_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN1795428.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            55119..55397
                     /locus_tag="U3995_000074"
     CDS             55119..55397
                     /locus_tag="U3995_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795429.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            55399..55785
                     /locus_tag="U3995_000075"
     CDS             55399..55785
                     /locus_tag="U3995_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795430.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            55782..56162
                     /locus_tag="U3995_000076"
     CDS             55782..56162
                     /locus_tag="U3995_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1795431.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            56274..56726
                     /locus_tag="U3995_000077"
     CDS             56274..56726
                     /locus_tag="U3995_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN1795432.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            56723..58450
                     /locus_tag="U3995_000078"
     CDS             56723..58450
                     /locus_tag="U3995_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1795433.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            58514..59701
                     /locus_tag="U3995_000079"
     CDS             58514..59701
                     /locus_tag="U3995_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1795434.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            59673..60242
                     /locus_tag="U3995_000080"
     CDS             59673..60242
                     /locus_tag="U3995_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN1795435.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            60255..61619
                     /locus_tag="U3995_000081"
     CDS             60255..61619
                     /locus_tag="U3995_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1795436.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            61621..61902
                     /locus_tag="U3995_000082"
     CDS             61621..61902
                     /locus_tag="U3995_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1795437.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            61880..62206
                     /locus_tag="U3995_000083"
     CDS             61880..62206
                     /locus_tag="U3995_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1795438.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            62196..62534
                     /locus_tag="U3995_000084"
     CDS             62196..62534
                     /locus_tag="U3995_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795439.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            62524..62889
                     /locus_tag="U3995_000085"
     CDS             62524..62889
                     /locus_tag="U3995_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1795440.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            62896..63522
                     /locus_tag="U3995_000086"
     CDS             62896..63522
                     /locus_tag="U3995_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1795441.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            63522..63986
                     /locus_tag="U3995_000087"
     CDS             63522..63986
                     /locus_tag="U3995_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795442.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            64181..66490
                     /locus_tag="U3995_000088"
     CDS             64181..66490
                     /locus_tag="U3995_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1795443.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            66502..67206
                     /locus_tag="U3995_000089"
     CDS             66502..67206
                     /locus_tag="U3995_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1795444.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            67203..>68526
                     /locus_tag="U3995_000090"
     CDS             67203..>68526
                     /locus_tag="U3995_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1795445.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITS"
BASE COUNT        23331 a        11282 c        14268 g        19645 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgatcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt
    35701 gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg
    35761 aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca
    35821 aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc
    35881 gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa
    35941 cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg
    36001 aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg
    36061 atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct
    36121 ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca
    36181 gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt
    36241 ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat
    36301 catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt
    36361 ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca
    36421 gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt
    36481 atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa
    36541 tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca
    36601 gtatttgttc aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg
    36661 ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat
    36721 aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg
    36781 tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc
    36841 ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta
    36901 tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta
    36961 ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga
    37021 tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta
    37081 ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca
    37141 ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa
    37201 atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt
    37261 ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat
    37321 gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac
    37381 atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt
    37441 tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt
    37501 tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa
    37561 cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc
    37621 gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta
    37681 tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc
    37741 acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt
    37801 ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag
    37861 tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa
    37921 aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa
    37981 atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat
    38041 ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg
    38101 agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg
    38161 tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat
    38221 ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag
    38281 taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta
    38341 atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga
    38401 attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc
    38461 cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc
    38521 cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc
    38581 gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct
    38641 atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt
    38701 ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa
    38761 tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact
    38821 ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg
    38881 ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg
    38941 cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc
    39001 acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt
    39061 atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg
    39121 tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc
    39181 tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg
    39241 aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa
    39301 gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc
    39361 aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc
    39421 aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca
    39481 ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt
    39541 cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat
    39601 tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac
    39661 tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc
    39721 tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt
    39781 ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt
    39841 cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc
    39901 agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt
    39961 gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga
    40021 ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag
    40081 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
    40141 gtaaaataga tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta
    40201 attcttttaa ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt
    40261 ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt
    40321 tttgattgat tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc
    40381 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
    40441 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagtccag
    40501 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
    40561 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
    40621 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
    40681 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
    40741 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
    40801 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
    40861 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
    40921 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
    40981 attctaattc gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta
    41041 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
    41101 gtttgactaa tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata
    41161 aagaagccca ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa
    41221 ggatgtgcta tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga
    41281 ggtgtttatt atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt
    41341 atttgttgat tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt
    41401 catattagaa agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat
    41461 attttgtagc ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt
    41521 tctaacagac caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag
    41581 ccagacatgc tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac
    41641 aattgaatct caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc
    41701 catgaagcgt tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt
    41761 ttattttgtg cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt
    41821 tggtccttgt tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat
    41881 gattcgttta aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac
    41941 ccaaaataaa gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca
    42001 actattaatt caagatattc ttttttggtt agtttttcat tgctattatc catagttacc
    42061 tcctattttg tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg
    42121 ttttattatt gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac
    42181 tttactatta atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact
    42241 aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc
    42301 cttcgcaaaa attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc
    42361 atcgtactct ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc
    42421 ttcaaaagta tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc
    42481 tggagtaata gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa
    42541 aaattcttca aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc
    42601 agatgatgag ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa
    42661 acctacggta gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt
    42721 ttttttgata aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt
    42781 aaaaccaaat ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa
    42841 accaagcaat tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa
    42901 caactcttta tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt
    42961 atctaatata gattgttgct tctctatata tttttttaaa ttttccattt cattccaata
    43021 tttttttgta aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact
    43081 ttcgtttccg cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa
    43141 ttgtacggct gatatatttt taaaggggat agttttttct cctttaatac cttgattaac
    43201 taaatttata aatccttttc taataattga aatagaatca tcagttactt taatcaaggt
    43261 ttttcctgga gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata
    43321 tgattttttt tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt
    43381 ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct
    43441 tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag
    43501 cttattctga atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt
    43561 cagttcgttc tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt
    43621 ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga
    43681 ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag
    43741 attttcattc aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc
    43801 catataaact actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca
    43861 aaccatcact ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc
    43921 attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa
    43981 tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg
    44041 aaatagtcag caatccgact taattcatct gaattaggag tattattttt ccactttgct
    44101 agataaccgt tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc
    44161 ttcgccaagt cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt
    44221 tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta
    44281 tactatatct tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg
    44341 taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc
    44401 tcttagtata gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt
    44461 tgtttacgaa tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc
    44521 gaacaaaacg aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac
    44581 gttgcgagtg ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg
    44641 gaagataaga tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc
    44701 aacgaatctg gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt
    44761 aaacgttggg taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact
    44821 ccagaaaaag tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa
    44881 ctaaaagaag aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct
    44941 aaaatctcct atttagatag catattatct tctacagatt cagtaacaat tagtcagatt
    45001 gcagcagatt atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt
    45061 cagaaaaaag tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca
    45121 aaatctcata caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc
    45181 aaatggacac agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac
    45241 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    45301 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa
    45361 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    45421 acggtaaaaa gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg
    45481 aattgtaaag ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag
    45541 gctatgtaat tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta
    45601 gatggatgac gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt
    45661 ctagagtttt aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg
    45721 gatttgtcta ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac
    45781 ttgaattttt agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt
    45841 aatagagcgc tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    45901 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
    45961 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
    46021 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
    46081 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
    46141 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
    46201 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
    46261 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
    46321 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
    46381 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
    46441 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
    46501 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
    46561 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
    46621 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
    46681 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
    46741 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
    46801 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
    46861 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
    46921 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
    46981 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    47041 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
    47101 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    47161 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    47221 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
    47281 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
    47341 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
    47401 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
    47461 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
    47521 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
    47581 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
    47641 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
    47701 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
    47761 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
    47821 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    47881 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    47941 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
    48001 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
    48061 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt
    48121 ttagattatc gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg
    48181 aagcgatgct ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg
    48241 gaagatattc caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt
    48301 ctagaagctt tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt
    48361 aatgagcaga cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta
    48421 agctatgagc aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat
    48481 aagaagtgat atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt
    48541 tcaaaggaaa agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga
    48601 cgatgtatga cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac
    48661 gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata
    48721 caggcatgcc aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag
    48781 ggcgcaaaat aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag
    48841 caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga
    48901 ttttacctgc gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca
    48961 tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc
    49021 gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag
    49081 agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat
    49141 taaaccagaa aacaatcaaa aagttaaata ttggagggga aaaatgacag aacatcgaag
    49201 ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa
    49261 gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa
    49321 tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga
    49381 tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat
    49441 aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat
    49501 tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga
    49561 atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata
    49621 cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt
    49681 ccctaatgat taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa
    49741 gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc
    49801 taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc
    49861 aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa
    49921 agcaccattc taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata
    49981 gcgaattata aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa
    50041 gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaaact cagatactat
    50101 ttcgcactct atatcgatgg aacgcaatta agaaatttag catataccaa attactattc
    50161 attgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga
    50221 tggacctaaa gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa
    50281 gtgtgtcgta cgaagacaag tggctcaaca catttcatgg cggacgtgag agcgcacttg
    50341 atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag
    50401 cgaattatcg aattgactta ttcagaagat tggcaggaag ataaaaaaat agttgcagaa
    50461 gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag
    50521 attgcaatct ggcatggtga tcgaattcta gtaacgggta ccgctaaaca gttatctgaa
    50581 attactggtc tgagcaaaaa tattatttgg gatagagcta ggagcttatg gattgattca
    50641 aaaggaagac agtttaggta tgtggaggag aaataatgga cgaactaatc gcgaaagtag
    50701 agcagtgggc aaaagataag ggattggatc aagcggattt cagcaagcaa atgttaaaaa
    50761 cggtcgaaga aatcggggaa gttgccgctt ctctagctag aaaagatgaa catggtttaa
    50821 gagacggaat tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg
    50881 atttatacga gtgcttggat tttgcttatg acgaaatcaa aggtcggact ggaaagatgg
    50941 tcaatggtgt tttcgttaag tccagtgacc tgaaataatt ccattaatgg aagaaataac
    51001 tgaataggag gatagacatg attgatgcaa aacaagtgca aaaacagaaa gatggaatgt
    51061 tgatgtttga agcatatgtt ctaccatttc ttaatcaatt tgaagttttg gaatgctctg
    51121 ccagcggaga agaattggag tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc
    51181 taaacgaatt tctttgcact attaactgtt gggatatgat agctccagga tttttatgtc
    51241 cagctatggg agaatttttg gaatattgtc gtcttgaaga tgcaggcact ttggatttag
    51301 cttatctggt atacaactac ctaaacatca acacagatca tttgtggttt ggcactgccg
    51361 aaagaaaatg ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag
    51421 tggaattgcc gccattgctg taaggttctt tacgcgactc aaggtcacag aaatataaat
    51481 gcaaatctct tttgaagtga tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga
    51541 ataaatgaga tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc caaagaaccg
    51601 aatgagctaa ggacatcatg tgtaaacaca taatatagct ctcctttcac ggctaatcat
    51661 aacatgaaaa atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat
    51721 tatcccggca gtaacagatt ctgcaattaa actccgaagc tgatgtatca gtatcaactg
    51781 tagaatatta tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca
    51841 acggtcttat ctgtttagat gaaggcgata gtatgaatga aattgctgca gaccatgaat
    51901 tatcagaaac tgctttaatt gatcaaatcg aattagctag acaatacttt gttccatatg
    51961 cagatacctg gtccaagcat aagaatgaca tcatttttcc agaggaacaa tgagagcaac
    52021 tgatccagta attatccttg aggaagccaa atttatttgg actcacgaag agatagagca
    52081 agcacgcttg ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa taatgggtca
    52141 aaagattctt gatgtcggat tgcttttgct ccatctagca gaaaaaattt gatttgaggt
    52201 gaaaatgatg attcaacttg caggcatcca aacagggaaa atttattttt ctggagaaag
    52261 caaaagcgaa gccaaccaat ggctattgaa aacttacacg aacaataaga agctaaggaa
    52321 aaaatatcct gatttgttgc taaaggatga tcagattatg ccggaaccga tgattttgac
    52381 ccgtaaggag ggaagttgaa actaaaaaac cagccgtgtg ggctggagtt gctatttctt
    52441 tttaataatg agtttttcta aatttaattc attctcacga gtttgcgtcc aataatcaga
    52501 ataatatttt aattgtttaa gagtaggaat gtcgtcttcg gtaaaattag aaaataattc
    52561 tggagaatta tcttttttta attgttctaa tcctgattct ttaaaacgtt gttcaacgag
    52621 ttttactaaa ttgttgatat tcatgataaa tcaccttttt aaatttaatt tcgatttgat
    52681 taatcgatac aagtattata tcaaataaaa aagctgattt agggggtctt acaaaaacac
    52741 taaaattttt tcaaaaagag gaggaacggt ggtgaaagtc agagaacaat tcaagatagt
    52801 gagcgacgaa ggtaatgttt acaaaataaa gatcgttaat attaatgaat atcgagaagc
    52861 agatcacaaa tatttggttg acttatacat gaacgataaa tatttgggag aaggattttg
    52921 tggagattca ctttttgatg atgagcgaat tttgaaggag gaaacagaat gatccacgaa
    52981 ctaaaaatac taccagaata ttttgaagca gtgacgagcg gacgcaaaca attcgagatc
    53041 cgcaagaatg atcgtgactt caaagttggt gatcagttaa tactacgtga atataaaaaa
    53101 tacattaaac atgaattacc tggactaacg agaccaacat ttagttacac tggtgactca
    53161 tacaaagcag agatcactta tattactgat tacatgcaga agtccggata tgtagtgttg
    53221 gggattaaag gggtgaatta aatgaaagta tacatcttag ctatcacaga gggcacatgg
    53281 atgttcccag tcggttccgg aaaaatctat aaatcaaaaa cagctgctta taaagcattt
    53341 gaaaaataca aaaaagaaaa cggtggcggc actaacgcaa agattctagt ggctgataac
    53401 tggcatgaag aaggtgaacg taattgattt acctgttcat tttcgtggta ctaactattt
    53461 ttatgctagt aatcttattc aaaaaaagat gagtaaagac gaacgaaaaa ttctggccaa
    53521 cttttattta gtattaaaaa tcgaacaaaa tgtgttttcg gaaaggagaa ttgtatggga
    53581 aaaacaaaat caaagattaa gaagaaaaaa cgacgtttgg aacaaaaggc aatccagaat
    53641 ggaacggcta agaaaaaata aaaaagctac ccctttcgag atagcagcgc aatactattc
    53701 taccataagg ggtggcgttt gtgagatttc attggttaaa agattaccaa gaactagatg
    53761 agcagattct ttacttaaag tggaatctta ataaaagtaa gcttgaattg aatcgatggg
    53821 tctgtggtga tttagcaaac gtccgcatcg aaaagaattc gagaacttct tctttagaag
    53881 agaatatcca aaagatagaa aatgaattgg aattgttgat cgagcaaaaa gaagaaatgt
    53941 tattgctgat agatagcttt tctggtatcg ataatcaaat tgttaagatg aaatatgttg
    54001 atcaaatgag cttagaagat attgctgaat ctgttgggta tagctcatcg tatgtcagac
    54061 agcgacatgc agaaattaga aagacattga actttttaga tgagtatgaa cacagacaag
    54121 ctgaccgatt gaaaaaagaa aacgaaattg atttttacaa tagcgagaag tacaaagaac
    54181 aactctcttt attctaaaat tacactgttc actaaatgtt cggtcattgt agctatgtaa
    54241 acattgttag cagcatgata ttctattagt gtcaaaaaaa tatgaaagag ccaagatatc
    54301 ccaaccgttt tattaattgg tatctgtggc tcttttattc ttttaattag tagttggcat
    54361 aaagttattt ccatttataa attgatcagc taaatcagca tcataatcag ccgcgataaa
    54421 ttgcgctgct gatttaataa atgcatccat atcatgaaca tctttattag tatgtctacg
    54481 aacatagtgc gtttcatcat taccaatcca agtagcagct ttggctaatc tttggagttt
    54541 aggaaaatca ttcaaataat tttcaattgt tttacctaat aacatttttt ttattttatc
    54601 ttcatcctct gaatttttag atattgcata atctttaatt agaaattcta gagcttttct
    54661 ataaccaact ccagcaattt gttccaattg ctcagattta gcttgtgtgg cttgagtata
    54721 aatatcaaca aatgtaggag aaactttttc aatattttct ggtaaatcta ttttaatcgg
    54781 cggacggtaa taatctttaa ttaaaatatt ttcagaatat ccttcatctt cattaaacgt
    54841 atttataaat tgtaaactat aaaatttgtt gcactcatca tttatacact gtactaatac
    54901 gccaaagatt ctatcattat cgaacgcact atatgatgaa accccaaaat acacatacgg
    54961 agtcattata tttccgcaat gcggacaact atttggcaaa taaattgtct ctgtattttg
    55021 acgatcacga tacttaatat taacttcttg aagcattaaa acacctcgca tcttttttat
    55081 aaatcatacc acaaaatatc tgataggaga gtaaacaaat gaatgatttt catgaagcgg
    55141 tacttagtat tgaagtagaa tcaagtttgg ctaaagttta taagaaagca attgaagcag
    55201 agaatagtcc gtacagagaa aattggaatg gtaatcatgc acatgtgcaa gtggagaatg
    55261 atgattacag aactggaatg aatacgttag ttatttcttt attgtcacac actctaccca
    55321 atctgcagga aacgattgaa tggtatgagc gaatgggcgc aaaagtaatc cgtacaaatt
    55381 acaaaggaga gaattaaaat ggcaatgatc aaaattagaa catccattag tggaacagag
    55441 tattgggatt cagaaaagaa aaggacagta gttgttccga aagatcaaga accaaatttt
    55501 gaagtaaaag aagaaaaagg aattttgagt gatggcaaat catttgttgc tgtcaatggc
    55561 gaactcatag ctcatagcag tgaaattctt gatagcgatg gaaacactgc tgctaatttt
    55621 gatggagacg aagctactaa tgatcagtcc actgaagata cggatgaact ggacaatatg
    55681 actgcaaaag aattgcgtgc atatgctaag aaacatggta ttgatattcc tggtgctatc
    55741 cgtgcaaaag gcgatatcat aaatcttatt cgtgaagtag aatgaaatac tgtcagtttg
    55801 acggatgcac gaacaaaata gcaaagggta tctactgtac tgaacacaag agatcaagca
    55861 gatcacgcaa gaagaagcag caagcaaagt ctgtttatca tcatgagaac aaaccattct
    55921 atcgaacgca agcatggaaa gatatgcgtc aatttattta tgaaagagaa ggtggtcatt
    55981 gtcagcggtg tggtcagttc atatttggaa agagagcaca cgtccatcac attgtaccaa
    56041 tcaaagacaa cgaactgctt aagcttgatc caaacaatct catgctatta tgttcaaaat
    56101 gtcatccaat tgttgaaaac gaaacggaag acaaaaaagt ttttccttcg tatttcaatt
    56161 gaagcccccc tattcatttt caaaattttt tcgcgtgggg agatagggta gcggggagtc
    56221 acgcgcatcg ttaggtcaaa tttttcaaaa aacaaagggg ggtgtataca aaaatgacga
    56281 ctaaagcgca acgtaaagcg attattgatg aaaaagtaag tgctgaaaaa gctcgtatct
    56341 tagaaataat gaatttgtct gatttgtaca ccatcactct tgatccatta atcgaatcat
    56401 acttggatat ttttgaaatt taccaacaca aatatttatt gtggaaggaa aaaggatttc
    56461 cggagacgca aaaattcacg aataaatcag gagctactaa tcaatcaaaa catccattgg
    56521 cacaacaagt agaaacttgg gcagataaga agatgaaagc tctagattta ttaggattaa
    56581 caaacaaagc aaaaactggt agactaattg ctggaggatc gaccgcaaga aaagatgaag
    56641 aaattacacg cccagaagta aagccagtag atgaactagc agcgcacaga aataaatggc
    56701 gtaagaaggc aggtgctgaa aaatgattga acctggtgta aattatgctg atttatttgc
    56761 aaaagaagta agaaagaaac ctaagaagta tcctaagacc gttcgtttag caatagatcg
    56821 ctggtatcag tggaaaaaaa gaaaagatat ttggtttgat gtagatcgcg cgaatgaaat
    56881 gatggattgg gtagaatcat ttattgttca cacaaaagga gaaatggtgg gaaaaccgtt
    56941 tcttttagaa ccctgggaaa aatttattta ttcttggatt tatggttggg ttaaagaaaa
    57001 tgaaaaaggc caaattgtac gcgttactcg tgaggcttat gtccagattc caaaaaagaa
    57061 tggtaaaaca ttgattgctg tcggttcatt aggatatgca atgtacggag aaggggcgtt
    57121 atcagttgat tgctatgctt gcgcatctga ttttgctcaa gcgcaatatg ctgccaaacc
    57181 ttttgcagtt acaatcttaa acaatccaat cctacttgaa ggaactaaaa tatttagggg
    57241 gccaaaggga actgtttcta gtattactta tgactatatt catgaagata tggcttactc
    57301 aaataaattt attgttcaaa ccaagaatat tgataatatc gagggatcca atccatattt
    57361 tgtattgaac gatgaattgc ataaacagga gaaaatggaa cagtatgaca attttaagtc
    57421 tgctcagata tctttaccac agccattgat gtttaatatc tcaactgctg ggaaaggttc
    57481 gtcgtctgtt ggaatgcgtg tatatcgcga agcaaaagaa gtgttgaaac gtgatgataa
    57541 tgattcaaac tttgttctaa tctatgagcc aaataaaaat tatgattgga cggatagaaa
    57601 agtctgggaa atgtgcaatc ctaactgggg aatatcagtc gatttatctg ctttagaatc
    57661 ggcatttaaa acagcgcaac ggtcagctca ttccaaagct gaatttctaa cgaaacactt
    57721 ggatgtattt gtgaacggcg cggataattt ctttgaacag gatcaagtag agccatgttt
    57781 agttaccaca caagaacttg gtgatttaag tggcgaacca tgttatatcg gtttagattt
    57841 atcacgtaca cgagatttga cctgtgtatc tttaaacttc ccaacatggg atgaagatgg
    57901 taaagcggtt cttaaagtaa agcaacttta ttttattccg aatgaagatt tagagtttcg
    57961 agaaaaagaa gataatgttc cttacagtga tttggctgaa caaggtttcg ttgaattttg
    58021 cgacggaaaa atgattgatc aagatcagat tttgcagtac atagaggact gcatgaattt
    58081 atatgatatt cagcaagtga attatgatcc agcgatgagt gacaaacttg ttgagaaatt
    58141 agaaaactta ggattggaat gcgttgaagt tgctcaatat cctaaagtat tgaatgctcc
    58201 ttttgacgat gtcgaacggt tgttttatga gaaacgaatt caatttgata atccattatt
    58261 cctttattgc actttgaacg ttgtagcaat tactaacatc aatggacaaa aagcgccaag
    58321 taaacgtcaa tcaaagaaaa agattgatgg gttcgtggcg tttttgtgtg gtcataagga
    58381 aacgatgaat caaatgacag atattgattc agatgagtta gatgattacc taagttccat
    58441 ctatagataa atagaaaggc ggtgaaaaaa attgaaatta cgtgatcggt tatcgaatgc
    58501 agtctattct tttatggaaa aacgtggata tatcgaggat atttttggac actacacacg
    58561 ttatggtcaa agatatgtga cggattcatc tatcatggaa tcttctgatg tttatgaatt
    58621 agttcaagac atatctaatc aggtggcatt ggccacacca attgttattg gtcccgacgg
    58681 caacgaagtc aaagatcatc acttactaaa tattttgaag agtcccaatg attatttgac
    58741 aggattcgaa tttaccaaat tggaaacaaa tactttattg atcaatggtg agacattccc
    58801 attaacggat agggatcagc ttcatttggc gtacggtgta acaactaaaa tcaatgaacg
    58861 acttcaagaa gaatttgaaa tgaatggtca aaaaatacct ggtcaaatga ttcgacacat
    58921 taagaacatc ggaactgatt cattaaaagg tgctggaata attgatcttg caagaaacac
    58981 tctagaaggc gttctgagcg ctgaaaaagt tttgacggac aaatatacta aaggcggttt
    59041 acttgcgttc atgcttaaac tagacgccca catcaatcca aataatagcg cccaaacaaa
    59101 aattgtcaaa gctatattgg atcaactgga aggaacgcaa aatgagagtg atcattctgt
    59161 taagatgatt cctttgggaa aaggatattc catcgagaca ttaaaaagtc ctgttgatga
    59221 cgcggcaatt ttgaattatt taggtgttta caaaaaagac ttaggaaaat ttctaggaat
    59281 caatgttgat acgtatcaat cgctgatgaa gacagatatt gaaaaagcga tgatgtatct
    59341 gcacaacaaa gcaatcaaac caatattgaa aaacaagagc gaacattaca ccgctctttt
    59401 ttttatgcct aattctggct atagagtgga atggaaaatt aatattttgg attttgttcc
    59461 ttactcaaca aaaacaaata ttggctacaa catcgttcgt accgggatta caagtccaga
    59521 taacgtggca gaaatgcttg gttttcctaa acagaatact ccagaaacac aagctatcta
    59581 tatttcaaat gacttatcta ggattggcca gaaaaatgca acagatgatt ccttaccaac
    59641 gaatgatcaa gacttgaaag gaggtgatgg aaatgaagaa gaaggaaatt cgcacgattg
    59701 acatcaccaa tctttcaaca cgttccgatg aagaaactca tacgaggacc attagcggct
    59761 acgctgctgt attcaacagt caaacacttt tatgggatga cttaagcgaa gtgattgttc
    59821 ctggtgcatt ctcaaaggca attagtaatt cggatgttcg ttgtttgttc aatcacgatt
    59881 ggtccaatgt gctaggacgt actaaaagcg gaacccttcg ccttgaagaa gatgaacgtg
    59941 gtttgaaatt cgaagttgat ttaccggaca caacggtagc aagggactta gttaaatcta
    60001 tggaacgagg agacatcaac caatgtagtt ttggttttgt gccgactgaa gaaacgtggg
    60061 actacaattc tgaaccaatg cttcgaacaa tccatgaagt ggaattatac gaggtttcta
    60121 ttgtgccttt gccagcatac gaagatacag aagctgcatt aagaagtcgt gatgaattag
    60181 aaaaaaacgt cgaagaaaga aaaaaattaa tcaaaaaaat taatcaagcg ctagaagcgt
    60241 aggaggaaaa atacatggat aaagaattat tgaaaaaaat gaaggcacgt cgtgagcaac
    60301 gattgactga attacgtgaa aaagttgaat caggagaatt acgcgaagca gatttagaag
    60361 ccgtgaaaga agaaattgac agtgttattg atgaattgaa cggaattaaa gacgaattag
    60421 gcgcagattc tggaactgat gaaacagacg acaatacaga tgatcaatcg aatagtacgg
    60481 attcagacga aagccgttct ggtgaagaca acgatcaaga agaagattca gacagtgaag
    60541 atagttcaga aaatcgttct ggaatgatta ctcaacagca acgagatgga ttacttggat
    60601 caattaagaa cggattggag gcacgtgcaa aaatgaccaa gaaacaaaaa gatcaacaac
    60661 tacgaaaagc atttgctaat tttgtagctg gaaatatttc tgaagcagaa gctcgagctt
    60721 tagggattga agctggcaac ggttcagtta ctgtcccaga agtaattgca tctgaagtta
    60781 ttacttatgc tcaagaagaa aatttacttc gtaaatacgg aacagtggtg cgaacatcag
    60841 gagatgtcaa atatccaatt cttgtgaaga aagcagatgc gaatgtaaac aagaaagagc
    60901 gttcaactga tattgctgaa acagctattc agtttgatga aattttgctt aatcctgccg
    60961 aattcgatgc tttggcaaca gtaactaaaa aattattaaa aatgtctggt gttccagttg
    61021 aagatattgt tgtggaagaa ttgaaaaaag cttatgtgcg taaagaaatc aattatatgt
    61081 tcaatggtga tgacgctgga aatgaaaatc ctggtgcatt agccaaaaag gctgtagcat
    61141 ttaaaaaacc tttagatcta actgcttcag gtgctgggca aaaattatat gatgcattaa
    61201 tcgaatttaa aaatacacca gtgacagaag tgatgaaaaa gggacgcttt attattaatc
    61261 gagctgcttt gactgccatt gaaaaaatga aaacagatga tggatttcct ttgttgcggc
    61321 cattcacaca agcagaaggt ggaattggtt accaattagt tggctatcct gtggattgga
    61381 cagatgcagc agataaaaag ggtgaaccag acacgccagt tttatatttt ggcgattttt
    61441 ctgcatttaa aattcaagaa gttattggtg ccttggaaat tcaaaaactt gttgaaaaat
    61501 tctctggtaa aaatcaaatt ggattccaaa tttacaactt gctagatggt caattggttt
    61561 attctccatt tgaaccggca gtatatcgct acgaaattac aaaaccagtt ggtggttaag
    61621 atggaagagc aaactaaaga attgtcttta gaggaaaaat tcaaatcaca tattcatttt
    61681 gaagagggca tggatgattc tttgctctct ttttatttaa atatggcaaa agattatgtc
    61741 aaaacagcaa ctggtggcca acaagaatat cttattttga tggttgccgg cattgcctat
    61801 gaatatagag tttcagagga tgaactcgac aaagctatga atgccatgac gccatttatc
    61861 gtgcaaggag cgattcaaaa tgccgaagag acagaccaat aatctcagat ggaaagccga
    61921 attgctagac atcaaaacag gaacagacgg aaacgatcgt ccaactacaa tttacgaatt
    61981 taagcgtcta atattctatg aagaactcgg tgtgacttct caagaaaaat atttatcaca
    62041 gcaagccaag acagacgttg tcagacgaat taaagtcaga tgggataaat ccatcacaga
    62101 gaaattaagt gcgctcaaaa ttgattctgt aacgtataac attactcgca tttatacgaa
    62161 tcccgatgca agagaaatgg agttgagttt agcttatgtc gattagcttt gatgaattga
    62221 aaacagcgct gaaatcaaca aagttagcag tgttcagaga caaagccaga ttagggacga
    62281 tgtatccata cattgtgtac tcaaatgtga gtaacagcaa aaaaatggca tccggtaaag
    62341 tctataaaaa attaccttat taccaagtct ctttttttac acttggaaca gaacaggatt
    62401 tagttgttat tgaacaagcg ctgcaaaatg ctggcattcc atattcagat tttacaggta
    62461 tacaaggtga tgagaacgac gataccgtaa ccaactatta cacatatgtg aggtgtgtgg
    62521 aaaatgccaa gtaataagaa tggtttttct gaaatatcgg attatttagg aaacctttca
    62581 aaagttgatc cgaaaaaatt atcgttggaa tctttagaag aagcagctaa cttttatcta
    62641 aagcagttgc ttcctaatat tcctaagtcg ctcttaaaaa agaaacatat gcgcgatcaa
    62701 ataaaagtag tagttgaaaa cgatcaagtg aaagttcaat ttgaagagac ggccttttac
    62761 tggcgctttg ccgaaaatgg cacaacaaat caacgtgccc aacattttgc aagtggtacg
    62821 tatgaacaaa acaaagaaaa aattgaagaa attatgacta gaaaaatact tgatttatgg
    62881 gaagggtgat tttaattggg aaaacaagat actttttatt ttgaaggatt ggacgacatt
    62941 ttaattgcga tgatggcaac gccagattct gttggaactg caccgactta tagtgaagtt
    63001 ttacgattgc caattgccac aaaaatcggt gtcaaaggga atggaacagc attagaaaaa
    63061 tgggcatcca gcaaaatgtt tcgtcgtgtg tcgcgagaaa caaaacatga acttgcgttg
    63121 gatcacgtgg gtattccgat agaagtgatg gatgaaatca aaggattagt tgctaaaagc
    63181 ggagtaacgt ttagcaaaaa taccgcacga gaatttccgt acttcgcctt tggatttatt
    63241 ggaaatattg aaaatggcgg taaaaaagca gtttggtatc ctaatacaca actatccaat
    63301 gtgattgacg aagagtatgc cactgcagag gaagaaacaa aaattgacga tgtaactgca
    63361 aatctggttt ccatcggact gaaatacaat aatgtgatgt atgcaagttt tgattcaaat
    63421 agagacggag cttctctaga attatttaat aaattcattg ctcaacctgt atacgatgaa
    63481 gaacagtgga aaacattggc taaagttgga ggtgcgggtt aatggctcgg ttatctgatt
    63541 atggaattca cgttgaagac ttaaaaaatt ctgctgttgt aattattcaa ggcgcagaat
    63601 tccctatttc atttactatg caaacgatgg aatttatagc agatgtgtat ggtggagatt
    63661 attcgcaatt tgaatctgat atgaacgcca tgctatctaa aaaagaagga caaatttctt
    63721 ctgctaactt atcgcctagt gatctaaaaa tcatgcgtgc cttgatttat gccatgctgc
    63781 gtactggtgg tttagaggaa gatccagaaa ctattttcaa atttttggga atgagtggag
    63841 aggtgttgtc tgcttatagt acctgtatgg aaatttttgc tagccagaca tttcaggtgg
    63901 aagacctaaa aaaatccaag aagccacaag actttcaaaa agcgcaagca aaaagaaaga
    63961 aaaacaaaaa gaatcggaag agataggaac tccttggagt ttttatattt acgttgctct
    64021 cactctattg aattggagtg agagtttctt tttaaagtct acacctaact tgtggctcaa
    64081 atcttattta cagtggttac aacaaaacac cgattttgaa ccacctcaat ctgtaactat
    64141 ggataaatcg ccttggtggt agaaaggagc gctaagataa atggctggta aagaatctga
    64201 tgtcgttctt aattttaaaa cgaatggcga agtcagttat tcgaaaacaa tcaaagaaat
    64261 caacaaagaa atgaacttag ccgctgccga gtacaaaaac caagtgtctg cgatggacaa
    64321 ggatgcaact caaacagaaa aattgcgagc ggctaaacaa aaattagaaa aacaattagg
    64381 attggctgaa caacgatctc aaatgctgag agaggaatat gaaaaatctg tcaaagaaac
    64441 cggtgaatat tctgctgaat cagaaaaact ttataaacaa ttactcaatt ctgaaaccgg
    64501 agaaaataaa cttcgtacag cattagaaca gacgaacgat gcacttaaag aacaaggtga
    64561 cgtttctgtt gatacagcaa aaaaactcca gaaaatcgaa gaaacaggtg agaaagtaaa
    64621 aggcgttggt gaaaaaatgt ctgttggagt aaccgcgcct attgtagcgg caggagcagc
    64681 aggacttgca gcatttggtg aagttgacga ggcacttgat accatcatta caaaaaccgg
    64741 agcaacaggt gatcaagctg atagactttc acagtctttc aaaaacgttg gttcaaatac
    64801 tcatttacct ttacaaacgg ttggggaagc tattggtgag gtaaatacac aatttggatt
    64861 catggataaa aaactggaag attcaaccaa ttatctccta cagtacgctg aaatcaatga
    64921 tacagatgtt tcgcaatcag caatatctgc tcgacaagct attgatgctt atggactaga
    64981 atatgatgat ttgaattctg tccttgatgt aacaacgaaa acatcgcaga atactggtca
    65041 atctgtagac gacttgatgc aaaaagcaat tgatggcgca ccacaaatta aacaattagg
    65101 gttgagcttt ggtgaagggg tcactttact tggacaattt gagcaatctg gtgttgattc
    65161 aagcgcagct ttaagtagtt tatctaaagc aacagtggct tatgcgaaag acggaaaatc
    65221 tttaagtcaa ggactagaag aactacaaga caaggtaaaa aatgcaggtt ctgaaacaga
    65281 agccatcaat gcagcagcag aagtattcgg gactaaaggc ggtcctagaa tggccgatgc
    65341 aattcgtaga ggtactttga acctggaaga tttagcaaaa actgctggag aaagtggggg
    65401 agctgtagga gacacattcg atgctactct cgacccaatt gatcaagccg atcaagctat
    65461 gaacaatgca aaattagcaa tggctgacgt aggtgaagca gtccaagtaa gccttttgcc
    65521 tttttttgaa aaagcaactt ccttattaca ggaattctct aaatggtggg ggtctctaga
    65581 tcaagataca aagaattgga tcataacaat tgctggtata gcggcagcag ttggaccagt
    65641 actaatcgtt cttggttcac ttatgggatc tgttacaaaa atcgttgggg aagttaaaag
    65701 ttttatagga gtttggcaag gacttgctgg tttatttgga atgtcgggag gctggtttgc
    65761 attagcagtt atagctattg gcgcattagt tactggacta atttgggcat ataataacgt
    65821 taaatggttt catgatggag tgaattcttt cttcaaaggt gtttcagatg tagctgttga
    65881 aatatttaat tttttaagtg gttattttgg tggatttttc gatggtgtta tagcaaattt
    65941 caataatttt tataatgctg gaaaacgaat tttcagtggt tttctagatt ttatcactgg
    66001 agtatttaca gggaattggt caaaagcatg gcaaggtcta gttgatattt ttggaggtat
    66061 tttcgatggt attgttgctg tagggaaagc tcctattaat gccatgattg gattgatcaa
    66121 tggatttatt ggtggattga ataatataaa gataccaaaa tgggttcctg gtgtaggtgg
    66181 aaaatcattt tctatttcaa aactgcctta tttagcacaa ggcggtcatc taatcaatgg
    66241 acaagcgatt gttggtgaag ctggacctga gttactgact gccaagaatg gtaaaaccac
    66301 agttactcca ttatcagacg aagaaaaacg caaagggatc agtggaagag tttctggagg
    66361 tagcattgaa cagcatatac acattggcaa agtcgatgca aacaatccat ctgagttaga
    66421 tcgtatgaat cgtaaatttg ctaaagcaaa caaacaggct attcgtgatt tgggaggtgt
    66481 tcctatttga gtcgacaatt tatgaatcca gatgaaccaa attttatttg gaaaaatcgg
    66541 aatgcagtaa ttgatatgaa ttgcatcatt gaggcagagc ttccagatat tatgccgaac
    66601 aagcgatacg aaacttacac gattcagggc cgaagcggag aattgacgga gacgtttgat
    66661 gactatgaac cttttgattt ggaaatagaa gggatcacta ttccacattc gaaactgaga
    66721 gaagtcaaac gatggctcac tggtaaaagc caattaatca cacataatga tccagacaag
    66781 tatctagagg ctatctgcag catggataaa gaagtccctt tcgaaaacga gtggggcttc
    66841 ttttatacat tcgatgttac ttttcgttgt cagccactca aaagaaaaat cggtgaagca
    66901 cctaaaattt ttcattctag tgttatggat atttttgatc atggtgacga aattgcacat
    66961 ccctatctgg aaatagaatc gaacggagga gatataacga tcgctgtcgg tgataaaagc
    67021 ctaacaatac ttaatacgtt agctgcaaca atcgtggttg atactcagct tggaaaagca
    67081 atacaagaag gtttgaattt atttacgcgt ggtgattggc cagtattgca gcctgaatgg
    67141 aatcaggtaa aaatatccgg aaaatttaaa gaagtgcggt tttggaatag gagtgtgtat
    67201 ctgtgacgca agaatttatt tatgcttata aaaaaatgcc agatgatttg agcgtgaacg
    67261 gagcgtcgtt agttgattgg gaagatttgc cagaaatcaa tcgtgtgctg aatggccaat
    67321 atcgttttta tggtaactat tcaagaagcg gacagtatcg ctcttattta aaaaaaggaa
    67381 attttataaa agcgaaggta ccagatggat catggcaata ttttgaaatt tataatgtca
    67441 aaaagaattt gacctccgtt tctgtaacag ctcggcatat tggttttatg gccaataaga
    67501 actttattgt caagtcgttt acggataacg gcaatgggtc tcagatcatg accaatctta
    67561 aaaacagttt ggcatttgat caaaaattta actatctttc gaatgtaggg acaacgcacc
    67621 aatttacagc aagacaagtc gcgccagttg aagcaatcat tggttcaaat aacggtaatc
    67681 aaaatttgac tggcgtgact agcgcggaac ttgatatgga taactatgat ttgaaactgg
    67741 taaaacagat tggatcagat aatggttttc ggattgattt tggcatcaat ttagaagcaa
    67801 tcgaagaaga aattgatgaa gaatcaattg tcaatagttt gtatttggtt ggcggtgttc
    67861 ctgataacga ttatgatgaa gacaaagaac ctatcgaata tggttattta gaaattgatg
    67921 gagtaaccaa cgagaatcga cgtattgcca aacgtgaaaa cggagattgt aaaacagttg
    67981 atgaattgat caaatggggc aaaaccttat ttgacaatga tcgtatccat gagccaaaag
    68041 ctactcatac agtcagtatg gtggcgttgg aacacacact cgaatacggt gacatgtatc
    68101 aagagctagc gtctttacac tttggtgatg tcgctcatgt tagggcgaaa gagctagata
    68161 tcgagataaa agagcgtatg gtcgaataca cttatttccc aacgcttggg aaatataagg
    68221 atattgtttt aggtaatgat ttatcactgt acacttcggc agtaaatact caagcacaag
    68281 aattgaaaaa gaaaatcgac aatcgaacag aaacgttagt tcaaaatgtg ttgaatgcga
    68341 cggcatggat cactggtaat tctggtgggc atgtcgtttt tcgtccagaa aaagctccat
    68401 cagagattct tatcatggat actccaaatg tggcgaatgc aaagcgagtg tggcgttgga
    68461 atttaaatgg cttaggttat tcagacaacg gagtaaatgg cccgttcggt attgctatca
    68521 catcaa
//
LOCUS       DASJPH010000002        68079 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJPH010000002 DASJPH010000000
VERSION     DASJPH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.002"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(381..2016)
                     /locus_tag="U3995_000091"
                     /pseudo
     CDS             complement(381..2016)
                     /locus_tag="U3995_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2339..2818)
                     /gene="rlmH"
                     /locus_tag="U3995_000092"
     CDS             complement(2339..2818)
                     /gene="rlmH"
                     /locus_tag="U3995_000092"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1795446.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3246..4535
                     /locus_tag="U3995_000093"
     CDS             3246..4535
                     /locus_tag="U3995_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1795447.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4574..5179)
                     /locus_tag="U3995_000094"
     CDS             complement(4574..5179)
                     /locus_tag="U3995_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1795448.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5384..5848)
                     /locus_tag="U3995_000095"
     CDS             complement(5384..5848)
                     /locus_tag="U3995_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1795449.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5870..6190)
                     /locus_tag="U3995_000096"
     CDS             complement(5870..6190)
                     /locus_tag="U3995_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1795450.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6334..7413)
                     /gene="pepA"
                     /locus_tag="U3995_000097"
     CDS             complement(6334..7413)
                     /gene="pepA"
                     /locus_tag="U3995_000097"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1795451.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7613..7963
                     /locus_tag="U3995_000098"
     CDS             7613..7963
                     /locus_tag="U3995_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1795452.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8186..9133
                     /locus_tag="U3995_000099"
     CDS             8186..9133
                     /locus_tag="U3995_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1795453.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9190..10608)
                     /gene="pepV"
                     /locus_tag="U3995_000100"
     CDS             complement(9190..10608)
                     /gene="pepV"
                     /locus_tag="U3995_000100"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1795454.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10757..11602
                     /locus_tag="U3995_000101"
     CDS             10757..11602
                     /locus_tag="U3995_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1795455.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11606..12046
                     /locus_tag="U3995_000102"
     CDS             11606..12046
                     /locus_tag="U3995_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1795456.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12096..12290
                     /locus_tag="U3995_000103"
     CDS             12096..12290
                     /locus_tag="U3995_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795457.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12426..13112
                     /locus_tag="U3995_000104"
     CDS             12426..13112
                     /locus_tag="U3995_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1795458.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13520..14209)
                     /locus_tag="U3995_000105"
     CDS             complement(13520..14209)
                     /locus_tag="U3995_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795459.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14184..16313)
                     /locus_tag="U3995_000106"
     CDS             complement(14184..16313)
                     /locus_tag="U3995_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1795460.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16361..17698)
                     /locus_tag="U3995_000107"
     CDS             complement(16361..17698)
                     /locus_tag="U3995_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1795461.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17950..18309)
                     /gene="rplT"
                     /locus_tag="U3995_000108"
     CDS             complement(17950..18309)
                     /gene="rplT"
                     /locus_tag="U3995_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1795462.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18362..18562)
                     /gene="rpmI"
                     /locus_tag="U3995_000109"
     CDS             complement(18362..18562)
                     /gene="rpmI"
                     /locus_tag="U3995_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1795463.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18634..19158)
                     /gene="infC"
                     /locus_tag="U3995_000110"
     CDS             complement(18634..19158)
                     /gene="infC"
                     /locus_tag="U3995_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1795464.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19202..19327)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19478..20110
                     /locus_tag="U3995_000111"
     CDS             19478..20110
                     /locus_tag="U3995_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1795465.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20112..21017)
                     /locus_tag="U3995_000112"
     CDS             complement(20112..21017)
                     /locus_tag="U3995_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1795466.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21102..21761)
                     /locus_tag="U3995_000113"
     CDS             complement(21102..21761)
                     /locus_tag="U3995_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1795467.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22077..23021
                     /gene="mvk"
                     /locus_tag="U3995_000114"
     CDS             22077..23021
                     /gene="mvk"
                     /locus_tag="U3995_000114"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1795468.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23014..23991
                     /gene="mvaD"
                     /locus_tag="U3995_000115"
     CDS             23014..23991
                     /gene="mvaD"
                     /locus_tag="U3995_000115"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1795469.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24004..25089
                     /locus_tag="U3995_000116"
     CDS             24004..25089
                     /locus_tag="U3995_000116"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1795470.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25086..26150
                     /locus_tag="U3995_000117"
     CDS             25086..26150
                     /locus_tag="U3995_000117"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1795471.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26399..27202)
                     /locus_tag="U3995_000118"
     CDS             complement(26399..27202)
                     /locus_tag="U3995_000118"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1795472.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27222..28064)
                     /gene="kduI"
                     /locus_tag="U3995_000119"
     CDS             complement(27222..28064)
                     /gene="kduI"
                     /locus_tag="U3995_000119"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN1795473.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28080..29087)
                     /locus_tag="U3995_000120"
     CDS             complement(28080..29087)
                     /locus_tag="U3995_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1795474.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29094..29738)
                     /locus_tag="U3995_000121"
     CDS             complement(29094..29738)
                     /locus_tag="U3995_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1795475.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29838..30596)
                     /locus_tag="U3995_000122"
     CDS             complement(29838..30596)
                     /locus_tag="U3995_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN1795476.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30723..31874)
                     /gene="fucO"
                     /locus_tag="U3995_000123"
     CDS             complement(30723..31874)
                     /gene="fucO"
                     /locus_tag="U3995_000123"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN1795477.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31956..32267)
                     /gene="rhaM"
                     /locus_tag="U3995_000124"
     CDS             complement(31956..32267)
                     /gene="rhaM"
                     /locus_tag="U3995_000124"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN1795478.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32269..33114)
                     /gene="rhaD"
                     /locus_tag="U3995_000125"
     CDS             complement(32269..33114)
                     /gene="rhaD"
                     /locus_tag="U3995_000125"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN1795479.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33128..34384)
                     /gene="rhaA"
                     /locus_tag="U3995_000126"
     CDS             complement(33128..34384)
                     /gene="rhaA"
                     /locus_tag="U3995_000126"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN1795480.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34381..35841)
                     /gene="rhaB"
                     /locus_tag="U3995_000127"
     CDS             complement(34381..35841)
                     /gene="rhaB"
                     /locus_tag="U3995_000127"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN1795481.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35994..36863
                     /locus_tag="U3995_000128"
     CDS             35994..36863
                     /locus_tag="U3995_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN1795482.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36989..37888)
                     /locus_tag="U3995_000129"
     CDS             complement(36989..37888)
                     /locus_tag="U3995_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1795483.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38053..38877)
                     /locus_tag="U3995_000130"
     CDS             complement(38053..38877)
                     /locus_tag="U3995_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1795484.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38888..40183)
                     /locus_tag="U3995_000131"
     CDS             complement(38888..40183)
                     /locus_tag="U3995_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN1795485.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40199..40678)
                     /locus_tag="U3995_000132"
     CDS             complement(40199..40678)
                     /locus_tag="U3995_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1795486.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40684..41712)
                     /locus_tag="U3995_000133"
     CDS             complement(40684..41712)
                     /locus_tag="U3995_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN1795487.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41729..42835)
                     /locus_tag="U3995_000134"
     CDS             complement(41729..42835)
                     /locus_tag="U3995_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1795488.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43064..45661)
                     /gene="adhE"
                     /locus_tag="U3995_000135"
                     /gene_synonym="adhC"
     CDS             complement(43064..45661)
                     /gene="adhE"
                     /locus_tag="U3995_000135"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1795489.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45966..46346)
                     /gene="yajC"
                     /locus_tag="U3995_000136"
     CDS             complement(45966..46346)
                     /gene="yajC"
                     /locus_tag="U3995_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1795490.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46443..47588)
                     /gene="tgt"
                     /locus_tag="U3995_000137"
     CDS             complement(46443..47588)
                     /gene="tgt"
                     /locus_tag="U3995_000137"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1795491.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47806..48504
                     /locus_tag="U3995_000138"
     CDS             47806..48504
                     /locus_tag="U3995_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1795492.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48672..51311)
                     /locus_tag="U3995_000139"
     CDS             complement(48672..51311)
                     /locus_tag="U3995_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1795493.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51930..52961)
                     /gene="queA"
                     /locus_tag="U3995_000140"
     CDS             complement(51930..52961)
                     /gene="queA"
                     /locus_tag="U3995_000140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1795494.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53154..54328
                     /locus_tag="U3995_000141"
                     /pseudo
     CDS             53154..54328
                     /locus_tag="U3995_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            54332..54973
                     /locus_tag="U3995_000142"
     CDS             54332..54973
                     /locus_tag="U3995_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795495.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54970..55887
                     /locus_tag="U3995_000143"
                     /pseudo
     CDS             54970..55887
                     /locus_tag="U3995_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            55884..56555
                     /locus_tag="U3995_000144"
     CDS             55884..56555
                     /locus_tag="U3995_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795496.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57096..57584)
                     /locus_tag="U3995_000145"
     CDS             complement(57096..57584)
                     /locus_tag="U3995_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1795497.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57785..58051)
                     /locus_tag="U3995_000146"
     CDS             complement(57785..58051)
                     /locus_tag="U3995_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1795498.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58051..58314)
                     /locus_tag="U3995_000147"
     CDS             complement(58051..58314)
                     /locus_tag="U3995_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1795499.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58790..58975
                     /locus_tag="U3995_000148"
     CDS             58790..58975
                     /locus_tag="U3995_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1795500.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59117..60940)
                     /locus_tag="U3995_000149"
     CDS             complement(59117..60940)
                     /locus_tag="U3995_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1795501.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61538..64243)
                     /locus_tag="U3995_000150"
     CDS             complement(61538..64243)
                     /locus_tag="U3995_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1795502.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64549..65496)
                     /locus_tag="U3995_000151"
     CDS             complement(64549..65496)
                     /locus_tag="U3995_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1795503.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65686..65955
                     /gene="rpsN"
                     /locus_tag="U3995_000152"
     CDS             65686..65955
                     /gene="rpsN"
                     /locus_tag="U3995_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1795504.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66131..66493)
                     /locus_tag="U3995_000153"
     CDS             complement(66131..66493)
                     /locus_tag="U3995_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1795505.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66504..67625)
                     /locus_tag="U3995_000154"
     CDS             complement(66504..67625)
                     /locus_tag="U3995_000154"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1795506.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67641..67991)
                     /locus_tag="U3995_000155"
     CDS             complement(67641..67991)
                     /locus_tag="U3995_000155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1795507.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT        19577 a        14492 c        12435 g        21575 t
ORIGIN      
        1 tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
       61 catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta
      121 gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga
      181 ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc
      241 ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat
      301 tattataata taaagtttaa atttggttta ttacttactt tttcttattc tctagtaagc
      361 taatattgaa aaaaatgtaa tcagatcatt atttttatat gttgtaactt cctgataaga
      421 aaactaatta aaagaaagag gtaactacat aaactaatta aaaaagctaa cttcatagtt
      481 ggaggataaa tggaagaaac ttcaatgatt gtttctcctt gtgttccttc aatcgacact
      541 agtcctatta tcaatctctc ttttttgtcc atttactttt gcgactgttc cccaataatc
      601 aaatacagga agcgttattt tttgtccttt agattcatgt actgtataga ctattttcga
      661 atccatcact tgataaggca tagtatgcca ttccttgcca attcttactt catgattctg
      721 caaatgttct ttgtattgta atgcttccgt gggtgcatag tcataaagtc ctccgggaag
      781 gagtgtcgtg taacgaataa gattttcttg attcattacc aagtgatctc tctgagtgac
      841 aactttatgt tcatagtttg cttttgttgc agcagataca gccaacagta ataaaacaat
      901 tgccgttata ttaactactt ttctttgctt agctgatgtg ctcttcatga aaacgaacga
      961 taaaatcaac gagccaaaca gtacttgaaa gcctaagatt cgccaaggaa attgaatcat
     1021 ttgaatgggt gtattttgta ataaagacca tggaaaaaat ttggtggata agagccaaga
     1081 gactaccgca ctaaggtaaa tcactttttc tttgcgatta aatcctttaa aaaagaagag
     1141 tggacagatc aaagaaataa taccaatgat tcctaccgta aaggttgtca ggtcattatt
     1201 catcgcacca atcaaactat ccgagacatt taatgcttgg tgttgtaagg tcgttttatc
     1261 tggctcatta atcgattgaa ccatgatttg tcggaacatc ggataccaaa agtatgctgt
     1321 taaaagaatc gtcattccta ctgcctttga caactccaca atcaaccgga tgctttcttt
     1381 ttttcttgtt tcacttacta caaagccgcc taagactagt aagccaacgg ctattacact
     1441 cataaaagtg gaaagaacat gtgtataaat gagtaagctc atactgactg ctaatggaat
     1501 tgcacatggc tttttctcat aaatcagtgc gtaagcataa tacataacta tcggtataac
     1561 cgtaagtgca atcgcttcag caatagcaga acgatgataa atatcaatcg tacggtaccc
     1621 ataaaatgta tataaacagc taaatataaa agccgcatcg attcttttta aaaattttag
     1681 tccataatga aacatcagca tgatagtaca aacattcaat agccaaacat ataagatata
     1741 actaacgatt aaattattac ttatccaata aaaaatgact gccggaaaaa aggtcaagta
     1801 gggataaaaa taattcactc cgctcccata atgattaaac gtcgtaaaat tgattggacc
     1861 agaaaaaatt gaagataacc ctttgactct agcaaaatgg aaggcaatat cccccgatgt
     1921 agaaatagta tgaacatttc taaaataatt gattgaataa agtccagcaa taattgctag
     1981 gagaatggac aatattattt ttttctttac ttgcattact taattatata ctcctttcat
     2041 ggattgcttt atctagtata acttaaattt taaataaatc gttatttatt tttactctca
     2101 cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt
     2161 ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac
     2221 cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata
     2281 tccgctttct tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc
     2341 acttatgata aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga
     2401 tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc
     2461 ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt
     2521 ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc
     2581 cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc
     2641 cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt
     2701 caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga
     2761 taccttggat caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt
     2821 tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat
     2881 gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag
     2941 gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat
     3001 acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt
     3061 tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt
     3121 tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta
     3181 gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg
     3241 aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct
     3301 ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca
     3361 ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga
     3421 attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca
     3481 aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc
     3541 aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata
     3601 gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg
     3661 cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga
     3721 aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg
     3781 tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc
     3841 tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt
     3901 ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga
     3961 acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg
     4021 gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa
     4081 gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta
     4141 acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc
     4201 taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga
     4261 agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg
     4321 ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt
     4381 cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa
     4441 tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat
     4501 cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact
     4561 tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta
     4621 agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc
     4681 catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag
     4741 ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga
     4801 tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag
     4861 gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa
     4921 gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg
     4981 ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc
     5041 aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag
     5101 tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt
     5161 tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca
     5221 aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct
     5281 ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact
     5341 gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt
     5401 cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta
     5461 cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat
     5521 cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa
     5581 taattccttc tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca
     5641 tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca
     5701 taaagttata aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc
     5761 gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta
     5821 ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg
     5881 ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac
     5941 gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt
     6001 cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt
     6061 cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag
     6121 taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg
     6181 ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc
     6241 tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa
     6301 tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac
     6361 tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc
     6421 gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt
     6481 acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac
     6541 aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg
     6601 aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc
     6661 gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag
     6721 atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac
     6781 attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc
     6841 taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt
     6901 ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact
     6961 cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga
     7021 tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata
     7081 attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca
     7141 gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc
     7201 gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg
     7261 cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg
     7321 actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa
     7381 ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa
     7441 actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg
     7501 tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt
     7561 gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga
     7621 agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt
     7681 gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt
     7741 acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc
     7801 ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct
     7861 ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg
     7921 aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag
     7981 agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt
     8041 ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat
     8101 atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt
     8161 tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc
     8221 ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc
     8281 agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt
     8341 tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca
     8401 aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc
     8461 tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat
     8521 tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt
     8581 tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt
     8641 catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc
     8701 tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt
     8761 tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt
     8821 ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt
     8881 gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca cggggattcc
     8941 tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag
     9001 tactggattg atgactttag cacttctttc attagtaaca gggttgattt tggatacagt
     9061 cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca
     9121 cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt
     9181 tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa
     9241 caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa
     9301 catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat
     9361 ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa
     9421 tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc
     9481 gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt
     9541 tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc
     9601 aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag
     9661 tttttctcca taaaaatctt catggacata agcagctgct acatggataa attgtttagc
     9721 agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc
     9781 agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt
     9841 gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc
     9901 catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac
     9961 catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc
    10021 tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc
    10081 atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc
    10141 ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc
    10201 aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct
    10261 tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca
    10321 gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc
    10381 gccaagatca atatgccctg catagttatc gacatttttt actttaaatc catcacgttc
    10441 gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg
    10501 tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag
    10561 caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct
    10621 ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa
    10681 aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt
    10741 aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg
    10801 tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca
    10861 atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac
    10921 gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat
    10981 gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact
    11041 cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact
    11101 agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc
    11161 tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga
    11221 gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa
    11281 gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca
    11341 ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga
    11401 cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat
    11461 tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat
    11521 tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa
    11581 atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt
    11641 ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga
    11701 agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc
    11761 tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg
    11821 agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt tggattttag
    11881 ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg
    11941 ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga
    12001 tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa
    12061 gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc
    12121 ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg
    12181 aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta
    12241 aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt
    12301 ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta
    12361 tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag
    12421 gttaaatggt tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca
    12481 tttattccga gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag
    12541 ctatcgggcg tacaccgatt caagaagcac tgatccaact gcgcaatcaa gagcttgtaa
    12601 aaaccattcc acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag
    12661 cccgttatac gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga
    12721 ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg
    12781 ctcaaaataa aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa
    12841 ttgctggaaa acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt
    12901 tcagatggtt acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc
    12961 agacaatgct tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga
    13021 tgcatttaca tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt
    13081 ttgaagatat tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg
    13141 agtattagag aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa
    13201 taccgagggt cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca
    13261 tcaagtaata agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt
    13321 attaccgcag atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca
    13381 tctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga
    13441 cttgtgaccg aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac
    13501 gacttttgtc acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta
    13561 ttttttttat ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga
    13621 ctgcctctaa tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg
    13681 ctgctatctc gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca
    13741 gcacaatttt ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata
    13801 gttcatgtcc aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac
    13861 attttaattt ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg
    13921 caaaagtgat tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt
    13981 gttcacaaat ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt
    14041 cgataccaca atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt
    14101 tccaaaataa atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta
    14161 attcgtttag taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact
    14221 tcttcctccg gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga
    14281 tccgtcgctg taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca
    14341 atcaattcag tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa
    14401 ttggctgcag ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt
    14461 tgatcgatga tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa
    14521 aagaaaggtg tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca
    14581 gcaagctcta gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc
    14641 gtagaaccag tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact
    14701 tcgatcgatt ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac
    14761 gatcgtatat cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt
    14821 gcaagtgcta caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca
    14881 cccattttat tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga
    14941 gaaatctgat ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc
    15001 ttagcatagg atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa
    15061 tcagctaatg cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct
    15121 tcttgcacta atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg
    15181 tcttccatca ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca
    15241 accttaggac ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata
    15301 tgtgcagaac gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg
    15361 cctgccacac ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca
    15421 attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg
    15481 cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat
    15541 gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt
    15601 actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa
    15661 tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct
    15721 gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg
    15781 atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca
    15841 aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct
    15901 gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt
    15961 ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct
    16021 aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca
    16081 ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt
    16141 tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg
    16201 tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact
    16261 gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt
    16321 tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata
    16381 agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca
    16441 tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa
    16501 tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg
    16561 acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat
    16621 tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta
    16681 atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag
    16741 ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg
    16801 cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt
    16861 ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat
    16921 ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt
    16981 cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata
    17041 tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga
    17101 aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat
    17161 attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga
    17221 acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa
    17281 tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata
    17341 gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg
    17401 tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta
    17461 ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa
    17521 ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc
    17581 ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt
    17641 accagaccat gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt
    17701 tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc
    17761 taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa
    17821 aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg
    17881 attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa
    17941 ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc
    18001 tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt
    18061 caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg
    18121 agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt
    18181 gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta
    18241 accttttgct aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac
    18301 acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt
    18361 cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc
    18421 ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa
    18481 acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc
    18541 ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact
    18601 atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag
    18661 cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc
    18721 agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg
    18781 gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt
    18841 acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac
    18901 attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta
    18961 tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag
    19021 atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg
    19081 ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat
    19141 atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata
    19201 aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc
    19261 agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct
    19321 tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac
    19381 agtttgtctt ccttttctga aaatgattag attagcagac tttcttgcag tgattcccgc
    19441 ttctttgcta tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg aacctattcc
    19501 accttctctt cctcttctta caaagggaga gttttattta gaagatgagg agatcttctc
    19561 tggtcttctg atcaacgggg gtgaccaaag ctatctcgat gccaaaaata tggtattaag
    19621 agagagtcat atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag
    19681 taatgtcatt tttgaaaaat gtgatttttc caatctggag tggattggtg gcagtttcca
    19741 ccaagtcgtg tttcatcaat gtaaattaac aggtacgaat tttgccgaaa gttatcttcg
    19801 agactgtact ttcactgatt gtattgcttc gatggcttcc tttagtagta caaacttgaa
    19861 agctgtctcc ttcgatcatt gtcaattaga agacagcgaa ttttatgagg tcacttggaa
    19921 aaaccttttt ttgtctgaca atcagttgac cggttcgaac tggtttcgaa cttctctgaa
    19981 gggattagac tttacgacta atttcttcac agcaatcgct ttatctcaag attctttggc
    20041 gggactaatc gtcaaccaag agcaagcact tgtgatcgcc caagcccttg gtttgcagat
    20101 caaagactag atcaacgaat ccgccgccac agtgcaataa gcatccaaat agataagacc
    20161 aaactgatga tcaaggcgat tccccatccg aaaacggaat gggtaaaggg taactggaga
    20221 ttcattccga agaatcctgt cacaatagtt ggaacggtca agatcagcga ccagaccgtc
    20281 aaaaatttca tcgtgtcatt caagttgtta ttcagcaaat tattataagt gccagagagc
    20341 tggtctaaaa tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc
    20401 gcatcgtcca actcttcttt ttccttttct gacaacttgc gataaatagc caatgccttg
    20461 atttgttcta aaagtactac attttgtttt gttcctgtaa ctaagtaaac caaaccgatc
    20521 tctaaatctg aaagttgcag cagattcttg ttcgttgttt tttctcttaa tttcagatta
    20581 agccgctgac gttcactatt cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa
    20641 aacaatgtac gaaacaataa actgtataca ctcatgtctg gcattcgttc caaaagcgat
    20701 cgaattagtc gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct
    20761 tttaaaataa aagcgatagg actagtttca taatgattat cgactttttg ctggtgcgga
    20821 acatttgata caagcagtaa cgcttcatca aaagaatcgt actctacgcg cgcccgttca
    20881 tttttatcta gagaataagt cagcatctcg tcactaatgc catattcttc ttttaagttt
    20941 ttaacagcta gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa
    21001 tagtgttttt cccgcatact ctatcctcct gacatttgtt tccagtataa agaaaaaagt
    21061 cgctcaaata aaggtttacc ctttatttga gcaactgtaa atcaaatggc tactttggtt
    21121 tggcgaatct ctgctaattt ttcttcgatc attttcaaga cttcttcttt tgcttgagga
    21181 tcttccacaa aattatagcg gtctccatcg atttggattt ttggactttc tttgtattct
    21241 tcataccatt gatcgtaacg tctgttcaat tctttgtaat aatcgtatag acttggatca
    21301 aaggacaatt gttcatacga acgaccacgt ttttcgatac gctccaacat cgtatcaaaa
    21361 gatacacgaa tatgaactaa taaatccgga tgttttttat gggcagcata gggaagttct
    21421 tccatcatgt tttccagcag ttcatcatac acacgaactt ctgtttctgt tgctcttcct
    21481 agatctgcat ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc caatacatta
    21541 ttgtcatctt tcattgcttg tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc
    21601 agcaaaaaag catactgttc agggtttgca taaaataagg gcaagacttc attatcatca
    21661 atcgattcat aaaatgcctg actatcaaag tgttcagcca tcatttctgt taaacttgat
    21721 tttccggcac caattgtacc tgctaaaacg atcacgctca tttacctcca gctcacttca
    21781 tcactaaata ttgtgtttaa ctccaacctt tttaaatata cccccaatat ctagtagtgt
    21841 caattgtatt tttttctagt caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt
    21901 gttttttctt tagaaaacgt attcttagtt ttacttctga gatggtttta gtaaaataaa
    21961 ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt
    22021 tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg
    22081 caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg
    22141 tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg
    22201 aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg
    22261 aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg
    22321 acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg
    22381 gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc
    22441 ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccatggta
    22501 atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag
    22561 ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag
    22621 gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc
    22681 ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag
    22741 caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc
    22801 tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata
    22861 ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag
    22921 cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg
    22981 aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat
    23041 accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg
    23101 aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt catcttttct
    23161 gatagctata tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca
    23221 aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct
    23281 tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg
    23341 ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct
    23401 ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc
    23461 gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat
    23521 tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg
    23581 tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta
    23641 gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa
    23701 cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc
    23761 cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa
    23821 ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt
    23881 ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa
    23941 gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt
    24001 tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt
    24061 gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa
    24121 tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg
    24181 acaagacgca acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt
    24241 gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat
    24301 gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct
    24361 agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta
    24421 tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt
    24481 tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac
    24541 catccttggc tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat
    24601 tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt attgattggt
    24661 tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa
    24721 gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg
    24781 atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga
    24841 caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac
    24901 aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg
    24961 ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca
    25021 tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa
    25081 aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa
    25141 aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg
    25201 atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg
    25261 cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa
    25321 gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt
    25381 tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca
    25441 tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg
    25501 ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt
    25561 ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg
    25621 taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag
    25681 ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc
    25741 gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat
    25801 tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca
    25861 atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa
    25921 ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat
    25981 ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac
    26041 accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg
    26101 acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt
    26161 tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag
    26221 ctaaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
    26281 aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg
    26341 tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt
    26401 aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa
    26461 cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc
    26521 cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt
    26581 tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc
    26641 cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta
    26701 atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg
    26761 agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg
    26821 aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca
    26881 tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac
    26941 ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc
    27001 agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg
    27061 attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa
    27121 tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa
    27181 acatatccat actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt
    27241 tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag
    27301 taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca
    27361 ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta
    27421 tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa
    27481 ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag
    27541 ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct
    27601 tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga
    27661 tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat
    27721 acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt
    27781 tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc
    27841 tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta
    27901 ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct
    27961 ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg
    28021 atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc
    28081 tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat
    28141 tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg
    28201 atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct
    28261 ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc cacatattca
    28321 tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg
    28381 gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt
    28441 agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt
    28501 tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct
    28561 tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt
    28621 tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg
    28681 attcctgaaa catgaaatat atctattcct tggaataact cctctagcga ccattccaaa
    28741 tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg
    28801 ccactaccag tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc
    28861 gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt
    28921 acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct
    28981 tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct
    29041 gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc
    29101 ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct
    29161 cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg
    29221 aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc
    29281 atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg
    29341 acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt
    29401 aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta
    29461 acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg
    29521 cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact
    29581 tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt
    29641 gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg
    29701 gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt
    29761 agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat
    29821 tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg
    29881 agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca
    29941 ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca
    30001 cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt
    30061 ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca
    30121 agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa
    30181 ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga
    30241 tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca
    30301 tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt
    30361 tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa
    30421 tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag
    30481 gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag
    30541 tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa
    30601 tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat
    30661 atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt
    30721 ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt
    30781 cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt
    30841 acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca
    30901 ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga
    30961 acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt
    31021 ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga
    31081 gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt
    31141 cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg
    31201 ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg
    31261 ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc
    31321 ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca
    31381 acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct
    31441 gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca
    31501 gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg
    31561 gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc
    31621 tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg
    31681 tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata
    31741 ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga
    31801 aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc
    31861 attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa
    31921 gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat
    31981 tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac
    32041 catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact
    32101 aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg
    32161 tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct
    32221 tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc
    32281 tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa
    32341 atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt
    32401 atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt
    32461 tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc
    32521 agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc
    32581 ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg
    32641 ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac
    32701 gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa
    32761 ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc
    32821 ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata
    32881 tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat
    32941 cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag
    33001 taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt
    33061 cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc
    33121 cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc
    33181 aatccaaacc aactggaact tgatttttgt aacagaaata attccataca tcggcatacg
    33241 ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca
    33301 tttctttcaa tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc
    33361 tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga
    33421 tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat
    33481 catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga
    33541 ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa
    33601 aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt
    33661 catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat
    33721 cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac
    33781 gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt
    33841 tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt
    33901 tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt
    33961 ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt
    34021 ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt
    34081 cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat
    34141 aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc
    34201 ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat
    34261 ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg
    34321 tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt
    34381 tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc
    34441 atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat
    34501 attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc
    34561 actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg
    34621 attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc
    34681 tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat
    34741 agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc
    34801 tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg
    34861 tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg
    34921 ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg
    34981 ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt
    35041 tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg
    35101 acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac
    35161 atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg
    35221 ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc
    35281 ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac
    35341 catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt
    35401 tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg
    35461 aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc
    35521 tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc
    35581 aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt
    35641 ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg
    35701 atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa
    35761 agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat
    35821 ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat tagcacttta
    35881 ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt atattttgct
    35941 tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt
    36001 actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta
    36061 cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa
    36121 tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg
    36181 gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt
    36241 ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg
    36301 attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg
    36361 gccataacac cttccattca acaggagatc catcagaaaa caagatccac catttgatca
    36421 cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag
    36481 ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag
    36541 aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa
    36601 cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag
    36661 atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt
    36721 acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag
    36781 ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga
    36841 gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc aaaatatttt
    36901 gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa caacagcagc
    36961 tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt tcataatgaa
    37021 ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg aactctctct
    37081 tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat acagcactag
    37141 agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc attacatact
    37201 gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat ttgctgacaa
    37261 aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt gagatatatt
    37321 gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc gcactctctt
    37381 ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt tcatatttta
    37441 tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat ttttgcttca
    37501 atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt tgaaaacaag
    37561 ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg acaaagatat
    37621 agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca ttggaatgga
    37681 tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt
    37741 ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc gttttatttt
    37801 ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc gtattgactt
    37861 gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca cttatgcaca
    37921 attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac tcttataaaa
    37981 aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta gttaaccaac
    38041 gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat cttttgtttc
    38101 ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga tttttgtgta
    38161 ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga cacgaaaatc
    38221 tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt ctaccaattg
    38281 gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa tgattcctag
    38341 atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat acaccggatg
    38401 attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt ttaccaagcg
    38461 acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt ccgtatattg
    38521 gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa aggaagaagc
    38581 atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta aatctggatg
    38641 aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt ctaaaactcg
    38701 tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta tttcaggaaa
    38761 cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt tttctgcaag
    38821 atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt tcaacataac
    38881 tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag taagtaatca
    38941 aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc attgcctctg
    39001 cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc ggcgtgatgg
    39061 agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca acccctacac
    39121 ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc acatccataa
    39181 acatacccac cagtaataac acaagattga ctaataacag aataataaat ttattatccg
    39241 ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag
    39301 acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct actgattgaa
    39361 acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata gcaatcaaca
    39421 gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc gtcaagatac
    39481 caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt atttttccag
    39541 catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca acgatgaatg
    39601 ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc ataaacaaag
    39661 cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta cttggaggaa
    39721 tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc
    39781 cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg gcaatggccg
    39841 agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc aaagcccccg
    39901 gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc cctccattat
    39961 tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac
    40021 ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata actaacaata
    40081 aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat acaagcaaca
    40141 caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc ctccattttt
    40201 atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg atcaggctat
    40261 agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc ataggaatac
    40321 ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg ccatacacca
    40381 tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa attttacttt
    40441 ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa
    40501 catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga
    40561 ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca acagcgacca
    40621 tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc attttcatca
    40681 cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag gatcgttttt
    40741 gaatatatca tgaagtgggg caactgcctc tcgaaacggt gtattatcta catcattgaa
    40801 ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc
    40861 ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt cctgctcatc
    40921 agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac gtgtatgctc
    40981 atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga caaattcatt
    41041 gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat cacttcccat
    41101 aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc tttgcatcac
    41161 acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta ccatataaaa
    41221 gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt tggtcgattg
    41281 gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt cttcatcatt
    41341 gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca acgctgaggc
    41401 acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc
    41461 acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt tttcttttac
    41521 taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg cataagcaaa
    41581 gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac tagaattatt
    41641 tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa acagcagtga
    41701 tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat tctgtcaaac
    41761 aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc cgataaaaat
    41821 ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct ttttcatcga
    41881 tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct tgtggtaaat
    41941 aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca gaagattcta
    42001 cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac tgataagttt
    42061 ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt
    42121 tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag cagtttccac
    42181 gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg tgataccaca
    42241 atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa cgagcttctt
    42301 ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc actgtcatca
    42361 tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca tgctgtaacc
    42421 cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt tctaaataag
    42481 gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat agcggtgcca
    42541 ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac cattcattga
    42601 ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata ccatatagac
    42661 cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc agtccatcaa
    42721 agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca acgagcaggt
    42781 caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata atcatcttta
    42841 cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata ccgtccacta
    42901 taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa ataacaaaag
    42961 taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt ttccaagttc
    43021 tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg ttgtaagcat
    43081 caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct gtcgtacatt
    43141 ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt tcttgtgtga
    43201 ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca taaacagccg
    43261 ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag aattttgcga
    43321 tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc gcatgttttt
    43381 gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt gtccgtccat
    43441 gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata cctaggaatg
    43501 cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca cgtgattcca
    43561 tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt gcttgtaggc
    43621 tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt gcatgtgtta
    43681 acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg aattgaggat
    43741 cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc acatgtgttt
    43801 cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat gttgtcggga
    43861 tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta cggatatcca
    43921 agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac atccacattc
    43981 ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct ggcttgaagt
    44041 ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt tcaacatctg
    44101 agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga actgtatcgc
    44161 agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca acgttttcca
    44221 ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt aatttgaacc
    44281 attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt acagcagaga
    44341 cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat gacggaatca
    44401 tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact aaaatacgac
    44461 aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa tggataactg
    44521 ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac gcatggtccg
    44581 tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat agcggataat
    44641 ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt acactgattc
    44701 ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg accgcatatt
    44761 ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt acgatataaa
    44821 cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc acgatcacag
    44881 cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc aaatcgttca
    44941 ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct ggacctacac
    45001 ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca cctgtcgcta
    45061 agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc gaaggatgtt
    45121 cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca cgtaccactc
    45181 gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg attggatttc
    45241 ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc gttgttggag
    45301 ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt tggacatctt
    45361 cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat tcggacgcat
    45421 acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct acagccatct
    45481 tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg atatggtcta
    45541 ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt tctgccaaat
    45601 catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt actttagcca
    45661 tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta tatcatacaa
    45721 tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt tttaaatata
    45781 aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt attattattt
    45841 atatgacaca cccgattatt caatattgag caatatatcc catgctctta tctctttctt
    45901 ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta aataagcttt
    45961 gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt ttcagttttt
    46021 acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc atcatttgtt
    46081 acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag gaagatccct
    46141 tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac tccatgtaat
    46201 ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt ttgacgttct
    46261 tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag cgcgataaac
    46321 atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta tctacaaaaa
    46381 acactttagc agaaaaatgc tgataacact agttttttac ttaattttta aaagattttc
    46441 ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa ggcttctcta
    46501 aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag attcaacaag
    46561 aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca tttgatcaaa
    46621 tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt ctcatcaatt
    46681 ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg agaagtcata
    46741 catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc tactccacgg
    46801 ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg tggtttgttt
    46861 tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt aggttccccg
    46921 acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc tttcgcgctt
    46981 tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc ttgacggtct
    47041 ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc tactgatttt
    47101 ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat catgatatca
    47161 gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat acgcgaaccg
    47221 ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc acttaatgaa
    47281 aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat aaatttatgc
    47341 agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg atatgtatta
    47401 cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt ttttactgta
    47461 gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg cgtgatcaat
    47521 tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg aatggcaggt
    47581 tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca ccatcataat
    47641 cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta atattctttt
    47701 ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga aatatttttt
    47761 gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca atcttctgca
    47821 gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc tgttttacta
    47881 aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat tcctattatc
    47941 ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac aacttcagga
    48001 ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc tttgttcatg
    48061 agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca gatcgaacca
    48121 ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat cagcttagct
    48181 gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc tggaatcatt
    48241 aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca agaaacagga
    48301 caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga tggttataaa
    48361 ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc attagcaact
    48421 gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc agcattttat
    48481 gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg ggtggcagga
    48541 gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa aaaggaggtg
    48601 tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa aatcaactta
    48661 gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt gatcagttca
    48721 ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt caaactgatg
    48781 tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt tgtaatagct
    48841 aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac tttgaagatc
    48901 gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa gatcaagaat
    48961 gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt atcgacataa
    49021 ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt tatggcaatt
    49081 ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg ctgatccatg
    49141 atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc agcaactaca
    49201 ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag catcgcaatc
    49261 aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc tttacgaata
    49321 ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa gttgtcatcc
    49381 gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc catggcacac
    49441 ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt catcgctacg
    49501 actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg cgttacccga
    49561 gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa ttgatgcagt
    49621 tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt cgctatggca
    49681 ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc ctttttcgct
    49741 cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat cagaccaatc
    49801 aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc gtcataagta
    49861 gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt aacgatcgcc
    49921 gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc tcctttggtg
    49981 attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg ttcagaatca
    50041 aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt atgataattc
    50101 tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac aaggtcgtca
    50161 tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat ttccatcgcg
    50221 ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca tacccggcgg
    50281 acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac attagcggtt
    50341 cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc catcttctgc
    50401 acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac agctgcaaca
    50461 gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag ttcaccaatc
    50521 acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc tagctgattc
    50581 aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc cgcgatctta
    50641 cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc tgcagcaacc
    50701 gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc ttcagcaata
    50761 aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc ttctacacgt
    50821 aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga ctctaaaaca
    50881 agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc gtctcgtatg
    50941 acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc tgcgtttcct
    51001 tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag cagtccttca
    51061 aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc tgctacaaga
    51121 aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat cgattctttc
    51181 ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac ttcttgtgaa
    51241 gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat tgtctgcttg
    51301 tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac ttattttact
    51361 ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg ttaagaaagc
    51421 gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta tatagatgta
    51481 aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt gtaagcaaac
    51541 aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa cctataaata
    51601 gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa tgcgatacgt
    51661 aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt ttcaaatgtt
    51721 gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac cgtgctttgc
    51781 atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag gtacaagaaa
    51841 gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa agcttttttg
    51901 ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc tccaaaacta
    51961 aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa ttctcttcct
    52021 gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt agttgagaaa
    52081 gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca tccgctgtct
    52141 gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat tgaagttgtt
    52201 ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc agccgcttct
    52261 tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc tacgctgaca
    52321 ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac accctttgct
    52381 tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc tgcagcagaa
    52441 ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg ttctttgata
    52501 tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat tccttcatac
    52561 tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc ttgcaatctg
    52621 ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt gatcaatgtc
    52681 tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc accagtatct
    52741 ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac tagcgcatca
    52801 ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc tccagttttc
    52861 cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg tgcgattaat
    52921 tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact cactttctat
    52981 catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc actttttcac
    53041 ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt cattgtttta
    53101 ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc accatgattg
    53161 aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag gacgtcaatc
    53221 tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc ggaaaaacga
    53281 cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt ttgattgatg
    53341 gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc tatgttatcc
    53401 aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt gtccaaaaat
    53461 tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt gatcttgtgg
    53521 aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt cagcagcagc
    53581 gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg gacgagccat
    53641 tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa gatttacaag
    53701 agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg attaaactag
    53761 caagccgtat cgccatcatg agtgaaggcc gtgtgatcaa tttgatacac cagaaaatat
    53821 tttacgtcat cctgcaaacg atttcgtaga agagttgatt ggagaagatc gcctactaca
    53881 agccaagcca aatgcgacaa gagtcggcga agttatgcta aatacagcaa tcacgattac
    53941 accggaaaaa tcattacaag aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt
    54001 gttagttacc gataattcga atgtattaaa aggatttatc gatgtagaaa cgttgaataa
    54061 aaaacgagga aaagtatcca gtgtcggcga cattttgaat aaacatgttt tttacgtaaa
    54121 agaatccgcc tatcttcgtg ataccttgca acgcatttta aaacgcgggc tgaaatacgt
    54181 gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg actcgtgcat cattagtgga
    54241 tatcgtctac gatgtgattt ggggagaaga tcccgcaact actgatgttg cagagtcagc
    54301 tactcctgaa gaatctaagg aggtctgacc tatgcagcag ttttttcatc agcacggttc
    54361 agaaattctc tcaaaaggtt ttgaacatct ttatatttca ggacttgctt tgatcattgg
    54421 tatcatcatt gctgtaccgc ttggtgtctt actgacacgt tttccaaaag ctgccacgat
    54481 tgtgattggc ttaaccagtg ccttgcagac aatcccttct cttgctttat tggcattgat
    54541 gatcccgcta tttggcgtag gtaaattacc tgcgattatc gcactcttta tttattcatt
    54601 attgccgatt ttacgtaata cgtacattgg gatgaaaaat gtcgattgga attatcggga
    54661 tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt ttttccgttg aattgcccat
    54721 tgctatgcca acgatcatgg ctggtattcg tttagctgcc gtttacgtga ttgcatgggc
    54781 tacacttgcc tcttatatcg gcgctggtgg tttaggcgat ttgattttta gtggactgaa
    54841 taattaccaa ccagatctca ttttggcagg tacgatccct gtaacaattt tagcactatt
    54901 ggctgattgg cttctaggtc tcttggaaca ccggctgaca ccgatcgcac ttagagagga
    54961 gaatgaagaa tgaaacgtat actaaaaatg accatggttc ttttctctct tcttatactt
    55021 gcgagttgtt ccttccctgg tcttgccagc aatacagatg aagacaccat ttcgatcaca
    55081 ggtggaatca cttcagaagc acagatctta gcaagtctag ttgccggaat ggtttaacat
    55141 tatacagata aaaataccgc aatcatcaac aatctggcta ctacaactat caaccatcaa
    55201 gcgatgatga acggagatgc ttcaatttca gcggctcgct acacaggaac agatttaacg
    55261 actacattga accttcctcc tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat
    55321 gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct atagttttga aaatacgtat
    55381 gtctttcttg ttcgaaaaga caccgcacaa aaataccatc tatctaaagt cagcgatctg
    55441 aaaaatgtag cggatgaatt agtagctggt gtagatactt cttggatcaa tcgtaaagga
    55501 gacggttatg acggcttcca agaaacttat ggcttctcct tcaattcgat attacctatg
    55561 cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac
    55621 tctacggatg gacggatcgc cagttatgat ctagtcatgt tggaagatga tcgtcaattc
    55681 ttcccaccat atgatgctgc tccagttgta gacaacaaga tattagctga tacgcctcat
    55741 ttaaaagaag ccttaaataa gttaggtcat accatcagta cggaaaaaat gcaggaattg
    55801 aactacgaag cagataataa tctagtggaa ccatcagtag tagcacaacg tttcttacaa
    55861 gaaaaccact attttgaagg gaagtgaagc aattggataa aatgaatgtc tttcaacaat
    55921 ttttctatta tttccaggaa aatggctcct atatctttgc acaatttatc cgtcacttcc
    55981 tgatttctat ctatggtgtc ctctttgcag cagttgtcgg gattccggtt gggatcatga
    56041 tttctaggag acgtaaatta gcaaactggg tgattcgttt ggcgaatatc atccagacga
    56101 tcccttctct agccatgatc tccattttaa tcattggatt aggcctggga gtcaatgtcg
    56161 tgattgttac tgtatttttg tattccctct taccgatcat caagaatacg tataccggga
    56221 tgatccaagt agacaaaaac atccttgatg tcggcaaagg catggggatg actgctagac
    56281 aacgactatt catggtagag ttaccactct ctgtttctgt tatcatggca ggcataagaa
    56341 acgcgttagt tgttgccatc gggataaccg cgatcggtgc attcgtcggt gctggtggtc
    56401 ttggagatat cattatccga ggaaccaatg caacagaggg cacatcgatt attttagcag
    56461 gtgcattgcc aactgcatta atggcgatca tcacggactg gcttttaggt attttggaaa
    56521 gacggctaga tccagccagc cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc
    56581 tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt catttttaag gcaataatcc
    56641 actctgattc ttccctcctt atgggcaaag agttgctttt cttagctatt tatccattcc
    56701 gtatactcca acttcatact gccaacgggt tcatatctgc atccaccgac taagtttttt
    56761 tcttatccgc taaggcattt tgccaatcca ttttcccatc actcgtcatg gaattcatac
    56821 cacatgatct ctaaatactc gtctgcttgt tctttgaatt gggcatcaat tcttacatcc
    56881 tttggcgtgg ctttcattta agccttttcc atcagtcctc gattgttcga tgccttcagc
    56941 tgcatcatca aaactggtga ctgccgtatc ggtaacttcg atcatctttt atacaatcgg
    57001 gaacttgcca tcctcatcct tggctaaaaa catatctagc cattccagct tctcgccatg
    57061 ggaggtttgc ggttcctaaa accggtacac gattcttatg gactgcatcc gtcgcaacta
    57121 gacttggcga tacgatgatc cttcaccaga agagcacccc cgtaaaccaa ttgatcgata
    57181 tattgccaat aagaaaagac attggcgtaa aagatatcga tccctcgtga tgcattgccg
    57241 ttgatactgc tattcatggc cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca
    57301 ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta aaggaacgac actggtattg
    57361 tatttcgtat ctgtatcttt tccaaaagac catgccaacg aatcctctgg gaaccaataa
    57421 gacgactccg gttgattgtc cattgctttt ttcaacatac ttttggctgt ttctcgataa
    57481 acgacatttc caattgtctt cgcctcactt tggcgataat cgatggtcgt tgccactgcc
    57541 aacactaaca acgatgctcc aattttactg attttccttg tcacacttct tcctccttgt
    57601 tttacataaa cccgtcaaca ttccattcct aaactcaaac aatgaccctt caaaggtgga
    57661 gatataggaa caaattaaag aaatcatgca ataaatccaa gaaggttggt tttagaacaa
    57721 aaaaagagtc tcctttttgg ggagactctt tgttaactat ctaaaaaggt tggctcttct
    57781 cacttcactc ataatgtgtc caggcagaat aaatttctac aactttattt tcctcatcaa
    57841 ctttgtaaac cactcgatgc tgtctattca gcctgcgtga gtatctgcct tcatgtgtag
    57901 gtctcagctt ttcaaaagac tgcgtgggca tatatggatc ttccttcaat gtctgaatca
    57961 cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt tcttaaatcc actttcgcag
    58021 agtttttgat tgcgactgta tagttactca tcaaagagct tcccaatcaa tatcatcgat
    58081 attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact ttatctaaag tacctgtttg
    58141 ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc agcccaatca atacagcatt
    58201 gtttccacta cgatcactaa taatctcgat ttcagtatga ttttcattta cctctttcaa
    58261 taactgataa aaatttttac gagcagtgct aggattcatc gttgtattag ccattgtctc
    58321 tcctcctttt tctgatataa ttatagcgta cttgattaag tacgtcaaaa aaatacaata
    58381 aagcacttaa attcagcaaa cttttcctct tttttacata gagaaaaaga ccccttttta
    58441 gggaggatct ttgtccacgc tcacttctta ctcctttacg actagcggta gccaaacgta
    58501 aagtaattgg taacaacgta tcatctttag caaaggtcga atccaaagcc tgtgagtcta
    58561 tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt gacttgattg atatcttgaa
    58621 taacctgagg atacgcacga aagatttgta acggttattc aaaactgatt ttggccgtca
    58681 agtcttctac tgcctcaaaa atgattgcct ctttcgtctc ctgtcattca tgttcactat
    58741 cgatttgata cttgcttaga ccgttgggtt tgtgaatcac caattaaaaa tggcactgag
    58801 ttatggtaac aaaaaatggt cacttgacga ggctgagatt cctacttatg aagacacatg
    58861 gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata ggaggctgta cattatttgg
    58921 caaaggattc ggaaattatg atcaagacat caaaatcaaa atgttttaca gctaaatagg
    58981 aagaataata acatttcata cgctgttccc atctttaatg gcttatagag ggcaccttta
    59041 gctgagaata agacaaaaca gaccctctag cttcaattaa gagctagagg gtctgttttt
    59101 gattttaaaa cttggtttat ttcttcaaat ggtagctata ggtgctgaca acgatatttt
    59161 cacgccaaga caatcgcaat cttaaccgca aacttgtttg gttatggaga atattctgcc
    59221 agcgtctgtt tggtacaaat tgaggaatca acacagtagt tgtatagttt tgtttcgctg
    59281 catttttact tacgatatct acataacgga taattggatt ttcaatcgag cgataagaag
    59341 agtggacgat tgaaaaacgg acatctggga aatgcttttt aaattctgct tcgatttctt
    59401 tttctttttc gacatcttcg tctagcgaaa catgcatcgc aacgacataa tcaccgatcg
    59461 aacgtgcgta attcaacgca ccgatattta cccgtgtcac attcccgact aatacaagaa
    59521 cggtattccc gtcataatca tgtagttttg catcattttc taaacgaagc tgttctgcta
    59581 cttttttata atgatcatgg atcttataaa aaatgaacat aacgattggc ataatgatga
    59641 agaaaggcca aatgtcccct aaccgataga caaacaaaat cgcaatgatt gcatatgaga
    59701 tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca tctttttcct tcttttttcc
    59761 atttgacgac catccctgtt tgagataaag caaatggaat gaacacgccg atagagtata
    59821 acggaatcaa gcgttctgtt gagccatgga aaataaacag caatacgatc gaacctagcg
    59881 ctagtgtgat aatcccattg gaatagccta gtcgatcccc acgatcttga tacatgtgag
    59941 gcatgaattt atcttttgcc agattgtatg caagaactgg aaaagcagaa aaaccggtgt
    60001 tagcagcaac tgctaaaatc agcgctgtag caaattgaag gacgtaatac aaaataccat
    60061 gtccaaagac agctttacca atttgagaca aaacagtgac ttctttttca ggaacgatcc
    60121 cataccaata gttgataaat gtgatcccca cgaagaagaa acctaagatc aatgccatca
    60181 tcgtcaatgt agcagcggca ttttttgctc ttggcttctt aaagaatggt acagcattac
    60241 taatcgcctc caccccagtt aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag
    60301 cgatagaaac acccggcaca gccgctcctg gaagtgcagt agcttgcaat ggcacagcac
    60361 ctgtcacgat attatataaa ccaacaacga tcagtaacga gatcacaagg ataaatgtgt
    60421 acaccggaaa taataggaac gatgcagatt ccctcagccc acgaagattc agcatcatca
    60481 gtagcaaaac gattgtcaca gaaatagcga cttgatgacc gtagagtgct ggcaccgctg
    60541 atgtgatcgc ttcagcacct gctgagacag atactgctac tgtcaacatg tagtcgatca
    60601 ataaagagcc accggaaatc aagccagcat tctttccaag attctcactg ctgacgacat
    60661 atgctccccc gccatgagga taagcatgga tgatttgacg atatgatagc gttaatgaaa
    60721 tcaataaaat aataacaaaa gcggcaatgg gtagcgaata ccagatggct gcagcagaca
    60781 aagcgactaa aacgacaacg atttgttccg taccatatgc aatagatgat aacgcatcag
    60841 acgacaataa tgccaaagca gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt
    60901 ttaaaggctt acctactaac aaccttttta agtaatccaa tttctaaact cctttacaca
    60961 caattttact tcaagtttta tagtcgaaaa tttatagtaa acaagatgtt atagtacgct
    61021 cactgacctt aaaagtcaat aaactttaag aaaataattg gaaaaaaaca gaaaaatatc
    61081 aagcgctttt tcaaacgctt taatacagca aaaatctaac atttttttat gggctttttc
    61141 tttgaattct ataataaaag gatagaactt gacttagaaa atcaagttct atcctttttg
    61201 atatcttaac ttttggaaga gagatgtaag gctgctttct acccatcatt cttactactc
    61261 gaagcatagc gcagttgtca caacctcttg ataaacggtg tttaaaagct tgcatctgcg
    61321 gtaataagcc caaccaaatt aaaaacataa gaagctgttt ttatttggtc gttcttatta
    61381 cttgatgcag agcagctttt ttacaacctc ttggtacacg gtgttcaatc agctgaccct
    61441 cggtattagc tcaaaaaaac ggcaaaatat gaggaactat ttttccattt ttttctctaa
    61501 tactcaggtc aaaacactga tttcacaacc tcttttttta atgaaaaata tggctctcat
    61561 gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa cggatagatt gccgttccta
    61621 cgattccaaa gacaacaaac aaaccgatca tgatccagat tgctttcgta gcatttggcc
    61681 aataaattcc tcctgcagat tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg
    61741 gattgatcat ttggaagaat ttccctgaga cttggatcgg atagttcccg cctccgccag
    61801 agatcgacag tactaagatg ataatagcga tccctttacc gacattatcg aataatgcag
    61861 ccaagacata gacgatcatc atgaacgcta aggcgatcaa gacagcaaat gacacgctgt
    61921 agaccggatt ttggacatct actcctagta agaaaatatt ccctaatgta acgatcaagc
    61981 tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg
    62041 taaaattgtt tcggtctttc tttcctaaat agtattctgt cgtagcaaca cttgaaagaa
    62101 gaacagcgcc gacccacaga catagtgcag tataaaacgg ggtgctagct gaaccgttat
    62161 ttgcgattgg ataaagcgca ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt
    62221 cttttgttgc gtctagtttc aataatttga tgacttcgcc tagatcgact tcttgttcgc
    62281 cttttcggat cgcgtctgct gctttttgga taccagcttt gatatttggc cagtcattgt
    62341 tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc tggcattttt tcatttactg
    62401 ttgtcaatgt accggtgatt tcatttttta gtgctggcca atcattttgg ataaaatcag
    62461 cggccaatcc taatttttgc tgaatgatcg gtaattcatt gttgtatagg tctgcccctt
    62521 cattgatgcc attgacgatg gtatccatat ttccattcag cattgtattg gcatcatgga
    62581 tttcttgtcc aattgcaggc atttcttttt gatatttttc taaaattgcc actgcattcg
    62641 ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc gatctggtcc gctttgctca
    62701 gtgcgccttg tgcagtccca atcgtatcga tgacttggtc tagtagagaa gtgacagtgg
    62761 aactgatttg acctgtattg ataccagcaa tgatgccatt gatattatta acaacggcat
    62821 caatttgatc tagcaattca tcaatagcct tcgtgtcttc attttgaaca gctgtgttca
    62881 aagcagatag gcggttttgc agattgctta gcaagggttt taattgattt agctgatcaa
    62941 taattggctg taatttcgta tttccagcac ttgtttgcaa tttagtcagg aactcgacca
    63001 gtttatcgat tgcattttgt tgttgagata ttccgtcaat aaagccgttc aatacattgc
    63061 tgatgttttt cttttcatca tcagtcagct taccatcttc aatagctgtt cttacagtat
    63121 ctattaacga acctgtcgtt gttgccactt gtccaattga cttcaatgta acatctacac
    63181 tgtttgtgat gcttggtatc gcatctttca gctgagaggc acctgatttt gtttggtttg
    63241 ctagatcact tgcctgattt tctaaatttt tcacatctgg caagatatct tgtacttgtt
    63301 ggatgatcgt caagccttgt tttgcttcat cgattccttg gttcattgtc tcttggatcg
    63361 aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc attttggatc tctgggattt
    63421 tttcttgcaa atcaaggatg atttttgctt gatctttcaa ctcaggcatt ttgtcattca
    63481 atgcaacgac tttttcaccc atctcatcta ctttagggat gtaatccacg aattcatttg
    63541 ctttattcag tttttccttg atttcaggta gttgactttg taattcgagg acttgttttg
    63601 tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa aatcatatct ttaactttat
    63661 tgatacttac taggttcgta tcgatgtcgt aacctatctc attgaatact ttcaataaag
    63721 tcgaactggc tgttttgata aattcatttg tgatttgcga ttggatcgaa cttgctcctt
    63781 tatcggtgat tttcggtgca atcgcattga ttttttcatt taccgtatat tcgatttttg
    63841 gtttcttgat atcaccacta gtaaaactca gcagatcttc tgagaaatcc tttggcaagt
    63901 aaatacctgc gtaatacttt ccggaacgga ctccgtcttc taattgttct tttgaatcaa
    63961 cgaattgcca tcctaattgt ttatttttat gaagcgattc gatgacttgc tccccaatgg
    64021 ccacttcttt accttgaaac tctgctggct tgtctgcact gtaaacagca attggcagct
    64081 ctccagtatt tccatatgga tcccataatg ctttgatgtt aaaccacgca tatagcgaag
    64141 ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc
    64201 agtcaagcaa atataatcga agcgtatttt tgattgactg catagttctc ccccttcttc
    64261 ttttttcagc tgaattactt taccatgtat ttcatacttt tttcaatcgg cttttttcaa
    64321 aaaagtcgaa tttttggaca tatcacggca attgtctcat tgtaatctct tacatattga
    64381 gaaataatta aggattacag cctttacttt atcatgaaat aaaagggaaa gataatttta
    64441 tgccaaagtt tttatctatt atacgtatac gcaaaaaagc taaaacattt tttgtctttg
    64501 cccctacaaa aatgtttcag cttttttcgt tttttattcc tgtatttctt atttttcgaa
    64561 tgacaggatg attttcccag caggaggatg tccttcaact agctgatgtg cttggatgac
    64621 atgcttaagt gaagcagaca gattcattga aataaaaaca tgaaaggctc ctttttggta
    64681 ggctttgatt atgccagcaa aggcttctgc atctagatat tcctttcttg gaacaaacga
    64741 ttcataaaag ccttcctttt tttgccgcaa atctagatct gggaggtcat ttaatgcaac
    64801 atacctgccg cctggtttca tgatctggat gccggtttct cctttgatac ttcctttcgt
    64861 cgcatcgatt acaaggtctg cctgatcagc aaattgcaga cctggatttg ttttatcata
    64921 agcagcaaat gctgaagctc ctaatttttt taccttttcc tcatttttac tggatgcact
    64981 ggttaagatt cggattccct tttcatggag aagctggatc agacttgacc cgaccgcacc
    65041 tgatgctcct agtatcatta cagtatctgt cggctggatt tccgtcaaat ggttgattag
    65101 attataagcc gtaattcctg gcgtgaccat tccggctgct tcttcccaac tcattttatc
    65161 tggaattttt gctacttttg ctgatggcaa cacgacttct tctccgtaag tcccgccgac
    65221 tgcatggaca gctactcgat cacctacatg aacagttgtg acatctgaac caatctctgt
    65281 cacaatacca gcgccatcat tacctggtac ataagggaat ttcaatgttc gtatctcttt
    65341 catttctcct aaacgcaagg caacatcata aggattcaca ctgaatgctt ttatttctac
    65401 acgaacatgt ccagaaccga cttctctggg atgagcatcg atttcttcaa aaacatcttg
    65461 tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc accatctccc tttttcttta
    65521 agtttacgtt tatgccttta ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt
    65581 caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa
    65641 agattacgat ttacgctaat aaaaaataat tggaggaatt tgttgatggc taaaaaatca
    65701 aagattgcca aagcaaaaaa acagcaagaa acgattgaac gctatgctgc tttacgccag
    65761 gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca
    65821 gaacgattga aaaatcgtga tttaatcgat ggacgccctc gcggatacat gcgtaaattc
    65881 gggatgtccc gtataaaatt ccgtgaatta gcacataaag gtttgattcc aggagtaaaa
    65941 aaagccagct ggtaatccaa gtctggctgt aatgtaaagg aggagggcgc atggaccact
    66001 ccttggatca tcaaaaaaga tacgttcgct tcttctagca gaaatgctgg tactagaagc
    66061 agacgtatct ttttttacgc acagctggtc cttttttata tgtaaaggct gcatggttac
    66121 ttcgctatat ttatttagta acaggagtca gtgcttcgat acccacacta attttcaatg
    66181 ctcggtcaac ttctgccata atgggtcgat ccaaatggca gaccttttct ttcaatcttg
    66241 atttatcgat cgttctgatc tgctccaaca ggataacgga gtctctttct attcctgttc
    66301 cagtagcttt gatcccaatg tgagtcggaa gcttaggttt agccattttt gccgttatgg
    66361 ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt attttgaata accaaaactg
    66421 ggcgtgttcc gccttgctcc gatcccacga ctggagaaag atcagcaaaa taaatatctc
    66481 ctctttttac cataggatcc tccttattct tgatattctc ttggtatccg ctgtcctaat
    66541 ccgcaagcca cttcatagtg gatcgtacct aattgatctg ctatatcctg catagtgatt
    66601 tctttgtctg catttttgcc gataagcgtc acttttgttc cgacaggaaa ttcttgaggc
    66661 aatcggatca tcagttgatc catacataca cgcccaatcg tttcacagta gttcccttct
    66721 accaatagag aaaaaccctg catctttcgt ggccagccat ctgcataacc gatcggtatc
    66781 gttccgatcc actctgcttc aggagtgata tatgtttctc catagccgat tccttcacct
    66841 gctgggagtt ttttgacttg aatcaattca gaaaccagct caagtgctgg ctgcaatgga
    66901 taaacttccg gtagtgcgtg cccagaggga ttaagtccgt acattgctac tccataacga
    66961 atcatattgc cgattgtttc atcatgccat aaagccgttg cactattgct gacatgtaca
    67021 tatcgaggaa gttcgggaag ttttttcagc acttctataa agcgttcttt ttgcagattc
    67081 caatatgtat catctgcctg atctgctgta gaaaaatggg taaataaacc ttcccattct
    67141 aatacacgac tttctttgat gaatgcagct gcctctttta cttcttcagg tgtacaaaat
    67201 ccgatacgac ccatcccagt gtctgctttt agatgaatcg aaagaggcgt ctctagctta
    67261 cttttctcta aaacagcagc tgctgcttgc aaccattccc gtgtacctgc agttactgat
    67321 aaatcatgag caacagctac agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg
    67381 ggctgagtaa atccagcttc tctcaactcg actccttcat ctaaatttga aacacaaaag
    67441 cccgaagctc cacctgcaac tgctgcctca gctgtttcaa tagctccatg accgtatccg
    67501 ttcgctttta ctactgcaaa caattctttt ccttgcggaa gtcttgcaac ttcatttgct
    67561 acattttcag ttatagcttt tttatgtatc actgctttcg ttgggcggtg ccaagcgaca
    67621 accatcgttt ttccttcttt ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt
    67681 gtatgagtaa tactgaccca tacattgcct tcatgaggtg aacgcgtcac aacaggctga
    67741 ccattttcat tttttaaaat ctcgacatct tgaaaggtaa cttttccgat cccagttccc
    67801 caagctttag agaaagcctc tttgcaagcg taacgcccgc ctaaaaattc tacttggcga
    67861 tgaaaaggca aggactggaa tagtttcatt tcatcagagg tcaagattcg tgcaatgaat
    67921 tttggttttt cttcgatcag ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt
    67981 ccttttatca tatgcttttc tcttttcaat aaatttagaa cactctcatt ctaccaaatt
    68041 ataaccattt tgccacaaat aagtccacaa ataacaaaa
//
LOCUS       DASJPH010000003        61969 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJPH010000003 DASJPH010000000
VERSION     DASJPH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.003"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1292)
                     /locus_tag="U3995_000156"
     CDS             complement(<1..1292)
                     /locus_tag="U3995_000156"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HEN1795508.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLT"
     gene            complement(1304..3349)
                     /gene="parE"
                     /locus_tag="U3995_000157"
     CDS             complement(1304..3349)
                     /gene="parE"
                     /locus_tag="U3995_000157"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1795509.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(3515..3949)
                     /locus_tag="U3995_000158"
     CDS             complement(3515..3949)
                     /locus_tag="U3995_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1795510.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            4076..4714
                     /gene="plsY"
                     /locus_tag="U3995_000159"
     CDS             4076..4714
                     /gene="plsY"
                     /locus_tag="U3995_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1795511.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(4846..5721)
                     /locus_tag="U3995_000160"
     CDS             complement(4846..5721)
                     /locus_tag="U3995_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1795512.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(5803..6594)
                     /gene="codY"
                     /locus_tag="U3995_000161"
     CDS             complement(5803..6594)
                     /gene="codY"
                     /locus_tag="U3995_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1795513.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(6612..8012)
                     /gene="hslU"
                     /locus_tag="U3995_000162"
     CDS             complement(6612..8012)
                     /gene="hslU"
                     /locus_tag="U3995_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1795514.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(8026..8574)
                     /gene="hslV"
                     /locus_tag="U3995_000163"
     CDS             complement(8026..8574)
                     /gene="hslV"
                     /locus_tag="U3995_000163"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1795515.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(8611..9516)
                     /gene="xerC"
                     /locus_tag="U3995_000164"
     CDS             complement(8611..9516)
                     /gene="xerC"
                     /locus_tag="U3995_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1795516.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(9714..11090)
                     /gene="trmFO"
                     /locus_tag="U3995_000165"
     CDS             complement(9714..11090)
                     /gene="trmFO"
                     /locus_tag="U3995_000165"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1795517.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(11059..13137)
                     /gene="topA"
                     /locus_tag="U3995_000166"
     CDS             complement(11059..13137)
                     /gene="topA"
                     /locus_tag="U3995_000166"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1795518.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(13259..14116)
                     /gene="dprA"
                     /locus_tag="U3995_000167"
     CDS             complement(13259..14116)
                     /gene="dprA"
                     /locus_tag="U3995_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1795519.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(14172..14939)
                     /locus_tag="U3995_000168"
     CDS             complement(14172..14939)
                     /locus_tag="U3995_000168"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1795520.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(14932..15792)
                     /gene="ylqF"
                     /locus_tag="U3995_000169"
     CDS             complement(14932..15792)
                     /gene="ylqF"
                     /locus_tag="U3995_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1795521.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16052..16450
                     /locus_tag="U3995_000170"
     CDS             16052..16450
                     /locus_tag="U3995_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1795522.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16729..17229
                     /locus_tag="U3995_000171"
     CDS             16729..17229
                     /locus_tag="U3995_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1795523.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(17312..17863)
                     /gene="lepB"
                     /locus_tag="U3995_000172"
     CDS             complement(17312..17863)
                     /gene="lepB"
                     /locus_tag="U3995_000172"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1795524.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(18015..18242)
                     /locus_tag="U3995_000173"
     CDS             complement(18015..18242)
                     /locus_tag="U3995_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1795525.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(18239..18757)
                     /gene="msrA"
                     /locus_tag="U3995_000174"
     CDS             complement(18239..18757)
                     /gene="msrA"
                     /locus_tag="U3995_000174"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1795526.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(18777..19490)
                     /locus_tag="U3995_000175"
     CDS             complement(18777..19490)
                     /locus_tag="U3995_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1795527.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(19493..20389)
                     /locus_tag="U3995_000176"
     CDS             complement(19493..20389)
                     /locus_tag="U3995_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1795528.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(20547..21389)
                     /locus_tag="U3995_000177"
     CDS             complement(20547..21389)
                     /locus_tag="U3995_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1795529.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            21521..22174
                     /locus_tag="U3995_000178"
     CDS             21521..22174
                     /locus_tag="U3995_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1795530.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(22237..22764)
                     /locus_tag="U3995_000179"
     CDS             complement(22237..22764)
                     /locus_tag="U3995_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1795531.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(22784..23731)
                     /locus_tag="U3995_000180"
     CDS             complement(22784..23731)
                     /locus_tag="U3995_000180"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1795532.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(23743..25629)
                     /locus_tag="U3995_000181"
     CDS             complement(23743..25629)
                     /locus_tag="U3995_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1795533.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(25789..27867)
                     /locus_tag="U3995_000182"
     CDS             complement(25789..27867)
                     /locus_tag="U3995_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1795534.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(27880..28155)
                     /locus_tag="U3995_000183"
     CDS             complement(27880..28155)
                     /locus_tag="U3995_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795535.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(28517..28972)
                     /locus_tag="U3995_000184"
     CDS             complement(28517..28972)
                     /locus_tag="U3995_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795536.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(29126..30334)
                     /locus_tag="U3995_000185"
     CDS             complement(29126..30334)
                     /locus_tag="U3995_000185"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1795537.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(30446..30778)
                     /locus_tag="U3995_000186"
     CDS             complement(30446..30778)
                     /locus_tag="U3995_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1795538.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            30926..31801
                     /locus_tag="U3995_000187"
     CDS             30926..31801
                     /locus_tag="U3995_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1795539.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(31883..32464)
                     /locus_tag="U3995_000188"
     CDS             complement(31883..32464)
                     /locus_tag="U3995_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1795540.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(32470..33729)
                     /locus_tag="U3995_000189"
     CDS             complement(32470..33729)
                     /locus_tag="U3995_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1795541.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(33731..34777)
                     /locus_tag="U3995_000190"
     CDS             complement(33731..34777)
                     /locus_tag="U3995_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795542.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(35012..35287)
                     /locus_tag="U3995_000191"
     CDS             complement(35012..35287)
                     /locus_tag="U3995_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1795543.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(35530..36840)
                     /gene="der"
                     /locus_tag="U3995_000192"
     CDS             complement(35530..36840)
                     /gene="der"
                     /locus_tag="U3995_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1795544.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(37022..38251)
                     /gene="rpsA"
                     /locus_tag="U3995_000193"
     CDS             complement(37022..38251)
                     /gene="rpsA"
                     /locus_tag="U3995_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1795545.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(38386..39066)
                     /locus_tag="U3995_000194"
     CDS             complement(38386..39066)
                     /locus_tag="U3995_000194"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1795546.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(39115..39750)
                     /locus_tag="U3995_000195"
     CDS             complement(39115..39750)
                     /locus_tag="U3995_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1795547.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(39816..41219)
                     /locus_tag="U3995_000196"
     CDS             complement(39816..41219)
                     /locus_tag="U3995_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1795548.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(41206..42237)
                     /locus_tag="U3995_000197"
     CDS             complement(41206..42237)
                     /locus_tag="U3995_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1795549.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            42268..42489
                     /locus_tag="U3995_000198"
     CDS             42268..42489
                     /locus_tag="U3995_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1795550.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(42654..43391)
                     /locus_tag="U3995_000199"
     CDS             complement(42654..43391)
                     /locus_tag="U3995_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1795551.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(43544..44884)
                     /locus_tag="U3995_000200"
     CDS             complement(43544..44884)
                     /locus_tag="U3995_000200"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1795552.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(45121..46644)
                     /locus_tag="U3995_000201"
     CDS             complement(45121..46644)
                     /locus_tag="U3995_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1795553.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(47022..47633)
                     /locus_tag="U3995_000202"
     CDS             complement(47022..47633)
                     /locus_tag="U3995_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1795554.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(47756..47881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(47963..48679)
                     /locus_tag="U3995_000203"
     CDS             complement(47963..48679)
                     /locus_tag="U3995_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1795555.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(48685..49254)
                     /gene="scpB"
                     /locus_tag="U3995_000204"
     CDS             complement(48685..49254)
                     /gene="scpB"
                     /locus_tag="U3995_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1795556.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(49238..50035)
                     /locus_tag="U3995_000205"
     CDS             complement(49238..50035)
                     /locus_tag="U3995_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1795557.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(50005..50376)
                     /locus_tag="U3995_000206"
     CDS             complement(50005..50376)
                     /locus_tag="U3995_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1795558.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(50395..51282)
                     /gene="xerD"
                     /locus_tag="U3995_000207"
     CDS             complement(50395..51282)
                     /gene="xerD"
                     /locus_tag="U3995_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1795559.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(51378..51851)
                     /locus_tag="U3995_000208"
     CDS             complement(51378..51851)
                     /locus_tag="U3995_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1795560.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(51956..52819)
                     /locus_tag="U3995_000209"
     CDS             complement(51956..52819)
                     /locus_tag="U3995_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1795561.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(53015..55114)
                     /locus_tag="U3995_000210"
     CDS             complement(53015..55114)
                     /locus_tag="U3995_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1795562.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(55334..56443)
                     /gene="rpoD"
                     /locus_tag="U3995_000211"
     CDS             complement(55334..56443)
                     /gene="rpoD"
                     /locus_tag="U3995_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1795563.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(56467..58308)
                     /locus_tag="U3995_000212"
     CDS             complement(56467..58308)
                     /locus_tag="U3995_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1795564.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(58490..60805)
                     /locus_tag="U3995_000213"
     CDS             complement(58490..60805)
                     /locus_tag="U3995_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1795565.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(60990..61850)
                     /locus_tag="U3995_000214"
     CDS             complement(60990..61850)
                     /locus_tag="U3995_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1795566.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
BASE COUNT        16842 a        13250 c        10555 g        21322 t
ORIGIN      
        1 gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt gttcttctgt aaattgaaag
       61 acgtcgacta aattcttttt ggcgtctttt ttgtctttac tttcacgaat cgttgcgatt
      121 acttcatcca gaatcgataa agctttcatc aatccttcta cgatatgctg tcttttttga
      181 gctttggcta aatcgaattg actacgtttt gttatgactt gctttcgatg ctcgatataa
      241 cttcttaaaa tgtctttcag tccaacttga tgcggagtca tatggtcgat agcgaccata
      301 ttgaaattgt aattgatctg tagctcagtg tttttgaaca aataattcaa aattccttga
      361 gcattggcgt cttttttcaa ttcgacgaca atttgcagac ctgttctatc actttcatct
      421 cgtacttcag ctattccatc gattttctta ttcaaacgga tctcatccat ttttttcacc
      481 aaggtcgctt tatttacttc atatgggatt tctgtgatca cgatttgctg tttattaccc
      541 ttcattgcct ctatctttgt tttcgaacga agaataactt ttcctcgtcc tgtttcataa
      601 gcttttttga tttcatcttt tccttgcaag atccctcctg ttggaaagtc aggtccagga
      661 atatattcca tcaatttttc aaggctcgct tgtggatgat cgatcatata gattgtgcca
      721 tcgatcactt cagctaagtt atgagtcggg atttctgttg catatccagc agatatacct
      781 gtcgatccat tgacgagaag gtttgggtat cgagctggaa ggactgttgg ttctttttct
      841 gtatcatcaa aattccacac gagatcgact gtttctttct cgatatctgc tagcatctct
      901 ccacttaact tggataatct ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc
      961 atactcccgt tattcccgtg catttcgatc agtacttccc gcaacttcca gtcttggctc
     1021 atgcggacca tcgcttcata aatactgatg tcaccatgag gatgataatt ccccatgata
     1081 tttccgacag attttgctga tttacggaat cctttgtcat acgtattgcc atctttgttc
     1141 atggcaaata atatccggcg ttgcactggc ttcaatccat cacggatatc aggcagtgca
     1201 cgttcttgga taatatattt ggaataacgt ccgaagcggt cacccatcac ttcttctaaa
     1261 gttaattctt gtacttcttg tcgattttcc atcaggtgac ctcctattct acttcaaata
     1321 actgattgtt ttcattttta tctgctcgtt cttcatctaa taaatcatta gaaactgaag
     1381 gagagatttc agtatcctct tttttatcta agatactgcc atcttcttcg agcgtaaatt
     1441 gcacgtgatt ttcgatccat tttcttcgag gttctacttt gtcacccatc agtgtggtta
     1501 ctcgacgttc agcttgcgca gcatcatcga tacggacgcg gatcaacgta cgggaagtcg
     1561 gatccatcgt tgtttcccac aattgttccg cattcatctc tccaagtcct ttatatcgct
     1621 gcagcatata tcctttgccg acttttttaa tcattgctgc caattcgtca tctgtccacg
     1681 catattcaat gacttgcttc tttccttgtc ctttggatac tttatataaa ggcggtaacg
     1741 cgatatatac tttcccagct tcaatcagcg gtttcatata acgataaaag aaagtaagca
     1801 gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt catgatgatt actttgtcgt
     1861 aattacagtc ttcgatagaa aactcaggac cgactcctgc accaatcgta tagatcatcg
     1921 tattgatttc ttcatttttc aagatatctt gcattttagc tttttcggta ttgatgactt
     1981 ttcctcgtaa aggtagaatt gcttggaact ttctgtctct tccttgttta gcagatccgc
     2041 cagcagaatc cccttcgact aaatacaatt catttttctt agggtttcgg gattgtgcgg
     2101 gtgtcaattt tcctgaaaga agagattcgc cttttttacg cttcttccca tttcggcttt
     2161 cttctctggc tttacgtgct gcttctctcg cttctctggc tttgattgct ttacggacca
     2221 acatctggct catttcgcta ttttcctgca aataaaagcc catttgttca ctgatgacgt
     2281 tatctactac ggtacgagca actggcgtac caagtttttc ttttgtttgc ccttcaaatt
     2341 gcagaagatt ttcaggtaca cggatagaaa gtacagcagc taacccttct ctgaaatcac
     2401 taccttccag attcttgtcc cgttctttca gcaaaccaac ttttctcgca tattcattat
     2461 aagatttcgt catcgccgct ttcataccag cttcatgggt tcccccatct tttgtccgaa
     2521 cgttattgac aaaagataaa acattttctg aataaccgtc attgtattga taagcgacct
     2581 ctacttcgat accttctttt tctcctgaaa aataaacaac tggtgtcaat gtatcttttt
     2641 cttcattcag ataatcaaca aattctttga ttccttcttc gtaatgaaaa atttccttga
     2701 ctggttcttc tccacgaaga tcagaaagag agattttaac gccttttaga agaaaggcag
     2761 attctcttaa ccgttcggct aaaatttcat atgaaaattt cgttgtactg aatattgtat
     2821 catctggtaa aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct atctttttca
     2881 aagtaccgtc aggtttaccg ccgtttttaa atttctgctg atactctacg ccatctctaa
     2941 cgatcgttac aattagccat ttcgataagg catttaccac actggctcct acaccgtgca
     3001 gtccaccaga agttttgtac cctccttgac caaactttcc gccagcatgc agcacagtaa
     3061 aaatcacttc aactgttggt attccggaag catgcattcc tacaggcatc ccacgtccgc
     3121 tatctgcaac agttatacta ttgtcttcat ggatggtcac atcaatctca ctgccataac
     3181 ctgataatgc ttcgtcaact gcattatcga cgatttcata gactaaatga tgcaagcctc
     3241 ggctgtcagt ggatccaatg tacatccctg gtctttttct aactgcctct aatccttcca
     3301 atacttggat ggacgagtca ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc
     3361 cttcaataat actttgcggc aaaaccgcac cacgattatc atactagaaa aggtcgtgaa
     3421 aaaaaagagg gtagacgaga aatcaatttt aattatctcc taaccgcttt ttcgacattc
     3481 ccctctatcg atcatctctt ctttgtggtc atttttattt ttggttttct ttcaacttac
     3541 caagttcaat cttaatacaa cgatccatta cgatatctgt acgtcctgct tttcttagca
     3601 tctcagctgc ttctttgttt tcaatcccta attgtgccca gaacacttta gcatcagttt
     3661 tcaaaaaatc acctgctatt tcaggtaaaa attcactccg tctaaataca tctacgatat
     3721 cgattggatc atcgatttct gtcaaattcg catagacttt ctcgcctaga atttcttcac
     3781 cagccaataa tggatttact ggtatgattt tatatccatt gtcttgcaat acctgtgcaa
     3841 ccatgtaact tgttcgttct ggtttattgc tcaagcctac gacggcaata ttttttgctt
     3901 cttccaaata gttgaaaatc gtttcttgtg ttggattttt tacagacatt tctgccaccc
     3961 cttgcttttc tttttattcc atctattata cttgtatctt tcattttttc aattgttcct
     4021 gttacctatt catgctaaaa taaaggatag ttttcaactt gaaagagggt aaaaaatgaa
     4081 attgatctta ttacttattt tagcatatct tttaggttct attccttccg gtgtctggat
     4141 tggcaaattg tttttcaaaa aagatatccg acaacacggg agcggtaata ctggaacgac
     4201 caatacattt cgtgtattag gcaaaacagc cgggattgtt gtcctgctta tggatatttt
     4261 aaaaggtaca ttggctactt gtttgccgat gatcttccac ctttctggga ttaaccctct
     4321 ttggtttggg gtatgcgcca ttcttggcca tacatttccg atttttgctg ggttcaaagg
     4381 aggaaaagct gttgcgacaa gtgcaggaat gcttttagga tttaatcctg tattttttgt
     4441 ttattcttgt atcatcttta tcgttagttt attttgtaca agtatggtga gtttgagcag
     4501 tatgatcagt gcagtattga tcactttatc tacgatcgtc cttccttatg tagctcctgt
     4561 gattttggct aagcccaatt ggttactgac gatcattgcc tttttagttt cttcctttat
     4621 cttttatcgg cataaagaca atatcaagcg tatcagaaac catactgaaa gtcgaatccc
     4681 gtttggtttg aatagcagca agaaaaaatc ctgatacttt ttcattccaa agagataaaa
     4741 cgggagtgtg acaaatcgat gtcacactcc cttattactt acaggatagc ttttttacac
     4801 accttggtaa acggtgttcc ctgctaactt ctcggtcaca acctcttact tcactgtgat
     4861 ggaatatttt gtcttgaaga tttcatgggc acctagttga ttgatcccta ctttttccat
     4921 caagtttcca ctactgttca ccgtgtcagc gattccgcac cacggttcga tacaaacgaa
     4981 aggcgcttct tttggataag gagaccagat ccctacataa ggcatatctt tatagcttaa
     5041 agttacgcta tgggaagatt tttcacttcg aatggcgtac gcatttagac cccttgtttc
     5101 aaaaaccaag gcatcttgct caaaaagttc acgattcaaa gccagattcg tatttgtctg
     5161 acctaacgtc ttttgatcca tatcgatgaa tgggcctttc aatggtaggc gtaatcttga
     5221 tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac aatccttctt caagaggtac
     5281 attgaaagca ggatgtccac cgatagaaaa gaacatttct ttttcaccgg tatttgccac
     5341 ttgataatga accgtgatgc cgtctcctcc taattcatag ctgatcacta attcaaaatc
     5401 aaatggatac ttttctaatg tctccggcgt actccttaag atgaaactag ccatttcttg
     5461 tccatgttca atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc ccatttgata
     5521 tgttttccct tgataaacat actgatcatc ttttaaacgt ccaacaaaag ggaataaaac
     5581 cggtgcatgt ctgccccaat acgctggatc accttgccag atatactcta aaccatgttc
     5641 ttttgatttc agactgatca attccgcacc gtgttcagcg atttcggcga tcagctggtc
     5701 attttgaata ataacagtca ttgccattcc tccaacttcg acttactttt taaaatggga
     5761 aggctggaac aaacatttct atctgcccca gtccccttac ttctaataac tgtgttttgc
     5821 taactcgtct ttaaaacggg aattcaatac ttttaaataa gttcccttca tgcctaacga
     5881 acgagattca atgattccag ctgattccaa ttttcgtaaa gcattgacga tcacagaacg
     5941 agtgatgccg atactatcgg caatattaga agcagtcaga cgaccttcat ctccatccaa
     6001 tgcttcaaag atagcttgta cagccttcaa ttcgctataa gataatgtgt tgattgccat
     6061 ttggacagcc gttgcactcc gtacatctgc ttcgatgctt cgagacttct gataaaggat
     6121 ctgcataccg acaaccgtag ctccatattc tgctaagact agatcgtcat ctgcaaacga
     6181 ttgttccata cgagataaga taatcgtacc caaacgttcg ccagctccaa agatcggaac
     6241 gacagtcgtc agaccaaatg gatatttctc tcttgactct accggaaatg cagtaatatc
     6301 actagtaatt gggatattag cttcagtttt catcaactgt gccactgtat cagtataaga
     6361 ttcaggaaat tgtttctcaa caaacattgt tttgattcgt tcattgttca catcgtgacg
     6421 ttcattataa cctagtaata ctccgtcact tcctatgata taggtgttgc tgtcaagaat
     6481 accacctagt gtgacagcca ttttatcgta aggcaaatct gcttgcatgt caaaagtatt
     6541 cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt
     6601 cctccgattt tttataaaat atagcgactt aaatcttcat ttgccgcaat tgagccaagt
     6661 ttgtcatcca catatgcttc tgtgatcgtt atttctccca tttgcatatc tggtgcttca
     6721 aataatagat cttctagtag tttttccaaa atggtatgca gtctgcgtgc gccgatatta
     6781 tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta
     6841 aacgtaactg acacattttc tgtaccgatc aacgcgacgt attgttttac taaagcatta
     6901 tttggttcgg tcagaatctt cacaaaatct tcagctgtca aatcatctaa ttctacccgt
     6961 atcgggaagc gtccttgaag ttctggaatt agatcacttg gtttagacaa atggaaagct
     7021 cctgaagcaa tgaataggac gtgatctgtt tggatgatcc cgtattttgt gtttacttgt
     7081 gaaccttcga caattggcaa tatgtctctt tggacgcctt ctctggatac ttctccagat
     7141 tgctgtgatt tagaagtgat cttatcgatt tcatcgataa aaataattcc actttcttcc
     7201 gctaatttga ttgcctcact atggatatca gcatccttga ctaattttgc tgattcttcc
     7261 ttgatcaata actctaaagc ttctttgact gttaccgtcc gttccacttt tttcttcggt
     7321 gtcaaagcac ctagagtttc atttaggtcg atccccattt gctccaatcc attgttcatc
     7381 attggtgcag gtttcttttg gtcttcaact tcaatggtta cttcacgact atccagtaaa
     7441 cccttttgta gttgttccaa gactgtacgg cgattgattc gaatatcttc tgtcacttct
     7501 tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa gattcatcat ttgttcaaat
     7561 tgattagtgg attgtttctg ttcttttttg ataccaggta ccaagatttt taccaaacgt
     7621 ttttctgcat tttttttagc acttgtgtat acacggctgt actgctcttt ttctacgatt
     7681 tggatcgcat tttctactaa atcccgaacc attgattcta catctcttcc gacataaccg
     7741 acttcagtaa attttgttgc ttcgactttg acaaatggtg cattgacgat ttttgccaaa
     7801 cgtcgtgcga tttctgtttt accgacccct gtaggtccga tcatcagcat attttttggt
     7861 gtaatatctt gttgcatttt ttcatctaac tgcaaacggc gatagcggtt acgcaaagca
     7921 acggcaacag atttttttgc tgcgtgctgt cctacgatat attcatctaa ttcacttacg
     7981 atttgtcttg gtgtttgttt taattcatac atatctactc acctcttaca gttcttctac
     8041 gataatatta tgattagtaa agatacagat atctgcggca atatttaacg cattttctgc
     8101 aatttctttc gcactcatgt cgttttgagc atagtttttc attgctcggg ctgcagccaa
     8161 cgcaaaattc cctcctgatc cgatagctaa gataccatca tctggtgtga taacttctcc
     8221 tgtacctgaa acaagaagca tttctttatc attcattacg atcagcatcg cttcaagttt
     8281 ttgcatagat tgctgtgtcc gccattcttg agctaattcg acagctgcgc gttgtagatt
     8341 cccgttatat tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg catctgcaac
     8401 acttccggcg aaacctacaa caacttctcc attgtagata cgtcgtactt tctttgcagt
     8461 gcctttcatg acaacagatt cacccattgt tacttggcca tctccagcca tagcaaattt
     8521 cccgtctttt tcgacggcac agatagtggt tgaatgaaat ggtgattcga ccatgtttgt
     8581 ttcctcctaa atttgttctt ttccttattt ttacgctctt ggatggaaag aacgatagtt
     8641 tttttgtaaa ctttcttttg ttacatgggc atagatctgg gtagtcgata aattggcatg
     8701 tcctagcaat tcttggactg tccgcaaatc tgccccatta tttaataaat gagtagcaaa
     8761 tgtatggcgc agcatgtgag gatggatatc actatccaaa ctgctttttt tgatcatttg
     8821 attcaagaca tattcaattc cagtaggcgt gattggtttt ccatgatgat tcacaaaaac
     8881 gatatcatgc tcttcgtgat attttgtcat caatacctgg cggccttccc tgaaatattc
     8941 ttgcagagca tcttgagcaa aagaacctag aggagcatat ctctctttgt tccctttacc
     9001 atggatcaac atgacctctg aatcaaaatc aatgtctgat accgacaaac tgctacactc
     9061 acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa
     9121 aggactatct cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg
     9181 tggcagtctc aaatttttct ttttgagatg aacgtaagaa aatggatttt cttttatcac
     9241 ttcttctttg agaagatact gataaaacga acgtaggctg gctatcttgc gactgataga
     9301 atttctgcta tagctaggac gatgatcagc taatgcactc aaatagacac gaaccgtaaa
     9361 atattccgcc ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag
     9421 aatgtcttct tcatatgctt ctttcgtttt ttctgaatat cctctttcga caatcaaata
     9481 acgcaaaaat ttttgttccc acgtggcttc tgtcattttc tcaccttcct tttgtcccag
     9541 agaataattt agcacagcgt ttataaaaag acaaataaat tgtcagaatt taaccaatat
     9601 ttctaagttt gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa
     9661 aaacgcaaga aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca
     9721 tttgggattg cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt
     9781 tgtgcaattg cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac
     9841 agaccatagt tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa
     9901 gccatacttc caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag
     9961 cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca
    10021 ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc
    10081 agcaattctg gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca
    10141 gcattttcta acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga
    10201 gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata
    10261 acagcataag gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg
    10321 aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa
    10381 tatttttctt tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg
    10441 aaagctttga attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca
    10501 tagcgggact tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca
    10561 gctgcatcat agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat
    10621 ggatgagaag tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt
    10681 tcttcgttta tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa
    10741 aaagaatcgc ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa
    10801 gtgattacta ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc
    10861 aaactgtttc ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc
    10921 gcctcagtcg atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg
    10981 atctgccaag cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat
    11041 gaaagaacct cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac
    11101 tacttgttta ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt
    11161 tctgcccact ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata
    11221 gaatagtctg tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca
    11281 agtcacaccg atttctttta cgattggttt cgtattccga caatcaggaa aattactgca
    11341 agcataaaac ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca
    11401 atcgaatcct gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt
    11461 cagttctttt tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc
    11521 ttcttcaatt ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg
    11581 agggaagaat tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct
    11641 tttttgtgtc aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt
    11701 ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg
    11761 tgctggtggt tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc
    11821 ttcttcaagt tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac
    11881 atacacttgc atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc
    11941 attttgttct aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac
    12001 aaaacgtgac cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag
    12061 ttcatctggt gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc
    12121 ttgagcattt ttgatctttc ggtgatcatg tctagaaaat tcttttccat acgtatcttc
    12181 aataaatgct gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat
    12241 gtatgtaatc aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg
    12301 tgctaccatc atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag
    12361 gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt
    12421 aacttcgaag tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg
    12481 taatttcttt ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt
    12541 tttaaaatta ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc
    12601 acggtcaata ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt
    12661 gaccttacgc cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg
    12721 agcttgctgt gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt
    12781 gactgcgtct tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc
    12841 aagtaggtac gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa
    12901 gtaaactttt tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc
    12961 gcgtatagaa atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt
    13021 cggtaaatct cttatatggc caacactggc cattacttta tagtttcgtc caagatattt
    13081 ctcaattgtc ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac
    13141 tgaatggact cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt
    13201 agctaatcat atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt
    13261 attctttaaa ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga
    13321 tcaaatgatg acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg
    13381 ttcttccaag atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt
    13441 caaagacaca tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat
    13501 gctttttcgg gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca
    13561 cctcttgcaa gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg
    13621 cgattgtccg tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg
    13681 cgcagccact gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg
    13741 tttttttggc taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa
    13801 gtaaagatag atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct
    13861 gagggggata tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc
    13921 agttagcgtg aatttcctgc caacttttgc gaaacagctc ccggtgtttg gttattttac
    13981 ctatcttaat gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag
    14041 caagtgccca ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt
    14101 cttctatttg atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa
    14161 gcggctgttt tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc
    14221 gtaattcctt gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc
    14281 gagaaaccat atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact
    14341 ttagcaatga tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt
    14401 tcctgctgaa taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg
    14461 gacaattttt ctactgcttg gatcatcgct aatttacttg cttcataaat attcacatca
    14521 tcgattgtct gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt
    14581 tgataaagaa gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg
    14641 atctcatttt tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt
    14701 cctacttcat caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag
    14761 caaaacattt cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa
    14821 gaagccagtg cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga
    14881 tcatctattc tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc
    14941 acttttttgc tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg
    15001 aatatcatgc acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc
    15061 tgtaatatcc atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata
    15121 acgatcgctg attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa
    15181 atcatctaaa tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat
    15241 ttcttcatct tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt
    15301 acctaaacga agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat
    15361 tttcttgcct actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac
    15421 cattgcccga atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt
    15481 caatgcttct ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat
    15541 agctaatgca agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg
    15601 atctgccaga tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc
    15661 tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc
    15721 cacatatttc attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca
    15781 ttggatggtc attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt
    15841 ttttttcttt ttcaatataa attcactttt ttattataaa gaaattccat ctattagtta
    15901 agtgaaagtg aaccagtcct cattctatca catcttcact tttttgatga tgcaaatttt
    15961 tattttggtt tctatatatg aatctaaatt tttgacagcg tttcctaaat aagagatgat
    16021 agacgaacaa aacagaaagg agacgaaaca aatgaatagt atggtttttg ttaattttcc
    16081 tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta aaaaaaatga
    16141 agaattttct actgaagaag ctggttcaat ggtatgggat gacacttttt ggataatgct
    16201 tctctctcat gatttttata aaaaatttat aaaagataaa gaaatagctg ataataaaaa
    16261 aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa aaaaatttgc
    16321 tcaaattgca aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga
    16381 agaaatgttt tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat
    16441 gaagatgtaa gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga
    16501 tacgataagt attctccgcc tctatttttt ttattcgtac aatctctttt ttacagttta
    16561 taaaagcagt cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa
    16621 tgtgtcatga cacattggtg attttatggt atccttttat tattaactga caaaggagag
    16681 aatattttga acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga
    16741 ttgttgtcga cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt
    16801 ggtcctgctt attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta
    16861 tatggaccga tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata
    16921 tttggttcag gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt
    16981 aatggcttat ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta
    17041 ttgattatga tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt
    17101 gcagatttca aagtaatttt tctaggtaga ttcatcagga atatgatcca aatccctatt
    17161 caaataatgg ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga
    17221 gctgtttgat tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc
    17281 agtctcaaca gactgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg
    17341 gataatagac aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac
    17401 gactatcttt tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct
    17461 tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat
    17521 ctgatttcag gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt
    17581 gatcattttg gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg
    17641 tgccatctgc taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca
    17701 tcacaaccat atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg
    17761 ggatgaataa aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga
    17821 atacaagcca gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc
    17881 ctcttttttt cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc
    17941 tgaaataaaa ataattcttt caattaggct attggacaaa aaactttttt gatcataaag
    18001 tacaaaacta tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca
    18061 aagatatcca tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg
    18121 tagctagagc tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat
    18181 aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc
    18241 acccttcgtt cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa
    18301 aattttcagg atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt
    18361 gggctggttc gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt
    18421 tcttactttt ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat
    18481 aattgtcacc gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt
    18541 aaagttctac taattgttca tatgaaatca atgttggatc atagataatc tccactgctt
    18601 ctgtatgtcc agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc
    18661 cagtatatcc tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac
    18721 accagaagca gcctcctgca aatattgctc gttccattat gattccccct ctaatactac
    18781 tcatccgatg attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg
    18841 ttggtatttg agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga
    18901 atgtcatcat ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg
    18961 aattgatcca acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt
    19021 aatttatagt ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg
    19081 atagaaagac ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa
    19141 tataaataga aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca
    19201 ttttccaaat aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag
    19261 ctgatgattt gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt
    19321 ctttccgtaa gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg
    19381 cctaaaaata cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag
    19441 taatttgttt tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat
    19501 catcctcctt ctctaaccat tggttttgtg tctcgatcag ctgatctctc actgcatttg
    19561 ccatcaattg ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat
    19621 ttttgatgcc atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga
    19681 cacctacttc ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt
    19741 tcggcatttt ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca
    19801 tgtcagtgat ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag
    19861 gtgcggtttt attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt
    19921 ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt
    19981 tcatcaaatc attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc
    20041 gaatttcttc ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac
    20101 catagttttc tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa
    20161 ggggaacgaa tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga
    20221 tagctatgta gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt
    20281 tcattagtgg gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa
    20341 ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt ttttcacctc
    20401 ttgtattcga ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa
    20461 aaagaaaaca attattttcc taattttata aagaaaaaga gaataagctg caagcctttc
    20521 aggctgaggg cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct
    20581 ttaccagtat gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg
    20641 aataattctt gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa
    20701 ataccgattt gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat
    20761 ttatcgaacg ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa
    20821 ttcatcacca cgcgtatgtt caaaacacta gacagcaaac cagttgttcg gctgattctt
    20881 ccacctttta ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt
    20941 acttttttga cttcttcaag gattttctct actgttttac cttcttttgc taattcagct
    21001 gctcgaatta cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact
    21061 ttcgtttttg ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt
    21121 gtcatatgga tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagatg
    21181 aattctccaa ttggtggttg gcttgttttg ggcagatttt ttgctttcgc catcatttcc
    21241 ataaatcttt cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg
    21301 gatagcggca tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa
    21361 gaatctgtta cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg
    21421 ccttattcat gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa
    21481 tgatttttca ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa
    21541 tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg
    21601 attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct
    21661 ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac
    21721 tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag
    21781 ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg
    21841 atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata
    21901 taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat
    21961 ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac
    22021 tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag
    22081 cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg
    22141 ttttatgtac ttctctgttt tattctttac ttaataaaaa aaataaagct atggatggga
    22201 cagctctaaa taacagctag tctatccata gcttttttat atcgtcaatt cgcctgtccc
    22261 gctttttaca ggattctcat tacgctgata cgtttcaaat tgatgcggat aacgattttt
    22321 ttcatccatt tctccttgac tagtattgat cagtgaccaa tcagaccagt caacaggtgg
    22381 aaaataagca tctccatcga atacatgatg gatgactgta cggtataata tcgtacaaaa
    22441 tggcagaaat tcttgataaa cagctgaccc tccagcgatg aaaaagatac cgtctgtttc
    22501 tttttccaga gcaagcacgt cttccacact atgcatgaca tgcacgcctt ctgctttata
    22561 ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc aacggacggc tacccatccc
    22621 ttcgaatgtt tttcgtccca tcactagtgt atttgcttct gtcatttgtt tgaagaattt
    22681 cagatcattt ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc cgttttcatc
    22741 ctgtgcccaa attgcagcta acattttctc atcctttctg attctaaaca gcgataggag
    22801 ctttgatcgc cggatgtggg tcatatcctt ctaattggat atcttccatc tcaaaatcaa
    22861 atactgactg cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca
    22921 attgttcttt catttgttcg atatgattga tataaagatg agcatctcct aatgtgtgaa
    22981 caaattcgcc aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc aacgcataac
    23041 ttgcaatgtt gaacggaacg cctagaaaca catccccact gcgttgatac aattggcagc
    23101 ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg acaaggaggc aaagccatgc
    23161 taggaacatc ttccggattc catgcagaaa cgatcaatct tctagaatca ggagtctttt
    23221 tgattgcttc gatcacttgt ttgatttgat cgataaagcc accgtctttt gtttcccagt
    23281 gacgccattg atagccgtag atgtgtccaa gatcgccgta tgtttcagca aaggaatcat
    23341 cggttaggat cttctcgcaa aaaagcttgc tctgcttttt atattcttca ttgaattcag
    23401 gatctgtcaa acatctatgg ccaaaatcag tcatatctgg tccattatag tctggacttt
    23461 tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt tttattttgt aatagatagc
    23521 ggatattcgt atctcctttt aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta
    23581 ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt tggtatccga
    23641 ataagcttaa tgtgcctgtg cctgttctgt cttctttacg atgcccttct tccaacaatt
    23701 ttcttcctaa gtcaagatac gcttgttcca tcctttttcc tcctaatctt ctacccattc
    23761 actcaagtat tcccaccgtg ccattttttc ttcaagttgt gcttctgttt cagatacgtc
    23821 gttttgcaat tcttgtaatc gagtgaaatt atctccttga tgattcatct cttcttggag
    23881 caatgagatt ttttcttcaa gttctgcaat ctcatcttca atcgtttccc attctttttg
    23941 ttccatgtaa gataatttct tcttttcagc aggttccttg ctctgtattt ttggtttcgc
    24001 tttttcagca ggctgctggg tctctttttg cttcagtaaa taactggaca tgtttccgta
    24061 ataaagttct tgttttccgt ttccttggaa tactaataac ttatctgcta ccttgtccaa
    24121 gaaataacgg tcatgggaaa cagtgatcac agcacctgga aaacttctaa aataatcttc
    24181 caaaatcgtc aatgttgcta tgtccaaatc attggtcggt tcatccaata ataaaacatt
    24241 aggttgctga atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt
    24301 gcctatcagt gtcccatgca taaatcttgg aaataaaaat cgttcaagta attctgctac
    24361 tccgatttga ctgccatctg ctgtttttgc ttcttcagca gcttcttgta agtaagcaat
    24421 catccgttta tctggtgcca tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt
    24481 ttcaccgata gacattgtac cgctatccag gggaattctg ccagctagaa tattaagcaa
    24541 tgtcgatttt cccgcaccat ttttaccagt aatacctagt ctttcccgag attgaatcaa
    24601 taaatctaaa tgttctagca gtgtttggtc atttatacgg taactgccat ctttgatttc
    24661 cagtactttt ttccctaaac gttgtgtcgc taggttcagc tctaaatcat cttcttggtt
    24721 tacttggtac aagttctcct ttagatcttc gaaacggttg atccgtgctt gctgcttggt
    24781 gccacgtgcc tgtacgccag ccctcatcca agctaattcc tgtttataaa gccgcttgcg
    24841 cttttcttct tgttctactt ctacacgatc acgttctgct ttttctaaga cgtaagcttc
    24901 ataatttcct ttatactcgt ataattttcc aaatgaaagt tcgaagattc ggtttgctac
    24961 tctatctaga aaataacgat cgtgggtaac catcaatagt gcgccacggt attgtttcaa
    25021 atagttttcc aaccactcga tcgcttcata atctaaatgg tttgttggtt cgtctagtaa
    25081 tagtaaatcg ggttcatcaa tcaacacttg cgccagacta actcgttttt tctgaccacc
    25141 tgataaagta ctgatttttt tatctaattc actgattcct aatttttgca aaataatctt
    25201 tgcgttggta tctgtagtcc aagcatcttc tttattcatt tgttcttctg ctttagcata
    25261 acgtttttgc acatcagggt cttctccgtt ttcccctagt tcgatcaatg cttcctcata
    25321 tgctcgaaca gtttggatca aggggctgtt tccttgaaag actgcttgga gaacagttaa
    25381 ttcatcggaa aattcagctg cttgagaaag ataaccaata cggtaatcag ttggataaaa
    25441 cactgtttgc cgatcaccgt cgctgctatc gataccggca ataatattta agagacttgt
    25501 tttacccgtc ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga
    25561 aatctggtca aacaaatctt tttcaccata agtttttttg aggtcagtta cttttaattc
    25621 tttcaacatg ttccctcctt atcttggata ttgtatcata aaaatagtga acttcaggaa
    25681 aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc
    25741 tgttctgata tgttttgttt tttataaaac ttgaaaattt ttaccttttt attttattgg
    25801 actcgttttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt
    25861 gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt
    25921 cgtgattttt tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct
    25981 tagcattttt ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc
    26041 cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc
    26101 atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata
    26161 tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct
    26221 tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa
    26281 ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa
    26341 acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa
    26401 tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc
    26461 atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc
    26521 ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac
    26581 ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt
    26641 ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc
    26701 cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa
    26761 aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat
    26821 ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa aaagactgat
    26881 agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag
    26941 accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc
    27001 taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga
    27061 caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat
    27121 acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc
    27181 tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt
    27241 ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc
    27301 cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat
    27361 cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc
    27421 tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac
    27481 tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac
    27541 acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc
    27601 aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc
    27661 tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc
    27721 gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg
    27781 agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa
    27841 tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag
    27901 catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt
    27961 ttggttcccc tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt
    28021 cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg
    28081 acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc
    28141 aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt
    28201 ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata
    28261 aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa
    28321 gataaaagtt tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta
    28381 tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa
    28441 cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt
    28501 ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg
    28561 gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat
    28621 tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat
    28681 ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc
    28741 ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga
    28801 tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc
    28861 caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc
    28921 aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga
    28981 ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg
    29041 gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag
    29101 ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa
    29161 cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag
    29221 cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta
    29281 ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca
    29341 tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc
    29401 ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga
    29461 caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac
    29521 gaatctcatt ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt
    29581 ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca
    29641 aaccgggaca gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt
    29701 catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat attttaccta
    29761 atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt
    29821 gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag
    29881 gattgcctac agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga
    29941 tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat
    30001 cttctgccaa cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg
    30061 attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc
    30121 catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg
    30181 cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc
    30241 cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct
    30301 tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct
    30361 tgtcctgaga taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt
    30421 caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc
    30481 tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat
    30541 tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg
    30601 gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac
    30661 ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt
    30721 gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct
    30781 ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga
    30841 gaaaatcgaa tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa
    30901 tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt
    30961 tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca
    31021 tttagcagaa gggggagtct cagggatcac cttgatctta agagctttat ttggtattga
    31081 tccagcttat acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct
    31141 cggtaaacgt tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg
    31201 gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct
    31261 acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac
    31321 aggcggcagt gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg
    31381 ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa
    31441 acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt
    31501 agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc
    31561 accactactg atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt
    31621 ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc
    31681 taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga
    31741 agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga aaaaactata
    31801 agaaaaaaga caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt
    31861 tttagtacta tactattttt aactaattgt tatgttcact tacccggtta cttttctttt
    31921 gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat
    31981 ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt
    32041 ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc
    32101 aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt
    32161 cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct
    32221 ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag
    32281 atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat
    32341 tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc
    32401 taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta
    32461 gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg
    32521 ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct
    32581 taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg
    32641 ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt
    32701 ccattctgca taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc
    32761 atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc
    32821 tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa
    32881 agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc
    32941 ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata
    33001 tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc
    33061 ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt
    33121 cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat
    33181 acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct
    33241 tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa
    33301 ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa
    33361 ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa
    33421 gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc
    33481 gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg
    33541 gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag
    33601 caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa
    33661 aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt
    33721 tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc
    33781 tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa
    33841 caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg
    33901 aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt
    33961 ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta
    34021 aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt
    34081 caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt
    34141 cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat
    34201 acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt
    34261 caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg
    34321 gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg
    34381 ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg
    34441 ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga
    34501 taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg
    34561 ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa
    34621 ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga
    34681 ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt
    34741 tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct cttcttattt
    34801 ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact
    34861 tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg
    34921 gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag
    34981 ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac
    35041 ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt
    35101 tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa
    35161 ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag
    35221 ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt
    35281 ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa
    35341 actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat
    35401 cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt
    35461 agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg
    35521 gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa
    35581 tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc
    35641 attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt
    35701 caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc
    35761 attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc
    35821 aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc
    35881 aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt
    35941 ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta
    36001 acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac
    36061 gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc
    36121 gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact
    36181 ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat
    36241 tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc
    36301 aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt
    36361 cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg
    36421 atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac
    36481 tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc
    36541 atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc
    36601 aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat
    36661 tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg
    36721 atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt
    36781 aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat
    36841 attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt
    36901 caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt
    36961 ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca
    37021 attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta
    37081 aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt
    37141 ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat
    37201 gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt
    37261 catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc
    37321 cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata
    37381 ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga
    37441 ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat
    37501 cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat
    37561 ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa
    37621 cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt
    37681 ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga
    37741 tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat
    37801 gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc
    37861 ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga
    37921 tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt
    37981 ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta
    38041 cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa
    38101 ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct
    38161 taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt
    38221 tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc
    38281 cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt
    38341 tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata
    38401 accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt
    38461 cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc
    38521 ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt
    38581 ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat
    38641 tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc
    38701 accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc
    38761 agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt
    38821 tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt
    38881 gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata
    38941 agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa
    39001 tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg
    39061 tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt
    39121 aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc
    39181 aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc
    39241 ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt
    39301 gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc
    39361 tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat
    39421 tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact
    39481 ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc
    39541 atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa
    39601 aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga
    39661 aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc
    39721 tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta
    39781 ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat
    39841 aaatttagta aaatcgttcc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt
    39901 ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt
    39961 ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga
    40021 agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa
    40081 ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag
    40141 ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa
    40201 agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca
    40261 ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct
    40321 ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat accaaatgca
    40381 ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt
    40441 tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga
    40501 tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt
    40561 tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata
    40621 ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt
    40681 atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct
    40741 aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga
    40801 gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct
    40861 aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga
    40921 tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt
    40981 ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac
    41041 acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc
    41101 gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct
    41161 tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt
    41221 ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa
    41281 ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc
    41341 caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa
    41401 ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat
    41461 ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa
    41521 attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat
    41581 ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt
    41641 gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata attggttagc
    41701 taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc
    41761 agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa
    41821 agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga
    41881 aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg
    41941 gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg
    42001 cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa
    42061 aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc
    42121 gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag
    42181 agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt
    42241 attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc
    42301 tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa
    42361 ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa
    42421 gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat
    42481 aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata
    42541 agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc
    42601 tatctatagt taattgacta gttttttcac ggaccctata ggacccattt atattatttc
    42661 aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa
    42721 atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg
    42781 ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct
    42841 tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct
    42901 gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg
    42961 tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg
    43021 atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc
    43081 actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct
    43141 tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa aataagtaat
    43201 ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt
    43261 ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag
    43321 taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta
    43381 aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat
    43441 gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga
    43501 cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac
    43561 gatacacagc ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt
    43621 cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact
    43681 ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag
    43741 taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata
    43801 atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta
    43861 atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt
    43921 ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt
    43981 catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta
    44041 agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag
    44101 cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt
    44161 caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct
    44221 tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag
    44281 aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa
    44341 ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat
    44401 atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg
    44461 tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag
    44521 gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga
    44581 atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga
    44641 taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt
    44701 taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca
    44761 cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc
    44821 ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt
    44881 tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc
    44941 tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac
    45001 aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg
    45061 atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt
    45121 ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa
    45181 tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat
    45241 tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt
    45301 gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt
    45361 tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa
    45421 tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa
    45481 gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac
    45541 tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga
    45601 ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata acatagtaat
    45661 ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac
    45721 cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt
    45781 tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac
    45841 atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt
    45901 catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga
    45961 tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg
    46021 aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa
    46081 aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc
    46141 tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat
    46201 cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg
    46261 aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag
    46321 tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat
    46381 tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt
    46441 gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga
    46501 tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt
    46561 aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa
    46621 ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat
    46681 tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt
    46741 tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt
    46801 taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc
    46861 ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca
    46921 aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt
    46981 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
    47041 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
    47101 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
    47161 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
    47221 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
    47281 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
    47341 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
    47401 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
    47461 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
    47521 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
    47581 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
    47641 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
    47701 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
    47761 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
    47821 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
    47881 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
    47941 aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
    48001 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
    48061 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
    48121 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
    48181 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
    48241 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
    48301 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
    48361 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
    48421 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
    48481 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
    48541 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
    48601 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
    48661 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
    48721 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
    48781 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
    48841 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
    48901 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
    48961 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
    49021 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
    49081 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
    49141 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
    49201 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
    49261 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
    49321 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
    49381 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
    49441 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
    49501 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
    49561 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
    49621 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
    49681 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
    49741 attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
    49801 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
    49861 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
    49921 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
    49981 taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc
    50041 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
    50101 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
    50161 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
    50221 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
    50281 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
    50341 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
    50401 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
    50461 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
    50521 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
    50581 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
    50641 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
    50701 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
    50761 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
    50821 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
    50881 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
    50941 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
    51001 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
    51061 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
    51121 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
    51181 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
    51241 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
    51301 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
    51361 aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata gcattcctga
    51421 cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact
    51481 acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag
    51541 catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat
    51601 ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga
    51661 taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct
    51721 gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt
    51781 gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca
    51841 ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa
    51901 cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat
    51961 tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa
    52021 gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt
    52081 ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta
    52141 aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca
    52201 gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt
    52261 cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg
    52321 tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg
    52381 ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt
    52441 cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg
    52501 tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga
    52561 caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac
    52621 ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc
    52681 cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc
    52741 cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag
    52801 ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt
    52861 ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg
    52921 aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa
    52981 cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc
    53041 atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt
    53101 tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc ccattttcga
    53161 ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag
    53221 caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa
    53281 tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg
    53341 aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg
    53401 cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg
    53461 cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt
    53521 tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct actgcttctt
    53581 ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta
    53641 tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg
    53701 cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat
    53761 ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc
    53821 actcattcac tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta
    53881 actctgaata tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat
    53941 cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa
    54001 gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt
    54061 ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg
    54121 ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt
    54181 tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg
    54241 cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag
    54301 taccctcttt gcttacagat tgattttcag catcggattc actatttatt tcatcgggct
    54361 ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat
    54421 tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg
    54481 tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt
    54541 tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga
    54601 tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat
    54661 aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc
    54721 ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct
    54781 catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag
    54841 gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta
    54901 aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat
    54961 cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta
    55021 tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca
    55081 tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag
    55141 gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg
    55201 atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt
    55261 agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt
    55321 caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca
    55381 attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt
    55441 tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga
    55501 cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact
    55561 cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt
    55621 gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca
    55681 agatttctcg gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag
    55741 gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt
    55801 caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg
    55861 cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt
    55921 tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc
    55981 ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac
    56041 gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg
    56101 cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt
    56161 ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac
    56221 tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt
    56281 caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct
    56341 gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg
    56401 ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt
    56461 gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc
    56521 aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt
    56581 tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg
    56641 cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc
    56701 atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc
    56761 atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt
    56821 taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt
    56881 ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg
    56941 ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg
    57001 tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc
    57061 ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat
    57121 cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa
    57181 gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag
    57241 atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt
    57301 ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata
    57361 gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact
    57421 tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat
    57481 aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag
    57541 attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg
    57601 ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg
    57661 gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc
    57721 atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt
    57781 cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc
    57841 aattaattct aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt
    57901 tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt
    57961 ccccgtagca gattcaggat ttcggacagc tatagtacgc tgacgccatt gatcttctaa
    58021 cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag
    58081 ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg
    58141 cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa
    58201 gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt
    58261 ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc
    58321 tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa
    58381 atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat
    58441 catatttcat tgctttttca agtataacac atttctatct cattaagcat caatttgcta
    58501 ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga agaacaatat
    58561 ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca
    58621 aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga
    58681 agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa
    58741 tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa
    58801 gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa
    58861 gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga
    58921 aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa
    58981 atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa
    59041 aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt
    59101 ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc
    59161 catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca
    59221 caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt
    59281 tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt
    59341 ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac
    59401 gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac
    59461 cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt
    59521 caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag
    59581 cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa
    59641 gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa
    59701 attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc
    59761 gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt
    59821 ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt
    59881 ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg
    59941 tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg
    60001 ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa
    60061 gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga
    60121 tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt
    60181 tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct
    60241 cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca gccatgacag
    60301 aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat
    60361 cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat
    60421 cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag
    60481 tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg
    60541 ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata acgatattga
    60601 cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc
    60661 caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg
    60721 tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta
    60781 cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa
    60841 taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt
    60901 tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc
    60961 cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc
    61021 caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact
    61081 cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa
    61141 atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat
    61201 ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct
    61261 ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc
    61321 aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa
    61381 ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga
    61441 acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct
    61501 ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt
    61561 tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg
    61621 cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct
    61681 aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact
    61741 cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa catccagtct
    61801 ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgta
    61861 ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac
    61921 tttttcatag gtctctatta ttttttcatt cattttgaca atatcagga
//
LOCUS       DASJPH010000004        59982 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJPH010000004 DASJPH010000000
VERSION     DASJPH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.004"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="U3995_000215"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="U3995_000215"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1795567.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="U3995_000216"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="U3995_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1795568.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="U3995_000217"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="U3995_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1795569.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="U3995_000218"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="U3995_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1795570.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="U3995_000219"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="U3995_000219"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1795571.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="U3995_000220"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="U3995_000220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1795572.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="U3995_000221"
     CDS             complement(8297..8767)
                     /locus_tag="U3995_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1795573.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="U3995_000222"
     CDS             complement(8813..9238)
                     /locus_tag="U3995_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1795574.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="U3995_000223"
     CDS             complement(9305..9574)
                     /locus_tag="U3995_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1795575.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="U3995_000224"
     CDS             complement(9585..11180)
                     /locus_tag="U3995_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1795576.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="U3995_000225"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="U3995_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1795577.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="U3995_000226"
     CDS             complement(14733..15293)
                     /locus_tag="U3995_000226"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1795578.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="U3995_000227"
     CDS             15646..16620
                     /locus_tag="U3995_000227"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1795579.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="U3995_000228"
     CDS             16790..18190
                     /locus_tag="U3995_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1795580.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="U3995_000229"
     CDS             complement(18268..18990)
                     /locus_tag="U3995_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1795581.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="U3995_000230"
     CDS             complement(19069..19575)
                     /locus_tag="U3995_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1795582.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="U3995_000231"
     CDS             19698..21497
                     /locus_tag="U3995_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1795583.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21565..23175)
                     /locus_tag="U3995_000232"
     CDS             complement(21565..23175)
                     /locus_tag="U3995_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1795584.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23430..23939)
                     /gene="mreD"
                     /locus_tag="U3995_000233"
     CDS             complement(23430..23939)
                     /gene="mreD"
                     /locus_tag="U3995_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1795585.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23943..24797)
                     /gene="mreC"
                     /locus_tag="U3995_000234"
     CDS             complement(23943..24797)
                     /gene="mreC"
                     /locus_tag="U3995_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1795586.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24952..25590)
                     /locus_tag="U3995_000235"
     CDS             complement(24952..25590)
                     /locus_tag="U3995_000235"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1795587.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25591..26241)
                     /locus_tag="U3995_000236"
     CDS             complement(25591..26241)
                     /locus_tag="U3995_000236"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1795588.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26254..27153)
                     /gene="rsgA"
                     /locus_tag="U3995_000237"
     CDS             complement(26254..27153)
                     /gene="rsgA"
                     /locus_tag="U3995_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1795589.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27318..29387)
                     /gene="pknB"
                     /locus_tag="U3995_000238"
     CDS             complement(27318..29387)
                     /gene="pknB"
                     /locus_tag="U3995_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1795590.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29384..30124)
                     /locus_tag="U3995_000239"
     CDS             complement(29384..30124)
                     /locus_tag="U3995_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1795591.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30137..31495)
                     /gene="rsmB"
                     /locus_tag="U3995_000240"
     CDS             complement(30137..31495)
                     /gene="rsmB"
                     /locus_tag="U3995_000240"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1795592.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31495..32433)
                     /locus_tag="U3995_000241"
     CDS             complement(31495..32433)
                     /locus_tag="U3995_000241"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1795593.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32426..32917)
                     /gene="def"
                     /locus_tag="U3995_000242"
     CDS             complement(32426..32917)
                     /gene="def"
                     /locus_tag="U3995_000242"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1795594.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32932..35340)
                     /gene="priA"
                     /locus_tag="U3995_000243"
     CDS             complement(32932..35340)
                     /gene="priA"
                     /locus_tag="U3995_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1795595.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35593..35898)
                     /locus_tag="U3995_000244"
     CDS             complement(35593..35898)
                     /locus_tag="U3995_000244"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1795596.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35903..36517)
                     /gene="gmk"
                     /locus_tag="U3995_000245"
     CDS             complement(35903..36517)
                     /gene="gmk"
                     /locus_tag="U3995_000245"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1795597.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37004..37288)
                     /locus_tag="U3995_000246"
     CDS             complement(37004..37288)
                     /locus_tag="U3995_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795598.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37306..38196)
                     /locus_tag="U3995_000247"
     CDS             complement(37306..38196)
                     /locus_tag="U3995_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1795599.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38216..38434)
                     /locus_tag="U3995_000248"
     CDS             complement(38216..38434)
                     /locus_tag="U3995_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1795600.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38404..40104)
                     /locus_tag="U3995_000249"
     CDS             complement(38404..40104)
                     /locus_tag="U3995_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1795601.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40371..41144
                     /locus_tag="U3995_000250"
     CDS             40371..41144
                     /locus_tag="U3995_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795602.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41225..41794)
                     /gene="thiT"
                     /locus_tag="U3995_000251"
     CDS             complement(41225..41794)
                     /gene="thiT"
                     /locus_tag="U3995_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1795603.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41860..41962)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41968..42939)
                     /locus_tag="U3995_000252"
     CDS             complement(41968..42939)
                     /locus_tag="U3995_000252"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1795604.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43033..44406)
                     /gene="glmU"
                     /locus_tag="U3995_000253"
     CDS             complement(43033..44406)
                     /gene="glmU"
                     /locus_tag="U3995_000253"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1795605.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44909..45727)
                     /gene="purR"
                     /locus_tag="U3995_000254"
     CDS             complement(44909..45727)
                     /gene="purR"
                     /locus_tag="U3995_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1795606.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45894..46718)
                     /locus_tag="U3995_000255"
     CDS             complement(45894..46718)
                     /locus_tag="U3995_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1795607.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46691..47371)
                     /locus_tag="U3995_000256"
     CDS             complement(46691..47371)
                     /locus_tag="U3995_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1795608.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47394..48332)
                     /locus_tag="U3995_000257"
     CDS             complement(47394..48332)
                     /locus_tag="U3995_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1795609.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48577..49440)
                     /locus_tag="U3995_000258"
     CDS             complement(48577..49440)
                     /locus_tag="U3995_000258"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1795610.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49582..52155)
                     /gene="mprF"
                     /locus_tag="U3995_000259"
     CDS             complement(49582..52155)
                     /gene="mprF"
                     /locus_tag="U3995_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1795611.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52296..53102
                     /gene="proC"
                     /locus_tag="U3995_000260"
     CDS             52296..53102
                     /gene="proC"
                     /locus_tag="U3995_000260"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1795612.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53181..54737)
                     /gene="rny"
                     /locus_tag="U3995_000261"
     CDS             complement(53181..54737)
                     /gene="rny"
                     /locus_tag="U3995_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1795613.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55148..56197)
                     /gene="recA"
                     /locus_tag="U3995_000262"
     CDS             complement(55148..56197)
                     /gene="recA"
                     /locus_tag="U3995_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1795614.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56306..57547)
                     /locus_tag="U3995_000263"
     CDS             complement(56306..57547)
                     /locus_tag="U3995_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1795615.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57787..58365)
                     /gene="pgsA"
                     /locus_tag="U3995_000264"
     CDS             complement(57787..58365)
                     /gene="pgsA"
                     /locus_tag="U3995_000264"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1795616.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58574..59497)
                     /locus_tag="U3995_000265"
     CDS             complement(58574..59497)
                     /locus_tag="U3995_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1795617.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT        16535 a        13068 c        10751 g        19628 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    21601 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    21661 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    21721 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    21781 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    21841 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    21901 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    21961 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    22021 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttgttga
    22081 agattcaggc actgttgttg attcttctgt tgctgaagat tcaggcactg ttgttgattc
    22141 ttctgttgct gaagattcag gcactgttgt tgattcttct gttgctgaag attcaggcgc
    22201 tgttgttgat tcttctgttg ctgaagattc aggcgctgtt gattcttctg ttgaagattc
    22261 aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct gatgatgatt cttcagtcat
    22321 tgaagattgt gtagctgatg attcttctgt tgctgagctt tgtgcagtag atgaagcttg
    22381 agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct ttttcagctt tttcttgtgc
    22441 agcttgttga cgagcttgtt ctgctagacg ttgttgttca cgaatacgag cttgctctgc
    22501 ttcagcttct gctttttgac ggttcaagtc agctttcttg tcttcagcag ttgcttgttc
    22561 agcagctaat gaagttttca gtacgttcaa atcagcttgt ttagataaaa gatcgccttt
    22621 ttgagattcc aaagcagctt ggttttcagc taattctttt agtttagcat cgttttcagc
    22681 ttttttgtct tctacagctt ttttatcttg tttttgttgt tccatcaagt cgttgtttgc
    22741 ttttaccatc gttgtcattg cttgaacgcg tccaatagca tctgctaatg aatcagcatt
    22801 caatacagca tcgatgtagt ttgagcttgt gttgcttact tgtgcttcgc gtgcttgttt
    22861 ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa tctgcaatgt cttttactaa
    22921 ttgtgcagat tcttgacgca atgtatcttg ttttgctaat aggtcttgtg cttgtgtgtt
    22981 gatttcagaa acttggcttt ctaaggcatc gatttgtgat tgtgcatctg cttgttgatt
    23041 tttcaagtct gcaatctttt gatcttgctg ttgtatctga gaatcaaaat cgtccgccgc
    23101 ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa cataccatta ctgctgatat
    23161 taaactcttt ttcactcttc attcctccga ctggcttaat ttaataaata tctaaatata
    23221 tttaagtctt tcttaacgat ataaaaattg taccatagca gtgtgtcaac gatataatag
    23281 ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga caaaactgtt cgtttcagaa
    23341 aatgatcatg aaaaagccgc gttaagtcta gcatttctta agataacaac gattcttttt
    23401 cgctttcgac ctaaaaataa aaaaagaaat tattcatcac tgaataattt cttaaaggga
    23461 aaaatgaata atacaaatat aatcatgttc aacagcagtg taggtcctaa aaaccttgta
    23521 ataaaatacg tgttatttac tactgctaat ttaaaaatca actggaccac catcgtaaac
    23581 aattcataac ccgtgacaaa gatgatcata ctgaaaaatt cagtaaagat gttgacatgg
    23641 ataacgtctt tcattacata catcaaccat acaattaaag ggagagctac tgcatagata
    23701 ccgataacgc caatatagta agcgtcatag atagccccga gaacaatcgt ggtaatcagt
    23761 aaataacgtt tttcaaacgc catactgcaa cataataatg ccaatatcag aaagtgggca
    23821 tttgacatat aggtgccttt cgaccactcc cctagcattc ttgttaaatg accatctatc
    23881 aacatcaaaa agaacaaaac gattggcaga tagtacttca ttgtttcttt cttcaacatg
    23941 gactaatcct ccgctagccg ttggacaatc gtcacaactg acacgtcata catttctgca
    24001 taaggtttca cataaacttc ccgatccagc ccataactat ctggtttcgt tttaataacc
    24061 gtaccgatcg gcaagttagc tggagaattc cctccaagac cggatgtctg gacaacatcc
    24121 ccttctttga tatccgtatc accagttaat tgggtcacca ctaaggcatg gagcttttca
    24181 tcatagtttt taagcaaacc aaacgcttcg ccattagccg aagatacccg tactggaaaa
    24241 tgattggagc tttcattaga ggatgacagt aattcgattt tagacgaagc cgtattgacc
    24301 tcgatcactc ggccaatcaa tcctttttgc gacatgactg ccatatttgc ttcgataccg
    24361 tctttagaac ccttgtctac gatcagcatg tcctgccaac tgtcaggcga acgagtaatg
    24421 acatttgccg ttactttttc gtaactagtc agcgtctgat tcaactcaag ttcttttttc
    24481 aacgcttcga tttctctctc gtaattcttg ttttgctgtg aaacgtcatc atagctatcg
    24541 attttttctt ttagccgctc attttcggca taagttgaaa aaagattatg gacagaatct
    24601 actccatttc gtacccaccg tactggggca gagattgttt tatcaaccat tgcaacactg
    24661 tcattaacga tggattggaa gaaattgggc tttccttcat tcgctcgctg cgcagcggtc
    24721 acacttagta tcgtcaccac gatgataaca ataaccaagg taataataat atttttatta
    24781 ggattgaact tcttcacaaa gacacctcga attaattaga ctcttgtgtt caattttatc
    24841 actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa gctttttttt agcaaataat
    24901 agattctgtc atttttagcc tgaaagacaa atttttttat ttgtttatgg atcaatcttt
    24961 gctttgaata actgcaatca taccatctga aaaagaaaat tctgcggtat ctgaaagaaa
    25021 ttcgttactt gcataagatg tcggatattc tacttcttta ttttccaata catacttgct
    25081 gccttttaat gtaagattcg agacgggagt caagcagcaa taagctaaat acttcatatc
    25141 tggtatctgt cttactatat gctctccagg caaataataa gacaaataat tttgacaatc
    25201 tctgataata agctgatggg caaagggttt gaagcgaggt tccataccta accagagatt
    25261 ggctaaaaga tgatccaatc ttccgcccgt agctccaatc aaagtgactt ccgcttgcgg
    25321 gtaacgttca aatgttttta agatacctaa ctgtgtatct gtatcgtctt tttctgcagg
    25381 ggaacgggta tactctttgg cttttgcaaa tacttccttc tgctcttcgt tcgacaatga
    25441 atcaaaatca cccactgcca gatctactgg gtagcctgct tgaagcaggt acagcgcacc
    25501 acggtctatc ccgacatagg catcgtattt ggacagcaga gactgcggcc agttttgcgg
    25561 attacctcca gcagctaata aaattttcat ttagtctaat gcctctttta atcgttcgat
    25621 ttgtccgact ggattttcgg ctccatagat atatgatcct gcaacgaaga catccgcccc
    25681 tgcatcttta cagatttttg ctgtttctgg aacgattccg ccatctactt cgattgcata
    25741 agtccaattg ttctctctac gtagatcagc gagttccgta attttttcta ctgtttcttc
    25801 aataaagctt tgtccgccga acccaggatt gacagtcatg actagtactt gatcagccaa
    25861 aggcaaaaca tgtttgatca tggagactgg tgttcctgga ttgataacta ctccagcttt
    25921 aactcctaag ctttttgtca tttgaagtgc ccgatgaata tgaggagttg cttcgacgtg
    25981 gacagtaatg atatctgctc cagcttttgc aaatgctgga atgtagtttt ctggatcaac
    26041 gatcatcaga tggacgtcta acggtaattt agtaacagga cggattgcct gaacaatatt
    26101 tggccctaat gtgatatttg ggacaaatcg gccatccatt acatctacgt ggatgtaatc
    26161 tgcgcctcct ttttccacta actcaatgtc tctttgtaaa ttggcaaaat ctgcactcaa
    26221 aatggatggt gctatcttca ttcttgtttc ctcctatttt cgtttacctt ctttttttgc
    26281 atacattggt tttctttttt cgatttcttg taagaattgc aaataattgt cgtaacgtgt
    26341 ctgtgcgata ccacctgttt ctactctatg ttttacttcg cagccaggtt ctttagcatg
    26401 catgcactct ctaaatttgc aatggacaga agcttctaca aattcaggaa attgtctcgg
    26461 cagttctcct gcttcaattg tcaaaaagtc aatcgagcta aatcctggtg tatctgctac
    26521 taacccgcca tagataggaa tcagttccac atgacgtgtt gtatgccgtc ctcttccaag
    26581 tgcatcagaa atctctcctg ttgctaaatt cagttccggt gatatttgat tcagtagcgt
    26641 cgattttcct gctccagact gacccataaa gaccgatagc cgttctggga agtaacggat
    26701 cagttctgtc gtatcacccg tatgattggg tacaatcacc ggatagccta ttttagtata
    26761 ggtttgcttg atctcttcga ttttttctgg gtcgtctgac aagtcggtct ttgtcaaaaa
    26821 aataatcggt tcgatcgact cgtattccaa ggtaactaga aagcggtcaa gtagattata
    26881 agaaaatgca ggctctacga tacttgtgac aatgattcca agatcaacat ttgtgactgg
    26941 tgggcgaact aattgatttt ttctcggcaa gatttccaga agatagccgt ctgtttgatt
    27001 ttcactctca aagatcacct gatctccgac aagcggcgtg attttccgat tccggaaatt
    27061 tcctcttcct cttgtttgat aggtctctcc gtttgcgtat acatagtaaa aaccgctgat
    27121 tgcttttctg atttgtcctt tgataagtgc cacctccgtt tttgtttatt ttaccatacg
    27181 aagaggaaag taggtgttat tccatgcaaa cttctcaaag tatatggctt gaaaattgat
    27241 caaaagagat agaaacacaa aaaagagaat gcaacaaatc aatgtcgcat tcccttctga
    27301 tatattttat tcagcactta ttttgaactt tctgaggtgc tagctgatga acttgtggag
    27361 ctggaagatt cttcttttga agagttgctt gtgctactgg agctacttga gctgctagaa
    27421 ctgtctgaag agtctttttc agaactagaa gacgctggat ctggtccttt agagtaaatt
    27481 acgttcacaa ccattccttt ggtaattgtc gttcctgccc ctggtactgt acgaatcact
    27541 ttatctttat ccaccgtgtt tgaatattcg gaagtctcat gcccgatata ttctgcaccg
    27601 acactagcta aataactttg tgcttcgctt tttgtatagc cggagatatc attcaattta
    27661 ggctctgttc cttggcttac gacaaaattg atgtaagttt cttctgctac aacttcaccc
    27721 gaggctggat cctgactgat gatcgaacct gatggtacag tgtcgctata gtcataagta
    27781 gcactaatat tattgatatt gaatccttgt gtaacaagcc aattacgaac agtttcgtag
    27841 ttttctccta catgactact caaagtcact gtttctggtc ctttgctgac tgtgaaagta
    27901 atcgtgtctg ttttaggatc gacttctgta tcttctgatg gagattgcga aatgatcatt
    27961 ccgctttcta cctctgaact gaattcctct tctttttgaa tcaagtcagc agaaaaccct
    28021 aattctttca aacttgcttc ggcatcttca taacttttac cactgtaatc atctaatttg
    28081 atttttttcg tgccgctact aatatacaga gtgacttcgc gtttttgctt gactgtcgtt
    28141 cccgctgccg ggtctgtctt cactacatta ccttcttcga ttttgtcatc tgcaatttct
    28201 tctgtttttg ttgctacttc taatttcgct tttgcgagtg tttctctagc agaagcttca
    28261 gaaagatcag aaacatcagg aacaacaact tctcctcgcc cgccagaagc aaagaataca
    28321 gctgttccga ttcctaaaac agcaagaata gctaatagga tcagccacca ttttttctgt
    28381 cctttctttt taggatcagg cagaacttct tcctcctctg gtaaggtttc gttttctggc
    28441 ttgtcttttg gcagaggcat agatttgaat gaatcaggca ttggtgcatc ttccgtaagg
    28501 ggtgtgataa cttttgtttc attatccatc acatgaggct gccatttttc ttcgtttgca
    28561 cgagctggtg ataatactgt agaaagatca tcagacattt cctcggctga cttataacgg
    28621 tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt ccagtgcttg tggcacattt
    28681 ggatcaagtg cttttataga tggaagatca tcttgaaaat gttttaaagc aattgttaca
    28741 gcagattcac catcaaacgg tacgctaccc gtcaacatct cataaaggat gatccctaaa
    28801 gcataaatat ctgactgctt cgttgccata cttcctctgg cctgctcagg ggacagataa
    28861 tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg tttcagataa agcgatagca
    28921 atcccgaaat ccgtgatttt tacaacacct tcattatcga tcagtacgtt ttggggtttc
    28981 aaatctcggt ggatgatttg atggttatga gctaaagaaa tagcagaaag gatctgctgc
    29041 ataatattta ctgctgtttc ataaggaaca ggataatgtg tttggatata tcgtttcaag
    29101 tccattcctt tgacgtattc catcaccaga tactgcatgt tgtcttcttc accaacatcg
    29161 taaacactaa caatgtttgg atgaacgagt tctgttgctg ctaaagcttc tctctgaaat
    29221 cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta agactttaac tgctacatca
    29281 cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca taccaccgct accgatattt
    29341 ccagtgattt gataacggcc atttaatttt ttgcccaact cgatcataca ctgtcctcct
    29401 tatattcgat aagaagaacg gtgatattat ccgctccgcc tgcttcattg gcacgatcaa
    29461 tcagctcagt tactttatga gacaatgggc cttcttgatt gatgatcgtt cctatttctt
    29521 cttcagacag catgttcgtc aagccatctg aacataacag cagtcgatct tttagttgcc
    29581 aaagatgagt agaaacatct acttcgactg ttccaggcat gccgacagaa cgtgttaaaa
    29641 tatttttcct tggatggttc actgccattt cttcgctgat ttctcctgat ttgactagtt
    29701 cattgactaa agaatgatcc tccgtcaatt gcatgatttt atcttcacga atcaaataag
    29761 ctctgctatc cccaacattg gcaatcgtga accagtcatc tgacagagcg gccgctacga
    29821 ttgttgttcc cataccgtta tattccggct gttcttgtcc acgctgataa atcagcatat
    29881 tttcttcttg aatcgattga atgaaccatt gggcgatttt ttcatcgtca acagcttctt
    29941 gtttttccca agcttcgcct agattcgtta ccgccatctg actggcaatg tctccggcac
    30001 gatgaccacc catcccgtct gctaatacag caagcttgag acctgaacgg ctggtaaata
    30061 cactggcata gtcttgattt gtgttacgtc gtcttcctac atctgattga tattcaatct
    30121 gcattttttc acctcattac tttttgcgca aacaacaaat gaaaaatcca tctgtgtaaa
    30181 actgatgcgg atacaaagtc aacattttct cttcgattga tgattgtagc ataggattgg
    30241 tcaaaacatc aatcctctca aaatcagggt gtctttccaa gaaagcagaa acaacgtctt
    30301 ggttttcttc ttttaagatc gtacaagtgc tataagtcag aataccggaa gactttaaag
    30361 ttggtgcgca gctttctaaa atttccaatt ggatttttgg taaattcgct aattcatccg
    30421 gctgtttttt ataacgaata tctggttttc gccgaagcag cccaagacct gaacaaggcg
    30481 cgtctactaa aatccgatca aactgctctg cttcgaactg tttactgact tctctagcat
    30541 ccatcttttg agcgtagaca gcagaagaga cacctagacg tgctgcatta tcttcaatga
    30601 gtttgacttt gtgttcgtgt acatctaaag cgaccacttc tccaccgcat gacggatcta
    30661 aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc acaggcatct aagacatgat
    30721 gttcaggctc gatttgcata gacggcgcta caagcatcga actttcatct tggatcgtca
    30781 tgacgccatc gtggaacaat gagctagaag ctaaatgccc ttttttcgct actataccgt
    30841 atggactgac ttggcttttt tcagctgtga tctcttcttc ttgcaaaaca gtgatggcct
    30901 gatctctgga aagtcggcgt gtatctactc gggcactagc atggctaggc tgatatagcg
    30961 ataaaccgag cttttcagtt tcttcgtatc cgatttgatc caccagtcgt ttcgtcatcc
    31021 atagaggcag gctgattttg acagaaagtc tttcgatagg atcagcaatg ccttgcagat
    31081 ctggtgctcc ttgacgctga taattcctca agacaccatt tacaaatttc cctgtcccag
    31141 gatttccttt tacttttgcg atttcgaccg cctcgtttaa aatagcatgt gcagggactt
    31201 tatctaaaaa ttctaattgg tatacagaaa gataaagaag catcttgacc cagtcatcca
    31261 ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc caatagtcgc tgatggctaa
    31321 tcgtaccata gatcaattct gtaaataaac ccacatcttt ttgatttagc tcgctttgtt
    31381 tgatcatctc attcaacaaa agattcgagt aggcgccacc cttgttcact cgctctaatg
    31441 tcagtaatgc catatatcgt actgattttt tcattttttt agggattttt ttcattattt
    31501 cacctgctga ccaatctgaa cttgctgacc gcttccgtta aggaaatcat gtacggcctg
    31561 ttttccttta ccagctggct gtaattcatt gatggctaag acggtctgtt taccgcaagc
    31621 aatccataga ttttttttgt ctttccggat gatcgtacca ggtgctttgt ctgtagtttc
    31681 tgccaatact tcaaccgata ataatttcca gcgtatgccc tcataggtcg tgaatgcaat
    31741 tggccaagga cgcattccgc gtacttggtt gtcaatttct tctgcagttt tatgccaatc
    31801 gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt gcttctgact cgtcttgagg
    31861 tactggtttt tgtccatcca aaatattggg caatgtttct agcaatagct ttcttcctaa
    31921 aaggctaagc ttttcaaaca tcgtccctac atcatcttgt ttagtgattg gtatgctttc
    31981 ttgcgcatag attccgcctg catccatttt tttgatcatt tccatgatcg ttacaccagt
    32041 ttctttctcc ccattcatga tggcataatg aacaggcgca cctccccgat atttaggaag
    32101 taaagaagca tggacattga ttgcccctaa ttttggtact tgcaataact tttcaggcaa
    32161 gaattggcca aatgccgcag tgacgatcac atcaggcacc aattcaatga tttcttccat
    32221 ttctggcgaa ccggagattt tctctggctg cagcacacgg ataccgtgct tcagagccgc
    32281 tttttttacc ggtgtcggcg taattactcg tttccgccca actggacggt cgggctgtgt
    32341 taccactgcc tggatctcgt aacctgattc tatcagccct tcaagaatcg gcacagaaaa
    32401 agcaggtgtg cccataaata ctagcttagt catcaagatg ttcctccata tattcttcaa
    32461 gttcttcttc cgggatctgc tgtatcattt tctcaatgaa caagataccg tctaagtgat
    32521 cgatttcatg ctggaaggca cgggctaaat agccaaaagc cgtcacttct atctcttctc
    32581 cgtctcgatc ataataacgc acggtcactt catctgctcg cttgaccgtt ccatagacat
    32641 gcggaatact gagacaacct tctacgtcaa ggctttcacc ttttgcttcg atgatttctg
    32701 ggttaatcag ttcaaatttc tcgccttcat ctacttcgat cacagcaatt ctcttgtttt
    32761 gtcctacttg aggagcagcg atccctattc cgtcattcgc aatcattgtc tcatataaat
    32821 tgtctaataa agcgattgtc tcgtccgtga ttacatcgat tggctgagca gttcttttta
    32881 atttatcatt agggtggatc aatataggat agcgcataat ttggcccttt cttaaataaa
    32941 gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt cgctgttctt tctggcttcc
    33001 attcaaaatt tcatccaaaa catgaggaag attcggttca tgtttatatt tgatgattag
    33061 ttgataataa tatcggtttt tcactctcaa aatcatgctg ggcgttggcc ctaatagcaa
    33121 gctttgaggg cttaacactt cttttagctg attggcaatc tgaaagattt tctttgccgc
    33181 tacttgttct tcaggatggc tggcggttat tttcacagta aagtaataag gaggatagcc
    33241 tccacgatgc cgtacatgca tttcctgctg ataaaactgc tcataatttt gttttttagc
    33301 caattctatg gcgtaatgtt gaggattgaa tgtttggatc acgacttcgc ctgctttttc
    33361 cgctcgccct gctcgtcctg aaacttgggt aagcaattgg aatgtatgct cactagaacg
    33421 aaaatccggt aaattcaggg aggtatcagc attcaacacc ccaactaatg tcacttctgg
    33481 aaagtccaaa cctttagcaa tcatttgtgt ccctaataat atatctgctt cttttgcacc
    33541 gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga gtggtatcta catccatacg
    33601 caaaatccta gcttgcggat aaagttctcg gagttcctct tctacttttt gagtacctgt
    33661 tccatagtag cggattttat tgccgccaca attaggacag cgattgggaa taggttcttc
    33721 atgtccgcaa taatggcagc gcattgaacg tgtatccata tgcagtgtaa gagagatatc
    33781 gcaattagga catggaagaa caaatccaca gtccctacac atcacaaaag acgaatatcc
    33841 tcgacgattg agcagtagga cagtttgttc ttttttgtct aatcgatttt gtattttttc
    33901 ctgcaaatta gcagaaaaag tagaggtccg atgattctga aactcttcac gcatatctac
    33961 gatctcaata gctggaagct gtgcagctgc tttggctcgg tggtttaact gcaataactg
    34021 ataaacgcct ttttgagctc gtgctcttga ttctaaagag ggcgtcgcgc ttcctagaac
    34081 tattgggcaa tgatgatatt tactgcgcca aattgctaga tctctcgcat gataacgcgg
    34141 tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg ataatgatca gcccgatatt
    34201 tttcagcggg gcaaaaatag cagatcgtgc gcctactact acttgcgctt cttcccgctc
    34261 aatcttgcgc cattcatcat atttttcacc ttgggaaagt ccgctatgta agaccgcaac
    34321 tgattctccg aagcgtcctt tgaatcgctc gaccatttgc ggagtcaatg cgatttcagg
    34381 cactaacata atagccgttt tattttctga caaaacatca gcgattgcct gaagatatac
    34441 ttctgttttc ccgctgccag tgattccctc cagcaaaaag acctgatctt tttgctgctg
    34501 acctgctgtg atgatttgtt cgacagcatt tttttgttct gcattcagtt ccaatgccgt
    34561 cgtttggtca aatactcggt ctttataagg gtcgcgataa cgttctgatt ctataaactc
    34621 cagccagccc ttttcttttc cttgattcaa gacagcagta ctgattcctt tatttttgaa
    34681 ataagcaatt ggttcttctt ggctcagttc agataaacac aaaagaagtt ctttttgttt
    34741 cttggcattt tgccgtaatt ttgcccattc ttcttcgatt tgcttttcag ttaacaaaga
    34801 acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg acttcatatt tgatatctac
    34861 taattgctct tggcgccagc gaatcaattg agaaaggctg ccttcttctt ctgcttcctt
    34921 ccaggaaatc acttctcgct gatcaaacaa tacctgaact tctggtgtgt ctgccaaagg
    34981 atagatcaat ttatcatatt ctgctttcat cacactcgga agcatcgttt gtagacaagt
    35041 gattttgaat gcgtaagtaa tttttttcat ataatcagct aatgaaagta attcttcatt
    35101 gaccaccgga gcaaggtcta gcaaacgaat aactgccttc aaagatggat tagctgattc
    35161 ttctgatttt tggatagcca taacaaatcc ttggacatga cgattgccat taccaaaagg
    35221 gacttctact cgcatcccga cttgcaaagc tgtttccatt tcagagggaa ccaaataagt
    35281 gaatggctga tcggtctgca tagtaggtac gtccacaatg acttcagctg ttaatgtcat
    35341 ggttcgtttc ctcctttttt tgtcttttct tatcaagcga caaaaagttc cttgcctatt
    35401 ttactagaga tcgatggatt tgtcttatct tctgccaaat cctaacaaaa aattaacagc
    35461 agataagaaa aaaaagaact gtgtcaaaaa gttcagacac agccttttct accagacaat
    35521 aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt ttctttttag atgttcgctc
    35581 tatttttctt tattacattt tttgctcttc acggatacgt gattccaatt cgtgttgttc
    35641 acgctctttt tgtgcttgac gttcctcttc ttccatcttc aaacgttcac gttttaattc
    35701 aggatgtgga tcattgacta catttcctgc atcgatttct tctaaagctt gaccgacgct
    35761 ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt gcatccaatt catgcgcgcg
    35821 tttacttgct aaaatcacta gagaatattt tgaatttaca cgatctaata gtgagtcaat
    35881 tgaaggtttt aacatcataa gactacatct cctttaacat tttaatatat ttaccgatta
    35941 cgcgatctac tcgaaaatgt tcactggcaa taatgttttt gattcgttca actgctagcg
    36001 gcacttcatc atttacaaca gcatagtcgt aaagagccat catttcgatt tcttcttttg
    36061 caactttcat tcgtttttcg attacttcat ctgcatctgt tcctcggcct acgatccgtg
    36121 attttaattc cgctaaatct ggcggagtca aaaagatgaa tacaccatcg ggcacttttt
    36181 ctttgacttg ttttgctcct tggacttcaa tctccaagaa aacatctttt ccttcatcca
    36241 atgtcttgtt gacatatgac aaaggagttc cataataatt cccgacatat tctgcgtatt
    36301 ccagcatctc tccagcttcg atcattgatt caaactcttc tttgcttcgg aagaaataat
    36361 caacgccttc gacctcccct tcacgcattt tacgggtagt cattgagatc gaatactgaa
    36421 aatcattatc ctcactctca aaaattgctt ttcgtactgt gccttttcct acaccggatg
    36481 gaccagacaa cacgattaat aatccacgct ctgacatgac gacgtccttt caactagtct
    36541 ttttgtactt atcgtttatt ttgcactttt tttctgcaac tttcaaggtt atctttaatt
    36601 atagctagaa attggtaaaa aagctaccct gtaccaagag gtcgtaaaaa agctgtcctg
    36661 catcaagtaa taagaacagc caaacataaa cagcttctca tgtttttcgt ttggttgggg
    36721 ttattatcgc agatgcaagc ttttgaacgc cgtgtataag aggtcgtgaa gaagccgatt
    36781 agtttcgagt atgaagaaaa attttgtgaa aatagtttct cacatttttg cataaatttg
    36841 gcttaatacc gagaaaatgg tttttgaaca tcacttattc aaaataaaaa aagattgtga
    36901 cacgaatttt gtcacaacct ttaaatgtat tcctagagca aagaagctaa gcgaagaaat
    36961 ccaattctgt ttatttattg tatttctttg ttcagcatcc ttgctaaaag cccatattgg
    37021 acaaagtatt cactacaccc atgatctcat cttttcgaga acgtgctttt tctgtcggat
    37081 cttctacctc ttctgtgtca attcgaacaa taaatttccg attcatcgtc acgtaagcac
    37141 cgtcgttgat caaaataaac agttcgttct cattatagtc tgcacaggca tctttccata
    37201 cgtcaaagcc gtttttttca gaagggatat cccgtttaac cacagttttt ccacttaatt
    37261 cgtggaacgt tacgtcataa tatttcatta tctctacctc tttcgtcatt caatattttg
    37321 tacttgttct ctgatttttt ctaagatcgt cttagactgg acgacttgat ttttaatttc
    37381 gattgcattt gatttggaac cgatcgtgtt gatttcccga ttcatttctt ggatcaggaa
    37441 atctaattcc cgacctacag gacattgtgc ttcgataagt tcatctagtt tttgtacatg
    37501 gatcgccaga cgatccagtt cctcgtgaat atctccgcgt tccaacaaga tggctagctc
    37561 cgtcaacaac cgttgctcat cgactttcgc tccaagaaat tcctccagct ttttttgata
    37621 ccttatttga aattcctttt catagatttc tacaaaacta tcgagttttt ttagtacttc
    37681 tttgaattct tcagtattct ttttcaacac taaagctaaa gcttgacctt ctttttctcg
    37741 actcgcctca atagaatcaa gtgcgctttt caatgtatct agagctaact gtgccaattc
    37801 ttccatgctc tcttccggtt tttcttctac cgttacgaag ttttcgtcca gcacgaagcg
    37861 ttccgccatc cgactaattg acacttgatt ttgactaccg tatcgttgtt gaattccgtc
    37921 tgtcaattga gtgataagtg aatcaatcaa ttcccagttg atgacgatct gtttttcgtt
    37981 tttgccaata taagctagat tcacaaatac ttcgattcga ccgcggctta actgctgttt
    38041 aataagctga cgaaaatcgt tttcaacaaa gttcaggatt ttaggacttc gtatttgaac
    38101 atctagaaaa cgctggttga cactttttat ttcaatatct aactgatacg cttttgtttc
    38161 gcggatggct ttgccaaaac cagtcatact tttcattcga cagggtcctt cctcattaca
    38221 tattttctat tgctttttgc agttgttcaa actcttcgtt cgtcaatgtc aggcctttcc
    38281 ccattttttc atggtctggc gcccaatctc gaagatcgaa ttttggcggg cgaccattcc
    38341 agctgactag gttcagttct ttacgccacc ccttgttgtt ttctgacaat atagcgattt
    38401 cttctacgat ttcatatgaa aattcttgtg ccatggttgt tcctccttga gataatataa
    38461 atataggatc agtaatgtgt caactggttg cttaagcaat tcatgctgca aacgatcctt
    38521 gcgttttcct tgtagcagat aatcaaaata aacagttttc gtgcgctcat tttgtatttt
    38581 ttgctggtat tttcgatacc ttcgccattc ttcagtactt aattgaccaa tttgtgccaa
    38641 taaatcatcc ggcatacttt ttatatcttg ttggcagatc atttgaatca tcatgtctac
    38701 ttgatagatt tcattgggca gtaaaaaatg tttttttgct aaaagttcta gcatcgccaa
    38761 tccgcatgtc tctggtacga ccctgttctt atataaatta cgcagttttt gccaaaattt
    38821 tatgcttttg aaaaaagata attctaactg aatttctttt gttaagtaag acaaaaatcc
    38881 tcgccagtat gttcttgcag gtttaacaga atgaaatgta taccagttcc catagtaata
    38941 gcctaatcgt tttttcattt ccagatagcc taattgtaaa tttaattggt tcctttggct
    39001 atcaaaaagc agaaagctgc ctagtgtcca caatgtttga catttccaat ggataaaaat
    39061 gtctggcata cgaatttttt tcgctgggcc tggcccttgc acatctacga caaatgcctc
    39121 tgttgctcct ttgttaatcg caatatcaat aggtattttg ttgcgatacc caccgtctgc
    39181 atatttattt tttccgatct ttcgataagc catagcggga taaaatgaag cagaggcgag
    39241 tatccaatct ggtatttctt ctgtttttgt ctgttggata tcagtcacta cttccgctag
    39301 ttctggcagt ttagtagaca ccgtatataa acgtattttg ggattatgtt tgagtaagga
    39361 caaatccaaa ttgtctttga ccatcttttc caatggagca gaagaaaccc cgccttcaat
    39421 tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag cgttcacgaa ggtcttcaac
    39481 tgcagccatt tctggtaacg caagcacttg tcttgtcgac agctttttcc ataaattcac
    39541 agcttttttc atatctcctt gtaaaatcaa tgcgccgttc aacgcaccta ccgaggtacc
    39601 tgtgataatc tcaaagttga tatcatgttc cttcagcgcc tgccacacac caatctgata
    39661 agcaccgtgc gctcctcctc cacctaaaac aagtgctgta tgatagacca gctttttatt
    39721 taattgtgtg gttccttgat agccgttttt atgaaaaata cgcaaccatt cttctgatag
    39781 aggagattga aataaaaaac aacagttttc tttaataaaa aaacgagcgc aagcttccat
    39841 ataccgaaaa aattttttcc attcaaaagt ttcaccttca atcaacagat tattaaaaat
    39901 aacatctcct ctacttttag caatagtaaa atagacggta tattttttcg atactgcttt
    39961 aaaagcacat tgcttttttg cagaacgcca aaaagcgtct ctactttctt gatgactttg
    40021 aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa tacagcgaca ttttctgtga
    40081 aaagtaaact ttttttatct ccatcgcgct ctcctttttc ttcaagtata ccagtacgtc
    40141 ataaaaaggt catgagtctc gtattcgact ttgtagattc cataagaaaa caattcataa
    40201 aacctccttt aaataaaaaa atacaaaaat cacaaaaatt aattgaaaat gagagtgttt
    40261 tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa agaaacaagc ttttttaatg
    40321 caaatgttgt aaaataaaag agattaagag gaaataagag gaggaacatg atgactgagt
    40381 cagtttatgt acatattgat accacaagta atgctgtttt aacaaaaggg ctgactgcta
    40441 ctgactttgc aaacagtatc gtgcattttc ctcaaaactt gctcttgctt gatccatctg
    40501 cttctgctgg agaatatgaa tcccatacag ggctgaaagt aatccgtggt acagaaaata
    40561 ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt cgatgaattg aagtggatcg
    40621 actttactga tttaacgatg ctgaaagaat tgacaccttt agaaatttca gaattattat
    40681 attttggtca tatgaaaaca catcttcatt cgccgttttt ttataaacta cagaataatt
    40741 ttgtatactt cgatcttaat gatcagctga atcggattta ttatcgctat ttagatgaat
    40801 tttataggat ttttgctagt aaattaagtt cgatcttgtc agataagatc aatgtaaaaa
    40861 aatctttttt caaaaaagaa acacctgttg aaaaaatcag tgtggaatta ttgaaagaaa
    40921 tgcgtaattt gatgcaggaa gggatcattt tttcttttga tcaagttggt ttgatcgatg
    40981 gtaaatatcg tatcccaatc tatgtgattg aagattatat ttggaaatta aaaggcaccc
    41041 ggtatcgaga agaggaaatc atcgctactt taatctactc tgtcaccgat cgacactggc
    41101 aattggtaga agatgccgat ctttcttatc tgccattgaa gtagatctat ttcttatcaa
    41161 aaaataaaaa ggaaacaaga aactaggaca tgattctgtc tcaagtttct tgcttctttt
    41221 ttatttacag ctcctgtcgt ttatctggca caaaaatcga aggagtcgtt tgttggatcc
    41281 ctaataaaac gatgatcgtt acaatagctg tactcaaccc gctggcacca ttcattacga
    41341 aagaaaagag ccaaggattc atcccccata gtgcatacgc tccccagaaa accactcctg
    41401 caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa tgcaccaaac acgatgcttt
    41461 ttctaatttg tttcgtatta tcgtctacat agcctttttg gacatttgaa gccatgagcc
    41521 ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat caacacttga acgacactta
    41581 aataataaac ttgtcccaac ggaaaatgta ggatgcccca aatgaaacct gccagcatcc
    41641 ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg aatctgccct aatgaaatag
    41701 aaaaactgct gccgatctgg atcggaatca acgacaacac catcgctaac gctgcaacaa
    41761 tcgttccttc tacccaaact ctattttttc ccataaaaaa acttcctcct ttgaataaaa
    41821 aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac agctgtttat tcacaattcc
    41881 tacgctcgca taaccgatac aggtacgaag ggtttagaaa tagattcaat ctcagcccaa
    41941 ttggactccc ctttgtgagt ttatttatta tttatttttt gtttcaaaca atggactaac
    42001 tggtttgttt tcatggatac gtttgattgc atctcccatt agtccgccaa cacttacttc
    42061 atcgattttg tcgattttac gatcttctgg aagatagatc gagtctgtaa caactagacg
    42121 ctcgatagct gaatcttcta ttctttgtaa agcagggccg gataatactg gatgtgtaca
    42181 tgaagcatac acactagttg caccagcttc tttcaaagca ttggcagcca atgtgatcgt
    42241 acctgctgta tcgatcatat catcgatcag tacacaagtt ttgcctttga cttgtccaat
    42301 gatattcatt acttctgcaa catttgcttt tggacggcgt ttgtctatga tcgcaatcgg
    42361 agatttcaaa aattccgcta atttacgtgc ccgtgtgaca ccaccatggt ctggtgaaac
    42421 aacgacaaca tcatcgcctt tgattccttt ttctaagaaa taattggcaa taagtggcgc
    42481 acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg atttggactg catgaaggtc
    42541 caaagtcagc attcttgttg caccagcttt ttgaatcatg tttgctacta atttcgctgt
    42601 gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca tagccataat aaggcatgac
    42661 tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca atcatgatca gcaactccat
    42721 caagttatca ttgactgggc tgctcgttga ttgaataaca tagacatgcg atccacgaat
    42781 actttcttca atgtttactt gaatttctcc atcactaaat tgagtaacag atgattttcc
    42841 taactcgacg ccgactgctg ctgcaatttt ctctgctaat gggcgattgg aatttaacgc
    42901 aaaaattttt aatcttgggt caaagtaatg ttttgacatg gggacctcca ctttcttttt
    42961 cacaactcta gaattctacc taataaatag tagtcatatt ttttaaataa atcaagtgat
    43021 tttgcaaaag tcttagttta gataagggag tttttttgca tatccttctt tattgacttg
    43081 tcttgctctc gcaattgcca gcgcatattc tggaatattg tctgtaattg ttgacccggc
    43141 agcaatcgaa ctgtttttag ctacctcgac aggtccgatg atattcgtgc tagaaccaat
    43201 aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt ccatcatagt tgacgaagac
    43261 aacaccacat ccgacattga tttcttctcc cagcgtagca tcaccaacat aagtcaagtg
    43321 accgacttta gttcctttgc cgatttgagc atttttcact tccacaaagt ttccgatatg
    43381 gacgttttcg ccaatttcag cttttggtcg taaatgtgca taaggtccca catctgcatg
    43441 tttcttgaca tggctgcttt caatgactga gttttcaatg acgacgtgat cctcaatcct
    43501 actgtctacg atttttgaat gtgacccgat gacacaatca gaaccaatga ctgtatttcc
    43561 ttggatttgt acacctgctt cgatcaatgt gtctggaccg atttctacgc ctgcatcgat
    43621 gtatgtcgtt gctgaatcga caaagcttac gccattgacc atatgcatct ggttaattcg
    43681 ctttctcatg atctcatttg ctttagctaa cgcgatccga tcattgactc ccattgattc
    43741 ttcaaaatca tccgtttgat aagcagcaac tgtatggcct tcttctttta agatttcgat
    43801 gatatctgtt agatagtatt caccttgagc attgtttgtt ccaactttgc ttaacgcatc
    43861 gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg atttcttgta caagagcttc
    43921 ttctggtgtt gcatcttttt gttccacgat tttttctacg atcccaatat ggtcacggat
    43981 gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc aagatcgttg cacttgcatt
    44041 ttttccttgg tgatattcaa aaagattgtt cagcgtttca gtagtcaata atggtgtatc
    44101 gccactgata actaacgttg ttcctttttt tccttccaag aaagaagcag cttgcaaaac
    44161 agcatgtcct gttcccaatt gttctgcttg taacgcatat ttactgcgtt ctcctagttg
    44221 tgctttaact tgttctgctc cgtgtccaac gatcgtaatc acttcatctg gtttcgtttc
    44281 actgacccga ttaatgatat gttctaccat aggtttacca gaaacgggat gtaaaacttt
    44341 atataatttt gacttcatgc gggtaccttt ccctgccgcc aaaataattg cataacgtgc
    44401 gtccaactgc gtcactccta aatatagttt gctgatttca gtttatccca gtatacccat
    44461 tttttcaaca aaacaagtga ttttctttta tcccagcgtt ttctggataa aaaaatagat
    44521 agaccataac gggcctatct atttggtaac cggtgctctc ctgccaactc ctcggtcaca
    44581 agtcacacta ctcataaaac atgaaaagct gttttttgaa atcgtttctt gtgacttaga
    44641 gttaaacggt aggcttacaa cctcttcatt aaacgttttt caatcagctg accttcggta
    44701 ttagctcaat aaacggcaaa atatgaggag caatttttcc gtttattgag ctaatactca
    44761 ggacaggacg ctgatttcac aacctctaga taaacggtgt tcaaaagcca gtttctcggt
    44821 attaagccaa atttatgaaa aaatgtggaa agccattttc acaaaatttt tcttactact
    44881 cgaaactaag cggctttttc acaacctctt attctgaaaa ataatttcct ggcacaaccg
    44941 taatattttt cgtttgagta tctacatctt tgacataaag cagtgacgta taatcatcga
    45001 tggcgcgatg accgttgaat tttgattcgg caaatacagt gatgccaaca agttctgatt
    45061 cgaattcttc aatcaagctc ttcattccgt tgactgttcc gccgcctttc atgaaatcat
    45121 ccactacaag gacacgtgaa ccacgtttca aacttctctt agacagttcc atcttttcaa
    45181 ttctttcaga agatccagac acatagttga cacttactgt tgatccttct gttatttttg
    45241 agtcccggcg tacaataacg aatggcgcat tcagataata ggacacagct tgggcaatag
    45301 gtacgccttt tgtggctaca gtcattacag catcgatttt ttcaccaagg tatttagaag
    45361 caatgattct tccaacttga cgcagcagtt caggttctcc taacaaatca gagagataaa
    45421 catatcctcc tggaagcaga cggtcttgtt ctgatagacg ttctgctaga gattcaatat
    45481 attttttcgc ctcttcatat gaaatttcag ggatgaacaa tacaccacct gcagcacccg
    45541 ggattgtttc caaggttcca taccctcgtt ctttaaatgt tttcttgatg attcccaaat
    45601 cttcactgat agaagattta gcagattcat aacgttcggc aaagcttgtt aaggaaatca
    45661 attcatgcgg gtgattcaat aaatactgtg tcatgtcaac tagacgttca ctgcgacgta
    45721 ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt ttacattata taagtttttc
    45781 ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag ttcggtattt attaaaataa
    45841 aaagaagcct ataaaaaatt tttcgttttt ataaggcttc ttttctttat aatttagtta
    45901 ttttttcttc tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca
    45961 ataaagacta atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt
    46021 cctgacagca tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt
    46081 ttgcctaatc gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca
    46141 ccggcaatcg ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac
    46201 atcaaatgaa cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac
    46261 atcggccgtt tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag
    46321 atgacttgct cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt
    46381 gctgaattcc ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt
    46441 aaaatggcaa tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc
    46501 acagctgcta atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat
    46561 cctaacgcta cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt
    46621 ctgatcacta aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga
    46681 aacgctcttc tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca
    46741 gtcggatctg tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa
    46801 ctgatttccc atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt
    46861 cttcatccat acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa
    46921 tacgcgctaa actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc
    46981 gcatatccca catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag
    47041 agagacgctt gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg
    47101 tactaggaaa acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt
    47161 tttttccttc ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc
    47221 ctaacgatgc tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa
    47281 attcacccgc atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa
    47341 aggtcaaatc agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg
    47401 atactttctt ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct
    47461 ttgatttgtt cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt
    47521 tcattcgaca atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg
    47581 atgttgtttt tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga
    47641 tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca
    47701 aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat
    47761 ttttgatcca gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc
    47821 tttttatctg gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct
    47881 aataccggat cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct
    47941 gattcttcat gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg
    48001 atcatcaagg tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg
    48061 tctgaactat tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt
    48121 ggttcgaaat catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc
    48181 cctacgatct cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc
    48241 tcttttttgt ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc
    48301 attaccgtag ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg
    48361 gaattttacg atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga
    48421 aatccatcaa tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt
    48481 tgctgctcat atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt
    48541 cctcagtttt tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata
    48601 aacttcctca caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg
    48661 acacaaggca aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt
    48721 gatcatacgc tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa
    48781 actgtttccc atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag
    48841 tccgtggatg tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt
    48901 agagacactc attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac
    48961 ttggatttct tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac
    49021 atctgtaccg acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca
    49081 taattggttg atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc
    49141 tgctactgga atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt
    49201 ttttagcaat aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt
    49261 atttgtttct acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa
    49321 gtctacgctc gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac
    49381 atccagtcct aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat
    49441 tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc
    49501 tgcctagtgg ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat
    49561 ccaaacaagt tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac
    49621 atttcctttt tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact
    49681 tcctttccta taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc
    49741 ataagcagac tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt
    49801 ttcttgaaag aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt
    49861 ggcttccgag tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc
    49921 taattcctca gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt
    49981 catcggctga gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc
    50041 gctagataat acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc
    50101 ttgaagccat ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc
    50161 gtaccaagta aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag
    50221 ccgagacaag tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg
    50281 aaaacccaat tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg
    50341 atagcctaaa caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg
    50401 attgccaact ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac
    50461 gacttgatct tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg
    50521 atggcttgta gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta
    50581 ctcactgcca agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag
    50641 caatgagatg cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat
    50701 aaaatgattt cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc
    50761 gtaaacgata aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata
    50821 aacgaatttt gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac
    50881 atataccaat atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga
    50941 tagtaaagca atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag
    51001 gccgagtaat aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa
    51061 cggcaataat acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag
    51121 taccatcatg atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg
    51181 gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat
    51241 gaacaataga agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag
    51301 ccaaactact gctacatctt gccgaacaga caattgactc attcctaaga tcatcaaaac
    51361 gtcaaaagtc cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat
    51421 gaacatagga tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa
    51481 aaataccgca agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc
    51541 ttttggataa aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac
    51601 tggtgctaaa aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga
    51661 ctgaataaaa aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat
    51721 aaatagtgcg acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata
    51781 aaaactagcc cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt
    51841 gtttgttacc cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt
    51901 ttctaagact ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc
    51961 agccagaatc atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc
    52021 ttgccaactc atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga
    52081 gccgaaaaaa atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta
    52141 tcgtttattt ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg
    52201 ttatcttatt tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa
    52261 aagaaagata tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag
    52321 ggaatatggc acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag
    52381 aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg
    52441 tccatgcatg caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg
    52501 tgaaaccaaa aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct
    52561 tattcatttc tattgcttca ggaaaatcat taatagattt agaaacagcg cttgaagatc
    52621 ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt
    52681 ctgcgatttg caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt
    52741 ttgaagcaat cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc
    52801 ttgctggcag ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg
    52861 tactgaatgg tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt
    52921 cttgtcaaaa attcctttca agtgataaaa atccatggga acttgtagac caagtttctt
    52981 ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag
    53041 caatcatcaa aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat
    53101 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
    53161 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
    53221 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
    53281 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
    53341 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
    53401 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
    53461 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
    53521 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
    53581 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
    53641 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
    53701 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
    53761 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
    53821 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
    53881 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
    53941 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
    54001 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
    54061 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
    54121 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
    54181 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
    54241 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
    54301 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
    54361 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
    54421 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
    54481 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
    54541 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
    54601 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
    54661 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
    54721 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
    54781 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
    54841 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
    54901 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
    54961 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
    55021 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
    55081 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
    55141 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
    55201 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
    55261 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
    55321 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
    55381 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
    55441 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
    55501 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
    55561 cgcgtcctcc aggagtgatt tctggattcg caaacatcac gcctactttt tcacggattt
    55621 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
    55681 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
    55741 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
    55801 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
    55861 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
    55921 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
    55981 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
    56041 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
    56101 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
    56161 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
    56221 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
    56281 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
    56341 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
    56401 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
    56461 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
    56521 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
    56581 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
    56641 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
    56701 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
    56761 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
    56821 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
    56881 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
    56941 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
    57001 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
    57061 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
    57121 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
    57181 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
    57241 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
    57301 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
    57361 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
    57421 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
    57481 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
    57541 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
    57601 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
    57661 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
    57721 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
    57781 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
    57841 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
    57901 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
    57961 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
    58021 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
    58081 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
    58141 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
    58201 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
    58261 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
    58321 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
    58381 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
    58441 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
    58501 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
    58561 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
    58621 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
    58681 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
    58741 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
    58801 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
    58861 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
    58921 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
    58981 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
    59041 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
    59101 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
    59161 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
    59221 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
    59281 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
    59341 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
    59401 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
    59461 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
    59521 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
    59581 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
    59641 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
    59701 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
    59761 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
    59821 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
    59881 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
    59941 cacggtgttc cagaagtagc ttcttcggca ataagccgaa at
//
LOCUS       DASJPH010000005        58126 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJPH010000005 DASJPH010000000
VERSION     DASJPH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.005"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="U3995_000266"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="U3995_000266"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1795618.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="U3995_000267"
     CDS             1708..2025
                     /locus_tag="U3995_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1795619.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2229..2594)
                     /locus_tag="U3995_000268"
     CDS             complement(2229..2594)
                     /locus_tag="U3995_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795620.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2802..3284
                     /locus_tag="U3995_000269"
     CDS             2802..3284
                     /locus_tag="U3995_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795621.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3481..4371
                     /locus_tag="U3995_000270"
     CDS             3481..4371
                     /locus_tag="U3995_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN1795622.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4696..7167
                     /locus_tag="U3995_000271"
     CDS             4696..7167
                     /locus_tag="U3995_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1795623.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7270..9006)
                     /locus_tag="U3995_000272"
     CDS             complement(7270..9006)
                     /locus_tag="U3995_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1795624.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9003..9707)
                     /locus_tag="U3995_000273"
     CDS             complement(9003..9707)
                     /locus_tag="U3995_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1795625.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9838..10341
                     /locus_tag="U3995_000274"
     CDS             9838..10341
                     /locus_tag="U3995_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1795626.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10301..10639
                     /locus_tag="U3995_000275"
     CDS             10301..10639
                     /locus_tag="U3995_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1795627.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10660..12078
                     /gene="ffh"
                     /locus_tag="U3995_000276"
     CDS             10660..12078
                     /gene="ffh"
                     /locus_tag="U3995_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1795628.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12190..12768
                     /locus_tag="U3995_000277"
     CDS             12190..12768
                     /locus_tag="U3995_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1795629.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12772..13293
                     /locus_tag="U3995_000278"
     CDS             12772..13293
                     /locus_tag="U3995_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1795630.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13372..13926)
                     /locus_tag="U3995_000279"
     CDS             complement(13372..13926)
                     /locus_tag="U3995_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1795631.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14179..14454
                     /gene="rpsP"
                     /locus_tag="U3995_000280"
     CDS             14179..14454
                     /gene="rpsP"
                     /locus_tag="U3995_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1795632.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14466..14717
                     /locus_tag="U3995_000281"
     CDS             14466..14717
                     /locus_tag="U3995_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1795633.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14842..15633
                     /locus_tag="U3995_000282"
     CDS             14842..15633
                     /locus_tag="U3995_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795634.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15803..16324
                     /gene="rimM"
                     /locus_tag="U3995_000283"
     CDS             15803..16324
                     /gene="rimM"
                     /locus_tag="U3995_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1795635.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16314..17075
                     /gene="trmD"
                     /locus_tag="U3995_000284"
     CDS             16314..17075
                     /gene="trmD"
                     /locus_tag="U3995_000284"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1795636.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17211..17558
                     /gene="rplS"
                     /locus_tag="U3995_000285"
     CDS             17211..17558
                     /gene="rplS"
                     /locus_tag="U3995_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1795637.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17690..18826)
                     /locus_tag="U3995_000286"
     CDS             complement(17690..18826)
                     /locus_tag="U3995_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1795638.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(18942..19508)
                     /locus_tag="U3995_000287"
     CDS             complement(18942..19508)
                     /locus_tag="U3995_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEN1795639.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(19644..20297)
                     /locus_tag="U3995_000288"
     CDS             complement(19644..20297)
                     /locus_tag="U3995_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795640.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(20347..20775)
                     /locus_tag="U3995_000289"
     CDS             complement(20347..20775)
                     /locus_tag="U3995_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1795641.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(20780..21154)
                     /locus_tag="U3995_000290"
     CDS             complement(20780..21154)
                     /locus_tag="U3995_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1795642.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            21458..21643
                     /locus_tag="U3995_000291"
     CDS             21458..21643
                     /locus_tag="U3995_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1795643.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            21656..21904
                     /locus_tag="U3995_000292"
     CDS             21656..21904
                     /locus_tag="U3995_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795644.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            21935..22105
                     /locus_tag="U3995_000293"
     CDS             21935..22105
                     /locus_tag="U3995_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795645.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(22207..22665)
                     /locus_tag="U3995_000294"
     CDS             complement(22207..22665)
                     /locus_tag="U3995_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795646.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            22833..23150
                     /locus_tag="U3995_000295"
     CDS             22833..23150
                     /locus_tag="U3995_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795647.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            23083..23562
                     /locus_tag="U3995_000296"
     CDS             23083..23562
                     /locus_tag="U3995_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEN1795648.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            23579..23755
                     /locus_tag="U3995_000297"
     CDS             23579..23755
                     /locus_tag="U3995_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795649.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            24012..24443
                     /locus_tag="U3995_000298"
     CDS             24012..24443
                     /locus_tag="U3995_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795650.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            24449..24766
                     /locus_tag="U3995_000299"
     CDS             24449..24766
                     /locus_tag="U3995_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN1795651.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            24885..25571
                     /locus_tag="U3995_000300"
     CDS             24885..25571
                     /locus_tag="U3995_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1795652.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            25546..26895
                     /locus_tag="U3995_000301"
     CDS             25546..26895
                     /locus_tag="U3995_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1795653.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            26886..27359
                     /locus_tag="U3995_000302"
     CDS             26886..27359
                     /locus_tag="U3995_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1795654.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            27334..27861
                     /locus_tag="U3995_000303"
     CDS             27334..27861
                     /locus_tag="U3995_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEN1795655.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            27876..30188
                     /locus_tag="U3995_000304"
     CDS             27876..30188
                     /locus_tag="U3995_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1795656.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            30435..30752
                     /locus_tag="U3995_000305"
     CDS             30435..30752
                     /locus_tag="U3995_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN1795657.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            30749..30910
                     /locus_tag="U3995_000306"
     CDS             30749..30910
                     /locus_tag="U3995_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1795658.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            30913..31212
                     /locus_tag="U3995_000307"
     CDS             30913..31212
                     /locus_tag="U3995_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795659.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            31212..31526
                     /locus_tag="U3995_000308"
     CDS             31212..31526
                     /locus_tag="U3995_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1795660.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            31519..32049
                     /locus_tag="U3995_000309"
     CDS             31519..32049
                     /locus_tag="U3995_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1795661.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            32046..32315
                     /locus_tag="U3995_000310"
     CDS             32046..32315
                     /locus_tag="U3995_000310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795662.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            32245..32448
                     /locus_tag="U3995_000311"
     CDS             32245..32448
                     /locus_tag="U3995_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN1795663.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            32445..32732
                     /locus_tag="U3995_000312"
     CDS             32445..32732
                     /locus_tag="U3995_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795664.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            33219..33632
                     /locus_tag="U3995_000313"
     CDS             33219..33632
                     /locus_tag="U3995_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1795665.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            34252..34416
                     /locus_tag="U3995_000314"
     CDS             34252..34416
                     /locus_tag="U3995_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795666.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            34828..35373
                     /locus_tag="U3995_000315"
     CDS             34828..35373
                     /locus_tag="U3995_000315"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1795667.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            35374..35691
                     /locus_tag="U3995_000316"
     CDS             35374..35691
                     /locus_tag="U3995_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1795668.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(35848..36012)
                     /locus_tag="U3995_000317"
     CDS             complement(35848..36012)
                     /locus_tag="U3995_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN1795669.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            36114..36635
                     /locus_tag="U3995_000318"
     CDS             36114..36635
                     /locus_tag="U3995_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN1795670.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            36622..37095
                     /locus_tag="U3995_000319"
     CDS             36622..37095
                     /locus_tag="U3995_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1795671.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(37102..37260)
                     /locus_tag="U3995_000320"
     CDS             complement(37102..37260)
                     /locus_tag="U3995_000320"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN1795672.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            37353..38114
                     /locus_tag="U3995_000321"
     CDS             37353..38114
                     /locus_tag="U3995_000321"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1795673.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            38334..39653
                     /locus_tag="U3995_000322"
     CDS             38334..39653
                     /locus_tag="U3995_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1795674.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            39669..40889
                     /locus_tag="U3995_000323"
     CDS             39669..40889
                     /locus_tag="U3995_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1795675.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            40924..41601
                     /locus_tag="U3995_000324"
     CDS             40924..41601
                     /locus_tag="U3995_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1795676.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            41598..42791
                     /locus_tag="U3995_000325"
     CDS             41598..42791
                     /locus_tag="U3995_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1795677.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            42806..43132
                     /locus_tag="U3995_000326"
     CDS             42806..43132
                     /locus_tag="U3995_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1795678.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            43136..43471
                     /locus_tag="U3995_000327"
     CDS             43136..43471
                     /locus_tag="U3995_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1795679.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            43468..43863
                     /locus_tag="U3995_000328"
     CDS             43468..43863
                     /locus_tag="U3995_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795680.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            43860..44231
                     /locus_tag="U3995_000329"
     CDS             43860..44231
                     /locus_tag="U3995_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795681.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            44247..44807
                     /locus_tag="U3995_000330"
     CDS             44247..44807
                     /locus_tag="U3995_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1795682.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            44932..45243
                     /locus_tag="U3995_000331"
     CDS             44932..45243
                     /locus_tag="U3995_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795683.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            45267..45458
                     /locus_tag="U3995_000332"
     CDS             45267..45458
                     /locus_tag="U3995_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795684.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            45458..49306
                     /locus_tag="U3995_000333"
     CDS             45458..49306
                     /locus_tag="U3995_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN1795685.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            49307..50179
                     /locus_tag="U3995_000334"
     CDS             49307..50179
                     /locus_tag="U3995_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN1795686.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            50176..51225
                     /locus_tag="U3995_000335"
     CDS             50176..51225
                     /locus_tag="U3995_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795687.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            51229..52053
                     /locus_tag="U3995_000336"
     CDS             51229..52053
                     /locus_tag="U3995_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795688.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            52071..54131
                     /locus_tag="U3995_000337"
     CDS             52071..54131
                     /locus_tag="U3995_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN1795689.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            54148..54297
                     /locus_tag="U3995_000338"
     CDS             54148..54297
                     /locus_tag="U3995_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795690.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            54294..54740
                     /locus_tag="U3995_000339"
     CDS             54294..54740
                     /locus_tag="U3995_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795691.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            54742..54879
                     /locus_tag="U3995_000340"
     CDS             54742..54879
                     /locus_tag="U3995_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1795692.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            54914..55156
                     /locus_tag="U3995_000341"
     CDS             54914..55156
                     /locus_tag="U3995_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEN1795693.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            55172..55369
                     /locus_tag="U3995_000342"
     CDS             55172..55369
                     /locus_tag="U3995_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1795694.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            55380..56396
                     /locus_tag="U3995_000343"
     CDS             55380..56396
                     /locus_tag="U3995_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1795695.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            56511..57719
                     /locus_tag="U3995_000344"
     CDS             56511..57719
                     /locus_tag="U3995_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795696.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            57703..57873
                     /locus_tag="U3995_000345"
     CDS             57703..57873
                     /locus_tag="U3995_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795697.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        19904 a         9803 c        12169 g        16250 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg gaatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga tagataaaat
     2101 gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa atgtatacat
     2161 gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg
     2221 gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca taacgaccca
     2281 aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa
     2341 ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta
     2401 gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca
     2461 gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca
     2521 agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag
     2581 caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
     2641 taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac
     2701 tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt
     2761 gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa
     2821 tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg
     2881 taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggatttgtgg
     2941 tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta ggtctcttat
     3001 taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag
     3061 tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg
     3121 agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc aaaccatggt
     3181 tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca
     3241 ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta
     3301 aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta
     3361 aagaatgcga aaaacgttta catgattttt acataactta actatatttt tacagcaacc
     3421 atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa
     3481 atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca
     3541 ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa
     3601 gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa
     3661 agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact
     3721 ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa
     3781 gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg
     3841 gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat
     3901 atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac
     3961 gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat
     4021 ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta
     4081 agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa
     4141 gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga
     4201 atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc
     4261 ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta
     4321 ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat
     4381 aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc cacatataca
     4441 tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga gctaataccg
     4501 agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata gaactgtcgt
     4561 agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat gtgaggaaca
     4621 ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga aaacgaaatc
     4681 aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca
     4741 caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg attaacggca
     4801 gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga tcccacattc
     4861 aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt gatctttctt
     4921 atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt tttaccagtt
     4981 atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa gatcttggac
     5041 gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat agaacaaaca
     5101 atagatatag aagaccttgt aatagatgac attgttattt tagagactgg acatcaaatt
     5161 ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt attgacaggc
     5221 gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag tttcattgtt
     5281 tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat ttcccagctg
     5341 acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc ttctcttaat
     5401 caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct tttttctcaa
     5461 agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg
     5521 atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc attaagtgcg
     5581 actaaactag caaagcaaag agttctttta cacaatatga aaagcattga gacattggca
     5641 agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa aatgtctgtt
     5701 caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca attggaagca
     5761 tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga agctgttaaa
     5821 gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt ttcttctgtc
     5881 caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc gcccgaaatg
     5941 gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc
     6001 tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt attgacagaa
     6061 gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga agcaagagcc
     6121 acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg agataatccg
     6181 ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca gtacgttgat
     6241 gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac agtttttgga
     6301 cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa caatacagta
     6361 gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata
     6421 gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct
     6481 gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag
     6541 cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca
     6601 gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt
     6661 actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg
     6721 aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta
     6781 gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca
     6841 gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg
     6901 ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc
     6961 atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg
     7021 agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat
     7081 ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt
     7141 ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac
     7201 aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt
     7261 tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact
     7321 gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg ctaaccctaa
     7381 tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat
     7441 ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc
     7501 atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt
     7561 ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt
     7621 ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat aattttgaac
     7681 gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc
     7741 ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat
     7801 gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc
     7861 ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa
     7921 atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat
     7981 ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc
     8041 ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag
     8101 ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac
     8161 cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc
     8221 aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt
     8281 agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg tctgatacaa
     8341 ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat
     8401 tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata
     8461 gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa
     8521 actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa
     8581 ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca aagtagtact
     8641 tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga
     8701 gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc
     8761 cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg
     8821 cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt tttttgtcag
     8881 gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca attgccagcc
     8941 accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa tttttttcca
     9001 tttcatcgtt ttggttcctg aaacttatag ccaaatcctc taatagtcac caagtacttt
     9061 ggatgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac atctacgatt
     9121 cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc
     9181 acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg tgttagttca
     9241 atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat ttgtccaatc
     9301 gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat ccgtctgaat
     9361 attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa atagtcgtca
     9421 gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt cagcatcaaa
     9481 atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc atccatcgtt
     9541 ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa ttctaatcct
     9601 tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt gaacgtcaag
     9661 agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca aatctccttt
     9721 aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc tattttacaa
     9781 aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg
     9841 attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa
     9901 gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg tccagaagtg
     9961 atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa tggattcgat
    10021 gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac ggaagcgtac
    10081 aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc
    10141 atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag tttttccgga
    10201 ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa aatgactgtg
    10261 ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa
    10321 tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac aaatgaacta
    10381 catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag aatacgaagt
    10441 aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg aggattatga
    10501 aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg aaaatatgac
    10561 tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg tacagcaaat
    10621 tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga aagcttaact
    10681 gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc agaagctgac
    10741 gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt taacttgcaa
    10801 gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa
    10861 agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac gatcacactt
    10921 ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat catgatggct
    10981 ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta tttaaagaaa
    11041 aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc tgcgatcgac
    11101 caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg aactgaagtc
    11161 agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa aaacgactat
    11221 gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga tgagttgaaa
    11281 caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc catgactggt
    11341 caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac aggggtcgtc
    11401 atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg ctcagtgaca
    11461 ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga agtattccac
    11521 ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt gattgaaaaa
    11581 gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat
    11641 tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat gggcccactg
    11701 gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag
    11761 gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac gccagcagaa
    11821 agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga
    11881 aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag aaaaatgatg
    11941 cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt tggttcaggc
    12001 gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa
    12061 aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa atgtctactg
    12121 ccggagcttt tctatcagca atttttattc tttactaata caattactct aaacgaagag
    12181 gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca atatcatgat
    12241 actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt gatgcttgat
    12301 attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc attcgatgaa
    12361 gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa
    12421 ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac
    12481 gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta tctttggtcg
    12541 ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt cccaacaaca
    12601 agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa atttatcgga
    12661 agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca ccttgacagt
    12721 tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa
    12781 atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac ggatgatctt
    12841 caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt ttatgaaaca
    12901 atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata tttggagacc
    12961 gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca attacaattg
    13021 ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct acaaatgaca
    13081 gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct attggaacat
    13141 caaatgattg atgaatggta tatccagatt acccctgtat tattagggga cgggattcct
    13201 ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac acgattcggt
    13261 gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta
    13321 tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt tttactctgt
    13381 taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc
    13441 ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat atgcttccgg
    13501 ttgctgcaag gttggcacat tcaaaaagac aagtgattgg cgtagatggt ggtttgcgcc
    13561 aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat gatcccaaac
    13621 actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg ctggcacaga
    13681 acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt cctcacgaaa
    13741 acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt aaaatggcag
    13801 atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg caaaagcttc
    13861 tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc caattttttt
    13921 tgtcatacga tttttcctcc ttcaaactat ctataataat cttacaaaag ataagtaact
    13981 ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac ttatttttaa
    14041 caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat cgaatttaat caagctgtaa
    14101 agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg aataaaacaa
    14161 tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg ttctaaaaag
    14221 agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg acgtttcatc
    14281 gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt aaaagaagac
    14341 ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg taacatcctt
    14401 tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa ataaggtgac
    14461 cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta gtcacttatc
    14521 ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac aatctaacag
    14581 tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct aaagcaatcc
    14641 gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg aatatcatcg
    14701 atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag cttttttgtt
    14761 ttttttatta attgaagaaa tgattatttg gcaaaacaag caagaaagcg tatccttata
    14821 ggaggaggga gtgaacagcc aatgagaaag aaaatgttat ttattccatt tgctctattg
    14881 ttaagcgctt gtagttcggt tactcaagaa tcagacaata cacatctatc aagcagtata
    14941 tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa cggcttccac cactgaacaa
    15001 acaatggcta cctcaaataa tgagaaaaag actgctttag accagttgaa agaacaacag
    15061 ccaaatgttc ccatgcctct agatgtacca gtctcttcag gctatctcaa tatcgcagcg
    15121 acacatacaa agcaaggtta ttctatttta tattacagaa cagatcgtcc cctcgggttg
    15181 aatgctgacg aattgaatca agaaacgcct attgcaacct atctatatca atatgggttt
    15241 gcttctagtc aagaaacaat ccaagttttg cagccatttg agatcgacac aaatggtcag
    15301 caagtggact taggttctcg aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt
    15361 cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg ggaataatat agaaggccaa
    15421 gatcccttac tattagctaa agaaatcgta gcttatttgg aagaaaacag tctaccagca
    15481 cctgaacaat ttggtaaaat tactgttgat atgggtgata cgacgaatcg agccgtagaa
    15541 gtaagctggc aagaaccgaa aaatgcctat acgatcactc atcaagatcc tatgtctgcg
    15601 ttgaagatgg cagtttctat gaagaggttg tgaaatcagc gcgctgatct gagcccatca
    15661 tttaactcca agtcacagga agtaatcaaa aatagcacgc cgcatttttg cataattttg
    15721 gcttaatacc ttaaaagaaa actagaattg cctcttcaca catgctaaaa taaaacagac
    15781 aaaaaagaaa tggggatata agttgactga atacttgaat gtaggaaaaa tcgtaaacac
    15841 acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg gatttcccag aagaacgtta
    15901 caaaaaagga acggtgctaa cactattcca agacggaaaa gcacctgtgg aattaacagt
    15961 caaaagtcat cgcaaacata aaaattttga cttattgagt tttgaaggac atccttctat
    16021 caatgatgtg gaaaaatatc gggatggcat attaaaagtg tcaaaagaca actctgtaga
    16081 tctagctgaa aatgaattct attaccatga gatcatcggt ctgcatgtat tagaagatgg
    16141 aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt
    16201 tcaacgtccg aaaaaagctg atatattgct tccatatatc gcttctgtcg tcaaagaagt
    16261 cgatttagaa gctggagtgg tccgtgtaga aattccagaa ggactgattg acgatgaaga
    16321 ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg
    16381 gtaaggctgt agataaagga ttattagata tcaatatctc taacttccgc gattattcag
    16441 ataacaaaca tcagacagta gatgattatc catacggcgg tggagcaggt atgctgctca
    16501 aagtccagcc catctatgat aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa
    16561 aaagagtaat tcttcttgac ccagctggta aacgattcga tcagaaaatg gcagaagaat
    16621 tttcacaaga agaacactta gtattcattt gtggtcatta tgagggatat gatgaacgta
    16681 tccgcacatt agtgacagat gaagtttcct taggagatta tgtccttact gggggagaat
    16741 taggtgcgat ggtcatgatc gatgcaacag ttcggctttt gccagatgtg ttagggaatc
    16801 agacttcagc tcaaacggat tcttattcaa ccggtttatt ggaacatccc caatatactc
    16861 gcccggcaga atataaaggg atgaaagtac cagaagtatt gacaaatgga aatcataaat
    16921 tgattgaaga atggcaatta aaagaatcac taagaaggac ctatcaacgt cggcctgatc
    16981 ttttggaaac acttgaatgg acacctcaaa tgacgaaatt tttagaagaa atcaagaaag
    17041 aagaacaaga aaaaacagca gatgaagcca agtgactttt tctaaaatct tctttacagt
    17101 aggttgataa tatgttagta ttttatggtg agtgcaaaag cacttaacta ttacgatatt
    17161 ccgctgtgag aaaagctcat aagaatattt ggaataagga gaagaaaacc atgaatccat
    17221 taatcgaaga attaacaaaa gaacaattac gttctgacat cccagcgttc cgccctggtg
    17281 acactgtgcg tgttcacgca aaagttgttg aaggtactcg tgaacgtatc cagttatttg
    17341 aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga aacttataca gtacgtaaag
    17401 tttctaacgg agtgggcgtt gaacgtacat tcccattgca tacaccacgt gttgcaaaaa
    17461 tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac
    17521 acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc atcgttatca gacagagaaa
    17581 gaacccttgg tattcaaggg ttcttttttt attatctgtt tttttatatt aacgatttct
    17641 atttggtaca tttttgatta ataaaaaggg gcaaaaaagg ggcaacaagt tacagttttt
    17701 ctatatcttc tttcaatttt tcttttactc cttttgtcac atgcaaataa attttttcag
    17761 tgatatcact attttcgtga ccaacgcgat cctgaatggc atacagagga gttcctagtt
    17821 ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga actaagtttt ttatcaatct
    17881 tcatgtcagc tttatgactt ctcaaatagg tgttaattgc tgttagttgg aaaggagttc
    17941 ctttcgttgt ttgaaataga aattgaccat ttggatttaa ttctagaagc tcattgtaaa
    18001 tagctatcgc tttttttggt aaatcaattt ctcgcattcc ggcagctgtt tttgtagaat
    18061 cagatttttt catatcagct attgaacgtt ctctatacat catcgtccca tttataacta
    18121 ctgatgcatt attattggta atgtgtacat catctttact taatgcgatt gcttcaccag
    18181 acctcatccc agtcaaatat agccactgga aaaggagaga atatctttta ttgtgtgaag
    18241 ttgtgaaatc tactagtcta ttatattcat catcttctaa aaatttatct ttaattttta
    18301 tcgtttttga ctctctttta taatcaataa ctacttcatc gataggattt ttttctacat
    18361 aacctttttt catagcatat gaaaaaagaa gattcaattt ggatttaatt acactgacgt
    18421 acttatttga caagtcatct ttatatatca tgtcttcaaa aatattattc aaatctattg
    18481 tattaatacc agaaacgatg taagtctcag gtatcttctt ctttatagtg tttaatatat
    18541 tattcgtagg ataatatgta gattccttaa cttgtctttt gtatataact aaccattctt
    18601 caacaagctc atgaaatgtt agatcaggct tatgaagtgt tttttcgttt aattttatat
    18661 caattttttt atttaactct agcaatgcca ctttctgagt ttctcgagat ttatttttat
    18721 aagtaatgct cacttttttt ctttttctag tttttggatc aatatatcgc tcattatatt
    18781 tatatacttt ttttccgttt ttatcagttt tagtttcgat ccacattgtt ttcatctcct
    18841 atttgttgct ataataggaa tagataagta agccgattat agcaggttta tttttcatca
    18901 cgtccacaaa ctttggcgag ggaggggcgt gtttttgtta gttattgtgg taggttatct
    18961 aaagcgtatt gtgcttgctc atttgtaaaa ccttcaaata taagttgatc gtacaattgc
    19021 gaatctgaca ttgatgccca atcatcgtaa ctttccgctt tagccaaagc ttgttcgttg
    19081 taatcgacat caacattagc cagcgcataa tcgattgcat cacttggata ttcttcaaac
    19141 tctagttgtt cacgcaaacc ttgttcagac ataccagccc aacctagata gctttcagct
    19201 ttgctcaatg catttctgta ttcacgagga acactttctt cttgagtaac ttcttctata
    19261 gtagtttcag tagtttcagt ttcttcttca acgactgtag attctgctac cgattctgca
    19321 gaagacactt ctgtcacgct ttctttagtt gtttcagcga cggtattttt agcatcatca
    19381 ctgcctcctt gcgaacctat agcaataaac accacaacaa ccgctaacaa ccaaaaccat
    19441 acccgtttat aaaacggttt cttaacttta tacattttcc catcttgacc cataactttt
    19501 tttgccattt aaatatccct cgcttcttgt tataatatat ttgtgatctc agaaatgagg
    19561 tatgagtccg tgttgcagca cgggcttttt tctttataac ttttttagag attataggca
    19621 aaatagtagg gcataaaaat gtattattga attccgtatt tagaaaatcc taattgaact
    19681 tcaccggaag tcttttgctg tgttgtacgc aatgcttctt cttcagacat tccattctgt
    19741 actttccatg caacaggcga catcccgtat ttgttaacaa aatcagtaag tgataaagtg
    19801 tcagcgtctt gctgagcgct tgtttgttgg tcttctggat tctgttgaga tgctgcttgt
    19861 tgttctttct gatcttgact gataatattg ccagcatcat ctgtagtcaa tccattttca
    19921 taaagggcca cgccgaaagc ttcccactct ttgttggacc aatttgcacg atcagctgga
    19981 gttgactgta aagtgcgttg tttcatctgt tcatatgttt cttcttgagg tgcggtttgg
    20041 attgtactct gactggagct tataactgtt gggctaggtt ccgccgtagc ttggttggag
    20101 cttgtaactg ttgaactaga atctgtttta gatgtagatt tgctagtaga tgaactggtt
    20161 tcagttgttt cttttgtttt acttattttt gtttcttggt tagaagtggt atctgttgat
    20221 tcagcttttt tattatttga acaagctgaa agtagcagag cagtacttaa caacaacata
    20281 acgctaactt ttttcatttt ataattcctc tttctcgttg taatatgtgt gctaacacgg
    20341 gcttttttat ataagaaaac gataagcgct ttctggaagc ccgtaaagat tctttaattc
    20401 ctcgattttt ttaggatatt gatcattatc ttctttataa agagaaacaa tgagattagc
    20461 agcaaagcaa ttagcttcgc tttcagattt gcttctagat gttcttgttg atacataata
    20521 gctggataag ccacgatgaa aaatagcgtg acctaattcg tgagcgcaaa tgtagaatct
    20581 ttcctcagag tctcgcagtt catcatttaa aaagattatc gcacgatctc taatttcttg
    20641 aaactgcccc ttgggattct cgataaaagg aacgtattga attttaatgc ccatcttttc
    20701 acaaatataa aaaggattag cggactggta tttccgcttc aactcctcga ctaaattaat
    20761 cgtatccatc tccataagct cacatctttt tgcctttttc tttgtcttct ttcacaatat
    20821 cccagaaagt cgctatcagg atatctttta cgcgctgtat ttgttcgggt gtcaatgttt
    20881 ccccaccata agacatatta acatttgagt ctagtagttt atcaagttca accacttcct
    20941 cttttgtagc ccacttggga acattattat ttcccaataa ataatcagtt gtgacaccaa
    21001 aataatcagc aactttcttt aagttctcag attttggcga ggctttatcc catcttctta
    21061 tttgtccatt agagatgccc acctgtcttt ctacttctgc tatagtcaca tgcttttcgt
    21121 ctgctaattc tttaatctta gtaactaaac tcattattat caacctttca aagctgaaaa
    21181 gaaaataaat agcttaaaag ttatattttt ggttgacaat tagcttttaa gctagtatat
    21241 ttaattcgta agctaaattg ttagctaaat aagagcaaca aaaaactcta ctaatttaaa
    21301 acattctctc ggtcgccaaa cttagaaatg ttattttaga ggctttttat aagtcttatt
    21361 taactatgta ttcataatag cttaaaagct aataggtgtc aacgatttag ctaatttttt
    21421 agcttacaaa ttatttgttt agaaaggagc tatttttatg tctgagaatt tagacttaaa
    21481 aattcgagcg gagatgagaa aaagaagaat gactttcaaa gaactagctg cgcttatcgg
    21541 tatttcagga gcttatttat cagatattct aaacggcaat cgtgatggaa agaaagcaca
    21601 acagcatatc gaaacagtga aaaaaatatt ggacatccga tagggggggt aggcgatgac
    21661 aaaactaaaa aaacaagatt ttgtaaaaaa atataattat tctccatcta cttatcaacg
    21721 tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac gggtcacagg
    21781 acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca aatcctataa
    21841 caggttacgc acaagaaagg taacacctaa agaatttatc gagaagcatt tagttgattt
    21901 ataaaaaaca gaacattttg agaggtgaag gttaatgggt aaattcaaca gagcattagt
    21961 attcagcgca ccgctaatcg tctatgcttt agggctttgg ggaagtagac aagcgttgat
    22021 aggaacgatt gtgtacatgg tttggatttt tatagggctg gatgaagctg agtacaaaac
    22081 aaaaaagcca gtcgggaggg actgactaat gagtaaggac gatattgtat ttctgttaat
    22141 tatgttttgt gggattggat caataataat taatttgtat ttgattatta aagaaaaatt
    22201 tttcaattaa aatgagtctt ttagctttaa cttaaatttt ttttgataat tagctgttct
    22261 tatgaccatt acaagttttt caccaggtaa tatatctttc atatctaaaa cttctgaaaa
    22321 agcgactgag tagccaggag agattgcttg tgccaacggt ataggagata aaaggctact
    22381 tttatgaatg cttcccatat caaaaccatt ggcgtgataa tgtgaacaat taattttctt
    22441 accgttgtaa agatacgtac attcgataat tgagtttggc atttttgatg ggtttgcaat
    22501 tacgccagta agaatcattc gattacttct atcatgctta actaaaaaat aggagagcgt
    22561 aatttttctt ttatctttaa ataagttata gagggataaa aaaaatcctg aaatagaaat
    22621 gattaaagta atagcgactt ggtaatcgct caaaattttt atcaagtaat tcaccacctt
    22681 tttactcaaa ttatatcaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa
    22741 aataaaaaaa gacacgaacg ccggcaagca tgatcgtgtc agggaaataa actatctttc
    22801 tatattttac cacagaaagg aatgaaccgt aaatgctaat tgcaacggat acactagacc
    22861 gcatcttttt aaaagacgaa tacaaactgc gcaatataga tgcgtcagga attttagtat
    22921 ttgatcttta tgacaatgga aaaattggta tctatcaagc aagtgatatt gaagaaacaa
    22981 accttgcttt cgaacaaatt gatgattctg tggaattgga tttagatgag gcaatcctag
    23041 cttttgaaca aattgcaaaa ttattaaagg aggcacaaaa gaatggcaac tctttaccaa
    23101 ctcagcgagt catatatcaa agtcctggaa ctggcagaac aattggatga agaaattctt
    23161 cgcgatactc tcgattcgat taatgaagcg atcgagtata aggcagaaaa cttagcaaaa
    23221 atagttaaag aagtagaagg gaaagctgag ttaatcgata gtgaaattaa acgtctgcag
    23281 gaacgtaaga catcgctttt gaacaatgct aagagtatca agcactattt acaagaggaa
    23341 atggaaaaga ctggtaaaac gaagattaaa ggtgaattat tcaacattgg aattcaaaat
    23401 aatccagtat cggtcaatgt aatcaatgag aacttaattc caaaaggatt ctttacccct
    23461 gtgcctccca aattggataa aaagcaattg aaggaggaac tgaagcacgg agatattcct
    23521 ggtgctgaac tcgtccaaac aaaaggtttg aggataagat gatgaaggag gagaaataat
    23581 ggaacacgaa aacgtaaaag acgctttgaa agctgctatc gaaatcgcgg aagctaaagg
    23641 aataaaggtt gacggcaaac cagcaactgt ccatgacatt caaaatttga cgaaagaaca
    23701 tctttatttt atcgccgact tgcttggttt gtcggaattg tatttagaca aataacccca
    23761 cacggaagga gtggctaggg tggttgtatt acaagtaata gtctgcgtaa cattcatttg
    23821 gtcgtgcata ccggtttgta tatatatcat gaagaagctt cgccctgagt cttacaaatt
    23881 atacgcaatt tacagcataa cagtagctct tgcatttaca ttgtttgcaa tttctaggct
    23941 tccgttttaa tcgtgggaat gaatttttaa tgaactaaat tatctataaa gcaaaaaaag
    24001 gagaaacaac attgaaaaac aaactagcaa aaacaacagc aatcatcgga ctagcactag
    24061 gtagcggcgt tatcggctac gcagcaagca atgcgtttca agatttggac acgatcaaag
    24121 caaatttcaa cacagtccta caatacggtc aaacgaaatc gcaacgtgtg tcagaacttg
    24181 aatcacagct atccaacaac actcgcacac aggagcagct gaaagccgaa attgagcaaa
    24241 tcaaatcgga caagcagaag gaaattgaag ccaagcaacg agaagtcgaa caaaagcaac
    24301 aggagatcgc tacaaagcaa caggaagccg atagcttgcg ccaacaacta tccacagtgc
    24361 aaaacgacaa ggagcagtta gaacagcgcg tgagcgagtt acggcagtat acggatcaaa
    24421 aggtagggga gttggggaaa tgaaagagtt gactatttat gacccaaata tttcatgggc
    24481 taaacacaca attaaagttt cttttatgat ttggggttac aaaggatatg ttacttacaa
    24541 agtgggcggt aacactaagg gtttatcatt aatagctatt gattcagatg atctatatga
    24601 tgctaatttc gaggataacc cagttaattt ccgtgacctt gatgaagatt ggttctctat
    24661 ggaacttact aacgataaag gcgacagtac acttgtagaa gatgaattcg acagactagg
    24721 tgattatatt gtcggtgttg aaattattgc gcacgaacca gagtaggaga gtgaatacaa
    24781 aatgattaaa agaatcacta tatcagttga agaagatagt ggatcaacaa tgcagcatac
    24841 cgacaatcga aaaacttttt taaccaatag aaaggagaat tttgatgcag attaagaagg
    24901 cgacggatat cgaacaaacc aagggaacat atttgatcta cggtgctcca ggaaaaggta
    24961 aaacttcaac ggctaaatat tttccaggga aaacactgat ccttgatatt gatcggactt
    25021 caaaagtcct tagaggtgag aaaaatgtcg atatcgtata tatcgacaac gaagatacct
    25081 ggaacgactg gggcaacacg ttggctgatt taactaccaa ttatgtaggt gtttatgaca
    25141 acgtggttgt cgataatgtc agcgaattgg aacggtgtat tctgtccagt ttaggcgcag
    25201 aaggaaaaaa caacggcgtt ccttcgcaag gcgattatca atatatgcaa tttagaatgg
    25261 tgaacagcct acgctatatg aaaaatttag acagcaacct tatctggacc gcatgggaag
    25321 aaatcgattt gtggacggat tcggatggat cttcttacaa catcgctttg ccgcagatca
    25381 atcgaaagat tcgaaacaat attttgggat tatgtgacgt tgttggtcga ttgatggtaa
    25441 aggaagacga tgaacgcgga tttattttac aagccacaga ttcaacctat gccaaaaatc
    25501 aattggataa tagatctggc tgtaagcaaa gtgagctgat tttagatgga attgtacgac
    25561 taccagaatg atttagtcga tcgtgcaaga caagcctatg ttgatggtta caaggcacca
    25621 tgtatcgtat caccgtgcgg ttctggaaaa tcagtcatgg ttgcagagat cgctaaacga
    25681 accacacgaa aaggcaacag agtgttgttc ttggtccatc gtcgtgaatt gattgatcag
    25741 atcaaagata cgtttcaaaa gatgggcgta gataatcagc ttgtaaattt cggcatggtt
    25801 caaacagtcg tacgacatct taaaaccatc aagaaaccac aactgatcat cactgacgaa
    25861 aaccatcacg ggttagcagc gtcctacaga aaaatctatg agtattattc ggacgttccg
    25921 cgattaggtt ttaccgcgac acccatccgg ttaaacggta gtgggttggg ggatgtgaac
    25981 gacttgctaa ttgaaggagt atcggctaaa tggttgattg agaatcatag attagctcct
    26041 tatgaatatt atgcacctaa attgatcgat acagccgaat taaagaaagc ttcgactgga
    26101 gattttacaa agaaatcaat ggataaggca gtgaagaata ctatttatgg cgacgtgttg
    26161 aaacattata aaacgttagc agaaggtgaa caggcaatag catactgtca cagtatcgat
    26221 gcaagtaaac acactgctga aatattcaac gatgctggtt ataaagcaac acatatcgat
    26281 gcaaaaactc caaaagatga acgtgcagat attatcgaag cgttcagaaa tcatgaaatt
    26341 aaaatcctat gcaatgttga tttgatcgga gaagggtttg atgttcctga ttgttcgact
    26401 gtaattatgt tgagacctac tcaatcgtta tcattgtaca ttcagcaatc gatgaggggg
    26461 atgcgttacc gaccagataa aacgtcaatc atcattgacc atgtgggtaa tgtgaaccgt
    26521 tttggattgc ctgatatgga tcgtgaatgg agtttagata caaaaaagaa aacaaaaact
    26581 gatagtgatt tatctatcgt tcaatgcgcg ttttgttttg gtgcttacga acggccaaaa
    26641 ggcgataata tttgtccgta ttgcggagag ttgcagccga ttgaagaacg aaagtcagag
    26701 ttagaaatcg atgaatcggc tgaattaatg aaagtcggag aaacgaaaat tactttgaat
    26761 tttgagaaca ataaatatta caacatgacg gaagatgaag cagaaagtat agaggactta
    26821 tatgccatag caagagctaa aggatttaaa cctggttggg cttatatggc tgctaagagg
    26881 aaggggtggt tgtaggaatg ggcggtattt ttggtaaaaa agcagaagat gttcaaggtt
    26941 caataataaa tggcgtaaca ataataggtg atactggaaa aagagatagt tcaggcaatc
    27001 aaattgtaat cgctagaaat aaaaataacg aagtttttga atcttctcta tctaatataa
    27061 aaagaggtgt tgttaacggg acaggcgaag agagtggcta cgttaattta aaaaaagcta
    27121 atgaaaaata ccatgttgaa ggaacttacg ttaaggcttt gtctgtcggt ggaacaaaag
    27181 gcgttaattg ggataaacga agaaataaat ggaaagcacg attatatttc aaaggagaat
    27241 atgtcttaca aaaggatttc aacaccaaag aagaagcaat ccaagcacga cttgcagcag
    27301 aagaaaaata tttcaaacct atcttagaaa aatatgaaaa taaaaaggag aatgattaac
    27361 atgacaggat ttaacttaga cttttcaaaa gcgcaacaag ggaacgaaat caaagacggt
    27421 acgtacgaag tggtcgtaaa caaagctgta gaaaatgcaa ctaaatcagg cgcagaattt
    27481 atcgatatcg atttgatcgt tcgtaacgat gtggatcaac cgttccaaaa taaacacatt
    27541 ttcgcaaaaa tctggaaagc caaagctacc ggcaaatata acgaaggtat gatcatggca
    27601 attgcacaag cgctacagtt ggaagacgga aaatcatata acgggtttga tgaattgctg
    27661 gcggactttg ttttgaaaac cgcctcagta cgcgtgaaaa ctgaagagtc aaatggatat
    27721 aaaaacgtca atgtgaagtc ttgggataaa acgaacacac ggggcgtaat gaatcaccaa
    27781 ttcaaaaatg gtgatgaacc atccttcgga ccggaacgtt caagagcgac aacggttaga
    27841 aatgacaatc ttccatttta attgaggtgt aatcaatgta tgaaaaaatt ccaaaagagc
    27901 ttaaaaatct caaacaatgg tgtgtttata agctagtttg ggatgaaaag cggcagaagc
    27961 acacgaagat cccttataac gccaacaacg ggcataaagc caaatccaat gacgagagta
    28021 cctggtctga ttttcagacc gctctcgctg ctattgatca ctacggaatg agtggactgg
    28081 ggttcttttt caaaccacct tatttcggca ttgatatcga taatgctgaa ggtgaagttg
    28141 agcgatttaa atcaggtgat atagaagaaa atattatcta tgaatttatc gaaagcatga
    28201 gatcctatgc tgagtattct caatcaggta ctggcatcca cattattgct cgcggagaat
    28261 tgcccggcgg aagacgcagg aaaggtgacg tagaaatgta ccagaacggc cgattcttcg
    28321 ttatgactgg aaatgccgca tcaaaatatt tagaaataac tgagccgaat ccaaaggata
    28381 tcaaacgcct atatgatcgc tacgttggag atgaaaaaat catccaattc aaagaagaaa
    28441 atccgctgat gaataccgtt gatttaccgg ttgaagaaat tatcaaacgt gctgaatcgt
    28501 cttcccaagg ggcaagattc aaagtcttta tgaacggcgg ttgggaagct ttttattcct
    28561 cgcaatccga agcggatatg gcgttcgcca acgacttggc tttttggacc ggtagagact
    28621 ttgaaaaaat ggatgagatt ttcagagcgt ctgcaatgat ccgtagcaaa tatgaccaaa
    28681 aaaggggatc ggcaacttac ggagaatctc tactaaataa ggcaattgct gatacaaatt
    28741 ccgtctacaa ccccaaacgg aaatctgatt ttaagatttt tatcaaagac caggaacagc
    28801 caaaagagga aaaatactat tcgtacgacg acaccggcaa cgcggatcgg tttactgata
    28861 tctacggaac gttagtcaaa tattcgtaca ttgataaatc ttggtattac tacgatggga
    28921 aagtttggct acaggacaat accggcgaag ttcggaaaat gatcgatacg acagtagaca
    28981 tcatgggaaa agaaccgtta acgatccctg aaggcgcaga cgatgaaacc aaagaagctt
    29041 tgatgaaagc gaaagaaaaa cacgtcaagc gttctcgcag caatgccgga aaaaacgcta
    29101 tgatggacga attgaaacat cgactatccg tattgccgga agagttcgac aaagataaaa
    29161 cgctgttcaa cacacaaagc ggctatctgt ctttgcatga cggtcttcta cacgagcatg
    29221 agatagacaa aatgtttaca cgtgtttcaa atgtcgagta cacagaatcg gtcgattgtc
    29281 ctatgtggga agaatttatt aatcagattt ttgacaatga tcaagaatta atcagatata
    29341 tccagaaatg tgtcggatat tccctgaccg gatctaccag agagcaatgt atgttcatcc
    29401 tctacggtca tgggtcaaat ggaaagtcgg tatttttgga aattatttct gaattgatgg
    29461 gaaactatgc gatgactatg caggcacaaa cgatcatggt caagcaaagc caatcatcag
    29521 caaacagtga tattgcacga ttgaaaggtg cgcggttggt cacttcgtca gaaccgaatg
    29581 aaggcgtccg actggacgaa ggtctggtca aacaattgac tggtggtgat aaagtcacgg
    29641 ctcggcacct ttatggaaaa gaattcgagt tcgaacctga attcaagttg tggctggcaa
    29701 cgaaccacaa gcccatcatc cgtgggaccg atgacggcat ctggcgacgg ttaaatttaa
    29761 ttccattcac tgtccaaatc ccggatcaca aaaaggataa aaatctgaaa tttaaattgc
    29821 agacggagtt gcaagggatc cttaaatggg caatcgatgg ttgcctgttg tggcagagag
    29881 agggcttgga gaaaccaaag tcggttgttg cagcgagtca ggattatcga aacgaaatgg
    29941 atcaaatagg tacatttatc gaaacgtgtt gcgaaacagg tccaggacta aaaatatcag
    30001 gcggagaatt atacaaagtt tatcgtgaat gggcatcaga taatggcgag catactttca
    30061 ctaacacgaa gtttggcaga gagatttcaa agaaatatag caaagaaaaa gctggtggat
    30121 ttatggttta taaaggaatc acattaaaac caagaaaata cgataacgtt agagaacttt
    30181 ttaaataaat ctggagggtt ctggagggtt gagaacgaaa ccctccaacc cttagaaacg
    30241 ttgatatatc agtatttata ttatattttt ctttctttct ggagggtttt aaaaaaataa
    30301 taaagtatta aagtaaaaaa taaaaagtaa attatataaa aaagaatagg tttttgggtc
    30361 tgaccctcca aactctccac caaaatcttc ctaaatgctt tgataccaac gattaaacag
    30421 tctggagggt tacattgaaa tcagaacatc aaatacaaga cgagatcaga attgccttat
    30481 caaaacatgg ttgcacgatt tttcgatcca atgcgggaca agtgacaacg atcgaagggc
    30541 ggcaatttta cgggatgcca aaagggttcc cagatctatg tggacatcgt aattctgatg
    30601 ggaaagcaat ttatatagaa gtaaaaaatg aacgcggtaa gttgcgtgaa gatcaaaaaa
    30661 ggtttgctga atttttaaac ggtcaacctg tactatacgg tgttgcacga tcagtggaag
    30721 aagcattgaa aattgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
    30781 tcgaagcctt cagcgtgaga ttgacgatct ggaacggaca aaggcagtga tggtcaatga
    30841 aacggcgagg aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
    30901 ggaggaatga gcgtggataa gaaagcagca atgaaacgaa tcatcgaact gacacattct
    30961 gagaattggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
    31021 tggagtgaaa aaaccaaacg gagaacaccg agaaaaattg caatctggca tggtgaccga
    31081 attctagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
    31141 atctgggata gagctaaacg cgagaacgtt gattcgaaag gtcgtcaatt taaacattgg
    31201 gagaagaaat aatggaggaa ctaatcacaa aagtagagca gtgggcaaaa gataagggat
    31261 tggatcaagc tgattccagc aagcaaatgt taaaaacgat cgaagagatt ggggaagttg
    31321 ccgcttctct agctagaaaa gatgaatatg gtttaagaga cggaattgga gacgtagtag
    31381 taaccttgat tattttagct atgcaaaatg atatggattt gtacgagtgt ctgaaccaag
    31441 catacaacga aatcaaagga cgcacaggaa aaatggtaga tggtgtattc gtgaagtcga
    31501 gtgatttgga ggacagcgat gaataaaaaa gtattaattg ataaacaagc attgattgat
    31561 gaattaatga aaatacctgg tgtgggatct aatagtgacg ctttagaaac gattaaacgt
    31621 ttcccatcgt atgaaccgca gaagccagtt gtgcctaagt ttgtggcgga actactcgac
    31681 tattatcgtc agtcaacaga tgttgattta ttagctttat tgataacttt ccatgattgg
    31741 tattatcgca aaactaaaga cggtgagcat gaagaagcaa ttgattggct agttgatcat
    31801 cctgaaattt atatgcgtgc atggctgttt ggctacgagc tcgagaaaga gccgttgtat
    31861 tgcgtaataa tcaatggtag atatcttgtc aaagtattca gcaatacaaa cgttgtcata
    31921 ttagttccag cagatgaatt ttcacagtac gttactcaaa aatttcaatt aactgaaaaa
    31981 caaatcaaag caattgatga aagatactgg ccgtttgctg tgccagtgga tgaggtggta
    32041 gaaggatgag cgacccatac attactaaat tagaaaagtt gtccgaagat tttgagaaga
    32101 ataaaaataa actaactaaa gataaactta tagaactggt taggttggtt atcagaggag
    32161 ctattacaag agaaaaggcg cagcaaaaca tagaaatttt tgctcgtagt gtccgagatg
    32221 gattagagaa gaaagttaaa caaaatgaac aacagacacc gcagaataac aaaactaaga
    32281 aaacgggaac tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa
    32341 gaaacatata aagtggcaag tcagtgtgtt gctgatgcga gcggcgctat tcgtaagttt
    32401 ggaatttcga tattaagtga tgatcgtaaa tgggaggaaa aagaatgaaa ctaaaagacg
    32461 gattttactc cagcagtcat ggtatcggcg gtttaatgct agatatgccg acaaagaacc
    32521 ctaaaacacg tgagaaacca aaattcaaag tcggtgacat ggttcgctgt gaagcagaag
    32581 gattcatcta tccattttgt ggatatgtag aacatctcta taatcactca gcaatcattc
    32641 gtattgaaaa cacgatggaa tgtgacaagt ggttagcgaa aagcaaagag aatttagcag
    32701 tagcgagatt ggtggatatg gaggttatat aaacagcgaa aaaatgtact tctaagagaa
    32761 aaatttattt tttataaaaa taggaaagta tcattatgaa aaagaccaca gagataaata
    32821 aacctaccac ataaaaaatg aatatttctt gcgattcaat ataggtatat ttaacaattt
    32881 gtacaataaa aaatactata ggtaaaaaag ataacatgag gagcttattt gttctagagt
    32941 gtttgacaaa gtaacaaaat gcgacgatac caatacaaac gggaatccaa aataacagca
    33001 tgctccacat agtcactcaa ccatctccta tacgctttat tatcaataga taatatcatt
    33061 tttcgtaata aagataaaaa aataagtaag gcttctcatt aaaatataaa aaagacagcc
    33121 gaccactgac tgcctatata agagtattga aataaaaagc tgctgatata ataaattcca
    33181 caagtttatt atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag
    33241 atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc
    33301 ggatggcagg tcgctctttg atagatatta agtcgccgat tattacggat atgccgaagg
    33361 cgccgaagca cggcaataag gcagaagacg caatcattca gatgatggat atagaagcgg
    33421 agagagacgc gattctagca gccttgatgg ctcttagtct gattagtcgt cagatactct
    33481 actatagctt ttgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt
    33541 attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat
    33601 ataaacacgg aagaataatt gcttataaat aatttggcgg ctttttggcg gaatgatggc
    33661 ggtttttagc tatttaccag tgatattatg gtagtgtcga aagattagtg ataggtctaa
    33721 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
    33781 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
    33841 ttatctatca tccgttgctg tctattgttt ttttaattat tcacacgata actaaaggtg
    33901 agcgaagaga aatgattcca ttaataattt caatttttgc gctttgtctt aatgtctata
    33961 tgattggatt taaaaatggg caaaataaaa gatagtagta actaagaaag aaatttttat
    34021 attgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca
    34081 actatgcaat gttcgattca ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa
    34141 aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacatat
    34201 tagatcactc gttgagtggt ctttttattt tacataaagg aggtaacaac gatgtataga
    34261 ccacaatact tagaacagaa gtatgaagta atcactgtgc aaaacggtaa cggtgagata
    34321 gtacgaaagt atagaagacc aataaagagc gatacatata aacgaaagga aaacaatgaa
    34381 gttatttctt ttcgcagaag gagaaaagcc aaatgagaaa ctactggtat gtatcgctaa
    34441 ctaatgaata tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa
    34501 tcaaagggaa gtactctatt gtcgaaatgc taagagaagc tacaccaaac gaagtggata
    34561 aatgcaagct gatatattgc ggtcatggct attggaaaga cgagtatatc caacaaaaca
    34621 ttgagaggtg gatagataga tgagttacct cgaacatttg aaacgttgct acatgcattc
    34681 taagaataaa cttcctgaca gctacactgt agatgatgtt gctatacatg tattgaagac
    34741 tgagagccat agcagtcctg atggtagcag taaggagcag acgctcgcgt ggttcaagtt
    34801 ctttaagtgg attaaggagg aagagtaatg cacatgcaca taggagaaaa gatatataaa
    34861 ttgagacggc ttaataacga aacaatgaac gacttagtta aacaaactgg agtgtcaaaa
    34921 tcatggttat ctgatattga gtctggaaaa aagcaacgcg ttgattttaa taagatatgt
    34981 gcaatagcca aacattataa agttagctta gaatactttc gggatgatta tttggatagt
    35041 gatgaacacg aggatattat tcctttgaca ggcgaaacaa gagttaaatc agtgaaatcg
    35101 cttgaacaaa gcctaaagta tctgaataac aaacaagaac ctaaccttct agagatcaaa
    35161 ctaaaagata ctgactcagt accagaggtt tggtacaagg gtgagaggtt ggatgaattg
    35221 cctaaaggat tagtagatgt ctcgtatcat tggaagactg atgattttac taatgatgat
    35281 agaggagcga acgacatcac gattcaatac ttttctagct ttaatgataa gtatccagat
    35341 aagaaaacaa tcggacacaa gagagatatg taaatgaaag aagttagacc tagagacgaa
    35401 atagacaaac tatacaagac caaacgatgg cgagacctaa ggcaagtagt aatagctagg
    35461 gacttcggca tgtgccaaga gtgcaagcgt cgagggcgga acacaagggg cacgatcatc
    35521 catcacatag tcgaggcgag ggaagacctg tcactgttct ggtccgtaga taaccttgaa
    35581 tgtatctgtg tagcttgtca caacagagag catccagaga gatcaggcgg gaagaagaaa
    35641 ccaaaaccta aatcacatat cgttaaaatg tattcaactc ctgaaagata agtttgcagc
    35701 gaaatgaagg tagcccccct actctaaaag attaaagagt aaggcttgag aagaacggtg
    35761 ctgtccttcc ttcgtaaaaa gaccgctttt caagtttttt ggagaaaaag gaaaaagccg
    35821 accaatttaa gccggctttg gacgaagcta tttcttagtc catttgtttc ctttttgaga
    35881 agtagggggt aatcggtcgc ctggatcaat ttttacttct tgtccgcctt ggacatttcc
    35941 accacgagga cccacttctt tgtaggttcc ttttggtttg ttgtcttcgc cgggtttata
    36001 gagttctccc ataggaatcc ctccttaaca aatttcggca cagcactgcc gataacctaa
    36061 ttataaggat tgtgataacg atttcaatct atcttttgaa agaaggtgat attatgccgc
    36121 aaccagcgaa gagtgcaaaa ttacaattat taaacggaaa cccaaataag aagaataccg
    36181 aagaactccg caagcgagcg gccgcagaag acaaattaaa aatggctact gacaaaatca
    36241 aaccgccgtt atggctagat tcgctaggaa aggatacctt tgagtttatc gccgatgaat
    36301 tgctgtctgt ggatttaatc agtaatccgg acgtccatac aatggctcta tactccaatt
    36361 ggtattcgca atacgtttct ttagaaaaac agcttcgaaa actacaacga gagtacaagt
    36421 tgaactatgc gcttgcgaaa aaggaggcag aggcgagagg tgagccgttt aatgaaccta
    36481 atgaattaat tggtaatccg ctctctcggc agatggatac agcgtctcgg aatctccgtt
    36541 cttttggcgc tgatttagga ctatcaccag cagccagagc taagttagct attaagatgg
    36601 ctgatgatgg tggtgatgac gatgacgact tctaatattt tggatatgtc ttacacagaa
    36661 cgtgtggact attggcaaag ctatcttgag gagcaagctt cttggggtgg ctttttaaaa
    36721 tgtccatatc cggaattgtt aactacttgg tatgcggaac gattaatcga tggaagcata
    36781 ccagccagca aagaaaatat tcaagctgct aaacggcata tgcgtgattt gcagcgccaa
    36841 ggaacagatg attttccttg gatctttgac gaagaaaaag gtcaccggcc tattagatat
    36901 atcgaaaaaa aatgtaaacc aactgaaggc gactttggtt cgtttgtttt gcaaccttgg
    36961 cagcatttca taattggatc catgtacgga tgggtacatc gtgatacagg agagcgtcgc
    37021 ttccgcgagg ctcttatttt tgttggacgt aaaaacggta agttttgccg tttaaaaatc
    37081 gggcaaaatc ggtgaaaacc tttatttttg gctttctttt agtaataatt cgatagcttt
    37141 atcaagcaat ttagacatag gaaccatagt ttcttccgac atttttttta atccttcgta
    37201 taaatcttta tcaattgcgt tagaaattct aattctgttt tttagtccgt atttgtccat
    37261 gtgtgacact cctttttatt ttactataac atccgattcc accgcttgca actaccatca
    37321 attgatggta gtatataaaa gaggtgagta gattgaaaaa atacattatt tacaaacact
    37381 taaataaaat aaatggcaaa atttatatcg gtgtcacaaa tgatattggc agaaggtgga
    37441 ggagcggtgg gattgaatat aaaccaccaa agaacgaaac ccaacaccac agaagttttt
    37501 ggaatgcaat acaaaaatac gggtgggaaa attttgatca tttgattatt gaagaagact
    37561 taacgatgaa agaagctttt gagaaagaaa agttttatat tgaactttat gactcaacta
    37621 ataagaaaaa aggttataac atcgcaaaag gcggaaatgg cggaataata tataaagttc
    37681 accccaaagg aatgctaggg aaaaaacaat ctaaagaatt ttcttcaaat cacagtaaat
    37741 gggcaaagaa ccataagaat aattgtatga caaacggtga tgttgtatgg ggtgttactc
    37801 acgaacaccc aaaaggcatg ttgggaaagc accacagcaa agaatcaatt atgaaaaaga
    37861 aagcatatag tggtgaaaat gctgtaacct caaaaccaat tgtcgcaatt gaacgggatg
    37921 gaactaaaag agaatttcac agcgctaagc tttgcatggc ttactattcg attagtacat
    37981 gtgtttttta ccgactttta aaagatggcg ccccttatgt gataaatcct aaagcgaact
    38041 acagaaataa agaaaaaata ttagctatcg aaggaattat gttcaaacat aaagaagata
    38101 ccgaggtaag ttagtagatt gcgaaaggtt actaactacc gtaacgcgta ggagttgaat
    38161 aaatataatg ctcccaagag tgtccgacag cagataattt gctgaaaatg tacgctaaac
    38221 tgggccagaa ctgactgacc gatgaaaatg agggtgacct ccagagtgcg agataaaaag
    38281 ctcgcagata ataacaatcg aaaacatcac taatttcagg cctttccaca tacatggtcg
    38341 cttatgatga tgaacaaggc gccaacgttt acgtattggc aaatgctcgt gatcaagcaa
    38401 gcttgttgtt tgataaggcc gcagaaatgg tcaaacaatc gccggcgctc tttaagaaat
    38461 ttggtaagcc taaacgatca agtattaatt atgctcccgc cttttctaaa atggaaccac
    38521 gcgcctcaga tagccggaaa ttggatgggc taaacactca ctttggtatt tttgacgaga
    38581 tccacgagtt tacgaattac aatctgatca acgttatcaa gaaatcaaga ggaaccagaa
    38641 aacagcctct gatagtttat atcacaactg ctggatatgt attagatggt ccgttgatgt
    38701 cttattttga gcaaggtgtg gactgtttgg aacatttgga agatgacatc gatgaacgga
    38761 ctttctatta tctggcaaaa cttgacagtg cggaagaggc tgatgaccca agattatgga
    38821 tcaaagccaa tccgaatatt tgtctaatga attttgttgg catgctagat gactatgtta
    38881 aggataaaaa agatccaaaa gaatatgctg actggattac caagcaattt aacttgtttt
    38941 ccgatatcga tgagctgtca tttgtcgata tgcctaccat taaacgaaac aataaaacca
    39001 tcgatattga aacgctcaaa ggtaagaagt gtgtcggtgg ttttgacttg tccgaaacgg
    39061 aagactttac cgcagccgtt ttagaatttc cgcttgaaac aggcgaggta ttcattttgc
    39121 aacacacatg gatcccacaa gctagatttg atcgagataa caatcaagag cgtatcaaag
    39181 cgtgggagaa ggtgggagat ctaacgatta ttcctggtga ttacgtcaat tatgaatacg
    39241 tcttaaattg gtttgtagaa aattcgaaaa tctatgacat tgtaaaaatc aattatgaca
    39301 aggccaaggc gctacgatta aataaagaac tagaaaatgc aggatttgaa accaacgaga
    39361 ttcggcaagg gtttctatca ttaggtgggc caatgcaaaa cttcaaggaa atgctattgg
    39421 acggtaaggt gattttcaac aattccaagc tttaccgatg gtatctatcc aacgtcaagc
    39481 tggtgatgga tcgcaactca aactggatgc cgtctaagca gtccaagagt agaaaaatag
    39541 atggttttgc agcaagtttg aacagccacg ccgaagtgtt gaatatgttg gttaatcctg
    39601 tcggaaccgg gaaagtaacc tattactcga tttccgattt aatgaatatg taagaaaggt
    39661 gtggaggaat gagtatttta gatcgtttgc gttcttttgg ccgagcgaag ccgaaagcga
    39721 gcaaacaaga gtattttttg aatgacccgg gattgatacc gtatttagtc ggaaaagatg
    39781 aaatatcaga agggattttt tccgtaatta gccgtgtatc gaacgttttt gcgtctctcc
    39841 ctctcaaaat gatagatgtg gagtttggcc aaccggacga ctgtcctgca tacaacttgt
    39901 tgagcgaagg ccctcgatat tttacaaagt ttgatttttt ccgggacgtg gaagttttga
    39961 gaaactacca agggaatgcg tatgtgcaga ttttccgaaa tatcaatgga gaagtagcag
    40021 atatggcgtt agtaaaacct ggtgcttgcc atccagtgat tgatatggat agcggggagc
    40081 tttactacca agtaactgcg actgacaaag gcagttacaa gcaagttatc tatgtacatt
    40141 acatggaaat gcttcacttt aaacaaccga ggtttggcgg cttggaaggt acagacccca
    40201 caaaagtatt aacgaatacc ctcggatatg atcgagaagt ccgaaaaatc tctttaagtc
    40261 agcttaaagg aagtaatgaa gggctaaaag ttaagtttgc tagcaatatg gatgaagaag
    40321 ctaaaaaagc tacagttaaa aacattgctg atttttatcg acaaaacggt ggactacttg
    40381 tggaagaaaa cggtgtagaa atcgaacgtt tacaacgaga gctggtagac agcaagcttt
    40441 tagatactga taaagtatct cgctccagaa tcgctatggt ctacaatgtg ccggagcatt
    40501 tcatcgggaa taaccagtcg agttactcct ctcaggaaca gctcaatatg gaatttttga
    40561 cgtacaatct agtaccgacc gttaatcaat atgaagcgga actaaataag aaaacactat
    40621 cgagaaccga aaaagcaaaa ggttatcgat ataagtttaa tgtcgcaagt ttgctaagag
    40681 ctgacactca ggcaagggga caattctatc aaatcatgcg acgaggcgga gcatactcag
    40741 cgaatgatgt ccgaagattt gaggacttgc aaccgataaa taaaaccggt atggatgatt
    40801 accatatttc cggagaccta tatccaatcg atatggatcc aacattaaga aaaacaacct
    40861 cgtctaaaag cgtagccgaa aacggttagg tttttttagt ttgcaccgaa gggaggtgga
    40921 aaaatgaaaa aagtgacgtt aagcggcgat gtcgtggata acgataccgc gtggctttat
    40981 gactggtttg ggatcaattg tatctcacca gggaaaattt ctgccgctct tacagaagca
    41041 gcgggggatg aagtagaact tgatatctca tcgaacggtg gggatgtcct agcggcaagc
    41101 gaaatatata ccgctatccg tgcctatccg ggaaaggtat ccggaaatgt tgtgagtatt
    41161 gcagcaagcg ctgcaagtgt aatcgcttgc gcttgcgaac cgcttagaat ctcacctacg
    41221 gcacacatca tgattcataa cgcatgggtg accactagtg gcaacgctga ggaattaaaa
    41281 gccaatgcag aaatgttaag cagtgtggat gagtctattg ttaatgctta cgagatcaaa
    41341 acaggactcg atcggaaaaa acttgctgat ttaatggcga aagatacttg gttaaatgct
    41401 caaacagcag tagcagaagg gtttgcagat gaaattatgt ttgcagaagc accagtaacg
    41461 gtactcaatg cctctcaacc ggttattcca aaaaacgcag taactaagtt gaaaaattta
    41521 atactcaaag cggaagcacc gcaagaagaa acactcttac agaaaaaact aaaagcctta
    41581 aatggaggga aaaacgaatg aatttagaac aattaaaaaa tgcgtgggtc gaggcgggaa
    41641 gtaaagtctc tgacttaaat gcacaactca atgcagcatt ggttgacgat gaaaaaacag
    41701 aagaagatgt agtaagtttg caagcacaag taaaagcagc acgggctaaa cgggacggat
    41761 tgaaagagca agtggcaaat atggaagccg aacaagtctt aaacgtcaaa aaagaaccat
    41821 tagataaaaa agatgaaaac ttgaaaaaca agtttatcaa agactttaaa gcgatggtca
    41881 atggtgatcc tgctattatg gctactttga catctaatac ggatgaatct ggtaatgcta
    41941 tcggattgac tattcctgta gatgtgcaaa cgactattca tactttggtt cgtcggtttg
    42001 actctttgca agaatacgta aacgttgaaa aagtgaccac taccagcggt tctcgcgttt
    42061 atgaaaaatg gtctgatatt acaccgttga ctgctttgga tactgaagac ggtgaaatcc
    42121 cagcaaatga tgatcctgca ctttacttga tcaaatactt gatcaaacgc tatgcaggta
    42181 tttccacagt aactaacagc ttgctaaaag atactgccga aaacattttg gcatggttgt
    42241 ctaaatggat cgcgaaaaaa gtagttgtta ctcgcaatac aaaaatcttg gcagctattg
    42301 atggaatcaa agcggcacaa aagaaagatg ttacagatgt tgatggaatt aaagatatcg
    42361 taaacgtcca acttgatcca gctatcgaag ctacatctat gtttattaca aaccaagatg
    42421 gcttcaatgt tttagataaa gtgaaacgtg ctgatggatc ttacttgtta caaaaagacg
    42481 tagcttctgc aactggatat accttcttag gtaaaccaat caagaaaatt gcttctcgtt
    42541 tcttgccaaa caaaggtacg caagctactc ctaaatatcc actgtacatt ggtgatctga
    42601 aagaagccgt tacattgtat gatcgcgaaa acatgagctt gctgacaacg aatattggtg
    42661 gtggagcttt tgaaacagac accactaaag tacgcgtcat cgatcgcttc gatgtgcaac
    42721 tagttgatga tgaagcggtt gttttggcta cttttacaac tattgcgaac gagacaccgg
    42781 cgaaagttta aggagctgat tccttatgat tcttgatcct aaaatggatt taggcgaaat
    42841 caaaaacgca ctaaagattg ataccgatga tgacgatgtg gaagtaagcc gtgcggcaca
    42901 agctgcaatt gcatacatta gaggggctat cgggaatgat aagccctctt tttataagca
    42961 agaaaatgaa acggttgatc tgattaattt agctatcctg caattagcag atcattatta
    43021 caacgctcga tcagcaaccg tgagtgggaa cttgcgagag tacgatttag gttttacaag
    43081 cctaatcttg caactcaaag caagttattt gctttttgtg gaggaggagt agcgtatgcc
    43141 ccttatccaa acaggaaatt taaatcaacg catcaagttt gtccgagata cgacggttaa
    43201 ggatgaggac gggcaagttg tcccgacttc tacaaccatt cttacttgct gggcaagtgt
    43261 gcagacacaa cgcctgaacg atattaagac gtcgattggt acggctttgg aaggaacact
    43321 gacgttcatt atccgctacc aacaaaaatc agagctgacc aatgatatga aagtgcgttg
    43381 gaatggaaaa acgtttgaaa tcattacgat tacgaaaggc gagtttgcga aggacttcac
    43441 gacaatcatt gcaaaagagg tttcaaaatg agtgtagaag tcgatgcaac cgaagtgtac
    43501 aaagcgctta gggaagtaaa agcaaacgtt caacgagtgg aaagcccagc acttagaaaa
    43561 gctggggagt acgctcaaga agagttacga caaaacacac cttactggga tggaacgaag
    43621 tcaaacggta aacgtggttc gtatatgcaa gaacatgcta agaaccatgt ggttacaagc
    43681 tcgataaaaa acggattggt agaagtcggc tatgacaaag atgtttcttg gcggatgcac
    43741 tttatcgagt tcggaacaat caaacaacgt ccaaaaggtt tcgtacaaaa aacacaaaag
    43801 caaatcgaaa aacaagtaac acaaatcatt gctgacgaag taaaaaggag gctaggactt
    43861 tgaaaacggc agtatcacaa gtctattcaa ttctgaatag caatgaaaaa acaaagaaca
    43921 ttgattttta caccaatagt gttccggaat cagctcaaac agtacctagc cttccagttg
    43981 gcagaattac agagatatcc ggcaactatg aagattttgc aagcaacaat cctttgacca
    44041 ttcaatttaa cgtacaggta gatgtatggg tgtcaaccat gaaagaggtt gatgcctttt
    44101 attttgccct tgatgaggtt atgaggggga atggttggca atgcgcatac acggaacaaa
    44161 cagatgacga ggacttggaa ggtgcaaagc ggattatcaa acgatatgta gcaaatattt
    44221 cactaaatta aaaggagaga aaatagatgg caacagtagg attcgagagc gtcatttttg
    44281 gcgtaaaaac aggtgcaggc ggcactctaa aagaattagt agcagataag tcgaaaggcg
    44341 gagcgatcga agctaaaatt actggattag gcgcaacttc taacacaaca tacgcttcaa
    44401 acgtaccgtt cttcattgca agtaaagggg tttcgtcgcc aaaagttacg cttgacgtgg
    44461 cagacttaat ggataacggc atttacagcg aaatcattgg tgctaaaacc gtggatggtg
    44521 caaatgtaat tggttcagaa actgaagcgc cttacgtgtc ggtagtcatg gttacagcga
    44581 acaaagaagg aaaacgcttg ttcatgggat tgacaaaagg aaaattcagt catccagata
    44641 tcgacatgaa aacagctgaa gacaaagggg tagaattgca aaccgattcc atcgaagggg
    44701 aattcatttc tgatgaacgt ggctatgtat acttaacagc cgtagaatca gaaggaatga
    44761 ccttacaaaa attcaaggac ttggtaaata acaaagaggg ggagtagtta accctgcatc
    44821 tacaccaacg acagatacag ggacaccaaa agaaccagaa ccaaaaattg atacacaagg
    44881 ttagccattt ttggctagcc ttattttttg taaaaacaag gaggaaaaca aatgattgaa
    44941 ttgcaattga aacttgacgg aaagaaaaaa acattcaaac aacaagatat ttccgcacgt
    45001 gcaatgcgtg agtgtatcaa attttacgag aaagcggaaa aagcagacct aactgattta
    45061 gaagcgattg attcaatgat tgcaattaca gcagatattt tccaagatcc agcagttaca
    45121 tttgatgcta ttttagacgg tttgactgcg agcgagttag taccggcatt agaaagtgtt
    45181 tttgaacaaa taaatgaact gggaaacaat gaaaaaaagc agacggcgag caaaaagaaa
    45241 taagtttttc tgaagctagg aaagcaatgg atcaaatcta caaagattta atcgaagcag
    45301 gttggacgat gagagatgtg gacgaagccg actatcatta tttgttacac ctttttggag
    45361 aagtggagag tggcgaagaa tatgtagatg gtgctgattt catcaaacaa tttttatcgg
    45421 ctgaagactt agtaaaactt gaggaaggag gtaaataatg gcaggaaaag gacaaccggc
    45481 aggaaatatc aagctaggga ttagtttaga tagcactagt tttggtaaca cgctggacga
    45541 aatcaatgcg aaagtcaaac aagctgagtc gaatatgcgt gccaatctaa aggcttatga
    45601 ttcagcagga cgttcatacg aagcacttag tcaaaagacg aaagacttgt ctacggttat
    45661 ggaagggcaa aacgccaaag taagagaatt aacaaagcgc cgtgatgaag cgattagcaa
    45721 gtatggcgag gaatcgaaac aagttgctaa ccttaacaca cagataaaca atgctaccgc
    45781 aaaatataat gcttacagtc gccagttgaa cgacacaaaa aaagaattgg tgtattccaa
    45841 aacagccgtc aatgatttat ctaatgaaat caaagaaaat gaacgacaaa tgaacgccga
    45901 agtaaaagcg ttgaaagccg ctggtgatga atctggtgct tttgaagcaa aacaaaaagg
    45961 gctagccaaa caaacggaat tatccgagaa agctatcgaa gaacagcgca aagttgtgaa
    46021 actgatggct gatgagtttg gcgattcagc aaatgaaacc gaagatgcaa aaagggcatt
    46081 agaaaagtta gaacgacaaa gccaaatatc tagcaggcaa ttagaagcac tcaaaagctc
    46141 cagcgatcaa tcaggaaaag aaatagaaga ttttggcgac aagtccacaa ggtcagctag
    46201 gaaactggac ggactaaaag acaaattaag ctcgctaaaa agcgcatttt cgtttggtgc
    46261 agttgctgga ttagcgcata acgctattag cagtgtagta agtggcgtgc aaggtttggt
    46321 tggcgaagca gtaaacgcat cggattcatt gatgaagttt tccaaaacca tggagtttgc
    46381 taactttggg aagtcacaga tagaaagctc gaaaaaagaa atgaaagact acgccgataa
    46441 gacggtttat ggtttagaag aaattctgaa cacaaccgca caattggcat ctaatgggat
    46501 tcctaactat acagaactaa ccaaggcggc aggtaacttg aatgccgttg caggcggttc
    46561 tagtgataca ttcaaatccg ttgccatgat gctaacgcag acggcaggag ctgggaaact
    46621 aacaactgaa aactggaatc aattagcaga tgcgataccg ggtgcttcag gattgttaca
    46681 agacgctatg ttgaaaaacg gagcttatac aggaaacttc cgtgatgcaa tggcgcaagg
    46741 tcaaatcact tccgacgagt tcaaccaagc aattgtacag ttaggtatga atgacggagc
    46801 agttaaggca gccacttcta cagacacatt gagcggttct tgggagcaga tgaaatccac
    46861 tgtaataaat gggctacaaa gtattataga aaaaataggc gttgaaaata tcactggttt
    46921 catcaacaga gtaacaaaag ggattgaaaa ttctattcct aaaattactc aatttatagg
    46981 ttggttgaga gatattggaa cgtggatcgt tgaaaataga gagccactaa catggattgt
    47041 cggaatcata ggcggaatta cattagcagt aaaagcattg aacgtagcaa gtatgttgct
    47101 ggcaattact ggcggaacat tggcagcccc ttttgtggcg attggtgtag cattaggcgc
    47161 actagcaggt gctttggtga tagcttatac aaaatctgaa acatttagaa atatagtcaa
    47221 tgcggctttt acagctgtga aaaacgtagt tatgagcgtt atcaataatt tggtggaata
    47281 ctacaaaatg ttgtggagcg tgttgcagtg gctttgggaa aaaataaaag aatgggcttc
    47341 atggattggc aataaattca ttgaaatgaa gaacagcgtt gtgaatacgg ttcagaacct
    47401 atggaatggc gtgaaaaact tcttcagcaa tggcgttgga gatacgtgga ataaggtagt
    47461 cggttgggta aaaaacattt tcaacaaagc aactgaattg aagaacaacg tttctgatgt
    47521 aatcggtaac ctgtggaatg gtatcaaaga cacattccgt agaggtatcg atacggtatt
    47581 caattggttt tcagaactac caacgaagat gaagaatgcc attattggcg gtaaaaacgc
    47641 cattgttgat gcgttcaaaa gtattttcaa cgcagcactt aaagcgatag gtaaaccagt
    47701 taacgcaatc atccatggag cttcatgggt actagaaaaa ttgggcgcag aacctctaca
    47761 agaatgggat gtaccacaat acgctacagg tacaccagca ggtgggcacc caatcaatgg
    47821 tccaatgatg gttaatgatg gacgtggagc agaaacagtt atcacaccag atggtagagc
    47881 cttcattcct aaaggacgca atgttgttct aaacgcacca aaaggaacgc atgtcttgac
    47941 agcagaagag accgcgcaac ttcaaggttc taaagctcct aagtatcgtt acaaaaaagg
    48001 tactaacttc tttggtaaca tgtgggatag tgtgaaaaag gttgctggta atgtaggtca
    48061 cacaattaaa aacgtagtag gtgacgtgtg ggactttatt tcagacccgg gagcgttagc
    48121 tagaaaagta cttggggggt tagatgtatt aggtggattg acaaagtatc cattagaagt
    48181 aggtaaaggc atcctatcta aagcaacaag tgcactgact gaaaaaatca ctgggttgtt
    48241 ctcatctggt aacttagata cctccgtagg aacaaatggc gtctataaat atttggctga
    48301 tgttgctaag tctgtgatga agaaattccc aggatttgtg gcaactagtg ggtatagacc
    48361 aggtgaccct tattcacatg gtaaacgtaa tgccattgat attgcattac caggcgtcac
    48421 aggaggctca cctagataca cagaagcagc aaactacgct tttgacaaat tcgcttccaa
    48481 aattggttac gtaatcacta atgggaaagt tcgtgaccgt tcaggacaat caggtcaacc
    48541 agcaactggt gcatgggagc catggcccga tggagatcac tatgatcatg tgcatttaaa
    48601 cggtgtgaaa gacccacaaa acactcaaat ttcaggagat agcgtgggag gcagtggggt
    48661 agaaagatgg cgcaatgtag caattagagc gttgaaaatg accggtcaat acagtactgc
    48721 aaacttaaat gcattactaa atcaaatgcg tacagagtca aatggtaatc ctaatgcagt
    48781 taacaattgg gatattaacg ccaaaaatgg aacaccatca aaagggttgc tccaagtgat
    48841 tgacccaaca ttcagacagt atgcaatgcc aggattcaac agcaatattt ttgacccact
    48901 atctaacatc ttagcttcaa tcagatacgc actatcaaga tatggctcac taacaaatgc
    48961 ctatcgtgga gttggttacg caaacggtgg aattgtaaac caacatcaaa ttgcggaaat
    49021 cgcagaagga aacaagccag aaattattat tccgttagat aaggctaaac gatcaagagc
    49081 gatgcaatta ttgtctatcg caatggacaa actaggagtt tctcctaaaa cttatggaaa
    49141 tgcgacaact gtttccagtg attacgaaat cctagaagcc attgaaaaac aggcggcaac
    49201 aacgaatcag ctgttatcgt tattgctggc atttttcaaa ggaaatagcc gaactgatag
    49261 agatttagct ttagatattc agaagatctt ggttaggagg atgtagatgc gaactgtatt
    49321 actgagaaat agaataaacg aagaaattga tttgtccaca gaagactatt ttgctactgg
    49381 gttgagtaat atggggtttg aagtaaaaaa agaacatgtg ggacagtggg ggaatttcag
    49441 agaaagcagt gagagcgttg aaatatctga atttcagtca tctgtaatca tttctgtgca
    49501 tgggtttcga gaaaaagaac tgtacaattc acttgtgcag ttcctatcgg aaggtccgtt
    49561 tgaactggaa tttgcttttg acggcgaaac aatggtaaga agatgcagtc taaaatcttt
    49621 aagtaagact gaaatcgatc cgaaaacatc gttactaaca gacactttag aactatattt
    49681 cacatcaaac tggtattccg taaaacgcga aaaattaatt cagcgaccga atgtagtgaa
    49741 aacacgtggt aaggtttttc cgtataaaag atcttatatc tacactcaaa acttgtggga
    49801 gaaaaaaggt gtattcaaat tcaataataa ttctgtatac ctaacgaata gcaaggaacg
    49861 gatgtccccg ctaaaaattc gtgtgattgg gaagtgttca aatccgtatt gggaagtaat
    49921 ccaaaactca caaatcatcg caacggatgg atacttcata gatatgactg aaacacagac
    49981 tctagaagta tcaagccttt ttgaagatac aacggcgatt ttaaaagata ttgcaggggt
    50041 agaatcttct gtctaccaac aacaggatta tacaaaaact aattttgttc aggccccaac
    50101 tggagaattc agcattgtgt ttcatgttgg gggggcagac gtagagattg agttatacga
    50161 ggagcgtgat ctgttttgat tttagcagtc acgctttttc atcgtgattt aatgctttat
    50221 aacgaacatt tattctctaa cagttttgaa tttggtgtgg acgagatcaa cgaagaggct
    50281 agtagcttta cgatggataa gtacgttcct gtaaaaacag gcgattttct actagcaaaa
    50341 tatattccta gtggaaaatt tgcatatttt ggtgtaatta cgtcgcagga agacgaaaaa
    50401 atcagttgta aaagcttact tagtttagct gatagtgaga taccgactgc acgtgtgtca
    50461 ggagataact acgaagagca tatccggcgg ttgattgaat actatttgct gaatgatcca
    50521 acgaaacaac taaaagatat tttagacgtc aaagcagaaa gtgcgacctc tcattcgtat
    50581 caagctaccg atacgaataa acacaagtta agtgcgtata ttctcaatgg tttcaaaaaa
    50641 tacaacgtaa aatggtattt caagggaatt caaaacagaa aaatttatac aggcatacgt
    50701 gctgtaaatg aatcaattta cattaaagac aattcttctg aatttagcga ttgggatgtg
    50761 tttgttcagg cgccgggcgc tggaaacgag aacaagctat taatcgttga taaagcaatg
    50821 aaagatatag agaaaccgat aatactgtca acatggtatt tggacgaaga aaacaatttg
    50881 acacaagatg gatcgaaaga aaatatcacg aaaccaactg tcaatttggt taatatctac
    50941 gatcaaaccg cagaagataa agcatcctac gaagatgtgg caaaatcaga gttgaaaggc
    51001 aatacgtatt cgcatgaaat caaagtgaac gttgtaagaa atgcaaaaaa tttgaatgtc
    51061 gaaacgattg aaacagggat gtttgccacg atttcttata aaggaaagat atacaagtcg
    51121 gttttgacag cttggcgaat atcaagtgat aaggagtttg tggaattgac tttcggaaac
    51181 ataagaagtc gttttatgga ttattttgaa gataatgggg gataaaaaat ggttagcaat
    51241 gtggatggat atcaatttga aaacgtgaaa gtaagcgcag aaaatgatgc tagactttat
    51301 cacgttttat ataatcggaa aaatcaggtt attgatggtt acgatcagtc tatgaattta
    51361 tcttcaagcg gattaacagt aaaagttgct gcaggagcag cgattattca aggtcgtatg
    51421 gtcgttgttc gacaagaaga aagtataaca gttccagcaa actcaagtgg ttatatagca
    51481 ttaacagtgg atttgacaca agaagttata cctggatcta ttcttccaga atcggaagaa
    51541 tatgaatgga ctaataatca agtcaagcta gaatttataa caaaagttat aaaaggtaat
    51601 ttaaacaatg gtgataaggt ctataattta ccactatgct cagttaattc tactggatca
    51661 actgtttcaa tctcaaagat atcggatagt tacgagctga ctctctctaa aggagaaatt
    51721 ttgtggaggg ggactgcgtt aatgcatgat actcaaactg tccaaccttc aaaaaaaatt
    51781 tggcagacag tttcaggttt tttgttagta tggctcccat acgaaaacgg gcaagcaatt
    51841 gaggatagat atgtaactac gcctttttat aaggagcgtg taacttttac taatgtttta
    51901 ggagaaattg tttcaggatt tgatgactat cacaaaaagt ggtttagtaa acgaataaac
    51961 tataattcga atactaatat atttacaggt gcagcaagca atgcaagtgg agataacgca
    52021 aatatggtgc ttaggtatat agtttctttt tagttaggag gtatggaaga gtggcaaatt
    52081 tagaaattaa attatctgca aataagagac agccttatct acgtcaccgt gttgttggta
    52141 gagttggtga tggggggctc acaacaatca atgtacaact tcttgaggaa gatgaaatta
    52201 caccttttgt aattaatcta aacggtactt tgaaatttgt gggcgaagtt tcaaacggta
    52261 actataccga gggagaacca gaaataatcg attcgactaa tgggttaatt agttacacgt
    52321 tcactaagtc aaatttcagc acgagtcatc aattcaaaca agcatatttt gaatatgtag
    52381 atcctaacgg caaaaaagta acttttcaga acttcatcat agacgtgtta gaacgagtgg
    52441 atattaattc ggagcaagcg aaatactata tttcttcatt ggaaaaatta cagagtgaaa
    52501 tgcaaaccac tttcaatcag ttcattagtg ataaacaggt ccaatacgat caaatctact
    52561 cgaaatataa cgaattagta agattgataa atgaatcaga taagcaagta aatgatcgta
    52621 ttgatcaaac caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca
    52681 gcatcgactt tgggggctat gattattcgg ggaatccgaa tttgttaagt aagctatcat
    52741 acgacttaat tgaaaatcaa aatacttcag ctggaacact ttctaagggt gaaaactcgt
    52801 ttaaatataa taagatatca gctgaagtgg aaggtggagt agagttatat tataaacgaa
    52861 gaggtatagc taactggtta ccctctaata aaacgcttgt aatgactgtt aagcttagag
    52921 ctggagcgga ctatagtcca gttgacggaa aacttatact gattagatat aggtatgttg
    52981 acagtggaac tggcaagatt gttttagact tacctattaa cagtaattcg ataactcagg
    53041 aatggaaaga gtttagtatt actggaacta ctccaacatt tagcccacaa gcataccatc
    53101 cttggataca atttagggct caagatgggg tacttgggga aatagaaatg agctatgaca
    53161 tcaaaatcga agaaggctct acagctacac catatcagcc taacttactt gatgactctt
    53221 actggctagg taaaacaccg ttgggtgaga atttgataac gaatgccaag ttcccaatta
    53281 taacacgaag caatcctatt tctggttttg atatttcaga agaattaata ataggtgaaa
    53341 cttatacggt atctttaaaa gggactaagc cagctactaa agaattccac ttatactacg
    53401 gagaagcaaa ttaccagcag ttgcagtatc aggcaacttt acttcctgtg gaaggattaa
    53461 cagatgtgtg gagtgccact tttacagcca gaaatactag tgaaacgaca aatttactta
    53521 gagtagcgtt atggcaaaaa cctaattctg caacatatga tactgttcaa attgactggc
    53581 tcaagattga aaaaggcgac acacgcacac caaatatcag tcagtttaaa tactttggtg
    53641 aaggcttgaa agacagtaac aatccgaacg actacagctg ggatgtcaca ccagagtata
    53701 ctgaaaaaag cttgaataat acggttagtc tgactgaacc gcaatccatc gaaggattga
    53761 aaaatttcga agatggtatt caatcaaaag gaaaatctgt attgacatca gacgacaaca
    53821 aatatgaagt cgtaacctta acagttacaa acgggaatac cggatcagca aagctttatc
    53881 gtgaaggaaa aaccgtcact atttattttt ttgcgttgaa tgggaaaagc agtggtggaa
    53941 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    54001 tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg
    54061 gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt
    54121 acacgattta gaaaggcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac
    54181 ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc
    54241 gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa
    54301 acatttggaa atatggccgt actggcggag agtatgcagg aaaagtattg gacgacatgc
    54361 ttgtatccgt tccttacaca gatcagccac cgctcgaagg ggttcgttca gatggcgaac
    54421 cgctaacgat tgctgatcag atgtttgatc ctaaattgaa ccaatggatt attttagcga
    54481 acgcgttaga tcacaacgat ttaaacaatc tcaaagcgat gtacgaggct cttgaacatg
    54541 aaaacggcaa cctaaaacag ctaaatgcca aactcatgct aagcgatgta gcgattaaac
    54601 aggaaaatac tgcattgaaa gaaaaagctg acagtttagc acaaatcaat tcaaaaatga
    54661 tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag
    54721 cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg
    54781 ggctgtttta ctgacgatca agttcgtcta ttcgttccac tatgtatcac agatgaagaa
    54841 gcagatagaa ttattagcaa agaagagagc gcatcttaag tgatgcgttt ttttgttgga
    54901 aagttggtgg aacatgaaag aagaagcgct ccaagacgtt gtggagagat tagtaagaat
    54961 tgaaacaaaa ttagacaact acgaatcact tagagaaaaa gctgatagtg caaaagattt
    55021 ggcagacaaa gcctactcag tagcgctaaa caacgcagaa gatatcaaag aaatgaaagc
    55081 caataataaa tggtcgtggg gttacatgat cggtttaggc attacgatca ttggctattt
    55141 cttgactaaa ttgtaaagga gttaagaaga aatgatttta cccgataagt attatcaagt
    55201 cattaaatgg acggttttaa cagtattacc agctgcatct gttttagtag ccacgttagg
    55261 aaaagcgtat ggatggaatg gaacagatat gacagtactc actatcaatg cagtagcaac
    55321 atttttaggc gttatcactg gtgtgtctgc atataactta aaaaaatagg aggaaaaaaa
    55381 tgaagaagaa aatcatttta tcattgagcc taataatggc tcttttttta ttgccaatta
    55441 acgggttcgc ctatacgatc aacaatgaat ttaatttagg tgtaaatgaa ggtagctcac
    55501 aagtagcaaa taaccaatac atcctattgc atgaaacagc aaacgaaact gcgacaggac
    55561 gaaatgaagc gcagtacatg aaacgttctt ggtacaatgc ctatacagcg tatattgttg
    55621 gcgatggtgg gatcgtttac caagttggac aacctggtta tgtacaatat ggcgctggtt
    55681 catatgctaa tgcaaatagt cctgtgcaaa tcgaactgca acatacgcac gataaagtaa
    55741 cgtttgaaaa gaactacaag gcatatgttg aattagcgag agattccgct aataaatacg
    55801 gtattccact tacattagac acaccgtata atcaaccagg aatcaaatca catttatggg
    55861 taactcaaaa catttggggc gatcacactg atccgtatgg atacttatca gaaatgggtg
    55921 taagtaagga aaaactggct tacgatttag ctcatggatt tacagacgaa aacccaacga
    55981 cttctgagaa caagcctgtc attgatccaa cccgagcagg tgcagcaaat ccaactttat
    56041 cagatggaac gaaccattct cacattgatc agtttggaga aatcgaaaat gcgaacttac
    56101 acgtcgctgg atggcacatc gctaactata aatacgagta tattttcatt atggactaca
    56161 atactgggaa agaattagct cgagtaagag ctgatggaat ttatagaccg gatgtaaatc
    56221 aagcttataa tacttctgga aacgttggct atcatgtatc ttttaacatg cgcaatttcc
    56281 ctaataagaa agtatacgtc atgatgcggg caacgaatga tccagaagga aacactaaag
    56341 gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat
    56401 agctcctcgt tgaggagcag tacatagaat tgaaaattaa cgttaaatca aaaaaatatt
    56461 tacaagatgt ttatttaagt agataataaa agaaaaaagc aggtgaaatt gtgacagcag
    56521 aaattggtat tatgaataaa agtggaatcg tgttagcttc agattcagca tctacgattg
    56581 gagatagcaa agtatataat actgcaaaaa aattatttac tttggattcc atgcattctg
    56641 taggtatcat gatatatggt aatgctgaat ttaatggtat tccttgggaa ataatcatta
    56701 ctcaatataa gaaaagcatt ggtagttctg tttttaatac tttggaagaa tacgcggata
    56761 attttataga atttgtcaaa acagcatctt ttattagaag tgaacagacc gagcaagaac
    56821 aaatgattgg ggtttttcaa aaaataattt caggtttatt tgagagtata gaagtagata
    56881 ttaacttttt aataagccaa ggtacacaaa tagacaaaga tgtcctagtg aagttattac
    56941 aaacaaaaat gaataccaat ttgtctcaac agtcccagac attcatttta gatattgaaa
    57001 aggcgttatt tcttagtaac tatggagaaa tattaaaaga tattcttaat agtatttcta
    57061 caatggaagg tgtttcagaa gctatatcag aggaaattca aagttacgtt tatgaaatta
    57121 taattagaga cgatgtttat tcttccccta caggaatagt tatagcagga tatggaagaa
    57181 tggatatttt ccctaaacta tattcttata atatgtttgg atttgttatg aatattctga
    57241 aatactcaga atatgaaagt gctcaaatag gaaatgacaa tggttcatta agatctacta
    57301 tattaccatt tgctcagtct gatgtggtaa atactgtagt tcaaggggta gatccacaaa
    57361 ttactaatta tctgtcgagt caagtcgata gttttgacga taacggaaaa aatacctata
    57421 ttaatattat aaagaatatt tcagagtttc aacaaaatca attcatattt ccgttactaa
    57481 acatgattgc tttactccca gttgaagaaa cagcaattat cgcagagact ttattaaatt
    57541 taaccagttt taaacgaaaa tatactactt ctgtagaaac agtaggtggc ccaattgatg
    57601 ttttagctat tacacctaat gatggcccta tttggattaa aagaaaacat tattttgata
    57661 ttgataataa tattggatac agattgcgaa aggagagagc gaatgattac aataattaaa
    57721 cctcaggaaa aatcccctgt tttgaaattt aatgagagca atctagccca taataaaatg
    57781 agtataactg atacagtatt tgatgtaatg gttgagcaac aaaaaaaaat taataataac
    57841 aatagacaaa gaaaagaaat taaccaatat taatgttttt tctgttgcgt tttttgcagt
    57901 cataatttaa aacctcgctc aatcttgggc gaggtctttt tttatttttc tgaaaagtta
    57961 aagtaaataa tagcataaaa taatatattt tacaaagaat aagtacaatc tagttttttg
    58021 ctattaaatg tgtaataata aatatgccat cacaacatga agaatgaaat ccattattat
    58081 ctagtctagt ccattctttt tgtttgcagt atttgtgatg gctttc
//
LOCUS       DASJPH010000006        52102 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJPH010000006 DASJPH010000000
VERSION     DASJPH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.006"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="U3995_000346"
     CDS             290..1135
                     /locus_tag="U3995_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1795698.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="U3995_000347"
     CDS             1416..2636
                     /locus_tag="U3995_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1795699.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="U3995_000348"
     CDS             2633..5353
                     /locus_tag="U3995_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1795700.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="U3995_000349"
     CDS             5337..6281
                     /locus_tag="U3995_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1795701.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="U3995_000350"
     CDS             complement(6350..7360)
                     /locus_tag="U3995_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1795702.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="U3995_000351"
     CDS             complement(7540..7884)
                     /locus_tag="U3995_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1795703.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="U3995_000352"
     CDS             complement(7877..8311)
                     /locus_tag="U3995_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1795704.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="U3995_000353"
     CDS             8449..9186
                     /locus_tag="U3995_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1795705.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="U3995_000354"
     CDS             9179..10390
                     /locus_tag="U3995_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795706.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="U3995_000355"
     CDS             10594..11376
                     /locus_tag="U3995_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1795707.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="U3995_000356"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="U3995_000356"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1795708.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="U3995_000357"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="U3995_000357"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1795709.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="U3995_000358"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="U3995_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1795710.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="U3995_000359"
     CDS             14978..16027
                     /locus_tag="U3995_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1795711.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="U3995_000360"
     CDS             16024..16302
                     /locus_tag="U3995_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1795712.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="U3995_000361"
     CDS             16277..16747
                     /locus_tag="U3995_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1795713.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="U3995_000362"
     CDS             16764..17066
                     /locus_tag="U3995_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795714.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="U3995_000363"
     CDS             17083..17454
                     /locus_tag="U3995_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1795715.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="U3995_000364"
     CDS             17426..17785
                     /locus_tag="U3995_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795716.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="U3995_000365"
     CDS             17957..18964
                     /locus_tag="U3995_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1795717.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="U3995_000366"
     CDS             18989..20173
                     /locus_tag="U3995_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1795718.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="U3995_000367"
     CDS             complement(20610..22193)
                     /locus_tag="U3995_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1795719.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="U3995_000368"
     CDS             complement(22771..22914)
                     /locus_tag="U3995_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1795720.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="U3995_000369"
     CDS             complement(23137..23616)
                     /locus_tag="U3995_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1795721.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="U3995_000370"
     CDS             complement(23689..23973)
                     /locus_tag="U3995_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795722.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="U3995_000371"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U3995_000371"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="U3995_000372"
     CDS             complement(24317..25603)
                     /locus_tag="U3995_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1795723.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="U3995_000373"
     CDS             25741..26412
                     /locus_tag="U3995_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1795724.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="U3995_000374"
     CDS             26378..26866
                     /locus_tag="U3995_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1795725.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="U3995_000375"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="U3995_000375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1795726.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="U3995_000376"
     CDS             27827..28591
                     /locus_tag="U3995_000376"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1795727.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="U3995_000377"
     CDS             28824..31037
                     /locus_tag="U3995_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1795728.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="U3995_000378"
     CDS             31048..31494
                     /locus_tag="U3995_000378"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1795729.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="U3995_000379"
     CDS             31665..32924
                     /locus_tag="U3995_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1795730.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="U3995_000380"
     CDS             complement(33013..33960)
                     /locus_tag="U3995_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1795731.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="U3995_000381"
     CDS             34354..35646
                     /locus_tag="U3995_000381"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1795732.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="U3995_000382"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="U3995_000382"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1795733.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="U3995_000383"
     CDS             37539..38414
                     /locus_tag="U3995_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1795734.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="U3995_000384"
     CDS             38680..40563
                     /locus_tag="U3995_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1795735.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="U3995_000385"
     CDS             complement(40638..40922)
                     /locus_tag="U3995_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1795736.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="U3995_000386"
     CDS             complement(40912..41646)
                     /locus_tag="U3995_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1795737.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="U3995_000387"
     CDS             41775..42398
                     /locus_tag="U3995_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1795738.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="U3995_000388"
     CDS             42590..43711
                     /locus_tag="U3995_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1795739.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="U3995_000389"
     CDS             43718..46846
                     /locus_tag="U3995_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1795740.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="U3995_000390"
     CDS             46865..50059
                     /locus_tag="U3995_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1795741.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="U3995_000391"
     CDS             complement(50205..50912)
                     /locus_tag="U3995_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1795742.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="U3995_000392"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="U3995_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1795743.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17095 a         9065 c        10782 g        15160 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaat taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatt ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ccagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag ac
//
LOCUS       DASJPH010000007        49464 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJPH010000007 DASJPH010000000
VERSION     DASJPH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.007"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..1974)
                     /locus_tag="U3995_000393"
     CDS             complement(247..1974)
                     /locus_tag="U3995_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1795744.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1967..3160)
                     /locus_tag="U3995_000394"
     CDS             complement(1967..3160)
                     /locus_tag="U3995_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1795745.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3347..3991)
                     /locus_tag="U3995_000395"
     CDS             complement(3347..3991)
                     /locus_tag="U3995_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1795746.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4085..4219)
                     /locus_tag="U3995_000396"
     CDS             complement(4085..4219)
                     /locus_tag="U3995_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1795747.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="U3995_000397"
     CDS             complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="U3995_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1795748.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6350..6823)
                     /locus_tag="U3995_000398"
     CDS             complement(6350..6823)
                     /locus_tag="U3995_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1795749.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7224..7448
                     /gene="nrdH"
                     /locus_tag="U3995_000399"
     CDS             7224..7448
                     /gene="nrdH"
                     /locus_tag="U3995_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1795750.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7607..9766
                     /gene="nrdE"
                     /locus_tag="U3995_000400"
     CDS             7607..9766
                     /gene="nrdE"
                     /locus_tag="U3995_000400"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1795751.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9816..10781
                     /gene="nrdF"
                     /locus_tag="U3995_000401"
     CDS             9816..10781
                     /gene="nrdF"
                     /locus_tag="U3995_000401"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1795752.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10870..12009)
                     /locus_tag="U3995_000402"
     CDS             complement(10870..12009)
                     /locus_tag="U3995_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1795753.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12318..13031)
                     /locus_tag="U3995_000403"
     CDS             complement(12318..13031)
                     /locus_tag="U3995_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1795754.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="U3995_000404"
     CDS             complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="U3995_000404"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1795755.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14220..15752
                     /locus_tag="U3995_000405"
     CDS             14220..15752
                     /locus_tag="U3995_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1795756.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15943..17145)
                     /locus_tag="U3995_000406"
     CDS             complement(15943..17145)
                     /locus_tag="U3995_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1795757.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17227..17916)
                     /locus_tag="U3995_000407"
     CDS             complement(17227..17916)
                     /locus_tag="U3995_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1795758.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18022..18390)
                     /gene="rplL"
                     /locus_tag="U3995_000408"
     CDS             complement(18022..18390)
                     /gene="rplL"
                     /locus_tag="U3995_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1795759.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18443..18946)
                     /locus_tag="U3995_000409"
     CDS             complement(18443..18946)
                     /locus_tag="U3995_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1795760.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19150..19277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19356..20045)
                     /gene="rplA"
                     /locus_tag="U3995_000410"
     CDS             complement(19356..20045)
                     /gene="rplA"
                     /locus_tag="U3995_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1795761.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20149..20571)
                     /gene="rplK"
                     /locus_tag="U3995_000411"
     CDS             complement(20149..20571)
                     /gene="rplK"
                     /locus_tag="U3995_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1795762.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20914..21582
                     /gene="sdaAB"
                     /locus_tag="U3995_000412"
     CDS             20914..21582
                     /gene="sdaAB"
                     /locus_tag="U3995_000412"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1795763.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21595..22467
                     /gene="sdaAA"
                     /locus_tag="U3995_000413"
     CDS             21595..22467
                     /gene="sdaAA"
                     /locus_tag="U3995_000413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1795764.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22558..23577)
                     /locus_tag="U3995_000414"
     CDS             complement(22558..23577)
                     /locus_tag="U3995_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1795765.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(23750..24655)
                     /locus_tag="U3995_000415"
     CDS             complement(23750..24655)
                     /locus_tag="U3995_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1795766.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24714..25259)
                     /gene="nusG"
                     /locus_tag="U3995_000416"
     CDS             complement(24714..25259)
                     /gene="nusG"
                     /locus_tag="U3995_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1795767.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25360..25530)
                     /gene="secE"
                     /locus_tag="U3995_000417"
     CDS             complement(25360..25530)
                     /gene="secE"
                     /locus_tag="U3995_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1795768.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25554..25706)
                     /gene="rpmG"
                     /locus_tag="U3995_000418"
     CDS             complement(25554..25706)
                     /gene="rpmG"
                     /locus_tag="U3995_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1795769.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25868..26512
                     /locus_tag="U3995_000419"
     CDS             25868..26512
                     /locus_tag="U3995_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1795770.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26568..27071)
                     /locus_tag="U3995_000420"
     CDS             complement(26568..27071)
                     /locus_tag="U3995_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1795771.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27084..27130)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27668..28633
                     /locus_tag="U3995_000421"
     CDS             27668..28633
                     /locus_tag="U3995_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1795772.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28732..29904)
                     /locus_tag="U3995_000422"
     CDS             complement(28732..29904)
                     /locus_tag="U3995_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795773.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29891..31036)
                     /locus_tag="U3995_000423"
     CDS             complement(29891..31036)
                     /locus_tag="U3995_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795774.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31050..31974)
                     /locus_tag="U3995_000424"
                     /pseudo
     CDS             complement(31050..31974)
                     /locus_tag="U3995_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32091..33170
                     /locus_tag="U3995_000425"
     CDS             32091..33170
                     /locus_tag="U3995_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1795775.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33202..33795
                     /locus_tag="U3995_000426"
     CDS             33202..33795
                     /locus_tag="U3995_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1795776.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33905..34132)
                     /locus_tag="U3995_000427"
     CDS             complement(33905..34132)
                     /locus_tag="U3995_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1795777.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34235..36823)
                     /locus_tag="U3995_000428"
     CDS             complement(34235..36823)
                     /locus_tag="U3995_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1795778.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36843..37502)
                     /locus_tag="U3995_000429"
     CDS             complement(36843..37502)
                     /locus_tag="U3995_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1795779.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37551..38054)
                     /gene="trmL"
                     /locus_tag="U3995_000430"
     CDS             complement(37551..38054)
                     /gene="trmL"
                     /locus_tag="U3995_000430"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1795780.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38275..39141)
                     /locus_tag="U3995_000431"
     CDS             complement(38275..39141)
                     /locus_tag="U3995_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1795781.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39250..39891
                     /locus_tag="U3995_000432"
     CDS             39250..39891
                     /locus_tag="U3995_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1795782.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39953..41314)
                     /gene="mgtE"
                     /locus_tag="U3995_000433"
     CDS             complement(39953..41314)
                     /gene="mgtE"
                     /locus_tag="U3995_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1795783.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41332..42231)
                     /locus_tag="U3995_000434"
     CDS             complement(41332..42231)
                     /locus_tag="U3995_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1795784.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42233..43030)
                     /locus_tag="U3995_000435"
     CDS             complement(42233..43030)
                     /locus_tag="U3995_000435"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1795785.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43008..43712)
                     /locus_tag="U3995_000436"
     CDS             complement(43008..43712)
                     /locus_tag="U3995_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1795786.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43843..44419
                     /locus_tag="U3995_000437"
                     /pseudo
     CDS             43843..44419
                     /locus_tag="U3995_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44537..45214
                     /locus_tag="U3995_000438"
     CDS             44537..45214
                     /locus_tag="U3995_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1795787.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45293..47101)
                     /gene="pepF"
                     /locus_tag="U3995_000439"
     CDS             complement(45293..47101)
                     /gene="pepF"
                     /locus_tag="U3995_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1795788.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47277..48275)
                     /locus_tag="U3995_000440"
     CDS             complement(47277..48275)
                     /locus_tag="U3995_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1795789.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48501..49154)
                     /locus_tag="U3995_000441"
     CDS             complement(48501..49154)
                     /locus_tag="U3995_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1795790.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14788 a        10395 c         8721 g        15560 t
ORIGIN      
        1 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
       61 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg
      121 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      181 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      241 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      301 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      361 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      421 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
      481 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
      541 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
      601 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
      661 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
      721 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
      781 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
      841 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
      901 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
      961 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1021 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1081 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1141 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1201 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1261 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1321 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1381 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1441 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     1501 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     1561 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     1621 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     1681 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     1741 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     1801 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     1861 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     1921 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     1981 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2041 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2101 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2161 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2221 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2281 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2341 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2401 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     2461 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     2521 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     2581 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     2641 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     2701 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     2761 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     2821 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     2881 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     2941 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3001 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3061 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3121 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3181 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3241 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3301 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3361 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3421 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     3481 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     3541 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     3601 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     3661 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     3721 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     3781 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     3841 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     3901 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     3961 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4021 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4081 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4141 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4201 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4261 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4321 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4381 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4441 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     4501 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     4561 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     4621 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     4681 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     4741 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     4801 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     4861 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     4921 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     4981 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5041 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5101 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5161 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5221 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5281 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5341 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5401 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     5461 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     5521 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     5581 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     5641 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     5701 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     5761 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     5821 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     5881 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     5941 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
     6001 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
     6061 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
     6121 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
     6181 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
     6241 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
     6301 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
     6361 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
     6421 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
     6481 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
     6541 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
     6601 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
     6661 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
     6721 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
     6781 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
     6841 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
     6901 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
     6961 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
     7021 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
     7081 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
     7141 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
     7201 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
     7261 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
     7321 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
     7381 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
     7441 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
     7501 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
     7561 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
     7621 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
     7681 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
     7741 aaatacgatg acttttctat ctattatgga aaaaatcaat tacttgatcg atcaagaata
     7801 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
     7861 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
     7921 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
     7981 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
     8041 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
     8101 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
     8161 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
     8221 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
     8281 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
     8341 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
     8401 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
     8461 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
     8521 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
     8581 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
     8641 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
     8701 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
     8761 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
     8821 ggatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
     8881 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
     8941 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
     9001 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
     9061 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
     9121 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
     9181 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
     9241 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
     9301 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
     9361 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
     9421 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
     9481 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaatggc
     9541 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
     9601 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
     9661 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
     9721 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
     9781 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
     9841 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
     9901 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
     9961 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    10021 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    10081 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    10141 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    10201 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    10261 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    10321 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    10381 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    10441 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    10501 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    10561 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    10621 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    10681 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    10741 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    10801 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    10861 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    10921 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    10981 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    11041 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    11101 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    11161 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    11221 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    11281 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    11341 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    11401 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    11461 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    11521 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    11581 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    11641 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    11701 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    11761 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    11821 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaac
    11881 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    11941 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    12001 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    12061 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    12121 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    12181 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    12241 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    12301 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    12361 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    12421 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    12481 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    12541 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    12601 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    12661 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    12721 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    12781 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    12841 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    12901 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    12961 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    13021 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    13081 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    13141 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    13201 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    13261 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    13321 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    13381 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    13441 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    13501 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    13561 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    13621 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    13681 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    13741 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    13801 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    13861 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    13921 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    13981 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    14041 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    14101 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    14161 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    14221 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    14281 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    14341 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    14401 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    14461 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    14521 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    14581 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    14641 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    14701 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    14761 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    14821 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    14881 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    14941 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    15001 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    15061 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    15121 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    15181 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    15241 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    15301 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    15361 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    15421 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    15481 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    15541 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    15601 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    15661 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    15721 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    15781 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    15841 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    15901 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    15961 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    16021 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    16081 accagccgca cctccaataa agtacataaa cataaataca gtattatttc ggttgcttgc
    16141 tgcttcacct agcccctgta ctcgtgtttg attggcgact tgaccaaatt gattcccgac
    16201 gtctaataaa ataatagcta aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc
    16261 aataaagctt attgtttgca taacaagtcc catatacacg atttttcttt cagaataaga
    16321 atctgccagt cgtcccacga taggtgcagc aactgctcca gacaaaccaa aaatcgctaa
    16381 tatccctgct tcgaatgttc cccaattgta ggcgggactg ctgatataaa aaatcaacgt
    16441 agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg cttctcttaa
    16501 tagacgttga gaagcaacta aatgcggcaa acttttaagt gaatcaaggt atgaaacagc
    16561 tgtactactt tgctggacag aacgcggcat tttccagtaa agcagaagtg taagaattcc
    16621 gactgcacat aaagcaaata aatagatcat ccgccaagaa aaaagactgg caacaatacc
    16681 agataccgtc cgagaaagca aaatccccac gagtaaaccg ctaagcaatg tacccatgac
    16741 cttccctctt gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac
    16801 gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa
    16861 aaaagcggcc aacagagaga cggcagataa cgctgccatt cgaatgatca attttggacg
    16921 atccaccacg tcccctaaag gaactaaaaa cagtaaccct aacgcatagc ctaattgggt
    16981 caacatcgtc aatatcccga ctgcacttgt gctaacagat aaactagctg ctacttttgt
    17041 tccaatcggc tggatgtaat acatattggc aacaacaacg ccacaagtga ttgataatag
    17101 taaaataagc gaagttgtta aaggtttttt ttcattactc ttcattgatt tcccccattt
    17161 cctatcttag tttacaactt atggaaaaga ttggaaatag aaaaatacaa ctattcgcca
    17221 cacttattat ttgtgctttt gttttccttg ttttttcttg cgatctttat aaatgaagac
    17281 aactaaccct actagcacag taacacttgc aaccaacgtg actgcagttt tgttatatcc
    17341 gtgaaacaga gcttctccac cacacgcata caacacagac gaagggatga ctccaatcac
    17401 gatcgacaat atgatttgct gtagaggcaa tttcatcgca tctgcagcaa aattgaccat
    17461 agaagatgga ataaccggta tcatatagcc taacattaca cctatttttg gatgtttcat
    17521 ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa attttaagcg
    17581 atgcataagg aaaattgcag tgatgttacc aagagcatta cccaaaatgt tgatgatcgc
    17641 acctgtcagt ggtccaaagc tcaaaccagc aatgactcca actactgaag taggaatacc
    17701 tggaacagca cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg
    17761 cgaacgaata tgtgcgagta gctctgcctt actcgctctc ggatttaaaa ataattggat
    17821 gtctgtctga tattctaaat acaaccgata gcccactacc agtatcagaa tgattcccaa
    17881 cgagatcaat atcaatcgga ttattttctt tttcatattt cctcctgaaa aaaatctata
    17941 ttcattaaat aatagtttaa cataaaaaca aaaaagcacc gaaatccaac ataggatctc
    18001 agtgctttta aatagtaaaa attatttaac agttacagat gcgccaactt cttcaagttt
    18061 agctttcaat tcttcagctt cttctttaga tacgccttct ttaactggtg caggagcgcc
    18121 atcaactaca gctttagctt ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat
    18181 aactttaact ttttggtctc cagctgcagt taattctaca gtgaattctg tttgttcttc
    18241 agcagcagca gcgccaccag cagcagctac agctacagga gcagcagcag atacgccaaa
    18301 ttcttcttca atagctttaa ctaggtcgtt aagttctagg atagttgctt cttttaactc
    18361 agcaacgatg ttttcaatgt tcaatgccat tataatttcc tccgtttaat tgttcttttt
    18421 tatttaatgc tttatgcggc aattaagctg catctccatc ttctttgtct gccactgctt
    18481 tgacagcgta agccacgttg cggactggcg cttgtagtac agatagcaac atagatagaa
    18541 gtccttcgcg atttggcaat tttgccaatg ctgtgatttc ttccaatgaa gagactttac
    18601 cttcgatgat accgcctttg atttctaatg cttttgcttc tttagcaaat tcgtccatga
    18661 ttttcgctgg tgcgactaca tcttcgttac tgaatgcaac agctgtaggt ccagtgaata
    18721 cttcgtccat gccttcaagg ccagcttttt tagctgcacg tgaaagaata gaatttttga
    18781 taactttcat ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa
    18841 ctgttaatcc gcggtagtca acgactacta ctgatgcagc tgctgtaaat ttttcgctta
    18901 cttcatcaac gattgctgct ttttttgcga taattgcttc actcattttt gagtttcacc
    18961 tcctgaattt tgatggaaga gttttttgca ctgaaaaggc aagtcacttt tatagttact
    19021 ttgctcaacc agtattattc atcacctatt cacttcgttc atagtgattc tactcgcttt
    19081 gctcgaaagg aaactccttt cgctttgctc aaccagtatt gttcatcatc tcttcacttc
    19141 gttcatagtg attcacaata aaaaactcta tgccaccgca gacatagagg gactattgaa
    19201 tatattcaat aaacgtcctc ggtaggaaat taaggcttga tgccacctac tgtcttcggt
    19261 acaagttaat tattaacctc aactagctta gcatgctaaa ctagttgagt caagaattat
    19321 ttttcagttg gatcaatcaa cattctaata tttaattaga aagaagcttg gtcaacatgg
    19381 ataccaggtc caaatgttgt tgtgatcgtt acatttttca tgtattgacc tttagctgct
    19441 gatggtttag cttttagcaa tacatcatgg attgctgcaa agttttcaac taatttttca
    19501 tcgtcaaatg aaacttttcc gattggtaca tggatatttc cagctttgtc cacacggtaa
    19561 gttactttac cagctttcac ttcgtttact gcttttgtta cgtccattgt aactgtacca
    19621 gttttagggt ttggcattaa gccttttggt cctaatactc gtcctagacg acctacagta
    19681 gccatcatat caggtgttgc aacaactaca tcaaagtcaa accatccgtt ttggattttt
    19741 tgaaccatgt cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc ttttgctttg
    19801 tctcctttag caaaaactaa aacagtttgt gttttaccag ttccgtttgg taatacaaca
    19861 gcaccacgga tttgttggtc tgctttttta gggtctacgt tcaaacggta tgcaacttca
    19921 actgttgcat caaattttgc aatgtttgtt tcttttgcta aagctactgc ttcggcaaca
    19981 ggataaactt tacttgaatc aacttttttt aatgcatctt gcattttttt gctcttttta
    20041 gccattttgg ttcctccttg attgtggtta taacggttgt acctcccacg tgttctatat
    20101 ctttcaatat agagccaact gagaagctgc tgtcttaggg tgtatagatt attctacagt
    20161 gatccccatg cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa cgttagctgc
    20221 gtttaggtct tccattttta attcagcgat ttctcttact tgatcgctag aaactttcgc
    20281 aactttattt ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt ttttcaatag
    20341 aacagcagct ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat
    20401 aacaaccgga atgatcaaac ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa
    20461 tcccatgata ttgatacccg cttgacctag tgcaggacct actggtggag ctggagttgc
    20521 tttacctgca ggaatttgca atttaacgat tttttctact ttctttgcca cgagacatac
    20581 ctccttaagt ccgtgatgtg gttaattgga gatgcatatt tctcctccca cgttttatct
    20641 gatctgaaaa aatccagaca aataatgtgc aaaacaatgc acactggtac agtatagcaa
    20701 atttatttgt tttttcaaga tttttttctg ctatttttac attttgcttg aataaacgct
    20761 aaatttattc taaaaaaaca acaatttttc gttctcattt cttcttttat gttacacttg
    20821 cgctagcatg gttcaacaga tgaaagcaaa caataacacg gttttttaga cccttgtaca
    20881 aagattccta cataacaaaa tgaggtggag aaaatgaaag atttaaggta tagaagtgtc
    20941 tttgatatta ttggacctgt gatgattggt ccaagtagct ctcacactgc cggagctgct
    21001 agaatcggga aaatcatacg tagtatattt ggcgaacagc ctgattctgt agatatttat
    21061 ctatatgaat catttgcaaa aacctatcgg gggcacggaa cagatattgc attagttggc
    21121 ggcctgttag ggatggaacc cgatgatgaa cgtttagctg attctttgaa attggcatat
    21181 gaagccggta tggaagtctt gtttgttcct aagagtgaaa aagccgaaca tccaaactct
    21241 gtaaagatgc ttctaaaaaa aggaacacga aagctttcag tcactggcat ttcgatcggc
    21301 ggtggcaata tccagatttc tgaattgaat ggattcaaaa tttctttaag catgggtact
    21361 cctaccttga tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt gactagtata
    21421 ctttctgaga ccaatacgaa tatcggcact atgacagtga cacgggaatc aaaaggtgaa
    21481 aatgccatta tgataattga agtagatgat cctcaagtag aagaaacggt aaaaagactc
    21541 aaacagctgc caaatatcga cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt
    21601 ttatcgattg aagaattagt agatcaagca aaagcttatc caagtgtagc agaattgatg
    21661 attgcagttg aaatggaaat gagtggccgt tcccgtagtc acatcatcga gatcatggaa
    21721 aaaaacctct cagtaatgga acaatcaatc aacgagggaa aagaaggtgt tacctccgtc
    21781 acaggaatta ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt
    21841 ttaagtggag aaacaatctt aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat
    21901 gcaaaaatgg ggttgatttg tgctacaccg actgccggaa gcgcgggtgt tgttcctggt
    21961 gtgctgatgg ctgtccgaga tcggcttgat ttgacacatg aacagcaatt ggaattctta
    22021 tttaccgctg gcgctttcgg gttagtcatc gcaaacaacg cttctatcag tggtgcagaa
    22081 gggggatgtc aagcagaagt tggttctgca agtgcaatgg cagcagcagc tttggtttgt
    22141 gcaaaaaatg ggactcctca tcaagcagca caagcagtag ccattacatt gaaaaatatg
    22201 atgggactga tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat
    22261 gctttaggct cttctcaagc ttttatctct gctgatatgg cattagcggg tatcgaaagt
    22321 gtcattccgc cggatgaagt cgttgctgca atgtaccaag tcggacgtca aatgccgtcg
    22381 attttcaaag aaacagcaga aggtggctta gctgttacgc caactgctaa acgacttgcg
    22441 gaagagattt taaataagag ggcgtgataa agctccttaa tttcgagtat taagagatta
    22501 gttatcttca taacaaaaaa cgaagagagg actaattgcc cttctcttcg ttttttatta
    22561 atccatcaat ttcgtatatt ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt
    22621 gatcgacaat tctttgttca ggtgttcaaa aacagatgtt actccttctg ccccacctag
    22681 attcaaccca taaatcactg gtcggccaat cgcaactaag tcagcaccac ttgccagagc
    22741 tttgaagaca tgctctcctc gacggacacc agagtcgaaa atcactggta cacgtttgtt
    22801 gactacttcg gcaatttttg gcaatacttc aaaggacgca ggacctccat ctagttgacg
    22861 tccaccatga ttggataccc agataccatc tgcccctgca gagatagcga ttactgcatc
    22921 ttctggcgat tgtattcctt ttacgataac tggaagatgg gtgatttctt tgattgtttt
    22981 gatgtcatct ggtgtcaagc cttgttttgc agctgcataa atttctgcga ttccttttcc
    23041 ttcgccatta ccacttgcac tttgttcgct atacgcagct aagttaggca ttggtaatgg
    23101 aaattggaat tgattgatga cgtcttcttc acggtaacca ccgagcgttg aatctgccgt
    23161 taaaatgata gctttagccc ctgcttctac tgctttgttc aaaatgaact cattgaatct
    23221 gtcgtcttta ctcatatata attggaaaaa ctgcggtgcg cctggcgctg catcggctgc
    23281 gtctttgatt gtcgtatttg cataagtgct gatggaaaag attgatccag cagcagctac
    23341 tcctttagct gtatcagctt cccctttttc atgtgccaat ccttgggccg ctgatggcgc
    23401 ttggataatc ggtgtatcta attcaattcc gaaaacagat gtatgcaaat cggctgagtc
    23461 gattccgcgt aatatccgtg gcatgatttt ctttgtatta aaagaagtcg tattttcttt
    23521 catcgtccac tcatcttcag aaccaccgcg aatatagcca aaagctcctg cttccatgcg
    23581 ctcttttaca cgaccttcta gttaggcaat gtttacaata tcgattggat gttcttcatt
    23641 acttgtatga taagtcatag tagatccttc tttcatcttc ttgattacat ataaatcata
    23701 ctaaacttac aaaaagtaag caaacattta ctctcagata cttttatcgc tattctttcc
    23761 aaataaatcg acttacaagc tgatctactt cttggttttc atgaagcata ctatgagagg
    23821 cattttcacc tgtcactact ttttcctcta tttcatttcc gcgttgcttc aacaacgcat
    23881 agaccgctaa tgcactgttt aacggtactg ttccatcact caagtctgtt tcatctagtt
    23941 gaccagcaat caagaaaaat cgagtggtta taggaatatt accgatcatt tgctggtaat
    24001 cttgatagcg gttagaaacg acagcaggcc cctttgctaa ctcatctgtc agagactgcg
    24061 cactatcatc gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg
    24121 gcaatgacgt atcctgtcca tacgttccta aatagcgtag tgcactaact cctcccatgg
    24181 aatgccccac tatgttgact ttatctatgc cgtagttttc tttcagatac aaaagacaag
    24241 ttttgatcca ttctgcttga ttccactcgt tatttttatt atcttcaaac aatacttgga
    24301 ccatcggatt gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc
    24361 ctactgttaa aaccaattct tttactgctt gctgattttt ttccaaacgc cgcaacatcc
    24421 ctttaaagga attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt
    24481 tttctatcac tttactggat tctgccgttt tttctttatc agaaacttca gtagtattta
    24541 tcttcgtcgt ctggcaacca cttaacgcaa acaaactgat aaggactaaa gctgctgcag
    24601 ttgcacgttt gcataaattt ttgattggtt ctgtcataca cctcttcctt tccatataga
    24661 aaaaagaaag acttgaaagg ccaatcatat gatcgacatt tcaagtctct ttattaatcg
    24721 attttatcta cttgatcgaa atcaagttct gtactggttt cacgtccaaa catatcgatg
    24781 ttgactttca atttttcttt ttctccgtca acttctgtta cttgtccttc taatccagca
    24841 aatgcgcctt cgatgatttt cacgacttcg ccaacttcta cttctagatc attttgtcgc
    24901 aggctcatac cgatagaacg caagatatga ttgacttctt ctggaagtaa tggtgctggt
    24961 ttgcttccgg ctccgtgtga accaacgaat cctgtcacac caggtgtgtt gcggacaaca
    25021 taccatgaag cgtctgtcat gatcatttca acaagtacgt atcctgggaa tgttttatga
    25081 acgatttctt tttctttccc atttttgact tcagtttctt tttcttcagg aacaacgaca
    25141 cggaaaataa agtcggccat tttcatactt tgcgcacgag attcgatatt tgcttttact
    25201 ttgttttcat atcctgaata tgtatgcaag acataccagt ttttttcaaa agattccatt
    25261 gaaggctcct catttcattg taaaataaaa aaacctcagt acacccgaag tttttctttc
    25321 gcttcattat agcactaaaa gtacgttgtg gctagcgagt tattttaaaa tccagccaaa
    25381 taagctttgg atggctgtat ccattacgta aaacatcaat ccgaaaataa tagaagtttc
    25441 gatgactact aacgtatctt tacggagttg ttttctgctt ggccaagtta cttgtttgat
    25501 ttcatctttt acgcttttta gaaatttcat gagacaacct cccttgctac tgtttatttc
    25561 gtttctttat gtaatgtata ttgattacaa tatttacaaa acttattgat ttccaaacgt
    25621 tcgccgcttt ttccttcact gactgatttt gtgtagttgc gagacccaca aacggaacaa
    25681 gcgagtgctg cttttttctt tgccatttac ttctgccccc ttgctgagaa tcagacttat
    25741 acttcataac agtatcacgt aactttttac ttgtcaattt gtgaaataaa ctatctattg
    25801 ttttggataa aattatgata tgattaattt gaaattaagc taaaatgaga caattggagg
    25861 tatagcgatg ctattaaaaa cacttgtata taagaaaaaa gatctgacaa ccgttacaga
    25921 gactagtaca ttagaagaag cattaaaaat tttagaaact tccggatacc gttgtgtgcc
    25981 gatcttagat tcttcaggta atattttccg tggaaacatt tacaaaatgc atatctatcg
    26041 tcataaagca aatggcggtg atatgacgct tcctgtcact catcttttga aaaacgctac
    26101 aaagttcatt tacctggata cttctttctt caaagtattc tttacgatca aagagcttcc
    26161 ttatattgct gtccttgaca gtgaaaatca tttttatggt attttgacac atagtacgtt
    26221 gcttaatatg ctctctcaat cttggagcgt agaacaagga agctatgtac tgacaattgc
    26281 ttccacagga aagcaaggag atttggctgc tattgcaaaa atcatttcaa aacacagcag
    26341 cattgccagc tgtattactc ttgatatcga aaaagacgaa tacattcgac gtacattgat
    26401 cacgctaccg gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa
    26461 aaagaattat aaagtggtag aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag
    26521 cgaaaaaaga aaacagctaa tctgctttct tttttcgctt tttataatta gacacgcttt
    26581 tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg ctgaaacagc catgacgata
    26641 aactcactta atgcaagcgt gccataggtt gtccaaaatg cttggccacc tgttggaact
    26701 aacatataag caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc
    26761 atctttacat ttttcacttt gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt
    26821 tggccgccac caaaaaccac atccatgata ccaaaaccaa agaaggcatt atagacgatg
    26881 accccgccta aaactcccca aagcaattta cggttaaaga caactaaatg attcaaactt
    26941 tctgaaatac gaaactggat cggccctgag gatacaggcg caactaaaat agttaacgct
    27001 agataaagag ccatgatgat gccatttaca acgattacgg ataaccgttg tctgcttttc
    27061 gttttttgca ttttctctcc tcttttctcc tagttttttt aacgcgggat ggttgatgaa
    27121 ccgcggttat gtgaaacaaa caataattat aacagacttt tcatttattt caatccgtct
    27181 tatctgcttt taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga
    27241 tatggtataa agtaagtgta aggggtcata agaattccca gtaaagcaga cgaagtagga
    27301 tctgcaagaa cagaattgtt gtgaaactta aataaataaa aaaggatgtg ggaatttgag
    27361 caacctctta ctagagaacg gtctagcaag cagctgaaga tctaagtgga catgccgaac
    27421 cttggaactt ctccttacgt aagtaatgga tcatgcgctt gctaggcttt tttgtgtttt
    27481 tcggtcttcg gacctttttc attttcctag acttctgcta taataaccaa gaattaattt
    27541 tgtaacagat ttttttgaaa aaacaagttt ctttcattca aaaaatagaa aaagcttgct
    27601 aattattggt taaccagcaa acaggagtat taaaatatct tgttttattg aatagcagga
    27661 ggaaatcatg aaattccatt atcatatatt gatcaatcca tcggcaggca gcggaaatgg
    27721 ctataaagta gcagaaagaa ttttaccggt attaaaaaat aagcatattg actatacact
    27781 tcattatagt gaatacaaag gacacgatgc tgagattgct gaaactttgg caaaagaaac
    27841 attgattcct tggatagaag aagaacagaa aacaatcgat actttccccc ttttgatcat
    27901 cgtaggaggt gacggcacct tacatcaggt acttgatact ttctatcaga tggaagtaga
    27961 attcccagtt gcttatattc cagcaggttc tggagatgat tttgctcgag gggcaggcct
    28021 tccaaaaaat cccaaaaaag gacttcagct gattttagca gctcaatcac cagaaaaagt
    28081 ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt
    28141 cggtatagga ttagatgcag ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg
    28201 attgaacaaa tacaacttag gatcattttc gtatttattc tctattctcc gtgctttatt
    28261 cactcaaaaa ggatttccaa ttctggtaga tgcaggagga aaacaattaa gtttttcaaa
    28321 cgcattttta tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg cgattgttcc
    28381 aactgctgat gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt
    28441 taaaatcttg tggctcattc ttttacttat acagaaaaaa caattgaaat cgaaatattt
    28501 ccatcactac acaacaaaca gattacgtat cgtttcaaca acaccgcaac atggtcaaga
    28561 agatggagaa gaaatggaaa aacgaccttt tgatatcaca ataaccacaa ctaaacagtt
    28621 gttttggtgt taataaaaga ggttttgtca gaagctacat ctggaacacc gtttattcgg
    28681 aaaaaaggac tgtgacacga cttttgtcac agtccttttt ttactatgca gttaattctt
    28741 tgcccaattg ggttgccatc gatagtttct ttctttaccc aaaataaatt ggatcattga
    28801 aaacaccgca gcaaaagcaa ctaagacaca accattgaac caaaaagatt ctgcaaatgt
    28861 ttgattgtaa ctgagtgttt cactgatcaa gttattattt tgaacaaacc aatatgttgg
    28921 tgtgaatgcc gctattttgc taaccatttc tggcaagtat tctgctggta caaacacccc
    28981 accgataaag caactgcctg tgataaaaat atctttgatc ccaccgctgg tttctgagtt
    29041 ttttaccaat gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt
    29101 atttaaaata tgataccata ccgttatatc ccacgaggtg tggatcacta acagaaccat
    29161 tagaagtaag aagccactga acaacagcag cgagtaacta atcaaactaa aggttacttt
    29221 gcgattcaag tttcgtttgg taatgggcgc actctggttt cgtttactga tctctttgcg
    29281 attaaatgct agattgatga cgctgaaacc gctaaaaatg gatagaaaca acccataaga
    29341 caagaaatta aaggttgtgc ccgtcaacag ctttttcatc cgctgactat atcccgcact
    29401 tagctcaact tctccagtaa ccgagagtgc catttgtgtc tgttccatag ctgcatcgat
    29461 tgttttatct tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt
    29521 aacaactgtt ttagaaaagg catctgggcg actctgtatc ttgatcgtcg gcatctttcc
    29581 tacttcgact tgttcggtaa agtctgcagg cagatgtaag ataatgttta cacgattaaa
    29641 ataaagagca tcatcgattt ctttttgtga ggttccatcc aatgtaacga ctgtttcctt
    29701 gtcattcaaa tatttacgaa ggccatcgac tacaggcgac tcttcttcac taatcaaggc
    29761 gattttcgca tcttgcagag aggtattttc aacatcaata ttttgcgcaa aaaagaatgt
    29821 ggcaacaaca gtgataatta cacctatcag taaattcgat ttatttgctt taagcaatgt
    29881 ccaaatcgtt ttataaagcg tcataacgca cccctctttc catccaagca tttcctaacg
    29941 caaaaaggaa aataaatccg atcaaccata gaagattggt ataaaaacta gccaaatcag
    30001 tgtagaaata aagtttatac aaactttcgc tgatcaagtt gacgagattt aattgaccaa
    30061 caatcggaat atgaagatca atccaatact tcacttgttc cgaccccatc ataccagcaa
    30121 aaaagctcat cgcaatcgtc actgaaatca agatgctatc cttttgcacc agattcattt
    30181 ttggtatcaa attaccaacc aatgtaccta acaaaatcgc acacaacgac cccagagcgg
    30241 taacaatcat caaccattgc caacgttcac caaagtctac ttgatagata aaacgaaaga
    30301 cagctaacat aatataaatc tctgtcaaaa caatcgtaaa gcttgctgcc aaattagcga
    30361 ttgaaactaa cgattgcttt ctaggaatca aggacaagcg aataccattt gacgattgat
    30421 ttgcttgctg atcctgtaca ttgcggaccc cccacatcgt cccgtagaga atcgccattc
    30481 caatcagagt gaagaaataa aaacttttga aactcatatt acgacctgca tgaacttctt
    30541 gaaaagatac ttcttgttca aaaacagtca tgatgggcgc gagatcaacg ttctccactt
    30601 ctgccacttt actcgaaatt ataccaatac gctgtaaaaa ttgatccatg acgtttttca
    30661 tcaacgtctg actcattcct gcttgaccca catggagcga aacagatgaa ccatcaatga
    30721 gataaaaacc agccaaatca tctgcattta gcttttcatt cgcttctact tccgatagtt
    30781 cctgtacttc aaataatggt ttatcattga cttcgacttg ttctaagact gtggtaaaat
    30841 tttccgaaag cgctgtttga tccgctttga ttacaattcc agtattaatc gtttcgagat
    30901 tgccaccttg atcaatctcg ccaaaggccg ccataaacat caatcctaaa agaattggaa
    30961 aggccaatgt ccaaaacaac atggatttat ttcgcagcag tatttttgtg cgatatttat
    31021 aaaaatgcca aaacattatt tcccctcctc tagtctctca aatccttgcc agtaatctct
    31081 aaaaagacat cattcaagga cgcttctttg gttgctattt ttccgtaact gatcttttct
    31141 tcttgtaaga ttgtaagaat cggcaacaat actgtattta gttcagtagt tgttacttgg
    31201 agctgacctt ttttataagc gattccatca gaagctattg ttcgctgaca acacgttttc
    31261 tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga
    31321 ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag
    31381 aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag
    31441 ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc
    31501 atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt
    31561 caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg
    31621 aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa
    31681 ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac
    31741 cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat
    31801 ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg atttcccact
    31861 gccgtttggt cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt
    31921 caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa
    31981 aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt
    32041 gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat
    32101 ctcggctact caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc
    32161 aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca
    32221 gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg
    32281 cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag
    32341 aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc
    32401 atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa
    32461 aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt
    32521 gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac
    32581 aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg
    32641 tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag
    32701 aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc
    32761 ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag
    32821 agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat
    32881 tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta
    32941 tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc
    33001 gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt
    33061 tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa
    33121 accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt
    33181 cattattgag atgatccatt tgtgacgaaa tcgttacaaa ccattttaga aagcacgaaa
    33241 gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt atacgaaacc
    33301 catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg catcgatgct
    33361 gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac gacattcaaa
    33421 gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt aatcaaacag
    33481 aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc cgtatttggt
    33541 aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat tgatagtcat
    33601 gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc tgccgggaaa
    33661 aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg caattacatc
    33721 agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat ctatttttat
    33781 cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc cttcgcatca
    33841 ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct cacgattgaa
    33901 agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg ttaggcggaa
    33961 aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg tctcgttcta
    34021 catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt ttaaagtcat
    34081 tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc ataaagtaac
    34141 gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa aacgctaacc
    34201 aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc ggtataaaat
    34261 cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta tcgatcactt
    34321 ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc ttttcttctc
    34381 caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt ttcgtttcat
    34441 tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc tcttttgtat
    34501 agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct gctagtttgg
    34561 tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac gtagtcatcc
    34621 ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct aacaaaatca
    34681 acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc atccgctgat
    34741 attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat ttatggattg
    34801 agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg acctcatttg
    34861 catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc aagatgatac
    34921 ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg actaattgga
    34981 ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt tttccaggtt
    35041 tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct gcaggtcccc
    35101 gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt ttcgcttttt
    35161 ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa taggaacgat
    35221 ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca tgggcaagag
    35281 gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt ttgggaattt
    35341 gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga agctgatctt
    35401 tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat gtgttagcca
    35461 gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct aattctttcg
    35521 tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg atcgtactgg
    35581 caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt gctgcaagta
    35641 gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg ttcaactctg
    35701 caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt cctcctgtta
    35761 aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa ggtccatact
    35821 gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt tcaggataat
    35881 ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc cattcagaaa
    35941 agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct tcttcaacaa
    36001 gttggataat tacagtagca acctgatcag gctggatttc aattgggcga cttgtttcta
    36061 aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct cctgtttgaa
    36121 ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt gaatctgctg
    36181 caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct tctatgtctt
    36241 cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta taggcctggt
    36301 agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg atcacttgat
    36361 ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc ttcgtcaaac
    36421 cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca tcttctccca
    36481 gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt tcgctggaaa
    36541 gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt tcgagtttaa
    36601 gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa gtctcatttt
    36661 cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa tcagctgtgg
    36721 tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga aaaaaaatcg
    36781 cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc cgcctccttc
    36841 ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg gttccatact
    36901 ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt caaactacta
    36961 ttgaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat ccattgaatg
    37021 gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt atgttgggca
    37081 accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt ttctccgtga
    37141 aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt atccggatat
    37201 ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag ttctcttagc
    37261 ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc ttctgctgta
    37321 tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata ttttaccttt
    37381 ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc gccattgctc
    37441 ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa atatagttcc
    37501 atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt ctaatccagc
    37561 ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg gcgaagtgct
    37621 tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg ctcatcattc
    37681 atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg caaaccggtt
    37741 gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc gtcactatat
    37801 actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa tgttaaaaaa
    37861 gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa ccctgcccgt
    37921 tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag cggagtattc
    37981 gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg ttcaaataaa
    38041 acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat tttctgacaa
    38101 tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg agtcgttacg
    38161 ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa accaatgaaa
    38221 aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta ctcattaaaa
    38281 actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa ttgtaatctt
    38341 cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc attctaaagg
    38401 tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat aggttacacg
    38461 ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt cattggaata
    38521 actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta aataattttc
    38581 ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga attcttggta
    38641 atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa acggcaaatt
    38701 ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg cgtagactcc
    38761 ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg aaagttcatt
    38821 caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag gtaaatgctg
    38881 cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac gccttggaac
    38941 gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa gtgttccttt
    39001 tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag acttttttac
    39061 ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa actggcgtcc
    39121 tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac cgttagatgc
    39181 tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa agataaagga
    39241 gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc tctagcaatt
    39301 gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg cacaaccccg
    39361 agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat ccccgtaatg
    39421 acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt gaaaatcatg
    39481 catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt tggttgtctt
    39541 actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac agcagaaggg
    39601 ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca attcgaagca
    39661 atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg tcctgctgac
    39721 aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac agatggccgc
    39781 ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt agctaaagaa
    39841 ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata atcttataaa
    39901 caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt ttttacataa
    39961 aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc aaatcgctca
    40021 aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga tccattagca
    40081 tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata gccatcccta
    40141 tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa atcgtgatcc
    40201 ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt cgaacaaatc
    40261 catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc gactgggttc
    40321 cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata ctcgcttcac
    40381 ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa aacaacagag
    40441 tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta atttcttcca
    40501 cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata acgtcgatga
    40561 tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca ggaagagcta
    40621 agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct acatgaactg
    40681 aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc acgattaggt
    40741 cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag atgtaataga
    40801 ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga acagtttgat
    40861 tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca tcctcgtaat
    40921 ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa tatttagcaa
    40981 tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac atctcagaaa
    41041 gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata tgttcgttgt
    41101 cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag gaatagatca
    41161 gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag atatgcatct
    41221 ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga gtcaactggg
    41281 caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc cttattcttc
    41341 aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact ccagcaactc
    41401 ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc tttttattcc
    41461 cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa ttgcggaaaa
    41521 atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta acgcgatttc
    41581 tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat cagagaccat
    41641 tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc catgtgccga
    41701 taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact tcttttcacg
    41761 aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca tcaaaccaga
    41821 tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac cttgtttttt
    41881 ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg caggaataga
    41941 cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca cgatatcgat
    42001 cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca caatggttac
    42061 cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa ctttgatttg
    42121 accgccttga aattttattt tcgctaacag accctttgag atcccttttt cttttaaaaa
    42181 atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca tatcaatcct
    42241 cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa gaagcaaaat
    42301 ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt gcgacattca
    42361 attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct agccattcat
    42421 catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc agtgaagcaa
    42481 tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg acacttttat
    42541 tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta aaactttcaa
    42601 acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt ttaatcgttg
    42661 attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg atccgaacat
    42721 ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct actagttctt
    42781 ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc acgcccaaga
    42841 aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat gtgccgtctc
    42901 cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct gcttgttcta
    42961 aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta tgaacgatcg
    43021 ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt tcgatagtag
    43081 acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg gatttcttca
    43141 cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg ctgaagtcgt
    43201 tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg ctcaatcgtt
    43261 gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt cttctcaccg
    43321 ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc acttgctttt
    43381 ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt acggatgatc
    43441 tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc ggcaatatca
    43501 gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt tgttacaata
    43561 ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg attttgttca
    43621 cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt ttgttcatat
    43681 ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc attaacattc
    43741 attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg cattgaccaa
    43801 aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca aatgcttgaa
    43861 atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc tcactatcaa
    43921 ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt tgatgaacag
    43981 ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc tgaatataca
    44041 ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat aacagaagaa
    44101 gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt tcaaaaactt
    44161 tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac aacaaaacgg
    44221 gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg agacaatcta
    44281 cacgattttg aactggaact agaggtaagg ggatgcatct gtgggaaaaa ttgcttttat
    44341 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    44401 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    44461 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    44521 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    44581 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    44641 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    44701 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    44761 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    44821 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    44881 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    44941 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    45001 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    45061 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    45121 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    45181 aagaactagc catttacact tgctttctaa ttaaaaaaat acgtcgactc ccttacaaga
    45241 gagccgacgt atttttttcg tttctattcg agaaaatcaa aaatagaaag ccttataaac
    45301 gatcgatcaa ttcttctaat tcatttagac gttgttcaaa catcgacatt gcatcttcaa
    45361 tatatgcagc ctgtgtcata tcaactcctg cttttttcat tacttccaca ggataatcgc
    45421 tgttacctgc ttttaggtaa gccaagtagt tctctagtgc ctctggttcc tggtttagta
    45481 ttttcttcgc cagtgctgaa gcagcagaaa agccagtcga gtattggaaa acatagtaat
    45541 tataataaaa atgcggtatc cgtgaccatt cgaatttaat ttctgggtct tcttcgactg
    45601 ctggaccata atattttgca ttcaatttac cataactatc acttaggtat tcactagtca
    45661 atggcacacc tttttcatct tccgtatgca tgaaatgctc gaattcagcg aattgtgtct
    45721 ggcggaaaac tgttccttta aacccatcca agtaatggtt gagtacataa gcccgtacac
    45781 gagggtcttt ttctgtttcc aataaatact ccgttaggat attttcattc gttgtcgaag
    45841 caatttctgc taaaaagatg gagtagtcgc cgtacacata aggctggttc gaacgagtga
    45901 aataactatg aacactatgt cccatttcgt ggacaagcgt aaatagctga tccagcgtat
    45961 catgccaatt caataaaata tatggatttg tgtcatagct tcccgaagaa taagcaccgc
    46021 ttcgtttccc tttattttcg acaacatcga tccaacgttc agagaatgct ttttctacga
    46081 tggccatgta ttcttcaccc attggtttca gtgcttctaa agctttttct tttgcttctt
    46141 cgtacgtaaa ggtaattgga gcttccccta agaccggtgt ataaagatca tacatgtgca
    46201 gtttttccac ttctaataaa cgtttccgta attccatgta tcgatgtaac aaaggcaaat
    46261 gcttgtttac cacgtctacc aaagtatcgt atacactttc aggaatatga ttattgctca
    46321 aagacgcttc tctggcagag ctgtaattac ggactttcgc tttaaaatta tgtcctttta
    46381 tatgtgtgcc taaagtagaa gcaaatgtat ttctaaattg ttcgtaaaca ctgtacaatc
    46441 ccttaaatgc tgcttcgcgc acccttcgat ccgtgctttc tagcaactga ccatacacgc
    46501 catgagataa ttggactatt tcaccatttt ctccttcaat cgttggaaaa actagatctg
    46561 cattattcaa aacagcaaat gtatcacttg aagcatcaaa gatttcacct gctccagcaa
    46621 gaagagattc ctgctcagca gataagacat gggctcgatt atctactatt tgctggatat
    46681 aatggcgata gacttccaat tttggttctt ctttgaaata ttgccaaatc tggtcatctg
    46741 acaattgcaa tatttctggt tcaaaccagg aaacggcttc actaactttc gaaaacagac
    46801 tgcttgctct tgcataaagc gcttggtaat ctgtatttcc agtgtcttga tcgtttttaa
    46861 ggtgcgcata tacataaatg acttcagttt ggcgataaac cctcaatacg aattcaatcg
    46921 catttaaaaa ttgagaagcg ccttgatcaa gtgttccttt gtgtttttca gattgtttta
    46981 actcttctga taattccaaa tatttctcat caaactcttg gtcgcttgaa aagatcttgg
    47041 tcaagtccca agttaaattt tcaggcaatt cttctcgttt tggcaactgc tttacttcca
    47101 tgaaatccac tccttttctt cccttagctc tatctatcaa aatagatcat agtctaacat
    47161 ccacatgtca cagcatctaa cttttctact cattcatgtg tctgcttcca tcaattttag
    47221 cataactaaa tcttgtttta catagaaaac cgattcctag cctattttta tacttactat
    47281 tttttcaaag aagacaagtg tatgcattcg ttaaaaaact gctgatagac catctcttca
    47341 tcaatcatag gaaaaagcca ttccctttta tgttctttta tttggcaaag ccatgagttg
    47401 aaatcaggaa ctttctggtt ttccaaggct tcttcgtaca acattcgata aagaaaaacc
    47461 tgttcttgta aataaaagaa aaaccgactt ttttgataga tccacgatga tagatgcaaa
    47521 agatgtttat gacgcaagta gcattgttct tgtatggata ctgtattctt ttgcttgtga
    47581 ataagttggc gtgtcagcca tttcttccca tcttcactca ctttttctat ttttttcatt
    47641 tggttcattt taatttcgtt actagtgaat atttctttaa ggtccctact agaaaaagtc
    47701 cagcagctac tgcaaaagga aatatgccct gatacagtct ctgtaatatg atgatacaaa
    47761 taaagctttt gtttcttgag atcagctttc cagcaatgca ctcctctttt tctattatag
    47821 tagcagcaac ttttttctat caatgagaat tgcgattgtc caagaaaaga attaccaagt
    47881 atccaccata cttggtaact gttattcaaa taattttggg ttctttcaat aaatctctgg
    47941 tgagacagtc tagaacattg gatttctatt gctagattcg ccaacaaaat atccggcctt
    48001 tgccgcaacc tcggcaaata ggcttccaaa aatactggtt ctactgactg tcccagccaa
    48061 tccatgaagc attcctttaa ctgtaagtgc tcttttgatt ctctttcact aaagaccgaa
    48121 cagttatctg atttttgatg ggcaaaatga gcacattttt tctttccctt tttcaaaatc
    48181 actctttttc gacagccacg acaataatat ttctttcctt tttctgcatg tttagcaaaa
    48241 cagcacttat tatattcatt cattgcaagc agcatttttc tccctctgaa cttatttacg
    48301 tctaagaatt tatttacacg aaaaaagagg gcgtgaaaaa gctattctgc atcaagtaat
    48361 aagaacagct aaacaaaaat agttcccaat atttttcgtt tggttgggct tgttaccgca
    48421 gatgcaagct tttgaacaca tttatacgaa aaaaagactg cgacacaatt tttgtcacag
    48481 tctcttacta taatgctgat ttatacaaaa taaaatttcg ttcgctcgat agcattacgc
    48541 gccatcagac attgtccata ttcattcaat gtttctggtg tacgatcgct cttttcagca
    48601 aactccaaaa ttcgagcaat ctgattctct acatccgctt ggttgagatt ttccatccag
    48661 aagtgaacga ctaggtaata gcgctcttcg aataaataca aatcagtcca agcagactct
    48721 agttcgacat ctgttgcaag ttggatcact tgttcaaaat cttcaaaagc aaaagttgct
    48781 tgtctttcct gttcttctag ggcgcttcca gataattctt cctccggatc gctgccaata
    48841 atctgctgac gaagaaagtc actcacatct tctgaatcaa cagtagaatc ttctgattga
    48901 gaaatttctt catccggaag atttttactg ataaacagtt ctaatccgtc tccttttggc
    48961 aaaacttgga aagtgacagc ttcgcttcct cggaactcct cttcgatatc aacttcttcc
    49021 aaaatactat aaaagaaatt ttcgatttct ttgtgattgc caagtaaatc caaaaatgaa
    49081 acgccccgtt cctctaaatc ttcatgacca attagtacac ggatcgtatt ttcattaata
    49141 tgttccattt ccattttgct acacctcact ttgccacaat gtttaatata gattcattgt
    49201 agcgaaatca gcttaaatgt aaagaaaaaa aatctcgctg ttaagcgaat ttaataaaac
    49261 cagcggattt ttcaaccttt caagctatct tatttaacta taacattttt ggcagacgaa
    49321 tactatatag ccgacttttc agctaatttt ccctctctat ttttcgtgca atcatcatct
    49381 tctactacct ttttatacat ctaatcaaga atactttttg taaaaacaaa aaaacttgga
    49441 aaaactatct ttgtaatagt cttt
//
LOCUS       DASJPH010000008        44652 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJPH010000008 DASJPH010000000
VERSION     DASJPH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.008"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            447..1637
                     /locus_tag="U3995_000442"
     CDS             447..1637
                     /locus_tag="U3995_000442"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1795791.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1739..2692
                     /locus_tag="U3995_000443"
     CDS             1739..2692
                     /locus_tag="U3995_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1795792.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2761..4110
                     /locus_tag="U3995_000444"
     CDS             2761..4110
                     /locus_tag="U3995_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1795793.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4293..4616)
                     /locus_tag="U3995_000445"
     CDS             complement(4293..4616)
                     /locus_tag="U3995_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795794.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4745..5908
                     /locus_tag="U3995_000446"
     CDS             4745..5908
                     /locus_tag="U3995_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1795795.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5978..7327
                     /locus_tag="U3995_000447"
     CDS             5978..7327
                     /locus_tag="U3995_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1795796.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7372..7803)
                     /locus_tag="U3995_000448"
     CDS             complement(7372..7803)
                     /locus_tag="U3995_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1795797.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8191..10833
                     /gene="alaS"
                     /locus_tag="U3995_000449"
     CDS             8191..10833
                     /gene="alaS"
                     /locus_tag="U3995_000449"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1795798.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11033..11629
                     /locus_tag="U3995_000450"
     CDS             11033..11629
                     /locus_tag="U3995_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1795799.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11751..12899
                     /locus_tag="U3995_000451"
     CDS             11751..12899
                     /locus_tag="U3995_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1795800.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12937..13350)
                     /locus_tag="U3995_000452"
     CDS             complement(12937..13350)
                     /locus_tag="U3995_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1795801.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13429..13860
                     /locus_tag="U3995_000453"
     CDS             13429..13860
                     /locus_tag="U3995_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1795802.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13959..14192)
                     /locus_tag="U3995_000454"
     CDS             complement(13959..14192)
                     /locus_tag="U3995_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1795803.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14375..16045
                     /locus_tag="U3995_000455"
     CDS             14375..16045
                     /locus_tag="U3995_000455"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1795804.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16192..16578
                     /locus_tag="U3995_000456"
     CDS             16192..16578
                     /locus_tag="U3995_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795805.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16565..17263
                     /locus_tag="U3995_000457"
     CDS             16565..17263
                     /locus_tag="U3995_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1795806.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17268..17732
                     /locus_tag="U3995_000458"
     CDS             17268..17732
                     /locus_tag="U3995_000458"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1795807.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17729..18640
                     /locus_tag="U3995_000459"
     CDS             17729..18640
                     /locus_tag="U3995_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1795808.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18875..19414
                     /gene="pyrR"
                     /locus_tag="U3995_000460"
     CDS             18875..19414
                     /gene="pyrR"
                     /locus_tag="U3995_000460"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1795809.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19428..20711
                     /locus_tag="U3995_000461"
     CDS             19428..20711
                     /locus_tag="U3995_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1795810.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20724..21650
                     /locus_tag="U3995_000462"
     CDS             20724..21650
                     /locus_tag="U3995_000462"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1795811.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21669..22949
                     /locus_tag="U3995_000463"
     CDS             21669..22949
                     /locus_tag="U3995_000463"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1795812.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22954..24030
                     /gene="carA"
                     /locus_tag="U3995_000464"
     CDS             22954..24030
                     /gene="carA"
                     /locus_tag="U3995_000464"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1795813.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24035..27220
                     /gene="carB"
                     /locus_tag="U3995_000465"
     CDS             24035..27220
                     /gene="carB"
                     /locus_tag="U3995_000465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1795814.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27274..28050
                     /locus_tag="U3995_000466"
     CDS             27274..28050
                     /locus_tag="U3995_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1795815.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28047..28979
                     /locus_tag="U3995_000467"
     CDS             28047..28979
                     /locus_tag="U3995_000467"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1795816.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28976..29692
                     /gene="pyrF"
                     /locus_tag="U3995_000468"
     CDS             28976..29692
                     /gene="pyrF"
                     /locus_tag="U3995_000468"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN1795817.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29699..30328
                     /locus_tag="U3995_000469"
     CDS             29699..30328
                     /locus_tag="U3995_000469"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN1795818.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30316..30630
                     /locus_tag="U3995_000470"
     CDS             30316..30630
                     /locus_tag="U3995_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795819.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30668..31309
                     /locus_tag="U3995_000471"
     CDS             30668..31309
                     /locus_tag="U3995_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1795820.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31352..32044
                     /locus_tag="U3995_000472"
     CDS             31352..32044
                     /locus_tag="U3995_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1795821.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            32041..32943
                     /locus_tag="U3995_000473"
     CDS             32041..32943
                     /locus_tag="U3995_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1795822.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="U3995_000474"
     CDS             complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="U3995_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1795823.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34997..35620
                     /locus_tag="U3995_000475"
     CDS             34997..35620
                     /locus_tag="U3995_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795824.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36070..37119
                     /locus_tag="U3995_000476"
     CDS             36070..37119
                     /locus_tag="U3995_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1795825.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37116..37997
                     /locus_tag="U3995_000477"
     CDS             37116..37997
                     /locus_tag="U3995_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795826.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38000..38770
                     /locus_tag="U3995_000478"
     CDS             38000..38770
                     /locus_tag="U3995_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1795827.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39049..39903
                     /gene="aroE"
                     /locus_tag="U3995_000479"
     CDS             39049..39903
                     /gene="aroE"
                     /locus_tag="U3995_000479"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1795828.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40081..41244
                     /locus_tag="U3995_000480"
     CDS             40081..41244
                     /locus_tag="U3995_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1795829.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41361..41771
                     /locus_tag="U3995_000481"
     CDS             41361..41771
                     /locus_tag="U3995_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1795830.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41945..44173
                     /gene="topB"
                     /locus_tag="U3995_000482"
     CDS             41945..44173
                     /gene="topB"
                     /locus_tag="U3995_000482"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1795831.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44344..44628
                     /locus_tag="U3995_000483"
     CDS             44344..44628
                     /locus_tag="U3995_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795832.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT        14617 a         7759 c         9728 g        12548 t
ORIGIN      
        1 tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc gaattcaaca
       61 agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata aacgaagatg
      121 gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc agaatacaat
      181 tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc caggtagagt
      241 aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt gaaagtaaaa
      301 agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa gcgttatcaa
      361 aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta tgttctgcaa
      421 gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg tatttagaga
      481 aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata ctagaaagtg
      541 aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca tccaacaatt
      601 atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca accgaaactt
      661 atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc catcagcaat
      721 tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt caatctggat
      781 tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct atcttatcgg
      841 atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca aaaatcatcc
      901 gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg atagaaagta
      961 agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat ggagacattg
     1021 cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc tacgtcgatg
     1081 atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat tttggattaa
     1141 gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt gttgggggtt
     1201 atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg ccattcctat
     1261 tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt actttgatgc
     1321 aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt aaagaagaat
     1381 tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca gtcattttgg
     1441 gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg atttatgcta
     1501 aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat atgccgactg
     1561 cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag
     1621 aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag agaacatgtt
     1681 cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga ggatcatcat
     1741 gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga tcacacggct
     1801 acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat
     1861 tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat
     1921 agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt cagctgttgc
     1981 agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca atgcattgga
     2041 agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc
     2101 aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa ccatgtatgg
     2161 ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat atggcgtaga
     2221 tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag gaggcggtac
     2281 gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt acactagttt
     2341 cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg atgccattat
     2401 aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca atatatcggc
     2461 gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac cagattttga
     2521 aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc caaattcact
     2581 agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact tagatgtgat
     2641 gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat aaaacaagaa
     2701 ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag gggacaaggg
     2761 atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt
     2821 acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg tgcaggggta
     2881 ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca tatgttttct
     2941 tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga actggcagct
     3001 gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg aaatacagca
     3061 gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt agcagccttg
     3121 tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc aatgattgtt
     3181 cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg ttctaaagct
     3241 ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc
     3301 attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat ccaagcagga
     3361 gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat gttcgcatac
     3421 gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc gaaagatttg
     3481 ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt agtaaatgcg
     3541 gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac
     3601 gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg tatcttgatt
     3661 tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc ttatgttatg
     3721 gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga taaaactaaa
     3781 gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat gatgatcggt
     3841 ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta tacgatggtc
     3901 tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt
     3961 ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat cgtatcaagc
     4021 acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact tgctggtatc
     4081 ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat tagaactgca
     4141 cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa atgtcaaaag
     4201 tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa
     4261 gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa ctgaactatt
     4321 cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa aatgggaagc
     4381 caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt cattttatcc
     4441 atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa acccgaaata
     4501 atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata agcgatcgta
     4561 gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt cagcatctcg
     4621 acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa tagcctcgtt
     4681 acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat caaggaggag
     4741 tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc catttttagc
     4801 cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa catttctagt
     4861 aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt attttttggt
     4921 gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa agatggagct
     4981 gcatttattt gcttcatggc aacatttcag agacaacacg gtattggtct tgtctgaacc
     5041 tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt ttaatgtttt
     5101 attactcata ccgtttggca tttatttaag atattatttc cggctttctt ttatgaaaac
     5161 gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa gtggacttta
     5221 ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga caaatacatt
     5281 gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc ctacaagaag
     5341 acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc gacgcttggt
     5401 cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg cgattttctt
     5461 taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt tattagaagt
     5521 aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg gcaaaaaaat
     5581 tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag cattgatgat
     5641 ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc agttgcttca
     5701 gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat tcatttctct
     5761 cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat ttttgagaaa
     5821 aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca cgattggcca
     5881 aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg tgttataatg
     5941 ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg atatattccg
     6001 cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt
     6061 ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc aatctcaaac
     6121 tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa
     6181 agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac agatctatga
     6241 agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga attttgtcgg
     6301 cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac aggttgtagt
     6361 aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc
     6421 atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt taaatgaagt
     6481 agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt ctgccactat
     6541 tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca ttgaagaaat
     6601 caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa
     6661 agatgagaat caaatgattt atcaactgct gacaattggt catccttatt tagcaatcgt
     6721 atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa accaaggctt
     6781 gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag tgatgcgaca
     6841 agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta gagggatcga
     6901 tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag acttctttat
     6961 ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta cgttatattc
     7021 gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa
     7081 agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga
     7141 aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga aaaagaaaaa
     7201 gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga
     7261 acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga aaaattcaca
     7321 taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag
     7381 actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg tgcaagtatg
     7441 atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat aactagtctg
     7501 acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc ctaacccttt
     7561 tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt
     7621 ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat aggtttgttg
     7681 cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta atacaaaaac
     7741 ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa ctattttttt
     7801 catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat tcttatcgta
     7861 ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat attgacagtg
     7921 atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt tttaagactc
     7981 acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc tccctttttc
     8041 tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt aatgattaca
     8101 catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt
     8161 gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc acaagttcga
     8221 caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag tgcgtctttg
     8281 atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac attgaaaaaa
     8341 tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca aaaatctatt
     8401 cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat gtttgagatg
     8461 ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg ggcatgggaa
     8521 tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt aacagtttat
     8581 ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc actagatcac
     8641 attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg tccggatacg
     8701 gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga tcctgaaaac
     8761 tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt ttcagaattc
     8821 aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga tacggggatg
     8881 ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga gacagacttg
     8941 tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac
     9001 ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct ttcatttgct
     9061 attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg ccgtttgctt
     9121 cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt atatcgttta
     9181 gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca acaaaaagaa
     9241 tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat caatgaaggt
     9301 ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac gactctagca
     9361 ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt aacggaagag
     9421 gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga aatggatgca
     9481 caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt acaatctgct
     9541 ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca agaatcagat
     9601 agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc agaaggtgaa
     9661 gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca agtagcggat
     9721 caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa
     9781 gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag tgaaggacaa
     9841 acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca
     9901 acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa tcaagctggt
     9961 tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca aatcacttca
    10021 gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt
    10081 gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt
    10141 gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat cgaactgtgc
    10201 ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt ttctgaatca
    10261 ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta
    10321 cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc acctcaattg
    10381 aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct acaaaaggaa
    10441 aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt caaagatcca
    10501 gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga tatgaatcag
    10561 ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct tgttttagca
    10621 aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc aaatgaagct
    10681 ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg tggcggtggt
    10741 ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc agatgcactt
    10801 aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa
    10861 agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc ttgccctcat
    10921 acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg tctttagttg
    10981 acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa aaatgaatag
    11041 tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca
    11101 agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa caattatcca
    11161 tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg tgattggctt
    11221 gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc catttgatac
    11281 attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg gcttagtagc
    11341 aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca gctcagcctt
    11401 tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca tgggattatt
    11461 ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat ttaaaacctt
    11521 agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa tcataacacc
    11581 attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat gaccatcttt
    11641 taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa aacaatcgtc
    11701 ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac ttggctaaga
    11761 aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga gtactagcgg
    11821 gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg atcgggcttt
    11881 tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat actgcttcat
    11941 ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag gaacatcctt
    12001 atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc attacttatg
    12061 taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa aatgtctctt
    12121 taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt gcacaaggca
    12181 aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct acagcaacag
    12241 atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca atcgaatggg
    12301 taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat atcctttata
    12361 gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc ccaacgccag
    12421 cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt ggctatcatg
    12481 atttattggt gcataactat ggacccaata aacgctttgc atcagtccac atcgaagtag
    12541 atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa aaagacttta
    12601 aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt gataacgaaa
    12661 aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt acagggctta
    12721 atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat gtagtggtcc
    12781 ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt caaatagaag
    12841 aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt ttatattaaa
    12901 tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt tttcttttaa
    12961 ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt tttcgggcaa
    13021 ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt gaaggtaggg
    13081 aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc
    13141 tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt
    13201 taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga tagatagctg
    13261 ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag ggttgccttt
    13321 tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt gttcaaacta
    13381 atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat gaaaaaaaat
    13441 attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac cttgatcatt
    13501 ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa aagcaatttt
    13561 gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt tattttaaaa
    13621 tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat atctgtcgga
    13681 cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca agatgagaaa
    13741 gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa
    13801 aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag aaaaagctaa
    13861 ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga gtgagacgta
    13921 cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat actagtatcg
    13981 gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg
    14041 atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc ttcaatcgca
    14101 gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta tccttttttg
    14161 ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct aaagtctgat
    14221 catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa acctgtaatt
    14281 ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa agaagatatt
    14341 ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca
    14401 agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta aggaagacga
    14461 tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca atcgatcaaa
    14521 ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac cagctggcga
    14581 aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca ataaaaaatc
    14641 tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc
    14701 aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt ttactggtga
    14761 catgcatgct atcacaacag ctaataatgc attatctgct cttttagata atcatattca
    14821 tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct
    14881 caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc aaggtgttcc
    14941 aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct tgtgtcttgc
    15001 ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct atacatttga
    15061 ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat tactgctgaa
    15121 agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt ttgtccacgg
    15181 gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca agacagcatt
    15241 gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg gcggagagaa
    15301 atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc
    15361 cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa
    15421 tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta atatggaaca
    15481 atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag acaatgaact
    15541 cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag cgagtgtatg
    15601 ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat tgattgatag
    15661 aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag aaaagacaga
    15721 aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa
    15781 acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta tggcaaaaac
    15841 acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt tcaccatcac
    15901 gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga ctggagatgt
    15961 tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg tgacaaatga
    16021 cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag gagctgtatt
    16081 ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac taggatcatt
    16141 ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat gatgaaaacg
    16201 acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct aagaatcaac
    16261 aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct ggaagtgccc
    16321 tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt gaaagaagca
    16381 aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc gtttttattt
    16441 ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt ttttgcagtt
    16501 attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat caaggaggaa
    16561 aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa aatggatgag
    16621 agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac gcctagctca
    16681 aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg ctcagttaag
    16741 gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc caaatgattg
    16801 gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc
    16861 tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg aactgttaaa
    16921 gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa aatttgtagc
    16981 attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt
    17041 tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt acacaaatta
    17101 tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc gtgagcaagg
    17161 gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc tgctcgggat
    17221 cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg ttaattattt
    17281 attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg tggatcactg
    17341 ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca ctgaatcata
    17401 ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc tttactatag
    17461 tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt gaaaaaggta
    17521 atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc aattttatcg
    17581 accgtattcg tcttggctat gtagtagata tgttccagac agattttatg agctttccga
    17641 tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta atttatatta
    17701 ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga ttcaccatga
    17761 atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc gttcgcaaat
    17821 tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca aagcaaatta
    17881 caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa cattagatgt
    17941 ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag cggtgataaa
    18001 caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca caatggtcaa
    18061 tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc gtccaggtat
    18121 tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa atgaccaggc
    18181 acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat atgttgcttt
    18241 agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa
    18301 ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca cacattttac
    18361 tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa ccggaagaac
    18421 acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag atccagtata
    18481 cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt
    18541 tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag aagtttttga
    18601 aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac ggtatagtaa
    18661 ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg tgaggctaag
    18721 aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt gacatagtat
    18781 agctatcagt gtatcagtat aagctcagtc taaacattcc tcctgtcctc ttagggcagg
    18841 agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag tagtagatca
    18901 ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga gaaaccatag
    18961 tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg catcaagaat
    19021 tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac tggatatcac
    19081 tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt cttctgacat
    19141 cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt atacaggacg
    19201 aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa aaatttcatt
    19261 agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt atgttgggaa
    19321 gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc ttgatggaca
    19381 agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg tcggagaaaa
    19441 aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc ccaggacatt
    19501 gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg ttagtcccca
    19561 tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt acattggtgt
    19621 acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc gctttcattg
    19681 cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa ggagcagtca
    19741 caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt tcagcatggc
    19801 tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc gggttgggac
    19861 ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat ttcaagtata
    19921 ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg ctaagaggtt
    19981 ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta gccttgctgt
    20041 ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt cctaactttg
    20101 agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca acaatggcac
    20161 ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg aataaactaa
    20221 ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt gatggattag
    20281 cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt gaaaacattg
    20341 gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct gcagtatttg
    20401 cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc cctggaccag
    20461 tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga atgaagattt
    20521 taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct tcagttatct
    20581 tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg tcggcaatgg
    20641 cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact tcacgaagcg
    20701 aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc ttaaagcatt
    20761 tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc agagggcagg
    20821 aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct acaaacctct
    20881 ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa aaactagggc
    20941 tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa acactatacg
    21001 atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt catggtgatg
    21061 agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt atcaatggag
    21121 gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc atttatgaag
    21181 aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact cactctcgcg
    21241 tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc ttctcaggtc
    21301 ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca ttggatgaac
    21361 tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc catgatggaa
    21421 aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt gagcgtgcga
    21481 agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt gatgtagaat
    21541 tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg agcaatggag
    21601 tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg
    21661 atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa aatatgacga
    21721 caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca ggattctcag
    21781 aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct ggtttagtgg
    21841 acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc gaagctggga
    21901 ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg
    21961 tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa gatgcagttg
    22021 tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc
    22081 aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa
    22141 cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa gccctagtag
    22201 cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc aagaaggcag
    22261 aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt gctagagatc
    22321 tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct actaaagaaa
    22381 gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca gaagtttcac
    22441 cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt tggaaaatga
    22501 acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg cttgacggta
    22561 cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa agccaaagtt
    22621 tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg atctatactc
    22681 attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg gcagttaaac
    22741 cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc gctgatgtag
    22801 cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta tcaaaagggg
    22861 agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg
    22921 gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat attagaagat
    22981 ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt
    23041 tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt taacggtcaa
    23101 atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga tgattatgaa
    23161 tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt agcttcaaac
    23221 tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc tggaatatca
    23281 ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat gaaagctagt
    23341 atagttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc aaccgttcta
    23401 gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc aggaacaggt
    23461 cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga attgagtaaa
    23521 agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat tttagcgcta
    23581 tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt gccagaagca
    23641 atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac
    23701 cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg acatcgagga
    23761 ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc tcaaaatcat
    23821 ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa
    23881 gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt cagtgtgcag
    23941 tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga tgaatttatg
    24001 gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag atatcaagaa
    24061 aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat ttgactacgc
    24121 aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt tagtaaattc
    24181 taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata tcgaaccgat
    24241 cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc tccttccaac
    24301 tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag gtattttaga
    24361 agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag cagaagatcg
    24421 tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt
    24481 caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg tcatcgttcg
    24541 tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag aagaattacg
    24601 tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga ttgaacgaag
    24661 tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg ataatgcaat
    24721 cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg attctatcgt
    24781 atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg atgcttctct
    24841 ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg ctcttgatcc
    24901 acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt catctgcgtt
    24961 agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag cgcttggatt
    25021 aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat ttgaacctgc
    25081 attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg aaaacggaga
    25141 acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg gtcggaatat
    25201 cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc atatcgaact
    25261 tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc gagcacaaga
    25321 tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag aagaattagc
    25381 ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg tagaaatcga
    25441 acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga aacaaaacgg
    25501 ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg ttcgccagat
    25561 gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg cagcggaatt
    25621 cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa gttcacgatc
    25681 agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac aaggggttga
    25741 atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat acgaagcaat
    25801 catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg ataaattata
    25861 ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac agccgatagg
    25921 agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt tgactgctgc
    25981 tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa atcgtgattt
    26041 attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata ctgctacaag
    26101 taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg ttcgtccaag
    26161 ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt tagaagatta
    26221 catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc gttacttgct
    26281 tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca tcccagggat
    26341 catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg tgtatccacc
    26401 tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc gcttagcatt
    26461 aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa atgaggttta
    26521 tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta aggtgacagg
    26581 aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa agacacttgg
    26641 ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac ctgtcttttc
    26701 ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat cgactggaga
    26761 agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg aagcttctgg
    26821 attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa caaaagaaga
    26881 agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag gaacgaaaca
    26941 tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga aaatttctga
    27001 ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac aagtggttgt
    27061 caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca tccgaagaga
    27121 agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg cgatcgttcg
    27181 agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac atgttaagac
    27241 aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat ggaaatcgtt
    27301 cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga tctagtaaaa
    27361 gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga cttgctatta
    27421 cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg tatcatttat
    27481 cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga ccatttagat
    27541 gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg agacgaagta
    27601 tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa acagttagtc
    27661 gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt cgtttattat
    27721 gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg aacatacggt
    27781 atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac agctgttttt
    27841 gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc gcatcaaaat
    27901 gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt
    27961 tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg
    28021 atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc aaacttcctg
    28081 gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt ggcaaagaat
    28141 acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca acgactccaa
    28201 atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc atgttgaatg
    28261 caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt ccagaattag
    28321 aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg
    28381 tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt
    28441 cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat gttgcttttc
    28501 aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa
    28561 atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca gatgggttta
    28621 cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag
    28681 ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt cgtttgattc
    28741 accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg cagacagtgg
    28801 atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg accgcgaatt
    28861 tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga atgagcgaac
    28921 taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt gaacgatgaa
    28981 tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag aggtagaaga
    29041 ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt
    29101 ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata atgtattttt
    29161 agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa
    29221 gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga tggaggcggc
    29281 aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac ttctagctat
    29341 cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc
    29401 tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat tagatggtgt
    29461 ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg aatttgtttg
    29521 cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa gagtagtcac
    29581 accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac caatcactca
    29641 gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct aacctaaaat
    29701 gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat ttttaaatcc
    29761 aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg ataaccgaat
    29821 cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa
    29881 agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa tccctcatgc
    29941 cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta aggcgaaaga
    30001 tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat
    30061 cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag caaaaagaga
    30121 aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa aaggaaaagc
    30181 aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg tattgattga
    30241 agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa aagaatggaa
    30301 aaaagatcct gaaaattggc agatttaagc gcgcttgatg aagatagaga aatccgattt
    30361 gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc gagaagcatc
    30421 ttttcatcag gtatgaaaag aaacttctat cgtgatcagt ttttgatttt tttaaaattc
    30481 atttttctaa gactggttag cagaaagaag agaataatct cttctctttt ttctattata
    30541 tatagatttt ctctattttt tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat
    30601 gttgcgaata tcagttattt aatgatatga tattcggaaa aagaaatctc aaagggaggt
    30661 aacttttatg gggttgaatg aatttatttt aggattgaac aatttagaga cgatcacaag
    30721 ggcacctgga ttttttaaat atacagagca tacagttgca gcacattcgt atcgtgtagc
    30781 atctattgcg caagttctag gagacattga agaagcgaat ggacaaccga tcgattggaa
    30841 actgctctat gaaaaatcat tgaatcatga ctacacagag cgttttattg gtgatatcaa
    30901 aacgccggtg aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa
    30961 gatgacggac gaattcatca accaagagat tccagctgag ttccaagaag tttatcgccg
    31021 tcggttgttc gaaggaaaag atgaaaccgt agaaggagaa ctattagcca tttcagataa
    31081 ggttgacctt ctttatgaat cttttgatga aatcatcaaa aataacccgg aaaaagttta
    31141 taaggagatg ttcattgagg cagtgactac gattaaaagc tttgaacatc ggccttctgt
    31201 tcagtatttc tttaaagaga tatttccgga actattgaac caggaatttt atggaaaaga
    31261 ggcgttttta gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa aatgatatga
    31321 gatgcagggg gaagtatagt tgaaaaagaa tatggatgtc gaatggggct attctggaga
    31381 caaaggtccg gaaaattggg cgagtttatg tgattggttt gcgagaggtg cggaatttcc
    31441 attgcagtcg cctattcatt tgaccaaggg ggaagaaacc aaaatagaag gagacacgct
    31501 ttcctttcat tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca cgattcattt
    31561 agtacctttt gaccaactca gttatgtaga gttcaagaaa gaacgctatt atttgacaga
    31621 tattcatttc cacttgcctt ctgaacatat catcaacgga aaacaggaag atattgaatt
    31681 tcattttgtt catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct
    31741 aatggtacag gaaggttggc tgtataatca agaaaatggg gaaagttgga acttagaaaa
    31801 acatgagcat tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc atttccatta
    31861 tattggctca ttgacaactc cgcctaccag cgggccaatc caatggtttg taatggatca
    31921 tgtaggaaaa ctgaaccaag agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa
    31981 caaccgacca atccagccct tgaatggaag agaaatttat tatttcgagg agtttgagca
    32041 atgaatattc agcagatgaa atacgtggtc gctgtagcaa acaatggtag tttcagagaa
    32101 gcggcaaaga aaatgtttgt ctcccagcca agtctttcag ctggtatcaa agaattagaa
    32161 accgagttag ggatcacgct gtttactcga acgaatcgag gggcatattt aacagaagaa
    32221 gggcaagact tcttaaatcg tgccgagaaa atattggtcc agcttgatac attagaaact
    32281 cactatctaa cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca ttatgatttc
    32341 ttaggtcctt tgaccgcaca gctactcgag gaattcaaag acgagatcaa acaatttcgg
    32401 atcatggaga cgacaacagc gagagtgatc gaagaagtca aagagcagca cagtgagatc
    32461 ggcattattt atatgaatga gcataatcaa gctggtatca gaagatattt acaacaagga
    32521 gagctaagtg gccgcagcat tggaaaattc aaaacgcata tttttttaag gcaaggacat
    32581 ccattagctg aacacgcaga gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt
    32641 tttacacagg atggaaataa tttcccgtat ttttatgaag accttatcga gatccctgat
    32701 caagaaacag tgatttatac gagtgatcga ggtactttga tgaatatcgt tcttgaaact
    32761 gatgcttacg cttcaggatc tggtattgtg attggaggga ttcgcgagca cttgcggtta
    32821 atccctttgg cagaaggcga actaaatgaa ttttatatca tttattcggc gaaacgtccg
    32881 ctcagtgaaa tcgctcaaag atttatcaaa gagcttactc ggttactgga ccagcagagc
    32941 taagaaaagc agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt
    33001 gacagaattt tgtcacaact cctctttttg tagcaaacaa tttataacta attgctgttg
    33061 tgggaaagta cgcaaatatg attctagaaa cactcattga ttacctgttt tgtttcatag
    33121 ctgtaatttt ttcttcttct ggtgtcacga taatcgtctt ttggttttca taaataacat
    33181 aaccaggctt ggcgccattt ggttttcgga tatgctttac ctgtacaaga tcaaccggca
    33241 cttgggcaga gtatcggtat tttgaaaaat aggcagctaa ctcggcagct tctgtaattg
    33301 tttcatctga gggcttgtca cttttaatga tgacgtgcga accgggtata ttttttgcat
    33361 gaagccaata gtcggttttt ttagctgtct tcattgtcag ctggtcattt tgaagattgt
    33421 ttttaccaac aagaatgagc gttccgtcag aagaaaagta ttgatcaggc tgagatggtt
    33481 tcttacgttt ttgttttttt aagttcttct ttttcaggta tccttctgaa gtcaattctt
    33541 ctttaattgc ttcgatatcc attgggccgg caatttccag ttgagataag acagattcaa
    33601 gatattggat ttcatctttg gcttcttgga tttgttcgcc aatcagctta acagcgttct
    33661 tcagcttttg gtatcgatgg aaatatttct gtgcattttg atttggtgtc aatgccggat
    33721 ctagtgctat tttgattggc cgatcttctt cataataatt aggtaaaaca acttcttttg
    33781 cgccgcgtgg tacttgtgtc agaaaagtag taagtagttc gccatttcta cggtaattct
    33841 cagcgttttc ggattctttg agtgtttgtt cacgtttttt caatttattt ttatttcgtt
    33901 ttagttcatt ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc cgatctttct
    33961 ctgctttttc gtgataatag ctatctaata attgactgag tgttggataa gctgttacag
    34021 cagatgcctg ttcggacaat acctgataag caatcggtgt gaaaaaatct ttattttccg
    34081 tttcgtaaaa agtaggaata ggctgatggc tgatggctga gaaaaattcg tcccatacaa
    34141 ccatcttttc attaggacgt tccagtaatc ttttggtcag ctcttgagcg atatcgaaac
    34201 cgattccctg aaactgacgt tggattgctt ttggatctag ttcagtttgg gaaagccgct
    34261 gaaagatttt ttctttctcg gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa
    34321 catattctac accaggaagc aaagaacgat aagtgttttg agagcttcca atgtgtttga
    34381 tcgcatccag tatttttcca gtttctcgat taactaagac gatcgtacta tgacgtccca
    34441 ttaattcaac gatcaatatg atattttgta gatcacccaa ctcgtttcgt ttagcaaagt
    34501 ggaaatggat cactcgatca ttttcgattt gttcgatgtc ttctaaaata gcgccatcca
    34561 aatattttcg aagcatcatc acgaagttag gcggattatc aggattctga taatcgattt
    34621 gggtaatctg tacacgagca taacttgggt gcgcagataa taataatcga tggtttttgc
    34681 cgcgtgaacg aatcaccagc acgatttcat tttcataagg ctgatggatt ttcgagatcc
    34741 ggccagatag taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa
    34801 aggacatggt tcattcctca tttcttttca taagtatatt cattataacg aaaaaaagaa
    34861 gaaaaaccaa tttggccgaa tagtaaagca aaagtctaga aaaatcttgg actaattaca
    34921 taaaaaacag tgtttctatc gttgaaatcc aaatttttag gtataataaa aaataggaga
    34981 aaaagtagaa aagaaggtgt ttgttatttg ggaagtagtt aaatcgagag tacaagaaat
    35041 gaatgaacat ttctcagtgt cattcgcaga aaatccaagt aagtattttc gacgtcttgt
    35101 ttttctgctc atcttatcat tgctgttaac agttgcaatg tttttattgc cggaaaaaat
    35161 ccgagaagag caagtaaata gtctcgttta ttcgtccata ccaaccagca aattgaaacc
    35221 ttttgattac agcaaagctg atcagaaaat caaacaaagc cgggcgatat cggtgatgtt
    35281 cagcaaaccg aacgggaaaa cctatcagga catgcttgat gtttttaatg atcctgagct
    35341 tatggaagaa ttgaatcgac cgatctttta ttatccgatc gtttatgatg tacatgaact
    35401 ggaacaaaaa tataacatac gaacggatga agtgacattt attttctttg atggaggaaa
    35461 agaagcaaat cggataactg cgggtaaggg aggaataact gattttgata aggagttgat
    35521 ccctgaactg aatcgacttc cgttagccaa tatcaaacaa ttagaagaag aattggatag
    35581 aacgggagta acttcccaaa attccgagat tgctaattga gacattggcg cgatgaaacg
    35641 gtgggtctat atagcgctat ttttaattat cagaacaagt gatgtttgat cttcaaaata
    35701 acaaataaaa aattaaaaaa tatagttgac gaaattcaaa aattcgatta gacttaaatc
    35761 aattcttaag gaaagggtga cctgtaatga acttatcaaa acgtatttca gcagaaaaca
    35821 aaaaaaattg gcgcagctgg cgtagataag ttgtattaat ggtgagactt tcccatagat
    35881 acaacttttt agagtgcatt tctaaagctt gtatctatgc tagacatgaa cattgactgc
    35941 cgcatagatt agaatctatg cggttttttg tgtaaagaca atgaccgctt tggccattgt
    36001 ctttttttgt agactggata cttgcctgat tataataaat aaaaagattg actttgcagg
    36061 aggaaaaaaa tgaaaaacaa acgattaata acagttgtag taattatttt agtttattta
    36121 gcaggtgcat ttatttatgg aaaagcctca ggcgaaaaag aaagtacttc gacgaaccag
    36181 acaaaaaaaa caacagttgt tggggtattg caatacgtga gccatccggc attagatgag
    36241 atttatcgag gaatcaaaga tggtctgaaa gaagaaggat taaaagaagg agaaaatctt
    36301 gagatcaaat ttcaaaatgg acaagccgat cagagcaagc tagccacaat gagtcaacag
    36361 ctagtacaat ctgatccaga tgccttgatt ggaatcgcaa cacctgccgc tcaatcatta
    36421 gcaaacacga cttcatcgat cccagttgtg ttaggtgctg taacggatcc tgtgggggct
    36481 ggattagtcg cttctttaga gaaacctggt ggtaatgtaa caggtgtttc tgatcagccg
    36541 cctgttactt cacaaatcaa gcttgcttct gatttgttgc caaatgcaaa aaaagtcgga
    36601 atcttgtatg cttcttcaga ggataattca aaataccaag tagcacaagc agaagaagct
    36661 gcaaaaaaag aagggttaga atcagtaaaa tatgcggtgc cttccactaa tgagattgca
    36721 caaacggttc aagtgatgag ccgacaagtc gattttatct atgttccatt agataatacc
    36781 attgccaatg cgatgcaaac agtggtaaaa gaagcaaaca aagcaaatat ccccgtcatt
    36841 ccatctgtgg atacaatggt ggaacaaggt ggactagcaa caatcgggat caatcaatat
    36901 caattagggc ttcaaagcgg taagatggcg gcagaacttg cgagtggaaa agaaaaaccg
    36961 gaaacgacgc ctgtttatac atttgatcaa ggggatacag tgatcaatca atctcaagct
    37021 gatcgcttag gaatcattgt tccccaatcg atgaaggaaa aagccaaaat cattacagat
    37081 gactcgcagc aagaaacatc taaggaggat gacaaatgat cgtatcagct atttcacaag
    37141 gcatgctttg ggcaatatta ggattaggga ttttcatgac ttatcggatc ttagactttc
    37201 ctgatatgac gacagaaggg tccttcccat taggtgctgc tgtctgtgtg acagctatta
    37261 ctcatggggt agcgccgatt gtagctacat tattaggcgt aggtgctggc atgatagcag
    37321 gactcgttac ggggttactt tatacaaaag gaaagatacc ggttatttta gcagggattt
    37381 tagtgatgtc aggattgaac tcagttattt tgtttgtgat gcgaacacca aatctttctt
    37441 tattgaatca tccgattcta caagacagct tccatcgctt tgggttacct gattactttg
    37501 acacagtcct tcttggcttc atctctttgg gcatcttgat tgctgtactg ctattcttct
    37561 tcaatacaga tttgggtcaa ggctatattg cgacaggaga caacgaaacg atggctcggt
    37621 cgttagggat aaaaaccgat cgtatgaaaa ttttaggttt aactttgtcg aacggtgtca
    37681 ttgcattatc cggtgcgctg attgcccaga acgacggata tgcagatgta aataaaggga
    37741 ttggcgtaat cgtcatcggt cttgcttcca tcatcatcgg tgaagtgatc ttccaagaat
    37801 tgacgctagc agaaagattg attgctattg ttgtcggcag tatcatttat cagctattga
    37861 ttttagtagt aatcaaacta gggtttgaca cgacttatct gaagttgttc tccgctatca
    37921 tcttagctat ttgtctgatg atcccgcaat tgaagaaagc actgaactta aaaacgggtt
    37981 ttgagaagga ggcatagtaa tgactgatat attgacaatc aaaaatggca gaaaagtcgt
    38041 aaacaatgga atgaatgaag agaaagtact attggatgaa attaacctta caatcagaga
    38101 aggggacttc atcactgtcc ttggcgggaa tggtgctgga aaaagcacac tgttcaatac
    38161 gatcgctggt acgcttcaac tcagtcaagg atcgatctct tttaaagatc agctgatcac
    38221 aaaagattca gaagaaaaaa gagcacgatt cctttctcga gttttccaag accccaagat
    38281 gggaacggct cctcggatga cagtagcaga aaatttgcta cttgcacaaa aaagaggtca
    38341 aaaacggaaa ttccgtttac gtaacttgaa aaatcaaaaa gaagattttt tcagactatg
    38401 tcaagaagtc ggtaatggcc tagagcaaca cttggatact ccagcgggtg aattatctgg
    38461 tggtcaacgc caagcattaa gcttgctgat ggcgacgatc acagctcctg agttactatt
    38521 attagatgaa catactgcag ctttagatcc taaaacagct aaagcattaa tgcgattaac
    38581 aaatcagcgg atcaatgaac aaaaattgac ttgtctcatg atcacgcatc gaatggaaga
    38641 cgccttgaaa tacggaaatc gtctgatcgt actccaaaaa gggaaaatcg ttcaagatat
    38701 ttctgaaaaa gaaaagcaac atttgacaat gggcgacctt ttgcagttct ttgaagaagt
    38761 agtggattga tttcgacaga aaaattcctt tcagtagaag gacttgaaaa ctcattgaca
    38821 tcttcctttg attcatccta tacttaaaac aataaacaat taaacatatt tgggtgtttt
    38881 gtaggatcac gtagtcaatg attttaacaa ttaaatagaa tattctttgc tctgacaagt
    38941 aaacgatgag aaaagaattt gctactttgc cacctagacg tttcgtactt tttcgtatca
    39001 aacgctaagt ggcttttttt attgaagtaa gaacaaagga ggaataagat gatttctgga
    39061 caaaccaaat tagctgggct ctttgctgat ccggcttctc acagtttatc cccattgatg
    39121 cacaatactg cttttgaagc aaacaacatc gatgcggtgt atttagcttt ccaagtaaat
    39181 ccaatgaatt tgaagcaagc agtcgaaagt attcgaactt ttgatatgtt aggtgtaaat
    39241 ctttcgatgc cgaataaaac agcggttatt ccttatctgg atgaggtaag ccaggaagcg
    39301 caattgatcg gagcagtgaa tacgatcgtg cacgaaaagg gtcgtttgat tggctataat
    39361 actgatggtg cgggcttcat gcaatccgtg caagaagctg ggatttcgat caaaaagaaa
    39421 aaaatgactc tactgggagc tggaggtgca gcaaaggcag tcactgttca agcagcgtta
    39481 gacggcgctg aggaaatcgt agtttataaa cgaaacaacc aaacattcgc tcaagtaaaa
    39541 gaaacattcg aacaactcag ctatcgaact tcttgtaaga taaaagttcg tgactttgcc
    39601 gatacgcatc aattaagaaa agacttgaaa gaaagctgtc ttctagtcaa tgcaacagat
    39661 atcgggatgg gaaagaaaat caatcagtca ccgctcttag atataagcat gatgcatgag
    39721 aatctagcgg tttttgattt gatttatgca cctagagaaa cacgtttatt aaaagaagca
    39781 aaaaaagtag gagcaaaagg atacaatgga cttggcatgt tgatctacca aggagcaatc
    39841 gcgtttgaat tatggacaaa ggaaaagatg ccgatccatg tgattgaaaa gctgtttgat
    39901 taaaaaagga taatagataa agatctttta ttctcgcata aacggtctca agaaaagtaa
    39961 tcctttatgt atcgaagcat ttatttcgtt attttttatt aaattatcaa aaaatgatag
    40021 gcttttgtcg attggttatc gtatccttta tttatcagta agaaacgagg aggaaccaca
    40081 atgagtcgga tggatcgcta taaggacatc cataagaagg cgaaacctct gaagcgggat
    40141 acggggagta tttttgctag aagagaacga aaacgaacgc gtatggaaaa tgaggaagat
    40201 gctacccgta tttatgaaag agagcagcat tcatttgaac atttgaacca atacgagcaa
    40261 cagatataca gagatgcacc aagagcagaa gaacaacaaa aaaataaaaa atttttcaat
    40321 cggcaaaaga agaacaaacc gttaaaacct cgaagaaagc gatcgtggaa aaaaatcatt
    40381 ctatcgattt tgcttgtttt gatcctttat tcaggtatct cattttttat tggtaaatat
    40441 gtagctgaac atgacacaag cttagaggca gcgacttcag aaacctttca tgggcaaaaa
    40501 gcgacgaatg gtgcgcataa tattttgatc cttggaagtg atacacgcgg agaagatgcc
    40561 gggcgagccg atacgatcat ggtcttacag ttggatggtc cttcccataa gccgaaactg
    40621 atttctttta tgcgtgacag cttcgttacg atcccagggg taggacaaaa taagatcaat
    40681 tcagcttatg catacggagg agccgatcta gtgcgtcaaa ctttagtaga aaattttgga
    40741 atcgattgtc aatactatgc gaaagtcgat ttcaaatcgt ttgaaaaagt gatcgatgcc
    40801 ttattcatga acggagtgaa gatcgatgca gaaaaagatc tgaatctgga tggtgtagat
    40861 attaaaaaag ggggccagaa gatggatgga catgttcttt tgcagtacgc acgtttccga
    40921 atggatgaac aaggagactt tggccgtgtc agacgccaac agcaagtaat gaacgctatt
    40981 ttcagccaat tgaagaatcc attgaatctg atccgtgcac cttatgcagc tggtaaagct
    41041 ttaggatata catccacaga tgtcactgct tggttcttga cgaaaaacct cttttcgatt
    41101 gcacgaggag ctggtggcat tgatcgaata agtgtgccag tagacggttc gtggaattat
    41161 gggcaaagtg attatgctgg cagtattctt gaaattgata atcaaatgaa tcaatctgct
    41221 attacagatt ttttgaataa atgatcataa aaaagatcaa gaagaagctg cgcaaggctt
    41281 ttgtcatgaa tcaatcaaac gattcactcg taaaaaacgc tttcttatgg tagatttaac
    41341 caagagagga gctgtttaca ttggatatct atctgaactt caaaaatgag tcgcgtgaag
    41401 ccatcgcttt ttatgaaaaa gtttttcaga ctgaagcatc aggtatcacc acatacggtg
    41461 aaatgcctgg aatagaacaa atgaacttgc cagaagaagc aaaaaatcta atcacgaatg
    41521 cacggctgaa catttatgat acattagtta tgtttgcaga tacgccagaa ggaattggtc
    41581 ctgaattagt tgtgggaaat aatgtttctt tagtcatcca gacaagaaat gaagaagaaa
    41641 cgaagcgcct atttgaaagc ttagctgagg gtggtaagat tgagatgcca ctggatgctg
    41701 ttccgtgggc tcagttgtat gggatggtaa ctgatcgatt tggcatcaac tggcagatta
    41761 accaagagtg acagctaaag atgaatattt tctggaaaca tcaagcagca aacggatcat
    41821 cgtttgtttg cttgatgttt ttgtgtctat ttcgactata cggtatcttt tttttattgc
    41881 cagatgcgaa aatatgttcc ataatagaga agtccgcaaa aaattaagta acggaggtca
    41941 gaagatgaag acggtgatac tagctgaaaa accttcacaa gcaaaagcct atgccgaaac
    42001 cttccaacag caaaagcggc atgaagggta ttatgaaatc agcgatccat tgtttgctgg
    42061 tgaagtaacc atcacttatg gttttggaca tttggtagat atggttgctc caggagctta
    42121 tgaagagcgt tgggcaaaat ggtctttaga aaatttgcct attttcccag aaaccttccg
    42181 ttacgaagtt ccaatggaca aacgtgcaca attttcgatc gtaaaacgag aattgcagtc
    42241 agccgatacc atcattattg caacagatgg tgatcgagaa ggtgaagcca tcgcgtggtc
    42301 gattattcga caagcaaacg cattttctaa ggaaaagaac tatttacgtt tatggatcaa
    42361 ttctcttgaa aaagaagcaa tctatgaagg ttttaagaat ctccaacctg gaaagaacta
    42421 ttttccaaaa tataaagaag cgcaagcaag gcagaacgct gattggctga ttggaatgaa
    42481 tggaagtcca ttatatagct tgctacttca acgaaaaggg attgatggca gtttttctct
    42541 tggtcgtgta caaacaccga cactctatat gatttatcag ctgcaagaag aaatcaaaca
    42601 cttcaaaaaa gaaacgtact ttgaaggaga aggtttaatt acgacaaaca aagggcagtt
    42661 ttctggaaaa atcgtaccaa aaaaagcatt taaaacccaa gaagagttaa cgaaggaaat
    42721 agaaaaactt ggagcgcatt taggtaagca aaacggacgg attcttgaag ttacgaaaaa
    42781 ggaaaagagg ttgcatagtc cacgtttatt ttcgctatct agtttgcaga caaagatgaa
    42841 tcagctaatg aaagcaagtg ccaaagctac acttgatgct gctcaaggat tgtacgaaag
    42901 caagtttcta agctatccaa gaacggattc cttttacatt acagaaaatg agcatcagta
    42961 tctggttaaa catctgcaaa attataaaaa atttttagga atagaagaag tacagacaac
    43021 tgagatcaaa gcaaagaaac gatatgtcga tgcgaaaaaa gttcaagaac atcatgcaat
    43081 tattccgaca aagacgattc cgactcctgc acgctttgcg aagctttcca aattacagca
    43141 agcaatctat ttgcaagttt tgcgcactac tgtggctatg tttgctgcag attatatcta
    43201 cgaagagacg accgtaatga cgggcgtaca gcaattaaag ctaaaaagca tcggaaagat
    43261 cccattacag caaggttggc aggaaatctt acgcatcaag tcaaaaaaga aagaagctca
    43321 gactcttcct cctatcgtag aaaatgaaga agcagaagta gaactgaaaa gtatggagaa
    43381 agaaacacaa cctccaaaac cctataccga aggaacgttg atcacagcaa tgaaaactgc
    43441 gggaaaaacg ctggataatg atgaagcgca agaaattctc aaagaagtag aaggaatagg
    43501 gacagaagct acacgagcca atatcattga aaatcttaag cagaagcatt acatcgaagt
    43561 caataaaaac gaaataactg ttacgccaaa aggaatcact ctttgtaagg cagtagccaa
    43621 tgaaccatta ctgacgagcg cagaaatgac tgcgcgctgg gaagggtact tgagaaaaat
    43681 tggtaaacaa gaggggacac catctgtttt tttagagaat atcaaaaaat tcatcctaca
    43741 tttattggca gaagttccta atcaaatcga caaggtagac ttttcagatg agattagcgg
    43801 gacacgtcag ataaaagcga tggagaagaa aaacgatcaa ttaggaagat gtccaaaatg
    43861 taaaaaggga atagtcatgc tttatcctaa aatcgctaca tgcttgaatc cggaatgcga
    43921 cttcaaattg tggccaacag tagctaaaaa gaagctgact aaaacgaatc tcagagattt
    43981 gctttcaaaa ggaaagacaa gtaaagttgt caaaggtttt actggtaaaa aagggaaatt
    44041 cgatgcagtt ttagtattaa aagaagatat gacaatcggt ttttcttttc catggatcga
    44101 aaacagtgga acaaaagcag ttgatggaaa caaagaaaca tcaagaaaaa ataatgaaaa
    44161 cgaagtagag taaatgaaaa caatgaaata gcggaatatt tattccgaaa agtgagaata
    44221 taaataccaa taaaactaaa aaatatcagt gaatcaagca aaaacgttgg ggtaggctga
    44281 ttttatatag atagcaagga gtttttttat ttgaaaaaag tgcattcatt tttaaattat
    44341 cttatgggag caatcacgat tgtggctgtg taccaagcat ttctaaagta tcctgattac
    44401 gggacgaagt tgttgtcatt gacgcacaat caaacagcat attcaccaac gttgatttta
    44461 tcgactttga ctgcaggagc taaaagcgct acttattttt ttcataaaac gacaagcatg
    44521 ctaaagcgaa agaaacagcg aaaacaattg ttggtggtgg tagcggctat gatcattatt
    44581 tattttctac cagttattac acaagtattt catcaatatt ttggttaaga atacgagagt
    44641 ttgtgacaaa ag
//
LOCUS       DASJPH010000009        43023 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJPH010000009 DASJPH010000000
VERSION     DASJPH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.009"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            242..526
                     /locus_tag="U3995_000484"
     CDS             242..526
                     /locus_tag="U3995_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1795833.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(807..1226)
                     /locus_tag="U3995_000485"
     CDS             complement(807..1226)
                     /locus_tag="U3995_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1795834.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1228..1812)
                     /locus_tag="U3995_000486"
     CDS             complement(1228..1812)
                     /locus_tag="U3995_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1795835.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1859..3334)
                     /locus_tag="U3995_000487"
     CDS             complement(1859..3334)
                     /locus_tag="U3995_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1795836.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3515..3826
                     /locus_tag="U3995_000488"
     CDS             3515..3826
                     /locus_tag="U3995_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795837.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3941..6277)
                     /locus_tag="U3995_000489"
     CDS             complement(3941..6277)
                     /locus_tag="U3995_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1795838.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6419..7087
                     /locus_tag="U3995_000490"
     CDS             6419..7087
                     /locus_tag="U3995_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1795839.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7090..8133
                     /locus_tag="U3995_000491"
     CDS             7090..8133
                     /locus_tag="U3995_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1795840.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8370..9251)
                     /locus_tag="U3995_000492"
     CDS             complement(8370..9251)
                     /locus_tag="U3995_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1795841.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9410..10138
                     /locus_tag="U3995_000493"
     CDS             9410..10138
                     /locus_tag="U3995_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1795842.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10294..10611
                     /locus_tag="U3995_000494"
     CDS             10294..10611
                     /locus_tag="U3995_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1795843.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10604..11212
                     /locus_tag="U3995_000495"
     CDS             10604..11212
                     /locus_tag="U3995_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1795844.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11267..12058
                     /locus_tag="U3995_000496"
     CDS             11267..12058
                     /locus_tag="U3995_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1795845.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12196..12684
                     /locus_tag="U3995_000497"
     CDS             12196..12684
                     /locus_tag="U3995_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1795846.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12735..13607
                     /locus_tag="U3995_000498"
     CDS             12735..13607
                     /locus_tag="U3995_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1795847.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13783..14145
                     /locus_tag="U3995_000499"
     CDS             13783..14145
                     /locus_tag="U3995_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1795848.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14154..14906
                     /locus_tag="U3995_000500"
     CDS             14154..14906
                     /locus_tag="U3995_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1795849.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14920..16722
                     /locus_tag="U3995_000501"
     CDS             14920..16722
                     /locus_tag="U3995_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795850.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16999..18039
                     /locus_tag="U3995_000502"
     CDS             16999..18039
                     /locus_tag="U3995_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1795851.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18091..20235)
                     /locus_tag="U3995_000503"
     CDS             complement(18091..20235)
                     /locus_tag="U3995_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1795852.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20592..21965)
                     /locus_tag="U3995_000504"
     CDS             complement(20592..21965)
                     /locus_tag="U3995_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1795853.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21965..22360)
                     /gene="rbsD"
                     /locus_tag="U3995_000505"
     CDS             complement(21965..22360)
                     /gene="rbsD"
                     /locus_tag="U3995_000505"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1795854.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22388..23305)
                     /gene="rbsK"
                     /locus_tag="U3995_000506"
     CDS             complement(22388..23305)
                     /gene="rbsK"
                     /locus_tag="U3995_000506"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1795855.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23413..24426)
                     /locus_tag="U3995_000507"
     CDS             complement(23413..24426)
                     /locus_tag="U3995_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1795856.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24507..25106)
                     /locus_tag="U3995_000508"
     CDS             complement(24507..25106)
                     /locus_tag="U3995_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1795857.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25292..25993
                     /locus_tag="U3995_000509"
     CDS             25292..25993
                     /locus_tag="U3995_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1795858.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            26003..28714
                     /locus_tag="U3995_000510"
     CDS             26003..28714
                     /locus_tag="U3995_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795859.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29165..30793
                     /gene="treC"
                     /locus_tag="U3995_000511"
     CDS             29165..30793
                     /gene="treC"
                     /locus_tag="U3995_000511"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1795860.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30853..32034)
                     /locus_tag="U3995_000512"
     CDS             complement(30853..32034)
                     /locus_tag="U3995_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1795861.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32286..33809
                     /locus_tag="U3995_000513"
     CDS             32286..33809
                     /locus_tag="U3995_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1795862.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33878..34606)
                     /locus_tag="U3995_000514"
     CDS             complement(33878..34606)
                     /locus_tag="U3995_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1795863.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34619..35899)
                     /locus_tag="U3995_000515"
     CDS             complement(34619..35899)
                     /locus_tag="U3995_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1795864.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36040..36642)
                     /locus_tag="U3995_000516"
     CDS             complement(36040..36642)
                     /locus_tag="U3995_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1795865.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36887..37093
                     /locus_tag="U3995_000517"
     CDS             36887..37093
                     /locus_tag="U3995_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795866.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37405..38124
                     /locus_tag="U3995_000518"
     CDS             37405..38124
                     /locus_tag="U3995_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1795867.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38205..38888)
                     /locus_tag="U3995_000519"
     CDS             complement(38205..38888)
                     /locus_tag="U3995_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1795868.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39106..41292
                     /locus_tag="U3995_000520"
     CDS             39106..41292
                     /locus_tag="U3995_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1795869.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41638..42048
                     /gene="spx"
                     /locus_tag="U3995_000521"
     CDS             41638..42048
                     /gene="spx"
                     /locus_tag="U3995_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1795870.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42062..42418
                     /locus_tag="U3995_000522"
     CDS             42062..42418
                     /locus_tag="U3995_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1795871.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        14030 a         7568 c         8601 g        12824 t
ORIGIN      
        1 gtagatgcaa gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct
       61 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt
      121 gaccgaggag ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt
      181 tgtcacagtt ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa
      241 aatgagggaa atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg
      301 ggcatatatt atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt
      361 ccaagcaact tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa
      421 tccagatggt tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg
      481 aaaagaaatc ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca
      541 aaagacgtgt cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt
      601 caaatttatg caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg
      661 aagcggcttt atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag
      721 ctcaaaaacg gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca
      781 taacgctgat ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact
      841 ggttctttga tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct
      901 tctgacatcg gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg
      961 gaagtgatcg tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct
     1021 aagacatcgt ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct
     1081 gtatcacgag aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg
     1141 atcaataagt ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt
     1201 acttcaacta tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt
     1261 tacaatttca aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt
     1321 ttgaggctga ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa
     1381 tttagtcaag ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa
     1441 ctttcgttgc tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc
     1501 aacagagata cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa
     1561 aaatgatccg ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag
     1621 gctttcgaat tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg
     1681 actgatgatt ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat
     1741 ggcataaatg gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc
     1801 caattgttcc attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt
     1861 atttcaaata tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt
     1921 tcatatcgaa aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa
     1981 tagagccagg attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg
     2041 ggatcacgat gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt
     2101 tcatgtcgaa aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg
     2161 ctgataaagc aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac
     2221 ggatttgcat gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta
     2281 cttttcctgg aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg
     2341 ccatagatac gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc
     2401 ggtgagtaac tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc
     2461 caggaaattc tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat
     2521 ttggcatgat ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg
     2581 ctataccatc aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat
     2641 tgtagccaag tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta
     2701 aaccaatatt gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt
     2761 gaccaatgtg gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa
     2821 ccatacgatg gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac
     2881 ccattgtgat gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc
     2941 gaatatcgaa gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt
     3001 tgtctacttt cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg
     3061 ttcgataatc atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt
     3121 tcacacgtgt gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag
     3181 tcggacgttt tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt
     3241 caggatcgat ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga
     3301 tgacttttct taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta
     3361 cctatctata gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc
     3421 tacaatggaa tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa
     3481 aagaaagcgc atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc
     3541 taaagcagta aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga
     3601 agtaaaatac gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa
     3661 aaaaggaaga ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac
     3721 tgaagtaacg attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc
     3781 agaagagttg tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa
     3841 aaacagcgcg aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca
     3901 gcgctcttat gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga
     3961 tagcggcgtc ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata
     4021 tagtagagac gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta
     4081 gatccgtcat gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca
     4141 cacctaatat cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga
     4201 tgtctttttt acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca
     4261 cagatgtata agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa
     4321 aagcaataag gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc
     4381 cagctaaatc agtataaatg attttatctt catctgattt gcctttattg taatcatcta
     4441 gataatccaa gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag
     4501 gcaagctgtc tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt
     4561 cagtagttaa gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt
     4621 tttcgttgac gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat
     4681 tcattgtaga agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat
     4741 tctgatacat cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat
     4801 agtaagtatt gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt
     4861 tttcgtcttc tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat
     4921 tgccgtctac gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag
     4981 aataattgtc ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca
     5041 ctcccacacc tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat
     5101 tttgattcga gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc
     5161 ctgtagtacg ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat
     5221 cgacgtatgt ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg
     5281 cagtaatcgt tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac
     5341 gagtctggtt agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt
     5401 gcgtgttatc gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga
     5461 tgatcccaat actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat
     5521 tgttgaagga aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt
     5581 gatcatcttt tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa
     5641 attcaatgat tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca
     5701 ctaatttttc tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat
     5761 ctaacgctcc ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag
     5821 cgatggccac tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat
     5881 ttgtcaagcc gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa
     5941 caccacttaa tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt
     6001 tgtaactttg aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct
     6061 taaagtcttt agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc
     6121 caccaatcac attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga
     6181 attctttttg tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga
     6241 ctttataata tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa
     6301 atattcggta tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta
     6361 actgaagatg aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat
     6421 gaatcgtatt ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt
     6481 ggaacgagtg ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact
     6541 agaagcgaca cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta
     6601 tgacttgcta gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc
     6661 aaaggccgat tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac
     6721 aaaacccatc gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa
     6781 gatcaattac agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac
     6841 agtgttacaa gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa
     6901 attactgtct tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg
     6961 attagatacg aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg
     7021 ttttgaaaag acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt
     7081 tatttaaaga tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc
     7141 ttcatttcga tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga
     7201 cattctatct cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt
     7261 gcggggttca gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg
     7321 catccaatca gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt
     7381 cgaatggatg aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt
     7441 atggtccaag aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc
     7501 catgaattca aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca
     7561 aatctctctg acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag
     7621 ctttcacaat taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa
     7681 ctggaaaaga aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag
     7741 cctaaatggg agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca
     7801 ggcaatgaag agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac
     7861 aatcaaatca atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg
     7921 gaagtgaccg attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa
     7981 ttttatcaag gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt
     8041 aaaaaaattc tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga
     8101 acgacagcaa tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc
     8161 tattgaaggt ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg
     8221 ttcttttggc ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca
     8281 ttgaaacaag gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg
     8341 ggtggataga tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa
     8401 aatgtatctg aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct
     8461 gttgttggag aaatagggag aatcacagta ttttctttta atagattctt gactgttttt
     8521 tccataacaa tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt
     8581 gctcctttat aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt
     8641 tgatacaagt tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga
     8701 tccaatttct ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct
     8761 gtttcagtag gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg
     8821 atcgtgtcac tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc
     8881 aactggactg taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta
     8941 taattgatca ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag
     9001 gtttcaagca cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc
     9061 aataagcttc cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg
     9121 tctaattctg tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt
     9181 cgatcagcag ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta
     9241 tggatatcca tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg
     9301 aattggtgta atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag
     9361 aaatttttct gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg
     9421 agttttgatc aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa
     9481 taaattaagt gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg
     9541 gatgggaatc tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt
     9601 gataggaaca gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc
     9661 gctgatgcaa gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga
     9721 tgaatttatc agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat
     9781 ctatggaagt ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt
     9841 ccttgatttt ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat
     9901 agcaattccc caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc
     9961 attgactaca ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc
    10021 taccggtttt agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat
    10081 gatcgttatc atgccactga gctggatgtg gacaaattgg attatgccac tgttataata
    10141 aaaatcaaag agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt
    10201 tttgtatttg acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat
    10261 aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt
    10321 cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca
    10381 aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga
    10441 ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga
    10501 tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt
    10561 gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa
    10621 tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta
    10681 ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg atgaatatgg
    10741 tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc
    10801 agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat
    10861 cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg
    10921 gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat
    10981 cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct
    11041 gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc
    11101 gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg
    11161 gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac
    11221 acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg
    11281 cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg
    11341 tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt
    11401 catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac
    11461 gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa
    11521 tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac
    11581 tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc
    11641 aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt
    11701 atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg
    11761 attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct
    11821 agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt
    11881 gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa
    11941 agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac
    12001 tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc
    12061 ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt
    12121 ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac
    12181 agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg
    12241 gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa
    12301 aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat
    12361 gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt
    12421 atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt
    12481 cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt
    12541 acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg
    12601 acacaggcaa gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg
    12661 gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga
    12721 gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg
    12781 tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag
    12841 cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa
    12901 gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt
    12961 taggtacgat tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg
    13021 tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa
    13081 accccatcga aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat
    13141 tcgatgtagc gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata
    13201 tcgctagcca atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca
    13261 cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg
    13321 tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca
    13381 tgctccaacg tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag
    13441 ctgtcacaat cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca
    13501 ccgatgatcg gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag
    13561 gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta
    13621 agataggaag aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta
    13681 aagaaaatga caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt
    13741 ttttctataa tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc
    13801 ggtctacttg ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt
    13861 atgggtggag aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa
    13921 tcagactctg gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag
    13981 ggtaaggaaa aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca
    14041 tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa
    14101 aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca
    14161 aaatagtcga agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc
    14221 aagcattaag cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg
    14281 cttctggttc tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag
    14341 atgggcatat ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag
    14401 attttcgtgc gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga
    14461 cagcacaaga aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca
    14521 agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc
    14581 cttctgagat ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac
    14641 aaccaacgat tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg
    14701 atcttttaga tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga
    14761 cgcatgatcc gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta
    14821 tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat
    14881 ctattcttgg aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt
    14941 actgggatca agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca
    15001 tctgctattt tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat
    15061 acgatcgttg cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc
    15121 acattcgttt atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat
    15181 gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa
    15241 acattcttag tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca
    15301 agtatcgtcc accgattatt agtggatcag ttagatctca atatttcgca ttttacacct
    15361 tttagtattc acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct
    15421 gctgttgtca atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat
    15481 cagacaccaa caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga
    15541 attgcttttc taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt
    15601 ggtatagggg tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg
    15661 atcattttct ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac
    15721 aatttcacat tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg
    15781 gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat
    15841 gaaattgcag acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg
    15901 gcagaagatc agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa
    15961 aaagaagatg cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc
    16021 atgataaaac atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa
    16081 aaaatgaatg aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa
    16141 gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc
    16201 actttacagc tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt
    16261 gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa
    16321 tatgaaatgt atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc
    16381 ctaggtttag ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga
    16441 agcaagagtg acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg
    16501 cttaaacaat cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt
    16561 gtagtccatg tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa
    16621 gatatttggg ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg
    16681 acatggttat atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca
    16741 agtaataaga acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt
    16801 accgcagatg caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt
    16861 tgtcacaacc tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc
    16921 acggaataag ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga
    16981 aaagagggtg caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg
    17041 attacgatca cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt
    17101 gttcattttc aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa
    17161 gcaaaagaat atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga
    17221 cggattacga atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg
    17281 gacactggtc attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat
    17341 acagtcatca atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg
    17401 gaaaacggat ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat
    17461 acatggcgag tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat
    17521 catgtctatt tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg
    17581 ttgaatagcc gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt
    17641 tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc
    17701 agtgattttc gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa
    17761 caatctggtt tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa
    17821 gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg
    17881 gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg
    17941 ccaggagcgg agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc
    18001 gagtttgtaa cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt
    18061 gacagtgtca catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa
    18121 tgatttgtct aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt
    18181 agttgtttct ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac
    18241 acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat
    18301 tggcacgcca tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat
    18361 ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag
    18421 gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg
    18481 tccttccaaa tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt
    18541 gtagaaatca ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact
    18601 ttccttgtaa gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact
    18661 atcttgttca agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa
    18721 aaggttatga tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc
    18781 ttgagccatc atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc
    18841 tgcaatcttt ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc
    18901 taatccatct aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc
    18961 taagtttact cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt
    19021 atctcctggt tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg
    19081 agtaacaccg gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg
    19141 aatctgcgta cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc
    19201 tttcatatat acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc
    19261 ccatacatat ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt
    19321 cgttgtattt gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc
    19381 agaagatgga ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt
    19441 cgttccgcca ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt
    19501 gttattcgat ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt
    19561 attattgttt gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt
    19621 tggcgtttcc ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt
    19681 tgaagtatcc ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt
    19741 aaaggtcttt gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg
    19801 ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac
    19861 cgttccactt tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt
    19921 cagttggatc ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt
    19981 ttcattttct gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga
    20041 gttagttgtt ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct
    20101 ggatgtagtc gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac
    20161 ggtattggtc gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt
    20221 ttttttattt ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag
    20281 cattcattag acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa
    20341 tctgtctgtg tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt
    20401 tctttgacgt tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata
    20461 aaaatggtta tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa
    20521 aaataatgat cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc
    20581 tttttcatta actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg
    20641 atcattgcta cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa
    20701 atgaatgcag gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca
    20761 aatcccatta cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc
    20821 aatgggatcg caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca
    20881 aaagcagaac cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa
    20941 gctgcaccag ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca
    21001 taagcagaga tattactgat catgacaggg tttacagccc attggatacc aaatattacc
    21061 aaaatattcc aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc
    21121 caagtaattg cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt
    21181 aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt
    21241 tcgcgaatat attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc
    21301 gccggcatga cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta
    21361 gctgctttgc cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca
    21421 atgacagccg caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag
    21481 attggcagaa aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt
    21541 gtttcactaa gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa
    21601 ccggcaaaca gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg
    21661 atcggattta gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa
    21721 gcaggagcca atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac
    21781 ttaatggaag ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat
    21841 ttgatacgaa gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac
    21901 tgttcaatca gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt
    21961 tccattagaa gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg
    22021 tacgaataaa tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc
    22081 taatcggtgt atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct
    22141 ttatttcttc cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa
    22201 cagataaaaa acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg
    22261 ggacaggcat gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta
    22321 ctcgtgaaat atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt
    22381 gttaatttta gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac
    22441 cataagactg cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt
    22501 tactaaggtc tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg
    22561 ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat
    22621 agaaagcgcc ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt
    22681 tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg
    22741 tttctgtttc atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt
    22801 cacgagctgg tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag
    22861 ctaggatcgt cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa
    22921 tcagttgctt gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga
    22981 tcaagatact attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc
    23041 cttggatttc agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact
    23101 gttgtccttc gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt
    23161 tggctgccac tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat
    23221 gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac
    23281 ttccaattac tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg
    23341 ggaaaattat tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc
    23401 acaccttgaa agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa
    23461 tcttttttct tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga
    23521 tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc
    23581 gcacatatct atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc
    23641 ttcctctaat ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt
    23701 tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt
    23761 aggtaagagg atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc
    23821 ctcaatccga gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg
    23881 atgtcctaga ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg
    23941 tacggcatca ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg
    24001 ttttttcaaa ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc
    24061 atccacacct cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc
    24121 agcattacat aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat
    24181 cagtgtactg aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc
    24241 cttggtaatc atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc
    24301 taaaactttc tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga
    24361 gacagttgtg attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt
    24421 caccatgttc atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag
    24481 taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt
    24541 ttcgttgagc gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat
    24601 tctttgtcac gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag
    24661 gcatctaatg cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt
    24721 tcgattccac gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg
    24781 tattttgtga tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca
    24841 gtgaattctg gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa
    24901 gggaaagtgg agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt
    24961 cttactaaca aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg
    25021 ttatttacga tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa
    25081 atcatatttt cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat
    25141 gtaagtaaat cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct
    25201 agagaaattc agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag
    25261 cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata
    25321 caaacgatac aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat
    25381 tgaaaaaggc gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct
    25441 taatatttta ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga
    25501 tattgcgcaa ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt
    25561 tttccaattc tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac
    25621 ggaagtttct cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca
    25681 tcgattgaat aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc
    25741 tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga
    25801 ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa
    25861 taccgttttg atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca
    25921 cattaatgat gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga
    25981 gattgtatgg tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga
    26041 aattcggcaa tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact
    26101 tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt
    26161 tggtacagca ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga
    26221 tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta
    26281 ttccaatact ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca
    26341 gaacaagcta caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga
    26401 tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga
    26461 attgaaagaa aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa
    26521 tttggagaga ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc
    26581 tgaatccgtt tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa
    26641 gaatttgaat acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc
    26701 agaaaagtat ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa
    26761 cgaagaatta gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc
    26821 aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca
    26881 gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact
    26941 tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga
    27001 attgaatcga aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga
    27061 acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat
    27121 tgccaatgtt tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat
    27181 gacacgtatg gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac
    27241 gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat
    27301 tgttttagga acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa
    27361 cgagcggtat gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc
    27421 agctgccgca ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt
    27481 aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt
    27541 tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg
    27601 aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc
    27661 cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca
    27721 atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag
    27781 tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat
    27841 tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat
    27901 cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa
    27961 cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga
    28021 cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga
    28081 agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta
    28141 tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat
    28201 gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa
    28261 tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc
    28321 ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct
    28381 aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt
    28441 ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg
    28501 aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc
    28561 tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg
    28621 attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat
    28681 cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt
    28741 tgacctgagt attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga
    28801 gctaataccg aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc
    28861 tgcatcgagc aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag
    28921 actgattgcc gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg
    28981 ctattttaaa caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac
    29041 agacctctta gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat
    29101 aaatgataac ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat
    29161 atccatgaca tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag
    29221 taatggtgat ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga
    29281 tctaggcatc gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg
    29341 ttatgatatc tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga
    29401 acagctaata gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa
    29461 tcacgtttcg acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca
    29521 ggacttcttc attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc
    29581 ttggtcgcca tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc
    29641 tgacttgaac tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg
    29701 gatggaaaaa ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga
    29761 acaattgaaa aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac
    29821 ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac
    29881 agtcggtgaa atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag
    29941 agaagaatta tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa
    30001 taaatggtct actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg
    30061 agaaaagatg agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc
    30121 acgtgcgctc aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct
    30181 agcaaccatg atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg
    30241 catgatcgac ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc
    30301 ttataagtta ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc
    30361 aaaatctcgc gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt
    30421 tactacaggg aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa
    30481 agaactatct tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa
    30541 aacatatcca atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt
    30601 ttatggcttt cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga
    30661 taaagataca aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa
    30721 ttattccgat actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt
    30781 gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg
    30841 acgttttgtg ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc
    30901 tttatcgcca taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag
    30961 taatttgact aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc
    31021 acccatgaca agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat
    31081 tactcgagga atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa
    31141 tttgacagcc ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat
    31201 aaacggccca atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc
    31261 aaattctaat ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc
    31321 aatgatttgc ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt
    31381 aaaatctgta cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg
    31441 atctctcaaa aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt
    31501 aacagttgaa aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa
    31561 tccagctata tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc
    31621 aaaaggtgtt ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg
    31681 caaactactg atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc
    31741 aagccccaat aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag
    31801 gtgcttgctc aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa
    31861 taatgggggc aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg
    31921 atggaaaaaa taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt
    31981 ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt
    32041 tcctccttta tctataaatt cattatagac aaaagcgggt ataaaaggaa aatgatagag
    32101 gttgattgca aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa
    32161 aacgcttgac agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta
    32221 ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt
    32281 cataaatgaa acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg
    32341 cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct
    32401 tttatcccat gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat
    32461 tgattccagc agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga
    32521 tccaagatac agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa
    32581 tccaaaaaac attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt
    32641 tacttccggg aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata
    32701 cacatgagct agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa
    32761 acatacgtga tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg
    32821 cagcagccta taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc
    32881 aagcaaaaca aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat
    32941 atggattgaa tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga
    33001 gaattgctga actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag
    33061 aaaacgccaa agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag
    33121 tattgaatcc tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt
    33181 ctatcatgga agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag
    33241 aagatacttt gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg
    33301 atgtacaaga tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg
    33361 tagaagatgg gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga
    33421 cagcacaaga atataaagaa tattatacga agggctatca aacagatatt gcttcaatca
    33481 agatcagtga gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt
    33541 atcgttatgt agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc
    33601 tattcgaagc agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca
    33661 cgattgcacc ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag
    33721 tattgctgaa agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg
    33781 aaattgcaca ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg
    33841 gctggtcatt tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc
    33901 cacggataga ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat
    33961 taatcgaccg cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc
    34021 gatgcatttt ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat
    34081 ggacttccag tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg
    34141 gtaaaaattt gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca
    34201 tttgaaaatc ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa
    34261 gatcttcttt gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca
    34321 tttccgtatg ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt
    34381 taccgatcgc tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac
    34441 caaaagaaga agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa
    34501 tatggacata actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa
    34561 cttctaattg ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt
    34621 attttatttg tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga
    34681 aaagataagg attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt
    34741 ttggatttgt ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg
    34801 ttgttgtatt gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga
    34861 tttttttctt ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca
    34921 tacgtacaag gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct
    34981 ggacgatcaa ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct
    35041 caggaacttt catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc
    35101 gtgtaggtaa tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa
    35161 tatagctgtg aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt
    35221 gatagttttt ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata
    35281 actgatcagc taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa
    35341 cacacagata tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag
    35401 aaaaacgggt ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac
    35461 cgatcatacg gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag
    35521 ctaataaagc agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg
    35581 gttcaataac cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta
    35641 aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa
    35701 agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa
    35761 tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt
    35821 tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg
    35881 ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa
    35941 ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag
    36001 cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg
    36061 aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa
    36121 aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag
    36181 agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc
    36241 aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat
    36301 taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg
    36361 aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa
    36421 tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca
    36481 tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa
    36541 aacttctaag acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct
    36601 gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc
    36661 acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac
    36721 atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa
    36781 gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt
    36841 atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca
    36901 attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa
    36961 aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt
    37021 agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac
    37081 aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt
    37141 tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc
    37201 cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga
    37261 cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt
    37321 agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga
    37381 agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc
    37441 ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt
    37501 tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat
    37561 acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc
    37621 agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg
    37681 aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa
    37741 acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat
    37801 attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa
    37861 cagctagaac aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa
    37921 gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat
    37981 ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag
    38041 aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca
    38101 aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt
    38161 tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga
    38221 ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa
    38281 atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata
    38341 gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct
    38401 ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt
    38461 gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat
    38521 gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta
    38581 acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct
    38641 ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg
    38701 gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat
    38761 agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata agtataactg
    38821 tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa
    38881 gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac
    38941 gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt
    39001 ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg
    39061 aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa
    39121 attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa
    39181 gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc
    39241 ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa
    39301 atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat
    39361 gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc
    39421 tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg
    39481 atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag
    39541 agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa
    39601 atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat
    39661 actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata
    39721 tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac
    39781 ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat
    39841 gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa
    39901 agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag
    39961 aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt
    40021 caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc
    40081 aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca
    40141 gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat
    40201 tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat
    40261 gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat
    40321 ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt
    40381 caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag
    40441 cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca
    40501 tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg
    40561 caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg
    40621 atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa
    40681 aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca
    40741 gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg
    40801 cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa
    40861 gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg
    40921 caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga
    40981 aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat
    41041 gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa
    41101 gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga
    41161 ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg
    41221 aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa
    41281 aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg
    41341 aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta
    41401 tttctggaac acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc
    41461 ttatttattt tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc
    41521 attattcaaa cgtaaataat cagttatttt gatttttttt atattttatt agctaataat
    41581 tggtatttta aaagaaatta ctgtagaatt ggattataat taaaaaggag tgagaaaatg
    41641 ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa
    41701 gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag
    41761 atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg
    41821 aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc
    41881 ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata
    41941 ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt
    42001 gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt
    42061 cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg
    42121 gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac
    42181 agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac
    42241 ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact
    42301 gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga
    42361 agttctattt attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac
    42421 tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag
    42481 catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca
    42541 atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg
    42601 tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta
    42661 ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata agataactga
    42721 gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt
    42781 cttgacaaat aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg
    42841 aaatcaaaaa aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa
    42901 agttgctacg gcagccaaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa
    42961 actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa
    43021 aca
//
LOCUS       DASJPH010000010        42608 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJPH010000010 DASJPH010000000
VERSION     DASJPH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.010"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..995)
                     /locus_tag="U3995_000523"
     CDS             complement(39..995)
                     /locus_tag="U3995_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1795872.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1169..1729
                     /locus_tag="U3995_000524"
     CDS             1169..1729
                     /locus_tag="U3995_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795873.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1722..2546
                     /locus_tag="U3995_000525"
     CDS             1722..2546
                     /locus_tag="U3995_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1795874.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2556..3173
                     /locus_tag="U3995_000526"
     CDS             2556..3173
                     /locus_tag="U3995_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1795875.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3191..3874
                     /locus_tag="U3995_000527"
     CDS             3191..3874
                     /locus_tag="U3995_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1795876.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4557..5840
                     /locus_tag="U3995_000528"
     CDS             4557..5840
                     /locus_tag="U3995_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1795877.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5856..6743
                     /locus_tag="U3995_000529"
     CDS             5856..6743
                     /locus_tag="U3995_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1795878.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6760..7596
                     /locus_tag="U3995_000530"
     CDS             6760..7596
                     /locus_tag="U3995_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1795879.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7599..8222
                     /locus_tag="U3995_000531"
     CDS             7599..8222
                     /locus_tag="U3995_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795880.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8219..9511
                     /locus_tag="U3995_000532"
     CDS             8219..9511
                     /locus_tag="U3995_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1795881.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9870..9967
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10083..10664
                     /locus_tag="U3995_000533"
     CDS             10083..10664
                     /locus_tag="U3995_000533"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1795882.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10667..11974
                     /locus_tag="U3995_000534"
     CDS             10667..11974
                     /locus_tag="U3995_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1795883.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12390..12878
                     /gene="purE"
                     /locus_tag="U3995_000535"
     CDS             12390..12878
                     /gene="purE"
                     /locus_tag="U3995_000535"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1795884.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12871..13995
                     /gene="purK"
                     /locus_tag="U3995_000536"
     CDS             12871..13995
                     /gene="purK"
                     /locus_tag="U3995_000536"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1795885.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            14008..15303
                     /gene="purB"
                     /locus_tag="U3995_000537"
     CDS             14008..15303
                     /gene="purB"
                     /locus_tag="U3995_000537"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1795886.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15425..16813)
                     /gene="ltrA"
                     /locus_tag="U3995_000538"
     CDS             complement(15425..16813)
                     /gene="ltrA"
                     /locus_tag="U3995_000538"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1795887.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(17382..17759)
                     /locus_tag="U3995_000539"
     CDS             complement(17382..17759)
                     /locus_tag="U3995_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795888.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            18015..18743
                     /locus_tag="U3995_000540"
     CDS             18015..18743
                     /locus_tag="U3995_000540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1795889.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            18743..18997
                     /gene="purS"
                     /locus_tag="U3995_000541"
     CDS             18743..18997
                     /gene="purS"
                     /locus_tag="U3995_000541"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1795890.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            18999..19670
                     /gene="purQ"
                     /locus_tag="U3995_000542"
     CDS             18999..19670
                     /gene="purQ"
                     /locus_tag="U3995_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1795891.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            19671..21893
                     /gene="purL"
                     /locus_tag="U3995_000543"
     CDS             19671..21893
                     /gene="purL"
                     /locus_tag="U3995_000543"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1795892.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            21878..23317
                     /locus_tag="U3995_000544"
     CDS             21878..23317
                     /locus_tag="U3995_000544"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1795893.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            23340..24392
                     /locus_tag="U3995_000545"
     CDS             23340..24392
                     /locus_tag="U3995_000545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1795894.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            24389..24967
                     /gene="purN"
                     /locus_tag="U3995_000546"
     CDS             24389..24967
                     /gene="purN"
                     /locus_tag="U3995_000546"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1795895.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            24964..26499
                     /gene="purH"
                     /locus_tag="U3995_000547"
     CDS             24964..26499
                     /gene="purH"
                     /locus_tag="U3995_000547"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1795896.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            26515..27789
                     /gene="purD"
                     /locus_tag="U3995_000548"
     CDS             26515..27789
                     /gene="purD"
                     /locus_tag="U3995_000548"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1795897.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            27911..28825
                     /locus_tag="U3995_000549"
     CDS             27911..28825
                     /locus_tag="U3995_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1795898.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(28958..30523)
                     /locus_tag="U3995_000550"
     CDS             complement(28958..30523)
                     /locus_tag="U3995_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1795899.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(30516..31967)
                     /locus_tag="U3995_000551"
     CDS             complement(30516..31967)
                     /locus_tag="U3995_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1795900.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(31981..32997)
                     /locus_tag="U3995_000552"
     CDS             complement(31981..32997)
                     /locus_tag="U3995_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1795901.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            33217..34305
                     /locus_tag="U3995_000553"
     CDS             33217..34305
                     /locus_tag="U3995_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1795902.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            34359..35885
                     /locus_tag="U3995_000554"
     CDS             34359..35885
                     /locus_tag="U3995_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1795903.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            35887..37092
                     /locus_tag="U3995_000555"
     CDS             35887..37092
                     /locus_tag="U3995_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1795904.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            37349..38326
                     /gene="guaC"
                     /locus_tag="U3995_000556"
     CDS             37349..38326
                     /gene="guaC"
                     /locus_tag="U3995_000556"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1795905.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            38590..40014
                     /locus_tag="U3995_000557"
     CDS             38590..40014
                     /locus_tag="U3995_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1795906.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            40475..41584
                     /gene="pdhA"
                     /locus_tag="U3995_000558"
     CDS             40475..41584
                     /gene="pdhA"
                     /locus_tag="U3995_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1795907.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            41587..42564
                     /locus_tag="U3995_000559"
     CDS             41587..42564
                     /locus_tag="U3995_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1795908.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        13424 a         7403 c         9472 g        12309 t
ORIGIN      
        1 tacttgatgc agaacagctt tttcacaacc ttttaaaatt acatattttt ccatagaact
       61 tgcatttgat ccaagatttc gatgacatct tttgtcaaga agagagagcg attgggcaag
      121 tcaccttcaa tcatgtgtac catattcttt atttcgtaat tcattgcttc agatgtattc
      181 ccgctttcga tgaattcctt tgtaccatct gtaaataaga tctccgcacg atcagcacgc
      241 gggtaatcag caatcgtcac ataaccgttc tcaaaggcaa cgataccgac ttttggcatt
      301 tttgcctgaa aagtcaagct gacagcagca agttcatttt ctttgttacg caagatagtg
      361 acggattgtt cgtccacgcc tgtttcaaaa ggaaccattg tagaggcaac tacttcaggt
      421 tgagagctca aaaagaaacg agcgaaagat acagcatacg taccgatatc caacagagct
      481 cctccagcta attctggatt gaagaaccgg tttttagggt ctggttcttt gtaactgccg
      541 aaaggtgctt ggatcatttt taaagccccc agttttcctg tatccattat tgagcgtaac
      601 tgctgataaa gtggcatgtt gaagattgtc atcgcttctg ctaagatcaa attcttttct
      661 tctgctaagc gcatagcgtc agccagttct tttttattca tcgtgatggc tttttcacat
      721 agaacgtgtt tgcctgcttc caatgctgcc aagatatggt cgatatgctg tctatttggt
      781 acagcaatgt acacgatgtc gatttcagca tcgtctaaca tttcttgata gcttccgtaa
      841 gcttttggaa tattgtaacg gtgagcgaag ttttccgctt tttctgaagt tctggaagca
      901 actgctgtta gttcgctcgt ctcttgattg aagctttcgg caaactcatg tgcgatggta
      961 ccaagaccaa cgattcccca ttgatatttt ttcatctgaa aacgctcctt catagttgga
     1021 ttgagtttct tgtctgtcta ttattataac aaaaatcacg aagaataaag aggagaggac
     1081 ttgaaacatt aaaaaaaatg aaagcagcga tttaagaggt gctttttgat caaaacttaa
     1141 gtagaatagt taaaaggagg ggataaacgt gcatcaagta tatattgatc ttattatgag
     1201 tgtattatta gaacagtttg aaactgaaaa aaatttttta gatgaatact tgctgatcag
     1261 tccagaacaa tggcaaaaat ggaaagatgg cgagaaaaat ctgccatctg aagcgatgca
     1321 gaaagtaaaa aatcttttca ctgattatga atggatgcta acgcaaaaga ttttgcgtca
     1381 aacgattatt tttcccgaaa aacgaaacgt tgccgttgca gagtacaagc aaatcaaaac
     1441 acgcatcgcg caaaaatggc ttagcagcaa agcagcaaaa gtcgagctga tccctttgaa
     1501 aaataatcag caggctggta gataccttga cttgaaagta agtattcaat atgatgaatg
     1561 gggctatgat gatattttga attttcgtct gccagcagat gttcaaaaac agatcgaagg
     1621 agaaaaaatc gaacttctcg attgggtgaa tgaaaatctg gaagaaactt acgttcatga
     1681 ggcgaatggg gaacgtgaag aacaacgaaa ggatttttca agtgaataaa tttttaggat
     1741 ttggagcagt tgtgattatg atcgcagcaa tgggctatgt tatttttagc gatgatgatt
     1801 cgcatgttca agccggcaat caagcagtcg aacgaacaag caccaaaaca actgctagta
     1861 aagaaaagaa cagtcaaaca accagcagca aggtaaagga caaagaaaca aaagatcgta
     1921 cgtccaaaca attgccagat gttaaaacag acgattggaa gctagttttg gttggtccag
     1981 atcacaaatt agaaaaagaa attgatgagt ctactcaatt ggcatcatta agtaacggat
     2041 acttgatcga caaacgcatt gctccagaat atgaagcatt tgccaaggct gctgaagcag
     2101 ctggatttcc attagtgatg gtctcagctt accggtctgt atcatcccaa caacaagttt
     2161 tcgaacaaaa tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa
     2221 agattactaa agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag
     2281 tcgatgtcgt cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt
     2341 acggagatca gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg
     2401 ttcgttatcc aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac
     2461 gttatgtcgg taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat
     2521 atctgaatga attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc
     2581 aaaagcgcaa gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag
     2641 cctttgtttt ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg
     2701 aagcgcaatc ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag
     2761 atggttatgg agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg
     2821 gaaaaagtac cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc
     2881 agaagaaagt aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag
     2941 gagacttcaa agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg
     3001 tccaaggagt agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc
     3061 aggaagccgc acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa
     3121 aaatcatcca ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga
     3181 gaagtggctg atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat
     3241 cgtaagagta ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat
     3301 ccgttcgatc atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt
     3361 aattgctgtg tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc
     3421 tgacattcca gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta
     3481 catgagcatg ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc
     3541 tttagagaca gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac
     3601 gtcgaaatat acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag
     3661 tgcaggtaaa gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc
     3721 tatcattgcg acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc
     3781 aaatgccatc acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa
     3841 atatcgggaa actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag
     3901 agcagatagt ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa
     3961 aaaagagaaa tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt
     4021 gttattatta gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat
     4081 gaaaaacgaa taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg
     4141 gcttgaaaag tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga
     4201 cgtgtatatt gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa
     4261 gagcgcacac gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga
     4321 tatccttttt tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga
     4381 tgaaagaaga atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc
     4441 agaacgataa ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg
     4501 ttctgtataa aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga
     4561 agaaaagaat gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg
     4621 ggcaagaaga tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac
     4681 acgatgcaac agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg
     4741 attatgaatt ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag
     4801 atgatatctg ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg
     4861 aaccattaga tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct
     4921 atttagcaac gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt
     4981 ttgcgattgc ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa
     5041 attggacatg ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct
     5101 atgctatttc aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg
     5161 agaaaggatt gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc
     5221 cagactatct aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc
     5281 cagaaccagg tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata
     5341 gagaagcggc aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc
     5401 ctgaagatat ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg
     5461 gtatcccttt acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag
     5521 aagctgctaa gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag
     5581 gagaacgtgg tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg
     5641 caactttgca agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag
     5701 tgaatgtctt tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg
     5761 aaaaagtcat ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg
     5821 cgtcaaacat tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt
     5881 tcttggatca agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc
     5941 tattgataac agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt
     6001 tgggaaatcc agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc
     6061 gttttttgaa ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac
     6121 tgatctttgg tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct
     6181 ttcgttcttt gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt
     6241 ggcgggagtt gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac
     6301 ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg
     6361 cttggcaatt cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct
     6421 cgtattatga agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca
     6481 cgttacctct tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt
     6541 ttatgacttt tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact
     6601 tttatgctct ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg
     6661 ctatttcgtg gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat
     6721 caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa
     6781 tggtttcaat acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg
     6841 atgatcgcag gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt
     6901 ccagagactt ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca
     6961 ttttttactt tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt
     7021 ctgagctcga cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc
     7081 tggttcgtcg tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag
     7141 tttattattt acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat
     7201 ttttttggac aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa
     7261 gagttagatg aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt
     7321 atttttcctt tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa
     7381 tgggacgact tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg
     7441 attgccatca aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg
     7501 atgtctacgt tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt
     7561 gtccagggga tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga
     7621 tgtttcttta caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta
     7681 atgctctttt cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc
     7741 gtgttgtcta tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt
     7801 acttggcaga aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca
     7861 ttacgttgtt tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa
     7921 agattggagc ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata
     7981 gtttttttag tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt
     8041 tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat
     8101 gccttttgta tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt
     8161 atccagggct gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat
     8221 gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat
     8281 tgcctctacg tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag
     8341 gatggattat gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg
     8401 tatgccatca caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac
     8461 gtcagtagat cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt
     8521 tatttgcgaa aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca
     8581 aaaacagacg ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac
     8641 gaagataaag gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga
     8701 atggctgtta aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca
     8761 caatagcggc gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg
     8821 gttgaacagc cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca
     8881 taatgcggag aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc
     8941 taaagctgat ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt
     9001 agaagcagaa aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc
     9061 tattgaagat acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc
     9121 gctaaacgcg tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg
     9181 gatcgaaatg aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga
     9241 aggagcagcc gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt
     9301 aatcgtcgaa caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct
     9361 atttggtgaa gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat
     9421 tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt
     9481 gattgacgag cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta
     9541 gtttataaaa tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa
     9601 aaggggctat gacatgcttt ttgtcatgcc atctttttat gtgggctgct tttttagata
     9661 ggagaaaata aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat
     9721 tagattttac aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg
     9781 ttttcaatat ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa
     9841 acagttgcaa gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt
     9901 cataatatgg atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt
     9961 ggctatttca cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca
    10021 tagtaggttt ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata
    10081 aagtgaaaga attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat
    10141 taaaagtcga cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa
    10201 aacggtttgc agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt
    10261 ctgggatcgc accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta
    10321 gaaaagcaaa aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta
    10381 cgaaacaagt gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag
    10441 tattgattat cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat
    10501 gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag
    10561 atggacgtca gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt
    10621 ccttgacgaa tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac
    10681 acaaaatgga aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc
    10741 agtagcggtg cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta
    10801 tttgatttca attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa
    10861 ccgatttttc ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc
    10921 actgatcttg atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc
    10981 tgggatcttt gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc
    11041 tatagtaaca ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga
    11101 gaaaatgggt ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact
    11161 tgcatttgtg acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg
    11221 ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat
    11281 gattgatctg agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt
    11341 tggtaaacca actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag
    11401 catggtagaa tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg
    11461 agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat
    11521 tttcaataca ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg
    11581 aatcaaaaca agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt
    11641 ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt
    11701 agtcatgttc gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag
    11761 caatgataaa aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt
    11821 tacgccgaca ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat
    11881 cgtaatgagt agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga
    11941 tgaagaaata ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc
    12001 agcgttttga cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt
    12061 ttgtttgagc taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag
    12121 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt
    12181 gaatattgtt ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga
    12241 atgtgaccta acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa
    12301 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
    12361 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
    12421 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
    12481 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
    12541 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
    12601 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
    12661 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
    12721 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
    12781 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
    12841 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
    12901 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
    12961 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
    13021 tggcacattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
    13081 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
    13141 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
    13201 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
    13261 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
    13321 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
    13381 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
    13441 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
    13501 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
    13561 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
    13621 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
    13681 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
    13741 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
    13801 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
    13861 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
    13921 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
    13981 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
    14041 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
    14101 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
    14161 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
    14221 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
    14281 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
    14341 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
    14401 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
    14461 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
    14521 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
    14581 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatctctact
    14641 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
    14701 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
    14761 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
    14821 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
    14881 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
    14941 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
    15001 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
    15061 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
    15121 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
    15181 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
    15241 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
    15301 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa tcgagtagag
    15361 gggaaagcca actgaccttc cgcccctctc acaccaccgt acgtacggac ccgtatacgg
    15421 cggttcaata agttgagtgt acgaactggt acttcaagga gatgtcctca tatccttggc
    15481 gggccagttc tttgtctgtt aatgatcgat gaagaatatg acttttggag actgaccaaa
    15541 taccttttct tgtatttgac cattcatacg ctttcgcttt atcgatccct aaacgaatca
    15601 gattttttct tttggttctt ggattcttcc attgtttcca aatatattgt ctgattcttc
    15661 gcttcaacca accgtttaac tccctcatga attctttcat ctcaccaatt ccatagtagt
    15721 tgatccaacc agtcattagt tgcttgattt cttttaaaat aagttggata cttcttccac
    15781 ggtttctttt agtgagttgt ttgagcttgc ttttcacacg ctgttttgct tttgcgtgag
    15841 ggcgaatttt aatacctttc tttgttggca gaatgcagaa tccaagaaac tttcgtttca
    15901 atggacttcc taccgcactc ttttctcgat ttaccgtcaa ctttagttgt tcttcgagaa
    15961 attgaatgga tttcttcatc acacgctgtc ctgctcgttt gcttctaaca tagatattgc
    16021 agtcatcggc gtatcgaaca aatttatgtc ctagattttc tagcacttga tcaaattcgt
    16081 tcaggtagat gttacttaac aggggagaga gattgcctcc ttggggcgtt ccttcttcag
    16141 ttgttgtgat gagtccattt tccattacac cactcattaa gtattgacgg atcagtttta
    16201 ggacttgttt gtcctttact ttccgctcaa tgaaatacat cagcttatcg tgatttacgg
    16261 tgtcaaagta ggctttcata tcaatgtcca ccacatatcg gtaaccttgt tcataataag
    16321 attttgcttt cttcactgcg tcatgtgcgc tgcgatttgg gcgaaaccca taactattat
    16381 ctgagaattc tggttcaaat agcggttgta acacttgatt gattgcctgt tgaagcatac
    16441 gatcaataac cgtgggaatt cctagttttc ttacaccgcc gtttggtttc ggtatttcta
    16501 ctcgcaaaac aggctgaggt ttatagttcc cttggcgtaa attggatagt aattcctcgc
    16561 ggtgctcttt caagtaagga agcagttgat cgacggtcat cccatctgca ccagccgccc
    16621 ctttatttct gactacttga agatacgctt gattgagatt attccgatct agaattctcc
    16681 caaacagatt ttctacacca tcttgtttcc tctgttcacc gcaagaagtg ctgcgcactt
    16741 catcataccc ttcgtgttcc acactagctc tttgatgata gccaagtttt cttgttttct
    16801 gcggtcgtcg catcttctca cacctcctaa attattcaag attcttattg ttcagtcctt
    16861 cgtaacggtc agtgtcgtta ctactatgac ctcggctgac ttctcatgat tcaccatcat
    16921 gtcgccatga tcgttgtgct ggtttgtttt cattcaacgc ccacaacacc ctcatgagat
    16981 ctcccccggt aagagtgaaa actttcctcc catgtaactg ctagatttac tgtataggtt
    17041 tcgggcagta ttggactttg tcttgtgatg aagacttatc cgacctaatt cagccttgta
    17101 tctagtttct gttcgtcagt tcaggagttt gcgtccgact tccttcagcc acaacctcac
    17161 gattgccgcc ttgtctttcg ctaacagttc ctactgccaa gcctgtagtg gactttcacc
    17221 accaagtttt cacccatgca gggcgcacac caaaaaagaa gcagatccgt tagcggttct
    17281 gcttctttta gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct
    17341 acctctttac cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg
    17401 ctattataac taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat
    17461 tccatctctt gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt
    17521 tcatgaatct gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt
    17581 ttacggcatt cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta
    17641 gtatctttat tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa
    17701 cgttcttcag ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc
    17761 ggcaagtgcc tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt
    17821 aagtataaat gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac
    17881 attaaaagaa tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg
    17941 ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa
    18001 aaggagagac gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat
    18061 atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga
    18121 atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg
    18181 caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta
    18241 aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata
    18301 ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac
    18361 caatcattga attctactat aaggatgacc agttggatga tccatttatc aatgaagagc
    18421 atatctattt cttacaactt gcaaagccgg aagaaatcag tgagctgaaa gcccaagcgt
    18481 tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt
    18541 tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg
    18601 atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca
    18661 gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa
    18721 ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc
    18781 agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac
    18841 aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc
    18901 aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac
    18961 ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt
    19021 ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg
    19081 atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc
    19141 cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa
    19201 tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg
    19261 gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt
    19321 tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag
    19381 agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct
    19441 gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg
    19501 acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt
    19561 taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga
    19621 aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat
    19681 tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga
    19741 ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg
    19801 aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg
    19861 ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag
    19921 gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc
    19981 cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata
    20041 ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag
    20101 ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca
    20161 attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa
    20221 acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc
    20281 aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta
    20341 tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg
    20401 ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa
    20461 tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt
    20521 catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag
    20581 tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac
    20641 ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt
    20701 atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc
    20761 atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt
    20821 atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc
    20881 agccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg
    20941 cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc
    21001 gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga
    21061 ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag
    21121 tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact
    21181 gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg
    21241 acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt
    21301 tatataatga aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat
    21361 tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct
    21421 atatattagg taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg
    21481 gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt
    21541 tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg
    21601 gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt
    21661 ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg
    21721 caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta
    21781 aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa
    21841 ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt
    21901 aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata
    21961 cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga
    22021 tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca
    22081 aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc
    22141 aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat
    22201 cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga
    22261 aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg
    22321 aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg
    22381 ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt
    22441 tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg
    22501 tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat
    22561 catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg
    22621 ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca
    22681 tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat
    22741 ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg
    22801 aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc
    22861 aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt
    22921 ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg
    22981 gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc
    23041 tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc
    23101 ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt
    23161 aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt
    23221 gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata
    23281 tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata
    23341 tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag
    23401 tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta
    23461 taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg
    23521 tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg
    23581 atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag
    23641 agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa
    23701 atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag
    23761 aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg
    23821 tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt
    23881 tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt
    23941 tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg
    24001 gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag
    24061 aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc
    24121 gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa
    24181 tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca
    24241 atatgggcat cggtatggtc ctggcagtcc aagaatcgaa agtaaaagaa atagtacacg
    24301 aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac
    24361 atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg
    24421 aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga
    24481 ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt
    24541 accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat
    24601 tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat
    24661 cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct
    24721 tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga
    24781 aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca
    24841 ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag
    24901 agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct
    24961 gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa
    25021 ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat
    25081 aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat
    25141 ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc
    25201 cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc
    25261 aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt
    25321 gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt
    25381 gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat
    25441 gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg
    25501 gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa
    25561 aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa
    25621 gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa
    25681 ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc
    25741 atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt
    25801 gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca
    25861 atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg
    25921 cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta
    25981 ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa
    26041 aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca
    26101 atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca
    26161 aaagcattag cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg
    26221 gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta
    26281 aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct
    26341 tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt
    26401 cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta
    26461 gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac
    26521 attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt
    26581 ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc
    26641 aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag
    26701 gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat
    26761 tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt
    26821 tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga
    26881 agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta
    26941 gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa
    27001 gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa
    27061 atcgtcgtag aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat
    27121 accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa
    27181 gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg
    27241 attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga
    27301 cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg
    27361 atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag
    27421 agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg
    27481 cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat
    27541 gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga
    27601 gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca
    27661 tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg
    27721 acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc
    27781 gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg
    27841 attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg
    27901 aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat
    27961 cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta
    28021 tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga
    28081 aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac
    28141 aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc
    28201 tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg
    28261 tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc
    28321 aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa
    28381 aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg
    28441 aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg
    28501 tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt caccatacca
    28561 gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac
    28621 attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat
    28681 tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa
    28741 ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg
    28801 tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt
    28861 aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt
    28921 gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga
    28981 aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga
    29041 aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt
    29101 gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt
    29161 ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag
    29221 cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat
    29281 ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag
    29341 aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt
    29401 gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat
    29461 gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt
    29521 gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat
    29581 caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat
    29641 cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga
    29701 tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa
    29761 aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg
    29821 tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa
    29881 cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat
    29941 gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt
    30001 tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg
    30061 agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag
    30121 aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt
    30181 tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc
    30241 ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta
    30301 gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca
    30361 gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt
    30421 cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat
    30481 tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt
    30541 gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca
    30601 atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa
    30661 agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct
    30721 gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca
    30781 tcaattttgc atgtggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg
    30841 tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt
    30901 gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact
    30961 agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt
    31021 aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag
    31081 tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc
    31141 tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc
    31201 cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat
    31261 tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac
    31321 atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt
    31381 ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc
    31441 gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct
    31501 tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg
    31561 gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt
    31621 gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta
    31681 tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca
    31741 tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc
    31801 ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc
    31861 tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct
    31921 ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag
    31981 tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat
    32041 aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt
    32101 attggctaca taccagccga catcaggttt acactcgact acacaattga cgattcctga
    32161 gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc
    32221 aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg
    32281 gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc
    32341 ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt
    32401 atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc
    32461 ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat
    32521 ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc
    32581 tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg
    32641 tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca
    32701 cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg
    32761 tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc
    32821 ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt
    32881 tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca
    32941 ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt
    33001 aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc
    33061 aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac
    33121 acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc
    33181 tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt
    33241 gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga
    33301 tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga
    33361 tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat
    33421 cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca
    33481 aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta
    33541 gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca
    33601 gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct
    33661 tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt
    33721 ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta
    33781 caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa
    33841 atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc
    33901 gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta
    33961 ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg
    34021 attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca
    34081 caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca
    34141 atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa
    34201 actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag
    34261 ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag
    34321 gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa
    34381 tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga
    34441 ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga
    34501 tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg
    34561 gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc
    34621 atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg
    34681 aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc
    34741 tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc
    34801 aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg
    34861 atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag
    34921 agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg
    34981 atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg
    35041 aaatgatcag cgaagagcat attattcgcg gaatggtggg cagagattta gttgatcgtt
    35101 atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct
    35161 atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag
    35221 gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc
    35281 tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga
    35341 tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc
    35401 gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt
    35461 tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa
    35521 atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta
    35581 gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc
    35641 tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga
    35701 ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg
    35761 aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag
    35821 tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg
    35881 gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa
    35941 agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt
    36001 attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt
    36061 gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta
    36121 ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg
    36181 atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc
    36241 gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc
    36301 gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa
    36361 tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta
    36421 agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg
    36481 ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa
    36541 tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact
    36601 tatatctttg cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt
    36661 gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc
    36721 ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta
    36781 ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat
    36841 gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt
    36901 ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg
    36961 gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag
    37021 gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa
    37081 cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac
    37141 agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata
    37201 tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga
    37261 gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc
    37321 tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt
    37381 aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg
    37441 taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac
    37501 aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc
    37561 agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt
    37621 tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc
    37681 tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca
    37741 gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga
    37801 agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg
    37861 aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc
    37921 tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac
    37981 acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt
    38041 gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa
    38101 agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa
    38161 gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga
    38221 ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga
    38281 ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat
    38341 attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata
    38401 gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca
    38461 ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa
    38521 aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa
    38581 gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg
    38641 gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca
    38701 gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa
    38761 gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag
    38821 actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt
    38881 tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca
    38941 tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt
    39001 ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat
    39061 actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc
    39121 cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg
    39181 attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct
    39241 cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac
    39301 ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg
    39361 aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa
    39421 ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt
    39481 ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc
    39541 ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag
    39601 acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt
    39661 acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta
    39721 agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt
    39781 ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa
    39841 ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta
    39901 ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa
    39961 ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac
    40021 aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt
    40081 ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc
    40141 acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat
    40201 agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aatataaaac
    40261 gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc
    40321 tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt
    40381 aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag
    40441 attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga
    40501 cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca
    40561 agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga
    40621 attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg
    40681 ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag
    40741 tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact
    40801 agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg
    40861 taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata
    40921 tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt
    40981 cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc
    41041 tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac
    41101 acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat
    41161 tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc
    41221 acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta
    41281 tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga
    41341 cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact
    41401 ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc
    41461 tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt
    41521 cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa
    41581 ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta
    41641 gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc
    41701 cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta
    41761 gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt
    41821 cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg
    41881 gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca
    41941 tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct
    42001 cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg
    42061 atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc
    42121 tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt
    42181 actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc
    42241 aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca
    42301 gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt
    42361 gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca
    42421 gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc
    42481 ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa
    42541 gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa
    42601 tttaaatt
//
LOCUS       DASJPH010000011        40712 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJPH010000011 DASJPH010000000
VERSION     DASJPH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.011"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            77..514
                     /gene="zapA"
                     /locus_tag="U3995_000560"
     CDS             77..514
                     /gene="zapA"
                     /locus_tag="U3995_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1795909.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            572..1120
                     /locus_tag="U3995_000561"
     CDS             572..1120
                     /locus_tag="U3995_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN1795910.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            1235..3595
                     /locus_tag="U3995_000562"
     CDS             1235..3595
                     /locus_tag="U3995_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1795911.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            3682..3996
                     /gene="trxA"
                     /locus_tag="U3995_000563"
     CDS             3682..3996
                     /gene="trxA"
                     /locus_tag="U3995_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1795912.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            4178..5956
                     /gene="uvrC"
                     /locus_tag="U3995_000564"
     CDS             4178..5956
                     /gene="uvrC"
                     /locus_tag="U3995_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1795913.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            6101..7000
                     /locus_tag="U3995_000565"
     CDS             6101..7000
                     /locus_tag="U3995_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1795914.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            6993..8255
                     /locus_tag="U3995_000566"
     CDS             6993..8255
                     /locus_tag="U3995_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1795915.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            8416..10314
                     /locus_tag="U3995_000567"
     CDS             8416..10314
                     /locus_tag="U3995_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN1795916.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(10644..11363)
                     /locus_tag="U3995_000568"
     CDS             complement(10644..11363)
                     /locus_tag="U3995_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1795917.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            11700..12032
                     /locus_tag="U3995_000569"
     CDS             11700..12032
                     /locus_tag="U3995_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795918.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            12019..14022
                     /locus_tag="U3995_000570"
     CDS             12019..14022
                     /locus_tag="U3995_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1795919.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            14041..14511
                     /locus_tag="U3995_000571"
     CDS             14041..14511
                     /locus_tag="U3995_000571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1795920.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            14532..15116
                     /locus_tag="U3995_000572"
     CDS             14532..15116
                     /locus_tag="U3995_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1795921.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            15131..16117
                     /locus_tag="U3995_000573"
     CDS             15131..16117
                     /locus_tag="U3995_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1795922.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            16107..16418
                     /locus_tag="U3995_000574"
     CDS             16107..16418
                     /locus_tag="U3995_000574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1795923.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            16440..18221
                     /locus_tag="U3995_000575"
     CDS             16440..18221
                     /locus_tag="U3995_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1795924.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            18214..19590
                     /locus_tag="U3995_000576"
     CDS             18214..19590
                     /locus_tag="U3995_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1795925.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            19597..20232
                     /locus_tag="U3995_000577"
     CDS             19597..20232
                     /locus_tag="U3995_000577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1795926.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            20262..21617
                     /locus_tag="U3995_000578"
     CDS             20262..21617
                     /locus_tag="U3995_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1795927.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(21809..23203)
                     /locus_tag="U3995_000579"
     CDS             complement(21809..23203)
                     /locus_tag="U3995_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1795928.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            23295..24266
                     /locus_tag="U3995_000580"
     CDS             23295..24266
                     /locus_tag="U3995_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1795929.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            24263..25045
                     /locus_tag="U3995_000581"
     CDS             24263..25045
                     /locus_tag="U3995_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1795930.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(25214..25585)
                     /locus_tag="U3995_000582"
     CDS             complement(25214..25585)
                     /locus_tag="U3995_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1795931.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            25719..26162
                     /locus_tag="U3995_000583"
     CDS             25719..26162
                     /locus_tag="U3995_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1795932.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(26205..27014)
                     /gene="yidA"
                     /locus_tag="U3995_000584"
     CDS             complement(26205..27014)
                     /gene="yidA"
                     /locus_tag="U3995_000584"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1795933.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(27033..28400)
                     /locus_tag="U3995_000585"
     CDS             complement(27033..28400)
                     /locus_tag="U3995_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1795934.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(28384..29214)
                     /locus_tag="U3995_000586"
     CDS             complement(28384..29214)
                     /locus_tag="U3995_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1795935.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            29377..29814
                     /locus_tag="U3995_000587"
     CDS             29377..29814
                     /locus_tag="U3995_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1795936.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            29890..30510
                     /locus_tag="U3995_000588"
     CDS             29890..30510
                     /locus_tag="U3995_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1795937.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            30699..31205
                     /locus_tag="U3995_000589"
     CDS             30699..31205
                     /locus_tag="U3995_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1795938.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            31222..32121
                     /locus_tag="U3995_000590"
     CDS             31222..32121
                     /locus_tag="U3995_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1795939.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            32208..33092
                     /locus_tag="U3995_000591"
     CDS             32208..33092
                     /locus_tag="U3995_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1795940.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(33284..33355)
                     /locus_tag="U3995_000592"
     tRNA            complement(33284..33355)
                     /locus_tag="U3995_000592"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33320..33322),aa:Arg,seq:ccg)
     gene            33415..34749
                     /gene="rpoN"
                     /locus_tag="U3995_000593"
     CDS             33415..34749
                     /gene="rpoN"
                     /locus_tag="U3995_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1795941.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            34898..35989
                     /locus_tag="U3995_000594"
     CDS             34898..35989
                     /locus_tag="U3995_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1795942.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            36030..37031
                     /gene="gap"
                     /locus_tag="U3995_000595"
     CDS             36030..37031
                     /gene="gap"
                     /locus_tag="U3995_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1795943.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            37141..38331
                     /locus_tag="U3995_000596"
     CDS             37141..38331
                     /locus_tag="U3995_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1795944.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            38435..39190
                     /locus_tag="U3995_000597"
     CDS             38435..39190
                     /locus_tag="U3995_000597"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1795945.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            39306..40604
                     /gene="eno"
                     /locus_tag="U3995_000598"
     CDS             39306..40604
                     /gene="eno"
                     /locus_tag="U3995_000598"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1795946.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        13534 a         7085 c         8716 g        11377 t
ORIGIN      
        1 ttgtggagaa cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata
       61 ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac aaagcggtga ttgccaacca
      121 aacatatact attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat
      181 caatgaacag ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc
      241 aatcttgatg gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatattgga
      301 gctggaagaa gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa
      361 tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg
      421 tcaagcagat cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg
      481 aaaaggacaa attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat
      541 ttaaaaaagt tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt
      601 gctaattgcg tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat
      661 tggatatgta ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt
      721 ggaattatac attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca
      781 ggtatttgcc tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt
      841 gctaattggc tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt
      901 gccgatcttg agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta
      961 cgttactata tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa
     1021 tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa
     1081 caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat
     1141 atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca
     1201 gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt
     1261 ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga
     1321 agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga
     1381 aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaaattga
     1441 aaatatccgt ccccatatga aacggattga gatcggagca gacttgaatg gagtggagct
     1501 tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct
     1561 ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat
     1621 ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga
     1681 cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg
     1741 tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat
     1801 caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg
     1861 cggtgtagtc cacgatcaaa gtgcttcagg tcagacttta tttattgaac caaaacaagt
     1921 ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg
     1981 tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatac
     2041 ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa
     2101 agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc
     2161 actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc
     2221 catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct
     2281 gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg
     2341 tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc
     2401 gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag
     2461 tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc
     2521 aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta
     2581 tcctgaattg aaagtttatg gatataatcg ggcaaatacg attaatgcca gcatggagtt
     2641 tgatgtggat acattaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa
     2701 tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca
     2761 gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg
     2821 gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact
     2881 tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga
     2941 aaaagcaaag aaaaaagcaa atgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat
     3001 cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca
     3061 gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa
     3121 taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt
     3181 tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga
     3241 aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa
     3301 agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa
     3361 tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt
     3421 ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg
     3481 cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga
     3541 gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt
     3601 actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta gtcatagatt
     3661 ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa
     3721 acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg
     3781 caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct
     3841 aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca
     3901 acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa
     3961 gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga
     4021 gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa
     4081 attaatattt tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc
     4141 tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa
     4201 attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat
     4261 catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggtag
     4321 ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac
     4381 agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa
     4441 atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa
     4501 atatccccgt ttggtgatta ctcgtaaggt actaaaagac aaagcactgt attttggacc
     4561 atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct
     4621 gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg
     4681 cctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt
     4741 gaaaaacttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga
     4801 aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc
     4861 cattgagaca atcatgactt gccaaaaaat gacaaatact gatttactgg atcgagatgt
     4921 atttggttat gcgattgata aaggatggat gtgtgtccaa gtgtttttcg taagacaagg
     4981 aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt
     5041 cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt
     5101 gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca
     5161 accaaaacga ggagaaaaga aaaaattagt tgaattggcg agtaagaatg cctcagttgc
     5221 tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga
     5281 acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc
     5341 taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc
     5401 aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc
     5461 aatgagagaa gtcatctatc gcagatattc gcgagtgatt cgcgaagggt tgcctttacc
     5521 tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga
     5581 aaatcaatta ggaatcgata ttcctattgc tggcatggta aaaaacgata aacataaaac
     5641 aaatgagttg ctattcggta cagatttggc tgtagtaccg cttgagcgaa attcaccaga
     5701 gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg
     5761 gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc
     5821 aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac
     5881 gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa
     5941 attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta
     6001 tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg
     6061 tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct
     6121 tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg
     6181 aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat
     6241 cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga
     6301 gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac
     6361 gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga
     6421 accaaaaatc gacttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt
     6481 aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga
     6541 acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt
     6601 gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca
     6661 caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat
     6721 ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt
     6781 ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct
     6841 tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt
     6901 tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga
     6961 agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag
     7021 atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta
     7081 tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata
     7141 aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag
     7201 atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag
     7261 aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt
     7321 atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct
     7381 tgcaatcgat gcttcgagcg agtcaattag gcatttcacc agaagaagta gctagcttga
     7441 gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag
     7501 tcggagaaga taactcgaca gtccaatacg tcgtaagtta tgtagcaacg attgtcttgt
     7561 tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca
     7621 cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaat
     7681 taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta
     7741 taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat
     7801 tggataactt gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt
     7861 tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag
     7921 caaaagcaat tttgccagtg acttatctat ctctaggcgg atacatgctt ggattgatac
     7981 taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat
     8041 cttcatatat tatgccgatt cgcttagcaa atgagacagt cggtatgggt ggagcattga
     8101 tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt
     8161 acaaatcgaa tgtactcgtt tatagcgaag gcggattatg gagttcattg aaacaatcga
     8221 tttcaattat gaggaatgaa cgaaaaaaag cataaaacaa tcctaaaata cagaaggaag
     8281 ctggacatct attgttccca gcttcttttt gtatagacct attttattta ttttactata
     8341 ttggatatat attcattgaa aatagtgtat aacaacttta taatattgag tataccaatt
     8401 tttggaggcg ttttaatgga ccgttattta gatttacttt cagaaaaatt cccctcaacg
     8461 gaggatgtca tcacggaaat catcaatctc gaagcaattt tgcagcttcc aaaaggaaca
     8521 gagttatttt tgagtgatat acatggcgaa tttcctgctt ttgatcatat tctgcgtatc
     8581 ggttcaggaa atcttaaaga aaagatcaaa gacctttttt ctgaacaaat gacggaaacg
     8641 gatatcagcc aattcactat ttttacggct tatccagaat atgcattaac gactgattgg
     8701 tacaaagaac aagataaatc gcagctgatc catcatttga tcgatctttt acggtttacg
     8761 acagtcaaat atacccggtc aaaagttcga aaaggattgc cgaaagaata tagctatatc
     8821 attgaggaac tcttgtatat cgatgaccgt atcaatggaa aaaaagacta tgtaaaaaaa
     8881 atcattgaac aacttgtttt gatgaaggaa gaagaaaaat ttttaacgaa acttgcacag
     8941 acgatccaaa aatctgtcat cgatcatttg catatcgttg gagatatttt tgaccgagga
     9001 actcaagcag ccaaagtgat ggatcgaatc atggattttt cttctgtaga tatccaatgg
     9061 ggaaatcatg atattttgtg gattggtgcg tattgcggct ctgaggcatg tttactgaca
     9121 cttttacgga ttgcagcacg ctataattat ttatttgaat tagagaaaga atatgggttg
     9181 aatttacgtc cattgttttc ttttgctcat caaacttatc agaagaatcc agtatttgcg
     9241 cctaaaaaca gtaaaaatct atccgaaaga gagcaagaag aattagaaaa aatccatcag
     9301 gcactttcta tccttcaatt caaatcagaa catcagttat tggatcgtcg acctgaattt
     9361 aaaatggacg atcgtaaatt gctggaaaaa atcgactatg aagcatcaac gattgattta
     9421 gaaggtcagc tctatgagtt gaaaggaact tgttttcaaa cgatccagcc agatgatcca
     9481 aaaaaattct tggaagagga acaaaaagtg attgatagtt tgatgtattc attccagcac
     9541 tctttgagaa tgcaaaaaca tatgacgttt ttgatcgaaa aagggggaat gtatctcacg
     9601 aacaatcagc atgtgctgtt tcatggctgt atacctgtag acgagaaggg acagctctta
     9661 gcatttgaat tagaccaatc ttatcgcgga aaagaactgc tgggcttttt tgaaaaacag
     9721 ataactgaaa gcgcaaaaga tctgactgca aaagaagatt tagcaaccga tttgatttgg
     9781 tatgcgtgga gtgggccatt ttcgccttta ttcggcaaga gtaaaatggc gacttttgaa
     9841 cgttattttc tcacagagcc tcatacgcac gtcgagaaaa gcaatcctta ttatcacttg
     9901 cgagatgaag agtggttcag tgaaaaaatt ttagcagaat tcgacacaaa agaagaaggg
     9961 tcagcaatca ttaatgggca cacgccagta aaagtaaaaa aaggggagtc accgatcaaa
    10021 gcaaatggaa aagtatttgt cattgacgga ggtttatcca aagcttatca aaaaacgaca
    10081 ggaattgccg gttattcact attatgcaat tcttatggtt tccaaatcgt gacacattat
    10141 ccgtttttgc ctattgaaca acaatttgca aaaaaatccg accaaacaaa tgtgaaaaaa
    10201 gtaatcgaac agcaactacc acgcaaactc aatcgtgata caacaaaagg tatggaactg
    10261 cagcagcaga ttacccagct caaaaggctg ttggactatc gaaaggacga ttgagaagaa
    10321 ttccagtgaa cattgttcca tagactggag actgagacaa aagtcgtgtc gcagtctctt
    10381 tttccggata aacggtgttc ttctgccaac tcctcggtca caagtcacac tattcaacaa
    10441 acatgaaaag ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac
    10501 gacctcttgg tataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc
    10561 aaaaatgtga aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt
    10621 tcacaacccc ctcagaaagt ctattaaaat tcattcacaa agaaatcgaa taagcgctgt
    10681 tcagatggat gtacacggtg ggtaagttcc ggatgccatt gaacaccgag gattttatgc
    10741 atatcatctt tgctttgaac cgcttcaacg agtccatcgt tagaaaaagc gatggctttt
    10801 aataaaggtg aaagatcttt gactgcctga tgatggtaag aattcacaaa atatggatcg
    10861 gggagaaggt tgcttaacaa actgtctgaa acaatcttga cttcgtgagt cgtaaattgc
    10921 ggggcagtgg gctgttgttc atgttttact gaccactttg gatataatga aagatcttga
    10981 taaagtgtac cttccaaaac gacattcaat agttggagtc cccggcagac tgcaaaaatt
    11041 ggttttcttt gtttcaatgc ttccaagatc agtgcctgtt caaattggtc tcgttgaata
    11101 tttgtttccg ttagctttgg atgaggatct tccatgtaaa gttgtggact gacatcttga
    11161 ccacctgcaa gaataagttt gtcgatttgt ccaatcagtt gaggagctga atctggagac
    11221 atgactggca gaataagagg caatccttga gcatctaaaa ctgcatccac gaatccttgc
    11281 ggtgtatagc tcacttgatt tccttggaaa gtatctgttg ctcgaatcag ttgatttccg
    11341 gcaatgccga tgattggttt catttgtatt ccccctaaag tagacttatt gtaacaagga
    11401 tttaaaaaaa attaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta
    11461 ctatctgtaa agagaaattt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat
    11521 ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt
    11581 tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta
    11641 gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag
    11701 tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgaaaagc
    11761 gtgctcaaac actgcggcag cagctgaaga tctatgcaac agaaaaagag caggaattgg
    11821 aaacgatcca aacaaactat cgacaacaac taaaacagtt ggtggaagaa aaagaacaat
    11881 ttgaaataca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc
    11941 aagcaacttt gaaagatcaa tatcaaaagc atcagagtca agcgatcggt gcgatcattg
    12001 aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat
    12061 aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaacgt tgaaatccgg
    12121 gatctgtatc aaaatagaac aaacaaccag tgggtggaga agtattttcc agataccacg
    12181 attattgata aagaagcaaa attaagcgct ttaggtaatc ggttgaaaga catccaagaa
    12241 gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt
    12301 caagaacttt ccttacaaga tttggaagcc aattattccg aagaaaattt cataaataaa
    12361 ttgaaagaag tccttgattt aaaagaaaag tgggaacaac taactgaaga acgtgatcgt
    12421 ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg
    12481 tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt
    12541 ttcaaggaag aactgagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca
    12601 aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgttat agaagaaatt
    12661 gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa
    12721 ctgcaagaga ccaagcagca attatccatt gttcacgagc agcaaaagaa gataacgaca
    12781 gcgttaggtc agtgcagtgg ctatatccaa gaatttgaat gggtagaaga agtcacactt
    12841 gccgttgcag aacgagaaaa aataaaagaa cggtttatcc atgcgactta tctgatcgtt
    12901 ttgcaaggtt gggtagatgc agaagaaaaa cagaatttgc tgcatgtgtt aaacgaaacc
    12961 gtatcagaag aagatatata cgtcagtttc gaaacaccaa actcagaaga gattcagcag
    13021 gaagtcccta ctaaattgaa aaaccatccg cttgtcgaac cttttgaatt gctgactgaa
    13081 atgtacagtc tgccaaaata tgaagaagta gatccaacac cttggatgac accattttat
    13141 cttgtcttct tcgggatgat ggtcgcagat gttggttatg gtttgctgat gctcattggg
    13201 acaatactag ctcaaaaact acttgttcta ccacgcggga tgaaacgttt cgtaaaattc
    13261 tttgagattc tctcgatacc atcgatcatc tggggattga tctacagttc atttttcgga
    13321 cagtcattac caaaagaact gtttggcatc cgcttgcctt tcccaatcct atctacgacg
    13381 gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca gattttggtt
    13441 ggattatttg tggcagcaaa ggaaaatatc aggagaaaag attacttgag tgctgtcagt
    13501 gatggatttg cctggcaagg gatcctaatt gggatcgtta ttgcgttggt tggtgccatg
    13561 ttgataaaaa atacagcatt tgtttacctt ggcgctgggc tatcgatcat tgctgcctta
    13621 tgtatcttga tcgttccgat tatccaaacg ccgtctaaag tcaaaggtgc tgccaaaggt
    13681 gcgtataatc tttacggact gaccggctat atcggtgatt tagtcagtta tacacgtttg
    13741 atggcattgg gtatttcagg cggaagtatt ggagcggcat tcaatatgct agttgctttc
    13801 atgccgccag ctgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg
    13861 aatatgtttt tgacactgct aagtgcctat gtccatggcg cacgattgca gtatgtcgaa
    13921 ttttttggga agttttatac aggaggcgga cgtgcctttg atccgctgaa aacggcggaa
    13981 aaatacgtca acatcaatca caagaaaaaa aacaataaat aattggagga tttttatata
    14041 atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcggtatt agcgatggca
    14101 acagcgacga ttttttcagg gatcggttca gctaaaggtg ttggaatgac aggtgaggca
    14161 gcagcagcat tgacaacaag tcaacccgaa aaattcgggc aggctctgat tctgcaattg
    14221 cttccaggca cgcaaggttt gtatggattc gttattgcct ttttgatttt tatcaactta
    14281 ggaaatgaca tgtcagttgt acaaggactg aactttctag gtgcttctct gccgattgct
    14341 tttacaggct tgttttcagg tattgcccaa ggaaaagtcg cagcggcagg tattcaaatt
    14401 ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca
    14461 tacgcgattt taggtttcgt tatttccttc ctattagtgt tgaatgccta agcataaagg
    14521 aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaacgaa gcagcagagc
    14581 aggaaagagc ggcgttagaa caagaagagc gaatgaagat cgatcaaaac tttgaacaaa
    14641 aacgtacgca agttgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattactcg
    14701 aaaaaaaata ccgtcagcta aggaatcgcc agcaagtaga agtacgacaa gaaaatttga
    14761 atgcgaagca ggaattttta agacgattgt ttgcagatgc ggtaactgaa atggaaaatt
    14821 gggatgaatc tgaacaaatc cagtttatca aaaatgcatt gtattcatta cctttgacag
    14881 gaaaagtagc attcattgct ggagaaaaat cagcagccta tctttctcag acgttgttgg
    14941 atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc
    15001 aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg
    15061 tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag
    15121 ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact
    15181 tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga
    15241 aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga
    15301 acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga
    15361 gcctgaaatc gtttggatct atacgatgcg ttatactttc cataatctga aggttttgac
    15421 aaaagctgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc
    15481 attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaacttc ctgaaagcat
    15541 tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat
    15601 tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg
    15661 atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat
    15721 ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc
    15781 gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac
    15841 gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa
    15901 tattgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga
    15961 agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt
    16021 agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg aatcagaaac
    16081 aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag
    16141 attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa
    16201 ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag
    16261 agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg
    16321 ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa
    16381 aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt
    16441 tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg
    16501 atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg
    16561 agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag
    16621 gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt
    16681 cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta
    16741 atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg
    16801 aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag
    16861 aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa
    16921 caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg
    16981 gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag
    17041 aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc
    17101 cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg
    17161 ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg
    17221 agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata
    17281 caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag
    17341 cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg
    17401 gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga
    17461 tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac
    17521 ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg
    17581 aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag
    17641 tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc
    17701 aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag
    17761 tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca
    17821 tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt
    17881 ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt
    17941 tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa
    18001 tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt
    18061 atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat
    18121 atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga
    18181 tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt
    18241 gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta
    18301 atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa
    18361 gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct
    18421 gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta
    18481 ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg
    18541 gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa
    18601 acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta
    18661 ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg
    18721 gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa
    18781 gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg
    18841 tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg
    18901 actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac
    18961 atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga
    19021 agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga
    19081 agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac
    19141 gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg
    19201 actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg
    19261 cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg
    19321 aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta
    19381 ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa
    19441 aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta
    19501 gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg
    19561 ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg
    19621 acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa
    19681 ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac
    19741 aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg
    19801 gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac
    19861 gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt
    19921 gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc
    19981 gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca
    20041 gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc
    20101 aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg
    20161 aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt
    20221 accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac
    20281 aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt
    20341 tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt
    20401 ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga
    20461 atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg
    20521 gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca
    20581 gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga
    20641 tgaagctcat gctttacatc ctaaaattgg cctttgtgat ccaattgatt ggtacggttg
    20701 ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt
    20761 tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca
    20821 atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt
    20881 taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc
    20941 attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct
    21001 ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa
    21061 acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc
    21121 atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg
    21181 ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt
    21241 cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag
    21301 tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga
    21361 ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg
    21421 tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa
    21481 tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg
    21541 tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa
    21601 gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa
    21661 aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt
    21721 gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata
    21781 agatttgatt cctttattta tcaataggtt ataattcata tttttttatt ttttctttta
    21841 cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga
    21901 atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag
    21961 caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat
    22021 agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg
    22081 ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga
    22141 tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt
    22201 gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac
    22261 aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag
    22321 gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa
    22381 cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt
    22441 gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc
    22501 tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga
    22561 tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg
    22621 ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac
    22681 cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg
    22741 ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa
    22801 gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa
    22861 aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga
    22921 tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac
    22981 ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat
    23041 cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat
    23101 cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg
    23161 gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa
    23221 attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga
    23281 ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg
    23341 aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc
    23401 aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga
    23461 tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg
    23521 ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa
    23581 acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg
    23641 tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg
    23701 gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta
    23761 cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa
    23821 aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag
    23881 cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa
    23941 gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta
    24001 catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa
    24061 gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta
    24121 cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga
    24181 ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca
    24241 aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga
    24301 tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg
    24361 tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa
    24421 aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca
    24481 cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat
    24541 taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat
    24601 ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat
    24661 tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga
    24721 aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat
    24781 aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc
    24841 aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta
    24901 ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg
    24961 ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt
    25021 ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag
    25081 agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat
    25141 gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa
    25201 tgccccatac taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa
    25261 agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccagcag
    25321 ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc
    25381 cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat
    25441 ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc
    25501 tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc
    25561 gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata
    25621 acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa
    25681 atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg
    25741 aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag
    25801 atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa
    25861 ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa
    25921 ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc
    25981 gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag
    26041 gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga
    26101 aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact
    26161 gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag tacatatttt
    26221 ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct
    26281 ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat
    26341 aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt
    26401 gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag
    26461 ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct
    26521 gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc
    26581 atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta
    26641 taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga
    26701 ctgccgacac ggcgtgccat tacttcgatc tctaggaaat cttcatgtgt cagtccgtga
    26761 cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg
    26821 taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga
    26881 cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt
    26941 acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact
    27001 agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta
    27061 cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag
    27121 gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct
    27181 aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa
    27241 atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg
    27301 tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa
    27361 gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt
    27421 ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat
    27481 tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag
    27541 cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct
    27601 tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa
    27661 acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata atcttccact
    27721 gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat
    27781 attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa
    27841 tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg
    27901 taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga
    27961 atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc
    28021 gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt
    28081 gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct
    28141 tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat
    28201 gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt
    28261 ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc
    28321 tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga
    28381 tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg
    28441 gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg
    28501 taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt
    28561 tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc
    28621 aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac
    28681 ttgcccacgt ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt
    28741 tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt
    28801 tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta
    28861 tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact
    28921 ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc
    28981 tgaatttctg atcactggag cgtaacctgc atcttccagt gtttggatcc cgtcacttag
    29041 aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg
    29101 taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg
    29161 ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc
    29221 ggctcctttg tataaattca tattttctac aatattatac tctaaaatca aagagttgtt
    29281 ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg
    29341 aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg
    29401 atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt
    29461 gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt
    29521 caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta
    29581 cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta
    29641 gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg
    29701 catgttagtt tgaaagaccg tcccacagca gggaaggttc gaatcgatta tgatctgttc
    29761 atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa
    29821 aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac
    29881 gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc
    29941 gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct
    30001 aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa
    30061 ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc
    30121 ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa
    30181 gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat
    30241 gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa
    30301 gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat
    30361 tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga
    30421 aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag
    30481 gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag
    30541 agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt
    30601 gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc
    30661 ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga
    30721 aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc
    30781 aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga
    30841 tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt
    30901 ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa
    30961 aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa
    31021 cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa aaagagattc
    31081 tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga
    31141 atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag
    31201 aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa
    31261 acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt
    31321 atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga
    31381 gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga
    31441 agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc
    31501 tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag
    31561 aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat
    31621 tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga
    31681 gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa
    31741 acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc
    31801 cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa
    31861 gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag
    31921 caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt
    31981 tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt
    32041 tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg
    32101 gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt
    32161 aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt
    32221 atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg
    32281 cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt
    32341 tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag
    32401 aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca
    32461 gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac
    32521 caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca
    32581 cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg
    32641 aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg
    32701 cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca
    32761 aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca
    32821 tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg
    32881 atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg
    32941 atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag
    33001 ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt
    33061 atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca
    33121 cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat
    33181 acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat
    33241 aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc
    33301 gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca
    33361 ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa
    33421 tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag
    33481 ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag
    33541 aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac
    33601 ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct
    33661 gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat
    33721 acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg
    33781 acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat
    33841 gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa
    33901 tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg
    33961 gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct
    34021 gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct
    34081 ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg
    34141 gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt
    34201 caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta
    34261 aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca
    34321 attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa
    34381 cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc
    34441 acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta
    34501 aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga agacctttca
    34561 gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg
    34621 ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga
    34681 acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat
    34741 gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg
    34801 aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat
    34861 gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt
    34921 ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc
    34981 tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta
    35041 ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt
    35101 attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg
    35161 tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca
    35221 gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg
    35281 tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc
    35341 gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg
    35401 aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca
    35461 taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc
    35521 tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga
    35581 acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt
    35641 aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc
    35701 agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc
    35761 ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat
    35821 cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa
    35881 atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat
    35941 cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt
    36001 ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg
    36061 gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag
    36121 caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc
    36181 aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag
    36241 aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag
    36301 atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa
    36361 cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg
    36421 tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat
    36481 gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg
    36541 aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc
    36601 atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa
    36661 caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag
    36721 ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa
    36781 aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg
    36841 gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat
    36901 ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg
    36961 cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg
    37021 ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg
    37081 gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa
    37141 atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt
    37201 gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca
    37261 ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt
    37321 ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt
    37381 ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta
    37441 gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt
    37501 gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct
    37561 tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct
    37621 aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa
    37681 atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt
    37741 ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa
    37801 atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt
    37861 aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc
    37921 gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa
    37981 acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca
    38041 tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct
    38101 atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc
    38161 gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagctgc agcagcagaa
    38221 caattaggat ttgctgacaa attcacacac atctcaacag gtggtggcgc aagcttggaa
    38281 ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag
    38341 tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag
    38401 ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa
    38461 ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa
    38521 aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga
    38581 attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga
    38641 aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca
    38701 ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat
    38761 caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga
    38821 atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc
    38881 tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat
    38941 ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt
    39001 tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca
    39061 atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga
    39121 cggtgcttta gtcggtggcg caagcttgca agcagattca tttttgtcat tgttagacgc
    39181 tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa
    39241 attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac
    39301 aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc
    39361 caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt
    39421 caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt
    39481 atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa
    39541 tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg
    39601 gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg
    39661 cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca
    39721 caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact
    39781 caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc
    39841 tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag
    39901 caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt
    39961 ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc
    40021 ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag
    40081 ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag
    40141 tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat
    40201 tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg
    40261 taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta
    40321 tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag
    40381 aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct
    40441 ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac tggttctcta tcacgtactg
    40501 accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag
    40561 aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag
    40621 atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc
    40681 tctacttgct gaaatatcag caggtagaga gt
//
LOCUS       DASJPH010000012        38921 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJPH010000012 DASJPH010000000
VERSION     DASJPH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.012"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="U3995_000599"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="U3995_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1795947.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /locus_tag="U3995_000600"
     CDS             complement(653..1375)
                     /locus_tag="U3995_000600"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1795948.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /locus_tag="U3995_000601"
     CDS             complement(1511..2392)
                     /locus_tag="U3995_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1795949.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="U3995_000602"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="U3995_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1795950.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="U3995_000603"
     CDS             3632..4111
                     /locus_tag="U3995_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1795951.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="U3995_000604"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="U3995_000604"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1795952.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="U3995_000605"
     CDS             5412..5540
                     /locus_tag="U3995_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1795953.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="U3995_000606"
     CDS             5544..6089
                     /locus_tag="U3995_000606"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1795954.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /locus_tag="U3995_000607"
     CDS             complement(6543..8234)
                     /locus_tag="U3995_000607"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1795955.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="U3995_000608"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="U3995_000608"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1795956.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="U3995_000609"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="U3995_000609"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1795957.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="U3995_000610"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="U3995_000610"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1795958.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12515..13216
                     /locus_tag="U3995_000611"
     CDS             12515..13216
                     /locus_tag="U3995_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1795959.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13825..14841)
                     /gene="tsaD"
                     /locus_tag="U3995_000612"
     CDS             complement(13825..14841)
                     /gene="tsaD"
                     /locus_tag="U3995_000612"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1795960.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14838..15302)
                     /gene="rimI"
                     /locus_tag="U3995_000613"
     CDS             complement(14838..15302)
                     /gene="rimI"
                     /locus_tag="U3995_000613"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1795961.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15309..15851)
                     /gene="rimI"
                     /locus_tag="U3995_000614"
     CDS             complement(15309..15851)
                     /gene="rimI"
                     /locus_tag="U3995_000614"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1795962.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15835..16557)
                     /gene="tsaB"
                     /locus_tag="U3995_000615"
     CDS             complement(15835..16557)
                     /gene="tsaB"
                     /locus_tag="U3995_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1795963.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16748..17728)
                     /locus_tag="U3995_000616"
     CDS             complement(16748..17728)
                     /locus_tag="U3995_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1795964.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17752..19236)
                     /gene="galT"
                     /locus_tag="U3995_000617"
     CDS             complement(17752..19236)
                     /gene="galT"
                     /locus_tag="U3995_000617"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1795965.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19248..20237)
                     /gene="galE"
                     /locus_tag="U3995_000618"
     CDS             complement(19248..20237)
                     /gene="galE"
                     /locus_tag="U3995_000618"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1795966.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20485..20652
                     /locus_tag="U3995_000619"
     CDS             20485..20652
                     /locus_tag="U3995_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1795967.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20714..22525)
                     /locus_tag="U3995_000620"
     CDS             complement(20714..22525)
                     /locus_tag="U3995_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1795968.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22522..22887)
                     /locus_tag="U3995_000621"
     CDS             complement(22522..22887)
                     /locus_tag="U3995_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1795969.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23050..23445)
                     /locus_tag="U3995_000622"
     CDS             complement(23050..23445)
                     /locus_tag="U3995_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1795970.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23463..24425)
                     /locus_tag="U3995_000623"
     CDS             complement(23463..24425)
                     /locus_tag="U3995_000623"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1795971.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24425..24637)
                     /locus_tag="U3995_000624"
     CDS             complement(24425..24637)
                     /locus_tag="U3995_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1795972.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24658..25356)
                     /locus_tag="U3995_000625"
     CDS             complement(24658..25356)
                     /locus_tag="U3995_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1795973.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25376..25918)
                     /locus_tag="U3995_000626"
     CDS             complement(25376..25918)
                     /locus_tag="U3995_000626"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1795974.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26050..27054)
                     /locus_tag="U3995_000627"
     CDS             complement(26050..27054)
                     /locus_tag="U3995_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1795975.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27051..28040)
                     /locus_tag="U3995_000628"
     CDS             complement(27051..28040)
                     /locus_tag="U3995_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1795976.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28037..28843)
                     /locus_tag="U3995_000629"
     CDS             complement(28037..28843)
                     /locus_tag="U3995_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1795977.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            29009..29965
                     /locus_tag="U3995_000630"
     CDS             29009..29965
                     /locus_tag="U3995_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1795978.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(30042..30560)
                     /locus_tag="U3995_000631"
     CDS             complement(30042..30560)
                     /locus_tag="U3995_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1795979.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30648..30797)
                     /gene="rpmG"
                     /locus_tag="U3995_000632"
     CDS             complement(30648..30797)
                     /gene="rpmG"
                     /locus_tag="U3995_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1795980.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            31025..31471
                     /locus_tag="U3995_000633"
     CDS             31025..31471
                     /locus_tag="U3995_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1795981.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31666..33561)
                     /gene="asnB"
                     /locus_tag="U3995_000634"
     CDS             complement(31666..33561)
                     /gene="asnB"
                     /locus_tag="U3995_000634"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1795982.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33886..34860)
                     /gene="bsh"
                     /locus_tag="U3995_000635"
     CDS             complement(33886..34860)
                     /gene="bsh"
                     /locus_tag="U3995_000635"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1795983.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35194..35281)
                     /locus_tag="U3995_000636"
     tRNA            complement(35194..35281)
                     /locus_tag="U3995_000636"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35247..35249),aa:Ser,seq:cga)
     gene            complement(35426..35992)
                     /locus_tag="U3995_000637"
     CDS             complement(35426..35992)
                     /locus_tag="U3995_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1795984.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36248..37579
                     /locus_tag="U3995_000638"
     CDS             36248..37579
                     /locus_tag="U3995_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1795985.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37545..37895
                     /locus_tag="U3995_000639"
     CDS             37545..37895
                     /locus_tag="U3995_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795986.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(37987..38406)
                     /locus_tag="U3995_000640"
                     /pseudo
     CDS             complement(37987..38406)
                     /locus_tag="U3995_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(38407..38751)
                     /locus_tag="U3995_000641"
     CDS             complement(38407..38751)
                     /locus_tag="U3995_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1795987.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT        11291 a         8078 c         6756 g        12796 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa
    12241 atatttattc attgaaaaaa tttttttgaa taaattaaaa aaataaatta tattatcgtt
    12301 gttagcgttt actatatgta ttattgttgt tttttataca caaatcattc gcttcaaaac
    12361 aaattattca ataccattgt atatttttta cataataaca aattatattt gtaaagtatt
    12421 aaaacttgtt tctaatgttg gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa
    12481 taaatatagt gaacaataag gagaggactt tttaatgcgt gttaataaaa tcgattttca
    12541 cttttcaaaa ctcagatatc aacctgattt tgaacatttc tcggatgaag agttccaaca
    12601 gatcaaggaa catactgttc ttcgttctta taaaaaagga cagatccttt tcgacgatgg
    12661 tgatcccaga caacggttgt atatcttgac caaaggactc gttcgtttag aacgttatga
    12721 tgaaagtgca acttattact actatgacta tgtgaataca aataaccttt ttcctatgtc
    12781 aggaatgttt ggagaagaga cctacactta tacagctcaa gcgatgactg atattgaaac
    12841 attctacctt cctgtttcct actatgaaaa attagcacga aaaaatcccg aacagttgat
    12901 ctatatgata caaaaaattt ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg
    12961 attgacctct agcgcctttg accgggtaaa acaaaattta ttcattttga aagaagaatt
    13021 gggcgtacca acaaaagatg gtcatatcac cattccttat ccgatcacct taaaagaatt
    13081 agcagtgaat agcggcacga cacgtgaaac tgctggacaa gtaatcaaac aactacgtga
    13141 ttccgggctg ttagattatg acaaaaagcg ttttactttc tgtaagacag ctactactcc
    13201 agctatgaga gaataacgcg aacaaaaata agcagtgacc ctgataaata tcagggtcac
    13261 tgcttatttt cctccctaaa acttctgaaa gatccggcag ttttagatca ctggacaaga
    13321 ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc actgtttgca aaaaatactg
    13381 ttagattaac tagatgaagc gtcttgtcct catccgatct agattattcg atattcctcg
    13441 acaataaacg attactcagt gagggcaatc agaggaacgg ataatgataa ataagctgta
    13501 aaaaatagca aaataacaat aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa
    13561 cgtgtcacag ccctatttcg attaaacggt gtgtacctgt caactcttcg gtcacaagtc
    13621 ataatgttca taaacatgag gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa
    13681 acgacaggct tacgaactct tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa
    13741 gaacagccag gtcaaaaaca tgagaagccg tttttcctgg gctgttctta ttacttgatg
    13801 cagggcagct ttttcacaac ctctttattc agataaaacc aagcttggat cagcattcaa
    13861 ctgatagcta gcaaaatttc ttttttgcat ttccacatgt gctgctgctc caatcattgc
    13921 agcattatct ccacaaagtc gaagaggggg aatcactagt tctacctcag ggagtttagt
    13981 tgccaaagca tcctgcaatc cttcacgcaa cccttgattg gccgctacac ctccagctat
    14041 aatcagttgc ttgactggat aattttgaca agctcgaagt gttttagtaa tcaatacatc
    14101 gatcacactt gcttgaaagc tagctgctaa atcatttttg tctagatctt ctccacgctg
    14161 ttgtgcatta tgcaccaaat tgataaatgc actttttaat ccgctaaaac tgaaatcata
    14221 attatcttca tggatcatcg ctcgcggaaa atggtaatta tcttttccct gatgagctag
    14281 ctgatcgatt tcttttccgc ttgggtaaga aagacctaat actcttccaa ctttgtcata
    14341 agcttctcct gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc catcttcttg
    14401 catatagact aattctgtat gtcctccgct aacaagaagt gccatcaatg gaaactggaa
    14461 tggtttaacc agacgggctg catagatatg ccctgccata tggtttacag gaataagcgg
    14521 caattggtgt gcccaagcaa aagccttagc tgcagaaata ccaatcagca acgagccgac
    14581 aagtcctggt ccataagtga cagcgactgc cgataaatct tctgccgaca caccagcttc
    14641 taccagtgca tcttccaagc ataaggtgat ttgttctacg tgatggcggc tagctacttc
    14701 aggtactacg ccaccaaaac gtttatgact gtttatttga gaagcaacga tgttagataa
    14761 gatctctgtt ccatttctaa cgaccgctac actcgtttca tcacaacttg attcaatcgc
    14821 cagaatcaat tcttcattca tttcatcgtc tccgttttta actttctgct cataatgatc
    14881 gcatcttccg tcggattgcg ataatatgat ttccgtttac ctaatatacg aaacttttct
    14941 gattcataaa gttttcttgc tgcttcatta gatagacgta cttctaaaaa aacatgcagc
    15001 gaatctttta tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg cccttttcct
    15061 tgttctgaaa ctgcaacagc aatatttgtg atctcgactt catctattac tttcgagtat
    15121 ccaataaagc cacagatctg gttatcttca gctaaaagca gataatcaga gtgaggctgt
    15181 tggatgtctg ccaaaaactg cattttcgtc caaggtgaac catgtggata agcattttcg
    15241 ctaatcatcc atacgcattc tgccaagtac gcttcatcaa aatctttttt tctgcaaatc
    15301 aactaatctc actcattttc taaaagtttt atcatatcca acgcatcttc ttttgtttca
    15361 gtgtaatatc tttttttgat cgttgttgct tgaaacccca atttccgata aagtctttgt
    15421 gcatcttgat tactcatacg cacttctaat gacatcgtct cacagcgatt cattatagca
    15481 aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata tgccggtcga
    15541 acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc
    15601 attcttccat tatcaaagat acctagatat agatgaggaa gcggtgactt caattctgat
    15661 ataaaggcac tttttgtcca tggcaattcg ccattataga cttctcgttc gacatccagc
    15721 agctctttga tatcttctgt agtcaattct cttagtacgt aacgacgatc gatttgttcg
    15781 actgtttttt ctgtataaat cattttatct tgaaaaaagc ttctggcata cgctctaaat
    15841 tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc
    15901 tactcgtttt aaatagcgag gaagaaaagc ttgtttctct gcaactggtt caactaaagc
    15961 acctaattga gccacagtta caccacttgg aatatcccat gctggaactg tattaatcat
    16021 cgcactaggc aatatttcct ttatttcaga gaagaatttg tgacaatcac tcccgacgaa
    16081 ataaatagct tcattcagct tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac
    16141 atcttcaagt acagtttcta atctattttc tttccagcgg tatgccccag cgtatacatt
    16201 ttttcttcga gcatcaaaca tgggcacaat aatattatcc acaccgatgc aattagccgc
    16261 gatggttttc agactagata ctcctactag ctctttatct aatgtataag caatcgtttt
    16321 tgctgtcgtt acacctatac gaagccctgt ataagaaccg ggaccatcag aaaccgcgat
    16381 tcggtcaatg tcttttggac ttatttgaag atctgcaaac aattggtcga ttgcaggcat
    16441 cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc
    16501 ttctaccaca ccgatcgcca aagtttggtt tgcagtatct attcctaatg taatcatata
    16561 tttgatcctt tctgctatga cattctcgcc ttccattgta gcatactttt ttattctgag
    16621 gatgaaaata ccaaaggcaa aactagtaca ataatacatg attatctgct ggcgggcaaa
    16681 ttttgcccct tcctgaaaca caaaaaagct gcggtatcac tccattttcc cacagctttt
    16741 ccatctttta tttcgttgat tctcgaataa tcaactctgt ttccacaata atctttcttg
    16801 aaaccggtgc tttggaatta cttaattgaa gaatcgtctc cactgctagc tctcccatcc
    16861 attcggtata aacttttaca gtagaaagcg agggcgttac atatttcgct acactgacat
    16921 cattgaagcc catcacagaa atatcttctg gtaccactaa acctttttct tgaatcgctt
    16981 tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag cgcatcagga acatgcggat
    17041 tcgtttttaa atactgcttc actgcttgat atcctgactc gacagaaaaa tctgcttcga
    17101 taattggacc ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc ataattctgg
    17161 gatcttttag ccgctgatgg tttttcttcg tatattctgt tccagcaagc atagcgatat
    17221 gttgcgcttt atgggagaca aagtgttcaa tgactttttg aacacttgac ttaaagtcga
    17281 caactagaga gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt
    17341 gggatccttt catttctaat aactgagctt cgtcgaattt tcctagtgcg atcgttccat
    17401 ctgccggaag atcactaatg gaatcccacg tttctttcaa taaagtaacg tttgcttctt
    17461 ctgccttttt ttcgattcct aggcgaatag ataaataata aagatcttca agctcttccg
    17521 tatcgtcgta ccactggatc aggcgaagcg tttgcttagt atttgctgca tttttacgat
    17581 gtttcgtata atttaagcgt tctgctgctt caaaaatttt ttgctttgtg gctaagccaa
    17641 cggaaagatc aggatcatag ttgagaactc ttgaaacagt agcaggagaa actcctgcca
    17701 actgcgcaat gtctttaatt gttgccattt gtctctcctc cttttttacg tttataaaat
    17761 cgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc
    17821 ttccagtaca cgtgcaaaaa cctgacctac agatttttgt acgacatcgg tgaccgtatc
    17881 tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc cacggacgat gatattcagc
    17941 catctggttt tcttctccta ataagaaatg ctctacttct actagttctg gtttcaaacg
    18001 aggtggtaaa acagcaagcc ccattacttc gatcaaccca atgttctcct ttttgatatg
    18061 ttggacatct ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc
    18121 tcgaaggaca aggtccaact caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt
    18181 atggtgtggt gtaccatctg ctgaaaaagc tctgatagat actgtttcat ccgaatatgc
    18241 tttccatttt tccaatactg agttagctgc ttcaataagc aagtttttct ctgttgattg
    18301 caaacggatc acagacatcg gccatttcac gactcctgcg atgacttgcg gataatcttc
    18361 tagtttgata agtttttcta tcggtgcttt tgccatagga aattcatgac gtcctgcctg
    18421 ataatgatca tgagaaagaa tcgatcctcc aacaattggt aagtcagcat tcgaaccaac
    18481 aaagtaatga ggcaagactt cagtaatccg aagcagtcta gcaaatgttt cctttgtgat
    18541 tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat aagcataagg
    18601 ggaatattgg aatccccagc tttcaccgtc tagattcatt cgaataatcc ggtgattggt
    18661 gcgggctgga tagttcaagc gacctctata tccttcgttt tccatgcaaa gcatgcaggt
    18721 aggatagtct acttttaccg cctcgcgttc tgctgcgatt tgttttggat ctttttctgg
    18781 cttagataaa ttgattgtga tttccagttc accatactcc gactctgccg gaaatttgat
    18841 gttttttgcg atggctcttg ttttgatgta gtcatttttc ttacttaaat taaagaaata
    18901 ctcagttgct tcctctgggc ttttcgaata gtgctgtgca aagaatgcat tgacgactga
    18961 aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa
    19021 ttctgaaaga acaccatttt ctcttgcctt agcgacaagc tgatccaata agtctacaga
    19081 ttcttcctta actggtctta cttcaatgct ttctagagca tcttctccta ccatccctaa
    19141 aagacgattc tgtaaatata agcggtccat ttccatccat ccgccagctt cgatcgcaag
    19201 tgtggcaaaa tcacaaatcg tttgactgat tgacatatct cattccccta ttcttcataa
    19261 ccatttggat ggctgacatg ccaatcccat gctgttttga taatcgcttt gatatctgtg
    19321 tattcaggtt tccagcccag aatctctctc gctttttcac ttgaagcgac taatcggcta
    19381 ggatctccag ctcttcttgg tgctatttct gctggaattt cttttcctgt cacttctcta
    19441 gctgcttcta gcatttcctt gactgagtat cctttgctgc tgcctaaatt gaagaagttg
    19501 ctttcatttc cttctttcaa gtattccaaa gctaaaatat gtgcagcaat caaatcttct
    19561 acttgaacat aatctctgat acatgttcca tctggtgtat cataatcgtc tccataaaca
    19621 gccaaggctt ttctttgacc aagagctact tgtaaaatga ttgggacaag atgcgtttct
    19681 ggtgtatgat cttctccgat agatgcatct gcttttgctc cggctacatt gaagtagcgc
    19741 aaagcgacat agcgcatgcc atatgcttgg tcacaccact tcatcatttt ttccatcatc
    19801 aatttacttt caccatacgg atttttagga ttagctggtg tatcttctgt gattggagac
    19861 tctttcggct ctccataagt tgctgcagta gaagagaata cgattttatt cacgttgaac
    19921 tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa atacatcaat
    19981 ggtttttcga cagattctcc tactaaagag cttgcagcaa aatggattac tccttcaatc
    20041 ggttcctttt caaatacgga acgtagaaat tctttatccc ggatgtctcc ttcataaaaa
    20101 tgagcatctg gatgtactgc ttgtttgtga cctgtgagca aattatctac cacagctact
    20161 tgatacccct tttgaactag ctggtctaca gcgtgcgaac cgatataacc ggctccgcct
    20221 agtactaaaa ttgtcataaa aaacactcct ttgattagta aaattgtgtt tgttgattct
    20281 atataattta aaaaacgaag tttactttta tggtgaaaac aaacaaagta aaatttcgta
    20341 aattcatatg gtaaatttat aataaaacat ttagtaaaca ttttcaagga ttttttgcaa
    20401 aggtttacaa acaaacgtaa aactagcctt tttttctctt ttgttccata atagatataa
    20461 caaataataa atgaggtgga ttcaatgaaa aaatttgtat ctggaatatt agtaggaagt
    20521 ctggcaacag ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat
    20581 ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc
    20641 gtttctcgtt aataattatt taaaaatacg catgaagact ttctaaaaaa cagataagtc
    20701 atcatgcgta tttttatttg tcagtcaata taagttgtgc tattttatct gcctgtctca
    20761 gattccatgg atctgtataa ggtctagcta ctgctgcagt tagccaatcc acaccttcaa
    20821 caggtattcc atagcaataa aagtcagact ggatttcccc tgaagcatca atcacgcgat
    20881 tcgtacctcg ctcaacatct atagcttctg aatgataact tcctgtatta tctgaaaatg
    20941 aaaaagggcg aacaagtttg ttttccttca aattttttat gacaggatta cttgaacgag
    21001 aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt tctttatctt
    21061 gtagagaaat aacatagcgg ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat
    21121 gaaaaatacc cgcatctatc agtgctgcta attctttcgt ccgaatgatt ggcggaccga
    21181 tcgttaaaaa gctatttaga tgagtaaacc atccccatag tatttctttg tattccttag
    21241 cagaaaaaac gtgccattca atagcctgcc ggattggatc acgccagtct tttaatgcat
    21301 caaatgttga agcaaatgct cctgtgcaat tgcctttttc cgcttctttt atctgccctt
    21361 caataaatat tcggctagct tctgaaaagg gctcattctg ataggcaaga ggagtttcta
    21421 aatacgacca atcccatcta taagggatta gtgctggatg ctttccgaga cactcctctg
    21481 aagagaggga gagaaaatca tgctcaaaat ttttctttgg aatcggcaag tgatattctt
    21541 cgatgatttt cttgtaataa aacaactcaa cgtctttttt cagatagtca aaaaatgttt
    21601 cgccagtcaa aaatcccgac tgatgccatt gctccaggtt ttcatttgtt agtaaccgtg
    21661 gccatgccat tgctccgcct tgcttttgat ttcttcctcg agggtaataa gggagccccc
    21721 ttcttgatcc actgtataca atcggttcct tgcctgatgg atgatagacc agttttccct
    21781 tttcgttctc gaagcgccct cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac
    21841 ttaagcctag acctcttaaa aagacatttt ctttaggcgg tatcttcgaa agatctacat
    21901 ctgcaggatt agaaggaagc tgatacagta aaacattttt ctttgcgtag tctacccaca
    21961 ctctttcttc ttctgtctca tcattttccc agtgaccgct tgctaaaaca agctgatcga
    22021 tagaatagct ttgctgatct gtctgtaaaa gataaccatc ttttcttttg cttacttttt
    22081 ttactaaaga ataaacaaac tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa
    22141 accagcgttg atagagtccg tataaacatc gagtagattg ctgatctttt tccagacgat
    22201 tgatttcttc tataattagt tgcttatttt tggttaccgt ctgttcgata taatctgtag
    22261 caggtccttt actccattga aacaagttgg gtccctgtgt taccaatcct ccactggaca
    22321 atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga attcattaat aactccggtg
    22381 gctgattcaa ccgccaaact cgacctccag gaccattggg atcgaaaaga aaaagttcaa
    22441 gagaatgtct gtcctgatta ttatggagaa tcctttctgc tacacttaat cctctaggtc
    22501 cggcaccaat gatccctatt ttcatttgca tgcctccatt tcgataaatt ttttcaataa
    22561 aacagcatgg ttctgatgtt cattttttgc accataaaca aaagtaatga ctggatgttc
    22621 tttcataagt tgactgagtt taaaaatagc ttgcttcttt tcttcttcga aaatcaattc
    22681 ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa aagttttgcg
    22741 tgcttcatta ctaggtgcaa tttctttcaa ccacagatct aactgcgctt tttcttttga
    22801 aattcctcgt ggccagaatc tatccacgag tattctatag ccatcctctt tttcagcatc
    22861 aaggtaaaca cgttttaatt tgatcatagt tgtttccttc tttctacctt ttcgcttagc
    22921 ttatatatgt agataatagc acaaaataaa aaaaatgaac ggcacaacta tccattgcgc
    22981 cgttcatgtg atcttggtca aaatctaatg attgattcgg tttttaaagc tgtcatacaa
    23041 tcatctgttt tatacttttt tacttttgat ttttccagac catccgtcat atccaccctt
    23101 aagtaaataa atctcttgat aacctgcttt acgtaattta tttgctgtac ggatgctcaa
    23161 acttttcttt tgatcgtaaa tatagacagg ctggtctttt cgaatagatc ctaatgaatc
    23221 tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata tgcccagcat caaacacatc
    23281 tttttcgcga acatcgatta cttgtgcttt tctcattcct tctcgaaatt cttcttctgt
    23341 caaagtcgtt gctgaacgtt tggccattac tcgtagatag acttcccaaa ggatgattgc
    23401 aagcacaatc aatagcaaaa tgatattaat tacccataaa accatgtaat gcctccgttt
    23461 ttttaagcaa atttcaaggc taatgaggca gcaccaatga caccagcttc attaccaagc
    23521 tctgctaatt tgatttttgt tgattctctt acttgcggga aagtaaattc tttaaaatat
    23581 ttctctacac ggctacgtaa aaattctcct gctgccgata ctcctccacc taacacgata
    23641 cttgacggat ttaatgtatt ccccaagttt ccgcaagcta aacctaagta gaagcatacg
    23701 cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa gatatcttta
    23761 cttgttactt cttctccgtt gtctagcatt gctttcagtt ttgaatcccc tgcgtattct
    23821 tctgccaaga aacgacctag gcgtaccaca cctgtagcgc tagatacagt ttctaaacat
    23881 ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg tccgatttct
    23941 cctccagctc cgctcacgcc gtgaatcaaa tttccgccag caacgatacc tccgccaaca
    24001 cctgttccta gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc tttccatcgt
    24061 tcacctaaag cagcaacatt cgcgtcatta tcgatagcga atttgattcc tgttcctgcc
    24121 tcgatcagtt ttttcacaaa ttgaacttct gtccagttta agttatacgc accaataacc
    24181 gttccagctt cgctgtccac gcttcctggg gtacccatgc cgattccgat aaagtcttcg
    24241 gctttcatat tatataaatt caaacgatga ttgattgatt caatgatttc aggaacgata
    24301 tgcttaccat catccaaaat attggtatcg atgctccatt tttgctgaat ctctccttct
    24361 ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat caatcccaat aatcttcttg
    24421 tccattacaa atttcctttc tttcggcttt cttcaattcg gtgctctctt tttaatatgc
    24481 gccacgctgt catgtatgta tcatgatcaa tcaaacggct atcatacaga ttacgcaatt
    24541 cgatagtcat taattcaatg tcatagatgc gtgcacccac atagataaag atgccaaatt
    24601 ttttgaacag ctgctgtaca tcatataagc tctccatctt taagcacttc cttcatatta
    24661 tacaagtaaa ccatattttt ttaaacccag taaaatacat attacaaaaa gaaatacaaa
    24721 aactagtgta gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt tcggtagacc
    24781 caaaacattc cctaacacta atccgccaat tagcccaccg acatgtcccc aaacatctac
    24841 agaggtatcc agaagaccaa aaacaaatgt caacacaata aataaaagaa aatgtctgac
    24901 tagctgctgg atcgctgggt tatgtttaaa atgaaaccct aagataaaca acgcaccgaa
    24961 caaaccgaat atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa
    25021 gcttgctgta tttcccatta tcccgctgaa catataaatc aagaaaaaac gccaatggcc
    25081 atagattgcc tcgatttgct gccccataaa atacagtatc agtgagttca ttacgaaatg
    25141 catcagtcca aaatggatga agattggtgt cactaaacgc caccattcat tgaagtgaac
    25201 aagataagga ccatacattc cggcataata agggatctca agtcgcggaa ataattccat
    25261 catgaggaag accgctgtct gtatccctaa aaaagcatag gtccataacg gtcgtcttaa
    25321 ccatacacgc aattttattt gctgttgata attcatgaaa acctcccatt tcattttagt
    25381 caataaacaa ttgttggatc ttctgatcaa agctctccgg ttgccattgt tcattcaatt
    25441 gttcacgaaa aacaaggcta catgtttttt tatgaaaatt atttaaatac cggtcgtaat
    25501 aaccaccgcc aaaaccgatt cgataaccgg ctctattgta gataatacct ggtacaatga
    25561 ggagatcgat catctccggc aaatagagct tctctgattg tggctcaaga acaccaaaac
    25621 tcgaacgtac aagaacggac tgttcatcca tttcataaaa cgccatctga cgtttcggca
    25681 aagttttagg tacaactatt ttcttttgtt ctttttctgc tcgttggata accggctgtg
    25741 tatccagttc aaattcattc gataaagtcg tccctatcac ttttgctgac ttccagtact
    25801 cgctggcgaa tagctgttta tagattcgct gttctttttc ctttttcttg ccacttttgg
    25861 ccagattttt cagcttttcg attcctttcc ggcgtaatct tactttcaat tggtccaaaa
    25921 actcaccctt ccttctctca taaaatggta taggaaaaaa agaaaaatag caatcgaact
    25981 ttcaaataaa tgcataaata gaataaaaag aagacctaga aatgattggc cttccttttt
    26041 taatgaattt caactgttca gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata
    26101 tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact tccccactta gtagaatcac
    26161 tctagcaacc atatctgcac taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa
    26221 ggtccagtta ttttctttac gtacgactag cttcgcagca tgaggagcta gtagtcctac
    26281 aaaagataaa ctacctgtca ccgctacact accgcttgcc aaagcaacag ctaacaacaa
    26341 aaggattttt tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa
    26401 tgatagcaat acaatcctct gtgcataaag tcctcctagc ggaattaaga taaccgccca
    26461 tggaagcaat gcccaaacat aaggccagct cgcaccccag attgatcccg ctaaccatgc
    26521 attcacaaat ccataatttt ccttagataa ttggatcgtg catagcaatg ttaatgcaga
    26581 aaggccggca ttaacggcaa taccggacaa taagattcga ttcataccca aaaagccatt
    26641 tcgatcatag gccgcaagat agacacagca agcagctaaa acaccaccta gaaaggctac
    26701 aactagcaat agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa
    26761 aaatcctgct ccggcattaa tgcccaatat actagaatct gccaattcat ttcttgttac
    26821 tccttgcaag atataaccag aaacgccaag acaagctcct gctaataaac tgatcagtaa
    26881 tcgaggaact cgaaattctc taacaatcag cgccaaagaa gatgattctt tccctatgaa
    26941 cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca atcattaaac tacctataga
    27001 caggatgaca agtacaaaaa gcaagaaata gaacgtccaa tatatttttt tcaccgttgt
    27061 tccctcctta cttgtatcag taaaaacgga acgccaatca atgcaatgac taatccaaaa
    27121 ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat ccgccaacat aaccaatcca
    27181 ccgccaacta aaaaagagac aggtagaaca taacgatagt ccgttccaac cattccgcga
    27241 acaactggcg gaaccaacag cccaataaat gaaacaggtc cgactaacga aacggcaatg
    27301 gctgctaaca ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg atgacttcca
    27361 agagctaatg catgtgtttc actcaaatga atcaaagata ttgcttttcc taatgaaaaa
    27421 gaaaccaaaa cagcaagtat atataaagga aacaatactg ctaattgttt ccatgtgata
    27481 ttggcagctc cgcctacaaa ccaaaaagca agatcctgct gcagattaaa gaattgggta
    27541 agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt
    27601 aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa agttacagca
    27661 aaagaacctg ccaatgacat tagaaacaca gaaaaatcag aagctttagg ccaaaaagca
    27721 aaactaaggg ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct
    27781 aaaggatttc ttgtcactcc ttgcatcaat gcaccgctca atgcaaaaca actccctaca
    27841 agaaatgctc ccgccacacg aggcaaacga atatttcgta tgatttggtg actttgtttt
    27901 gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt ttcccacagt cgctgcgcca
    27961 tttgaaagag caacaaaaaa aataatcaaa agaacaaaac agaccaaaag caggagtcta
    28021 tttttcttca tagtcctcat aattgatcct cctgctcttt tggcaacaaa tcataggtta
    28081 gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt
    28141 ttttcataca ctcttttgtg atcacttctt caggtgtacc ttctgtgatc aacttaccgt
    28201 ttttcattgc taataaataa tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca
    28261 tcaagatcgt tgtttgtttc atttgattga tttcagtcaa taactgtagg atctctaatt
    28321 ggtgtgccgg atctaaaaat gttgtcggtt catccaagat caagatatct gtttcctgag
    28381 caagcgccat agcgatccag acacgctggg tttgtccacc agacagcgtt gcgatatccc
    28441 gcatacgaaa tccagctaga cctgtagcat ccaacgccca ctcgattgct tcatgatctt
    28501 gcgtgtccat gccactgaac agtttttgat aagggtatcg tccgtaagat actacttctt
    28561 ctactgtcat acctgtaggg tgttcactag tttgtgctaa taatgcaatt ttttttgcaa
    28621 gttcttttga actgtatgta gagatatcag agttattgac aatgatcttt cctgatttca
    28681 caggtagtat acgggtaaaa cttttcaata aagtagattt tccacttcca tttggcccaa
    28741 tcaaactagt gattttccct tcaggaatcg taacagacat gttttcaatg atttttttgt
    28801 tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt catttcaccc atcctttcgc
    28861 tgaaaatcat tctcaattat gacgctgttt caacatttcg tcaagcgcta ctttcatatc
    28921 tatagccaaa tgaagcggtt gacagcgttc gaaatatcag gaataataac caatgagaaa
    28981 gattctcaat tatgttagga ggaaagaaat gaaattaaaa acaaaagtat tcacattatc
    29041 tagcatattg ctgctggctg gagtactgag cgcttgctcc ccctcttcag ataaagaaac
    29101 agctacaact gccaattcga cgacttcatc caatagtact catacagtca cagatacact
    29161 tggacataaa gtagagattc caaacgcgcc caaacggatc atcggcagtt acttagaaga
    29221 ctatctgatt gcactagggg aaaaacctat cgcccagtgg actgttggca gtggttccat
    29281 tcaagattat ctgcaagatg atttaaagga tgtcccaacg atttcttatg accttcctta
    29341 tgagaaagtg ttaagctttg agcctgattt gctgttgatt tcttcttccg ctactgtcga
    29401 aggcgggaaa tacgaacaat atagcaaaat cgcgccaact tatgttgtaa aaaacggaac
    29461 agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga
    29521 ggcagaaaag atcatttccg attatcaaaa atccatcaag aaaactcgag aagaactagc
    29581 agataaaatc gagaacaaga cagctgcagt gttgtgggtg accaacaact cagcctttat
    29641 ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac gatctggaat ttggtgttcc
    29701 tgatctagtg gaagaggtct ccaaaaaagc cacttctgac tggtctgctg tttcctctga
    29761 aaagctagct gaattagatg ctgactacat catccttgtg aacagtgaca aagatgctgc
    29821 catgttcaat gaaccaagct ggaagaatct taaagcagtg acagacaatc atgtactgca
    29881 attcggacct gaatctagtt ggctatataa cggacctatt gccagtcagg aaatggtcaa
    29941 tgatatcaaa gaaaaactaa attgatttta actaaaaaaa gcatcttcac gattagtcgt
    30001 gatatgcttt ttttcttatt aacaaactag acgcatagat tttattcttc ttttgaagca
    30061 accctatttt ttactctttc ccatttttga aaacgatggg catatagatt ttcttgatcc
    30121 gttatcccct catactcctc tataagggcg aagtctgaaa aatcaatatt cccaatatac
    30181 gtatcgcctt caaacgtgct atcgatcaat gttcgccaga tccgaccagt gtaaggaaga
    30241 agttcttgaa aaatccgact accacccgaa acatagatat cttctgagat cgtttgacta
    30301 tacgacaaaa gttcatcgat cgaatgcatt acttccgcat tttctttcgt gcgaaaattt
    30361 ggctgcgttg tcaacacgat gatatggcga taaggaagtg aaagatcccc cattccttca
    30421 taggtctttc ttcccatcac taaaattcga tccattgtat gttcgcgaaa gaaacgcata
    30481 tcatttggca atcgccatgg taacaaacca tttttgccga tcagaccatt tttatcttgt
    30541 gcccacatgg aaataaacat attatccgct ccttatgatt ttagatgttt tgagataaaa
    30601 aaagtctcaa ttctttgacc agcaaggaat tgagaacttt aaaaacatta ttttgtttca
    30661 cggtgcaaag taactttgcg ttcgcgtggg caatattttt gtttttccaa acgatcagga
    30721 ttgttacgtt tgtttttgct tgttagatag ttacgttctt tacaagaagt acactctaaa
    30781 gtgatgttta cgcgcatgtt tttccctccc atttttacaa gaatcctcta agatgactct
    30841 cagacttgat tatcttacca tgattttttc tagattgcta gtgtttttca cgattttgtt
    30901 aagtaaaact acttgacgat aaatacaacg gttttacttc tcaaacaaaa tctttttctt
    30961 tttgtataaa tacccttaca cttgtttata tagattatgt tttttaataa agggggatac
    31021 taatatgcca tggaacatga aggactttcc tgcatcaatg aaaaacttag ataaattgac
    31081 aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat gaagggtacc ctgataaccg
    31141 tgcgatccca attgccattg atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga
    31201 aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc
    31261 tcgtgctgga aaactgcttg attcagatgt gatcgtagaa tatgaggaag aacaatggat
    31321 cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa aagaagcaat
    31381 cgaaaaagga aagcaagtag cacaaaacaa agaatcaact ttgttcattt ataaaaaaga
    31441 tgggacaaaa gaaaaagaaa tcgattattg aacatatgta aaggggttgt gacgaaaatc
    31501 gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc gaaccataag
    31561 tcataatatc tataaaacgg gaagagctgt tttttgaaat tgtttcttat cactcagtgt
    31621 taaacggtag gagaaaacct cttttttatt gatatttgat tttcatcaaa cagtcacata
    31681 gtcgattgct tctgcttgcg gaatcgtttc atctacaaag tagactttat accatgtcaa
    31741 gaaactgaag attgtccaga tttttctttg ctcttcttta cgtccttcgt gatggtcatc
    31801 tagcaacttc atgatttttt cttgatcaaa aaattctttt gcaaaatctg cttcgaacaa
    31861 tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg gcacagggaa
    31921 tcctaatttc acacgattcg accatttttc cggaagatgt ttgttcgcag cttgtctgaa
    31981 aatatctttt gtgttgtttt gattcaataa aaacttactt ggtatttgtt gagccaattt
    32041 catgacttcg atatccaata aaggtactcg gacttccaaa gaacttgcca tagaaagttt
    32101 atctgccttt aacaagatat cttttggcat ccattgatga agatcgacat attgcatctt
    32161 attgacttca tctttgattc cttctgtttt ctcgtaatga acagtcatga tttcatccac
    32221 agatggtgct gtttgatatt ttggtgttaa cacttctgat gcttctgatt cttcgaacac
    32281 tcgagcatga ccgataaaat attcttttgc aggagcaagt gattcatata aatggatccg
    32341 tccatggaaa tttttcattt ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg
    32401 taattttttc aatccttgag caatcacacg gatgaatttt gaattcgtat ggaatccata
    32461 cgtttggtat cccgcaaaca gttcatccgc cccttctcca gattggacta cccgcacact
    32521 ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc
    32581 atctaagtga tactggatca aagggaatgc tttgaacgac atgtctccgt cgatcactgc
    32641 tgctgtatta tctaagtcca atagttcggt tagttttctg gcttcaatcg cttcattata
    32701 ggttttatca tcaaacccga ttgagaaaga atgatctggt ttcaatacag aggttacata
    32761 actggaatca accccgctgg ataagaaaga accaacttct acatcactga ccgtatgtgc
    32821 ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt
    32881 ttcttttgtt tcaaaatctg catcccaata ttgctgaatg tctatttttc catctttata
    32941 cgtataataa tggccttcag gcaaacgata gacatctttg aagaatgttt cgccgtttaa
    33001 tggagaatat tggaaagtca tataaggttt caacgcttct ttgttcaatt ctttgttaaa
    33061 atcaggatga ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc cgttcatttc
    33121 tgcataataa tatggtttga tcccaaagtg gtctcttgca ccgaataact cattcttttc
    33181 attatcccaa atagcaaaag caaacattcc gcggatcttt tgcaataatt ctgttcccca
    33241 ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc
    33301 tgcaatcaat tcttctctca aaggctggta attatagatt tcgccattaa aaataatgat
    33361 ctttgtccga tcttcattgt aaataggctg agtaccgccc tctaagtcaa taatacttaa
    33421 tcttctgaat cctaaagcaa catgtttatc gatatattct cctgaactat taggtccacg
    33481 gtgaacgatt cgatccatca ttgtattaat aattgttttt ttgttgtcct tgtcgttaac
    33541 aaaacctaca ataccgcaca tttaactttc acatcctaat ataaaattac tgtttatttc
    33601 attcatatgc atcttatcat agaataaata gcggacgaat attttctctc ttacaatttc
    33661 gtcacgtgaa aacaaaagat gaaagtttct gtttttacag aaaataaagt cccaatatct
    33721 aatttaccat gttttctttt ttgaggctac cttattctct tcgtgttaaa aaaatattag
    33781 tggatgctat ttccaaaaaa accagcaaaa aaagacacga cctaaagatc gtgtctaatt
    33841 atgactgtat cagaaaatat gataccaaac ggatctattc attaattaat tgacatagtt
    33901 tatctgttgc tcttttatga ctggataact gaccaattca tgacgatcca agtcttcttt
    33961 attcatattt acagcagtaa tttgactgtc ttcatacgta cgataataat aaatcccttt
    34021 atcaacgtta cagcaagaag aataaatcgt atattcgtat tttctttctc cagtatcgca
    34081 tactcctttt tgttgctcga ctgagcctag tatatgaaaa aattgactga tactttcaga
    34141 ttctgaatca cctgatgcag cattcatttt agtaaatgtg gcttttacaa atcgagaaac
    34201 agaagagaga tctccaggta agcccaatcc tcccatccca cgactataaa cattcaagct
    34261 gatctggttt gaaaaatgat tttctggtgt tccatttgat agcgagcgat aattgttcag
    34321 attaaataat tgataatcaa atggcggatt atttgtcaac acaccgacag gattatcata
    34381 taaatgaagt ccatctttca tactttcaat tactatcgat ttttctttat cagctaataa
    34441 ccagtgtaaa ggtgacaagg gtagtttttc actaaaatcg atattgacta gattgatagt
    34501 agccaataat tctttagctt cgtctatggt tgagcattgc cctagtatcc atggaataaa
    34561 ctcaaatggt gcaacgttat cttttccttc ttgtaattct ttataatcag cattgccagg
    34621 aaaattcagt cctgccatac tcaacccttt ttcatttgta gcctcataat agagtggata
    34681 gttggatacc ccagctgcta ttcctatcat tgcataatgt ttatctaaat tttttacctt
    34741 tctaaaatcg aaacgataat ttcttggagt aatagtgacc acttcattat aagacatctc
    34801 ataatcaaaa ttccttccaa aataatggtc tttcgttgca taagtgattg cggtacacat
    34861 cgtttcccct cctaaaacaa atcgttttta cacttttatc ttacatctag tatttttttt
    34921 atcaaaaaaa ggccaaagaa gattgattta ctagcattta taaaaaatag tactcagggg
    34981 gaatcaaaaa atattgtagg ttaaaagtac aacaataagg ggctgtgaca aaagtcttgt
    35041 cacagcccct tattccgaac aaacggtgtt caaaagcttg catctgcggt aataagccca
    35101 atcaaacgaa aaaataggaa aagctatttt tgtttggccg ttcttattac ttgatgcagg
    35161 acagcttctt cacaacctct aatctttgat ataaggaagg tacaggattt gaacctgcgc
    35221 gccggtatta gccggttcgc cggatttcga gtccggtgca ttaccactct gccaaccttc
    35281 cgtaagtact atttttaaac ttttttcacg aaatgtcaag cctatttcta atagataaat
    35341 aacagaaatc gcttttttta gcaaaaaagt ccattgacag tttgcagcca acggacttac
    35401 cgatcaaatt catactgata gaacactatc tgattatttc aggattatct aatgctaaaa
    35461 tcgagtcaat tgtccgtttg atcagtgcaa tgatttgttc ttctgttgga tgcatgatgt
    35521 ttgatgacat caatgacgct aagccagtca ctgctaccca tgtaccagta tgcagagcat
    35581 cgacatattc atccgataat tgattgtatc tttcatcttc tttaactatt tcacggaaat
    35641 aattgtatga aaaatcatgc attttctttc cgtccccatg ttcatcaatg tacaatgcca
    35701 tgtaaagact atgatgcttt tttgcaaagt agatatagtt caaaccgaga tcaatgattt
    35761 tatcaccagt acgttcaata gggaaaacat cttcttttag ttgttcaaag atagaatcca
    35821 gcaacgtatt ttttaaatct tccatgtttt tgaattccag ataaatcggc tgtgtcgaaa
    35881 tccccatctt tttagcaata tttctcgcag taaatccttt aaacccttcc tttgcaacaa
    35941 catcataagc agcttttaga atttgatcct tggtatagac ttttcttctc attaaaactc
    36001 ccccacttct ttactgcagt atatgttgtt ttattgtacc ctatttttag cattttttgc
    36061 aaacatttta ctcaaaacgt tatattttaa attttttttc agtcttcttt aacttttttt
    36121 tgcaaaatta aaaaaatttg gttaaattca atgggttccc ttacattttt agttttgacc
    36181 taaatgatag ttgtccaaat ctttcatttc aagttatagt taaagaaaat gaaaaagaag
    36241 gtgtgtaatg gaaaacgaaa ctgacgttat ttatattcat cctcaaaaac gaatcgtttc
    36301 acagaaaaga aagtattttt atcttggttt tacaggtgtt ttttttcttt ttattggctt
    36361 gctttctaat actcctactg ataattggag tggattgcta actattctta cctcgccaag
    36421 caatcttttg acagattatt ttgctttagg cggtttcgga agcgcattca taaatgtagg
    36481 gattttgacc ttactttctg tgcttttagc ttatcggcac aaggtgattt taaacggtcc
    36541 cttatttgct tcgattctaa cagttaccgg tttctctttt tttggcaaaa acctttataa
    36601 ttctatctca atcattttag gtgtgtatct atacgctgta tttgtcaata aacctttttc
    36661 tcagtacatc atgatcggac tgtttggtag cgcattatca ccggtcgtta gttatatcac
    36721 ttttggtatg cgttttcctt tactgatagg tattttattg gggaatttag ctggcatagc
    36781 aattggtcta cttcttccgc ccttagctgc tcaaacttta gttttccatc gtggattcac
    36841 actttataat attggattca cctctgggtt gattgcgatg acttttactg ctgttttgag
    36901 gttattcagc tattcaatcg tcgaaaatac attggttttc aatgaatatc actttcctct
    36961 catttggatt atttttggct ttttttcgct gacagtcgga attggtttct attataattc
    37021 ctttcggctt ttggggattc gagaaatttt cgattcatct ggaaaattga caactgattt
    37081 cattgctaat tctggtattg gtgctacttt gatcaacata ggcttagttg gtctgatgct
    37141 gtcttcttat gttttgctgg ttggcggtca gttaaatgga cctgtcatcg gtgctatttt
    37201 atctgcggtg ggtttcagtg cttttggttg tcatttaaaa aacagttttc cgatactagt
    37261 cggaatattt attgcttctt tgtttggaac ttttcatgag atcaccagta ctggcatgct
    37321 tgttgcagct gtgtttggga caggtctcgc gcccataagc ggtttttacg gcagttttta
    37381 cggtgtaata gctggtgtac tacatattgc tcttgtccat aacgtcagca ctttgcacgg
    37441 aggactaaat ctatataaca gtggtttttc aactggtttc gttgcaggta tccttgtacc
    37501 gatactagat aactttaccg ctgtcagaaa ggaaaaaaag acacttggaa aaagaattat
    37561 caaaaagaat catcgatgag cttcttactt atttatattc ccatgaaata actgaaatac
    37621 gtattggagt agaatttgct gaggaagggc ttttcataga gatacagggt acggcagaaa
    37681 ctcagccaga agatttagat gtacttgctg atttgctgaa caatcctcgg gaccctagta
    37741 tggaaatcta ttatcaagaa ctattaggta atccccgaca tgatcgggaa gattatcatc
    37801 tgttaggtag tttgattgat gacgtggaaa tcatgtatgc ccagccgatt atcaatatta
    37861 agatctttcg acgtgcttct cattcctatt attaacccta ttttctattt ataaaagact
    37921 gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta ctttcaggag ctgttcaagc
    37981 ttgacattat tgattttcca atccttcata cacttcatct gttacaggtt ctaaaaactc
    38041 tgaattacca gcagtgatcg ccaaatgttc aaaccaactg tcttttgtcg caccatgcca
    38101 atgtttaatc cctttgtgcg ttacgacgac atctccagcc attaatcgtc ttgctggctt
    38161 tccttcttcc tgataccagc cttctcctcc ggtcacaagt aagatttgat agccgtcttt
    38221 atggacatgc cagtgattgc gacaacctgg ttcaaaagtt acattaccta caccaacaga
    38281 aacttcggga tcattcacta acatattcag atagctttac ccggtaaata cttgagcgta
    38341 agcttcgttt ttttcacctg ctggaaaaat cacgccattg tgtatttctt cgtgttttgt
    38401 cgtcatttac gcctctttct tatcttcgct attttcttct aaatagattt cttttgctaa
    38461 actaaaagct gaccaagctt ttggccatcc tacataaaaa gctaaatggg taatgatttc
    38521 tgctatttct tcactagtaa tgccattttg cttgccaatc ttcagatgag ctggcaattg
    38581 ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg atactacgct gatgagcaga
    38641 taattcttct ctggaccata cttgtccaaa taaaacatca tcattcaatt ctgcaaattt
    38701 tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt ttttctgcca tttctcttat
    38761 tctcccttca cgactgttag gtgattcaac tatggttcta aggcaacttt attttctatt
    38821 gtttcttcgc atttcatttt aaatgtagtc tttcagcctg tccaatgctt agtttattca
    38881 gtcgatatgc tttttaagca tagttgctaa cgcttttttt a
//
LOCUS       DASJPH010000013        37331 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJPH010000013 DASJPH010000000
VERSION     DASJPH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.013"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..1012
                     /locus_tag="U3995_000642"
     CDS             149..1012
                     /locus_tag="U3995_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1795988.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1141..2763)
                     /locus_tag="U3995_000643"
     CDS             complement(1141..2763)
                     /locus_tag="U3995_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1795989.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3012..3533
                     /locus_tag="U3995_000644"
     CDS             3012..3533
                     /locus_tag="U3995_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1795990.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="U3995_000645"
     CDS             complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="U3995_000645"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1795991.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5614..6432
                     /locus_tag="U3995_000646"
     CDS             5614..6432
                     /locus_tag="U3995_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1795992.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6535..6732)
                     /locus_tag="U3995_000647"
     CDS             complement(6535..6732)
                     /locus_tag="U3995_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795993.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6901..7479
                     /locus_tag="U3995_000648"
     CDS             6901..7479
                     /locus_tag="U3995_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1795994.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7574..7711
                     /locus_tag="U3995_000649"
     CDS             7574..7711
                     /locus_tag="U3995_000649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1795995.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8524..9027)
                     /locus_tag="U3995_000650"
     CDS             complement(8524..9027)
                     /locus_tag="U3995_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1795996.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9386..10411
                     /locus_tag="U3995_000651"
     CDS             9386..10411
                     /locus_tag="U3995_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1795997.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10734..11612
                     /locus_tag="U3995_000652"
     CDS             10734..11612
                     /locus_tag="U3995_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1795998.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12088..12204
                     /locus_tag="U3995_000653"
     CDS             12088..12204
                     /locus_tag="U3995_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1795999.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13132..13470
                     /locus_tag="U3995_000654"
     CDS             13132..13470
                     /locus_tag="U3995_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796000.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13564..13794
                     /locus_tag="U3995_000655"
     CDS             13564..13794
                     /locus_tag="U3995_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796001.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14194..14565
                     /locus_tag="U3995_000656"
     CDS             14194..14565
                     /locus_tag="U3995_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1796002.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14839..15531)
                     /locus_tag="U3995_000657"
     CDS             complement(14839..15531)
                     /locus_tag="U3995_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1796003.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15534..16319)
                     /locus_tag="U3995_000658"
     CDS             complement(15534..16319)
                     /locus_tag="U3995_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1796004.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16556..17956
                     /gene="lacG"
                     /locus_tag="U3995_000659"
     CDS             16556..17956
                     /gene="lacG"
                     /locus_tag="U3995_000659"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1796005.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17981..18298
                     /locus_tag="U3995_000660"
     CDS             17981..18298
                     /locus_tag="U3995_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1796006.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18311..19984
                     /locus_tag="U3995_000661"
     CDS             18311..19984
                     /locus_tag="U3995_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1796007.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19998..21629
                     /locus_tag="U3995_000662"
     CDS             19998..21629
                     /locus_tag="U3995_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1796008.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22120..23685)
                     /gene="guaA"
                     /locus_tag="U3995_000663"
     CDS             complement(22120..23685)
                     /gene="guaA"
                     /locus_tag="U3995_000663"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1796009.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23744..24667)
                     /locus_tag="U3995_000664"
     CDS             complement(23744..24667)
                     /locus_tag="U3995_000664"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1796010.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24863..25468
                     /locus_tag="U3995_000665"
     CDS             24863..25468
                     /locus_tag="U3995_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1796011.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25776..26792
                     /locus_tag="U3995_000666"
     CDS             25776..26792
                     /locus_tag="U3995_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1796012.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26933..28234
                     /locus_tag="U3995_000667"
     CDS             26933..28234
                     /locus_tag="U3995_000667"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1796013.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28280..28942
                     /gene="deoC"
                     /locus_tag="U3995_000668"
     CDS             28280..28942
                     /gene="deoC"
                     /locus_tag="U3995_000668"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1796014.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28963..29361
                     /locus_tag="U3995_000669"
     CDS             28963..29361
                     /locus_tag="U3995_000669"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1796015.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29427..30509
                     /locus_tag="U3995_000670"
     CDS             29427..30509
                     /locus_tag="U3995_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1796016.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30721..32283
                     /locus_tag="U3995_000671"
     CDS             30721..32283
                     /locus_tag="U3995_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796017.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32276..33409
                     /locus_tag="U3995_000672"
     CDS             32276..33409
                     /locus_tag="U3995_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796018.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33409..34365
                     /locus_tag="U3995_000673"
     CDS             33409..34365
                     /locus_tag="U3995_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796019.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34464..35630
                     /gene="deoB"
                     /locus_tag="U3995_000674"
     CDS             34464..35630
                     /gene="deoB"
                     /locus_tag="U3995_000674"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1796020.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35654..36472
                     /locus_tag="U3995_000675"
     CDS             35654..36472
                     /locus_tag="U3995_000675"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1796021.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36489..37196
                     /gene="deoD"
                     /locus_tag="U3995_000676"
     CDS             36489..37196
                     /gene="deoD"
                     /locus_tag="U3995_000676"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1796022.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11638 a         6519 c         7896 g        11278 t
ORIGIN      
        1 ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg
       61 aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt
      121 aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga
      181 tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt
      241 gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa
      301 tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga
      361 atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg
      421 attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga
      481 agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt
      541 attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct
      601 ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga
      661 acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc
      721 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
      781 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      841 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      901 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      961 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
     1021 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
     1081 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
     1141 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
     1201 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
     1261 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
     1321 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
     1381 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
     1441 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
     1501 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
     1561 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
     1621 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
     1681 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1741 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1801 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1861 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1921 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1981 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     2041 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     2101 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     2161 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     2221 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     2281 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     2341 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     2401 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     2461 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     2521 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     2581 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     2641 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     2701 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2761 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2821 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2881 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2941 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     3001 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     3061 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     3121 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     3181 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     3241 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     3301 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     3361 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     3421 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     3481 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     3541 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     3601 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     3661 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3721 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3781 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3841 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3901 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3961 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     4021 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     4081 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     4141 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     4201 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     4261 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     4321 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     4381 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     4441 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     4501 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     4561 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     4621 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     4681 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4741 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4801 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4861 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4921 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4981 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     5041 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     5101 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     5161 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     5221 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     5281 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     5341 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     5401 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     5461 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     5521 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     5581 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     5641 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     5701 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5761 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5821 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5881 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5941 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     6001 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     6061 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     6121 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     6181 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     6241 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     6301 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     6361 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     6421 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     6481 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     6541 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     6601 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     6661 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6721 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6781 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6841 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6901 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6961 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     7021 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     7081 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     7141 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     7201 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     7261 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     7321 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     7381 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     7441 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     7501 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     7561 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     7621 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     7681 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7741 atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg
     7801 aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat
     7861 aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga
     7921 taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt
     7981 gtggtttttg gaataaaaaa ataacatatc tcattttaca cttttgttat tttatactta
     8041 aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact
     8101 gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc
     8161 aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata
     8221 aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc
     8281 ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa
     8341 aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc
     8401 ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa
     8461 taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg
     8521 attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa
     8581 taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt
     8641 tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt
     8701 gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag
     8761 gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac
     8821 cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc
     8881 aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg
     8941 tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc
     9001 taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg
     9061 aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg
     9121 aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag
     9181 ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt
     9241 tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt
     9301 tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt
     9361 ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca
     9421 agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg
     9481 ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt
     9541 ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg
     9601 actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt
     9661 tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa
     9721 accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat
     9781 cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc
     9841 ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt
     9901 aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc
     9961 cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac
    10021 cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt
    10081 ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc
    10141 gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct
    10201 agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc
    10261 tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt
    10321 cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta
    10381 tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt
    10441 atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat
    10501 taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct
    10561 atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt
    10621 ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta
    10681 caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt
    10741 tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg
    10801 cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca
    10861 tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg
    10921 cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt
    10981 atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa
    11041 ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa
    11101 caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct
    11161 ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa
    11221 gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga
    11281 ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt
    11341 accattacca agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt
    11401 tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg
    11461 aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt
    11521 atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca
    11581 ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga
    11641 gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    11701 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
    11761 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt
    11821 ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta
    11881 acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag
    11941 ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt
    12001 ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac
    12061 aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc
    12121 cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg
    12181 aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa
    12241 cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt
    12301 gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat
    12361 tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt
    12421 attatctgta gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat
    12481 cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg
    12541 taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc
    12601 aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc
    12661 taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct
    12721 atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa
    12781 agcagttact attgctagaa taacatcaag gaataataag ttttggtttt cagataatag
    12841 tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg
    12901 tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta
    12961 gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat
    13021 aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact
    13081 ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa
    13141 ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat
    13201 caggaaaata ttgttccgga gactccatta gctagtgagc caattactga tattgatgga
    13261 aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta
    13321 aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat
    13381 atttatactg atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct
    13441 actgataaaa agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa
    13501 ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc
    13561 gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga
    13621 gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca
    13681 tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga
    13741 atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt
    13801 tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt
    13861 aatattacta actttaatgc agtataaaga gtatccagag tattttattt taacttttgt
    13921 atatttagta agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa
    13981 agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa
    14041 aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact
    14101 gataatgaaa tttctcctgg ttgggaaaac gaagttggtt accctgtaga agaatttttt
    14161 caagattact ttgcggagta tgccgatagg ataatgagag attacgtaac agataaaact
    14221 caagaagtca tcaatcaatc ccagtcatta aatgaattat ctgataaggc gaaagaagaa
    14281 gcagttgcca ttatttcttc agggattagt gactatattg atatgagtgg tgaaatcaat
    14341 tatggcgata aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg
    14401 aaggcagatc ttaagtttaa tgatacttac ctagtaggaa aattaattaa tatccttggg
    14461 acagagaaag atacggaaac gtttatttcc tctttgtcta tgttgtttac agataagcat
    14521 ttttcaactc agcttggtgg agaggaacta gtggaaatct tttaaccatt acgcccagca
    14581 ttaataaaag tatatcaaaa aagtactcct gatgattttt aagattggtt tgagaaataa
    14641 tgtgaaaaca ctatttttaa tgttaattat agatagagat gaacgcgtca tatggttaaa
    14701 agaatgaaaa agtcaaagat acactgctat atttcactaa ggatatagca gtgtatcttt
    14761 catgtcgttt cactggcagt tttttataaa aggaggtgtg catttcagtt gcttttcttt
    14821 ggtagttagt tgaatcgttt aagaattaac ggatgtggca gacaggtgct ctgtccaaaa
    14881 accagtaaaa tctttgatgg ttccatcttt atttagctgt gcctgttcaa atactgtttt
    14941 gtttttaccg aatggatcaa ttgcaataat tgttccgatc cccgcacgtt tggcagcaag
    15001 taatcctgag taagcatctt cgataacaag acaatcagcc ggagctaagc tcagtttgtc
    15061 tgccgcttta agaaaaatat ccggttgcgg tttcccaggg aaagagccgt cgtcatagac
    15121 aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa aagcaacatt
    15181 ctctttgaca gtagccgtgg caatcgttaa tggaatctgt tggtcagtca gctggtcaag
    15241 agtcgcttca agcccttttg tcaactgaag ctcgttttca ttttgcaagc aaagcatacg
    15301 ataatagctt tctttttctt ctgacaatac ggcgatctca gaggcggtca aatgctctga
    15361 aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga atatttttta aaatatcctc
    15421 gtctgataaa ccatcttttg tatgatcatg tatcatatgc agccaagcag cttcatgcag
    15481 atgagagtcc aaaaacattg ttccgttgaa atcaaatata atccctttca tagttacgct
    15541 cctatagtgg tattttcggt tgaatcagca atttgcagat cgacatattg tgcatagtgc
    15601 tcttgaacct cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta
    15661 taaaacacat aaaaatcatc tcgattgaat tttttattat caacgagtag atatttctta
    15721 cgggaattat ttagagcgat ctgctgtgct tctccttctt cggtactgta agtagagatc
    15781 gcttcgttgt gaacgccatt tgaactgata aatgcttttg taaactttag tcttcttaaa
    15841 ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc accgccaatc
    15901 agtagaattt ctggtccatc tgcttgagca agaatatcaa aaactggata attatttgta
    15961 atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt tgtacctggt
    16021 cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt
    16081 ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa gttcttgatc taaagtgtag
    16141 gaaagacttt gagcccctcc atggacacgc agcagcttgc ccgatttttc taattcatct
    16201 aaatctctgc gaacggtcat atcagaaaca tctaattgat tgatgatttc attaacggta
    16261 aggattcctt ttttattgac catttctacg atagtaagca atctttcttt tttcaacatt
    16321 gtcttcacct ctaatttaag gataatctat catataaacg gacaacagtc aatctaattc
    16381 gaacaaaagt aaacatttag ggtggttttt atgtttgtta caatagattt tattgctgtt
    16441 ttctggtttt tatgattatt tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac
    16501 aatgcaatca gttagaaaga gcgaaaactg attatagatt tgaggagaga tgtacatgct
    16561 taaattacca gaagatttta tttttggcgg agccacagca gcctatcagg ttgaaggagc
    16621 cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac tttttagagg aacaagggcg
    16681 attcagtccc gatccggcaa gtgattttta tcatcaatat gcaaaggaca ttgaattgtg
    16741 tgaacgtttt ggtgtaaatg gtctaagatt atccattgct tggagccgca tttttcctga
    16801 tggagctggg aaaccaaatc cagaaggaat cgctttttat catcgtgtgt tcgaagaatg
    16861 taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat tttgataccc ctaaacgttt
    16921 gtttgaccat ggagattttt tgaatcgaga aacaatagaa gcatttgttt cctatgccat
    16981 tttttgtttt catgaattca aagaagtcaa ggtgtggagt acctttaatg aaatctatcc
    17041 agttgcaacc aatcaatatt tgttaggtgt tttcccacca ggaatcaaat atgattttac
    17101 aaagatcgtc gcctgtcttc ataatatgat ggttgctcat gcccgtgtag tgaactattt
    17161 caaagaaaac gaacttccgg gtgaaattgg tgtagttcac tcattagaga cgaaatatgc
    17221 ggcaacagac gcgccggaag ataagcatgc tgccttttta gacgatgcat tgtcgatccg
    17281 tttcttatta gatgctacct atttaggcta ttattcaact gaaaccttga ctgctcttga
    17341 tgagatatgt gaagcaaatc aggccagcta tcattttcct gaagaagatt tcgttgaatt
    17401 gaaaaaagca tccacgcgta atgactactt agggatcaat cactaccaat gccattttgt
    17461 caaagcttac gatggagaaa atgcaatcca tcataacggt acgggtgaaa aagggacctc
    17521 tgtttataaa gtgaagggaa tcggagaacg tatttataaa gaaggaattc ctcgaactga
    17581 ttgggactgg ctgatttatc cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga
    17641 ttacccacat tacaacaaaa tctatatcac tgagaacggg atggggtata aagatcagtt
    17701 tgaagatggc attattatgg atcagccgag aatcgactat ttgagagtct atttggaatc
    17761 cttaagcaag gcgataacag ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat
    17821 ggatcttttc tcatggacaa atgggtataa caaaaggtat ggcttgtttt atgttgattt
    17881 tgaaacccag aagcgttacc caaaagaatc agcttactgg tacaagttgg tcagtgaaac
    17941 caaaacgatt atttaataag tgataatgga ggaagaaaag atgaataaag aggaattaca
    18001 aatgcttggt tttgagatcg ttgcatattc aggcgacgct cgcagtacat tattgaaatt
    18061 attgaaagaa gtaagacaag gaaactttga acatgttgat tcggcgttga aagaagccga
    18121 cgaaaattta acccttgcac ataattcgca aacaaaaatt ttagcggaag aagcatctgg
    18181 gaaagagatg gagatgggat tcatttttat ccatgggcag gatcatttaa tgaccacttt
    18241 attgctgaga gatctgattc aagactttat tgtactgtac agacagaatc aaggatagga
    18301 gaagaacgct atggatggaa tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa
    18361 aatctcacgt aatagttatt taggagccgt tcgtgacgga tttttaacag cgatgccagc
    18421 tattttattt tcaagcattt tcattttgat tgctgcggtt cctgagattt ttggttacca
    18481 atggcctgaa tctgtctcca catggctatg gaaagtctat aattattcga tgggtgttgt
    18541 agggttactc gtttcagcaa ccactgctcg ctgcttggct gattcgatga atcgtcgctt
    18601 accgaaaaac aaagtaatca acaccacctc tgtgatgctg gcctctgtgg taagtttcct
    18661 gttgttaagc gtctctccga ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg
    18721 actactggct tcctttgtcg ctgcattttt aacagttaat ttatataagt tttgtgtgct
    18781 gaaagatatt accattaaaa tgcctaaaga agttcctgga acgatttcgc aaaccttccg
    18841 agatattttt cctttctctt tttcagtatt tgcggccgtt ttaattgatt taggcattcg
    18901 acaaatcttc caaatctcat ttgcagaagc aatcattacg gtgctgcagc cgctatttac
    18961 agcagctgac gggtacttag gaatcgctat tatctgggga gcgatggcgc tgttttggtt
    19021 tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt gcggcaatta tctatgccaa
    19081 tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa gcttctaaag tactgacggt
    19141 gggtttaggg aattttgtag gaactatggg aggaactggg gcaacattgg ttgttccttt
    19201 tatcttccta ctctttgcta agtctaagca gttgaaggca gttgggaaag cttcctttat
    19261 tccagtaagt ttcgctgtca acgaaccatt gctatttgcg gcacccatga ttttgaaccc
    19321 ttattttttt attccatttt tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt
    19381 tgtcgatgta ttgggcatga acagctttat gtacgttttg ccttgggcaa ctccagcacc
    19441 gatcggtttg gttctaggaa cgggcatggg tatcttagct tttgttctag cagcggttct
    19501 aatcgtcgtt gacttcctga tctatctacc attttgcaaa gcatacgatg ctgtcttagt
    19561 agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt
    19621 acgtgaggaa gtacctgtgg cagcgagtca agttatcagc gatgcaaata aagaaatcaa
    19681 tgtgttggta ctttgtgctg gcgcagggac aagtgggatg ttagctaatg ctttaaaaaa
    19741 aggtgcatct gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg gttctcatta
    19801 cgaaatgctg caggattttg atatggtggt cttagcaccg caagtcaatt cttactatga
    19861 tgatatcaaa gaagacaccg atcgcttagg aatcaagctg gttgcgacaa aaggagcaga
    19921 atatatcgga ttaacgagag atcctgaaaa agctgttcag tttattctga accaatttca
    19981 ataaaggagg agccataatg aaaatgggaa tttggttgcg taaagctgtt ttatttgcag
    20041 ctgtgttagg ggctttgggg gctgtttcaa aaactgttga agcatctgcc agcgaattta
    20101 ttcgcggagc agatatttcg atcctgtcag atatggaaaa atctggtgca aaatattatg
    20161 aagacgatat ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc aactatgttc
    20221 gactgcgttt gtggcaggat ccttataata gtaatggaga aagttatgga gcagggacaa
    20281 atgatctgga aacgacgatt gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc
    20341 tgctggattt tcactacagt gatttttggg ttgatcctgg gaaacagaat cttcctaaag
    20401 catggcaagg tctgaccttt gaagaaatga atacggcatt gtacgactat acaaagaacg
    20461 ttctgtcgga aatgaagcag ctggacgttt atcctgatat ggttcagatc ggcaatgaac
    20521 tgaatagcgg aatgctctgg ccttatggca agtcttgggg agaaggcggg ggagaatttg
    20581 atcgattggc tgcttttttg aagtctggta tccaagctgt tcgagacact caacctaaaa
    20641 caactccagt aatgctccac ttagctgatg gtggcgatac aggagcattt acttggtggt
    20701 ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt
    20761 attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg
    20821 gtaaagaagt gatcgtagtt gaaacagctt atgggcatac aacagcgaat gccgatacta
    20881 tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg atatgaacca acaccgacag
    20941 gacaagcaga gtatttgctg gatcttgccg atcggattca ggcagtaccg aataatcgcg
    21001 gggcgggctt cttctattgg gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac
    21061 aagcaggtat gaattatctc agtgttcaat ccactatggg aaacgaatgg gacaaccaag
    21121 cgatgtttga ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa caagcagggg
    21181 cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg accagtcctt
    21241 ccaattggca aacctggttt caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg
    21301 gcactttttt tggggactac aaactaactt tttggcagga tcaagcatac gaggcatcag
    21361 cttatcaaaa tgttagtggc ataagcaaag gaacttacac attgagtgcc tatgtaatgt
    21421 ctgggggaga gtttgatact aatcagttat atatcaaaaa ttatggtgga gcggaactaa
    21481 atactgcggt tgttaaaaca agtgaatgga caaaaattac aatcaaagac attccaatta
    21541 caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct tggacgaacg
    21601 ttgattttgt ttcattggtg aaacaataga ctgcggatcg tttcgattca agatcggagg
    21661 ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc
    21721 caaaaataac cagaaacgat ttccagtcat ttttaccaaa acgagtaaag caccagactt
    21781 catctacact ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa
    21841 attgcaaaaa gttgtgcaga taacataaag tttgtttaag aaagggtaac cttagaattc
    21901 cgtcgtgaat atgatttgag cacttagatg gatagaaaaa agttctcgga aaacagttcg
    21961 tttttcgaga acttcttttt atgataagaa tgaaacaaat taacttatat tatagtctct
    22021 tttggtaggg aaccatctta tttttaatga aaatcaagca ttttagctat tgaagaatgt
    22081 tgatttatag ggattccccc tccatattat gttggctctt tattcccact ccactgttgc
    22141 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
    22201 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
    22261 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
    22321 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
    22381 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
    22441 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
    22501 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
    22561 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
    22621 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
    22681 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
    22741 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
    22801 tttcaacttg tctaagaagc gatctcttgc attgactttg atgatgttca agccgaattt
    22861 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
    22921 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
    22981 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
    23041 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
    23101 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
    23161 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
    23221 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
    23281 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
    23341 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
    23401 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
    23461 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
    23521 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
    23581 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
    23641 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
    23701 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
    23761 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
    23821 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
    23881 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
    23941 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
    24001 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
    24061 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
    24121 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
    24181 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
    24241 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
    24301 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
    24361 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
    24421 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
    24481 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
    24541 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
    24601 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
    24661 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
    24721 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
    24781 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
    24841 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
    24901 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
    24961 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
    25021 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
    25081 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
    25141 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
    25201 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
    25261 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
    25321 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
    25381 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
    25441 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
    25501 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
    25561 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
    25621 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
    25681 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
    25741 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
    25801 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
    25861 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
    25921 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
    25981 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
    26041 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
    26101 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
    26161 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
    26221 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
    26281 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
    26341 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
    26401 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
    26461 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
    26521 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
    26581 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
    26641 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
    26701 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
    26761 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
    26821 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
    26881 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
    26941 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
    27001 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
    27061 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
    27121 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
    27181 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
    27241 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
    27301 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
    27361 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
    27421 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
    27481 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
    27541 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
    27601 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
    27661 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
    27721 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
    27781 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
    27841 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
    27901 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
    27961 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
    28021 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
    28081 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
    28141 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
    28201 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
    28261 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
    28321 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
    28381 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
    28441 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
    28501 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
    28561 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
    28621 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
    28681 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
    28741 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
    28801 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
    28861 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
    28921 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
    28981 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
    29041 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
    29101 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
    29161 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
    29221 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
    29281 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
    29341 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
    29401 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
    29461 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
    29521 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
    29581 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
    29641 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
    29701 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
    29761 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
    29821 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
    29881 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
    29941 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
    30001 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
    30061 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
    30121 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
    30181 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
    30241 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
    30301 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
    30361 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
    30421 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
    30481 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
    30541 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
    30601 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
    30661 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
    30721 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
    30781 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
    30841 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
    30901 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
    30961 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
    31021 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
    31081 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
    31141 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
    31201 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
    31261 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
    31321 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
    31381 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
    31441 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
    31501 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
    31561 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
    31621 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
    31681 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
    31741 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
    31801 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
    31861 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
    31921 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
    31981 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
    32041 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
    32101 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
    32161 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
    32221 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
    32281 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
    32341 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
    32401 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
    32461 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
    32521 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
    32581 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
    32641 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
    32701 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
    32761 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
    32821 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
    32881 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
    32941 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
    33001 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
    33061 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
    33121 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
    33181 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
    33241 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
    33301 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
    33361 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
    33421 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
    33481 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
    33541 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
    33601 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
    33661 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
    33721 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
    33781 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
    33841 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
    33901 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
    33961 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
    34021 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
    34081 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
    34141 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
    34201 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
    34261 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
    34321 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
    34381 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
    34441 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
    34501 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
    34561 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
    34621 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
    34681 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
    34741 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
    34801 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
    34861 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
    34921 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
    34981 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
    35041 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
    35101 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
    35161 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
    35221 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
    35281 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
    35341 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
    35401 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
    35461 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
    35521 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
    35581 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    35641 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    35701 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    35761 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    35821 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    35881 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    35941 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    36001 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    36061 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    36121 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    36181 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    36241 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    36301 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    36361 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    36421 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    36481 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    36541 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    36601 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    36661 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    36721 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    36781 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    36841 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    36901 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    36961 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    37021 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    37081 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    37141 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    37201 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    37261 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    37321 gaggttgtga a
//
LOCUS       DASJPH010000014        37151 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJPH010000014 DASJPH010000000
VERSION     DASJPH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.014"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2301)
                     /locus_tag="U3995_000677"
     CDS             complement(148..2301)
                     /locus_tag="U3995_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1796023.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="U3995_000678"
     CDS             complement(2595..3443)
                     /locus_tag="U3995_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1796024.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="U3995_000679"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="U3995_000679"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1796025.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="U3995_000680"
     CDS             complement(5944..6123)
                     /locus_tag="U3995_000680"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1796026.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="U3995_000681"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="U3995_000681"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1796027.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="U3995_000682"
     CDS             8271..10550
                     /locus_tag="U3995_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1796028.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="U3995_000683"
     CDS             complement(10643..11290)
                     /locus_tag="U3995_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796029.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="U3995_000684"
     CDS             complement(11469..12188)
                     /locus_tag="U3995_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796030.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="U3995_000685"
     CDS             complement(12319..13470)
                     /locus_tag="U3995_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1796031.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="U3995_000686"
     CDS             complement(13687..14427)
                     /locus_tag="U3995_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1796032.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="U3995_000687"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="U3995_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1796033.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="U3995_000688"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="U3995_000688"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1796034.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="U3995_000689"
     CDS             complement(15388..16038)
                     /locus_tag="U3995_000689"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1796035.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="U3995_000690"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="U3995_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1796036.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="U3995_000691"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="U3995_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1796037.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="U3995_000692"
     CDS             complement(17481..18008)
                     /locus_tag="U3995_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1796038.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="U3995_000693"
     CDS             complement(18188..18973)
                     /locus_tag="U3995_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1796039.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="U3995_000694"
     CDS             complement(18966..19835)
                     /locus_tag="U3995_000694"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1796040.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="U3995_000695"
     CDS             complement(19836..21215)
                     /locus_tag="U3995_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1796041.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="U3995_000696"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="U3995_000696"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1796042.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="U3995_000697"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="U3995_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1796043.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="U3995_000698"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="U3995_000698"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1796044.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="U3995_000699"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="U3995_000699"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1796045.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="U3995_000700"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="U3995_000700"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1796046.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="U3995_000701"
     CDS             complement(25173..25397)
                     /locus_tag="U3995_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1796047.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="U3995_000702"
     CDS             complement(25450..26412)
                     /locus_tag="U3995_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1796048.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="U3995_000703"
     CDS             complement(26422..26862)
                     /locus_tag="U3995_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1796049.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="U3995_000704"
     CDS             27168..28133
                     /locus_tag="U3995_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1796050.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="U3995_000705"
     CDS             complement(28190..28420)
                     /locus_tag="U3995_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1796051.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="U3995_000706"
     CDS             complement(28506..29393)
                     /locus_tag="U3995_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1796052.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="U3995_000707"
     CDS             complement(29482..30705)
                     /locus_tag="U3995_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1796053.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31768)
                     /locus_tag="U3995_000708"
     CDS             complement(30722..31768)
                     /locus_tag="U3995_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1796054.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="U3995_000709"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="U3995_000709"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1796055.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="U3995_000710"
     CDS             complement(33659..33925)
                     /locus_tag="U3995_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1796056.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="U3995_000711"
     CDS             34404..36638
                     /locus_tag="U3995_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1796057.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="U3995_000712"
     CDS             36786..37106
                     /locus_tag="U3995_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1796058.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10450 a         8038 c         6479 g        12184 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcgtatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatagtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tcaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa a
//
LOCUS       DASJPH010000015        37146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJPH010000015 DASJPH010000000
VERSION     DASJPH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.015"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..384)
                     /locus_tag="U3995_000713"
     CDS             complement(<1..384)
                     /locus_tag="U3995_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796059.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="U3995_000714"
     CDS             complement(569..967)
                     /locus_tag="U3995_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1796060.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="U3995_000715"
     CDS             complement(964..1737)
                     /locus_tag="U3995_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1796061.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="U3995_000716"
     CDS             complement(1734..2417)
                     /locus_tag="U3995_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1796062.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="U3995_000717"
     CDS             complement(2584..3471)
                     /locus_tag="U3995_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796063.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="U3995_000718"
     CDS             complement(3468..4337)
                     /locus_tag="U3995_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796064.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="U3995_000719"
     CDS             complement(4474..5760)
                     /locus_tag="U3995_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1796065.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="U3995_000720"
     CDS             6033..6611
                     /locus_tag="U3995_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1796066.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="U3995_000721"
     CDS             6611..7540
                     /locus_tag="U3995_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1796067.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="U3995_000722"
     CDS             7537..9273
                     /locus_tag="U3995_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1796068.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U3995_000723"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U3995_000723"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1796069.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="U3995_000724"
     CDS             complement(10169..10678)
                     /locus_tag="U3995_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1796070.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U3995_000725"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U3995_000725"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1796071.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="U3995_000726"
     CDS             complement(11979..13070)
                     /locus_tag="U3995_000726"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1796072.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U3995_000727"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U3995_000727"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1796073.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U3995_000728"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U3995_000728"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1796074.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U3995_000729"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U3995_000729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1796075.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="U3995_000730"
     CDS             complement(17033..18007)
                     /locus_tag="U3995_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1796076.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="U3995_000731"
     CDS             complement(17988..19871)
                     /locus_tag="U3995_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1796077.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="U3995_000732"
     CDS             complement(19889..22114)
                     /locus_tag="U3995_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1796078.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="U3995_000733"
     CDS             22229..23263
                     /locus_tag="U3995_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796079.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="U3995_000734"
     CDS             complement(23330..24343)
                     /locus_tag="U3995_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1796080.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="U3995_000735"
     CDS             complement(24421..24609)
                     /locus_tag="U3995_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1796081.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="U3995_000736"
     CDS             complement(25049..25177)
                     /locus_tag="U3995_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1796082.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U3995_000737"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U3995_000737"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1796083.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="U3995_000738"
     CDS             complement(26664..27719)
                     /locus_tag="U3995_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1796084.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U3995_000739"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U3995_000739"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1796085.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U3995_000740"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U3995_000740"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1796086.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U3995_000741"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U3995_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1796087.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U3995_000742"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U3995_000742"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1796088.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U3995_000743"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U3995_000743"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1796089.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="U3995_000744"
     CDS             35481..37040
                     /locus_tag="U3995_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1796090.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10262 a         8066 c         6697 g        12121 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc cagcctcttc
    37141 tttttt
//
LOCUS       DASJPH010000016        36820 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJPH010000016 DASJPH010000000
VERSION     DASJPH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.016"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..381
                     /locus_tag="U3995_000745"
     CDS             46..381
                     /locus_tag="U3995_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796091.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(638..2221)
                     /locus_tag="U3995_000746"
     CDS             complement(638..2221)
                     /locus_tag="U3995_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796092.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2343..2927
                     /locus_tag="U3995_000747"
     CDS             2343..2927
                     /locus_tag="U3995_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796093.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2938..3183
                     /locus_tag="U3995_000748"
     CDS             2938..3183
                     /locus_tag="U3995_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796094.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3189..3911
                     /locus_tag="U3995_000749"
     CDS             3189..3911
                     /locus_tag="U3995_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796095.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4042..4785
                     /locus_tag="U3995_000750"
     CDS             4042..4785
                     /locus_tag="U3995_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796096.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4791..5156
                     /locus_tag="U3995_000751"
     CDS             4791..5156
                     /locus_tag="U3995_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796097.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5172..5702
                     /locus_tag="U3995_000752"
     CDS             5172..5702
                     /locus_tag="U3995_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796098.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5864..6172
                     /locus_tag="U3995_000753"
     CDS             5864..6172
                     /locus_tag="U3995_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796099.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            6168..6587
                     /locus_tag="U3995_000754"
                     /pseudo
     CDS             6168..6587
                     /locus_tag="U3995_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6724..7524
                     /locus_tag="U3995_000755"
     CDS             6724..7524
                     /locus_tag="U3995_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796100.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7561..7743
                     /locus_tag="U3995_000756"
     CDS             7561..7743
                     /locus_tag="U3995_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796101.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7854..8171
                     /locus_tag="U3995_000757"
     CDS             7854..8171
                     /locus_tag="U3995_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796102.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            8174..8406
                     /locus_tag="U3995_000758"
                     /pseudo
     CDS             8174..8406
                     /locus_tag="U3995_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8521..9000)
                     /locus_tag="U3995_000759"
     CDS             complement(8521..9000)
                     /locus_tag="U3995_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796103.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            9120..10457
                     /locus_tag="U3995_000760"
     CDS             9120..10457
                     /locus_tag="U3995_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1796104.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            10480..11007
                     /locus_tag="U3995_000761"
     CDS             10480..11007
                     /locus_tag="U3995_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796105.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10994..11557
                     /locus_tag="U3995_000762"
     CDS             10994..11557
                     /locus_tag="U3995_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796106.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11979..12716
                     /locus_tag="U3995_000763"
     CDS             11979..12716
                     /locus_tag="U3995_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796107.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12733..13149)
                     /locus_tag="U3995_000764"
     CDS             complement(12733..13149)
                     /locus_tag="U3995_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796108.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13542..14789
                     /locus_tag="U3995_000765"
     CDS             13542..14789
                     /locus_tag="U3995_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1796109.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15057..17447
                     /locus_tag="U3995_000766"
     CDS             15057..17447
                     /locus_tag="U3995_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HEN1796110.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            17496..19331
                     /locus_tag="U3995_000767"
     CDS             17496..19331
                     /locus_tag="U3995_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1796111.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            19400..20263
                     /locus_tag="U3995_000768"
     CDS             19400..20263
                     /locus_tag="U3995_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1796112.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            20253..21362
                     /locus_tag="U3995_000769"
     CDS             20253..21362
                     /locus_tag="U3995_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1796113.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            21701..22501
                     /locus_tag="U3995_000770"
     CDS             21701..22501
                     /locus_tag="U3995_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796114.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            22589..23395
                     /locus_tag="U3995_000771"
     CDS             22589..23395
                     /locus_tag="U3995_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796115.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            23627..24496
                     /locus_tag="U3995_000772"
     CDS             23627..24496
                     /locus_tag="U3995_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1796116.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            24516..24863
                     /locus_tag="U3995_000773"
     CDS             24516..24863
                     /locus_tag="U3995_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796117.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            24876..26138
                     /locus_tag="U3995_000774"
     CDS             24876..26138
                     /locus_tag="U3995_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796118.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            26218..27594
                     /locus_tag="U3995_000775"
     CDS             26218..27594
                     /locus_tag="U3995_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN1796119.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            27591..28220
                     /locus_tag="U3995_000776"
     CDS             27591..28220
                     /locus_tag="U3995_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796120.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            28232..28702
                     /locus_tag="U3995_000777"
     CDS             28232..28702
                     /locus_tag="U3995_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796121.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            28699..29181
                     /locus_tag="U3995_000778"
     CDS             28699..29181
                     /locus_tag="U3995_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796122.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            29178..29840
                     /locus_tag="U3995_000779"
     CDS             29178..29840
                     /locus_tag="U3995_000779"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1796123.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            29827..30195
                     /locus_tag="U3995_000780"
     CDS             29827..30195
                     /locus_tag="U3995_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN1796124.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            30276..30728
                     /locus_tag="U3995_000781"
     CDS             30276..30728
                     /locus_tag="U3995_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796125.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            31380..31433
                     /locus_tag="U3995_000782"
                     /pseudo
     CDS             31380..31433
                     /locus_tag="U3995_000782"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31437..32036
                     /locus_tag="U3995_000783"
     CDS             31437..32036
                     /locus_tag="U3995_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1796126.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            32052..32528
                     /locus_tag="U3995_000784"
     CDS             32052..32528
                     /locus_tag="U3995_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796127.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            32545..33447
                     /locus_tag="U3995_000785"
     CDS             32545..33447
                     /locus_tag="U3995_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796128.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            33520..33711
                     /locus_tag="U3995_000786"
     CDS             33520..33711
                     /locus_tag="U3995_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796129.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            33875..34462
                     /locus_tag="U3995_000787"
     CDS             33875..34462
                     /locus_tag="U3995_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796130.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            34462..35361
                     /locus_tag="U3995_000788"
     CDS             34462..35361
                     /locus_tag="U3995_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEN1796131.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            35361..36275
                     /locus_tag="U3995_000789"
     CDS             35361..36275
                     /locus_tag="U3995_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796132.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            36327..36542
                     /locus_tag="U3995_000790"
     CDS             36327..36542
                     /locus_tag="U3995_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796133.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        13825 a         4978 c         7288 g        10729 t
ORIGIN      
        1 acaatttcct aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta
       61 aatgaatgga aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat
      121 tgcttagaga aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat
      181 ccagaccaat ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc
      241 aatgatggaa aatattttat tattcaggag tatggaggac agttaactcc tttctatact
      301 acatgggaaa tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca
      361 atttcacaca ccgaatgtta attgattata actataaaaa aggattgaac attatgttca
      421 atcctttttt caatgaaatg ttttccgcca tttcattgtt tggtgcgatt tctattttat
      481 tatatcaaaa taaagagaga ttctaaagaa agaagaggga aatatataaa tcaagcgaca
      541 gtatatttat tattaaatgc tattgtgaga atataactaa ttagtttaat ttgaaagtaa
      601 atatacgcta taaaagtgca tcttgtgtta taaaaaatta ttttattttg tgaacataat
      661 ttaacaattc tttctcggaa ttgatttctt ttcttaaata gtcttcagac ttattaataa
      721 tattatctat tatgtctaga tacatatcac ttttcgattc agctaattca tacagttttt
      781 ttaattcttt atcctttaat tgaaaaccct gatccatagt cactgcattt ggatatagaa
      841 gcccactata atgtttacgt aaattcactt ggaatttctg attataaaag tctaacagat
      901 tagaaataaa aggcggaaaa ttactgtttt tgtaaaattg attatttcca tttaggttgt
      961 atagtaattt gttaaattgc tcctgattta atttattgta aatccatggt aatacttcca
     1021 tatccttatc tgctaacctg aatgaactag catcggtaag atgttcgatc ataaaatcta
     1081 tgaacatctc tttttcacca aaatataaat attgattata tattgtttct aatgttgaag
     1141 atggtagcga agaaactctc attttttgct ggagtatagg atcaactaga ctatttaagt
     1201 gattttgtaa ttcttcattg ttgtttccta gaactgcaag ggaacgtaaa cggtagtaat
     1261 attctatttc tgttagtact tgttgtttcg gtttaaaatc atttattgat atttgtaaat
     1321 tttgaattgt ctcgtctgaa aaatcatgtt gtaattctgg atgggtagct ataaaagtaa
     1381 gtaaaaagaa cccttttgta tacgtgatct gttgattttc accaagtgat ttattaattt
     1441 cctctcttga ttcagctgtg aacctatact ttgcaatttc tttttttaga atatttaaat
     1501 agaattcttt attatcaaaa attagggcat taagtagtct gaaagtactt tccctatctc
     1561 tatctgcttc ctcatttcca taagataatt gaaaaacaaa cttaaaggct gtcttacata
     1621 ggttttcttt ggctttttcg cttagtttat ttagatagct attttttagt gcagagtgaa
     1681 attcttcagg tgtaacagac atagcatgta tgcgacttgc atattgtata atttcgatcg
     1741 ttactttttt tcctaatatt ggatttttta ggaatataat ttcataggac ttccgtataa
     1801 catcctgaca agtttctttt gttatatctc ttaattccca agtattaccg ttgtaagtga
     1861 ctacaggatg agctgaataa tttctttgat ccttaatata ttttaaatcc tcatattcat
     1921 agctatcaat tagctcagtt gaactataag ctttttctag tagttccatt tcccattttg
     1981 gagagtttgg atcacttttt cttagtctag ctatatcttc taaaattttc ttagaagtat
     2041 tatcctcgta aatatttgct aaatcagata atttatcaat taaatctaaa ataagcgcac
     2101 tgtaattgac agctattgca gaacgataat ttccctgata aaaactactt ctaacttctt
     2161 ctaataatgg aataattcta ggatttttaa ctttttttat ttcatcatct aatataaaca
     2221 tataaatctc ctttttttat ttgtatgaca agtttaccat aaaaaaaaat taaaaagcga
     2281 gacatattta tgttgttgtg ttatcataat aacataaata tgtagagtga gatgatgaaa
     2341 aaatggattt gaaacaatcg ttttacatct ggttatatcc tgataatcct atgtcaataa
     2401 tggctgatat ttttgtagtt agcatcttat tgtatttatt ttatctaatt ttctttcaca
     2461 atttaatcgg gtacatgatt cttgattgga aaggaatgtt cgatatagaa agaaaaccat
     2521 ttgctaacaa tcaaacctat accgtcttcg aaagtggaga ttgggaagaa aatgaagttg
     2581 atgtgatcca tgaaattgga ttagctttat caggaataca agatgctgat gttttggtat
     2641 caatgacaac ggatactgga tacaacgctt atgaggtatt ttatcttatg tattttatat
     2701 ggcgtgatgg tcaagaaatt atcaatggtc gtgctgaaaa aacaatcaat tctaatactg
     2761 aaatttcttt ttgtatgggt gaaggggaaa tgattgtata taaagatggg tcagcaaaca
     2821 ttaaaacagc ctttggtgat attgaaacaa gtattaagga acttagggaa tatgtgcaga
     2881 acccatataa ttttcaatat ttagcaggaa gaataaattt agtttaggag taaaaatatg
     2941 aaaaaaatta taaatcaacc aacaaatcac ttgataattg aaggtgttat tttcgtaaca
     3001 atgtatttta ctgtaggaaa tggcatctca aatacaatca ttcaaaaaaa tccacaatac
     3061 ctattattta gtctttatgc ttggattgta caaatggttt gggtttccat attgatgctg
     3121 ttgttttatt ggatagtacg ggttattctt ttattgttaa acaaaagaaa aaaggatagg
     3181 tgaataaaat gaaatcaact aaattgaagg ttgtattgtt atcgacagca gcattaataa
     3241 cccttacagc atgtggtaca actaatagtg ctaataataa caaagacaca aagtctagta
     3301 gtacgatggt tactaaaaac agcactacat cggcatcaac aacaatgtct tcaatgactg
     3361 aaaataatgc tgaaaagtat caaaaatacg aagacattaa taaaggtcaa aagccggatg
     3421 aagtttataa caactttatt aatacagtaa aaaatgatga aaattatggg aatgatccat
     3481 tgcaaaataa aaatctgact atcgttcttt acaaacctga ttgtccagat tgtcaaaaag
     3541 cagaatcaaa aatcatgcaa gaaataaaag aaggatttag ttatgctcca acattaatgc
     3601 atgatattac cataaataaa ccagcttacg agtattacgc agttgctgta gatattactg
     3661 atggtgtacc tgagtgggta caaaacattc ctggtctggc tgatgagaac aatcaatatc
     3721 atacgccaac agttgcagta gtaacaccaa agaaagttaa cggcacacta tactgggatg
     3781 taatgggaat gacaacaaat acaactgatg aaatgattga aagtacaact aaatattata
     3841 tggatttggg agcatataaa ttatatcaac aatcagagcc tgttatggac gatcaatcca
     3901 aagttaaata gatctttagg gcgtaagccc tattttttta aaattaagtg ataacaaata
     3961 aatgatttct gaaacaaacg ttcttactca actactcgaa acacacaagc taattaacta
     4021 gattataaag gagaatgcgt aatgaaagaa atcagtgcaa atgaagctca tattccgcaa
     4081 aagaatcagg ttggtatata taaggtgctt catggtgaag gaatttataa aatacagtta
     4141 cttaccggtt acaataatgg tgcaaatggg aagtggttaa aggcaacaag tattcgtgaa
     4201 cttagaagtg aagtagaaaa aattaggctt ggtctcacaa gtgaagaaat agctaaactg
     4261 aagggaaata attcttttgt aattgaaaaa gaaaaggaaa aacatctact ttatcgtgag
     4321 ctgaaaattg aagaatacta tgaaattgat ttacctgaaa aattaagaat tattgatcaa
     4381 cgttcagaag aaaaatggaa aactgaaata tatccaagct taaatgacta caagacatca
     4441 ggaaagccca tgtataacta cacaggcttt ttaacctaca cacaatacaa atatgccttt
     4501 cgcaaattaa aaggcattaa acaagctgaa attgccctgt atgagatgaa aaaagtggat
     4561 agattgaaat tgattcaaga tttagtgcaa aatacatgga atcaagcgat tgtcgagcta
     4621 gaaaaagaaa atgggaaaat tttaactaat aaatatctga tcgtaaatga aattgaagaa
     4681 ttaattggtt tattttcatg tgaaaaaggt gaagtcttct tgaagtcatc accaacctat
     4741 tcaattttgg acagtccgta tcagtttata caggcaagga gatgagttag atgtctagct
     4801 tgaaagatga ctatatccaa gcaaaacaag agtatgaaaa aatacagatg ttgaaatctg
     4861 agaaagtcga attattttta acacaaaaat tagcagagga tcatattgct aacttagatg
     4921 ctaaaacttt agaggaatat gatcggcagt ttaatcagat acatcacaat atcattttgc
     4981 ttgttgagca ggcaaagcga aggtttgaaa aagcggaata tgatctatta gatcaaggaa
     5041 ttgtatgctt ggataaggga ttgcgaagat attatggtgc agagttaaaa aagcaaaatg
     5101 tagatagaaa tgcactaaaa catgaaatct tagaattgat aatgcaacga atttgaaggg
     5161 atagaaagca aatgtcacaa aacattagtg ctgtaaaaca attaatagaa gaagatttag
     5221 agaccacagt tcagagatta gcggaacgaa tccattttgc aaattatcaa accatctcta
     5281 actggtcaag caggaacaga aatccggaag aaattccaat gaaatttttt tatcgaatcg
     5341 gaaaagaata tggtgttgat ccgataaaaa ttttcgagaa gaccatgatt tattactatc
     5401 atggaacacc taacgaagtg ttagaacatc ttttggaaga ggcaaaagaa aaatcatcaa
     5461 tattagagct atcgcaaaat gacaaagtta ttcaagtgaa atgcgttgat ccaacaggaa
     5521 agattattgt cggttttgat attgaaagaa aatccaaaat agattttatt acacaattaa
     5581 aaaagataaa taatttagat attctaacgc ttagaccaga tcatgaacag ttgtttatga
     5641 gttttaaaaa acaattgaaa gaaatacaaa aaagatataa caaggtgttt cctaatgcgt
     5701 gaaaagctca aagaaatcag agttttttgg ataattaaca aagaatgtat catcttatat
     5761 actcttggat ttcttagcat gatcttgttt tggtggttat atttaaaaca gtaattggag
     5821 agggtcaaga ggatttttat caagataagg atgtggaaac tgcatgtgga ataatagatt
     5881 aaaaacagga ttgttattaa tagttatttc ctgcgctatg atgattggta tgaggataca
     5941 aagggaacaa tcctattttg aagtttctgc taacaacgta atagaaaaat gctattacgg
     6001 tcaacattat tggagtgagg aagtcagaga aaatatagat cgtgaatatg ttcaaagaat
     6061 tgtttgggat gcatattcta tcaaagatta tcctaaaagt ttaacgagta gattatttta
     6121 cagtgaaaaa gataatcaaa aactctcaga tttgatgatg aaaaaagtat gactattatt
     6181 acgaaagaga aacagagtgc tatattcgag gggaaaaagc ctacaacaaa agtgactatt
     6241 ggggtgacaa ggcgaagcga ttaactgcaa attttagagt gtatgaatca gagtttataa
     6301 acttgacctt tcttaatagt gtttttattg attatgtaat tactacgaaa cgcattggaa
     6361 gttattcaag tacctacgca tccttagttc caatgttaaa ctcgatgagc cgtttcttaa
     6421 aagaaagaga aaagaaagag ctacagtata tcaaagaaat catgccggat tttgaaatgc
     6481 acgaatggca aattgcttta tcagattgga aattggcgaa taaggttcgt agaataacac
     6541 cttttcaagc caagcgcttt ttgagagcta tgaaatttgt gcaataaaaa aagccaccag
     6601 acaagttgat ggagactaac acaattacca acagttgtaa ttgtgttagc ttacaaatta
     6661 attatggtgt ttagtcaatc ccaaattgtg aaatggcacc aataattgga aaggaaaaat
     6721 gaaatgtctg aagatttacg agagctgaag ttacgaaaga agcagtatga tcagtatttt
     6781 tccggtttgt tgaataaaca tattgaagaa ggacttgatc atgattttgg aaaagatgaa
     6841 tattgtttat attgcgaacg ttacaatgaa aaaatgtctg aatatctaca gctgaaaaaa
     6901 gagatacaga aattagagaa aaaagtacat gcttggaagt ataaagattc catgtctggt
     6961 tatgttgaat ctcaaaataa gccaagaaaa aagaagcaag cgtatgttct aaagatggta
     7021 aatggggaag agttaattgt attagcgtac agtgaagaag aggttcgaga actcgttgaa
     7081 acacctgtca ggaaaaaggt tgaaacaata aatgagatag acgacagcca atacaatagc
     7141 atctatattg aaacaagtca tggtggatat aagaaattag caactttcct tagaagttct
     7201 aaagttggga taatttctaa aaaagacata agtgctgtta ccgatagttt ttgtacttta
     7261 tctgttccta aagcttattt ccaatacgat agtaaaagat ttgaacgctt agagaaattc
     7321 tgtagaccgt atactgtatt gaaaactaaa aaatttaata aaactaaaag agtttctata
     7381 cagttaccga ctggtcaaat ctcattaggt gaaaaagact atctgataaa agtaggaaaa
     7441 gagttaatta taaaagtacc ggaacactta tttaaagaaa attttgagat acgtgaaacc
     7501 ttggtatgga aagaagaaaa atagtttgga agacatagaa tggtataagg ggaaattgaa
     7561 atggcagtaa ataaaatatt gcaggtacta atcatagacg aatttggcgg aggagaatgg
     7621 tttgatgtgc agtccataga ctttgaagaa gaagtcgtag ttatagaaaa tatttacggc
     7681 gaagtatatt catattcgct tgaaggtgag aatattgagg atttacggtt gattggtgag
     7741 tagaaaaaaa cagaaatatc aaaatggaga taaaaattaa tagcatgtaa agatagtcat
     7801 gcttgggtta aaaatgttaa aattcaaaaa aatgagtcag aagggataaa agtatgaaaa
     7861 gagaggaata caaacaaaga ttaaacgaac tgcttgaaga ggacgaaaca ttaactcatg
     7921 gttcgcctga tgaaattcta tacatgattg ataatatggt gatctttggt ggatatgaac
     7981 ttggtaatag gtcagttgat cataatattt tagaatttga tgatgtatca tgggaagaaa
     8041 ttcttgattg gggaattttg gcggtacctg aaactaaaac ctatattagt gacactatgg
     8101 ttcctttttt tgaggaattg gattataaaa gacttcccaa aaatgaaaat catatactgg
     8161 gaggaaacta acaatgttaa gtgacttatt tattttactg atctatctag gtgtaagtga
     8221 aattattgcg tattttttgt ttgtcattgc taattatggt atttccagtg caatcgttca
     8281 aatgtttaga ttaccagtaa gtgaactaaa aaaatgatga gattatcagc atatctttta
     8341 atgtttttga atgtggtcac gttaatcttt gtagtaatcg gtgtgacttt cgatttaatg
     8401 gaatagtggg aaatctataa tggaaaaaag agacacgaat gaacatattt tttgaatcaa
     8461 tattgttgat ggtttttgcg atcagcgctt accagaaatc taaaaaagag taatgattgt
     8521 ttaatcatca ctcttttttt gtagggaaat tgaaatatgt ggagcttgta ttggtttagt
     8581 taggattgct tggctataag gagctagttc tttatcaaaa ccatcaaagt aggttttcag
     8641 tagcgattta actgatcgct catcttgttt gatttggata atttcagcat ccaaatcggt
     8701 tgtgagatca ggagcttttg atgcgattac ctgatttttt ttatcttcca actctcttag
     8761 gctgtttaac aagttgtaaa aagaatcatt ttgattgttc attccaacgt gagttgcaat
     8821 tttaacagaa gtttcgtccg tattgtcatc aaaagaactc attggaatat caagtagtaa
     8881 cgaggatgtt tcttttactg actttttctt tacagcattt tttgtttttc catctttttt
     8941 gctctttact cgagatgttt ttggagctag tttaggtaaa tcacttgttt tatcagtcat
     9001 ttattagctt ttcctttcta ttaatttatg ttattatatt atcataataa cataaaaaag
     9061 tacacttttt gggggcgtag ttattgaaaa aatctaagag aaagattgcc acattgctta
     9121 tgttaatggt gcaatttaca gggattgcaa tgagtgtaat cacaataaat ccgcaagtga
     9181 tacatgctga tgggatagct acttatgatg cacaatttaa ctgtactact gaaggtagtg
     9241 ggtcaggaac agatagcgat ggaaacgata ctggaagtgg cgctgtaaca ggtgatgtag
     9301 ccaaaaatat tgaagatatt tacaaataca tgaatggaga atatggctta tcaggtgcta
     9361 gtattgcagg tattttaggt aactggaaac aagaatcagg tattgatcct cgagcagtag
     9421 aagggtatta cggagatgga cagagagaag ttgacttcat caaacaattt actgatggtc
     9481 tgatgggtat tggctatggt caatggacag ctgggcgaca tacagcatta gttgattggt
     9541 ctaagaaaaa taatggcggt tcttggtgga ctacagaagc ccaattggac tttatgttca
     9601 aaggtgatgg gggattcgta ggaatattaa aagattatgc tttaaatgct tctgatgatg
     9661 tcgtagaaaa tgcagtaact tttcataaaa actgggaaat ttcagctgat tccgaatcag
     9721 ctgtaatagc tgtacgtggt gaaaatgcaa aagaaatctg gaagtacatg aaaagcaagg
     9781 gtatggacgg tgcaaaagat gaaagtaaga tcaataaaat atccggaggt tcaagtaccg
     9841 gagatggatc gaataattca tcaagttcag catcaggaac agtagaagat gcctgtgcaa
     9901 ctacggatga cgctaattct ggttctggat caattgggaa agaaggaaaa attaacggta
     9961 aaaaaggtca agtaatagcg aatatgactt atgaagaagc tttgaaaaaa tatgggaaag
    10021 cgatcacgct accaaaattc aaagaaccag attggtcaac ttcacctttt aacggaatat
    10081 atcaaggtca gtgtacagag ttgacgtggg cttatatgag ccaattatac gatgggaatc
    10141 aaccgactac tggaaatggt ggttatgtat gggaaagcta caaatcggca ggagcaaaaa
    10201 taacgaacaa accaatcgta ggttatgggt ttagtgcttc ggatggatgg ttatgggctg
    10261 ttggagatgc agggcatact ggtgtagtta tagcggtgtt tgaagatgga tcatttctat
    10321 gtgctaactt taatgttcca ccaacccaag ctccaacaag gggagtttcg gtaacgttga
    10381 ttgatggagt ggacggatca gataatgtgc atttctttag cggtgtagga aaagctaaat
    10441 ttgaaatcga aaaataaatt ggaatgaagg acgatataga tgaatagtaa agtgaaagat
    10501 acaattatca tttctttgct ttctggtatg ttattagcac tgcttgttgg aatcctattc
    10561 ttcaataagc agaatcagga catagaagct gaaaaacaca gtgtgcatat tgaagataag
    10621 ctgacattct ttaaagaggt taaaccaaat gaggtggatg gattacggaa agatttagaa
    10681 aatgatattc aagaaatgtt gaatggatca gttcaaaata agtttgcaga tcaatcaggt
    10741 gaagaatttt tgagaaaact atttattgta acaggtaaac caccaattac tgataagagt
    10801 agtgagaaag aaaaaatcga gtactataag ccttttactt ttaagctaac aaactttgga
    10861 attaagacat ctagtagtgg gttgtatgaa gcattgaccg attttgaagt tcaatacaat
    10921 ggaaaaaaag tgtatgatac cacattaacc atcacactaa ataatgataa gaaagtgact
    10981 gggggtgctt tttatgcaaa atcatgaaat cactactgct aaaaaggcga tcggaatact
    11041 ttccataatt gtgatccttt gttgtgtggt actatttcaa aagaaaaatg cttatgataa
    11101 taatgtcaaa gaattggaag accttaaaag taaacaaatc gaagtagtca agaataacca
    11161 gaaagtgaaa gctatgcagg aagctcaata caaagaattg ggaaatgctg aaattaagga
    11221 acaaagtagg aaattcttta atttatttta caactggaat tcatgggaaa aatatacgaa
    11281 gaatatgaag gaaatacaaa aagaatttcc tcagatagta aagtctagtc ctgttgatat
    11341 ttctggcacg atggtaggca ctggtaatag tccaatatct agctatactg ctgattacta
    11401 tacaacaaat caaaaaggta aaatggttga attgattaca cagacaagaa atactccaac
    11461 aacagcaaca acaacaattt ggcgtggagt aacaagcata ggagaaaatc aattgtttta
    11521 tttggaggat ttagatagct atgtaagagc agagtaaaat aaaaaaagag ctaacggtac
    11581 caacgttagc tctttgtgct gacgatgata acaaaaccta ccgaaaaagg attgtttcat
    11641 cacaaacaca aacagaaaag aattgataaa aaacccgaac ctcagaattt ttattaatca
    11701 attcatttag attataataa attgagctga acattacaat acgtatatgt tgtaaaacgt
    11761 aaaaaaagtc aaaagtaaat actttcttaa aaagtcaaat aaaactatta aagacaatat
    11821 atatggttta tttgacataa gaaaatggtc tagatgataa ttgtaatata gaattgtaga
    11881 cttaaataaa gcataaaata ccttttatga taaggtatag ctcttaattt taagcagttt
    11941 tagatataag attgaaagta aagtggggga gaataataat ggcagatcat aaattagaaa
    12001 agttattaga agaaacgtgg aatccaaaag aatttagtga attttttatg gagaattttg
    12061 aaactgactt agcagtgatt gttaaagatg ctcttagaga gcaagggtat cctgaaactg
    12121 caaactatat aaacattaat tttactttgt atactgaaaa taaaggaact tgggattttt
    12181 gggctacttt agcaaataag gagttaagtg ataaatcaga cacaggaatt cgtaactttt
    12241 ttgaatcaaa cagagatgat tatatgtatg ctaacaatca aaataaattg aactttagag
    12301 tagagtttga tgaaacacct gaagaattta tagaaagaca accgcctaaa gaaaatgtag
    12361 caaaggtatt ggaagatcgt tggaattcag atgaaatagt ttcaacaatt agcgaactag
    12421 gcggacaata tgaaccgcta gtagaagcgg ttagagaaga gctaagactg aataagtttc
    12481 ctgatgttca aaatattgat gtttctcaaa ttgaaataaa cgtcaagatc acgaataaac
    12541 ttgattatgg tagttgggca gatatagcat tagaaaaata catctactca acgttaaaag
    12601 agtttatcga aaatagaatg gatatcatgt atctacagca tccacagtat ttaaactttg
    12661 gtgttgaaat agctacacct cttgaagaat ggaaaatgga gcaagggctt gactagcctt
    12721 tgctccattt ttttatttta caaatgaata cagttcgtcg tttttaccaa gaatgattgt
    12781 ttgcccaact ttgagattgt caggtgatcc ttcagcggtt tgtaaggaga acgttccatt
    12841 gaacttatcg gataccgtta taggtagctt taattctgat ccttcctcat ctttgagaat
    12901 tgctgttttt cgttcatcaa aataaacagt ctttattaaa taatttttcg tagtgatctc
    12961 agcagatttg tctttttttg tagtagtacc agtttcttta gaattagagg tattttctac
    13021 gactattcca tctttggcat ttgagcttgt gtttatttga ttgaatattt tcagcgacaa
    13081 gataacgcta agaatagtca atatagaaac aagtgtaatt aatactgata atttcctttt
    13141 tttgttcatt ttttcttaac atcctttttt gttttgatta gttagatatt ccttatctaa
    13201 caagcctttt taggctatta aatgaagata aaccccaaaa aaatattgca attatgacag
    13261 tgcaatggta ctattattat acaataaatt ttgcgaaagg ggtaggtcta aatcgcaaac
    13321 aaaaagaaat tgaacctaga caaatacaac ttgtataccg caagggaagc aagtttgatt
    13381 ttgggtaaag gtagaaatta tgttacaaac ataatcaact attatccaaa tcgtttgcct
    13441 aaaggttcgt tcaagaaagt gggtgacaaa acctatatta taacggaaga aggaatagaa
    13501 gcattgcgat cacaaacaga aaataaaaac aaaaagaagg aatgaatatg aagagaaatt
    13561 cgcttggtat ggttttattg gcatcagctg tacttgcggg gttatcagta ccagtaacct
    13621 cacatgctga tgaatggact gcaaacacac cggatcaaat ccaaattaca acaggacaat
    13681 caagttatgc gttcaaaaaa ggagatacta tttggtcaac tgtagagaag cttaaaagct
    13741 taggttacaa gactactgtt gctgagtttg ccgaattgaa caatgttgat ttggcaaaag
    13801 gagaacaacg gactatcccg attggacgaa ctttcaacat cagtaaagat acattaacag
    13861 ttaaggaagc agatggcgaa gtagttagtc aaactgttca aaaagataaa cctgatccta
    13921 ttgaaaatac ggagcaagag aacaaaaaat ccgagactac aaaagaatcg gaaccaacag
    13981 caaacacttc tgataagaca gaaacaagtg cttcaagtgt tgatggagca agttcatcta
    14041 atacaaataa ttctacaagt gagaaagaag catcacaggg aacaagtgaa agtagcacta
    14101 cggaagctga tactggaaca tcaacaaata actcacaatc aacatcaaat gatgcaaatg
    14161 aatcaaccag tacatcttct agtgatcaat cacaaacagg ttcgagtgat gaaactgaca
    14221 gttctacttc accaagtaaa cccgaacaac caactaatgt tgtgctaggc gttccattta
    14281 ttagtcaagg taacacaatg ctttgcgaag gaacatcttt gctagaagcg ttacattata
    14341 aaggtgttgc aacaaatcaa gatttaatga cttttgttaa atcaatgcca ctatctcctg
    14401 acaataatcc aaataatggt ttttctggtg aatggagaca caatgtggat ggaacttatc
    14461 aaggtatgaa tccggctcct gtagtttcat ggggtgttgc taatggcgga aatgttgtag
    14521 atatttctgg ttcatcggta caaggattaa aagatgagat tgtaaatgga aatcctgtag
    14581 tagcgtgggt aacttatgcc tttgaacctg ctcaacataa accaatgttt tggggcgatg
    14641 ctatttggaa tcgacatgta gtagccgttg atggatttaa agacggtttt taccatgttg
    14701 tcgatcctgt gtttggggca agttggatta atgcaggatc atttgaaaca gcatataacg
    14761 tatctcaatt agcggtttcg gtacgttaat aaaagatatc gtttatatac taagtagtta
    14821 tatagtcttt atttgtcctt gcttaactac ttagaaaact tgttgctcat ggtgggtaga
    14881 actaaaaggg gtgatgctat gaatactaga gcgttgaaac agcttgcttt caacgaaaaa
    14941 aatgattaat aggtgtggtg attttaactt aggaatgggc gcgaaggtca ttaccacacc
    15001 gacatatcgt ggctcatttt cttcgcctaa ctggtataaa gaaggagtaa cgagagatga
    15061 agcatgaaaa aacgataatt ttaaaatcaa aaataattta ttgttttgcg actttagtaa
    15121 tgatgttatc tacgcttata atgccttttt cagcaataag agctgaagca gatgtcacaa
    15181 aaccgacaag tgcaaaattt atagaaactg gcgtaaaaac agacggttat attagggtta
    15241 atatgccgaa tcatccaaat gaatggatga tttcaagtca atttaaagat tctcatggaa
    15301 atattggata ttgtatggat tcagaattgc catccccaac aggaagtgga gccggaagct
    15361 tgaagtacaa gggagctggc agtgatgagt tctatcgtat gtttaaaggt ggttttccat
    15421 caaaaacagc aaaggaactg ggtgcaggaa atgatacaga agcatggtat gcaacacaat
    15481 tagtttcatg ggttttagcc ggaaacttta aagtttcaca gattgtatgg tcacatccaa
    15541 accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt tgaaaaaatt tatgactatg
    15601 ctaagaatgg aaaagatacg cccaacactg agttttctat cacagcttct aagacagctg
    15661 acgaaggaaa atatcacact ttcacatata aaacagcatc aaataagact ggtaatgcga
    15721 agctcacttt tacatcagca aaaccagctg gtatgaagat ttatgatgca gatggtaaag
    15781 aaattactaa caacactgtg aaattaaatt caagttttac tattaaagta cctgtgacta
    15841 caccaagtgg tactttgtca tttaaaggta ctgctaatgt tagcacaacc aatcctttta
    15901 cttttgatgg acgaggtgtg tatcaagatg ccgtagttat gatcactact tcagaaacaa
    15961 aggactctaa atctttgagt gctaaatgga caagagctgt tggtcaggta acagttaaga
    16021 aagttgatca agatggaaat gctttaaaag gagcggagtt tactctaact gacaataaca
    16081 atgctaaatc tgttaagaca accggagatg atgggttagc tacgtttgat ttgcaacaag
    16141 gcttaacata cagcttagaa gaaacaaaag ttcctgaagg ttatcaaggt tcatttaaaa
    16201 aggatggtat tactgttaat accgatggac aagaatttag ttacactgct acgaacacaa
    16261 ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga tggaaaacca ttagctaatg
    16321 ctgaatttac cttgacggat gattcatccc aaacacaagc agaaaaaaca gatgaaaacg
    16381 gacttgcaag cttcgatatt gtagcaaaca agacctacag ccttgaagag accggagtac
    16441 caactggtta tacaggcgta ttcaagcaag aaggaattac cgttaaagat gatggacaaa
    16501 cttttgaata tacagctaca aacactttga aaaaaggttt agtaaaagtt cataaagtcg
    16561 atcaagatgg aaaacctttg gcaaatgcag agttcacttt aaccgatgaa aaaggtgcaa
    16621 cacaagtaga aaaaacagat gaaaatggac ttgcaagctt caatattgta gcaaacaaga
    16681 cgtatagcct agaagaaacc ggagtaccag taggttacac aggtgcattt aagcaagagg
    16741 gtatcaccgt taaagacgat gggcaaactt ttgaatatac agctacaaat aatttgaaaa
    16801 aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa acctttagca gatgcagaat
    16861 tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa gacggatgca actggtctgg
    16921 ctacgttcaa cattattgcg aacaaaactt acaacttaga agaaacaaaa gtaccaacag
    16981 gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa agatgatgga caagtctttg
    17041 aatatacagc taaaaacact ttgaaaaaag gtgtgatcaa agttcataaa gtcgatgatc
    17101 aagggaaagc attagcaaac gcagaattca ctttgacgga cgacaaaggt gtaaaagttg
    17161 tacaaaaaac ggacagaaat ggcttggcaa catttaatat tgttgccaac aaaacgtaca
    17221 aaatcgaaga aacacaaaac cctaatggtt atacagggaa atggtctaaa gaaggcatca
    17281 ccgttgctga cgatggacaa acctttgaat atacagtaat taatcacctt gtacctgaaa
    17341 cagtaagaac aggcggaatc ggtacatcag ccttccttct tgtatttcta attgcaggat
    17401 tgagtatggt gctactgtat tatcgagaac agcacaaagc aaaataaaaa agtgttaatg
    17461 gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa atcaaaaaaa attttttcaa
    17521 atttaatgct atttggtact ttgctaggag tgggattggg cgcaagctca atctcttcta
    17581 gccttggact aggggggcaa gtagcatatg cacaacaaaa ctataattat caaggaactg
    17641 gaacgattca tatccataaa gtaacaaata ctaatgggga tgggaaccct gtctcagata
    17701 ttgataacaa agggaaagaa atagctttac caaatggtgt aaaacctctt gaaggcgttg
    17761 agttctctta tgtgaaactt actaaagatg aagctaaatc cttgggatgg acggatagtg
    17821 atactacaaa aggtcaacat attgatgatg ctaaagcacg attattacta aacaaaaaat
    17881 acagtgaagt aagtagaatg aagactgatt cacaagggaa tatagacata cctatacaag
    17941 taaatacaga cgatttaaca gataacttgt atttgattgt tgaaacagga acagtcaaag
    18001 gtgtagttga agaagcgtat cctatgattg ttaacgtacc tgaactaaac ggatcagatg
    18061 atcaatactt taattatgat gtacatgtct atcctaaaaa ctatttccaa ttaaactcag
    18121 cagagcttga taaaaaagac gaagatggaa aggctcttgc cggaactgtt tttgaattgc
    18181 gaaaagatga tggatctgat caggttgttg gaacttataa aacagacagc acaggtaaat
    18241 taactgtgga tggtttggaa ttaggtgact attatttcct agaaaccgat ttgggaacag
    18301 atgctaatag aacaacctat ttactagatg gtaaaaaatt caaatttagc gttaaacgtg
    18361 gggagactgc taaagtagtt cttgatgtta ccaactacaa acgacctgta attactaagg
    18421 taattgataa agaaaccgtt gatactggcg aagatgttca atatacattg aaaccatcaa
    18481 ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa agatgttcta ggtgcagagt
    18541 taacttatga ttcatcagaa gtgatcgaag gaatgacttt agatactgat tacagtatcg
    18601 aacgtgacgg acaaacgaca aactatgtct ttactgaatc aggtatggat aagcttcaga
    18661 aacaatttga tgcaggggat cgcttcttta caattttgat gaacgctcat actaatagta
    18721 aagctaaaat ggatacacca cttaagaatg atgtttcaac gatctacaac aatggatatg
    18781 aacccgaaga cgaaacacca gttgatcacg cttatacaat cattggtgat caccaattca
    18841 ttaaaacaga cggagataca agcaaagcat tgagtggagc aaaatttgta gtgaagaaca
    18901 aaattgatcc gaataaagtt acttacctaa aacaagatcc aacaacgaaa gctgttactt
    18961 ggattgcaga taaatctaaa gctactgaat tcgtttccgg atcagacggt aagttccaaa
    19021 ttgaaggttt gcaatatacc tatgatgtag attttgataa acaaggtaat ccaacaatca
    19081 cagataaaaa aatcaatgat tatgctcttg aagaagttgt tgctccttca ggatatacac
    19141 caactagaaa cgatgttcca tttgtggtat cgaatgatga tgcttctagc gaagtaacag
    19201 acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg tggaattgga acagtagtat
    19261 ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt tgtctacaaa cgagaacaac
    19321 acaaagctta atgaaataaa attccctaga gtgtttttgg ctctagggtt tctttttaaa
    19381 aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat atcaagttga tcatcttaaa
    19441 aattgggatg ttgctatgtt taggagtagg tctatatcct gtaattactc aggcttatac
    19501 ctactaccaa actcaacaaa cactaaactc ctataaggaa aaatatgatg aagcttctga
    19561 aaaggagaat gaagaagtct tcaagagata ttcagaagtt aataggaaac gaaaagacac
    19621 agaacaaacc tcagtcacga ttcctgaaga taataacaat attccgtcaa atggaaatct
    19681 tcagaaggat aaattaggtg aattagtggg tgaaattctt ataccaaaga ttaatgtgga
    19741 tttgccaatt tatgatggtg taaacgattt ccaactatca gcaggagcag gaatattaac
    19801 cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag ctcattcagg
    19861 cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg ataaattttt
    19921 tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa caattctacc
    19981 aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac ttatgacatg
    20041 tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca taccttacca
    20101 aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg cgcagatgaa
    20161 ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag tcattattaa
    20221 aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta ctttttttga
    20281 ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat catcaattca
    20341 aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa actccttcaa
    20401 aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac caaggattag
    20461 acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac ggtggatcat
    20521 ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt ttttcaaaaa
    20581 atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat gtctatattc
    20641 caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat ttatatagag
    20701 gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat caaacagtaa
    20761 tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat caattagtag
    20821 ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg gttttccgaa
    20881 cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct gatttagata
    20941 tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta ttagttagaa
    21001 gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc aataccgcat
    21061 acgctgatgc cattgatagc gagtccgttt cagataagac agaaactgtt acatcttcta
    21121 atgatttatt tgaggatgtt gatcgtgcca acaaggaatt agatggaaat aatgatctta
    21181 atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa aaggcaagtt
    21241 caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt tggactgtaa
    21301 taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt cgaaaagcat
    21361 aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta ttattgatct
    21421 cttttttatt ttgaatagag ctagattcac ttttaaataa aatcaaaact gatcttaatt
    21481 ctaaagaaaa agtcaagaaa aaaatacaac aaattttttt atcagaatat ttatcagaat
    21541 attattagaa aagataaact caaaagctta atttaatagg gttattggtt gtatttagaa
    21601 ataatgtatg gaaaagctat aaatcaatag ataagatgta gaaaatctta tttgtgaatg
    21661 aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtgggaa tatcaaaaac
    21721 agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag aatttgttga
    21781 atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acaatgaaaa aagactattt
    21841 gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg atgtagtatg
    21901 tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa ttggagggga
    21961 tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct atggttatct
    22021 aataggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa cacctgaaga
    22081 aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca aatgacagag aaaaagtaaa
    22141 attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag aaatcaatga
    22201 agacagcaac tattttcctg aattgaaggt atatcaaaat gacgaagatt tctttaatga
    22261 aatgtttagt aatagtccat gggaagcagt gagtaaaaca aatttaggag aatggaactt
    22321 tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt cagaaagtga
    22381 gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt taaatctatt
    22441 agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag aagagctata
    22501 gggattattt caacataaac acaagcagaa aactactaaa caaaacgtac catgcaaaaa
    22561 gtcggttaaa ccggcttttt gctctattat gagaggagat caaaccatga gtgcaacaac
    22621 cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa acgtagaaga
    22681 ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag aagaaaatat
    22741 cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta tcgcatcgaa
    22801 actaagtcaa gttgccgagg ataaatcgtg gaatgaatta gttagttttt ccgaaaaaat
    22861 ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc aatacaagaa
    22921 aattgacgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg aaggtataga
    22981 taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg atgatcttga
    23041 aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag atacagaggt
    23101 gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata tcgtaaaaca
    23161 agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag ttgtaaagta
    23221 tctagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg aagaaactgt
    23281 tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt tgagtgatca
    23341 aaactcggtt aattggagtg tacgattatt aattgatcaa aatgatattg aataaaatag
    23401 agctgaaaag ctctattttt tttgttaaaa aaactagcta ttttattaaa aataatttgg
    23461 ttcaaatttt atttttttaa cttgataatt tcttgccatc cttttttaaa ttggctatat
    23521 atcaaggttt atagtgggaa taaataaaaa tacatttgac aaaatttaca atatagttta
    23581 ttatatattt atcttattat gataacacaa taaaaggaat ggtggtatga aaaagaagaa
    23641 gtttagtcga tcgttgttag gaattttggt tttagttgcg gttggtctta gtggcaaggg
    23701 ttactttcag aataacagca ctcaggaaca tgattttcag atcaaaagta gtgttcagat
    23761 aggtaataaa gcagacagtg aatcaaaaga atttttaggg atacgttatg atccaaacaa
    23821 atatccaaat aatatcgtaa acattaatga tgggaaatat acactgacaa aagaagacca
    23881 tgaagcatta tcagaaaaag gcagtcaaac cttttgggta aattatgcag atttagatca
    23941 acttggaaga gcaggagatg ttgaagcgtt gatcacctat gatgctgtaa attctcactc
    24001 atctaaaaat gtggaacgtc catctttcaa aagttctgtc catattgcag gagaatacaa
    24061 ggatggagta tttgatcaag agagagatac ttgggtcggc aaagattcaa acaatgaaat
    24121 cactcagcta gaaggctata aaggttatct ctataacaaa agtcattcat tagcatggtc
    24181 gctaggagga gatatggagg tacacaattt aacacttgga acgagagcgc aaaatgttgg
    24241 gaaaaatgat cagaatggag ggatggctta ctcagaaacg attgtacgtg atgcaatcta
    24301 taaaaataag aatgaaaaag tattgtataa agttgatcca atttataaag ggaatgaatt
    24361 agttcctcgt ggttctcatg tcgaagcttt ctcagttaac gataatggaa aaacggtaaa
    24421 ccttaatgtt tatgtattta acaatcaaaa aggaatcaac atcaactatc aaaatggtac
    24481 ttgggtaaga gattagaaat tgagaaggtg aagaattgga aacaataagc atcaatggat
    24541 tagataaatc agttgcaaat cgaataaaaa aaggaaatgg ctactatctt ctgaagaagg
    24601 gtaaatattc agttgctaga gagttagaaa caatgagtat cgtgtatgta gctaagaatc
    24661 attttgacgt agaacaggct atttttgatc cagattctga aatagggaaa tgtgttgaat
    24721 tatcagagaa aactgttagt acaaaagggg cgatggtcat tgagagagaa atgttagacg
    24781 aaatcagagt aatcattcct aaagatacgc agatgtgtga ggacagcata agtgacgatg
    24841 aaccaaaatc agaaagaaaa taaggagata ttcagatgag cgaaaaatcc attgaattaa
    24901 aggaatataa gcgaagcgat ggagaaaaat attatcctgt agccagtttt tctaaatcag
    24961 tatatctggt tggacgtgca ttggtagttg gtgcaattgg catatatatt ttgcattggg
    25021 taacaaaggt tgtacaactg cttgtagata ccttagtaag tattcttgtc tcgttaaatg
    25081 atgttaacgg aacagatgat ttaggattaa aagagatgtt taaagagtta ctcaatgcgt
    25141 tttttcccaa ttatttaagt tattcatata atgtacaaag cttttttgca aagctacact
    25201 tggtagacat ctctatttta gtattagaag caggaacggt attctttgtt ggatatttgg
    25261 ttactttgat gttcaggcat cactatggag aacaaagacc attttttgat gatatggaaa
    25321 gtagaaggtt gaaaagagaa gctctagaag ctatggatgt gaagtatgat ccaaacaaga
    25381 ttataggaga tgaaacgaac aacaaaaaaa gaaaacgaaa caaaacgcaa ttagaatccg
    25441 atgtgctatc aacgcttaaa aatatcaaaa tttctgttca tacaagacgt gatctaagtg
    25501 tcaatgaaga tattcgagaa tatatcgtaa aaatcccgca accaaaaggg aagaaagtta
    25561 gagaaacagt tttacgacaa attcaagatt tagggtcgat cctgacaaga gcaacaagcg
    25621 gacgagccaa atttggtgaa aaagaagatg aagtttcagg cgattatttc attttccgag
    25681 catcagtaaa tattgaaaga gaacgtaagg aaagagtaag taccaaaaac aaagaaacgg
    25741 aaagagcaac atcttcaatc gcagtttcaa attcacaatt ttcttttcca atcaatctct
    25801 ttgccgataa ttccaaaaat atccaaaacc aaaccgaaaa agctagaaaa tatgccgaaa
    25861 ctcaaaagca atctttagaa ctttatctat cttctgctga cgtacctgca agtctaacag
    25921 atatgcacgt tgggaataca tctattgaat atttgtttac tttatctcgt accagaaaaa
    25981 ttcaactaag ctcaatgaaa gaagaaattg aaacttactt gcatatatca ggtgtaatcg
    26041 tgcggatcaa cgcagggcgt attcaaatta ctttaccatt aggagaaaat gatcgtattc
    26101 caattgatgt accatcaatg atcaatagcg tgttttagaa gacatcacaa atagaaacag
    26161 aaaatacaaa ctaccagttc acaaacagaa aagtaccgag aaaagtgagg aatagaaatg
    26221 gctatgaaat tattagagaa agtcaaaaag agagccgaaa aagctgaatc tattttgcta
    26281 gataaagaac ctaatataaa gcttaatgga aaattcagtg tcaatatgtt gaacacaaag
    26341 gctaaagacc caacagatgc tatttttgga attaagcctg atggcacaat tgctcatttt
    26401 tgcatcagcg aaatgccaca catgttggtt gcaggaacga caggatcagg gaaatcagta
    26461 ttactgaatg aaattcttgt tacagtaatg tgtcactcaa cgcctgatga attaaaaata
    26521 ggaattgtag acccaaaaag ggttgaattt ggaagataca aaaaattgcc gttcatgttg
    26581 gctgatccaa ttacagatat ggatgaagct tatgattttt ttgaatatct tgtgatcttg
    26641 atgcatgaac gttacaaact gatggaaaaa gcagggttac aaaacatcac gctatataac
    26701 gaatatgccg aaaaaaatgg attagataga tttccctatg tgatcttatt agttgatgaa
    26761 tattcacagt tggttggaac acataaagaa gtagaaggtt tgattgttca attaggacaa
    26821 atggcaaggg cagcaggtat tcatgttatc ttagcaacac aatctcctcg atcaacagtt
    26881 gtcactggta ttatcaaagc caattttccg tcacgtgttt gccttatggt agcaagtgat
    26941 ttagaaagta ggattgtatt agatgaaggc ggtggagaaa gtctaagtcc aaagggagat
    27001 atgcttatta agcttgttga tggaagtatg gttagagcgc agggagctta catcagtaac
    27061 aaagaaattg aaacaatctt caatcacctt agaaatacta tgccagaacc agaatatgtt
    27121 gattaccgag caatcgtagc tgaaactcgt ggtgagggcg gtgaagacga ggattttggt
    27181 ggaattggta aagctcctgt aagaaaatct gctaaaagaa atttgaacac acctttagca
    27241 aatccatcat ctaaagagcc atcaattgaa aagaagagtg cgtttgctat taagcttgaa
    27301 aaaaataaag aagaaaaagc taatcaggga gctaagtcac tagaaaaact tcagcaagaa
    27361 aaattagaaa aactagaaaa agcattatca tcatcaactt ctgaaactca atcttcaaag
    27421 ggtatagata atggacaatc aattacaact aaaaacaagc ctaaaaatag taattcaaat
    27481 ctctcggtaa atgttgttat cacaactgat aaatctgaaa aaaaacaagt gaacggaact
    27541 acttcaccca tcatgcaaaa aatgataaag gcaaaaaaac aggtgcagaa atgattatca
    27601 ttagttcctt tttgagatgg ctaacaccat ataaatatac agatgaaata gttgagaagc
    27661 gagcgaagaa aaaagttaaa aaaacgatgt taactcctaa agaaatgcta gatcgtggaa
    27721 tattgggagt gaaggtttta gaagtaaacc aaccgtttga attggataat gcgagagata
    27781 ggttgcttag agctgaagtt gatgtcgcat acttttatca tgataaaagc aagcatcaaa
    27841 tagctatcaa attcaatcca aaatcggcat ggcgatacca agcaagagat gacctaaagc
    27901 gtaggaagct gaaaccgatg ctctatcctg aaggaaaaga ggcatttgat agaacccata
    27961 ttgttcccat tggatttcat ggtacagagt cagataaccg cctattggta gggtggaaca
    28021 gctcacaaaa tagagttcag atcaataaat ttgagaagaa ggtaagaaaa tttaacgatc
    28081 ggaaaataat actttggttt gccaatattc agaagcaacc agatgggaca gcaaagtggg
    28141 aagcggtcat atttgatgaa gatggcaaaa aagtaatgtc tgaagtatgg catgataaag
    28201 ctaaatttgt ttggcagtag aggtgaaaat tgtgttaact aacctaaaac tatatagaga
    28261 gggatttaac gaaaaagtaa gtatcggtga atctattgaa tatttaggaa agaagtacat
    28321 tattattcgt attcttgaaa ttaaaaaact ttattttagt aaatataata atttagcagt
    28381 tagagttctc gtccaaaagg ttggaaatcc aagtgactat agaagtgatt accataacta
    28441 taaaacagaa tcatctgtaa ttaagcgtta taatcaatca aaaaatgatg agagaatatt
    28501 caaagtagga gaaattatta tttctaagta tgggatagca tacgaaatag tttctattaa
    28561 aaaattttat tatgaatttg ttgatttggt tgtagaatat tctgttcgat tgatcgttcc
    28621 gtggagtaaa gaagaaatta atgaggcatt aaaacatgaa cgaaaaagta cattcaaagt
    28681 tttagaaggt gaaaaaaagt gaaaacagac cttactaggc aagctgagaa gtgcttatgg
    28741 catgaaacaa atcgcatggg tgtatttggt tgtttcgaag tcactatcgg ttgggatggc
    28801 agtgaaagag tagattatat aacttattca gtagataaca cagttcgatg ttatgagatc
    28861 aaagttactt tagctgattt aaaaagcaag gcgaaacaga cttttatcgg tgattataac
    28921 tatttagttg ttactagtga tttgtgggag aaaatgcaac aagacgataa acttatgcgg
    28981 aaatattact atcaaggcgt atacgtgttc aatgaaaaat atcctagcat aataagtgtt
    29041 aagaaagcaa ggaaacaaaa agtatcgcta ggtacaagat caactgtatt agaaagcatg
    29101 gttcgttcta tgaatcgtga agttaagaaa ctctataaag taaaaccatt ttgggagata
    29161 gcagaggtgg aaaaaggatg attcaaacat ttgcaaaaat gagtagaagt gcttggattg
    29221 aaaagctgga aaatgatttg agtgttatgc gattagctcc aaagaaattc aaacgagaga
    29281 ttgcagaaaa agaagcaatt ttgtcggtat tgaatgcggt agaactggac gaaccgaaaa
    29341 agtcagttga tgtacctgaa aatgtagcag agttactaga ttattatcgt aattcgtcag
    29401 atgtcgattt gttagctctg atattaacat ttcacgattg gtattactgt caaaatgaag
    29461 atactcagca tgataaacat gataaagcag ttgactggtt aatagagaat ccagaaaagt
    29521 tcatgcgtgc ttggctagat ggctatgata gcaagaaaaa gccactggaa agtaatgagg
    29581 aaacgtattt aacggcagaa caatatgact atgctagaaa aacaggagat tgggaatctg
    29641 gtaagacttt caatcacaga aaagatcaac caaaaattag tttttcagat atagccgcaa
    29701 ttatcgaagg tatctactat ggacaagccg atgaagagat cttttctata caagatttat
    29761 tagaattttt gaacaaaaat aaggatagct ttgtagaact aactgattat gaggaggata
    29821 cagcaaatgg aaaattatga tccaaatatg cgatggggta cacatacgct taaagtttct
    29881 tttcaacgtt gggattacaa gggatttgtg acttttagaa aagctggaaa ttgtaaagga
    29941 ttagatgtct tagcgttgga tgaagactat ctatatgacc atccattgac ggataatcca
    30001 atcggatttg gattgcttcc tgaagatgat gagggcaatg aatggttcaa aatgattctt
    30061 acaaacgata aaggtgatca attatttgta gaagatactt ggtcatattt gagtgaatat
    30121 attgttagca ttaaaatcat tgattttgta gcagataaag aaaaagagat tggagaaggt
    30181 aaatctaatt actagctaat gaatagcaac tttggaggat aagtcaaaag aataaaaaaa
    30241 gaatactgtg tgtcattgca gggatgtatc tttgcatgat agtatctgcg gttaccattt
    30301 attggttagg aaatcagcaa aacagtttaa ttgttgatct gggggagtta aaggttggat
    30361 tacgaatgac tagatggctt ttattagctc aaacagttgg tttagccata ttgacttgga
    30421 tatgtgtgga aaaaataaca tctaataata atcaagagat tgttgaatca acaaacctaa
    30481 gaataagaat aactgaacaa gcaattgaaa agcaaaaaga caaagaaaaa aaggaaataa
    30541 tgtcaattta tttatctagt ggagcatcat tttttcatga aatagattcg atgaaactga
    30601 gtagtcaaga aatcattgat agatggaaaa acatgcctct aggtggtgag ttaatcgttg
    30661 aagatagtga tggttggtca gcttataaga aggacgagat tgtagctatc acgattttgt
    30721 ttgaatagta atatattacc actagataat ctgaatatgt tgatctgcta gtgacgtacc
    30781 aaatgcgttt aatagaacct tggtcacagg cagaaattga taaagcagta aaattgaacc
    30841 gattatcaaa atttaacgta ttaggaaatg ccttttaaaa atcgcaatac acaaacagaa
    30901 aaataccaaa aaattagcaa tgaacgtagg taagaacatg acaataaata ataataaccc
    30961 taacaaatat aatgctcttg tccaatgcta tatcgaaaac aaaattacaa tgcaagaagt
    31021 cgctgatgaa ttaggcaaat caaaaagcct agtatcacac gactttaagc acaatgtttt
    31081 tccgaacccg attgagctga ggaaaaaatt tcaaaagaga gtttatcaag aaatagggaa
    31141 gtggattgat gaagggaatg caattgaccc tttaattgaa aaatatcctt ttttaaaaag
    31201 taaaaataaa attcaaaggt taattcgtca aggcgatatt caagctaaag aacctgtttt
    31261 gacaaccaat gatttggcta aaataaatct tcataaacag attattgaag actttgaaaa
    31321 aaatctttca acaactgaaa ttttaaaaaa atacaatatt ccgagaggaa agctaacatc
    31381 gactcttttt agagaatatg gagaatcggc agttactaaa gcaaaggaag tagcgaatga
    31441 gagaaaaaaa caaaaatagt agtcgatacc tagtagcgac tgtagttttt gtttgtgtat
    31501 tgattttcct gtttgatttt ttgcagattg gaaaaagcat aattggaaat acattgagtg
    31561 caaaggaaat ggttgcttta ggtggtttgt atgaaggaca tttgttcccg aatatattca
    31621 tttctatcgt ttctcatatt aacttgtacc atttaatttc taatttattg ttcttagtag
    31681 ttcttgaaga agcactgaat gatcaatatg agtggttaac actattgatt gttttaatca
    31741 tatcaggact tctaggaagc ttagttcctt ttctgttaca aagcaacatc gtaagtatgg
    31801 ggcttagtgg aggtattttt ggatggattg ctttatttgc gattgcatac atccgctttg
    31861 atagtaagga acatttatta ctaagtgttt ttatattatt gataatcggc attttactgg
    31921 tatctacttt tgaatcaggc gagacaaacg tcttgtcaca tgtaatcgga tttgtctgtg
    31981 ggatgatctt gggatttatt ctaaagaaca tttcattgat caagcaaaag aattaaaagg
    32041 gagtgctaaa aatgccaaaa aaaaaagaaa agaaaattcc agtgtttttg tctgtctcag
    32101 aaatgttatc acttcaaata ctgttaagaa aaaagattga aacaatagac gaagaagtta
    32161 gaaataaaag ggctatgtac gaacaacgtg aagcgctaag agaacgaatc cactattttg
    32221 atgatataca cactaaaatc tatgaagcac gtacaaataa cgaagacgaa agcaaattag
    32281 gattagctca acaacgaata tttgaatggc taaatacgaa tactgagcaa gaaccgttaa
    32341 gagcaattag taatcttttt gaatttggaa cgaagcattg gtttaacgca gtagaaaagg
    32401 atgctgactt acaagaagcc tttgacacgc taagtaagga tagcaaatcc atgcgagatt
    32461 tattgattac ttatctaaat gagcatgagt ttgaacaaac gaaaaccaaa aagaaaaata
    32521 aagaatagaa agttgtgatg aatcatgcta ttaaccctgt taggttattt atgtttaatt
    32581 cttgcttttt ttggggcaat cgctataatt attggtcaat ttcaaacaaa taatccgatt
    32641 atgcgtaaca taggcattgg aattgtgatt gtaaccttta ttgtgtttaa tgccactaag
    32701 atttttggaa gtaacgataa acaatttggt atatcgggag ttaataatag ttatgctcca
    32761 gattctttgg gaagggtagt aatagatgga acatttgaaa atgtaacgag ttataaaaat
    32821 gtttcgctga cagtgaagga agatggcaag aaagcatcag acgacgttct cgctactatt
    32881 aagccgtctc aatcacaatt tgaagtgttt tatacagtta aagagaatag ccctgtacac
    32941 aagctgacat tggtttttaa cgttgatgga gaagaatact ataaagatgt aaaaataaat
    33001 aaaacatcaa cgaaaaagat tatcgaatca gcgaaaaaag aagtagctac agaagaaagt
    33061 acaactgaat caagttctac tgtcgaagcg gaactaccag ataaaattga ttttgatcct
    33121 gaaaaagtca aatcgtttga atctgtttct ggttctatgg ctgatcggtt taacactgaa
    33181 gtcttcaaag ctatcaaaac tgttaaattg gatactgaaa agcaaatgat taatgttacc
    33241 ttattcaatt cagttgaatc tatggatcag gataaaaaag ttacggttgc aagagaacta
    33301 ggaaaaagag taattggttt gtatcgtggt actgctaaac cagattcaga cggtgcagat
    33361 ttgccagttc atgtagttta tgaagacggt aaagtttttg gagattctac agcaaacgaa
    33421 gcattaacga ttaaattggc taaatagcca gaaagttata ataaacaaat ggtgtcacac
    33481 caataatata taggtgtgac accttggaga tgatagctga tggtagtaaa taaagaaaaa
    33541 gttagaaagt atgtgacttt aaaaatagct tctgttaaat atttagaaaa gcgagctaaa
    33601 gaagaagaaa gagcaagagg taagaaagta acagtttcag agttgttaga agaattgatc
    33661 gagaatgatc aaacaataag acgtgcattt actaataggt ctatcagata acaatataat
    33721 taataaaaaa gaaagagtga tttggtctta gactaaatca ctctttttat ataaagtttc
    33781 tttacactca ttccgaataa tgttaaaatt ctttatatga tatatagttc cttgtcagag
    33841 gaagagataa ccagcaatat tggagggata ataagtggaa aacaataatg atattagaca
    33901 ttttagcatt ggggaaagag ttacaacagt aagtgaatta tcaaaggatt atagagataa
    33961 agagggaacg attatttctc ttaataagaa tcgaagaggc gtttggcaat atgttgttga
    34021 tttaggtgat ggcgttacag ttgagtgtaa agatagtcaa ctgatcagaa ataatggcag
    34081 tcattccatt gaaaaagcaa ttcttgtata cccaaaagta tcaagtaaga tcatgaatga
    34141 ctggttgctt ggaaaatttg gtgagtacaa taaaaaagaa atcgcagaag tgatcggcaa
    34201 atcttatcca acagtattag ggtatctaaa aaagataacg agtaaagaag caaaaattga
    34261 ggatgtttta acagtaagag agcttcaatc attaatcgag tatcggaata aacaaataac
    34321 aaatcctgag taccttagaa aagaagatgg tctattgaaa caattggaac aagtaaataa
    34381 aatccggaag atgcaaaagg tagctccttt aagccttgat acctttaaaa aaatctattt
    34441 gaaagagatc aaaaaagact aatgtggact gtaactttac cgttccaacc tgtatctgca
    34501 ccaagaccaa gtgcaagaag gaatgctgct gatgaaacta atgacgtagc agagaaaagg
    34561 atttcaacct actatgataa aaagtatctt gattatttga aaaaaataaa gagctttatc
    34621 gaagatcaag atttattaga tgatgaattt tactcgattg taaatgatcc tatgggcgca
    34681 attatggaag tggactttta ctaccagata ccaaaaagct ataaaaaagt ctataacatt
    34741 atgaagacaa cagcgccaga tttagataat ttagtaaaaa gtgctatgga tggtattttt
    34801 aacatctcag ctatacgaga tagccatatc acaggcttaa ttgcttttaa atataatgtg
    34861 gcgaacgaag gaaaaacagt tgtgaggatc aaacgatatt cagaatttga gtgggatact
    34921 tctgaaggat tgaacggaga tatatgggaa gctacatttg attttaaacc agtagcaacg
    34981 ccaagaccaa agtctaaatt tagaggaaat gggattatca cttactatcc aaaagagtac
    35041 aacgactatt tggaaaatat caagaatacg cttaaagaga aagatttggt aaatgatcaa
    35101 ctgaaaaagg taatgaatgc tcctatgggg gttatagctc agatagatta tttttaccaa
    35161 gtgagaaaag ataagaaaaa gcttgattca ctgatgcgta cttcacagcc ggatattgat
    35221 aatttagtca aaggaacatt ggacggaatt tttaacaagg aaataggcat caaagatagc
    35281 agggttgtag ggttaattgc ttttaaatac aatacttttg aaaagtcgaa aacaaacgtg
    35341 agactacagg agatgggctg atgaataata atgaaaaaat tcttcatgta cttgatagct
    35401 ttgaaataat tcaagaagaa ctaaaaaaat atcgggatgt tttggaacaa agatatgatt
    35461 ttgtcaatca gcaaaagagt aatcacatgg atttcattct caatatgaat gacttgaaga
    35521 agaaattagt tgaacgaaaa gaacaagaaa agttgatcaa agcttacttt gaactcggtg
    35581 aaaaagaagt taaaaatgca atggaactga atgaagagcg cagggtttta gatcagctgt
    35641 tagaacaatt attggtgatg tttcaaaaag gacgaataga tgaggattta attgaagaag
    35701 gattgagaaa atatccttca aactcaggta tcggaatagt gctgaaagcg attgatgaag
    35761 aagaaattga agctttcata ccgccagagg attttgaatc ggcaatggaa tatataaaat
    35821 attatagcca agggatcact gcttttagag agtttgatcc tgaagatgtt attcatgact
    35881 tgaacaacct aaaagaatgg tgtgaaagtt atggagtcga tgattccgga ttggactact
    35941 tgatcagtat tatggaaatc gaagaagaga tgccggataa gcctgatcca acagatattt
    36001 tagaattgat ccatgaagct agaaatccaa ttgcctatat ttctagagga tatactgtat
    36061 tggaatacta taagccttat atatcagcga tgaaccattt aagacgtgta ttaagggaaa
    36121 aacgtgaata ccgctcagtg ttgaatgcaa cgaatagatt agaaaaagct gtttcagagc
    36181 tagatgctta ttatcgtgaa cattacctgc aagcaggtgg tatgccaaga aatacgaaag
    36241 caaatatcag taggtatata aaaaaagctg agtaggcgga gttagcccat aaaatggcaa
    36301 aaggtaacct gaaaggggca ttttgaatga aaagggatgt ttttgaattg tacgtattag
    36361 aacgtctaga agacggcatg atggcacaag agataattga tgaaattgat gatatgatca
    36421 gcagatttgg tcaattggag ttgaatgatg gaaacaaaaa ttcggtagtg ctaaaacaat
    36481 tttggttgaa caatttttcg tataaggatt ttatcaaaga aaaggcaaat acattgtact
    36541 gactaaattc acgaggagca gtgatgagag cttattgcta agagcagtta ggctcttttt
    36601 tgtttgtgta aaattatttg ttttataata cacatttata cacaaaaaaa tattgacaga
    36661 tagtacacaa atgtggtagg atatatttgt aagcaagtta gattaacaag ttaaaggaag
    36721 agcagttatg aaaaaagaag aactaataga tatatttcaa attgttgaac gagcaaataa
    36781 tatgggaatt atgttttttg atcgtatttc tttaaagatg
//
LOCUS       DASJPH010000017        35314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJPH010000017 DASJPH010000000
VERSION     DASJPH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.017"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            361..2061
                     /locus_tag="U3995_000791"
     CDS             361..2061
                     /locus_tag="U3995_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796134.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2163..2996)
                     /locus_tag="U3995_000792"
     CDS             complement(2163..2996)
                     /locus_tag="U3995_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1796135.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3300..5465)
                     /locus_tag="U3995_000793"
     CDS             complement(3300..5465)
                     /locus_tag="U3995_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1796136.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5725..8019
                     /locus_tag="U3995_000794"
     CDS             5725..8019
                     /locus_tag="U3995_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1796137.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            8012..8677
                     /gene="pgmB"
                     /locus_tag="U3995_000795"
     CDS             8012..8677
                     /gene="pgmB"
                     /locus_tag="U3995_000795"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1796138.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8670..9677
                     /locus_tag="U3995_000796"
     CDS             8670..9677
                     /locus_tag="U3995_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1796139.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9699..10718
                     /locus_tag="U3995_000797"
     CDS             9699..10718
                     /locus_tag="U3995_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796140.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10999..12366)
                     /gene="nhaC"
                     /locus_tag="U3995_000798"
     CDS             complement(10999..12366)
                     /gene="nhaC"
                     /locus_tag="U3995_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1796141.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(12475..13896)
                     /locus_tag="U3995_000799"
     CDS             complement(12475..13896)
                     /locus_tag="U3995_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796142.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(14139..16016)
                     /gene="tdc"
                     /locus_tag="U3995_000800"
     CDS             complement(14139..16016)
                     /gene="tdc"
                     /locus_tag="U3995_000800"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1796143.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(16307..17563)
                     /locus_tag="U3995_000801"
     CDS             complement(16307..17563)
                     /locus_tag="U3995_000801"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1796144.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(17675..17880)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18143..19444
                     /locus_tag="U3995_000802"
     CDS             18143..19444
                     /locus_tag="U3995_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1796145.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(19513..20112)
                     /locus_tag="U3995_000803"
     CDS             complement(19513..20112)
                     /locus_tag="U3995_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1796146.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            20366..21247
                     /locus_tag="U3995_000804"
     CDS             20366..21247
                     /locus_tag="U3995_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796147.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            21260..22870
                     /locus_tag="U3995_000805"
     CDS             21260..22870
                     /locus_tag="U3995_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1796148.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(22947..23606)
                     /locus_tag="U3995_000806"
     CDS             complement(22947..23606)
                     /locus_tag="U3995_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1796149.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(23889..24554)
                     /locus_tag="U3995_000807"
     CDS             complement(23889..24554)
                     /locus_tag="U3995_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1796150.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(24701..25306)
                     /gene="dhaL"
                     /locus_tag="U3995_000808"
     CDS             complement(24701..25306)
                     /gene="dhaL"
                     /locus_tag="U3995_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1796151.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(25334..26344)
                     /gene="dhaK"
                     /locus_tag="U3995_000809"
     CDS             complement(25334..26344)
                     /gene="dhaK"
                     /locus_tag="U3995_000809"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1796152.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(26319..26765)
                     /gene="dhaM"
                     /locus_tag="U3995_000810"
     CDS             complement(26319..26765)
                     /gene="dhaM"
                     /locus_tag="U3995_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1796153.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            26868..27188
                     /locus_tag="U3995_000811"
     CDS             26868..27188
                     /locus_tag="U3995_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1796154.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(27300..28169)
                     /locus_tag="U3995_000812"
     CDS             complement(27300..28169)
                     /locus_tag="U3995_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1796155.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(28265..29569)
                     /locus_tag="U3995_000813"
     CDS             complement(28265..29569)
                     /locus_tag="U3995_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1796156.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(29566..30075)
                     /locus_tag="U3995_000814"
     CDS             complement(29566..30075)
                     /locus_tag="U3995_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796157.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(30250..32385)
                     /locus_tag="U3995_000815"
     CDS             complement(30250..32385)
                     /locus_tag="U3995_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1796158.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(32468..34123)
                     /locus_tag="U3995_000816"
     CDS             complement(32468..34123)
                     /locus_tag="U3995_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1796159.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(34125..34439)
                     /locus_tag="U3995_000817"
     CDS             complement(34125..34439)
                     /locus_tag="U3995_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1796160.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            34609..34869
                     /locus_tag="U3995_000818"
     CDS             34609..34869
                     /locus_tag="U3995_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1796161.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            34883..35197
                     /locus_tag="U3995_000819"
     CDS             34883..35197
                     /locus_tag="U3995_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1796162.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        10262 a         7356 c         6392 g        11304 t
ORIGIN      
        1 tttgttgggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
       61 ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta gtcagaaaat agcttctcat
      121 attttctagg ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga
      181 ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag
      241 atgcaacaaa acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt
      301 aaaattcata aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat
      361 atgatgaatt tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt
      421 ttagcaattt ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta
      481 attttgggtg ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt
      541 aaatatgggg atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc
      601 gtgaatgccg tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca
      661 ggtagctggt ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt
      721 tctatgctga ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc
      781 gttttattca tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc
      841 tgggggactt gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa
      901 aagttccctg ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg
      961 tcttcgatca tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag
     1021 actgaaaaag gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg
     1081 acagcaaata tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc
     1141 tttgttaaca gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat
     1201 taccctaagg cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa
     1261 caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt
     1321 tctactagct tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg
     1381 atccagtttc catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc
     1441 ctttcactga accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt
     1501 cttatttttt cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat
     1561 gaaacatttg tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca
     1621 ttcttcagca aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat
     1681 ctgccaatct ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta
     1741 gaaaacgaaa caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa
     1801 aacactgggg acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag
     1861 caaaatggaa aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag
     1921 cagcaaaaag gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc
     1981 attctttctc ttactttgat tggctggttt accttgctat gtttatttat ttatcataga
     2041 tacaagcttt tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa
     2101 acggtataat cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt
     2161 ttttattcgt tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta
     2221 tcgatcccat caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata
     2281 aaatctatac gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta
     2341 gctgcttttc ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct
     2401 cttacagtat aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt
     2461 ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt
     2521 tttttatctg tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc
     2581 tttgttttcc caatcaagat cttgcgtgtc tgataatctt ctttcttttg aaattgggag
     2641 acggtaagga cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat
     2701 ttatcataac cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt
     2761 aaaagctgaa ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc
     2821 ggacagaact tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt
     2881 tcttgtaaag caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat
     2941 tttttctctg gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt
     3001 tttctccttg tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct
     3061 ccagcaaata gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat
     3121 tttttgtttg gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta
     3181 aacagtattc aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag
     3241 ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct
     3301 tacattttaa tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact
     3361 acttcttcta ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt
     3421 tggatcacat caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct
     3481 ttgacagcaa tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc
     3541 aaaacttcga taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt
     3601 gtcacttttc cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg
     3661 tcacccatca ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct
     3721 ccagaagctg cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt
     3781 ccatttcctg tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct
     3841 tcgatatctg atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct
     3901 ttgacgatca atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct
     3961 cgatcattta cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct
     4021 tttcctccca gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg
     4081 ttagatacaa cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca
     4141 aagttgaatt ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc
     4201 acacacaaca cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc
     4261 cgagtcagca attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc
     4321 gcaaatgcag ccccttggat cactgaataa atgacatata atggcacagc agcaaacata
     4381 aacatgaact ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa
     4441 tacattgatt tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc
     4501 attcccatca agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc
     4561 cagttatgca acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa
     4621 tcagtggccc aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg
     4681 cctgataaaa tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata
     4741 tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga
     4801 atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt
     4861 acgtttggcc acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca
     4921 aacggaacaa agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta
     4981 tagtacttat tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc
     5041 atattcaaag ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta
     5101 ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag
     5161 atagccccag tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct
     5221 gctctttctt ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca
     5281 ataattgccc aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca
     5341 ggattgatca agggaattga tttccctata ctgatcatca acccagctgc cggcataact
     5401 gctaccacaa ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt
     5461 ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt
     5521 tcttttttgt aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata
     5581 atacgtgtgt tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta
     5641 tgaaatattt tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata
     5701 aaacagctca ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca
     5761 tggaaaatca gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca
     5821 agtattggaa acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac
     5881 caccatcaag gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg
     5941 tggaaaaatg gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg
     6001 atagatattt cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt
     6061 tactgggaat tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct
     6121 actaataaag tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct
     6181 tatatctatt taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa
     6241 ttggatggaa atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt
     6301 gatcgtggaa ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt
     6361 gggattgaac aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc
     6421 atccctgact atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct
     6481 caaggtgaga catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca
     6541 gaagcgcagc aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc
     6601 caagcaaaag aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc
     6661 atcgaaggag acgaagaagc acaacaaggt attcgattca atttgttcca actattctct
     6721 acttactacg gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa
     6781 tacggcggag ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta
     6841 gccaaacctg aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca
     6901 atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact
     6961 ggtgtcgaat gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg
     7021 attgcctatg caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat
     7081 gaaggactgg aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca
     7141 aaacgagcaa ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat
     7201 atcaataaca attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa
     7261 aattatttaa cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa
     7321 tggaaagata tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc
     7381 cagcatgaca cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat
     7441 cttcctctga atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag
     7501 gcagatgtac tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa
     7561 aaaaactttg atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc
     7621 cattcgattt tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga
     7681 acagctcgct tggatttgga taattacaat aacgacacag aagacggctt gcacatcact
     7741 tccatgactg gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat
     7801 gatcagttga gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc
     7861 aattatcgcg gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta
     7921 ttagaaggtt ctcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt
     7981 ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga
     8041 cggtgtcatt accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga
     8101 aatcggcatt acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga
     8161 ctcactctct ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt
     8221 tgcagaatta gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc
     8281 aaaagatgtc ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa
     8341 gattgctctt gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac
     8401 accttacttt gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga
     8461 tatcttcctc cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga
     8521 agacgcgcaa gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg
     8581 acgtgctgaa gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat
     8641 cgattacttg aaagaaaagt ggcatgacca tggataatcc agagatctcc gtcgtaccag
     8701 ttccagagac tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc
     8761 tcaattacgg tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa
     8821 atgttttatt gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag
     8881 caactgtcgg acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg
     8941 agaaaaactg cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt
     9001 gggaagtaga agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg
     9061 aattttctac ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg
     9121 aattagagat gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc
     9181 atgcctactt caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg
     9241 tctttttaga tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa
     9301 aaaaagaaaa actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat
     9361 gttatccaga tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt
     9421 ccttacaatt attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa
     9481 gtatggtatt attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga
     9541 tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc
     9601 ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc
     9661 ggttctctgc acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg
     9721 tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta
     9781 gtatttccga gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc
     9841 ctaacatgac tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac
     9901 cccctcttga ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa
     9961 tcaacgaaga agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg
    10021 aattgctgtt agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc
    10081 tagtgtattc agatagtcaa gatactgtca ttgattattt ataccaaaac cacatccctt
    10141 tcactttgat cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata
    10201 accagcttct aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc
    10261 tgtttgtgac aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa
    10321 aagcaatgat gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg
    10381 attatctgtc ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg
    10441 acgatatctt cgcccttcgc acgatgcagt tggcgcaaat gtatggctat gtcattccag
    10501 agacactctc cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc
    10561 ttacaagtat cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg
    10621 aattgatcca tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca
    10681 aaagagagac agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc
    10741 tgaccattta tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg
    10801 tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    10861 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    10921 tttaaaaagc aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca
    10981 gcctcttttt tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa
    11041 tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct
    11101 aactttcatc gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac
    11161 ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc
    11221 aaaagaacgc ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat
    11281 cagattgatt ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat
    11341 cttttctttt aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc
    11401 taatgccaat gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg
    11461 agaaagcagt tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat
    11521 gtctgctgct tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc
    11581 tgtcgatccc gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa
    11641 aataactagt ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat
    11701 catttcattg atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa
    11761 aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa
    11821 tagattaact cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt
    11881 attccctaaa aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag
    11941 taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc
    12001 gacagtcact ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga
    12061 aacaagttta aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc
    12121 aactaagaca ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt
    12181 gatgatcccg tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa
    12241 acacatgaaa gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac
    12301 tcctaggagt gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt
    12361 attcatcgtc gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt
    12421 ggcaggacct cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt
    12481 ttcttctaaa cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat
    12541 caatggaata acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt
    12601 cgctgtacct aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac
    12661 tacccaagct aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg
    12721 tttcttacgc attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac
    12781 agttgtagca tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag
    12841 caaagcaaat aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta
    12901 tttgttttct ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat
    12961 gattgttttt gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac
    13021 catgaagctg aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa
    13081 gataggctgc gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag
    13141 catcgcattg atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt
    13201 ataatttttt gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac
    13261 aaaaataaat gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa
    13321 agcttttgac catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc
    13381 tgccaaaaca cctaaaacga acatgatgac tactgggata tataccccta gccatacacc
    13441 gatgttcccg ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta
    13501 gataactaaa atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa
    13561 tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg
    13621 cacccataat agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca
    13681 taactgagga ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat
    13741 cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc
    13801 ggctaacgta ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt
    13861 cccaagaccc attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt
    13921 ggataaaata acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata
    13981 tttgagggga ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca
    14041 aagggatcga agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca
    14101 gttagtattc aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat
    14161 aattggtctg cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca
    14221 tattcttcaa agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca
    14281 gttacttttc cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt
    14341 aatgggctgt tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta
    14401 ccgtaaatgc ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc
    14461 attgcaacta agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct
    14521 ggataagtta atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc
    14581 aagaagttgt agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct
    14641 gtgacattca aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct
    14701 tttgaacctt ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat
    14761 acatatgtcg caaagtaaga gataacgtct ctcatgcgga tatctttgat gacgatacca
    14821 ccggcagaat atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct
    14881 tcgattgctt tatatgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag
    14941 acattgtatt tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg
    15001 tctaagaaaa tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa
    15061 ataccgtctt tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct
    15121 ccttcttctg ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct
    15181 agaccacgaa cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc
    15241 actggaatca cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag
    15301 tgtttagttt gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct
    15361 gagtgagctt tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct
    15421 tctgttgaca agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact
    15481 tctttcattg caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt
    15541 gctaaagaac catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca
    15601 aattccattc ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg
    15661 ttattaccat tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca
    15721 gagttcatat gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc
    15781 cgcgctgaaa tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca
    15841 ctgctttttt cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag
    15901 cctaaatgtt catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct
    15961 ttgtccccaa tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg
    16021 aaaccctcct aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat
    16081 tctgtaaact aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga
    16141 attgttcaca cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct
    16201 cgcaaagatt gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa
    16261 tgttgtaata gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt
    16321 tggccaaaaa gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat
    16381 cggatggatt tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc
    16441 cgtttgaaac atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca
    16501 gttccacgat attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt
    16561 gcgcgatttc ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt
    16621 tttgcgcttc tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac
    16681 ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt
    16741 cttcttgaga caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat
    16801 tcaaccagaa ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac
    16861 cgcctgcagt ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac
    16921 cgaacgcttc tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat
    16981 ttccccattg gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca
    17041 aaaagtcgat agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca
    17101 aacggctggc aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt
    17161 ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga
    17221 tatttgcatc tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt
    17281 gcacttgttc catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg
    17341 ttcctgttcc gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca
    17401 tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc
    17461 aataaagtga aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct
    17521 gattaatggc gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt
    17581 agttttttta tgaaagcagg aacctgctag catatgtaat taattatttc ttcaggatag
    17641 gataggaaat aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca
    17701 cccaatttgt atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc
    17761 aatcactgcc aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg
    17821 accaccagct ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt
    17881 attattaata tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat
    17941 tttttttaaa ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt
    18001 tcaattattg ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc
    18061 actttacttt gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat
    18121 gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc
    18181 tgctttaact atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa
    18241 aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga
    18301 taagcaacaa ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc
    18361 gattgcgtta ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca
    18421 aacactaaaa aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca
    18481 tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca
    18541 aggatacaaa gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta
    18601 tcaaaatgat tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa
    18661 tctttccttt aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga
    18721 tgatgtcagc tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc
    18781 aaataacaat gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt
    18841 tgacatggtt cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg
    18901 cgcaaaaggt accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa
    18961 atctcaatat ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc
    19021 ggaacagttg aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat
    19081 gactcaagct tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc
    19141 acctatccta ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg
    19201 gcagccttat tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat
    19261 tttctgtaat gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg
    19321 aaatgctcgg atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc
    19381 tattcttgat ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc
    19441 ttgattttac gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg
    19501 cttttttatg ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc
    19561 tgctgtatat ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc
    19621 ttgaaaatag aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg
    19681 ccctcgatgg aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc
    19741 gtcagaaaat tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg
    19801 tttgttcact cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc
    19861 aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc
    19921 aaaaaatact tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt
    19981 acttacctga gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt
    20041 gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc
    20101 aaaaccgttc atttgtagag cctcccgtaa ttttaaactt ttataatata tatagttcaa
    20161 atttattctg aggtttatta taactatcta ttgagaaagt atcaagctag caattccttg
    20221 tttatgaatt cattgacaga aatttataaa aagaatatta tgtactaaaa taataaaaaa
    20281 agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat
    20341 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
    20401 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
    20461 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
    20521 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
    20581 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
    20641 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
    20701 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
    20761 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
    20821 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
    20881 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
    20941 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg
    21001 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
    21061 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
    21121 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
    21181 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
    21241 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
    21301 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
    21361 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
    21421 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
    21481 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
    21541 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
    21601 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
    21661 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
    21721 tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag
    21781 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
    21841 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
    21901 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
    21961 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
    22021 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
    22081 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
    22141 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
    22201 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
    22261 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtagtctt
    22321 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
    22381 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
    22441 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
    22501 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
    22561 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
    22621 attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc
    22681 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
    22741 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
    22801 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
    22861 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
    22921 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
    22981 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
    23041 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
    23101 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
    23161 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
    23221 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
    23281 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
    23341 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
    23401 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
    23461 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
    23521 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
    23581 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
    23641 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
    23701 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
    23761 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa
    23821 tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt
    23881 cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt
    23941 aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta
    24001 cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg
    24061 gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg
    24121 taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta
    24181 tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt
    24241 tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg
    24301 cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt
    24361 ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct
    24421 ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga
    24481 tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat
    24541 tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc
    24601 gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa
    24661 acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat
    24721 ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg
    24781 cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt
    24841 ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca
    24901 gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg
    24961 ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt
    25021 ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga
    25081 ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat
    25141 cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc
    25201 catcgccaat cggggtatct agatcactga gatattgttt gttctgattg attgcctctg
    25261 caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt
    25321 atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt
    25381 gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa
    25441 aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat
    25501 cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac
    25561 ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca
    25621 gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac
    25681 catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg
    25741 ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt
    25801 tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga
    25861 cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat
    25921 catctacgat cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat
    25981 cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct tttccactat
    26041 cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa
    26101 gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc
    26161 ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg
    26221 aagattctac acgatgaaca agttctggat agctttttac taatccttcc agcatttctt
    26281 cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg
    26341 gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct
    26401 gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct
    26461 tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa
    26521 acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct
    26581 gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc
    26641 atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa
    26701 atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct
    26761 ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa tatgtgtagt
    26821 gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg attacaacaa
    26881 caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt tttggtgagg
    26941 tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat atctttggcg
    27001 gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca cttgccgaaa
    27061 tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa atggactatg
    27121 aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca gctgtctcca
    27181 cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat gtagtggcac
    27241 tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc ctctcttttt
    27301 tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc ccctgctaag
    27361 gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact tgttgatttg
    27421 gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac tgatttttct
    27481 cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc agcttttttt
    27541 gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat tgcttgactg
    27601 gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg agcaacctga
    27661 tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca
    27721 gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc
    27781 actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc tttcaatttg
    27841 ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa
    27901 tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga agatgaaatc
    27961 gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga ggcaccgttt
    28021 tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc agaagtttcc
    28081 gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca gattaacggc
    28141 ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat ttcttattat
    28201 aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa aaacggtgca
    28261 tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa cctaaaatcg
    28321 ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa
    28381 gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca tctacaatca
    28441 gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac acaaaactga
    28501 atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc ccgattaatc
    28561 cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc aatgtttgat
    28621 caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact tttttgaaat
    28681 tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga tatagcatct
    28741 tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca gataaccagt
    28801 catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct tgtctgaatc
    28861 cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg tacgcataaa
    28921 gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt gagaattcaa
    28981 ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt gcagtgaccg
    29041 cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc agtgcatctg
    29101 gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca aaggcaagtc
    29161 cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca cgaagattag
    29221 cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg
    29281 tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg attgtatatt
    29341 cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt tcgacactca
    29401 agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa caccagacac
    29461 caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa tacataatag
    29521 acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac
    29581 cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac
    29641 agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc
    29701 ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac
    29761 ttttttgtat agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact
    29821 gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa
    29881 agcgtacaag gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat
    29941 aaaaggaaga acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa
    30001 ccagtaatta ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct
    30061 tgttactttt ttcatatcct cacctcacgt ttttttgtta cgacgtagga gtttaccata
    30121 catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca
    30181 tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt
    30241 atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc
    30301 cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat
    30361 tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa
    30421 tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt
    30481 gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa
    30541 ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac
    30601 gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct
    30661 agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca
    30721 acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac
    30781 taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata
    30841 atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag
    30901 taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc
    30961 ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca
    31021 tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc
    31081 ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg
    31141 cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa
    31201 aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa
    31261 tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt
    31321 ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga
    31381 ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt
    31441 ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg
    31501 tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac
    31561 attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt
    31621 catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt
    31681 accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt
    31741 gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc
    31801 taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa
    31861 acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg
    31921 acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga
    31981 acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata
    32041 aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact
    32101 tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat
    32161 ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta
    32221 actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat
    32281 ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac
    32341 gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt
    32401 tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta
    32461 acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa
    32521 atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat
    32581 agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta
    32641 atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca
    32701 attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt
    32761 catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca
    32821 tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta
    32881 aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca
    32941 caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca
    33001 acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat
    33061 gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg
    33121 cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag
    33181 aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag
    33241 aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat
    33301 aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta
    33361 attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa
    33421 ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa
    33481 atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta
    33541 taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca
    33601 tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac
    33661 caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg
    33721 gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa
    33781 ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct
    33841 catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac
    33901 gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc
    33961 gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg
    34021 gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag
    34081 cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact
    34141 gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata accagatttc
    34201 tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc
    34261 ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt
    34321 atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa
    34381 agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt
    34441 ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac aggagcttta
    34501 ttaatatgtc aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa
    34561 taccgataag gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat
    34621 aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa
    34681 atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct
    34741 agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa
    34801 cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt
    34861 aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg
    34921 gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt
    34981 cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt
    35041 agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc
    35101 agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga
    35161 actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt
    35221 gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa
    35281 tcaaaggttg tgaaaacgct gtcctgcatc aagt
//
LOCUS       DASJPH010000018        34622 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJPH010000018 DASJPH010000000
VERSION     DASJPH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.018"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(176..1462)
                     /locus_tag="U3995_000820"
     CDS             complement(176..1462)
                     /locus_tag="U3995_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1796163.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="U3995_000821"
     CDS             complement(1455..2723)
                     /locus_tag="U3995_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1796164.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="U3995_000822"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="U3995_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1796165.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="U3995_000823"
     CDS             complement(3495..3941)
                     /locus_tag="U3995_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796166.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="U3995_000824"
     CDS             complement(4034..4279)
                     /locus_tag="U3995_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1796167.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="U3995_000825"
     CDS             complement(4394..4972)
                     /locus_tag="U3995_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1796168.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="U3995_000826"
     CDS             complement(5049..5579)
                     /locus_tag="U3995_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1796169.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="U3995_000827"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="U3995_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1796170.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="U3995_000828"
     CDS             complement(6436..6831)
                     /locus_tag="U3995_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1796171.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="U3995_000829"
     CDS             complement(6828..8240)
                     /locus_tag="U3995_000829"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1796172.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="U3995_000830"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="U3995_000830"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1796173.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /locus_tag="U3995_000831"
     CDS             complement(9095..10555)
                     /locus_tag="U3995_000831"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1796174.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="U3995_000832"
     CDS             complement(10725..11882)
                     /locus_tag="U3995_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1796175.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="U3995_000833"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="U3995_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1796176.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="U3995_000834"
     CDS             complement(13349..13876)
                     /locus_tag="U3995_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1796177.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="U3995_000835"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="U3995_000835"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1796178.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="U3995_000836"
     CDS             14909..16156
                     /locus_tag="U3995_000836"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1796179.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="U3995_000837"
     CDS             16232..16576
                     /locus_tag="U3995_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796180.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="U3995_000838"
     CDS             complement(16693..17019)
                     /locus_tag="U3995_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796181.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="U3995_000839"
     CDS             complement(17634..18836)
                     /locus_tag="U3995_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796182.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="U3995_000840"
     CDS             18953..19468
                     /locus_tag="U3995_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1796183.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="U3995_000841"
     CDS             complement(19667..20113)
                     /locus_tag="U3995_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1796184.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="U3995_000842"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="U3995_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1796185.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="U3995_000843"
     CDS             complement(21992..22621)
                     /locus_tag="U3995_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1796186.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="U3995_000844"
     CDS             complement(22605..23069)
                     /locus_tag="U3995_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1796187.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="U3995_000845"
     CDS             complement(23556..24824)
                     /locus_tag="U3995_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1796188.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="U3995_000846"
     CDS             complement(25055..25792)
                     /locus_tag="U3995_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1796189.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="U3995_000847"
     CDS             complement(25859..26428)
                     /locus_tag="U3995_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1796190.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="U3995_000848"
     CDS             26556..27428
                     /locus_tag="U3995_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1796191.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27770..28642
                     /locus_tag="U3995_000849"
     CDS             27770..28642
                     /locus_tag="U3995_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1796192.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28639..30597
                     /locus_tag="U3995_000850"
     CDS             28639..30597
                     /locus_tag="U3995_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1796193.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30929..31780)
                     /locus_tag="U3995_000851"
     CDS             complement(30929..31780)
                     /locus_tag="U3995_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1796194.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31903..32370
                     /locus_tag="U3995_000852"
     CDS             31903..32370
                     /locus_tag="U3995_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1796195.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32633..33115)
                     /locus_tag="U3995_000853"
     CDS             complement(32633..33115)
                     /locus_tag="U3995_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1796196.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33215..34078)
                     /locus_tag="U3995_000854"
     CDS             complement(33215..34078)
                     /locus_tag="U3995_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1796197.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9902 a         7283 c         6229 g        11208 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    27541 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    27601 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    27661 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    27721 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    27781 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    27841 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    27901 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    27961 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    28021 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    28081 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    28141 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    28201 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    28261 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    28321 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    28381 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    28441 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    28501 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    28561 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    28621 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    28681 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    28741 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    28801 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    28861 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    28921 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    28981 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    29041 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    29101 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    29161 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    29221 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    29281 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    29341 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    29401 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    29461 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    29521 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    29581 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    29641 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    29701 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    29761 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    29821 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    29881 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    29941 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    30001 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    30061 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    30121 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    30181 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    30241 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    30301 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    30361 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    30421 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    30481 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    30541 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    30601 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    30661 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    30721 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    30781 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    30841 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    30901 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    30961 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    31021 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    31081 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    31141 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    31201 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    31261 attcacgttt caatacccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    31321 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    31381 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    31441 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    31501 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    31561 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    31621 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    31681 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    31741 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    31801 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    31861 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    31921 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    31981 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    32041 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    32101 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    32161 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    32221 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    32281 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    32341 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    32401 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    32461 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    32521 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    32581 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    32641 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    32701 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    32761 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    32821 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    32881 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    32941 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    33001 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    33061 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    33121 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    33181 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    33241 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    33301 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    33361 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    33421 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    33481 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    33541 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    33601 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    33661 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    33721 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    33781 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    33841 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    33901 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    33961 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    34021 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    34081 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    34141 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    34201 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    34261 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    34321 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    34381 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    34441 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    34501 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    34561 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    34621 ct
//
LOCUS       DASJPH010000019        33033 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJPH010000019 DASJPH010000000
VERSION     DASJPH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.019"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..1502
                     /locus_tag="U3995_000855"
     CDS             51..1502
                     /locus_tag="U3995_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1796198.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1851..2483
                     /locus_tag="U3995_000856"
     CDS             1851..2483
                     /locus_tag="U3995_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1796199.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3071..4510
                     /locus_tag="U3995_000857"
     CDS             3071..4510
                     /locus_tag="U3995_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796200.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4805..5023
                     /locus_tag="U3995_000858"
                     /pseudo
     CDS             4805..5023
                     /locus_tag="U3995_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5272..5487
                     /locus_tag="U3995_000859"
     CDS             5272..5487
                     /locus_tag="U3995_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796201.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5704..5960)
                     /locus_tag="U3995_000860"
                     /pseudo
     CDS             complement(5704..5960)
                     /locus_tag="U3995_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6147..7058)
                     /locus_tag="U3995_000861"
     CDS             complement(6147..7058)
                     /locus_tag="U3995_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1796202.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7077..8081)
                     /locus_tag="U3995_000862"
     CDS             complement(7077..8081)
                     /locus_tag="U3995_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1796203.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8078..10201)
                     /locus_tag="U3995_000863"
     CDS             complement(8078..10201)
                     /locus_tag="U3995_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1796204.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10206..12653)
                     /locus_tag="U3995_000864"
     CDS             complement(10206..12653)
                     /locus_tag="U3995_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1796205.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12640..13029)
                     /locus_tag="U3995_000865"
     CDS             complement(12640..13029)
                     /locus_tag="U3995_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1796206.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13089..13592)
                     /locus_tag="U3995_000866"
     CDS             complement(13089..13592)
                     /locus_tag="U3995_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1796207.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13605..13829)
                     /locus_tag="U3995_000867"
     CDS             complement(13605..13829)
                     /locus_tag="U3995_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796208.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13933..15843)
                     /gene="ltrA"
                     /locus_tag="U3995_000868"
     CDS             complement(13933..15843)
                     /gene="ltrA"
                     /locus_tag="U3995_000868"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1796209.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16589..16726)
                     /locus_tag="U3995_000869"
     CDS             complement(16589..16726)
                     /locus_tag="U3995_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1796210.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16723..17907)
                     /locus_tag="U3995_000870"
     CDS             complement(16723..17907)
                     /locus_tag="U3995_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1796211.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18166..18456)
                     /locus_tag="U3995_000871"
     CDS             complement(18166..18456)
                     /locus_tag="U3995_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796212.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18446..19588)
                     /gene="dcm"
                     /locus_tag="U3995_000872"
                     /pseudo
     CDS             complement(18446..19588)
                     /gene="dcm"
                     /locus_tag="U3995_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19664..19828)
                     /locus_tag="U3995_000873"
     CDS             complement(19664..19828)
                     /locus_tag="U3995_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796213.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19821..20495)
                     /locus_tag="U3995_000874"
     CDS             complement(19821..20495)
                     /locus_tag="U3995_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1796214.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20573..21913)
                     /locus_tag="U3995_000875"
     CDS             complement(20573..21913)
                     /locus_tag="U3995_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1796215.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21996..22331)
                     /locus_tag="U3995_000876"
     CDS             complement(21996..22331)
                     /locus_tag="U3995_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1796216.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22341..22715)
                     /locus_tag="U3995_000877"
     CDS             complement(22341..22715)
                     /locus_tag="U3995_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1796217.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22728..23042)
                     /locus_tag="U3995_000878"
     CDS             complement(22728..23042)
                     /locus_tag="U3995_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1796218.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23155..26382)
                     /gene="fss3"
                     /locus_tag="U3995_000879"
     CDS             complement(23155..26382)
                     /gene="fss3"
                     /locus_tag="U3995_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1796219.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27170..27493
                     /locus_tag="U3995_000880"
     CDS             27170..27493
                     /locus_tag="U3995_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796220.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27657..28574
                     /locus_tag="U3995_000881"
     CDS             27657..28574
                     /locus_tag="U3995_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796221.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28567..29349
                     /locus_tag="U3995_000882"
     CDS             28567..29349
                     /locus_tag="U3995_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796222.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29350..30030
                     /locus_tag="U3995_000883"
     CDS             29350..30030
                     /locus_tag="U3995_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796223.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30023..30919
                     /locus_tag="U3995_000884"
     CDS             30023..30919
                     /locus_tag="U3995_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1796224.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            31131..>33033
                     /locus_tag="U3995_000885"
     CDS             31131..>33033
                     /locus_tag="U3995_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN1796225.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVY"
BASE COUNT         9900 a         6577 c         5942 g        10614 t
ORIGIN      
        1 taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat
       61 ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca acacctttcc
      121 atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg
      181 aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg
      241 aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga
      301 cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg
      361 agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa
      421 cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat
      481 cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc
      541 aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc
      601 attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa
      661 aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat
      721 ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag
      781 gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc
      841 tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt
      901 atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa
      961 aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag
     1021 atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg
     1081 ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt
     1141 attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg
     1201 cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt
     1261 ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat
     1321 atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa
     1381 ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca
     1441 aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact
     1501 aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat
     1561 caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt
     1621 ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc
     1681 actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta
     1741 ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt
     1801 aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa
     1861 gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt
     1921 gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta tcaggtagaa
     1981 ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata
     2041 aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg
     2101 tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa
     2161 aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa
     2221 aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat
     2281 gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg
     2341 gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga
     2401 cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag
     2461 tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt
     2521 taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat
     2581 tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg ataataatta
     2641 acagtgatat ataaacatct ataatatatt attcatacta ttgatattta gtataatcct
     2701 atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa
     2761 caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag
     2821 atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa
     2881 agtaaagcga attatcacta gttactatag agtaagttca aataaagcat ctccaactta
     2941 catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga
     3001 gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg
     3061 aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat
     3121 tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat
     3181 tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt
     3241 gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag
     3301 ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt
     3361 gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg
     3421 tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga
     3481 acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc
     3541 aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt
     3601 accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat
     3661 gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt
     3721 ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact
     3781 gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc
     3841 tccattgatt tctttcggta tttttaagaa caagattttt accatgagct tgattacagc
     3901 cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa
     3961 tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat
     4021 ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt
     4081 attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga
     4141 ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt
     4201 ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc
     4261 aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac
     4321 gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc
     4381 aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt
     4441 gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa
     4501 aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga
     4561 tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc
     4621 ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg
     4681 ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct actggttatg
     4741 aatacggagc caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt
     4801 tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg
     4861 ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc
     4921 tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg
     4981 ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct
     5041 tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg
     5101 tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg
     5161 ttccttttct aattataata agatgaccac tacagtaacc tactgaaata tataaacagc
     5221 cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct
     5281 ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata
     5341 tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca
     5401 gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga
     5461 aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg
     5521 aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag
     5581 cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg
     5641 cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc
     5701 tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt
     5761 gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga
     5821 tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg
     5881 acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga
     5941 ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg
     6001 gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc
     6061 atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact
     6121 tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt
     6181 ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat
     6241 tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac
     6301 tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc
     6361 aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt
     6421 tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt
     6481 gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca
     6541 tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta
     6601 atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga
     6661 atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt
     6721 aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct
     6781 tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg
     6841 ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt atgttgatca
     6901 atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg
     6961 gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg
     7021 ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat
     7081 ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac
     7141 cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc
     7201 acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct
     7261 tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg
     7321 tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt
     7381 gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg
     7441 actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac
     7501 atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa
     7561 tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc
     7621 ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata
     7681 atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga
     7741 cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca
     7801 gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga
     7861 attggaatat actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt
     7921 ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact accatttccc
     7981 tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc
     8041 agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg
     8101 attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg
     8161 acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg
     8221 aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa
     8281 tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta
     8341 ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt
     8401 tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg
     8461 ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc
     8521 ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt
     8581 tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca
     8641 aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata
     8701 gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa
     8761 atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag
     8821 ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg
     8881 gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac
     8941 tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa
     9001 ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag
     9061 tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg
     9121 ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta attggcagaa
     9181 acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa
     9241 caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca
     9301 tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt
     9361 cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt
     9421 cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga
     9481 cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg
     9541 aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa
     9601 agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa
     9661 aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc
     9721 cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa
     9781 atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa
     9841 tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg
     9901 tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat
     9961 acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat
    10021 ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt
    10081 caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa
    10141 ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca
    10201 tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct
    10261 tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat
    10321 tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca
    10381 aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac
    10441 ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa
    10501 accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc
    10561 gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta
    10621 ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat
    10681 tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact
    10741 tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa
    10801 agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca
    10861 actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt
    10921 tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa
    10981 attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc
    11041 ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata
    11101 ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc cccttttgga
    11161 tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag attgttacta
    11221 aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt ggtaactgaa
    11281 tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc cgtatctacg
    11341 ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc aaaaccaagt
    11401 ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg cttactagaa
    11461 ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag ttttacattg
    11521 gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc tactgattcg
    11581 gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg atccaaggta
    11641 gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt ttcattgtca
    11701 gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg aacggtagca
    11761 agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg gaatgtaaat
    11821 tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc cacaattgta
    11881 ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca taagtaccgg
    11941 ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt caccaaagtt
    12001 tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg tgtacccttt
    12061 tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg tctgacttta
    12121 aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg gcgaatttct
    12181 tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata gacaaattct
    12241 tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc atctgtcgca
    12301 atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat catggatact
    12361 aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg cccggtaatt
    12421 tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat ttgtaaggca
    12481 tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg gacttgatat
    12541 ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta ggcaaaacat
    12601 tctccctctt gattaaagac aagattattt tcaatatact taatcgggta catagttcac
    12661 actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga ctttctttcc
    12721 tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa atcccaatgg
    12781 tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg gtatacctaa
    12841 gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa atacgatgac
    12901 aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa aaggcagttg
    12961 aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt agctttttat
    13021 tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac tacccttcaa
    13081 taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata attgcctcca
    13141 atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg ttctggtact
    13201 tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat atcatcgcaa
    13261 tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga attgaagaaa
    13321 gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc ctccgctaag
    13381 ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa gggaagttca
    13441 taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc tttcacttca
    13501 tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt atatttcccg
    13561 aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc accgacaatt
    13621 cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac caatccgacc
    13681 gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt aaatcctaaa
    13741 aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt taaaaaccat
    13801 tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc cccccattca
    13861 cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt gaaaagtatt
    13921 tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc tatatttatt
    13981 tagtcttatg agctggactt tatttagctt caatagagct atatattttt gtattgtttc
    14041 ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa gatttttata
    14101 atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt cattagcttg
    14161 ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata gcgaaattcg
    14221 attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact ggtacatcag
    14281 taatttatca acacttagat tggtgtggat aaattttctt ccctctagag tgtagcaatt
    14341 cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag ggaaaatagg
    14401 ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg ttgcctcctt
    14461 tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac ctaatctgtt
    14521 agtgaagata gccattacag ctctattcag tttcatacaa tccagattta cattcgttgc
    14581 aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg tttcttctaa
    14641 ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca gcttgtcttt
    14701 ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct ttcttctcgg
    14761 tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat ttacaattct
    14821 tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag tgattgctat
    14881 tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat ctgcataacg
    14941 aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt tgcttttaca
    15001 tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat tttcctgcca
    15061 ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta gtggggatat
    15121 tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag tcattttgat
    15181 tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta ttcccatagc
    15241 ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct taatatcaaa
    15301 ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt ttattgcatt
    15361 ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag cttcgcagat
    15421 tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac aaggaatacc
    15481 tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg gcttaggtcg
    15541 atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg tttctgctgg
    15601 caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac tgcctgcatt
    15661 tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa tatccataag
    15721 atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg tgttttgcat
    15781 ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt tatttttagg
    15841 cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac tcgtaatcct
    15901 tattgaaaca acataatact tttctaatat atacacgact tgagggcatt cctccattct
    15961 cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa ctgcttattg
    16021 ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc cgataatctt
    16081 atctatacat atatatcctt aggtcactag ctataagcct gctggcttga taccgcctgt
    16141 aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct acgatttctc
    16201 gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt ttcgtcagtt
    16261 ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg gatacgctac
    16321 ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg gtatctttca
    16381 gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg tcggaggctc
    16441 agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc agttctccta
    16501 ataaccaaac tttttcgttc ttttattagt gccttagctt ttcactaagg aacgagtcgc
    16561 acataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt gctcgatccg
    16621 ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg ccaccaaata
    16681 ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa tcaaattttc
    16741 aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc gtgcattctg
    16801 taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag ccattttcca
    16861 cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat acggttttgg
    16921 ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc attgttcatt
    16981 caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca agtaacgatt
    17041 gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt gaatcgcatg
    17101 aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag tttggtctaa
    17161 tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga aataaacctc
    17221 actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat cagcctttaa
    17281 aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt cttccttctg
    17341 acattttttc gttagctcag gtatatccaa tagccccact cgatcattga ttgccaagtc
    17401 caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt accagctacg
    17461 cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta attctaaaag
    17521 cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga catactgttc
    17581 ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca aatggagaat
    17641 ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat agtcaaacag
    17701 taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt tcctcatggt
    17761 ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc gactaagtcc
    17821 acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt cttttaatag
    17881 ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt gttaactttt
    17941 tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa gcattcttaa
    18001 tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg ggtgagacat
    18061 catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt ctgcttcgcg
    18121 aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact catctgtaat
    18181 accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat ctggttcttt
    18241 tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa ttcgagcatc
    18301 cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct tgcgaatatc
    18361 aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct ttccaatcac
    18421 ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct tttaatcttc
    18481 tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta tacaactgac
    18541 tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc tgaagtcgcc
    18601 aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc gcttgttggt
    18661 ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga cttgtggaat
    18721 taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct gtttcatttt
    18781 ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg gttccagtaa
    18841 cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct acagagattc
    18901 catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt ggcgctacgc
    18961 catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata tttaccaaat
    19021 ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact tgttcggtac
    19081 atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga atgtaggcct
    19141 ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt cggaggatcg
    19201 tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca aggaataaaa
    19261 gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta cctcgagtat
    19321 cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat ccgccgcaaa
    19381 ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg atgtcatgca
    19441 tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca aatttatcaa
    19501 tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga aagccaccaa
    19561 taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa gaaaaaagcc
    19621 aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt ttatttaatc
    19681 caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc accgttttga
    19741 actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat ttctttacaa
    19801 ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt cctactatac
    19861 cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga aatattattc
    19921 gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt tacttttgca
    19981 atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac atgacgtccc
    20041 tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg ataaacatag
    20101 aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc agcacttaat
    20161 attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata tctaaatgcc
    20221 tgctctacca accaagaaga tgctttttga aagagactag agttttttcc attataatca
    20281 ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata attctcagct
    20341 aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa aactgcgtct
    20401 cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc cttatttgct
    20461 tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt tcttcctact
    20521 atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc actcaatcat
    20581 tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt acaagtggcg
    20641 tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct ttgatgtgct
    20701 tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt tcactcatac
    20761 gacccaaagc cacgcgaaag ttgaattgat cgcggattcc gtctcctaaa tatttcgcat
    20821 ccggcctttg gcaagccaaa atcagaaaga aaccagattg acgacccaac atcacgattt
    20881 gtttgagctt attcaaaatc accgcacttt ctttcgtcgt taacatctcc atataagcca
    20941 cgtattcatc aaagattaaa aagttgggtg gaagtccaag ataggcataa ttctctcctg
    21001 ttttgtagtt agacatttct ttcattgcct tgctacgagc catcatacgt tcatagaaat
    21061 cttccacaca agcagaaatt tcttcctttt gtgaataaac atgaggcatc accgtaccta
    21121 aatcagctaa atctgcattt ttgggatcga ggataaacaa ttctgcatct gacttcaata
    21181 gtgcttcgat aatggtgagg aggaaatagg tcttccctcc acccgtgcca ccagcgatta
    21241 acatatgggg taaagaatca taagcccata cttgattttt catcaatcgc agagtcccat
    21301 tttctgccac tacttcgtct atcccaattc gattggcgat catatcatac agtaaagtat
    21361 actccacata ggagtctttg agttcctttt ctactaactc acaatacagt ccgctttcta
    21421 acttcttttc caattttaaa agctgatctt ggtatttccc aagtgaaatt tgaacgcgaa
    21481 tagacagtaa tccatctttc attcgataac agattttggg gaagtaactg atagtctctc
    21541 tggttcggct actattcaaa tctttgaaaa agccttcact ttgagtttgt ttcacttcat
    21601 accaatggtt ctctagaatc atccgagcta gcttttgtcg atgccacaag cttcgccaat
    21661 gatcaagaaa aaagtgaata taaagcaata tcatagctcc tgaaaaagcg agacttccaa
    21721 gcagacatac taaatctttt atagaccaaa cgatttcaat agtagaaagt gatagcttag
    21781 aaatatcaaa ttgtctaaac tcttgaaaat aaaagacgct catccaaccg atgaacagag
    21841 cgagtagcca gccaagaaca aagtgataaa ccaagtgctg atctcctgcg cgtatgcgat
    21901 gtcctttgta cattttgatg gtttccacct ccaatcgaaa aagcccaata ggatttcatt
    21961 aggcaatttc aaaaagttat gctagtcatt tactgctacg aggaggggag ttgtttcgtt
    22021 tcaaccaatt gatttaaaaa gccttcaata gcatccggca tctccacacc aatttcttcc
    22081 aaaatggatt ctgcctgccg acataagttt tcatttactt cgacaactat aaagaaagac
    22141 tcttcttctc ctaaccagtc tagtacatca gagtcttctt cactgtcgct attttctcca
    22201 agtaaacctt gatgtataat cagaaacatt ttttcatcag aggttaatgc tttaacgagt
    22261 tgttcctcag tcagctcgcc ttttccatat tgatcgagac aatctaaaat atgttcaaca
    22321 ttaatttcca taatcttcac ctatttcttt ctttcatttt ggttcgtagg atttccttga
    22381 ttcggctgcc gcttcaagac taaatcatcc gccttaatga accaattaac ggtagttcct
    22441 tctcgaaaaa cataattggc aacggtatcc acgattggat tgaccaactc tacctctgca
    22501 tcataagcaa actcgcgtaa gggtacatta gctgggatac taacttgaat cattcgccct
    22561 tgtgcagagg attttaaatt ataggttcgt tcttttacct cattaccaat tgttccatcc
    22621 tcattttgct tccgtacttc tcggcgtaat gcggaaaaac gcaacttccc aaatgttaaa
    22681 cctgaatcta cgacaatacc ttctgttaat ctcatcttct tttcctccta tgctttcact
    22741 aaatcatcaa catacaacac gtaatcagta aacccacgat tctcaatctc atagcctttg
    22801 gcaaccaaat ggggattcac ggcttttagc ggttggtctt gatcaaaatc cttttcaccg
    22861 gcttccgcag ctactaccac ctcaatatca tcggcccgtt gcttactgga atacaaatga
    22921 taactgcgac caatcaccgt agtattccgt ccatatcctt ctgttataat ttctccttca
    22981 ccggcataac ttaactttcc aaaggtttca gccatatctg gcacgacaaa tttcaattcc
    23041 atcttctttt cctcttttca ttgttagttt tggatacaaa aatagcgcga ctactttttt
    23101 aggaggcggt cgcgcacgtt ggttcttagg atttacttgt atcatctcat caccttagtt
    23161 ccggcgattc cagaaataat agcccgccgt cgcaaaaatc aaagtaaaac caatgaacag
    23221 taaaacattg gaatttttct ccccggtttg agggaaggtc ttcactgtgg gactagattc
    23281 ttgagagcca cttggaacag ttggtgtttc cggttgtttc ggcgtactta gtatggttgg
    23341 aacttctttt ggtgttaagg tttgagattt ttctttcaaa tcttcattgt gttttccatt
    23401 gatattccca tctttatcgt aattgatttc cgcaaaagta aacttggttc cttctggata
    23461 ttttccggta tctacttttt tcgcaagcac agttttggtg aattctttat catttacttc
    23521 ataatcaatc ttgccagatt tccaaatttc tttgtttgta ccatccggta ataaagcata
    23581 cagaattgtt tcgaaagctt cttttgagcc atccagtaca tcatgagtaa tcgacacatc
    23641 atcaaacata tccaccacat caccatgagt aaaggtctga gaaccgtttt ctaggtgggc
    23701 ctttgtttgg atagaaacat ggtgttccac tgtacaactg acagtttggg cttggttatt
    23761 caagctaaca tccttcgcta ctggcttatc gcctgcttca aaagcttcct tattttcata
    23821 cacatagttg aataagacaa tagttttatt taaaacttgt tcggcggtta atttatgctg
    23881 aattttccaa gttcctgttt tctctttgtt cgccaacgtt gcgcttgttt ctgcaatcac
    23941 cactggtttg gctttttcat ctttttcttg ggcagctttt gtcgcatcca catcaatggc
    24001 ttgcgctacc acataccagt cttctttgat ttcatgtaga ttgtaactca atttatcaac
    24061 tagctcggta gaatccagag aagttaattc tttgtttccg tctttccaag tcgcaagaga
    24121 agttaaacta tcttcttcct caggcaaatc atagagcatt aaacggttca aactaacaga
    24181 aaatgctttg ttcgtcagct tctcgtaagg aacggtcacc gttttaattg gttgtttttg
    24241 gtcttgttcg gtaatggtaa agacatactc actcttcaca aagtcttctt tgttttcctt
    24301 aaaagtagaa cggatttcta atggcgtaat cttttgaaaa ccttctgggg cttctacttc
    24361 ttcaagtaaa taatccccat aaggaagatt ttcaaacttg ccataaccat caaaccctaa
    24421 ttgctcgtta taagctgtgg tcgcttcatc ttcggcacct gtgatttcat tggtcccttc
    24481 caatggcgac actttaaagg tcagatcatt gaagccagtt tcggtagtgc ccgcagctga
    24541 tccagcaaat ttaaagaaat caaagccaaa gcgaatcact tgttcttttg ccgtaacatt
    24601 tcgggtaatc acggcatttt tttcgctatc atccaccttt ttgatggata caggatactt
    24661 tgtttcatca aggctatatc cttcgggcgc ttttgtttct tgccaataat actcgttgat
    24721 agctaaatgt ttaacggcaa cttggttctt ttcgtctaaa gtcaaagtga ccgtttcatc
    24781 agaacctttc gttcccttcg ctaactctgg tttaaaagcc tcactccatt taacagcttt
    24841 tccatcctta gcagtaaaca aggtgtattc agcaccctca aagactgcct taccttgtgc
    24901 tttatcccca gtatctttgt cttctttcgt caaagtggtt tcgccagtga cttcttgatt
    24961 ttgccctttt acgttactgg taacaggggc tacggtttga ttggcatact tcaattcgac
    25021 ttttacaggt ttgaacgtat tcacgaaccc atgactagct ttagtttcgg tcacataata
    25081 ggttcccaat tccaacgcat tagcaatctc ttttggtgtt tccgcatgac cgttttcatc
    25141 cgtggtcatt tcttgaacga tttcaccggt tggactgtct ttgcgaatgg caaacgtatt
    25201 accagctaag gaataattgt cattccaaag atcactccct gtttcaactc cgcttttatc
    25261 cagaatgatt tgcccttttt cccgtgcgtt ttttgaagtc acgtaaatgg tttcgcctgc
    25321 tttaatggtg gtagtcatgg gggtggtatc aatcgtataa ggcgccggta ctgatttttc
    25381 ggtaatggtt acttttgtac catgaggaat ctcatccaat gtagcaatgc cttctttgtc
    25441 agtagtcacg tcttttgcag gtaaagtttt tccaaagtct aaatggaaaa ccgttcctgg
    25501 tacaacatca cccgattctt tatcgacttt tttgatcttt aaagaaccct ccgtttccac
    25561 atcaattttg attgtgtagg tattaggttt atcaatcgcc ccggccatta aagtctgttg
    25621 gccagccttt ttataggcga caggtgttcc ggtaccagca gatttcttaa gcgtcagcac
    25681 accactttct tttgaattgg catttggggt gattatcaac tgattaccat tcacacgata
    25741 atcaatgttt gcggcatttt ccacaacctc atcgaactct gacaaattca gattattcgt
    25801 atccgtcaca gtagtcgatt gccctaatac tgttttagcc gtactattat ggaaggttgg
    25861 tttcttctga taatcgtcaa tggcttgatt tatttttgcc tcaatggctg cgatatttac
    25921 ggcactacca tctgatcgtt ttattgagtg aagggtatac ccattaactt cttgccagat
    25981 catcttctgt gccacaatat gagtatctgc atctgtacct acttttttcc ataaaacgga
    26041 aactaatttg gctttatcgg acatatctgg taacgggttt ttctcatagc caggagtgaa
    26101 ctcattgtaa atcgggactc ctggttcaat acagaaaata tcttgtttaa agccgttgta
    26161 cactgcataa tgcggaacag tgacttctga cattggacga ccatcactaa aagttccttc
    26221 aacagcatat tgatgtgcta aatctagttc tacggttacc gtttgtggat gggtaatttc
    26281 atcagcaaaa gtaatcgtag gagcaattgc tccaacaatt gcttgaacag ccaaactcaa
    26341 aatagttaat acacataatt tctttttttg ttttttatac atgaaatgtt ctcctttcaa
    26401 attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag tctttttaaa
    26461 atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat ttaatctaaa
    26521 atacgaaata tactgccttt aaattagaac acattttaga acacaaacat ttcaaattta
    26581 atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt tttcaaactt
    26641 attcaatgat acgaagaaaa aaccaggtct taagaaagcc cgtaaagctt cacagttctc
    26701 aaaacgttaa ttgcgattat cgttcgagtt cacgagttac gagaatcgta ctgggcgaag
    26761 caatgctgag cgcagttacc ttaatcctaa cttttctggc actttccaca atttggaaag
    26821 tgcctttttt gtgtttttag ctatgttaat aggtaggatt cttggtctat aatatcattc
    26881 gatctataaa caaacgggaa aaagataaag tatcatcaaa caatattatt tatccgcttc
    26941 tgaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttaca aaaattgctt
    27001 ctcatgtttg ataaatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
    27061 tcggaagaag ggattgtgaa aagtcttttg tcataacctc ttatttggta gctaattgta
    27121 aatcccaaac ggcatgtatt atttttcata agtccgtttt taaggattaa tgttttcttt
    27181 tgctttttat atggtctggc ttcagcgaga agttccaacg catatacaag gacgacttat
    27241 gtcaacgata acaacgatta ccatggtggc cagtccactt ggattagcac tgtttggtgc
    27301 gctctttgat tggaagacaa cgcaaccacg tctgcgagac agtctaattt ttctgggagg
    27361 gagtcttgct atagttatcc ttataaccgg tctggtaaaa ttactcaagc ttgatctgaa
    27421 agaagcaaaa attttcagtg aagaggaata cagagaaacg actttggtcg aaaagaaaga
    27481 atcaggaaac tgatacaagc gtataacaaa ctgcctgata ataggaaaga cacgtattgt
    27541 ccgattgttt tttttgacat tcaattggaa aatttacact ttctttatac atttctggct
    27601 gctttcttta tacccgtcaa ctatacttca ctcatcaata agaaagaagg ataagtatgg
    27661 attttggaaa tgaaacggtc ttgaaagcaa ctggtataac caaaaaatac ggtgctgcca
    27721 aagcattaga taaagtatca atcgaaatca aacgtggaat gatctatgga ttgattgggg
    27781 aaaatggagc gggtaagtct acctttatga gaactattat gggtttgatc tcgattgatg
    27841 aaggttcgat cgaattattt ggaacgacgg atttgcaggc agcaagacga cgtatgggtc
    27901 aatcgattga aacccctgct ctttatccag aattaactgc aagagataac ctacgtatcc
    27961 aagcagcgaa cggcggtgtg tcggatagag aaatcgaaga cctgctgaaa atgatgcgat
    28021 tggaaaatac agggaaaaag aaagcaaaga atttctcttt aggtatgcgc cagcgtttag
    28081 ctattgcaaa tgcactgatc acgaatccag aatttttgat tttggatgaa ccgacgaatg
    28141 ggatggatcc agcagggatg gcagaaatgc gagaaatcat ccaacgtttg gtaaaagaac
    28201 ggggcatcac cgtcttgctt tctagccact tgctagatga attgtcacag atcgccactc
    28261 actatggtat tttgcatgaa ggacacttga tcaaagaatt atcgaaagaa gaattggcac
    28321 aagaatctcg tcaatttata aaaattgaca cttcggcgac ggaacaagca gtaacggttc
    28381 tcgactctct tggttatagg gattattttg tacaatctag ccgtgttatc cagctttttg
    28441 aaggaattga ccaagtagca gccatcaatc aagcattggt agaagcaaaa gttcctgtgg
    28501 atggtattca cctagttggg caaaaactgg aagattattt cttacaatta actggaggca
    28561 atccacatgt ttaatctatt aactgctgaa aaaataaaag tatggaagag ccgtaaaatg
    28621 tgggtctcac taggactgat ggtcgtactt ccgctttttt attcttggaa cgaatggtat
    28681 atgcatacaa aatacggcaa tgaattaaac caagcaacgg atacagtcat caacggtgca
    28741 acagggattt taatggtaga aaaaatggct gcatggattc ttctggcttt cgcagcattt
    28801 gcgtgctttt atattgggga agaatttcaa aacggtacga ttcgcaatac gttatcttta
    28861 ggacggaatc gaatgactta ttatgtgtcg aaacttctcg ttacgctttt gatcacaaca
    28921 cttggcgtgg ttttgatcac gggactagcc atgcttgctt acacacttgc tttcggattt
    28981 ggtgaagttg agggaatcaa aaactatgga aattatgttc taaaagtatt tcctgtattg
    29041 cttctgctga cattagggac attatctgtt ccagtggctt tgacattcat cactagaagt
    29101 accagtgtca gtttgcttct ttcttttctt tatatcatgg gaacggcctt cattcccggt
    29161 gttttcgcaa aaatcaaagg cttggaattt ctaacggagt ggtttacgga aacgtggctc
    29221 atgtatacgg actttgcgca acaagcaact tattcgcaag caccgaaaat gattctggtc
    29281 agtctagtaa caatcgtggt atcatcagct ctaggaatgt ttattttcca aaaatctgac
    29341 attaagtagg tggtcctgct ggaaactatt ttgattatcg aagacgatga agctgttcat
    29401 tccttactgg aggaggttct tgaacagaga tacaaacttc ttgatgctta ttcaggtacg
    29461 gaaggaaaat tattgttaag cacatatcct gtcgatttga tccttctaga tctcatgctt
    29521 ccagggcttt caggtgaagc attgttagca gaaatccgac agacatcgaa tgttccgatc
    29581 atcgttcttt cagcaaaaag cgatcaaaga gataaagttt ccttgttggc agcaggagcg
    29641 gatgactatg tgacgaaacc atttgatatc gaagaacttt tgttacgaat cggcatccaa
    29701 cttcgtcatc agacttctgt ccaagtaaaa gatctgtcgc aacgcatcta ttacaaggaa
    29761 atcttactgg acaaagagtc gagggatgta aatgtgaatg gtgtggctgt ccatttgaca
    29821 ggaagagaat tcgatctgct gaagcttttt ttagaacatc cgaaaaaagt attttcgcga
    29881 gcaaatcttt atgaaacagt ttggcaagaa ccatattttg atagtgagaa aacagtgaat
    29941 atgcatattt ccaatcttcg agggaagcta gcaaaggcac atgctgtgta tattcatacg
    30001 gtgtggggag tggggtttag atttgattaa atggctgttg cttctcgttg ttatctttat
    30061 gggaagtttt attttcttcc tgcaaaggga aatcaagaga ttaactaaga aaatcgccac
    30121 tctttctgaa cgacaagaat atggtggtcg tctttcagtc gaatttcgag aaaagaatct
    30181 aatggaccta gtagatgcac tgaatacatg gttggatgaa aatgaacaaa aaggacaagc
    30241 atttcaagaa atggaagaaa acatcagact atcgatcgtc ggcctttctc atgatctcag
    30301 aactcctttg actgctatta acggttatgt acaattactg gaacaggtag aggatacgga
    30361 gaaacgggag aagtatttaa cgatcatcaa acaatctgtt gcaagattac tcagtatgac
    30421 ggatcagttt tatgatcttg ctcgaataga gacgaagcaa aaagaaatgg atctccatac
    30481 gctttctttt tctaaagagg tggaagaagt atttttctct ttttatgaac aattttctga
    30541 gaaaaatatc gaggtagcgt tcgctgaaca ctcgataaat acaactgtat tagcagatcc
    30601 gctcatgttg ctgcgagtgc tgcaaaatat catacagaat ttattgcgtt atgcagatgg
    30661 acaagcaaag gtgacatttt caaaagagga tccattcatt cgattaacag tggccaatcc
    30721 tatcaaacca ggaacgcaaa cctctgttga aaaagtcttc cagcgttttt ataccgaaaa
    30781 taccagccgc acaaatacgg aatctagcgg aatcgggctt tacctgtcaa aaaaattggt
    30841 ggaaggaatg ggaggagaga tgaccgctag attagacggg ggcatcttct ccatctccgt
    30901 caagttgcgg agggtttaat tatacaagat tagtgagaac tagtagaaaa atgtattatc
    30961 ttatatatat ttaatagaga aaaaagcctg tgatataaat attgcaggct tttttattga
    31021 tttgaaataa tcaagaatat tagaaacttc tacattaatc aagaactttc ttctgtatga
    31081 aaatagagat aataattgta cacttaagct gtattcagga ggtgaagata atgggcatcg
    31141 aatttagcga acggcaaatg aaaatactat actttttgat tgaaaatgag cacacaactt
    31201 acaaagaatt atcagaacaa ttatatgtat ctacgaaaac aattagcaaa gatattttaa
    31261 ttttgaaaga aataatccaa aaaatgccag atgtacaaat gttggttcaa cctaaaaaag
    31321 gcgtttcgct tattggaaaa aaagaaacac ttcgaaattt attatccagt cataaaatca
    31381 atatcgcaga ctctccggag gaccgtctaa cttatgtgtt tgctaaatta ttgaatacta
    31441 atgattctct aaagattcaa aatcttgctg acgaattata cgtgagcagg tcgacggtag
    31501 aaagcacaat gaaagatgtt agaaaaagac taaacaagca tgggatgtcc ttagtttcag
    31561 atcgaaatgg cgtgcgcatc aaagctgatg aaaaaagtaa aagaaggttg atgtcagaag
    31621 ttgtgaacta ttattggggt ggctttacag catctaacag taaaacagat ctcttagact
    31681 tgaatatcaa tttcattggt gaagcgaata atttcttaaa taaggacata ttgaaaaaag
    31741 tagccgaatt gcttaatcga tacatgaata agacacaatt gaaatttaca gattatgaat
    31801 ttcaaacttt agctatccac cttgctattg cgatagaaag gattgaaaca agcaatttct
    31861 tatcacgtag taataattct gaagaagaaa tctcaaagaa tacacttatt ttaatcaaaa
    31921 tgattgaaaa agcattttca attattgtgc caatagatga acaagcttat gttgacattc
    31981 acattacttc gattgaaaaa aatgctatga atcacgactt agatcaaacg aagctttcct
    32041 atttagatat gggtgttgag ctacaagagt tagtaagaat taaattaaat gaattttatc
    32101 ctgatgaaga attgatcaat aatctagtat tacatctcaa tgcagcagtt aaaagactca
    32161 aattgggagt aaatatctat aacccttata cggacaaaat caaatattct tttaaaaggt
    32221 catttatgat aagtgtagat ttattagagg aaatcgaaga gagattctgt atacatttta
    32281 atgaagatga aatcgcttac gtaacactcc atattcaatc acttttggat cgttacaaac
    32341 ctgacaaaac gaaagtgata ttagtttgta gtagcggata tggaacatca aaattattag
    32401 agcaaagaat aaataacggt tttgcagata tggttgagat agaagatgta ctaagcatca
    32461 atgaactaca agatagaaaa gtcactgatg aacttgttat aagcacgatt ccgattgaaa
    32521 atacaaattt tcctgttatt gttgttagtc ctttgatgtt aggggcagat gtcagaaaag
    32581 taaaacagta tctacagtta aatgaacaaa cagaagatgc ttttctaaat ttattagata
    32641 aacgattttg cttttttacc caccaaataa aggataatca agtgaacgtt ttaaatataa
    32701 ttaccaaaaa attggtaaaa gaaaattacg cgaaagaagg gatactagag ggagcaatca
    32761 aaagagaaaa actatcttca acagcgatga atgattttgc tatgccgcat atcgaagtag
    32821 attttataaa aaagccagta atctgtgtct acgttaatcc agaaggaatt gattgggatg
    32881 ggacacttgt tcattctgtt ttcttttttg cacttaatga aaaagtaaaa cctgaattga
    32941 ataaaattta tgaaacattc aatgaaatcg cttcgaatca tgagttatta gcaaatttaa
    33001 aaacgagttc gtcgtttgaa aaattagttt ata
//
LOCUS       DASJPH010000020        32788 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJPH010000020 DASJPH010000000
VERSION     DASJPH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.020"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..188)
                     /locus_tag="U3995_000886"
     CDS             complement(<1..188)
                     /locus_tag="U3995_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796226.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIV"
     gene            375..842
                     /locus_tag="U3995_000887"
     CDS             375..842
                     /locus_tag="U3995_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796227.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            839..1081
                     /locus_tag="U3995_000888"
     CDS             839..1081
                     /locus_tag="U3995_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN1796228.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1099..1401
                     /locus_tag="U3995_000889"
     CDS             1099..1401
                     /locus_tag="U3995_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796229.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1473..3527
                     /locus_tag="U3995_000890"
     CDS             1473..3527
                     /locus_tag="U3995_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796230.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            3527..6139
                     /locus_tag="U3995_000891"
     CDS             3527..6139
                     /locus_tag="U3995_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN1796231.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            6136..8667
                     /locus_tag="U3995_000892"
     CDS             6136..8667
                     /locus_tag="U3995_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796232.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            8719..9051
                     /locus_tag="U3995_000893"
     CDS             8719..9051
                     /locus_tag="U3995_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796233.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            9052..9645
                     /locus_tag="U3995_000894"
     CDS             9052..9645
                     /locus_tag="U3995_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796234.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9808..11502
                     /locus_tag="U3995_000895"
     CDS             9808..11502
                     /locus_tag="U3995_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN1796235.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            12190..14067
                     /gene="ltrA"
                     /locus_tag="U3995_000896"
     CDS             12190..14067
                     /gene="ltrA"
                     /locus_tag="U3995_000896"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1796236.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            14198..14575
                     /locus_tag="U3995_000897"
                     /pseudo
     CDS             14198..14575
                     /locus_tag="U3995_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            14575..15780
                     /locus_tag="U3995_000898"
     CDS             14575..15780
                     /locus_tag="U3995_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1796237.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            15796..16440
                     /locus_tag="U3995_000899"
     CDS             15796..16440
                     /locus_tag="U3995_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796238.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            16451..17257
                     /locus_tag="U3995_000900"
     CDS             16451..17257
                     /locus_tag="U3995_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796239.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            17279..17509
                     /locus_tag="U3995_000901"
     CDS             17279..17509
                     /locus_tag="U3995_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796240.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            18055..18330
                     /locus_tag="U3995_000902"
     CDS             18055..18330
                     /locus_tag="U3995_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796241.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            18372..18794
                     /locus_tag="U3995_000903"
     CDS             18372..18794
                     /locus_tag="U3995_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796242.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            18813..20405
                     /locus_tag="U3995_000904"
     CDS             18813..20405
                     /locus_tag="U3995_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796243.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            20417..20752
                     /locus_tag="U3995_000905"
     CDS             20417..20752
                     /locus_tag="U3995_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796244.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            20899..21093
                     /locus_tag="U3995_000906"
     CDS             20899..21093
                     /locus_tag="U3995_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796245.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            21125..23497
                     /locus_tag="U3995_000907"
     CDS             21125..23497
                     /locus_tag="U3995_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796246.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            23558..25018
                     /locus_tag="U3995_000908"
     CDS             23558..25018
                     /locus_tag="U3995_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1796247.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            25029..27233
                     /gene="topB"
                     /locus_tag="U3995_000909"
     CDS             25029..27233
                     /gene="topB"
                     /locus_tag="U3995_000909"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1796248.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            27249..27398
                     /locus_tag="U3995_000910"
     CDS             27249..27398
                     /locus_tag="U3995_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796249.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            27472..28065
                     /locus_tag="U3995_000911"
     CDS             27472..28065
                     /locus_tag="U3995_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1796250.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            28081..28980
                     /locus_tag="U3995_000912"
     CDS             28081..28980
                     /locus_tag="U3995_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1796251.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            28983..29468
                     /gene="ssb"
                     /locus_tag="U3995_000913"
     CDS             28983..29468
                     /gene="ssb"
                     /locus_tag="U3995_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1796252.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            29834..30094
                     /locus_tag="U3995_000914"
     CDS             29834..30094
                     /locus_tag="U3995_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796253.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            30535..30741
                     /locus_tag="U3995_000915"
     CDS             30535..30741
                     /locus_tag="U3995_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1796254.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            30741..30992
                     /locus_tag="U3995_000916"
     CDS             30741..30992
                     /locus_tag="U3995_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796255.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            31007..31444
                     /locus_tag="U3995_000917"
     CDS             31007..31444
                     /locus_tag="U3995_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796256.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            31437..32144
                     /locus_tag="U3995_000918"
     CDS             31437..32144
                     /locus_tag="U3995_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796257.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            32525..32704
                     /locus_tag="U3995_000919"
     CDS             32525..32704
                     /locus_tag="U3995_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796258.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT        12268 a         5222 c         6766 g         8532 t
ORIGIN      
        1 tcaacgattt cttcagccaa tttatttaat ttcatagcat ttatgaatct catgttgtct
       61 atactagagc gtttgttggc aaggtcactt actgtagtta agggtacgcc agattctatg
      121 gatatagaac gaactgattt accactatca aacagcaacc attcgatttt ctttttatca
      181 gcgtacattt caatgctcct tcttgttttt tctttattat agctcttttt tgtaatgaat
      241 cacaatatca agagaaaaat tttctataga aaggtggatt tttatgagta ttcgttctcc
      301 gtgtatccaa atgaggtttg ttgctacttt cgttatttga tatactaaaa ataaacaacg
      361 aaagaaggaa taaaatgaag aaaaagcaac tatggtttgt ctctatgttt gcaatatttt
      421 ttattatttt aagtggatgt tcaagtaata agaaatcgga agctaaagaa gataatagtt
      481 tattgcaacc aggaactgtt tggaaagaag aagtaggtgg tcttattcat aatttaaaaa
      541 ttatagatga tactacctgg gaatattctg aaagtgtatg gcatccagat cctgtacaaa
      601 taacagttaa gaggcaaaaa gactatgaag gtttagaaag atacaaaata gtagattctg
      661 caggcattag ggaatttata aacaaacctg attcattatt tattgttgtt ccctatgaga
      721 aggatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgagaag cgaacgaaag
      781 agaaattaat tcatgatgga tcagagttaa ataaatacaa attacaaaag actagtgagt
      841 gattgctagt ctttttattt atcctgaagg aggaatcaca gtggcaaaag ttaaaacacc
      901 attcgaaaaa gccgcgtcta aaatcttaga acgccgtggt gtttctgaaa tacagtggaa
      961 aaaagaagtt cttgaaaaag cacaatttga cctattacga ggaaaagata aggacttaga
     1021 agaatatatc tatcaacgtg aacgtgaaaa acttgtattc gatgaaatta ttaaacatta
     1081 aaaggagcga ataatcttat gtttgaatca attacaatga attttttaac tcaaccgaaa
     1141 gtagtagctg cagttgatat ttttagagca ttacaaactg ccgcaactag tttgaaaaat
     1201 aatctgatga tttttgcggc tgtagctgcc gttgcctcca ttgctcttgg aggtattttc
     1261 ttcttctttg ggcgaagagg agctgaaaac gggaagacac acgtaggtta tgtattgttg
     1321 ggggtaggag ttattttagc tgccgcttct attgtagttt caatgttctc aatgtttgga
     1381 caaaccccac aaggactata aaaaaaacaa gtaggaggag taaaaagtac gtggctactt
     1441 tttactcctt tttttatgaa aggagaatat tcatgttttt tacgatttca ggaaatgcaa
     1501 aattggataa agagttaata agaaaagcaa cgaagaaagt gggaaagcgt acttcctttt
     1561 taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat gtgttaggac
     1621 aagtgttaga acaagagaat caatcttctg ttaaattgac tctcacgtta catgataaag
     1681 aaggaaaaat agaaaaggct ggcacagaat acgtttttgg cccactggat tatcaaattt
     1741 cagaaggttt ttatcctaca ttgatagatt taatccgcct tgatgtgttt aaagatacgg
     1801 atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa actgctacaa gttttagaag
     1861 aagagttaga gagcgaccaa agaaaagtga atcaagtgag tgaagtcgaa gtaaatgaag
     1921 aagtaccatg ggaacgacca atctttccaa agaaggagtc aaaagaaaaa gagacagtct
     1981 atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa gagaacgatg
     2041 tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa gagacggacg
     2101 aagtagaagt tgatccagtt gaagctaatg aagagtcaac atggactgaa ggggattttc
     2161 aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaaca gagatacaaa
     2221 aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa tggatgaaag
     2281 aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc caaacctatc
     2341 aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca cttttagaac
     2401 agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat ttattagcca
     2461 cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg acggaacagt
     2521 cgtctaattg ggagaaagta aaagaggaac taaataaaga agaacagcga gtgattgaag
     2581 aagaagtaca aaaagtcaaa gagcggtacg aaaagaaacg cgacaatgct tattccttgt
     2641 gtgtagaaaa gatcaacaat tttactaaga cgaatcaaga agcgttatcg aaagaaaaag
     2701 aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga gttctaccgc ggtttacgta
     2761 cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta tatcaaacaa
     2821 cgactgaagt tcttatggaa aaacacgaac agatccagca agaattacaa gtacaaattc
     2881 caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa gaagaaaaac
     2941 aacggcaaaa agaaaaagag cgtgaacaat tagaaataag aaaacgggaa caggatcttg
     3001 cggaacaaaa aatggcgata gaaaaacaac ggttagagca agaaaaggaa aaagaagcag
     3061 ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg tcaagcacag ttgaattatc
     3121 tagcacaacc tcaaccagta gctgctgttc aaacgccagt gaacaaccaa gcaaatttaa
     3181 ctccttcacc ccagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct
     3241 tgcttctagg tggaggaaca gtatttgctt ttaatcatat caatcaatct tcacaggctg
     3301 attctgtagc cacaacggcg tcaatcaagg aagaagccca ggaacaagca agaaaagagt
     3361 atgaacaaca attagaagaa ttattgaatc gtaccaatca gtcaacaaca gagtcaagca
     3421 tggaaaatag caaagagagt gatcctagta aggctaaaga aggatcaagc gattcaactg
     3481 tacactcaaa cacttccgaa agttcagaat caaatgaggt gaaataatgc gtattagaga
     3541 aacaacgaga cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt
     3601 ctatcgctat actccttcag gagaagagct accagaaaaa catgacaaac gtatttctca
     3661 aaagattttt tatagtggct tagcttatct tcctttaaac tatgttgtta caagaatacc
     3721 agaagcattt catcgatttg agattattcc aggaagttta caaggagtga atatttttca
     3781 gttacaagaa atgccgcagt tattccattt tgattcaact ggtttttgga atcctttgac
     3841 cccaacaaca agtttaggca cacgtttagt tgtttctacg atttgtgttg ttccagctgt
     3901 acttatggga ttgaaaatgg acatgtggtt tcgtccgcta gcagatggac aaaaagggga
     3961 taatcgatta atgaccttaa aagaaatcca acagatttat ccgcaaatac ctgaaacaaa
     4021 aactactttt ccaggttatg gaggtattcc aatcacacat tacaaaaaat attggtacat
     4081 tcaaacagat actgttaata cgtgtattgt tggtacctct cgatcaggga aaggtcagat
     4141 tgaagtgtta gccaccattg acaatttatc tcgtgcagaa aaacaaagtt caatggtcgt
     4201 taatgatcca aaaacagaat tatatgttgg tagtaaagat gaactagaag ctagagggta
     4261 tgatgtatat gcctttaata tcaccgatcc actgcaatca atgagtgata atccattggc
     4321 actcatcgtg aaatactgga aacgaggaga catagatact gcaacacagt taaccaatac
     4381 tttcacaaat acgatttatt atgatgccaa cgcttcagat aacaaatact ttaacgaaaa
     4441 cgcgcagaaa gctgtcaatg cgcttatttt tgcgttgtta gaacaagctg atcaaacagg
     4501 agattactcc aaattgacac cgaataatct cgttgagctt ctttctgaga ttggcggttt
     4561 taattattat ccagatccag acaatgaatt caaacagttg aatgccttag atgaatttat
     4621 gaatcagttg ccacctggaa acattgcaaa aaaacaatat ggggcaacga aaattggatc
     4681 tgataaagcg aagggaaata ttttatcaac ggcaatcacg ggattgaatc catttactct
     4741 aacgaaaatt gcaaagatga cttctcaatc aacgattgat tataaatcgg ttgggtttcc
     4801 aaaatactta gatttgaaat tacatgaaag tcttttaaat caacgagtcg tagtagaatt
     4861 ttatcataag ggaaagaaat ttcatgaaga acgtgtcaaa gtaggctata aaggatttag
     4921 tgaaatcaat tttgattgtc atttagttgc aggtgatcaa gtttgtttaa aatacgatca
     4981 tcaaggaaaa gaatatgtgg cttggtatga attttctcaa agagtattga aagacgaaga
     5041 gggtaatatc gtttatcgga aaaaacgagg agaaacccat ttgccagaat tagaaaaaga
     5101 agccgttcta acattagatg aggaaaaaag taacttatta gtagagtcta ttcggatgca
     5161 ctataccgat aaaccaatgg ctatttttat gcttacacct gattatgatc catctaacca
     5221 tatcattgtt agtatttttc ttagtcagtt atataaagaa ttatctacgc aatgtgttaa
     5281 gactaaagga gataaatgtc accgcaggat tcattttatt ttagatgaat ttgggaatat
     5341 gccaccaatg gataatatgg aaggaatcat gacggtcaca gctggccgaa acatgttgtg
     5401 ggatctgttt attcaatctt atcagcaact ttatgcgaag tatggagaac aaaacgcagc
     5461 gattatcaaa gaaaattgtc agacacatat ttatattatg tcgacagatg ataataccat
     5521 cgaagaattt tctaaaaaag tggggaataa gacggttgaa caagagaaca gtaccatcaa
     5581 ttcattaggt atgaatactc atatcaatcg caatgttgat tcagatcgta tcttaactcc
     5641 tgaaagaatt tctacttttt tagaaggaga aacgattgta ctcgctcctt taaaacgaca
     5701 agatttgaaa ggtcataaaa tacggccgta tcctattttt aatcatgggg aaacgaatct
     5761 tccttatgct tatcaatata ttgaagattt caatccatcg tgggatatca atgatataga
     5821 catacattgt acacatgcgg acttggatct taaggcttta caaattgatt atgattattt
     5881 cctaccagat gaatatgcta gaaatctcta tcatggactg catgatcaat tagaagaaaa
     5941 aaacgaagaa aatatgtttt ataaaatcgt tcaatctttt gagaatgaga cggctaaaaa
     6001 attgttaatt caattttacg aagaacaagc agcagatcga cttcaagaat atgttgaaaa
     6061 gttgatcaaa aataaagaaa tttctgaaat gattgggaaa cggttgttat tggaatctga
     6121 aaaggtggtg aaagaatgat cattggagga attttttctg gcatagccga ctggtggtca
     6181 ggcttattcg gagaagggga tgcaaacccc aaaaccgtaa cgaaagtctt acaagattat
     6241 gggaattatc tgcagtatca agatgtattt agttggattt ggaacacttt tctgtgggga
     6301 ttgattaaag gattatataa tctcaatact ttactagaaa aaaccattta tcaaagcttt
     6361 gatttaaaag acttattgaa cgccgctggg gtaaatgcac tatttcaaag tttgattagt
     6421 aaggtattgg ctcttttttg tgtcgtcaca ttaatttatt taggcttgaa attttttatg
     6481 agtaaacacc caccaaaagt caaaaatatc ttggttaatt tatttatggc catgatcttg
     6541 atttttggtg gtagttcttt aatcgatgaa gggttaaata tcagtaaaag cttttacgga
     6601 gacgtcactg cagcaaataa agcagataaa acgggatcac ctgcttttca aattattcaa
     6661 aataatgtga tcgatgtcag tacgttacta gcaactgacc cgaataaagt agaatcccta
     6721 ccaacagata aaagaaatgc tttaacagcg aaaaatttta aaacagcgaa tatcaatggg
     6781 attattactc ctgaacaagc taaaggtatg gcaggtaatg ataaaagtgg gttatcagat
     6841 atacaaaagg atcggttaaa ggcattacaa taccgattgg aactagatga taatgggaaa
     6901 gaaattcctg ttcaaattag tgacgaaggc ttagcaaaat atgtctatac ttcagggtat
     6961 cgtcgataca gtagtaaacc aggtattatt ttagctggtg aatcttcttt ggcagtagcc
     7021 tatttattta tcctatttac gatcgttact tgcattattg agctggtatt taaaaaattc
     7081 tatttagtga ttgccgcgtc aacggacttt gagacaggtc aacggatgaa aagtgcgata
     7141 gaagatattt ctcaatcctt cttgttattg gcgtttacga ttttagaatt acgaatctat
     7201 accttaatgc tttcaggtat cggtgatttg catgcggcag gaaagatcaa tggattcttg
     7261 tatgtcgttg ctttgattgt actaaccatt gcgctattca aaggctctca atcagttacg
     7321 aaaattttcg gcgttgatac aagcctgaaa aacggcagta attctcttct tagcatgttt
     7381 gccttaggaa atattgcgaa aaatgtgggt agcggagcta aaaaccttgc aagtgctggt
     7441 aagagtggtt taagccattt aaatgatatt cgtaaaaatg gatttggcca tcgaaaagat
     7501 gttacccctg ataacgggga agaacaatca gacgataaag ctacaactgt acctaatagt
     7561 aagaaaacag tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa
     7621 ggtcttggtt acgtcaatga acgtgggtta aaaggaacag ctgaagatct tacagaaaaa
     7681 gccaaagaca ccttagaaga taaattagac ggcacgagag ctaaagaatt ggctgaagct
     7741 gtgaagaatc catccgaagc actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa
     7801 ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaagc taatacaaca
     7861 accgaaaaag aaaatcaaga aggtatagtg attccttcag tcaaagatca ccaagaagcg
     7921 ttgcactcta atgaaaattc gattgaagag aaaaattcca tagaagaaag aaattcagaa
     7981 gaaggaaaaa atggacagtt accaaaaact gatccagaac aatatcgcta taaacctaac
     8041 agtgaccttg aaaatgagaa gcgaatttca gatttatcaa aagtaaatca agaagacaaa
     8101 gcagaaaatg agggacagtt accaaaaact gatccagaac aatatcggta tagatctgtt
     8161 aactccgaaa atgaaaaacg catttcagat ttaccaaaac gaaatcaaga agtacaaacg
     8221 gatgaggatt ccatcgttct gccaaatgat aatggaatcg aagttggaac tgaaaaatca
     8281 acgaaaattg gtttacctaa ggcacaaaat ttaccaatga aagatagtcg tttgaatcaa
     8341 ttcaaggata atgatcaaag ttttgttcat caatcacctg caggtactgt tcaaatgaaa
     8401 ccaagtcgtt tgcctgaaaa tggaaatctg acaactgttt cttacaattt atctgaaagt
     8461 ggtgagacag ggaatgcgtc cgttcaaaat gttgtgacaa gagaagctgc accaaaacct
     8521 attcaaaacg ttcatacagg ttacaagcca gcaaatgtgg catttactgc accaacaaaa
     8581 ataaatgtga caagagaaac aaaaaacagg aacaatgcat tgaaagaaca actattgagg
     8641 gatatcaaac aggatatcaa accataaaag gcctacaagt taggcctttt ttctatgtat
     8701 gaaaaggaga aatcatttat ggataaatat agtaacatga aaaaccctcc aagatttagt
     8761 ggagaatgga agtttttcgc ttttttcttt ttagatctat taacgcttgg tggctgcttt
     8821 ttttgggcga aaatggtcaa taatcttgtc cacttaccac tcttttttgc actcttgaat
     8881 tatggcttat ggattgcgat agcgttagtg attgtttggc gaccctataa taacccaggt
     8941 caacgacaaa tttttgcgat tatcgagtct attttttttg cggacaagaa ccattatcat
     9001 gcgttagatc ctaaccgaaa ttatcatggt acgtataagg aggatgaata aatgcgagtc
     9061 aaaggacaaa attcagttga aaggaaatcc attttgcagt ttggaaaacc agaagagcgc
     9121 tctatcttgg acttatttcc atataaatcc tgtacagaag agggagtctt aattactaga
     9181 gaagatcatt ttcaacgtta ttatcgtgta gtttctacag acgtggaagg attaaatgaa
     9241 caagagaaac tagaacgaat gaatcagttg accaccgtca tgcgtacgta tgtgccaaca
     9301 attaagttag ttagtttgac cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg
     9361 caattattag ataaaaatcg tatggaacag tccttaggaa gaaatctaca acgattaaaa
     9421 aaatatgaaa agaaattagt cgaagaaatc caggaattga aacgagcaga acaagaacgt
     9481 cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa tcaaggattt acaaaccaga
     9541 aaccgtcagt taatgcgatc tagtggcatt ttagggttaa aaccactcta caaaaaagaa
     9601 ttgatcaaga ttctgtatcg aatgaacaat atgaatgatg agtaggaatg gagaagagag
     9661 aatgaaatat ccgttgatat ggttagaaaa agatgttccg aaggaagaga ccactagaaa
     9721 atttcctcac actttaagcc tgggtaagtt gggaaaaggg caatctttta accaagtaaa
     9781 gaatcaaaca agaagaggtg actgtacatg ttagaagcaa ttaaaacagc tttttcacca
     9841 caaaatgatc aaaaagtact tgatccatta aagcaactag gaaatcggga aatagaaatt
     9901 gtaaaatcca aagggtatga tttagatttt ttaaaacaag tgatgccaca aggtgggtta
     9961 aattttgatg aagaatatgt gacgtatggt aatggttatt cacgatgtgt gacagtttat
    10021 cactacacaa aaaatcctac gcttttttgg ttggccaatc tcatgaacaa tcctcataca
    10081 gtggccacac tcgatgttag cacagatgaa aagcaaaaag tgattgatca aatcaatcgt
    10141 gccttagatg aattaaatga tcgatcagat aatgaacgaa aagcgactga ccgaaataaa
    10201 agcgcggaag aatacctgga actagcggaa tatgctagaa aattgacttc taatggggaa
    10261 atttctaagc aagtcaaagt gagagtacat gtttacggaa acacgattca agaagtagat
    10321 gaacgagcgc aggaactgat cgaaaatctc aaagggatgg attatcgtgc ggtgacttat
    10381 ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca tgttcgatga acaagctgaa
    10441 tggttcggaa atatcaaagg gcaatcacta ccatcgttaa acattggcgg aggtttcccg
    10501 ttccaccacc aaacgctaaa agatccagga gggacacaat atggggaaac catgacaggc
    10561 ggcccatttg tctggaatgc gacgtataaa aatagtgtac ggttgtctac taataccttg
    10621 cttctaggaa tgatgggagc tggtaaatcg accgttctta aaatgatcgc ggaagcccat
    10681 ttagctgcag gagactttat ttggggcttt gaaaagggaa aagactttat tcctttctta
    10741 aaggaatata acggtattat ggtgcgttta gacggatctg acggaatgat caatccttta
    10801 gagatatttg ccacacggac gtatgatgaa gcatccagtt tatatgacga tggatcagtc
    10861 aaagatttga agatcaatga agcagcgtcc tatcaaacac acttagacaa agtggtctat
    10921 caggtacagt tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt taagacctac
    10981 ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg gctttacttc ctcaaattcg
    11041 cgttcaaata ctgatacgca agtcacaggg aaagatccag aagcttatcc gacgttcaaa
    11101 gaatttttag actttttagg acaactggaa ttaccaggtg cttcccaaga gaaaaagaat
    11161 cgaaaagaag aaatggaatc catcgtagaa tccttatgtg agacgtatgg catgattttt
    11221 gatggtcata gtacgattcg acaccttgac caacaacagt tggtcttttt tgacattgac
    11281 tctattacag gcttagatcg aggggtacaa caatgtcaaa tgtacatggc tatgacgttg
    11341 atttggaatc aggcattgat tcaaggaaga agacaacgac gtttaattaa agaaaaacaa
    11401 atggaagaac aagacttcaa acgcttcttg gcgatgattg atgagtgcca taatggtgcg
    11461 acctgttctg cagtgaaaag ctgtgggttg caagtagtct aaaaactatt gtaataactc
    11521 ttataacgag aagtcaaatg agtgggtaac gccacaaagg gcttccactg atactccgac
    11581 atgcaacaag tagtttatgc agaccaacgt tgtgtgaagc tcggtgaagt cggctgaagg
    11641 ataccgtaag gtaaaagaaa tagtgaatac cacgaaagtt ctctagaggt aaagggtgta
    11701 tccgataagt cgggtgtcca aaaaaactta atatggtgag tatgtacaaa cgtactgacg
    11761 aactagcgaa tgtactgtcc gaaaggcata gctcctagaa atgggagaga cagtgagcaa
    11821 aaaatcactc attgtgctaa cttgtggttg agtaaaagtc gttgttatga aagaccatgc
    11881 tgcgttacag gcgtaggaga agttaggggg caaaagctag catctaagtt aaggcatgtg
    11941 gatagatata agggaacagg ggaagccatc aacatggaga gtgcatactc gatgaagtgg
    12001 tagtaagaaa tatgtgaatg acaaataact tactttagtg atggtgaaag tagagtcacg
    12061 actgatgaag gttctgtaat ggaactggag gaacaggctc gattcgtctg taaaagtcat
    12121 gaaacccgtt caaccatggg attctcgtat gactaaaaag gtcacgagcc aattggagcg
    12181 tgataaacca tgacaaaaga aacaagtcca aaagatagga agttgcggca tgcagaatat
    12241 tatggcatga cggaaatctt cgataagata tactccaaaa gtttgaaggg agataatttc
    12301 aagaatctaa tgcagattat catttctgat aacaacattg tcctagcata tcgtaatatc
    12361 aagagaaatc acggtagtgt gacaagagga gaagatggag taacgattaa agatattgaa
    12421 aatctcaacc gtaatgaatt cgtagaaatt gttaggaagc gattcgcaca ttataaccct
    12481 cggaaagtaa ggcgtgtaga aattcctaaa tataatggta aattaagacc gttgggtatt
    12541 ccatcaatat gggatagaat cgcgcagcaa tgcatcttac aagtattaga accaatatgt
    12601 gaagcaaaat tcaataatca cagctatggt ttccgcccaa atagatcagc agaacacgca
    12661 atagcagatt gtatggcccg aatgaatcaa agtcacatgg aatatgtggt agatgtagat
    12721 atcaaaggtt tcttcgacga agtcaatcat actaaactca tgagacaaat ctggtctatg
    12781 ggtatacgag acaaacagct tctcttaatc attaggaaaa tgttaagagc acccgtagta
    12841 cttccaaatg ggaagttgat atacccaaca aaaggaacac cacaaggcgg aatacttagc
    12901 ccattacttg cgaacatcaa tctcaacgaa tttgactggt ggatagcaaa tcagtgggag
    12961 gaaaaagatg ccaaagagct aaaacatcat tttaggaaaa atggagcttg ggataaaggt
    13021 aatatctatc acaagctcag aaagagtaca actctaaaag aaatatacct agtgagatac
    13081 gcagatgatt ttaaaatttt ctgtagaaat tataatgaag cgaaaaaagt tttctatgca
    13141 actaaaatgt ggttacagga gcgtctaaag ttaccaatat ctgaagaaaa gtcaaaaatc
    13201 acaaatcttt caaaagaaga aagtgaattt ttaggcttta cgatgaaatt agtgaccaaa
    13261 ggtaacaaaa aggttgttaa tacgcatata ggtacaaaag cacaaagcat gattaaagtg
    13321 aaactaaaaa aacaaatcaa acgaattcaa aaaagtccta atagcaaaga tacctttatc
    13381 tatattggag tctataacag tatggttatt gggattcata attacttcaa aattgcgact
    13441 cattgtagtg tagcaatgcg aaaaattgca agagaactca gaccaagttt tgaaatacga
    13501 ctacgagaac aaggaatctc aaaagaagga caatacggaa agtacgaagg atacaagcct
    13561 tacctacggt caggacaaat acgatatata agagggtttc caatattacc agtagggtat
    13621 attcaacaca gacatccgat gtgtaaaaaa agaaatatta ataaatacac cgaagaaggt
    13681 agggaagcca ttcataagaa gcaacaaacc gtagaagaat ggaagatatc gtggttgtgt
    13741 agcaatccag tgcttagtaa gagagcaacg attgaatttg cggacaatcg aatttcaaaa
    13801 ttcatcgctc aaaagggaag atgtgcagta acaggtgagg agcttatctt atctgaaatg
    13861 cattgtcatc atattatccc ttatcatgaa agcaaaagtg atagctatga aaacctagtg
    13921 attgttactg aggaggttca tcgggtaata cacgctacac aaagtgaaac gattgaggaa
    13981 cttctaaaat atctaaaatt aaatccaaaa caaaaagaaa aactcaatga gttgagatta
    14041 aaagttggta acgaagaaat atcttaataa atatttgtag gacgggaaaa cagaatgtct
    14101 tttatagacg agggaacgcc gtatgcggtg aaagtcgcac gtacggtgtg gactggggga
    14161 aaagacggag ataatatcaa actcttacct atcagtatcg attaatccag attttattga
    14221 aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag caatgactgt
    14281 tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg ataacttctc
    14341 gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta tggatgaatc
    14401 tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag aattagaatc
    14461 cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag aaaccattcg
    14521 tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa aataatggaa
    14581 aaccaaaatg aaagtttaat caagcagtat gttaagcgtc gtgcaaagcg gcgcttattt
    14641 ttgtggttgt ttggtacttc tgcaggtttg attactattt tgatcactgt gttcgtgacc
    14701 ctatttctaa tacttgcggc gggctcgatt gataattcgg attcagattc aagcagtggt
    14761 ggcgaagctt ttacaggaga atattcggaa ggcttaccga tttataaaga aatcaaagga
    14821 agaggccctt tttctgatga gattgcgcaa tatgccgtag gtgcagcagt gaaatataaa
    14881 ttattaccaa gtgtgatcct ttcgcaatac ggttacgaat cagcgtttgg tacatcggcg
    14941 tccgcaagaa atgatctcaa ctattttggg attacttggt ttgatggttg tctgtttcct
    15001 aaaggaactg cacgaggaat tggtggcatt gaaggtggct ggtacatgaa gtttcccaac
    15061 tcaaaagctg cattttccta ttacggcttt atggtagcca cacaatccaa ttttaatgct
    15121 tgtgtaggaa ataaaagccc tggcgcaagc ttattgatct taggacgagg cggttatgcc
    15181 gctgcaggaa ttacggaaga tagtccgtat tatacaggtt gtatgtcaat tatcacttct
    15241 aataaattaa ccgaatatga tgaattcgcc atcaaacatt ggggtgaagg tggcaataac
    15301 aacggcacga ttactggtga atggacgaat ccattccctg gcagcagttt agataaaagt
    15361 tccttcagcg gtggtcaatt atttggaacg aatccaggcg gtgaattccg tccgaacggc
    15421 ttccatgatg gattagattt tggttcagtt gatcatccag gaagtgagat tcatgcggtt
    15481 catggcggaa aagtcgtgta cgttggcaat cctggtattt caggtttggg tgcgtgtgtg
    15541 atcgtgatta attacgatgg cttgaatatg gtgtatcaag aatttgcaaa tagtacagga
    15601 aattctcgag tgaaagtagg cgatcaagtg aaagttggtc aagtaattgg gatacgagat
    15661 acagcacact tgcatttagg atttactcga atggattggc gtcaagcaca aggacatgca
    15721 ttcattgatg atggtacatg gatcgatccg ttaccatttt tgaatagcag taaaaaatag
    15781 atcaggagaa aaaatatgga aaataaaacg ttaaaaatcg tcttaacagc tgcaattagc
    15841 gttagtttaa tcggcaatat tgcgttgttt tggcagctga aagaggaaca agcagcaaat
    15901 acctataaaa ttgaagaagt ggctaaaaac gcgtctgaga agctcaaaaa aaattcctct
    15961 gaggcacagg cgaagatcga caagttaaat caaaccatca atgagaaaaa ccaagaaatc
    16021 gatcagctga agcaacaaga tggcacaaaa gcaaaagagg aagtaactga agcacaaaga
    16081 aagacggcag aagagttcgg tcaattggcc attgataaat cattaagcag aaatgaatta
    16141 acggaaaaac taaaagatgt cgcaacccaa gaagtgatcg acaaattaag tcctacagac
    16201 gatgatcacc aacatgatga ttacggaagt tactccttta cattaggtga cgtgcaaact
    16261 tacgctcaaa caagcagtgt aaaagaggaa caaaattttg tcgtatttgt tgattataca
    16321 atcggcaatc cgcaattcaa agatgtcaaa ccacaaaaaa taaaaggtgg attaactgtc
    16381 actgaaaaac aagtagatgg tcaatggaaa gtcatagact ttagttattt cactcgttag
    16441 gaggaagaaa atgagtaaga aaaaaggaga aagacaacgt ttcaaaaaac gaaaaagtta
    16501 tcttagatgg ctgggaatct ttatgattct cggctttttg attcttgcta gtccgattta
    16561 tacgaatcag aaggtagata taccagatga ggtaagcacg aacgcctttt taaacacgaa
    16621 agagctgaca atggtttcaa aagaaaaagg agcgaaccag caattagttt ttgaatttgc
    16681 gatcgatgat gaagatcaaa gcttgttaaa agaactggct aatcttaatt atcgcgtaga
    16741 agtaaagaca gctaaagggg attatcaagc aatacgagcc aaagtgatta aggtgtctga
    16801 tgattatttt gcagtgaagc taacgcatgt accagaagaa tatatcgcca tgcgtttaac
    16861 cattattcct gaaaaaattg atccaaaagt ggatatgcaa gaaccacaag atctgatttt
    16921 ttatattcac gaagataagg tcaaagatca cgtgaaagac gaagattatg aacaacatgc
    16981 gatcaattac aaagtgaaag gatacaagaa agaacaaaaa gcagctcaaa aacaaatgga
    17041 atctttccaa gcaaccattg atttgaatga agaattagtg aagaagctaa aagatcaatt
    17101 gccttatcaa gtcgcagatg atcaggaaaa tactgaaaac aagattaata gctaccagca
    17161 agaaattgaa acatcgaaaa cgcaactgaa agatcaagaa gagatcattc aaaaacttca
    17221 agaaaaaatt gatcgtatga caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat
    17281 gaagcgaaca aacttggctg acaaagatac tttcgctgag cagaaagtat tgattaaagc
    17341 ctatctcaaa gttgtaaaag ctaaagaaat atgtcgagaa gtgaatttat ctaacacaac
    17401 aatatctgag ttgaagaatg gtcgaaagaa tttagatacc ttaaaattca gatcatttga
    17461 agctctatat gtagctgcta aaaaatattt tacagctgat aacaaataaa aaacaggagt
    17521 attttatgtg aaaaaagtat ataaaaaatg cttatgaatc agctatatca ccttaaccat
    17581 ttgttaatga gatgggagaa aacattaacc acgattctaa cgcaaacatc aagcacataa
    17641 aacatttaac actcctcctt tctggctgaa gatttcaacc aaacaggaag acagcgtatc
    17701 aattatagca tacacttttt cttaaattca tttttcatat gacaaaatat ttccttctaa
    17761 tcgacaaagt tagaaggatt ttttattgtc taaatacaca aataacctta atttttaaag
    17821 agcataagga aacgtgatat cactttctca cgaaaggtgt taggtaggct gtgcctacca
    17881 cgtttctaag aaagataaat gatatcaaaa ttttggattt gtgtgtagat ttgtgggtag
    17941 gatgaaagcg aatgtgggca gtaaaataag cgtttgtgtg tagcaaaatc aaaaaatgtg
    18001 tagggatatg tgtagctaca catatcccta cacataataa atttggagga aaatatggac
    18061 ttattaatta gaaatattga agaaaagtat attaataaaa ttgacaaacg ttgtgaagaa
    18121 cttacggtca agactggcaa aaaatggagt cgaaatcaat acctaaaagt tttgatcgaa
    18181 aatgattttg atcacgctct tctgaattat aaaaaagacc aatttgatcg gttgttagaa
    18241 aaatttgtgg atattcaaac ttataatacg aaaactttag aagaatatat tgcaacaaat
    18301 aatcaattaa ttggtttgtt aattgaataa taaaaatagt attgtgaatt tataaaagga
    18361 ggaaatcaat tttggcaaaa gcaacaagaa aacatgtata tctttacgat atgcatatcg
    18421 aagtacttga tgaaataatt tctactcatg aagatatatc taattatagt gaagcagtca
    18481 gacatttgtg tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca attcaaatta
    18541 aattgaatgc aatgggaaaa gaaatttcta ttttaaccga aatggtttca gagtttgcgg
    18601 atcttcattt gaaagataca ggaatactaa ttggtacaaa ttcaactgtt tatcaagaag
    18661 cagaagaacg tgtggaagga aaaattaaat ccaatcaaac caaaaaatat tcttctaagt
    18721 taaagaaacc agtgaatgaa tctgttcata ccagagtaga tccatttgat ccaacgaaga
    18781 ttctaaaaag ataaaagtag aaggagaacg tgatgaataa atcgcctgca gttattctta
    18841 cttgtcaatt taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa
    18901 gagaaaaagc actggaagaa atggaacgac gtacgcccga agaagaacag gaactgcaac
    18961 gaatcaagaa tgctttagag gatttttata ttcctgaagg agaaactttt tcggaaagta
    19021 agacggataa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
    19081 tcaattttga caagttagag gaacaagatt ttaccaaata tctttcttat atgacccgtc
    19141 actatgcgtt agaacaaaag aaagaactga cgcaaaggga acaaaaagaa taccaacggt
    19201 tgaaccaagc aatgaaaaaa tatcaaacag agccacataa aaaagcttca ggaaatcttc
    19261 ctggagtttt ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaaac
    19321 aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt atctctcacg
    19381 acaattcgta tcttgaaaaa atgggcttgt acgatcccaa aacagatacc ttagatgaag
    19441 aaggtttgaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
    19501 atgaaacagg ttactggatg gcaacgattc accgtaacac taagcatatc catattcatt
    19561 ttgcagttgt tgaaaaagaa aatacgcgta aaccgtatac tgagattgaa aatggtgtga
    19621 gttatatcgt accgaagggg aagagaagac aagcaacatt agacgcaatg aaaaccaatt
    19681 atgtacatga gttagaacgt ttctcttatg aaaaggatcg aatgatcggt atggagaaac
    19741 gaaatttatt gacacgaaaa agtgatttgc gtaacacctt aacaaaagta gtaaaagaac
    19801 cagcacgtta cgatcgacac gccatgaact tattaaatga agtatataaa accctacctg
    19861 aaaaacgttc ggaatggaat tatggagatg aaaatcgaac caagctttca ccatttacaa
    19921 ggggaaaact tgatgagtta accaaatatg ttttaacaaa agaacctgga tatcaagaat
    19981 atatacagat ttcaaaagca ttgaaagaag aagccaaaag cctttatggg gaaagcaaaa
    20041 gagaaacaaa gaatgcagaa aaaaacgccc tctttgattt aaaaaagcgc acaggaaatg
    20101 cgattttaac agaactgaag agacaggata gtcaaataaa accattaata gaaagtgtca
    20161 atcagatgca atctatttac aatgaacgat cctttcgaac actcaaacag ttgccaacaa
    20221 atgaatacaa gtatagttat caagaattcg taaagcgtag gaaacaattc caacaaagag
    20281 ttgtcacgca gcgaagcttg cgtcaattaa atcgaacaat taatgcacat gaagaaaaat
    20341 accacgcaag taaagcatat gaagagcaac agcgaagaat ccaaatggaa caagaaagac
    20401 aataggagga agagaaatga aacaatattt agatcaatgg aaagtcatag aaggatcact
    20461 aaaagaagaa cgaatcgaac aacttcctga ttgtttagaa aaagagcatt tgtttcagat
    20521 aagcgaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagttg ttgagtaccc
    20581 agctactggt gtgtattgtt gtaatcatgt gaagggtgaa aaatatttta ttattcaaga
    20641 atacgaaggt aagttagcgc cttattatac tacttgggaa ataaatgaag agggaatcaa
    20701 taatttccca tgtgaaagca ttgaagagtc tatttctctc acggaatgtt aaataaatag
    20761 agacgtaaaa gagtcctgaa cgtgattgtc caggactctt tttcaatgaa ttgttttccg
    20821 ccaattcact gagtggtgcg attcaatttt attatatcaa aagaaagtga gattccaaag
    20881 tgaagggagg aagaagaaat gactcagaca acgattggtg ttgtggttga aaaaagcagg
    20941 gataatctcg tttttgtgac agaaattcaa acaggtagag cttttgtagt cacagacaaa
    21001 gctgcaaaag cgtatcaaaa tggcaatatt ttaacgttaa atctgactac gaagatgttc
    21061 gttgatgcag tagaaaatta tccatttgtt tagacaaacg atattttaca aggaggaaac
    21121 ggacatggca gaagaaaaag aaccactaga taatagtcgg ttgggaaagt ccaaaagaaa
    21181 attagtacgg ttgcaaaacg aattaaataa tcaatttcaa aagctatttg atcaccaaaa
    21241 attgacaaat gggcaaccaa tgaatgacaa acgaaatggt cgttcatggt ttaaccagca
    21301 agaaaaaata gaaaataaaa tacaaagttt gagagaagag atcaagcaac aagaaaaaca
    21361 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
    21421 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
    21481 aggtgaatcg actttttcag ccgccacgat ccgaaaatat caacggaagt tggaaacatt
    21541 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
    21601 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
    21661 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
    21721 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
    21781 accaactgat tctctgaagg agaaagaaaa agatcatttc tatcgagtag aattcaacga
    21841 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
    21901 cgatcaactt aaaacgttgg ataaccaata cgctgcagaa gcaggttatt ataaattcta
    21961 ttttgaagaa gtgaagaacg gagaagtagt agatcagatc cgaatggatc taggggatgg
    22021 cgtcaacagt aatcacccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
    22081 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
    22141 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga tcaaacaaga
    22201 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attcttctga
    22261 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
    22321 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
    22381 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
    22441 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
    22501 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
    22561 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
    22621 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
    22681 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
    22741 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
    22801 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
    22861 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
    22921 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
    22981 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
    23041 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
    23101 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
    23161 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
    23221 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
    23281 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
    23341 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
    23401 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
    23461 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
    23521 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
    23581 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
    23641 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
    23701 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
    23761 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccactga
    23821 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
    23881 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
    23941 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
    24001 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
    24061 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
    24121 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
    24181 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagttaaa
    24241 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
    24301 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
    24361 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
    24421 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
    24481 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
    24541 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
    24601 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
    24661 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
    24721 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
    24781 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
    24841 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttat
    24901 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
    24961 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
    25021 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
    25081 atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgttta
    25141 caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
    25201 tgtacgatga aaaatggaaa caatgggcat tagaacattt gcctattttc cccaatcaat
    25261 accactatca tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa cgacagctgc
    25321 agtcagctga tacgattgtg atcgcgactg atagcgatcg tgaaggggag ctgattgcct
    25381 ggtcaatcat tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga ctttggatca
    25441 actccttaga aaaagaagcc atttatcagg gattccaaca gttacgagac gcagaagaga
    25501 cctatccaaa atttgaagaa gtacaagcac gtcagatcgc cgattggtta attgggatga
    25561 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agtttttctt
    25621 taggtagagt ccaaacccca acactctata tgatttatca attgcaggaa aagattagaa
    25681 acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat
    25741 ttgatgcgaa gcttgatcca aatgaaaccc aagcgacaca agaagcattt gaagcctatt
    25801 taaaagaaaa aggtgttcaa ttaggaaaac agccaggtac gatccttcaa gtcgaaacag
    25861 agaaaaaaag tgcggctagc ccacgtcttt tttctttatc gagccttcag tctaaaatga
    25921 atcaactcat gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag
    25981 gaaaatatct aagctatccg cgaacggata ccccttatat tacggaaggg gaatatgcgt
    26041 atctgttgga tcacttagac gaatacaagc attttttaaa agccgaagcg attccgactc
    26101 ctattcatac accgaatagt cggtatgtga ataataagaa agtccaggaa cattacgcga
    26161 tcattcctac aaaaacggtt atgacagcag cggcttttga acaactaagt ccactgcagc
    26221 aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aagtatacct
    26281 atgaagaaac aacgatcttg acgcaagttc agcagcttca gttaaaggca atcgaaaagg
    26341 tgccgctaga tctaggttgg aagaagttat ttggaaaaga gagcgacgga aaagagaagg
    26401 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggtg gatctacaag
    26461 ttcttgaaaa agaaaccaag ccaccacagc cttataccga aggtacgttg atcacggcga
    26521 tgaaaacagc tgggaaaacc gttgatagtg aagaagcgca atccatattg aaagaagtgg
    26581 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
    26641 atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg ctttgccaag
    26701 ccgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
    26761 tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat atccaaaaat
    26821 ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat tttggatcga
    26881 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
    26941 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagca tgtacgtctg
    27001 aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca gcgacacagc
    27061 tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
    27121 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
    27181 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa taatggaggg
    27241 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
    27301 ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc
    27361 agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg ttaatataaa
    27421 actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt aatgaaagac
    27481 aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga tgctagaagt
    27541 gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat agaaaaggta
    27601 gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct cgctcagagt
    27661 atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca agtaggaaca
    27721 tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc aaaggagaaa
    27781 ttagatcagt atgccattca accgcattac actaataaag aaaattttga cctgggagtc
    27841 acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga gcgaacgtta
    27901 tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga agcattaaaa
    27961 gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat ggatatttta
    28021 aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga gtaaagatga
    28081 atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa acatttaaac
    28141 ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat tgttgaatcg
    28201 ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa atacgggtta
    28261 gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact aacaaaagaa
    28321 aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg aattcgtttt
    28381 gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt tcaagcaaag
    28441 tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac ttcaggtgat
    28501 tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga taaaacgatt
    28561 catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc cacgtttagt
    28621 tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat tccgaaatta
    28681 gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa ggaaagaaat
    28741 aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt gaaagatgag
    28801 ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa agacatttct
    28861 ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga gcaagagaag
    28921 gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat ggagagataa
    28981 agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat ttacgttata
    29041 ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat ttcacgaatc
    29101 aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa ccagcagaaa
    29161 cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga attcaaacac
    29221 ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg gccgataact
    29281 tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat caatcgtcca
    29341 gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac aacaatggat
    29401 tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac
    29461 cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat aatttagaca
    29521 acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag tggctttctt
    29581 ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc aaatttacaa
    29641 tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa aacaaaatca
    29701 aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg gtagcagaca
    29761 gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct ataattttag
    29821 cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag taataaaaac
    29881 gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta attgtgtagt
    29941 tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat acaatattgc
    30001 cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt atgttagaaa
    30061 tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt agtcctaaat
    30121 gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt aggaagacac
    30181 aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc gttcgagaag
    30241 aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca agacaaaata
    30301 gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta ttgatttgcc
    30361 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
    30421 gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca aatcaataaa
    30481 gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc aaacatgcga
    30541 aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta tcatgcacca
    30601 catactgttg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta tcgtttgtat
    30661 caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattacat tttgattgat
    30721 gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta aaagaagtcg
    30781 ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct ttacttattc
    30841 cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg ctatttgaag
    30901 acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa acttatgaaa
    30961 tcgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaatatgg ctcaaaatta
    31021 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
    31081 gttcctatac catgaaacac gtgtacccaa aaaacattac aaggaaaaat tgtcagtcgc
    31141 agtggaagaa atgattgaat caggtgtcga aatgaatctc attgcgacgt actatcgaac
    31201 actggaagaa ctgaaaaaac aaaatggaaa atggtttttc caagccttac tatgcttgga
    31261 agtaggtgtc aaaccaagca ctatcaaacc ttccgaatat caagccttag aactgactta
    31321 tgcaaaattc gcacagatta agaaagctaa agcaatggct agcgactggc tagatacttt
    31381 tgaaagtatc gaaaaaaatg gtgcatttta tacgattgaa tcggaggaga aagataatga
    31441 ataaggtggt ttatcagcta acaacagaag atgtttcgca atttttaaca agcaaagaac
    31501 acttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
    31561 actttattca aagagtggaa gaacagttgt atatggaaag cgaaacaagt caaacgaagg
    31621 tcacagtcag ccataatcat ttagacgtgt ataaacgtcg tcctgaaacg gtaaaaaaag
    31681 gaacagagct tctacgacta gaaacagaaa aatacatcat gaatgtgtac ctagaaagga
    31741 gtgagctggt cgaaaacttt gtcagctaca cgatttgtag gaaacactat tactcagaat
    31801 cagaatatta ttcaattttc gagagcatgg aattaatgga agaagaaatg gaagagctaa
    31861 acagcaaaaa tgcgacggta gacgattatt accttgtatt tactaggttt ttgacgtgtg
    31921 ggacggaatc tatcgtgtta atgggaatgg attatgaact cgctgtaccc gaagcatttt
    31981 tgagggaata tgtagaagaa gtagataacc caggcgacga ctggtacacg actttaactg
    32041 attttttaga cacctacact tacgacgata cagaagaaat ccaaaatgaa cttgaccgta
    32101 aacgaatgcc atatcaacga tttgaaagga aaaaaggtaa ataaccattt ataccgaaaa
    32161 gtgaggatgt gtgaaataag aacaaaaaac cttgaaatta agggaatggt tcttatttcg
    32221 caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg gcaaagcccg
    32281 ataacctaag agaaagaagg agatccatat gaataaaatc attcctctat tgttcgtact
    32341 gtttgttggc gagatactta tgcgtcatgc tgcagctgta catttaaact tcgtagtatt
    32401 cttaggaata ctggtatttt gtttgatctt ttggcgattt gtattagccc ttgtgagtct
    32461 actattcaag atgattgcag ccatattttt catagggtta tttcgatgaa aggagagagt
    32521 actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg aaaagagaca
    32581 aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga ttatgtgtat
    32641 tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag cactaaaaaa
    32701 gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt cctactattt
    32761 agaatatttt ctatgtcctt tttattcc
//
LOCUS       DASJPH010000021        32635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJPH010000021 DASJPH010000000
VERSION     DASJPH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.021"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            352..1041
                     /locus_tag="U3995_000920"
     CDS             352..1041
                     /locus_tag="U3995_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796259.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="U3995_000921"
     CDS             1028..2236
                     /locus_tag="U3995_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1796260.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="U3995_000922"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="U3995_000922"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1796261.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U3995_000923"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U3995_000923"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1796262.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="U3995_000924"
     CDS             complement(6339..7034)
                     /locus_tag="U3995_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1796263.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U3995_000925"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U3995_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1796264.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="U3995_000926"
     CDS             complement(8233..8424)
                     /locus_tag="U3995_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1796265.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="U3995_000927"
     CDS             complement(8445..9335)
                     /locus_tag="U3995_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1796266.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="U3995_000928"
     CDS             complement(9322..10089)
                     /locus_tag="U3995_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1796267.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U3995_000929"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U3995_000929"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1796268.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U3995_000930"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U3995_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1796269.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U3995_000931"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U3995_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1796270.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="U3995_000932"
     CDS             14660..15043
                     /locus_tag="U3995_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796271.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="U3995_000933"
     CDS             complement(15211..15969)
                     /locus_tag="U3995_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1796272.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="U3995_000934"
     CDS             complement(15987..16805)
                     /locus_tag="U3995_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1796273.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U3995_000935"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U3995_000935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1796274.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U3995_000936"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U3995_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1796275.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="U3995_000937"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="U3995_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1796276.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="U3995_000938"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="U3995_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1796277.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="U3995_000939"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="U3995_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1796278.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="U3995_000940"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="U3995_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1796279.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="U3995_000941"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="U3995_000941"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1796280.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="U3995_000942"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="U3995_000942"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1796281.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="U3995_000943"
     CDS             26847..27146
                     /locus_tag="U3995_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1796282.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="U3995_000944"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="U3995_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1796283.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="U3995_000945"
     CDS             27734..27970
                     /locus_tag="U3995_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1796284.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="U3995_000946"
     CDS             28113..30086
                     /locus_tag="U3995_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1796285.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="U3995_000947"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="U3995_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1796286.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="U3995_000948"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="U3995_000948"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1796287.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9925 a         6383 c         6441 g         9886 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgag
//
LOCUS       DASJPH010000022        32590 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJPH010000022 DASJPH010000000
VERSION     DASJPH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.022"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            401..769
                     /locus_tag="U3995_000949"
     CDS             401..769
                     /locus_tag="U3995_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1796288.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            949..1890
                     /locus_tag="U3995_000950"
     CDS             949..1890
                     /locus_tag="U3995_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1796289.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1959..2690
                     /locus_tag="U3995_000951"
     CDS             1959..2690
                     /locus_tag="U3995_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1796290.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2834..5014
                     /locus_tag="U3995_000952"
     CDS             2834..5014
                     /locus_tag="U3995_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1796291.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            5034..5516
                     /locus_tag="U3995_000953"
     CDS             5034..5516
                     /locus_tag="U3995_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1796292.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5532..6293
                     /locus_tag="U3995_000954"
     CDS             5532..6293
                     /locus_tag="U3995_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1796293.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6424..7758
                     /locus_tag="U3995_000955"
     CDS             6424..7758
                     /locus_tag="U3995_000955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1796294.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8065..8550
                     /locus_tag="U3995_000956"
     CDS             8065..8550
                     /locus_tag="U3995_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1796295.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8543..9226
                     /locus_tag="U3995_000957"
     CDS             8543..9226
                     /locus_tag="U3995_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1796296.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9352..11997
                     /gene="polA"
                     /locus_tag="U3995_000958"
     CDS             9352..11997
                     /gene="polA"
                     /locus_tag="U3995_000958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1796297.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12042..12878
                     /gene="mutM"
                     /locus_tag="U3995_000959"
     CDS             12042..12878
                     /gene="mutM"
                     /locus_tag="U3995_000959"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1796298.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12842..13471
                     /locus_tag="U3995_000960"
     CDS             12842..13471
                     /locus_tag="U3995_000960"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1796299.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13523..13855
                     /locus_tag="U3995_000961"
     CDS             13523..13855
                     /locus_tag="U3995_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1796300.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14167..14658
                     /gene="nrdR"
                     /locus_tag="U3995_000962"
     CDS             14167..14658
                     /gene="nrdR"
                     /locus_tag="U3995_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1796301.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14681..16054
                     /locus_tag="U3995_000963"
     CDS             14681..16054
                     /locus_tag="U3995_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1796302.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16054..16980
                     /gene="dnaI"
                     /locus_tag="U3995_000964"
     CDS             16054..16980
                     /gene="dnaI"
                     /locus_tag="U3995_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1796303.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17109..17549
                     /locus_tag="U3995_000965"
     CDS             17109..17549
                     /locus_tag="U3995_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1796304.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17693..18394
                     /locus_tag="U3995_000966"
     CDS             17693..18394
                     /locus_tag="U3995_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1796305.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18391..19512
                     /locus_tag="U3995_000967"
     CDS             18391..19512
                     /locus_tag="U3995_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1796306.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19527..20759
                     /gene="pepT"
                     /locus_tag="U3995_000968"
     CDS             19527..20759
                     /gene="pepT"
                     /locus_tag="U3995_000968"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1796307.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGSPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20891..21799
                     /locus_tag="U3995_000969"
     CDS             20891..21799
                     /locus_tag="U3995_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1796308.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21834..22106
                     /locus_tag="U3995_000970"
     CDS             21834..22106
                     /locus_tag="U3995_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1796309.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22117..23163
                     /locus_tag="U3995_000971"
     CDS             22117..23163
                     /locus_tag="U3995_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1796310.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23414..26023
                     /gene="clpB"
                     /locus_tag="U3995_000972"
     CDS             23414..26023
                     /gene="clpB"
                     /locus_tag="U3995_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1796311.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26174..26860)
                     /locus_tag="U3995_000973"
     CDS             complement(26174..26860)
                     /locus_tag="U3995_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796312.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26838..27332)
                     /locus_tag="U3995_000974"
     CDS             complement(26838..27332)
                     /locus_tag="U3995_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1796313.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27352..28572)
                     /locus_tag="U3995_000975"
     CDS             complement(27352..28572)
                     /locus_tag="U3995_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796314.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28719..30554
                     /gene="lepA"
                     /locus_tag="U3995_000976"
     CDS             28719..30554
                     /gene="lepA"
                     /locus_tag="U3995_000976"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1796315.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30750..31661
                     /locus_tag="U3995_000977"
     CDS             30750..31661
                     /locus_tag="U3995_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796316.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        11011 a         5663 c         6983 g         8933 t
ORIGIN      
        1 gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggtcg tgagcctgcc
       61 gtttcactct gagtcacaaa gagcaatttc aaaaaatagc ttctcatgtt tgttgaatat
      121 tgtgtcttgt gaccgaggag ttgtcaggag aacaccgttt accagaggtt gtgacttgtt
      181 tttagtcacg acctcttttt ttatttattc catataaata cacattattt attccgaaaa
      241 gtgaaagcgc aaataacaaa aacaaccaac ttatttatta ttaaagaaaa gcgtatttaa
      301 gtaggacgat tctgttgaga gctttcgtag attttcccat tttcctatgg taaactgaca
      361 aagttgagaa tgttagaaca gatcgtttag gaggaaacag atgtttacga atgaaataaa
      421 aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg ttttggagag cagttggctt
      481 tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata attgaagtag gagttgagga
      541 cagtcaaacc tcatttctgt tatatgatcg agcatttgtt gagtcccatt cacctggcac
      601 agctactagt gccccatctt tgatgtttta cagtaaagat atctttggac tatataaaaa
      661 aatgcagacg caatcagtca cattaggtga tatggtacag cttggagaag aatatgtctt
      721 taactttgct gatcccgatg gcaactactt tgcggttaca ggtaaataat agaaataaag
      781 actttgtttt tctgtgcgaa acaggaaagc gaagtctttt taattcaaat aataatatta
      841 tgtaaacaaa aaactttcca tgcgtaatta tacacaagca aataaatcgt caaatgcgaa
      901 cgccttcttt atactgttta ggaacgaaaa gaaggaagga agaaatacat ggattatcaa
      961 gtactgcttt attacaatta tacaaaaatc gaagaaccag aggaatttgc caaaaatcat
     1021 ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga agaaggaata
     1081 aatggtactg tgtctggaac cgttgaaaac acgactgcct atatgaaagc aatgaaagct
     1141 gatcctcgat ttgctgatac gatgttcaag atcgatccaa gcgaaggaca tgcctttaag
     1201 aagatgtttg taagataccg tccagagctt gtaagtttaa agctagaaga ggacattgat
     1261 ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc tattttagat
     1321 gaacagacag tggtgattga tgcacggaat gattatgaat atgacttggg acattttcga
     1381 ggggctgtac gcccagatat acgcagtttt cgagagcttc cagactggat ccgcgagcac
     1441 aaagaagaat ttatggaaaa acgggtagtg acctactgta ctggtggcat tcgttgcgag
     1501 aagttttctg gttggcttgt ccgtgaagga ttcaaagatg tcggacagct gcatggcggt
     1561 attgctactt atggtaagga tcctgaagtc caaggagaat tatgggacgg caagatgtat
     1621 gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag ataaacaaat cgtcggtcgc
     1681 gactggtttg acggaacacc atgtgaacga tatatcaatt gtagcaatcc tgagtgcaat
     1741 cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg tagcatagag
     1801 tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg gaatctctcc agaagaagca
     1861 gaactgcggt ttacagcttt aattaaatag aaaacaatag ttttgattgt cgaccatttc
     1921 caatatgcgc tatactagcg ttgaggatgg tgatccacat gaaatcattg atttatgcta
     1981 ttggggatgt ccatggcaaa catgagcttt ttgaaaaact tattcactat tatgatccaa
     2041 agatccatca gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt acaaaagaat
     2101 gttttttatt aggaaagcaa ttagtcgaag aaacaggagc catttacctc cggggaaacc
     2161 atgaagaata ttttttgcaa tttctgcaat caccggagga ctggtatgcg ccttatatga
     2221 gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg gaggaatact
     2281 ccccaactga aatctcgatg atgatccaat cgagatattc agaactgatt aaatttttag
     2341 tccagcgccc cttgtattat gaatgggaaa attatctttt tgtccatgcc ggtgtggatt
     2401 tgacaaagaa agactggcgt aagacaagtc taagagactt tatttggata agggaaccat
     2461 ttcatgaagg gaaaaacaat acaggtaaga caattgtatt tggacatacg attacaccaa
     2521 tgctccatgg agatatgcag acaacagacc tctggattca ggatcataaa atcggaatgg
     2581 acggtggggc tgtattcgga gggtcattgc atggggttgt ttttgacaag gaaggtattc
     2641 ttcaagatat tgaatatcag aacaacgaag gaccttggca tggaatctga ctaatggaaa
     2701 gaatgaagga gctgtgactt tgttgcggct ctttttattt ttatagagaa tattgtttct
     2761 ttcagtagtt taacccagga taaaaagtat gatagaataa agaagcaatt tttagcagaa
     2821 aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc attcagcttg tacaaaaaga
     2881 actttcagac tatcgtccta agcagttgac tactgtactt aatttgttaa atgaaggaaa
     2941 cactgttcct tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg atgaagtcca
     3001 gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac gtaaaactga
     3061 agtgattcgg ctaatcgatg aacaagggaa actgactccc gagttagaag cagaaatcac
     3121 ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac aaaaacgacg
     3181 gacaaaagca acgattgcta aagaaaaagg acttgaacca ctcgcgaagt ggctgatgca
     3241 gcttacagat ggagaggtgc aatctgaagc agaaaaatat attgacaaag aaaaagaagt
     3301 ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt attgctgaac aagtcagcga
     3361 taatgcaaaa tttcggactt ggattcggtc ttatacgtat aacaaaggta tgtatgtcag
     3421 caatgtcaag gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt atgactttgc
     3481 agaacccgtc cataaaatgg tttctcatcg aatattagct actaatcgtg gagaaaaaga
     3541 agatattctg aaagtattct ttcaagtaga tgaagcagca attttagctt acttggatcg
     3601 ccaaatagtc aaaaatcctg cctctccttc ttcttcattc gtcagagaag cctatcagga
     3661 tagttataaa cgctttattc agccagcaat cgagcgagaa ctgcgcaatg aattgactga
     3721 aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc ttttgttgca
     3781 accgccacta aaaggtaaag ttgtcctggg atttgatccg gcttatcgta ctggatgtaa
     3841 actagcagtc gtagatgcga cagggaaagt tttagcaatc gaagttattt atcctcataa
     3901 acctgcagcg caagcaaaaa gagaagctgc tggaccagct tttatccaat taattaataa
     3961 atatcaagta gatatggtag ctatcggaaa tggtacagcc agtcgggaat ctgaactttt
     4021 tgtggctgag cagttgaaat ccgccgacca taaaacctat tacgctatcg tcaacgaagc
     4081 aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa gaatttccgc atctacaagt
     4141 tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa gatcctttag ctgaattagt
     4201 caaaatcgat cctaaagctg tcggagtagg ccagtaccaa catgacgttt ctcaaaaacg
     4261 tttggctgaa caattggatt ttgttgtgga aacagcggtt aaccaagtag gtgtggatgt
     4321 gaatacagca agcccgcagc tcttgcagca tatatcagga ttgaataaaa caactgccca
     4381 aaacattgtc atttatcgtg aagaaaacgg agaattcacc gcacgaactc aattgaaaaa
     4441 agtgccacgc ttaggaccaa aagcatatga acaagcaatc ggattcttgc gagtccctgg
     4501 tgggaaaaac attttggaca acacaggtat ccatcccgaa agttattcta ttgcaaaaga
     4561 aatattaact tcagtccaac tttcagaaaa agagcttggt acagaagaag cagtagaaaa
     4621 attgacacga ttatccgtag agaaactggc tgaaagccta tcagtcggag aagaaacgtt
     4681 agccgatatt ttagctgggc tgacacaacc tggacgagat atgcgtgatg agatgccagc
     4741 gcccttatta cgtacagatg tattgagtat ggaagacttg aaaccaggga tggaattgac
     4801 tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt gatatcggtg tgaagcaaga
     4861 cggacttgtc catatatcga aactcagcaa aaaattcgtc aagcatccta cagatgtcgt
     4921 gtcagtagga gatatcgtaa cagtttggat agaacaagtc gatacgaaaa aaggcagaat
     4981 cagtttgacg atgctttccc cttatgaaga gtaacaatga ggagtaacaa aaattgacac
     5041 aagaagaact gcagcagtta gtagaagaga tatcattaaa aagcttccag cttcctttca
     5101 agcaccaagc aatgttcaat ccacgcttac gtacaactgg cgggcgctat catttaaacg
     5161 atcatcattt agattttaat gcgaagctat ttgaagagtc agacgaagag acgattgcag
     5221 gaatcatcaa acatgaactt tgccattatc atcttcatct agctggaaga gggtatcagc
     5281 ataaagatat ggatttcaag tatcttttga aaaaaacagg agggttgcgc tatgcaccag
     5341 ccttgacgaa gaaacagata aagatggagt attatcgatg ccaagattgt tctgccacga
     5401 tctgccgcaa gagaagaatc gatgtacgca aatatcgttg tggaagatgt catggagaaa
     5461 tcaaatggat tgagacaaaa agagaagact ttaatgaagc acaaaaaatc agatgaaagg
     5521 atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa agactggaat cagctattaa
     5581 attttttagc cgcacctgaa gagggaaatt ttgaggaact gacggcacct acacttattt
     5641 tagctggaaa ctgtcttcct atcttagctg ataaagcagc acaaatgtat ttaaatggcc
     5701 aagtagatca attgtttttg gtcggagggg tggggcatgc gactagaatc ctttatgaga
     5761 attttgaaaa gcaaggattt cattttgaag atggcatgag tgaaagtgaa atctgtcgtc
     5821 agtatctaaa agaagtctat gatttgcccg ataaggcttt tctgatagaa tcgaagtcta
     5881 caaattctgg agagaacgcc actttttcac tggagattct tcattcctta ggagctgtac
     5941 cagaaaaaat cttattgatg aatgatccga cactgcaacg caggactcga gcaacttttg
     6001 agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc tgtgccattt gtgccggaaa
     6061 tccttcactt ttctgaagag atcatcttta cagcaaaaga actgaatcat caatggccta
     6121 aggaatattt ccatgcgcta gtattaggag aaatggaacg tctccatgac gatgagaacg
     6181 gctatggtcc gaaaggaaaa gattttattc ctcacattga tatatcagag gaagtttggt
     6241 cttcttacac cagaattaaa gagtcaataa aaacggattt ttctcgtact tgaccatttt
     6301 agcaagcgaa tcctcctttc caaataaatt tggttgaaat tttcaatcgt ttataagata
     6361 atgaggagcg tgttgaatgt aatagctaaa agaatggacg ctagataaga gaagaggatt
     6421 caaatggaaa atcaaaataa attgtatcat tttgttggta tcaaaggttc aggaatgagc
     6481 tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc aaggatcaga cattgaaaaa
     6541 tatttcttta cacaaagaga tttagaaaaa gcaaatatca cgattctgcc atttaacgca
     6601 gataatgtaa aaccagggat gacgatcatt gcaggaaatg catttcctga ttcacatgaa
     6661 gaaatccagc gagcaaaaga attaggtttg gaagtgattc gctatcacga ctttattggt
     6721 cattttatcc aaaattatac gagtatcgct gtaacaggct ctcacggaaa aacaagtacg
     6781 actggactgc tctctcatgt attgagtggt gtccgtccaa caagttatct aattggagat
     6841 ggaacaggac atggtgatcc gcaagcggaa ttcttttcat ttgaggcctg tgaatatcgc
     6901 cgtcatttcc ttgcctattc accagactat gcaatcatga ccaatatcga ttttgatcat
     6961 ccggattatt atacaagtat cgacgatgta tttacagctt tccaaacaat ggctggacaa
     7021 gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt tacgcaaatt aaaagcaaat
     7081 gtgccgattt attattatgg tgtcacagaa aacgatgata tccaagcacg aaacatcgaa
     7141 cgcacaactt ctggttctgc ttttgatgtc tatcatgggg atgaatttgt tgggcacttc
     7201 actgtaccag cattcggtaa acacaatatc ttaaatgctt taggtgttat tgcagtagct
     7261 tatttcgaaa aactggatct gaaagaagtt gcagaagaaa tgctgacttt cccaggagta
     7321 aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg tagtcgatga ttatgcgcat
     7381 caccctgcag agatcaaagc aacgattgat ggcgcacgcc aaaaatatcc agataaagaa
     7441 ataattgcag ttttccaacc ccatacgttt acgcgtacga ttgcattgat ggatgaattt
     7501 gcagaagcac ttgatttagc tgataaagtt tacttatgcg atatttttgg ttcggctcgg
     7561 gaagaacaag gaaacgttaa gattgaagac cttggagcga aaatcaaaaa aggtggagaa
     7621 gtcatcaaag aaaataatgt atctccattg ttagattatc atgatgcagt agtaatcttc
     7681 atgggtgctg gagatgttca aaaatttgaa caagcgtatg aaaaactttt aagcagcacg
     7741 acgaaaaatg tactgtaata caattactgc agtgtcctta taaaaataga aagaggggga
     7801 gtggcaaaaa tcttgccaag cccccttttt tgcaacttat gataaacggt gttcaaaagc
     7861 ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg agaagctgtt ttttcttggc
     7921 tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta taggaatctg
     7981 ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa tgtatgatta
     8041 aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact aggaaaaaca
     8101 ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga agataaccag
     8161 cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca acatgacttt
     8221 gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat ggggatcatc
     8281 acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa tttttctgtc
     8341 aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga ggtcataaaa
     8401 gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac gaacgaagat
     8461 cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt ggaaatagca
     8521 atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag gtatcaatcg
     8581 tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg cagtcacttc
     8641 ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata attttccact
     8701 aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag gcgtaaaagc
     8761 acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt tgaatggcat
     8821 aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag ctttcttgac
     8881 agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa gagatcttag
     8941 cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac tagtcttgaa
     9001 catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt tagtcgtcat
     9061 ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg tccagacaaa
     9121 cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt tggactttat
     9181 caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa gaaaaaaatt
     9241 catcagttgg ggctataccg aattatttgg tatggtcccg ctttttcttt tcgcattccg
     9301 caggattctt tgttagaatg aataagaact ttacagtaag gagttaagaa gatgacaaaa
     9361 aataaattat tactcgttga tgggaacagt gtggcttttc gcgcattctt tgcgcttcac
     9421 aattctcttg aacgtttcaa aaatagaaac ggtcttcata caaatgcaat ctatgcattc
     9481 aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct ggttgcgttc
     9541 gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg agtacaaagg tggaagatcc
     9601 aaaacacctg gcgaattcaa agaacaaatg ccatatatac gtgatttgtt gacaggacta
     9661 ggggtacagt attacgaatt gcccaattat gaagcagatg atattatcgg tactttggca
     9721 gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt caggtgatcg ggatttgact
     9781 caattagcta gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag cgatatcgaa
     9841 agctatacac cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa acagattatt
     9901 gacatgaagg gactagccgg agatgcctct gacaatatcc ccggtgtgac taaaatcggt
     9961 gaaaaaacag ctatcaagct attgaaacaa tatggctcag tcgaaggaat ttatgaacat
    10021 atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa agagcaggct
    10081 ttcttatcga aaaaattggc gaccatcaat acttcagcgc ctgtggatgt ctctatcgac
    10141 tcattgaagt atgaagggaa aaatctcgat aaactcgttc cgttttataa agaaatggat
    10201 ttcaatcagt tcctttctaa attgaatatc gtagaagaag aagtaaaaat ggatgatatt
    10261 ttgtttgaag tcgttcatga attcaaagag gaaatgttta ctacggatat ggcgttgtac
    10321 gtggaaatga tgggcgacaa ttatcataca gaggaaatcg tcggagtagc gtgggggaca
    10381 gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg aaaaccgagc gttccattct
    10441 tggatcactg atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac ttatgttgct
    10501 ttaaaccgct atactggaaa aacgaaagga atcgattttg atgtcttgct aggtgcatac
    10561 cttctcgata cgaatgaaaa aagcacagat atcgaaggaa tagctgctca ttacggatac
    10621 aacgatatcc aatcagatga agctgtatac ggcaaagggg cgaaaaaagg tttaccagaa
    10681 gaagaggaac tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa tgcgttgaca
    10741 ccaaaattga gccaagaact ggcagaaaaa aaccaagaag atcttttccg gaaaatggaa
    10801 ctgccattgt ctatcatttt ggcagagatg gaaataagcg ggatcaaagt agatgcgact
    10861 cgcttacaag aaatgaaggg cgagttctct gctcgcttaa gagagatcga acaaaaaatc
    10921 tacgaagaag ccggcgaaga attcaatttg aattcaccaa agcaattagg agtgatctta
    10981 tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa ctggatattc cacagccgta
    11041 gatgtactgg aacagcttcg tgaacaagca ccaatcgtag aggatatcct cacctatcgt
    11101 caaattgcaa aaattcaatc tacctatgtc gagggactgc tgaaagtgat ccaatcagat
    11161 ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa caggacgatt aagctctgtt
    11221 gatcctaatt tgcagaacat cccgattcga ttagatgaag ggagaaagat acgtcaggca
    11281 tttgttccta gagaaaaaga ctggctgatt tattcttctg actattcaca aatcgagtta
    11341 cgcgtacttg cgcatatctc aaatgatgaa catttgaaag ctgccttcct agaaggtcaa
    11401 gatatccatg ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga ggatgtcaca
    11461 ccaaatatgc gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg catctctgat
    11521 tacggacttt cacaaaatct aggaatcagc cgaaaagcag cacagcaata catcgatacg
    11581 tactttgaaa aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg cgaagcaaaa
    11641 gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat atctgccaga catcaattca
    11701 agaaattaca atatccgttc ttttgcggaa cgaacagcta tcaatacacc aatccaagga
    11761 agtgctgctg atattttgaa aatcgcgatg atcgaattgg ataaacgatt aaaagaaaca
    11821 ggtcttcaag ctacgatgct gttacaagtt catgatgaac ttgtcttcga ggtaccggaa
    11881 aaagaacttg aatcattaga taaattggtg aaggaagtta tggaacaagc tgtttctctt
    11941 catgtaccac tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc aaaataaaca
    12001 ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa aatgccagaa ttaccagaag
    12061 tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt caaaaagttc
    12121 aggtcttgtg gcccaagatc atcgagcaac cagagacacc catctttgag gcttctcttg
    12181 ttggcgaaac catccaatcc attggacgta gagggaagtt tttaattttt catctcgatc
    12241 attgtgaact gatctcacac ttgcgaatgg aaggaaaata tcaatttacg aaagaaaata
    12301 caccaatcga taaacatacg catgtacttt tcttttttga ggatggaagc caacttcgct
    12361 acaacgatgt acgaaaattt ggtcgtatga caatcgtcga aaaaggagca agtgcaactt
    12421 accgaggaat catgaaattg ggacctgagc ctttgccaga tagcttctta ttagcagatt
    12481 tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc attgctgcta gaccaacgtc
    12541 tagtgactgg tctaggtaat atttatgtag atgaagcatt atgggaagcg aagatccatc
    12601 cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga acaattacgt ctatcgatca
    12661 ttgatgtttt ggatcgtgca atagaagccg gaggtaccac gattcgcagt tatttgaatg
    12721 cattaggaga atcaggcgga tttcaagtag cactgcatgt ctatcagcaa actggcaaac
    12781 catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg gggacgcact
    12841 attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag gtttgacggg
    12901 tggaatagct accggtaaaa gtacggcagc caaagtcttt cagtctcatg gatttccttt
    12961 gatcgatggc gatgtgattg ccagagaaac tgttgagcct ggaacgcttg ctctgaggaa
    13021 aattgtatca gtgtttggcc aagaggtatt gcagcctgat gggcaactag atcgagggaa
    13081 acttgggatg attgtttttc catcaaagga aaaacggcaa aaacttgatc agctattaga
    13141 tccgtttatc cgaaaagcga taaaagatca aatcgcctct ctaagttcca atcatccttt
    13201 agtaattgta gatatcccat tgctctacga aggtcattat gaccactata tggatgcagt
    13261 agctgtcgtt tatactacgc cagaaactca gcttcagcgt ttgatgaaac gtaatcaact
    13321 gacgctagaa caagcgcagc agagaattag tagtcaactt ccaatcgaag aaaaaaagca
    13381 gcgtgcggat attttgttcg acaacaatgg aacaaaagaa aaacttgtgg gacaaatcga
    13441 aaattggctt tttgctaatc gctttctttg agtatctgtg tcaattttgc tatcctgagt
    13501 atatagaaag tgagggatag cgatgggatc attaaaaaat caaaaggata aagtcgtagg
    13561 taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa aatctaatcg atgaaggtcg
    13621 tacgcaatca gaaacagcag aagaaaaaga aaagcagtac caagcaagaa tcgatgaaat
    13681 agaagaccag caggcagaag aagaagcgaa gcggcaagat ttgactcaag aacgaaaaaa
    13741 tccgacacct gatcgtagta aaacaccgct cccagaaagt gaccacattc ccgatgagca
    13801 aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc gaagagaagt tataaaatag
    13861 taaaaaatcg agatgtgact tgaagcagat gagaaggtgt tcatctgctt caagtttttt
    13921 gtgctagatg actaaatcag cttttgtgta agttatttct cagtaatcaa ggtgatcagt
    13981 attggaaaat tgaaatttag taaggacagg ggacacaatt acgcatgaaa gtagaaaaaa
    14041 ttgttcttat atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg tttcgtgtta
    14101 taatacaact agttgtaaag aaagacacat cctttatata aaatgcgatg caagaggtga
    14161 gacaaaatga gatgtcccag atgtcaacat aataattcac gagtaatcga cagccgtcaa
    14221 gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc gttccgattc
    14281 actacatttg aacgaattga agctgctccg ttattagtaa tcaagaaaaa cggggaacgc
    14341 gaagagttca atcgcgataa aattttaaga ggactcatcc gttcagcaga aaaaagacct
    14401 gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt acgcagtctc
    14461 ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg tgatggaaga tttagttgat
    14521 ttagacgaaa tcgcttatat tcgttttgca agtgtttatc gccagttcaa agatatgtct
    14581 gttttcttaa aagagttgca agatatcgtg gataaagcaa aagaaaataa taaagagaat
    14641 aataaagaaa acaattaaga aacaaaaagg aggcatttcc ttggaaaatg cttggaaaga
    14701 gcttcaacca aaaaatattt accaaggaag aaaagcggct ccgctgagag aagacagcaa
    14761 gaacgccttg ctgtatcttt atcagccact aatcggcgga gaagccttga gtatctattt
    14821 caccctgtta tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga tgcatgcaga
    14881 tctgcttagt agtctaggct gcggtatccc gcaattttat gatgcaagaa aaaagctaga
    14941 aggaatcggc ttactggatg tttattttaa agaagatagt gatctgggac ctcgttttat
    15001 ttatgagctg ttagaaccac tcattcctaa agcatttttc aaagactctg ttctggcttt
    15061 tcttcttctg gaaaaagttg gtgaacgacg attccagaag cttacccaat tctttgaacc
    15121 taaaaggcat acagcagagg gatatcaaaa aatcacaaaa aaatttttag atgtttatcg
    15181 cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc gaatcgctga ataaatcttt
    15241 tgaagaatct gaatcagtag cattacttga agcagattcg acattcgatt ggcagttttt
    15301 gaatgattgg ctggcaaaaa aacatattgc aagtcttgat gatccaatca tcaaacaagt
    15361 cgacatgtat catcagcttt acggtttcga tgaattgaca cttggcgaac tgatcagtca
    15421 atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag ccattttggc
    15481 aatgcagaga cgtccggtga agcaaacaaa agaaacagtt gcggtttctg aaaacagtaa
    15541 tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt cagctgataa aagaagcaca
    15601 gagcattcca ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg gatatgtttc
    15661 taaacaagaa aactggttac tgcaagatct tgtttctcaa tcaggtcttt ccagtagcgt
    15721 gatcaatgtt ttgctgaatt atgtactagt gatcaagaat aatgctagct taaatgccag
    15781 ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa atcgccacag cagaagaagc
    15841 gatccagcac attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa agaagcaaac
    15901 aaattatcaa actggcaagc gaaatgttcg acgggaaaag cttcctgatt gggtcaacca
    15961 gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca gagattgacc gccgttttaa
    16021 agaatacctg gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa agaattgaag
    16081 aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc tgaagtctta
    16141 aatgatgcgg atgttcaagc ttttctaagc gcacaccaag aagaattgac acaagcggat
    16201 attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa atatcaaaaa
    16261 aacgatccaa cgatgatcgc tcccggctat gagccgaagc tgacgttgaa ttttcattat
    16321 gtggatgtca cttatgtacc aacggaagca ttgttagcac ggcaaagaca agaagaaatc
    16381 agaagccgag taaaagcaat ggatatgcca aaagatattc aatcggcttc ttttgaaaac
    16441 tttgaacgta cagatggacg aggatatgcg agcttagaag ccctagattt tgtagagaaa
    16501 tatactgctg atccaaaagg gtatcataaa ggactgtatc tagtaggaag ttttggtatt
    16561 ggaaaaacat atttattagg agcaacagcg agagaattag cgattgctgg attcaccacg
    16621 acgctagtcc attttccgac atttgccgtt gagatgaaac aggcaatcgg aaaagatcaa
    16681 gtggcagaaa agttagatgc agtgaaacgt tcacctattt tgatgatcga tgacattggt
    16741 gcagatgcga tgagtagttg gatacgagac gaagtattcg gtgttatcct tcaatatcgg
    16801 atgcaggaac agttaccaac cttcttctca tcgaatttta caatggatga attggaacag
    16861 catttgtccg tcactcaacg tggagatgaa gagccgctaa aagcgaaacg gataatggaa
    16921 cgcatacgtt atttgacaaa agaaatcgaa atgactggtc gcaaccgacg caatggttga
    16981 acagttgaca agtccaactg acatttgacc tagtagtaaa agcaggtcag cgaatcatta
    17041 tatggacaga aatgaccgga gacaatcacc gcaacaagta atgcagaaac ttaaacggag
    17101 gattttttat gagggctatt ttttctagta tatctgtagt tggtctattt tttcttttag
    17161 caggctgcgg tagcgcagat agcaacacag gttctgttga ttcgattgat gcgggtcaaa
    17221 cagtaaaaac aagcagtacg aaaacggaga gacaaagcat atttgtcgga acgattgaaa
    17281 gtatcactca agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc gatgtcatgg
    17341 ccaaagaaga tccagcgaat atgggaacgt cattcagcaa tgacggagtg atcttaaatg
    17401 ctactgctga aaaaataagc gtggacataa atgaattgaa aatcggtgac aaagtccaat
    17461 ttacattagt tgagatgccg atcatgacga tgtctatccc accgcaagtt cctggagatt
    17521 cgattattga tgtttcattg aacaaataac caataacatt ttttcaaaaa taagaaaatg
    17581 ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt tttattccta
    17641 gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag ctatgaacca
    17701 tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat ccgctcgttt
    17761 agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa gaggaaaaat
    17821 cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac gtaagcaagt
    17881 caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc tagatgctat
    17941 ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt cactgatccg
    18001 agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac gtttgatcct
    18061 tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc agtataaaat
    18121 ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg tagccgaaaa
    18181 agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat ttctgcttga
    18241 agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa gaaaagctat
    18301 ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa tcgagcagga
    18361 aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac tttcatccaa
    18421 aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt cggactgcat
    18481 atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt tcgtccagaa
    18541 gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca tcctccaatc
    18601 tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta tgcagatttg
    18661 ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat ttgggatgga
    18721 ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt agtaaagact
    18781 catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc acagaaaatg
    18841 cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg tacaagtttt
    18901 acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc gattgggaaa
    18961 gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc ggaaactatc
    19021 gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc tgcttatgat
    19081 cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg tgaattgccg
    19141 caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca actagatgga
    19201 ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc gatttgcggt
    19261 ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt ctatatcacg
    19321 ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat tgcaattgat
    19381 cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt tgaatcttgg
    19441 aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac aaacccattt
    19501 caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt tttttacgat
    19561 atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg actcagacac
    19621 aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta gctgatgttt
    19681 tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt gatcatgaag
    19741 tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca gtaggagtca
    19801 atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat gcggaagaac
    19861 gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc cacacattga
    19921 tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt gcggaaatca
    19981 tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc atcaaagtgg
    20041 cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt gagcaattcg
    20101 atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag tatgaaacgt
    20161 ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca gggacggcaa
    20221 aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag cttccagccg
    20281 atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg gcattggatg
    20341 ggagtcctga agaagcttct atgagttata tcattcgcga tcatgatcgt gaaaaattcg
    20401 aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga tttgatcaag
    20461 aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc attgaaaaag
    20521 atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc tctccagtga
    20581 tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt attccgacgc
    20641 caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt tctttacaag
    20701 taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca aagagttaaa
    20761 atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt catctgtcta
    20821 aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa gagtaaatag
    20881 gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga aagatgaggg
    20941 tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag aattatatgt
    21001 agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga aagcagaagc
    21061 catagaagct gatactttag taattatagg tggagacggc acgattcatc atatcgtcca
    21121 agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg gaactgtcaa
    21181 caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg acgtgatcct
    21241 ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca ttatctctac
    21301 actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag aaaaacagcg
    21361 ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga agaaaaaata
    21421 tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac tgttgacagt
    21481 cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga ccctagatga
    21541 tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta ttttttattt
    21601 gcctagaatc atgagaggca aattctatac gatccccggc attacttatt tcacagccaa
    21661 acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg gagacacgac
    21721 agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt attcaccaaa
    21781 gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga gtaatggatg
    21841 tattgttaca caatcctttg tggttttacc ttttcattgt gaatttttac ttattttgtc
    21901 tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct gagaataatt
    21961 tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga aaagttttcc
    22021 atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta cttgttgatt
    22081 tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg aacgaaaact
    22141 aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta ttatttaatc
    22201 aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt tgttttggca
    22261 gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat caaagaaatt
    22321 gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc tagctatatt
    22381 gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat ttatttttat
    22441 aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca tatgattatg
    22501 tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt ctctcttttt
    22561 tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt gacgatcgtg
    22621 attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt ttttgtcagc
    22681 ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac atgcaatcag
    22741 cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac tgcgctattg
    22801 cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc ttatttgatt
    22861 ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat cagctttgca
    22921 gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga atttgtctat
    22981 ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct tttactgtat
    23041 cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat tgctgaacgg
    23101 cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga aaatcaaggg
    23161 taggtctttt ataagatcta ccctttttat caaatcaaag aaaaacgtta tggataagat
    23221 aattttacgt ttgtttggct ctttttttat atacatacaa tcaattttgt ttttttaaga
    23281 tttatataag tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc
    23341 aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg
    23401 agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga
    23461 agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa
    23521 aatcttttta caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt
    23581 cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg
    23641 aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga
    23701 ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt
    23761 gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa
    23821 agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca
    23881 agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag
    23941 cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct
    24001 ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc
    24061 aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga
    24121 taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga
    24181 atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct
    24241 cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga
    24301 tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac
    24361 aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca
    24421 aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa
    24481 agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc
    24541 tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat
    24601 cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca
    24661 agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga
    24721 tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga
    24781 agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc
    24841 aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga
    24901 tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa
    24961 ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga
    25021 aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga
    25081 aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca
    25141 agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga
    25201 cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga
    25261 attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga
    25321 tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta
    25381 tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat
    25441 cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt
    25501 cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt
    25561 tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg
    25621 aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc
    25681 agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt
    25741 gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat
    25801 cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc
    25861 atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa
    25921 agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg
    25981 acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat
    26041 aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg
    26101 ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc
    26161 acaacctctt ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct
    26221 catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg
    26281 tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag
    26341 aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact
    26401 cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac
    26461 tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg
    26521 tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca
    26581 aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga
    26641 ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg
    26701 caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg
    26761 tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt
    26821 gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct
    26881 gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa acctattgta
    26941 tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc
    27001 gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc
    27061 gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa
    27121 gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg
    27181 tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc
    27241 tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa atcactttta
    27301 gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata
    27361 atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc
    27421 atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa
    27481 gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg
    27541 gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg
    27601 atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa
    27661 ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact
    27721 atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat
    27781 catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata
    27841 gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg
    27901 ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca
    27961 atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt
    28021 gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac
    28081 tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat
    28141 ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg
    28201 cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt
    28261 agcatattct ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg
    28321 aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca
    28381 tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt
    28441 ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc
    28501 attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc
    28561 tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc aaaattctac
    28621 aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta
    28681 taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag
    28741 aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta
    28801 cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc
    28861 agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg
    28921 tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc
    28981 cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg
    29041 ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag
    29101 cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag
    29161 atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg
    29221 tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat
    29281 atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta
    29341 tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac
    29401 ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg
    29461 tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca
    29521 cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca
    29581 atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc
    29641 tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat
    29701 tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc
    29761 gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat
    29821 tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg
    29881 gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg
    29941 tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa
    30001 tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc
    30061 gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat
    30121 tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa
    30181 gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta
    30241 tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac
    30301 taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg
    30361 cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg
    30421 tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa
    30481 tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg gatgagcaag
    30541 atagtgtgaa ataacttata atcaatcgaa aaacctatta aataaacctt tttgagtaga
    30601 tgcaacactg aggattatct gtataaaaga ttttaaactg tcatttggtt atcacggcga
    30661 tactaaaatt aacaaaattg gaatattttt cttgaagatt tttagctgtc agtatatgat
    30721 gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga agggttctta
    30781 aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta gcaggtattt
    30841 cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa gacgtagata
    30901 atactctaag caagattctg aatatagaaa ctctaataaa agatgaggaa gataagattg
    30961 agattctcat cggtaaacta attgcataca gagatatgaa taagttactt aacaaagagt
    31021 tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat agaaagagaa
    31081 atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat gaaaaagaaa
    31141 ttttgaagga gattaatcgt gatagtgaga tatttagttt atcaaaagac gataaaattg
    31201 ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt gaaaaatttg
    31261 gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa aaaggtatta
    31321 atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc actgttcctt
    31381 accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga gttagcgatt
    31441 ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg aaagattatt
    31501 atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa tggtccatag
    31561 ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa tatgcttata
    31621 taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt acgtcgatac
    31681 ttattaagtt atcgacgttt tttattatag aaaaaattta taaaaggagg tttcttgata
    31741 tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt ttctaagtaa
    31801 ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaac taactgttat
    31861 gaataacagc tagataaaat gggctatgta tgttatccaa caatttagta atcactagaa
    31921 gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt gtttccaaat
    31981 caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca
    32041 taccatggag attcggagat ctgtgaggga atacttacgt ggttaatatg tcagttgtgt
    32101 tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag aaaatgaaac
    32161 caacaagaag atagggttat ttatacagaa ttggggatct tttctggaaa agtaatcgga
    32221 taatgcgaga ttcgtacggt cttttttaaa tttctctaaa aaaaacggca tcgaatgggt
    32281 tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaatga ttctatttat
    32341 ctttttacat ctatcgtttt ttcaagtagc cgcaaattat atcagcattc aacaattttt
    32401 gtatacaagt tctagtaaaa ggatctgaag aaaaagttgg ttgttgtgaa gataaaaaaa
    32461 gaatcaggtt ttactgagtt tttcttcaaa atttgtgtta ttaggttagg acaaagataa
    32521 gaagaagtat gaagatttaa aaaaggaatg aggaaaagat agtaatttcc gattattgga
    32581 gtttttttat
//
LOCUS       DASJPH010000023        32497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJPH010000023 DASJPH010000000
VERSION     DASJPH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.023"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="U3995_000978"
     CDS             complement(<1..563)
                     /locus_tag="U3995_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN1796317.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="U3995_000979"
     CDS             complement(750..1400)
                     /locus_tag="U3995_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796318.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U3995_000980"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U3995_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1796319.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="U3995_000981"
     CDS             complement(2912..3910)
                     /locus_tag="U3995_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1796320.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U3995_000982"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U3995_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1796321.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="U3995_000983"
     CDS             complement(4926..5657)
                     /locus_tag="U3995_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1796322.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="U3995_000984"
     CDS             complement(5661..6926)
                     /locus_tag="U3995_000984"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1796323.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U3995_000985"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U3995_000985"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1796324.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U3995_000986"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U3995_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1796325.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="U3995_000987"
     CDS             12268..13770
                     /locus_tag="U3995_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1796326.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="U3995_000988"
     CDS             13772..14509
                     /locus_tag="U3995_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1796327.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="U3995_000989"
     CDS             complement(14680..15873)
                     /locus_tag="U3995_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1796328.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="U3995_000990"
     CDS             complement(15952..16158)
                     /locus_tag="U3995_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN1796329.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="U3995_000991"
     CDS             complement(16151..17293)
                     /locus_tag="U3995_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1796330.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="U3995_000992"
     CDS             17805..18440
                     /locus_tag="U3995_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1796331.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="U3995_000993"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="U3995_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="U3995_000994"
     CDS             18916..19032
                     /locus_tag="U3995_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1796332.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="U3995_000995"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="U3995_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1796333.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="U3995_000996"
     CDS             21086..21253
                     /locus_tag="U3995_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1796334.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="U3995_000997"
     CDS             complement(21313..21666)
                     /locus_tag="U3995_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796335.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="U3995_000998"
     CDS             22170..22589
                     /locus_tag="U3995_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1796336.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="U3995_000999"
     CDS             22615..22821
                     /locus_tag="U3995_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796337.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="U3995_001000"
     CDS             complement(23049..23222)
                     /locus_tag="U3995_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796338.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="U3995_001001"
     CDS             23198..23863
                     /locus_tag="U3995_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796339.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U3995_001002"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U3995_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1796340.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U3995_001003"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U3995_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1796341.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U3995_001004"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U3995_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1796342.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U3995_001005"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U3995_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1796343.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="U3995_001006"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="U3995_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="U3995_001007"
     CDS             complement(30257..30484)
                     /locus_tag="U3995_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1796344.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="U3995_001008"
     CDS             30968..31558
                     /locus_tag="U3995_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1796345.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="U3995_001009"
     CDS             31748..32461
                     /locus_tag="U3995_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796346.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DASJPH010000024        31815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJPH010000024 DASJPH010000000
VERSION     DASJPH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.024"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..328)
                     /locus_tag="U3995_001010"
     CDS             complement(26..328)
                     /locus_tag="U3995_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1796347.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="U3995_001011"
     CDS             complement(353..2446)
                     /locus_tag="U3995_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1796348.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="U3995_001012"
     CDS             complement(2935..3699)
                     /locus_tag="U3995_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1796349.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U3995_001013"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U3995_001013"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1796350.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="U3995_001014"
     CDS             complement(4704..5780)
                     /locus_tag="U3995_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1796351.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="U3995_001015"
     CDS             complement(5804..6397)
                     /locus_tag="U3995_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1796352.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U3995_001016"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U3995_001016"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1796353.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="U3995_001017"
     CDS             complement(8067..8366)
                     /locus_tag="U3995_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1796354.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U3995_001018"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U3995_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1796355.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="U3995_001019"
     CDS             complement(10121..11032)
                     /locus_tag="U3995_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1796356.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="U3995_001020"
     CDS             11152..12009
                     /locus_tag="U3995_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1796357.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="U3995_001021"
     CDS             complement(12196..12558)
                     /locus_tag="U3995_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1796358.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="U3995_001022"
     CDS             12904..14343
                     /locus_tag="U3995_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796359.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="U3995_001023"
     CDS             complement(14350..16902)
                     /locus_tag="U3995_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1796360.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="U3995_001024"
     CDS             complement(17016..18347)
                     /locus_tag="U3995_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796361.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="U3995_001025"
     CDS             complement(18313..19791)
                     /locus_tag="U3995_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796362.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U3995_001026"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U3995_001026"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1796363.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="U3995_001027"
     CDS             22401..23828
                     /locus_tag="U3995_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796364.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="U3995_001028"
     CDS             complement(23920..24285)
                     /locus_tag="U3995_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1796365.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="U3995_001029"
     CDS             complement(24282..24647)
                     /locus_tag="U3995_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1796366.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="U3995_001030"
     CDS             complement(24839..25087)
                     /locus_tag="U3995_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796367.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="U3995_001031"
     CDS             25086..25214
                     /locus_tag="U3995_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1796368.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="U3995_001032"
     CDS             25271..26965
                     /locus_tag="U3995_001032"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1796369.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="U3995_001033"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="U3995_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27065..27238
                     /locus_tag="U3995_001034"
     CDS             27065..27238
                     /locus_tag="U3995_001034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796370.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="U3995_001035"
     CDS             27616..28749
                     /locus_tag="U3995_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1796371.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="U3995_001036"
     CDS             28780..29100
                     /locus_tag="U3995_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1796372.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="U3995_001037"
     CDS             complement(29129..29593)
                     /locus_tag="U3995_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796373.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="U3995_001038"
     CDS             complement(29725..30978)
                     /locus_tag="U3995_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1796374.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="U3995_001039"
     CDS             complement(31007..31324)
                     /locus_tag="U3995_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1796375.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="U3995_001040"
     CDS             complement(31350..31670)
                     /locus_tag="U3995_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1796376.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         9604 a         6605 c         5499 g        10107 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaaca
//
LOCUS       DASJPH010000025        31487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJPH010000025 DASJPH010000000
VERSION     DASJPH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.025"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..543)
                     /locus_tag="U3995_001041"
     CDS             complement(76..543)
                     /locus_tag="U3995_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1796377.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="U3995_001042"
     CDS             complement(663..2201)
                     /locus_tag="U3995_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1796378.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="U3995_001043"
     CDS             2281..2967
                     /locus_tag="U3995_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1796379.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="U3995_001044"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="U3995_001044"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1796380.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="U3995_001045"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="U3995_001045"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1796381.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="U3995_001046"
     CDS             complement(4453..5583)
                     /locus_tag="U3995_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1796382.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="U3995_001047"
     CDS             complement(5583..7766)
                     /locus_tag="U3995_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1796383.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="U3995_001048"
     CDS             complement(7763..8788)
                     /locus_tag="U3995_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1796384.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="U3995_001049"
     CDS             complement(8833..9660)
                     /locus_tag="U3995_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1796385.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="U3995_001050"
     CDS             complement(9657..10724)
                     /locus_tag="U3995_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1796386.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="U3995_001051"
     CDS             complement(10750..11586)
                     /locus_tag="U3995_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796387.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="U3995_001052"
     CDS             complement(11583..12452)
                     /locus_tag="U3995_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796388.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="U3995_001053"
     CDS             complement(12522..13805)
                     /locus_tag="U3995_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1796389.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="U3995_001054"
     CDS             complement(13819..15504)
                     /locus_tag="U3995_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1796390.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="U3995_001055"
     CDS             15779..16804
                     /locus_tag="U3995_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796391.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21073)
                     /gene="rpoC"
                     /locus_tag="U3995_001056"
     CDS             complement(17399..21073)
                     /gene="rpoC"
                     /locus_tag="U3995_001056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1796392.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="U3995_001057"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="U3995_001057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1796393.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="U3995_001058"
     CDS             25476..26444
                     /locus_tag="U3995_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1796394.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="U3995_001059"
     CDS             26462..27367
                     /locus_tag="U3995_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796395.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="U3995_001060"
     CDS             27364..28170
                     /locus_tag="U3995_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796396.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28306..29295
                     /locus_tag="U3995_001061"
     CDS             28306..29295
                     /locus_tag="U3995_001061"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1796397.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="U3995_001062"
     CDS             complement(29612..30136)
                     /locus_tag="U3995_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1796398.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31487
                     /gene="scm"
                     /locus_tag="U3995_001063"
     CDS             30423..>31487
                     /gene="scm"
                     /locus_tag="U3995_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1796399.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8832 a         6798 c         5930 g         9927 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg taaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgtcaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DASJPH010000026        30974 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJPH010000026 DASJPH010000000
VERSION     DASJPH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.026"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(507..1061)
                     /locus_tag="U3995_001064"
     CDS             complement(507..1061)
                     /locus_tag="U3995_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1796400.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1187..3116)
                     /locus_tag="U3995_001065"
                     /pseudo
     CDS             complement(1187..3116)
                     /locus_tag="U3995_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3422..4138)
                     /locus_tag="U3995_001066"
     CDS             complement(3422..4138)
                     /locus_tag="U3995_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1796401.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="U3995_001067"
     CDS             complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="U3995_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1796402.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="U3995_001068"
     CDS             complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="U3995_001068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1796403.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7608..9068)
                     /locus_tag="U3995_001069"
     CDS             complement(7608..9068)
                     /locus_tag="U3995_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796404.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9058..10353)
                     /locus_tag="U3995_001070"
     CDS             complement(9058..10353)
                     /locus_tag="U3995_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1796405.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10685..11362)
                     /locus_tag="U3995_001071"
     CDS             complement(10685..11362)
                     /locus_tag="U3995_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1796406.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11392..12483)
                     /locus_tag="U3995_001072"
     CDS             complement(11392..12483)
                     /locus_tag="U3995_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1796407.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(12584..13018)
                     /locus_tag="U3995_001073"
     CDS             complement(12584..13018)
                     /locus_tag="U3995_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1796408.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="U3995_001074"
     CDS             complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="U3995_001074"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1796409.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15056..16951)
                     /locus_tag="U3995_001075"
     CDS             complement(15056..16951)
                     /locus_tag="U3995_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1796410.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17009..18784)
                     /locus_tag="U3995_001076"
     CDS             complement(17009..18784)
                     /locus_tag="U3995_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1796411.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18834..20330)
                     /locus_tag="U3995_001077"
     CDS             complement(18834..20330)
                     /locus_tag="U3995_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1796412.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20524..22146)
                     /locus_tag="U3995_001078"
     CDS             complement(20524..22146)
                     /locus_tag="U3995_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1796413.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22474..23787)
                     /locus_tag="U3995_001079"
     CDS             complement(22474..23787)
                     /locus_tag="U3995_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1796414.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24032..24256)
                     /locus_tag="U3995_001080"
     CDS             complement(24032..24256)
                     /locus_tag="U3995_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796415.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24276..24698)
                     /locus_tag="U3995_001081"
     CDS             complement(24276..24698)
                     /locus_tag="U3995_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1796416.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(24840..25550)
                     /locus_tag="U3995_001082"
     CDS             complement(24840..25550)
                     /locus_tag="U3995_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796417.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25568..26065)
                     /locus_tag="U3995_001083"
     CDS             complement(25568..26065)
                     /locus_tag="U3995_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796418.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26102..26554)
                     /locus_tag="U3995_001084"
     CDS             complement(26102..26554)
                     /locus_tag="U3995_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1796419.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26689..27294)
                     /locus_tag="U3995_001085"
     CDS             complement(26689..27294)
                     /locus_tag="U3995_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1796420.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27744..28364)
                     /locus_tag="U3995_001086"
     CDS             complement(27744..28364)
                     /locus_tag="U3995_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1796421.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28366..29235)
                     /locus_tag="U3995_001087"
     CDS             complement(28366..29235)
                     /locus_tag="U3995_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1796422.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29258..29665)
                     /locus_tag="U3995_001088"
     CDS             complement(29258..29665)
                     /locus_tag="U3995_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1796423.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29684..30973)
                     /locus_tag="U3995_001089"
     CDS             complement(29684..30973)
                     /locus_tag="U3995_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1796424.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8797 a         6710 c         5126 g        10341 t
ORIGIN      
        1 cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
       61 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa
      121 aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg
      181 cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt
      241 aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt
      301 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg
      361 tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt
      421 ttggttcggt tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg
      481 aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca
      541 gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc
      601 cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca
      661 gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt
      721 aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc
      781 cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac
      841 ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct
      901 agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc
      961 agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga
     1021 atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc
     1081 agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt
     1141 ttatttttct ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt
     1201 catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag
     1261 ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca
     1321 gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac
     1381 gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta
     1441 tatcttcata aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg
     1501 caaagagctg acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa
     1561 attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca
     1621 atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt
     1681 ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc
     1741 acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg
     1801 atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca
     1861 tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt
     1921 ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa
     1981 ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct
     2041 tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac
     2101 acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg
     2161 atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata
     2221 atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt
     2281 gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct
     2341 tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct
     2401 tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa
     2461 accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg
     2521 gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt
     2581 tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt
     2641 agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta
     2701 ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta
     2761 taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga
     2821 ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat caaccctttt
     2881 ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg
     2941 ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg
     3001 tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct
     3061 tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc
     3121 tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa
     3181 ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt
     3241 ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac
     3301 ttactgatta gataccagct acactcacaa aacatacccc attcttctag gtcttattta
     3361 ttttccactt aactatagca agacacattt tggttgtcaa actttagtat gttttataga
     3421 tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga
     3481 ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat
     3541 gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta
     3601 atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa
     3661 attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg
     3721 tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg
     3781 cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc
     3841 ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg
     3901 cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc
     3961 cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc
     4021 cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca
     4081 aaactaggat cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt
     4141 atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag
     4201 tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag
     4261 gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga
     4321 gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact
     4381 cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg
     4441 cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta
     4501 ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac
     4561 ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta
     4621 ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc
     4681 caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg
     4741 tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag
     4801 cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct
     4861 tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca
     4921 gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat
     4981 agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg
     5041 taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa
     5101 tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag
     5161 gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga
     5221 ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca
     5281 ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa
     5341 cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc
     5401 catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat
     5461 ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata
     5521 cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt
     5581 cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct
     5641 catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga
     5701 ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa
     5761 taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt
     5821 cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga
     5881 ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt
     5941 gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt
     6001 actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca
     6061 aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca
     6121 gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg
     6181 atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg
     6241 ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata
     6301 tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta
     6361 ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc
     6421 ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct
     6481 tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa
     6541 ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca
     6601 attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt catgacaata
     6661 aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat
     6721 aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga
     6781 taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga
     6841 ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac
     6901 atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat
     6961 actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg
     7021 ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact
     7081 aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt
     7141 ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt
     7201 tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt
     7261 ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca
     7321 tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa
     7381 ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg
     7441 tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccatcat atgcaaacgc
     7501 tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt
     7561 ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct
     7621 tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct
     7681 gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat
     7741 tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc
     7801 agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact
     7861 actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg
     7921 taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt
     7981 aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc
     8041 cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg
     8101 gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc
     8161 cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt
     8221 gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg
     8281 taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg
     8341 atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc
     8401 aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa
     8461 gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga
     8521 atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata
     8581 tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg
     8641 tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa
     8701 acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt
     8761 tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt
     8821 ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact
     8881 tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac
     8941 gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc
     9001 agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca
     9061 atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg
     9121 ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat
     9181 tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat
     9241 ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag
     9301 cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt
     9361 caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc
     9421 ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc
     9481 gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc
     9541 atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt
     9601 tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt
     9661 agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc
     9721 attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat
     9781 ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg
     9841 gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc
     9901 tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc
     9961 cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg
    10021 aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc
    10081 tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat
    10141 tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac
    10201 ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc
    10261 aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc
    10321 tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt
    10381 ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat
    10441 acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca
    10501 ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg
    10561 tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa
    10621 aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt
    10681 atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat
    10741 tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact
    10801 gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg
    10861 taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag
    10921 attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga
    10981 ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa
    11041 aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca
    11101 catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat
    11161 aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc
    11221 ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag
    11281 tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg
    11341 ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc gttattgata
    11401 acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat atgctttttc
    11461 aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg
    11521 atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac
    11581 atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt
    11641 atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata
    11701 catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca
    11761 gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc
    11821 tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc
    11881 ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga
    11941 atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc
    12001 aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat
    12061 ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact
    12121 tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa
    12181 aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg
    12241 aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg
    12301 gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt
    12361 gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag
    12421 catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga
    12481 cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa
    12541 ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact
    12601 tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg
    12661 ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc
    12721 cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt
    12781 tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg
    12841 caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag
    12901 ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta
    12961 gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt
    13021 catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg
    13081 aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt
    13141 tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt
    13201 cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    13261 atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa
    13321 agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg
    13381 ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc
    13441 ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg
    13501 actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg
    13561 gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt
    13621 gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca
    13681 ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta
    13741 gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct
    13801 ttgatagctt ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct
    13861 tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac
    13921 agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca
    13981 atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc agttattaag
    14041 tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag
    14101 ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt
    14161 tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt
    14221 gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca
    14281 tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa
    14341 cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt
    14401 gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta
    14461 acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta
    14521 tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga
    14581 ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa
    14641 acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat
    14701 cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac
    14761 atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga
    14821 tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct
    14881 tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg
    14941 ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc ccatgcacct
    15001 tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata
    15061 tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga
    15121 tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt
    15181 tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat
    15241 cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga
    15301 tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg
    15361 ttgaaacttc cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc
    15421 cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt
    15481 cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct
    15541 tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa
    15601 ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa
    15661 ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac
    15721 aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga
    15781 ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg
    15841 ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg
    15901 gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc
    15961 aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa
    16021 cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg
    16081 caattggacc tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg
    16141 tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa
    16201 gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg
    16261 gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa
    16321 cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag
    16381 tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga
    16441 tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt
    16501 tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt
    16561 ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat
    16621 cttctagttt catttgtttg gataatacgt catacacttc atttactgca tttcctacta
    16681 cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct
    16741 tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt
    16801 aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg
    16861 aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat
    16921 aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg
    16981 aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga
    17041 aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc
    17101 tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc
    17161 ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac
    17221 taggagagat tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt
    17281 ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt
    17341 cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa
    17401 tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg
    17461 ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc
    17521 acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt
    17581 tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca
    17641 aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac
    17701 gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca
    17761 tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt
    17821 ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa
    17881 atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc aaataattta
    17941 actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt
    18001 ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg
    18061 ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta
    18121 tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt
    18181 ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt
    18241 ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa
    18301 tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc
    18361 aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg
    18421 ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag
    18481 ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt
    18541 tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga
    18601 ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg
    18661 tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta
    18721 caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa
    18781 acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg
    18841 aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt ataatattca
    18901 aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa
    18961 ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc
    19021 caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga
    19081 tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct
    19141 tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat
    19201 tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat
    19261 ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct
    19321 gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta
    19381 gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat
    19441 gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa
    19501 ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc
    19561 gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat
    19621 aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt
    19681 tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca
    19741 gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact
    19801 ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt
    19861 cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga
    19921 gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg
    19981 agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg
    20041 cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga
    20101 tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc
    20161 caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc
    20221 gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca
    20281 gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct
    20341 cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct
    20401 aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat
    20461 atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac
    20521 gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg
    20581 taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa
    20641 cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg
    20701 agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat
    20761 gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg
    20821 atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg
    20881 aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt
    20941 gtctcttcct ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat
    21001 cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg
    21061 gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg
    21121 gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg
    21181 agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc
    21241 taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa
    21301 atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat
    21361 ggataattca tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca
    21421 tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag
    21481 atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat
    21541 atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg
    21601 ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata
    21661 caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat
    21721 tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg
    21781 ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg
    21841 attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc
    21901 ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata
    21961 cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa
    22021 atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt
    22081 tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg
    22141 ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag
    22201 tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca
    22261 gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt
    22321 atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc
    22381 ttggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
    22441 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
    22501 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
    22561 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
    22621 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
    22681 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
    22741 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
    22801 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
    22861 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
    22921 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
    22981 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
    23041 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
    23101 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
    23161 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
    23221 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
    23281 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
    23341 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
    23401 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
    23461 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
    23521 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
    23581 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
    23641 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
    23701 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
    23761 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
    23821 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
    23881 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
    23941 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
    24001 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
    24061 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
    24121 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
    24181 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
    24241 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
    24301 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
    24361 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
    24421 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
    24481 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
    24541 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
    24601 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
    24661 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
    24721 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
    24781 caaacttttt ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt
    24841 tataattctg cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat
    24901 gtttgatcga ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca
    24961 gcattgatgg attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt
    25021 tgggcttcac gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt
    25081 tcttccggaa gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct
    25141 tgggcttttc ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt
    25201 acgccaaaag acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat
    25261 tcccgttctt gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta
    25321 gtcttgaatg tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt
    25381 tcttctgtaa ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt
    25441 tcttcacttt cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt
    25501 gcttcaggaa atacttccca gaaattggta tccactatta cttttttcat ctgttcacac
    25561 ctcttgatta gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat
    25621 aaaaaacatg ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat
    25681 cgttgtgttc ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa
    25741 tagctgtatc tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga
    25801 ttcgctcgat ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta
    25861 gtttcaggta acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat
    25921 cttttagctc ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat
    25981 gctccggtga attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta
    26041 gtaagtctac atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct
    26101 ttcaatagat ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc
    26161 atactttatt taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg
    26221 cgactgcatt ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt
    26281 tagtcggata gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc
    26341 cattggattc taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt
    26401 ctgctagttt ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact
    26461 gtcctcttgt taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg
    26521 caatcattcc gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta
    26581 agactagttc acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct
    26641 tttttaataa ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata
    26701 gatctggttt ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag
    26761 ctttggcaac tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa
    26821 cttggcctct tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg
    26881 tggaatatct ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt
    26941 taatcctgtt tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc
    27001 agcaaatcca ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt
    27061 gccatctttt ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat
    27121 cgttgtgagc gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa
    27181 tgaaatcgtc cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag
    27241 accagcttga gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt
    27301 cccttctttt ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac
    27361 ggcaaataaa tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa
    27421 aagacattcg acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt
    27481 aataagcccc accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat
    27541 tgaggcagga cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc
    27601 ggtcacaagt cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt
    27661 gactcggagc tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt
    27721 ttcgatatta agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc
    27781 aatagtggag tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct
    27841 aactggaatt tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata
    27901 tgattggtcc ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata
    27961 tagctcaaat gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca
    28021 aagtctgctg ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg
    28081 agtcgtgctt tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct
    28141 tccatataca aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc
    28201 gcccgttctt ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg
    28261 agcgtgatgg catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt
    28321 cctgtaccag gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa
    28381 gttaattgga ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac
    28441 tgcttcattt gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa
    28501 ggtttcttta gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg
    28561 gatcccgtta ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat
    28621 aactaggggg acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt
    28681 ttttgatttg aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt
    28741 agcttctaca aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac
    28801 atggattcct tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg
    28861 cgcatgggca tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg
    28921 ggacccatca tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa
    28981 gtctcgggcg tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt
    29041 cattacattg actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat
    29101 aatgatcggc gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt
    29161 atgcgtatta aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt
    29221 caatgttgta tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta
    29281 cttgttttgc aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat
    29341 catcaccagc acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat
    29401 acccatctcc gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat
    29461 cgttcacata caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat
    29521 gcttatcttg ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt
    29581 cggtcgcttc tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt
    29641 cttttttctt aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa
    29701 ctgggcttaa gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg
    29761 tagcattgtt gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa
    29821 taaagaaacc atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc
    29881 caaatatccc tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg
    29941 ctaagccgaa catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga
    30001 aacctaaaat gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga
    30061 tcgcatcagg aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca
    30121 gtagacgtac accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa
    30181 aggcaaacat gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa
    30241 ataaaacagt gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt
    30301 gcagaaattc gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt
    30361 tctcaccaaa aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac
    30421 ctaaagcaat atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca
    30481 tagcaatggc gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca
    30541 gaccggcaaa atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag
    30601 ttaaaaaaat gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg
    30661 ctaagaacgt cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga
    30721 tagattcaat agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga
    30781 aataagttaa acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga
    30841 gtgtctttaa cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca
    30901 acatagcaat caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat
    30961 tgataacgcc catg
//
LOCUS       DASJPH010000027        30459 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJPH010000027 DASJPH010000000
VERSION     DASJPH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.027"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(451..1761)
                     /locus_tag="U3995_001090"
     CDS             complement(451..1761)
                     /locus_tag="U3995_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1796425.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="U3995_001091"
     CDS             complement(1773..3131)
                     /locus_tag="U3995_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796426.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="U3995_001092"
     CDS             complement(3333..4901)
                     /locus_tag="U3995_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1796427.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="U3995_001093"
     CDS             complement(4905..5315)
                     /locus_tag="U3995_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1796428.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="U3995_001094"
     CDS             complement(5312..6235)
                     /locus_tag="U3995_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1796429.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="U3995_001095"
     CDS             complement(6232..7110)
                     /locus_tag="U3995_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1796430.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="U3995_001096"
     CDS             complement(7097..8434)
                     /locus_tag="U3995_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1796431.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="U3995_001097"
     CDS             complement(8437..9540)
                     /locus_tag="U3995_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1796432.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="U3995_001098"
     CDS             complement(9614..11122)
                     /locus_tag="U3995_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1796433.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="U3995_001099"
     CDS             complement(11088..11780)
                     /locus_tag="U3995_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1796434.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="U3995_001100"
     CDS             complement(11802..12776)
                     /locus_tag="U3995_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796435.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="U3995_001101"
     CDS             13076..13948
                     /locus_tag="U3995_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796436.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="U3995_001102"
     CDS             complement(14406..16604)
                     /locus_tag="U3995_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796437.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="U3995_001103"
     CDS             16836..17828
                     /locus_tag="U3995_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796438.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="U3995_001104"
     CDS             17888..19309
                     /locus_tag="U3995_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1796439.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="U3995_001105"
     CDS             19302..20801
                     /locus_tag="U3995_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1796440.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="U3995_001106"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="U3995_001106"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1796441.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="U3995_001107"
     CDS             22393..23076
                     /locus_tag="U3995_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1796442.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="U3995_001108"
     CDS             23078..23914
                     /locus_tag="U3995_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1796443.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="U3995_001109"
     CDS             23925..25679
                     /locus_tag="U3995_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796444.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="U3995_001110"
     CDS             complement(25955..26296)
                     /locus_tag="U3995_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1796445.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="U3995_001111"
     CDS             complement(26364..28535)
                     /locus_tag="U3995_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1796446.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28704..29603
                     /locus_tag="U3995_001112"
     CDS             28704..29603
                     /locus_tag="U3995_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1796447.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="U3995_001113"
     CDS             complement(29674..>30459)
                     /locus_tag="U3995_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1796448.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DASJPH010000028        28635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJPH010000028 DASJPH010000000
VERSION     DASJPH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.028"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U3995_001114"
     CDS             7..624
                     /locus_tag="U3995_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796449.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="U3995_001115"
     CDS             1644..2813
                     /locus_tag="U3995_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1796450.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="U3995_001116"
     CDS             3286..3840
                     /locus_tag="U3995_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1796451.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="U3995_001117"
     CDS             complement(4013..4486)
                     /locus_tag="U3995_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1796452.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="U3995_001118"
     CDS             4498..5034
                     /locus_tag="U3995_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1796453.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="U3995_001119"
     CDS             5074..5445
                     /locus_tag="U3995_001119"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1796454.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="U3995_001120"
     CDS             5729..7000
                     /locus_tag="U3995_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1796455.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="U3995_001121"
     CDS             7245..8270
                     /locus_tag="U3995_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796456.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="U3995_001122"
     CDS             8564..9673
                     /locus_tag="U3995_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1796457.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="U3995_001123"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="U3995_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="U3995_001124"
     CDS             10668..11492
                     /locus_tag="U3995_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796458.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="U3995_001125"
     CDS             11524..12834
                     /locus_tag="U3995_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN1796459.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="U3995_001126"
     CDS             12982..14241
                     /locus_tag="U3995_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1796460.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="U3995_001127"
     CDS             14251..15123
                     /locus_tag="U3995_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796461.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="U3995_001128"
     CDS             15135..15965
                     /locus_tag="U3995_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796462.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="U3995_001129"
     CDS             16005..18188
                     /locus_tag="U3995_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1796463.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="U3995_001130"
     CDS             18249..19469
                     /locus_tag="U3995_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN1796464.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="U3995_001131"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="U3995_001131"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN1796465.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="U3995_001132"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="U3995_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="U3995_001133"
     CDS             22431..22640
                     /locus_tag="U3995_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1796466.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="U3995_001134"
     CDS             22633..22782
                     /locus_tag="U3995_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN1796467.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="U3995_001135"
     CDS             22936..23601
                     /locus_tag="U3995_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1796468.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="U3995_001136"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="U3995_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1796469.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="U3995_001137"
     CDS             25304..25537
                     /locus_tag="U3995_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1796470.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="U3995_001138"
     CDS             25534..25941
                     /locus_tag="U3995_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1796471.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="U3995_001139"
     CDS             complement(27078..27596)
                     /locus_tag="U3995_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN1796472.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         9843 a         4313 c         5244 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc actta
//
LOCUS       DASJPH010000029        28603 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJPH010000029 DASJPH010000000
VERSION     DASJPH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.029"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1877
                     /locus_tag="U3995_001140"
     CDS             237..1877
                     /locus_tag="U3995_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1796473.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="U3995_001141"
     CDS             2031..3353
                     /locus_tag="U3995_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1796474.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="U3995_001142"
     CDS             complement(3415..3846)
                     /locus_tag="U3995_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796475.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="U3995_001143"
     CDS             complement(3910..4746)
                     /locus_tag="U3995_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN1796476.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="U3995_001144"
     CDS             complement(4936..5238)
                     /locus_tag="U3995_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1796477.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U3995_001145"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U3995_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1796478.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="U3995_001146"
     CDS             complement(5692..5958)
                     /locus_tag="U3995_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1796479.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="U3995_001147"
     CDS             complement(6212..6937)
                     /locus_tag="U3995_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1796480.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="U3995_001148"
     tRNA            complement(7303..7373)
                     /locus_tag="U3995_001148"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="U3995_001149"
     CDS             7490..8242
                     /locus_tag="U3995_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1796481.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="U3995_001150"
     CDS             8258..8737
                     /locus_tag="U3995_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1796482.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="U3995_001151"
     CDS             complement(8988..10568)
                     /locus_tag="U3995_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1796483.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="U3995_001152"
     CDS             complement(10561..11304)
                     /locus_tag="U3995_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796484.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="U3995_001153"
     CDS             complement(11480..12256)
                     /locus_tag="U3995_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796485.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="U3995_001154"
     CDS             complement(12504..13340)
                     /locus_tag="U3995_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1796486.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="U3995_001155"
     CDS             complement(13351..14280)
                     /locus_tag="U3995_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1796487.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="U3995_001156"
     CDS             14472..14825
                     /locus_tag="U3995_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1796488.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="U3995_001157"
     CDS             complement(14899..15555)
                     /locus_tag="U3995_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1796489.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="U3995_001158"
     CDS             15781..17715
                     /locus_tag="U3995_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1796490.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="U3995_001159"
     CDS             complement(17801..19024)
                     /locus_tag="U3995_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1796491.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="U3995_001160"
     CDS             complement(19018..20916)
                     /locus_tag="U3995_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1796492.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="U3995_001161"
     CDS             complement(20910..22028)
                     /locus_tag="U3995_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1796493.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="U3995_001162"
     CDS             complement(22111..22944)
                     /locus_tag="U3995_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796494.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="U3995_001163"
     CDS             complement(22937..23854)
                     /locus_tag="U3995_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796495.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23877..24056)
                     /locus_tag="U3995_001164"
     CDS             complement(23877..24056)
                     /locus_tag="U3995_001164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796496.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24081..25205)
                     /locus_tag="U3995_001165"
     CDS             complement(24081..25205)
                     /locus_tag="U3995_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1796497.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="U3995_001166"
     CDS             25388..26296
                     /locus_tag="U3995_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1796498.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="U3995_001167"
     CDS             26437..28050
                     /locus_tag="U3995_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1796499.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DASJPH010000030        28419 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJPH010000030 DASJPH010000000
VERSION     DASJPH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.030"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(414..1358)
                     /locus_tag="U3995_001168"
     CDS             complement(414..1358)
                     /locus_tag="U3995_001168"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1796500.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1654..2598)
                     /locus_tag="U3995_001169"
     CDS             complement(1654..2598)
                     /locus_tag="U3995_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796501.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2819..4033
                     /locus_tag="U3995_001170"
     CDS             2819..4033
                     /locus_tag="U3995_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796502.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4055..6070
                     /locus_tag="U3995_001171"
     CDS             4055..6070
                     /locus_tag="U3995_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN1796503.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6153..7526)
                     /locus_tag="U3995_001172"
     CDS             complement(6153..7526)
                     /locus_tag="U3995_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1796504.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7552..7836)
                     /locus_tag="U3995_001173"
     CDS             complement(7552..7836)
                     /locus_tag="U3995_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1796505.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7838..9853)
                     /locus_tag="U3995_001174"
     CDS             complement(7838..9853)
                     /locus_tag="U3995_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1796506.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10032..10337)
                     /locus_tag="U3995_001175"
     CDS             complement(10032..10337)
                     /locus_tag="U3995_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1796507.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10426..11457)
                     /locus_tag="U3995_001176"
     CDS             complement(10426..11457)
                     /locus_tag="U3995_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1796508.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11664..12539
                     /locus_tag="U3995_001177"
     CDS             11664..12539
                     /locus_tag="U3995_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1796509.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12691..13533)
                     /locus_tag="U3995_001178"
     CDS             complement(12691..13533)
                     /locus_tag="U3995_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1796510.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13625..15502)
                     /locus_tag="U3995_001179"
     CDS             complement(13625..15502)
                     /locus_tag="U3995_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1796511.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15499..16920)
                     /locus_tag="U3995_001180"
     CDS             complement(15499..16920)
                     /locus_tag="U3995_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1796512.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16923..20312)
                     /locus_tag="U3995_001181"
     CDS             complement(16923..20312)
                     /locus_tag="U3995_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1796513.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20569..21960
                     /locus_tag="U3995_001182"
     CDS             20569..21960
                     /locus_tag="U3995_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796514.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22033..22680)
                     /locus_tag="U3995_001183"
     CDS             complement(22033..22680)
                     /locus_tag="U3995_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1796515.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22688..23374)
                     /locus_tag="U3995_001184"
     CDS             complement(22688..23374)
                     /locus_tag="U3995_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796516.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23380..24453)
                     /locus_tag="U3995_001185"
     CDS             complement(23380..24453)
                     /locus_tag="U3995_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796517.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24675..25349
                     /locus_tag="U3995_001186"
     CDS             24675..25349
                     /locus_tag="U3995_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796518.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25354..26694
                     /locus_tag="U3995_001187"
     CDS             25354..26694
                     /locus_tag="U3995_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1796519.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26843..27829)
                     /locus_tag="U3995_001188"
     CDS             complement(26843..27829)
                     /locus_tag="U3995_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1796520.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8350 a         5344 c         5115 g         9610 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      421 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      481 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      541 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      601 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      661 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      721 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      781 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      841 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      901 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      961 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1021 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1081 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1141 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1201 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1261 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1321 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1381 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1441 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1501 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1561 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1621 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1681 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1741 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1801 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1861 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1921 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1981 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2041 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2101 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2161 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2221 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2281 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2341 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2401 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2461 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2521 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2581 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2641 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2701 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2761 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2821 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2881 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2941 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     3001 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     3061 tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     3121 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3181 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3241 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3301 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3361 tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa
     3421 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3481 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3541 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3601 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3661 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3721 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3781 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3841 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3901 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3961 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     4021 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     4081 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     4141 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4201 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4261 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4321 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4381 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4441 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4501 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4561 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4621 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4681 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4741 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4801 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4861 atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4921 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4981 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     5041 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     5101 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     5161 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5221 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5281 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5341 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5401 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5461 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5521 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5581 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5641 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5701 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5761 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5821 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5881 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5941 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     6001 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     6061 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     6121 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6181 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6241 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6301 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6361 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6421 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6481 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6541 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6601 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6661 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6721 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6781 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6841 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6901 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6961 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     7021 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     7081 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     7141 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7201 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7261 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7321 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7381 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7441 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7501 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7561 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7621 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7681 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7741 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7801 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7861 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7921 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7981 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     8041 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     8101 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     8161 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8221 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8281 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8341 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8401 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8461 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8521 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8581 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8641 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8701 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8761 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8821 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8881 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8941 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     9001 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     9061 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     9121 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9181 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9241 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9301 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9361 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9421 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9481 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9541 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9601 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9661 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9721 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9781 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9841 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9901 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9961 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
    10021 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
    10081 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
    10141 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10201 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10261 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10321 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10381 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10441 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10501 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10561 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10621 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10681 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10741 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10801 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10861 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10921 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10981 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    11041 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    11101 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    11161 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11221 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11281 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11341 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11401 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11461 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11521 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11581 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11641 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11701 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11761 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11821 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11881 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11941 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    12001 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    12061 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    12121 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12181 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12241 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12301 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12361 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12421 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12481 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12541 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12601 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12661 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12721 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12781 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12841 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12901 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12961 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    13021 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    13081 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    13141 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13201 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13261 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13321 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13381 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13441 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13501 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13561 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13621 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13681 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13741 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13801 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13861 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13921 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13981 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    14041 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    14101 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    14161 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    14221 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    14281 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    14341 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    14401 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14461 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14521 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14581 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14641 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14701 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14761 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14821 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14881 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14941 cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    15001 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    15061 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    15121 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15181 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15241 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15301 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15361 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15421 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15481 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15541 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15601 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15661 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15721 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15781 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15841 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15901 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15961 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    16021 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    16081 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    16141 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16201 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16261 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16321 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16381 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16441 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16501 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16561 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16621 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16681 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16741 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16801 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16861 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16921 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16981 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    17041 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    17101 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    17161 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17221 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17281 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17341 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17401 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17461 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17521 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17581 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17641 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17701 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17761 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17821 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17881 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17941 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    18001 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    18061 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    18121 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18181 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    18241 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18301 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18361 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18421 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18481 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18541 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18601 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18661 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18721 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18781 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18841 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18901 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18961 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    19021 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    19081 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    19141 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19201 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19261 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19321 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19381 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19441 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19501 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19561 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19621 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19681 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19741 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19801 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19861 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19921 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19981 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    20041 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    20101 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    20161 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20221 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20281 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20341 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20401 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20461 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20521 gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat ggaaaaaatc
    20581 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20641 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20701 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20761 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20821 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20881 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20941 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    21001 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    21061 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    21121 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21181 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21241 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21301 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21361 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21421 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21481 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21541 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21601 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21661 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21721 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21781 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21841 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21901 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21961 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    22021 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    22081 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    22141 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22201 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22261 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22321 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22381 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22441 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22501 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22561 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22621 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22681 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22741 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22801 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22861 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22921 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22981 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    23041 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    23101 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    23161 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23221 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23281 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23341 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23401 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23461 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23521 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23581 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23641 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23701 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23761 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23821 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23881 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23941 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    24001 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    24061 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    24121 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24181 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24241 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24301 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24361 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24421 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24481 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24541 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24601 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24661 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24721 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24781 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24841 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24901 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24961 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    25021 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    25081 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    25141 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25201 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25261 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25321 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25381 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25441 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25501 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25561 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25621 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25681 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25741 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25801 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25861 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25921 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25981 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    26041 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    26101 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    26161 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26221 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26281 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26341 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26401 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26461 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26521 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26581 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26641 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26701 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26761 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26821 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26881 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26941 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    27001 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    27061 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    27121 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27181 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27241 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27301 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27361 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27421 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27481 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27541 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27601 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27661 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27721 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27781 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27841 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27901 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
    27961 acatttatgt taatgaaagt catattctaa taaaatgtca tttgtttcta tatgaatttt
    28021 tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc aatccatcaa
    28081 taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc taccatagaa
    28141 taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt aagaattatc
    28201 ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg attctgaaaa
    28261 aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga acgaaccaaa
    28321 tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc aattaaaaga
    28381 tcaaaagaat gactgcacta ataaaataac taaaaatgt
//
LOCUS       DASJPH010000031        28305 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJPH010000031 DASJPH010000000
VERSION     DASJPH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.031"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..1034
                     /locus_tag="U3995_001189"
     CDS             186..1034
                     /locus_tag="U3995_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1796521.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1134..1568
                     /locus_tag="U3995_001190"
     CDS             1134..1568
                     /locus_tag="U3995_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1796522.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            1749..2159
                     /locus_tag="U3995_001191"
     CDS             1749..2159
                     /locus_tag="U3995_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1796523.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2216..2656
                     /locus_tag="U3995_001192"
     CDS             2216..2656
                     /locus_tag="U3995_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1796524.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2765..3844
                     /locus_tag="U3995_001193"
     CDS             2765..3844
                     /locus_tag="U3995_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1796525.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3970..5111)
                     /locus_tag="U3995_001194"
                     /pseudo
     CDS             complement(3970..5111)
                     /locus_tag="U3995_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            5259..6812
                     /locus_tag="U3995_001195"
                     /pseudo
     CDS             5259..6812
                     /locus_tag="U3995_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6920..8002)
                     /gene="uxuA"
                     /locus_tag="U3995_001196"
     CDS             complement(6920..8002)
                     /gene="uxuA"
                     /locus_tag="U3995_001196"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1796526.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7993..9639)
                     /locus_tag="U3995_001197"
     CDS             complement(7993..9639)
                     /locus_tag="U3995_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1796527.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            9823..10485
                     /locus_tag="U3995_001198"
     CDS             9823..10485
                     /locus_tag="U3995_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1796528.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10629..11192
                     /gene="ahpC"
                     /locus_tag="U3995_001199"
     CDS             10629..11192
                     /gene="ahpC"
                     /locus_tag="U3995_001199"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1796529.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            11202..12731
                     /gene="ahpF"
                     /locus_tag="U3995_001200"
     CDS             11202..12731
                     /gene="ahpF"
                     /locus_tag="U3995_001200"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1796530.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            12888..13238
                     /locus_tag="U3995_001201"
     CDS             12888..13238
                     /locus_tag="U3995_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1796531.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            13337..13867
                     /locus_tag="U3995_001202"
     CDS             13337..13867
                     /locus_tag="U3995_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1796532.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            14021..15172
                     /locus_tag="U3995_001203"
     CDS             14021..15172
                     /locus_tag="U3995_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1796533.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(15281..17320)
                     /locus_tag="U3995_001204"
     CDS             complement(15281..17320)
                     /locus_tag="U3995_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1796534.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17378..17935)
                     /locus_tag="U3995_001205"
                     /pseudo
     CDS             complement(17378..17935)
                     /locus_tag="U3995_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(18474..18680)
                     /locus_tag="U3995_001206"
     CDS             complement(18474..18680)
                     /locus_tag="U3995_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796535.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(18696..19310)
                     /locus_tag="U3995_001207"
     CDS             complement(18696..19310)
                     /locus_tag="U3995_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1796536.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(19457..20140)
                     /locus_tag="U3995_001208"
     CDS             complement(19457..20140)
                     /locus_tag="U3995_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1796537.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(20249..20707)
                     /locus_tag="U3995_001209"
     CDS             complement(20249..20707)
                     /locus_tag="U3995_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1796538.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(20791..22554)
                     /locus_tag="U3995_001210"
     CDS             complement(20791..22554)
                     /locus_tag="U3995_001210"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1796539.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(22775..24235)
                     /locus_tag="U3995_001211"
     CDS             complement(22775..24235)
                     /locus_tag="U3995_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796540.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(24443..25369)
                     /gene="glsA"
                     /locus_tag="U3995_001212"
     CDS             complement(24443..25369)
                     /gene="glsA"
                     /locus_tag="U3995_001212"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1796541.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(25392..26819)
                     /locus_tag="U3995_001213"
     CDS             complement(25392..26819)
                     /locus_tag="U3995_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796542.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(26850..28064)
                     /locus_tag="U3995_001214"
     CDS             complement(26850..28064)
                     /locus_tag="U3995_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1796543.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         8315 a         5352 c         5033 g         9605 t
ORIGIN      
        1 atcaatttct ccaattttct aaagtaaaag ttttttaaca gtttttaacg tagaagtata
       61 ttctagaaga atgaaacata ataatttaga ctagcctaat ttttttatta attaaagtta
      121 acttaaggtg aaaagtaagt tataataaga taagagtatt attaatgaat taaggaagtg
      181 gataaatgaa agaaacgaga caatttatat ggacatacat taagattatt tttgccctct
      241 tcatattagg agtcgctatc aatatgtttt tagggcctca tcatattgct gctggaggtg
      301 tcagcggtct aggaatatta ctggaagctg ttttaggatt tgaccgagct atagttattt
      361 tagtgttgaa tatcgttatg ttggtactag ctcttctatt tttaggtaaa aaaccctttt
      421 ttaaagtact tttcggaagc ttgttttttc ctttaatcat tgcaatagtt ccagaaacaa
      481 tgattacttc agatcggttg ttgtctgtca tttttggaag tgcgattttt gctttaggag
      541 ttgcgatttt atataaaaac aactcttcta gcggcggtac gacgatccct cccctcattt
      601 ttaaaaaata tttccacttg aatacttcga ttggcttatt ttttacagac gcattcgttg
      661 tttctcttaa tctgtttgtt tttggaatag aagagtttct ttatgctatt ttatctattg
      721 caatcacttc aattgtcatg acttatatcg aaacaggtat caatcgaaag aaatctgtta
      781 tggtcatgag tgaacattat ttggaagaca tccgccttcg cttaagtgct gagattggac
      841 gtggccttac cctgctagag gcaaaaggcg ggtataatcg aaagtcaaaa gaggttttat
      901 tgatcgttgt gactgatcat gaattttccc gaatgaaacc attgatagaa gaaatcgatc
      961 caagtgcctt tgtcattgtc agcagcgtag cagaagtaat gggacgcgga tttacttatc
     1021 atccaatcga atgagttggt aagtaaatcc gctatttttt tgaatgcgtt ttctttaact
     1081 ggtataataa aagaatctag taactgttcg actgaaaagg agaggagatc tttatgcata
     1141 aaggaacgga aatagatttt gtattaaatg atagtttgaa agcactagaa ctgtatcaaa
     1201 caatctttga tgaaatacaa ctgattgagg caacagatta ccctagaggc aataatgaag
     1261 tcatttttac cttgtatggc acgagattcc atatgctgga tgaaaacacg tctcttggtt
     1321 tgactgctcc tactgctgaa catccaaata cgatctggtt caatatcatc gtctctgata
     1381 tcgaggacac acataataaa gcattacgaa atggctgtgt agagattcaa gaaatgacag
     1441 aaatgatgga ctttggtgtg actaactcaa tgttcagaga tccttttggt tatttatgga
     1501 tgcttcatca ggtccatcgt gaagtaagtt ttgaagaaag aaaacaactt tttgagaaat
     1561 atatgtaatg tttatgatga gattagtttt tgtctctgat atttttttgt atatgtttgc
     1621 tattcgagaa agcgcgtctg tttcatgtgc tttctcttat ttatcttatg ccaatccatt
     1681 tctttgcttt ccacaattat attgtgtaaa attcaattgt ataaaatata attaaaggtg
     1741 gtaattttat gaaaaaaatg tattctacga aaatgatcaa cacaggcgga cgctctggag
     1801 aagttcataa tcccgatcgt acattcgaat tgaatattgc agctcctggt aaacgcgtcg
     1861 aaaatgcaac caaccctgaa caattgtttg cagctggttt cagtgcatgt tttaacagtg
     1921 cattagaatt gatcaaacag caaaaaaata tctctggggc ttctactgtc agtgcacaag
     1981 tttcccttta ttcagaaagt gaaacgagct ttgtcttagg tgtagagatt gaaggatcta
     2041 tcgaaggtct ttctctggaa gaaacgcaag aattattaga ggcagcgcat aaagtgtgtc
     2101 cgtattcaaa agctacagct ggaaatatag aagttactct gaaagcagtt agtgaataga
     2161 cttttttgtg taaatccgct atgatacact atagtaaaac agcggaggtg gaaaaatgga
     2221 agatctatat ttagttaatc aagtatgttt tcctttatat gccgcctcta aggaaatcat
     2281 tagacactac acttccctcc tcaagccgct gaatcttacc tatacccagt atcttgtgat
     2341 gttagtttta tgggaaaaga aaaagacttc agtgaaagag ctaggaaatg atttgtgtct
     2401 ggattctggt acgctgactc ccttactaaa aaagttagca gaaaaaaatt atattaatcg
     2461 tcagcgtgaa acgggtgatg aaagaatcgt aaatgttacg ctgactcctc aaggtgctgc
     2521 gttgcaaaaa caagcagaag gtattccaca aaagatcaag tcttatcttc cacttgaaca
     2581 ggaagaattg gatacactgc aacatctgac ccgaaaaatg ctagataatt ttcatcaaga
     2641 ccagaaaaag ggctgaaact gctagatgaa cgattttatg aggaaaacca actcataaga
     2701 taacgtcatt tagcggtttt tctttttgct ctttctcttt cttattttca tattccatca
     2761 tataatgaat aacgtgttta ttttttgtta tttggaggtt gtcgatttga ccgtttatca
     2821 gcgttttttg gcgaatgaaa aaatacgccg tctcactgtt ttattatttg tcgtttttgt
     2881 tcttttctta gttagaagta tgattacaac cattttgctc acatttatct ttacttattt
     2941 gatgattcat cttgttcaaa tcattcagcg atttgtccga atccctacag gtgttttagc
     3001 tatcatgatt tatacactga tcgtttacct tatctatctg gcattcacaa agtatatccc
     3061 gatgcttatt catcaaacat ttgatatgat ccagtccgtt attgattttt acgagcatca
     3121 gccaaaagat gcagatcctg ttttacaata tatccatact tatattgagc gaaacgattt
     3181 ttttgaacag cttcaaaatg gcgcttcgat tgcgcttggc tatttacagg atataggaaa
     3241 aatggcagtg gcgtttgcta tgtcattcat cttaagtttc ttttttatga ttgaaaagaa
     3301 aaaaacaatt gtattctcaa gattgttttt aaaaagtgaa ttttcttggt tctttcaaga
     3361 tatttactat tttgccgata agtttgtcaa tacatttggt ttggtattag aagctcaatt
     3421 tgttgtcgct cttttaaata cgttgctcac tacaatcgcc ttggctgtat ttggctttca
     3481 tcagctgctg agtttagcga tcatgatttt cattttaagt ctgatcccag tagcaggtgt
     3541 tatcatttct tgtattcctc tatctttcat agcttattcc caaggaggca tccgagatgt
     3601 tgtttatatc ctgattacga ttttgattgt ccatctaatt gagtcttatg tgctgaatcc
     3661 gaagttgatg tccagcaaaa cagaactgcc gattttttat acatttgtgg tcttacttgt
     3721 tagcgaacgg ttctttggcg tttggggctt gattgtcggc attccgatct ttaccttctt
     3781 tttagatgta ttgaaagtga aaaagatccc gaaccatctt ccaaaagaaa cagatatccc
     3841 ttaaaataaa tatgaagaaa gataaagtat gaacgacccc aaatatcaga tttcaatctg
     3901 gtattttggg gtcattttct ggatatttct ttttatttga aaaatgcgtt cacagacatt
     3961 attcaaatgt tagagagatc tgttttgttt aaatcttttt gcagaaagca ttgttgctat
     4021 acttaataga atgattccgc tccacagtat aactttgttt tgtgactctc cggattttgg
     4081 tagctgttta tttttcgttt caggacagca aaggttgtat ccttgctact ttttaaggtt
     4141 tcagctgatt gggcgctatc aaatattcgt gattttttat tcagctgctc ttcatttatt
     4201 tttagaattg ctagatttga ttttttactc tctttttttc catcctcttg aattgttttt
     4261 ttgtttattt tttctggtat tccttggtct cttgcgtccg tatctggttc tggagtcaca
     4321 tctggttttt cttctggatc tacctctggt ttttctggat tttcagtttc attatctgtg
     4381 tctggatttg tttccggtac tgtttggttt ccagaatccg tatctggttc tggagttaca
     4441 tctggttttt ctccaggggt tatttcaggt tttgatttat tgtctggctc tggttgagtt
     4501 tctgtatcgg gttcttctcc aggttctatt tccggtttct gttctccgtc tggattttct
     4561 gtaggcggta cttctggctt attttcattt tctggtttat ctgttggttc ttcaacaaga
     4621 tctcctcctg agtcgatggc tgaatcttgc tcaattgtct gccgctgact atacggcgtt
     4681 aaatgatgat cgttcggtag tccatctgat accttgacag taaaaagcgc tggttttaac
     4741 aagtcctgat ttaatgtaac ggtgaaggta aatatcgctt gcttgtgttg gatagtcaaa
     4801 gtgttctcgt catagccaat cgacaaagtg ggatcaacat tctcaaatgt aacagctgat
     4861 tgtagtatag gtgctaagtc tcccgaatag tcaatttttg ctttgatggt cgctgtttgt
     4921 ccttttctta attttgcgac tcctgaattg aaggatgaaa attcatcctg aaaggtaatt
     4981 cgattgacgt tggcccactt ggtttctgtc catttggctg tttctgtctc ctctgtgtgg
     5041 actgtctgat ttatagtaag cgataagctt aaggctgcaa atgttgatat taagatggtt
     5101 gaatatctca taactctctc actccttgat ttttttgtat gcaagaaata agatagtaga
     5161 gattctacct atatgtttac tgtttttttg tctaaattta tgaaaaaaac agtaatattt
     5221 tcaagtttta acaatttaag taaaaaggag cctctttcat gcaaactcaa gaagcgatta
     5281 aaacgtttat cttgaagaaa aaaagcaata caaaattaga tatttttctt ttcctatctg
     5341 aacatcgttt ctttattact acacaatatt tagcagatca tttccatatg tctgaatcaa
     5401 attttttact atacatcaaa gaattagaac aagactttga gcgtctcaat ctaacagaac
     5461 tgcatattga taagcaaaaa ccttttttaa agttgaattt tgaaggaatt gatccggctt
     5521 actgctatta tcgtctattt ggaagatact gcaacgaatc agtcagttat cagatcttga
     5581 cttctttgtt cagctgtcag acaaattcga ttatcagttt cagtcagcaa acaaactata
     5641 gtgcctctta tttgtatacg aaaatgaaaa agattaatgc atttttagca tagtacggtt
     5701 tgtctatctc cttttctaat aatggcagaa agtctttttc tggaaaagag gtacaaattc
     5761 aatatgcagc attagatatt tattggaaca tcttttcaag tactgctaca gaattttatg
     5821 atgaagatcc gcaagtagtg gcttttatga tgaatacttt ttttaaaaaa gaagtacttg
     5881 accgactaaa tagcggtatg atagataaat tatattttct attaaaaata tgtaaagaga
     5941 attttcctgc aacttctgtg aaagacgtac aaaaagaatt cacagagtat gaatatattg
     6001 acttttttat tgatctctct gtagatattt tgcgacctaa tctgccaatt tgtcgcgaac
     6061 aacgcatctt agtgaatatt ttagctcgac tttctatcac gaaaatcgaa tcggaagaaa
     6121 caagcttgga gcaatacaaa cttttaaaaa aagcaaacgt tcctcatgtt ctttattcaa
     6181 gagaaatggt ggagtccctt tgtactgtat ttgatttgtt tatacctgaa aaagaaaaga
     6241 ttctttactc attggcattc gcacgaaata aactttataa tggatttctt aacttcaatc
     6301 aaccaaatac gccccttcct actttcatgc tttatcaaga acatagcgta ttgaaagaag
     6361 tccaaaccgc cattgaacaa ttttatatgg atttcagaga acaaaatcaa cacttgttac
     6421 cagttatttt agaaaaggaa aatgtgcaat ggatggttga agacctagta catttgtatg
     6481 atcgctacaa aaaacagccg cgaattgtga ttggtgtgaa ctatacgcgt gatttttata
     6541 tcagtaaaga tctgatcgtt aagatagaac aaattttttc aaatgaatcg atcaaaatac
     6601 aaaatgattt tatggagaac tgcaacatcg ttatcagcga ctgccctttg aaacaattgc
     6661 cttctcatat caaaaaaatc tatattttaa aaggaatcat cacaccagaa gactggaaaa
     6721 aagtgattat gaggatctca gaatatatct ttgaattaaa agaagaagca ctcatggatc
     6781 aatcgaaaaa agaaaatcaa gttacttcct agaccaaata agatacataa atccgaatgc
     6841 ttattaacaa cctccggatt tatgtatcaa gaatttgaac atccttctta tttacataat
     6901 aaaattacag tttttttcac taattatctt tacttttgtt tcctttttcg attgcttccc
     6961 ataatccatt tagataaaca gcacctaatg ctcgatcata taagccgtat cctggtcttc
     7021 ctgtctcatt ccagatcatt cttccatgat caggacggat gtatccgtta aatccgttgt
     7081 catacattgt ttttagtatg cggtacatgt ctaatgaacc gtattcggat ttatgtggtg
     7141 cctcatagaa atctcgatta gctaaaaatt taatatttcg aacatgtgca aatgggattc
     7201 gatctctttg gcaaaattct gctaaaatcc catatacatc attttctgga tcttctgcaa
     7261 tactccctgt acaaagagtg attccgttat aagggctgtc tactatgcga cacaaccaat
     7321 ctaaatcttc tcgattcttt acgattcgag ctaatccaaa aatcgagtat ggcggatcat
     7381 ctggatgtac agccattttg acccctacct cttcacatgt tgggatgatt gattggagaa
     7441 aataggcaaa attttctcga agtttttctt cgtctactga tgcataggct tcaagcaagg
     7501 atttgatagg agctaagcgc tcaggttccc atcccggcaa tccaaattca ttgctggaat
     7561 gttcgatgcg cgcgacgatt tctcttggat catctggaat atcatcagat ataaaagcta
     7621 acgtggatga gccgtcttct aagcggtagt caaggtcaga tttcacccag tcaaatacag
     7681 gcataaaatt atagcagatg acttctattc catattctgc caagtttcgg attgtctgct
     7741 tatagttttc gatatactgc tcacgggaag gtaatccaat tttaatggca tcatgcacat
     7801 tgacgctttc aattactttt agtgttaacc cacgagactc aatttcttct tttagttctt
     7861 tgatcctctc cctaggccat acctctccta ctggtatatc catcaatgtg cctacaatcc
     7921 ctttgaccgc aggaatctgt ctgatttcat ctaaggtaat cttttcttcc tgtgatccgt
     7981 accatctaaa agtcatttcc atgataaatt tcctccatgt tcttgtaagt aatcctgtaa
     8041 tgtttttcgt acggcatttg gtcctgctac catcttgatg aacatttctt ctatttttct
     8101 gccaagctct gcttggtaaa gatctacacc aaaaatttct ttatttgaca aaataggagc
     8161 cattttctca tgaatatgtg atgtatcttg cgtacccagt tgaatatcgg aaagcttatg
     8221 ctgaaggtct gtcaatagtg ggtctggact cgggatgaat ggttttcctt gatcatcaat
     8281 gccaataagg tatcgaagcc aagcagcgag agtcaatggt atgaatacaa gtgtctgcgg
     8341 gtctttaact tctatttcca tatatttttt aatcgtctca ccataacgaa tcgccacctt
     8401 ttgagaggta tccgtggcga ttcgctgagg catattagga atatttggat tagaaaagcg
     8461 cacagtaatt acttcttcca gaaagatctt tggatcaatg atacctggag actccacaac
     8521 tgggaggcct tcattgtacc cgatttcaga aactagcagc gataaatctg gatctttcat
     8581 ctcctcagct atgctcctat accccagtaa acaacccata atagctaaag acgtatgcaa
     8641 aggattaagg caagtcgtca ccttcatcgt atctgcttta tctaccgttg cacgatcagt
     8701 caagaatacc ccagcttttt cgaatttcgg acgtccattg ggaaaatgat cttccatgat
     8761 taaataatgg atgtcctctg tattgacaaa tggagcaata ttatttcctt ttgcggtttg
     8821 gaaaatcgac atatttgaaa agccctcttt tgccaacatt ttgctaactg acaaggaagg
     8881 attcggggtg atacgatcaa tcatcgtcca tggaaacgca acaatttctt cattcgataa
     8941 gtattggata aatgtatttt gtacataacc ttccttttgc cattcttttg cgatcgttag
     9001 caaagaagta cgtaattgac taccgttttg tgaaaaatta tctgtagaga caagtgcaat
     9061 cggccaagct ccagataaaa aacgtttata aagtagcaca gcaagaatac tcattgtatg
     9121 tgatggcttc tttggaccat tttttatatc gactgcaatc ggtcctatat atttattatt
     9181 tgtatctttc aatcgataac ctttttcagt aatagcaaaa gtaactaatt gtaaactagg
     9241 attttcaaag tgtcgaatca tcttatcaaa gtgttcagaa caatctggat gacaatacag
     9301 actgtcagct gtcgcagcta atatacgttt gacgaactct ccactgcttg gcatgaccac
     9361 ttcaagaatc aaatcatcaa aatcttggta agccctttca attatttctt catcgaatgt
     9421 ttcacaaaca atgacaccag tggacaattc gcctagatcc gctaaacgtt gagcgatttc
     9481 tgcatgaaat cctcgataaa gatttccgcc accgaaatgg atccagacag gatttttttg
     9541 tccattttct cttaatgatg cacggtcaaa atgaggtatt tctacttctt tttgagataa
     9601 ttcagggata aagttcctcc agttgtttaa atcaatcatt tttcccctcc taaactaaca
     9661 tattagtttt tgtattcaag tgaattttat gcaataaaaa tcgctatgtc aaccaaaatt
     9721 ttactattta tacaaattta ctagttaatg tgacatacca gattgttttt cagacctctt
     9781 ctgttatact acgttcatag aaatgatttt ggaggaataa agatgaaaag cagcttgcaa
     9841 tcacaagctt atcaagcgat tcgtaaacaa attatctatg ctgacttaga acctggaagg
     9901 aaaatatctg aaaaggcact ggaggaacat cttgctatcg gccggactcc cattcgcgaa
     9961 gcattgattc aactacgtca gcaagaactc gtgtatacga tacctcaaag tggtacgtat
    10021 gtttccaaaa ttaatcttgt ttctgcagaa aatgctcgct ttgtccgaga acagttggaa
    10081 gagcaaatca tgattgaatg ttgttccaaa ttggacacac aaacacgaat agtgctagaa
    10141 acgattttag aagaacaaga aaaagcagtc aaaataaatg acgaacgcgc ttttttccgt
    10201 gcagataatt tatttcatga agcttgtttc gaaattgcag gaagaaaaga agtttggaac
    10261 tggttagatg accataatac ccatttagaa cgtttccgct ggttacgagt gaccattcag
    10321 gggcttccat gggaaacgat catggaacaa cattatgatt tactcaaggc tttagttgat
    10381 aaaaaaccag aagaagctcg atttcaaacg ggattgcact tacacatgat gctgaaagaa
    10441 caggaaactg ttactcaacg ttatcccgag tatttccagc agtagcattt tcttgcgacc
    10501 tcttctattt ttataattaa ccttcgtttt tgagtaatct ttattaaata acaaaatatc
    10561 cattgacttt gcaactccaa ctaaattaca ataattataa ataaataatt attattggag
    10621 gttttactat gtcattgatt gggaaaaaaa tcgaagaatt taatgcacat gcttatcatc
    10681 aaggagaatt tttagaagtc agtgaaaaag atcttatggg gaactggagt attctttgct
    10741 tctatccagc tgattttact tttgtttgtc ctactgaatt agaagactta caagaacaat
    10801 acagtacatt gaaatcttta ggtgttgaag tcttttcttg ctctaccgat acccacttta
    10861 ctcacaaagc atggcatgat acatcagatg ctatcggtaa aatcgaatac gtgatgatcg
    10921 gtgatccgtc tcatcagatt tctcgtattt ttgatgtttt ggatgaagag caaggattag
    10981 ctcagcgagg aacatttatt attgatccag acggaatcgt tcaagctatg gaaatcaatg
    11041 cagatggaat cggtcgtgac gctagtgcac tgattgataa gattcgcgct gctcaatatg
    11101 tacgtaccca cccaggtgaa gtttgtccgg caaaatggaa agaaagtggc gaaacattga
    11161 aaccaagttt tgatcttgtt ggaaagattt aggtggtgga tatgttagac aaagaaacta
    11221 aacaaaatct cgaacaatat ttagcactga ttgaatctcc tattgttttt tctgttagtt
    11281 tggataatag tgaaaatagt caaaaactag ctgaatttac gaaggaaata gcagaaatgt
    11341 ctcctaaaat ctcttgggaa aaaagagatt cgatgcgcac acctagtttt tctatcaatc
    11401 cagttggcaa agaaagtgga atcgtttttg ctggtatccc cctcggtcat gaattttcct
    11461 cttttttact ggctatgtta caaattagtg gacgggcacc gaaaatcagt gaatctttga
    11521 gtcttaaaat caaaaaaata acacaaacat tgcattttga cagttatgtt agcttgactt
    11581 gtcataattg ccccgacgtt gtacaggcat tgaatatatt agctgtatta aatccaaata
    11641 ttactcatac gatgattgaa ggtgggatgt tccaaaaaga aatcgatgat aaaaaaatca
    11701 tggcagtgcc aactgtgttt ttaaatggag aagaatttag tagcggacgc atgacgatcg
    11761 aacaaattgt tgaaaaaatc actggccctt tgattgaaga agagttgttt gaaaaagaac
    11821 cttatgatgt attagtgatt ggcggtggac cagcagctag tagtgcagct atctatgcag
    11881 cacgtaaagg aatccgtaca ggacttgtcg ctgattcttt cggcggacaa gtcgttgaaa
    11941 cactagggat tgaaaatatg attggtacct cttacacaga aggtccaaaa ttgatgcagc
    12001 aagtagaaag tcatgtgcgt agttacccta tcgacattat gatgaatcaa caggccgttt
    12061 ccttaaataa agagcaacat tttattaata ttggcttagc taacggtata tcacttaaag
    12121 caaaaaccgc catcatcgcg acaggtgcgc actggcgttc tatcaatgta ccaggcgaaa
    12181 aagagtttaa aaataaagga attgcttatt gcccacactg tgacggccct ttatttaaag
    12241 gaaaagagat tgctgttatc ggtggtggta attctggtgt tgaagcagca atcgatttgg
    12301 ctgggttagc aaaacatgta tatgttctag aattcttacc agaattaaaa gcagatcaaa
    12361 tcttacagga taaactagtc tctcttgcga atgtcactgt tatcaaacat gcagcgacga
    12421 aagaaatcag cggtacgaat caggtagaat ctctctccta tatcgatcgc cgtacgatgg
    12481 agatgcatac actagctgtt tcaggagtat tcattctagt cggcttactc cctaatacgg
    12541 attggctaga tagaacgatc gaaatgaatc caagaaatga aatagtgacc gagaaaaatg
    12601 gcgcaacgaa tatccccggc gttttcgcag ctggtgattg tacagatagt ccatacaaac
    12661 aaatcattgt ttcaatgggt tcaggagcta ctgcagcttt aggagcattc gattatctga
    12721 tgcgcacttg agctatgaag attgtaaaaa gtcagagagc ggaaaaaagg atggtgacaa
    12781 gaattttgtc acaatccttt ttgttttttt gaataatcgc tttttcttct ctttttctta
    12841 tatactataa gtagtaaata atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc
    12901 gtatttttac gacaagtttt gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa
    12961 aagatcgtat aaaagaggaa gtagatgaaa tcatttgttg gctgacgggt tattcccaaa
    13021 cagaactaga atatcaacta caaaaacaag tgactttcga acaatttttt gcggaagccc
    13081 ctgtccccaa tccttcacgt tccttgatta caggtgttgt ttgtggtgta cgagtagaag
    13141 acatcgagga taacttgatg aaagaaattc gttatttaga taaaatgatc gatgagctag
    13201 caaaaggcaa aaaaatggaa aagatcctca gaagttgatt tttccagaga tattcttctc
    13261 cttcgctcat tttgtaacat ttaatttggt aaaatgacta gttcacgtta aaataagtca
    13321 aaggaggttt tcttatatgt ttgagacgat taaacaaaat attcaaaaat acgccttatt
    13381 aagagctgct gtttatatta tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt
    13441 tcattttatc ggttatctga tcaccgccta tcttgttttg cttgggttgt tgaatctttt
    13501 tgaggcttac cgttttaaaa ggaaaacagg ttcttggagt atcggtctgt tcagtggtct
    13561 ttttttcctg atcttagctc tgatcgtcta tgcttttgca cctgcaatcg tttctatcct
    13621 gccgattctt cttggattag ctgttatcat caacagcttg ttccagttgt tcttcagcat
    13681 gaatacaaaa gctaaaagtt ggagtgttta tagtagtatt ctgttgttga ttggcggatt
    13741 cgtcttattg ttcaatccat tcaaaagttt gatgcttcta ttccaaatat tcggctttat
    13801 tttgattttc atgggcatcc aggagattat caatttcatc agaataagaa aaatgactat
    13861 acgttaatca tttttagtta ttaaaataga ccgtataaaa accaacaatc caaaaaaatc
    13921 ttttggattg ttggtttttt tgtgagtata ttttcctatt ttttcaataa tgtatgttat
    13981 gctaagttcg ggctatattt tatacaaagg aatgatgaga atggaattta ttggtatttt
    14041 atgcctgata ttgatttcaa caacgattgg ctcacatatc tcgcggagat taggtatccc
    14101 agcagtgatt ggacaattac ttgttggtgt tttgctaggc caagctggat tgaattgggt
    14161 caaacctgat attctagtcc atgacttttc ggagattggt gtgatcttat taatgttttt
    14221 agctggtctg gaaagtgatt tatctctttt gaaaaaatat ttccgacctg gtatgttcgt
    14281 tgctttgctt gggatcatct tcccggttgt ttttggctgg ttgacaggca aagcattcca
    14341 agtttcggga aatgaagcga ttttctttgg gattattctt gcggcaactt ctgtcagtat
    14401 ttcagtagaa gtattaaaag aactaaacgt agtgaataca aaagaaggat caacaatttt
    14461 gggtgcttct gtggtagatg acattttagt cgttttagtt ctaagtttca gcctatcttt
    14521 tctaacgggg gaagcttcta gcaatgcttc tttaccgctt gttttgattg aacagctaat
    14581 ttattttgtt tttattttct tattagtcaa atggattgcg cctttcctac tttcatttgc
    14641 agaaaaagtg tacgcaaatt cttctattat catcgtatcg ctagttattt gtttaggaat
    14701 gtcttacgta gcggatcttg ttgggcttag ctctgtcatt ggtgcttttt ttgctgggat
    14761 agctgtcagt cagacaaagg ttaaagaaga agtataccac aatgtagagg ctttaggata
    14821 tgcagtattt ataccagtat tttttgtcag tgtaggatta gaagttgatt tttctgactt
    14881 tagtgaacaa ctactcttta tttctatatt gacgcttgta gcgattttga ccaaattgct
    14941 tggaggttat tttggtgctc agattgccgg tttttctcaa catagttcat tgatggtcgg
    15001 tgctggtatg atttcgcgtg gagaaatggc tttgatcatt ttacaaattg gacagcaaag
    15061 tcaattgatc gaaccacagt actattcacc tttagttatc gtgattttat taagtacact
    15121 tatttctcca cttgttctca aatattttac aaaaaaagtt tatgaagtgt gagtaaatca
    15181 aaaagaagtt gtgaaagaga catcgtgaaa accgtttatt tgaaaaaagg actgtgacga
    15241 taacgccaca gtccttttac tgtacttaaa aagcaatttt ttattgataa ttttggttga
    15301 ggtattgcaa tagttccgat gctcgtttgg ttgctgaatc atcatcttct ttattttcca
    15361 acatgctgac catcatataa ccttggttat caagattgaa agcaaacaag aaactgttct
    15421 cttgcccctt tacatcctgt ttttctttga tttccgctgt accagttttc gctgccaacg
    15481 gaatccctaa agcagaaaga gaatgtgctg taccatttac atcttgcaca acttctctca
    15541 gatctggcac gatcgtttgt actgctgttt cgctgatgac attcttctta tcttttgtct
    15601 ctttatctgc aatcaattta ggatagacaa gtgtgccatt gttgataaaa acagaataca
    15661 ttgctgcttg ctggatagga gtaattagaa gttcgccctg tccatatcca gtatcagcta
    15721 gcaagatatc tgagttaaag ctatcttcat tagaaatttg tgctggattc atactaattg
    15781 gcaaatcaag gtcttcacca aaaatgaatt tatctaagcc tgtacgaaat tttttctcac
    15841 ccattttcaa cgtttcttgt gccgcatata tattatcgga atagatcaaa gcagttttta
    15901 agtctacttg tgatacatca ctactgacac gcgttgtttt aaaagatccc caagaactgt
    15961 ctttttgcca tttaagcccg ttgatcgtca acacttcatt tggatcgata gtgccgttgt
    16021 cgagaccgat tgctgctgta atcattttaa acgtagatcc aggagcataa cctgtcgcaa
    16081 atcggctgat gaatggatgt tcaggatttt cttcataagc tttgtaatct tcttgtgaga
    16141 tcccgtttgt cattttgttt ggatcatagc ttggagagct agcaagcgca agaagatcac
    16201 cggtttttgg cgttgtcgca acagttgatg cagcttttcc tcctagactg tcaaaagcta
    16261 ttttttgtgc cttcgcatcg attgtcaatt tgatatcttt gccattttga acttcatgtt
    16321 cgatcagaac ttttttctct acaccgtctg catctgtaat gcttaatttc ccacctgttg
    16381 tcccacgaag atccttatca aaagccatct ccaaacctga gcgaccgatt tttccattac
    16441 tgcttaattc tggattctta tcaatatctt ctgctgtgat atctcccacg taaccaatca
    16501 gctgagcagc tgcttcaccc aaaggataat atctgccgtc tacttcttga atggtagctc
    16561 cagctggaag ttctggcgtt gctccatcaa tgattttcaa tgggacaaag taatcgggtt
    16621 gtacccagct ttgtgaaatc gcctgattga tagcatcttc tgttaagtcg aaggaggaag
    16681 caatcgcttt gatattggct gttttttcgc ctccatctcc aagtttactt ggaacgactc
    16741 ctaattgttt tagtttgcct gttgttgcta atggttcacc atttcgatct ataatattcc
    16801 ctcttgctgc ttcttgtgtg gtcaggctta ctttgtcatt tccttccatt tctggaaaaa
    16861 ccaagttggg ttgccagttg atcgtggttt gaccatcatt tctgtctaat gttcctttat
    16921 aagaaagatc tttcaattca cctaatgagg tattcatctt tgctttatat gaaaaactgt
    16981 aagtagaatc gtctttttta tctactttta gattcgatat ctgaagtcct ttgacatcag
    17041 cagcaccgta tatattctgg tatttatcta agatctcttt ttcagataat gcacttttat
    17101 ttgccgcctt ttttgatgtc atttctgcag ctttgttata atctcccttg ttcaaagctt
    17161 ggacaaattg ctcaactgtc ttttctccag cttctactgc ttgggtttct tgatagtgct
    17221 ggtagtaaaa ataaccgcca cccgcagctg ctattaagat cactgcgccg gcaatataag
    17281 cgcctgttcg atttttgccg tgcttgtcac ttcttttcat tttttcctcc ttctcttgct
    17341 tgtcattcct ttatttcact atttatacct gtttattcta acatatgtct ttgaggaaat
    17401 ccataccaca atccagaagc acactttctg gaaggttcgt ttggatacta ccaagtaatt
    17461 tcccaaccgc tttgggtagc ttgaatattg aagttacgtc tttcagctgc tgagagatag
    17521 ctttgatcac ttgctgctgc cttctgatcc tcccgaagtc tccttctttg tcttcccgaa
    17581 atcgagcata ctggagtaaa gtattaccat ccatcacttg ctctcctttg tttatatata
    17641 cgccatctaa atctagagat ttttctgcat cgatcttgac tccaccagga aacagttcat
    17701 taacgcaatc gataaaatct tgaaatgtga tacttgcata atagggagct tcaaatttga
    17761 aattttcctt taatgtttga tttaataaat caactccccc ataagagtaa gctgcattta
    17821 tcttattcat tccgtaacct ggaatatcga catatgaatc tctcatgata gagatcagtt
    17881 ttgcttgttg tgttttctgg ttgtattgtg caaccagaag tgaatccgag cgacctccac
    17941 caatcgatct tttttcaccg catctcctcc tcacacagca atccacttgt caggtgagga
    18001 gcgagtttgt ctcatcaaat aagtcagtca caaaaatata aagaaaaggt cagaaacctt
    18061 ttcgaacaag aattctgacc tagcatcatc tcatcgtccg agttttagca ctatacaacg
    18121 taaagaaaac attcttagct attggaaaaa gccttaatcc aacagtttct gttttaccca
    18181 ttggcgactt tcgacgtttc tgggcaacag cctatgtttg tataggagcc tcacctaaca
    18241 aggttattac tatattctat ttacgagttc atgatagact tttcctaatg attaggcaat
    18301 ggaatttgaa atgaaatagt gaaatttaac aatctttaat attttttaga aaaaaataac
    18361 aaataagttg aaagaatccc gttgcaaatt ctttcagttg caccaggatt ctttgataat
    18421 tttttagttt tatgtgtttt tttatatgtt ttttcacttt tcggaataaa tatttatttg
    18481 ttaaagtaat taaacaatcc gctaatcgtt tgatctggca tttctatttc cttaccatct
    18541 aaagtaacag tagtaacgac aaaatggtct tgcaatggcg gaacttctaa gtactgccct
    18601 tgcagaaaat cattcactgt ttcatattct tttttcattt cttttgaatc tgtcgatggt
    18661 ttatagctta tttttaacat aactagtgct cctttttatt cgggacgtgc aatcgtgaaa
    18721 atttctccta ttgctgcata atcatttcct gccaaacggc ttacagcatt caattttctt
    18781 ggatcgatcc ttcctgtctt atcatcatag actgtttcat ttaaatgata tttctgtact
    18841 tctagcaaaa ataaatcagc tgttacttta tttttattcc gtatctcgac atgttcatat
    18901 aaggatgttt caaaacgaac ttttgcttct gtcaatgctg gtactgaaat gattactgaa
    18961 tcagttaaag tcaagtcagt caggtacagt tcgctttcct cattaggcag attggctgct
    19021 gttttattta cttcttctac gttgtcctca tcaaggatat ggataacagc ttctcttgtc
    19081 tcaagaatat tctttgctgt gtcttttact gcatcttgtt ttctctggat cgccaaggat
    19141 aagatgggtc gattcgaact gacgatattg aagtaactga agggtgcgat attcactata
    19201 tcttttgctg atttagtcgt tactactgct actggtctag ggatgactga accaatcagc
    19261 aacttgtaat tttcacgttc tgtcaattct gaaggactcc gttcaaacat ggtttcgcct
    19321 tctttctata aaattcgtta aaaagcagat cttgaagaca aaatgaaaag taatgctttt
    19381 ttcatcttct tagaatctgc tttaaactat ctatcaattt ttcttttttg tcaagaatct
    19441 gtgccgtgcg tgttttttag ttatttgctt tacttgcggc ttcgacatat cttttagtga
    19501 tcaattgcgg acgggtgata atagatccaa ctacgactga aaaagcacct aattcatatg
    19561 ctttctctac ttgttcagga gagtcagtat gtccttccat aattactggg atatcgactt
    19621 gttctatgat ttgattcaat aaatcaaaat caggtgtctg cgtctttcgt gaatattctg
    19681 tatagcctgc taaagtcgta gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt
    19741 cctcaaaagt cgaaatatct gccatagcta accgaccgtg ttcgtgaatc atttggacaa
    19801 gttcagcagt tgttttttct gtcggacgtt tacgagaagt tgcatccagt gcaatgattt
    19861 ctgcttgcgt ctccaaaagt tcttccactt ctttttctgt cgcagtaata aacacttctg
    19921 acccatcgta atctcttttg ataatcccta ttaccggtaa atcaacgatt cgcttgattt
    19981 cattgatatc ttctgtcgta tttgctcgaa taccaactgc tccgccttct tttgcagcca
    20041 aagccattct ccccataata aaagaactgt gtagcggctc gttttccaaa gcttgacaag
    20101 aaacgacgac tccgcctttg atttgttcca gtgtatccac aatatctcct ccagtaatgt
    20161 tatataaaaa tacagcttca tataaaaatg taaccctttt ctattcagga gacaataatg
    20221 agctatttct ttcagcagac ctttcttact aaaagaaaat tacttcaaat tcttcgtata
    20281 cgaccagctt ctcttctgta aattctatgt tcaacgcttc tttataagca tgtttaaaaa
    20341 actcagcatg gactttccgc caataagcta atgtccgatc cccttctcct tctttccaag
    20401 catgctcttt cgtgacttca ctaaaaggca agatggtcac atttatattt ttgatgatac
    20461 aaactggttc attttttcca ttcaggacca cgtctaattg accaactttt ggtaaaggct
    20521 cattctcttg tttatacaga tcgtaaccac ttgatgtacc agtctttatc ttttttacaa
    20581 ctagttcgct caactcgtct gccatttttt ctgtatcccc aaatgaaaag aaacctaatt
    20641 tttctgattg ataactagga tatttttctt ggaacttttt gacatattct tctacttgct
    20701 gatacataac atccacccct ttgcttttta taatgaaaaa accatccaga tactagtata
    20761 aacaattatc tggatggtga gcataatgaa ttattgaata ttcaagacac gcagactatc
    20821 ttcaacgatt tgttttggtg ttaaatgata acgttcataa agctgcccta caggaacgct
    20881 atccgtgaat tctttctctg caccaaaatt taatacgttc attgaagaat caccataata
    20941 acgactgata ttttcgccaa atcctcctga caaactacca tcttctaatg tgatgactaa
    21001 ttcgtgtgtt tcttttaatt tatctaatgt ttctacgtca ttttctgtga tcgttcctgg
    21061 attgatcaac gttgcttcga ttcctttttt tctaaaagct tttactgttt cttttcctaa
    21121 ttcaagaaaa ccgcctaaag ctaaaattgc tacttttcct ccattattta ctgtttgata
    21181 ggctggaata ctataatcat ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat
    21241 tcggattgct acaggctgcg ctgtttgttc gaacgcccaa tctagcatag agatcatatc
    21301 ctcttttgtt gctggagcaa gatatttgat gttcgggata cttgataagt aaggaatgtc
    21361 aaatgttcct tggtgtgttc ggtcactagc tgagatcgtt ccgccaccga ctaaaataat
    21421 cactggattc tcgttgatcg ctaaatcatg tgataattga tcatatgccc gttgcaagaa
    21481 agtacttgat tggaagacga ctggacgtac tccctgagaa gccaaagcag aagcgaaagt
    21541 gatagagtgc tgttcagcga tccccacgtc aaaataatga tctggatgtt tttcttggaa
    21601 tgttttcagt ccaaaggcgc ctggtatcgc tgcattgact gcggcaatag gagctgtact
    21661 tgtttgcatt ttcttatcca aataatccat gatgatcata ttaaatgttt ctccagaagt
    21721 tacttgcttt gcctccccgg tggacagatc aaatggtaca tgccaatgat atttcatttt
    21781 attatcgatt gccggctgat aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg
    21841 atgatcaata tcttttactt cttcaaataa acggatcacc gtttccagat cgtttccatt
    21901 atccacgtaa cgataatcaa atcccattgc tttaaatagg ttattgctgc ttttaccgtt
    21961 ggtttgacga agctcagcta agtttttata taaactgcca tgattctcat caatagacat
    22021 ctcattatcg tttactaata cgattagatt ggattgtaaa acaccaccat tgttcaatcc
    22081 ttcaaaagct aagccaccac ttagggagcc gtcaccgata aatgctacca catttcctga
    22141 ttctttcttc agatctctcg ctttagctaa acctgttgct aagctgatag atgtagatgt
    22201 gtgccccact gtaaaatagt catgtttact ttcatgttgg ttggaatatc cgctcacttc
    22261 acggtaatgt cctttttcaa aaccattttt tcgaccagtt aaaagcttat gaccataaga
    22321 ctgatgggaa acatcccaaa cgattttatc ctttggtgaa tcaaatacat aatgaaacgc
    22381 aatcgttaat tctactacac ctagattcgg accaacgtgc ccacctacat cgctgtcctt
    22441 ttgcaagatt ttttggcgca tctcttctgc caactgtttc atttcctcaa cagataattt
    22501 tttgacatct gcggggctat tgattgcatc caatacatgt aatactgtac tcataataca
    22561 ttctctcctc cgtactatct tcttttaatg aaaatagatt tgttacactt tatctattct
    22621 actacttaga gttgactcta agtcaatagc attttaaaaa aatttcagag aaagcttgtt
    22681 atataagtag atcttcgcac gaatcaggac tcaaataaaa accttctaca gacaattaaa
    22741 aagctttgta gaaggtctcc taattaaggt ctcatcaaga aatatgtttt tttaataggt
    22801 gaagttcctg agcaatatga tacaaatgga tgtttgcacc ttttaatgag attggataga
    22861 tctttgtctt ttcagtttct aatccatttt gatagctagc agcatcgcac aaaaccactc
    22921 tttttttcgc acatagacat tcatcaggca attcttctaa tgaacgaaat aaaataggat
    22981 ttatttcagc gatgccttct tccgcataca aatgaaattg ggttcgataa aaaaattgtt
    23041 tgactgaact ttctgaaaca acaaaaattt ctatttcctg tctccccgct tgttcactgt
    23101 tgatcaaaga aagacttttg acgaattttc gaatgaaatt ttcattccat cttagttgga
    23161 tttgattttt ctttttccac tcttccaata tttctacaat ttttccgtac agaagttgtt
    23221 ctttttctga tagaagatag atcctttccg gtaaaaatac ttgtccatct gcccacaacg
    23281 tacggacaaa ttgtaaaaag gattgcttga aaataaaatt ttctgcatcg tcgatttcag
    23341 cgatactaat aatctgtaaa atcaattcat tcagtgatag gtcattgaga acaaaatttt
    23401 tataaacaac ttcgaaatct tttcttaaca gctccatgtt ctcattggta taatttctac
    23461 tgttgaacaa agaaaaaata taatagacat cttcttcttg taagtctaga attccttttt
    23521 gctcttccaa tccttgacag actaattgaa ataacggctt cgtttctgcg cttttcttat
    23581 cttcttcttt cagtccaagg ggaaatgcct ttcttccgat tgcgatctct attcctcgta
    23641 tgataaagga cttttcatct tccgaatatc ttcttttaaa aaaatgagtc tctacataat
    23701 caatcaactc gttacaagct tcttctacat acggattttt attttcatag ccttcccaat
    23761 tgacataacg tactaaagct agcaagatgt tccgcgagtc aaattctggg ataatgattt
    23821 ctttattttt caaataatca ttttctttaa aaaaatccaa tacgattttt ttgattgtat
    23881 atactttgct caaagaaata aattcaagat cagccagttc tgtactcgtg aatataccat
    23941 taaaataata acttaaaaac tttaaaaaat aagattgacc gtacagaaac tgtgtgagct
    24001 cagaaagagc aataccatgt ttgaatacga gtttatactt atgcttctct cttacttgtt
    24061 cttttataca atattctaga cactcggcta gactttctag atcgtgaagg atcgttggtg
    24121 tagtcaccct tagagaaata gctgtttgtt cgatgtctat ttcttggaat tcaaataatt
    24181 gttcacacag atacactttt ctaaatatat ttcgctcgat gtaattttcc agcattcctg
    24241 atacacgcct ccagtttttc ttatgatttt atttaatcat atctgtaaat cgtatttttg
    24301 tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa
    24361 gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt tggactttta
    24421 ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct ttgactacat
    24481 ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc gggctgaaga
    24541 cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca ccgctttttg
    24601 caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca gcagttgtca
    24661 taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca ttccaaggag
    24721 caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg gtgacattga
    24781 gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg atcatttgat
    24841 tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat tcagaatgat
    24901 agatttcttc attcaatgtg atattaggat catcacagat ctctttcata aatgacaaga
    24961 ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc
    25021 ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata ttcatgatag
    25081 aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt tctattccgt
    25141 tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg gcaaagcgca
    25201 catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc gccataccta
    25261 attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt cctgtttgat
    25321 aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt tgaataacca
    25381 gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat
    25441 ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat cccatgctgt
    25501 ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa
    25561 agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg ataggctctt
    25621 tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat aaagtacaac
    25681 atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt attccccccg
    25741 ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc gatcatcaaa
    25801 tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct
    25861 gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca
    25921 cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt
    25981 cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt
    26041 actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat
    26101 acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc atgactggca
    26161 gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat
    26221 ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa
    26281 gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca
    26341 aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata
    26401 gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca
    26461 atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact
    26521 gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga
    26581 ctgacccaag tatagacacc gcctttttcc catcctttca ccgttgccat ctctgctgcg
    26641 cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa tactaagctg
    26701 aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga gatggtcata
    26761 gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct ttgttgcatg
    26821 aatcttcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa gaagaaaaag
    26881 tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat
    26941 tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac accaatcact
    27001 tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt agatgcaaag
    27061 atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca cacatcaagt
    27121 gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac gatacccgca
    27181 atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg cgtaatggct
    27241 gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt tccttttact
    27301 tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat
    27361 gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca
    27421 aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg acttctttct
    27481 cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc gaatccagct
    27541 gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc caaaaatccg
    27601 ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa aataacaagt
    27661 ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa aggaacggta
    27721 atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt tgcaccatga
    27781 aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg gtttccaata
    27841 acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca cataatcgtt
    27901 gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt cttccgtgct
    27961 aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc tgtacataaa
    28021 atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt cgcctccttt
    28081 ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt tttgaattaa
    28141 tacttgcatt tataaaagac agacaattaa taaaagcttg atgtgttgtg ctctttttcg
    28201 tttttcacac aaataaagac aaaaaataaa cggtcttctt tttatagaaa accgtttatt
    28261 ttcgtctttt tttattttta aagatatttt ttcataagga ataaa
//
LOCUS       DASJPH010000032        28109 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJPH010000032 DASJPH010000000
VERSION     DASJPH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.032"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..185)
                     /locus_tag="U3995_001215"
     CDS             complement(<1..185)
                     /locus_tag="U3995_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1796544.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            455..817
                     /locus_tag="U3995_001216"
     CDS             455..817
                     /locus_tag="U3995_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1796545.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            869..2551
                     /locus_tag="U3995_001217"
     CDS             869..2551
                     /locus_tag="U3995_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1796546.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2668..4704
                     /gene="recG"
                     /locus_tag="U3995_001218"
     CDS             2668..4704
                     /gene="recG"
                     /locus_tag="U3995_001218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1796547.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4753..5754
                     /gene="plsX"
                     /locus_tag="U3995_001219"
     CDS             4753..5754
                     /gene="plsX"
                     /locus_tag="U3995_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1796548.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5876..6118
                     /gene="acpP"
                     /locus_tag="U3995_001220"
     CDS             5876..6118
                     /gene="acpP"
                     /locus_tag="U3995_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1796549.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6386..7390
                     /locus_tag="U3995_001221"
     CDS             6386..7390
                     /locus_tag="U3995_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796550.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7391..8335
                     /locus_tag="U3995_001222"
     CDS             7391..8335
                     /locus_tag="U3995_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796551.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8335..9297
                     /locus_tag="U3995_001223"
     CDS             8335..9297
                     /locus_tag="U3995_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796552.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9324..10238
                     /locus_tag="U3995_001224"
     CDS             9324..10238
                     /locus_tag="U3995_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796553.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10264..12045
                     /locus_tag="U3995_001225"
     CDS             10264..12045
                     /locus_tag="U3995_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1796554.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12393..13079
                     /locus_tag="U3995_001226"
     CDS             12393..13079
                     /locus_tag="U3995_001226"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1796555.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13099..16680
                     /gene="smc"
                     /locus_tag="U3995_001227"
     CDS             13099..16680
                     /gene="smc"
                     /locus_tag="U3995_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1796556.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16689..17498
                     /locus_tag="U3995_001228"
     CDS             16689..17498
                     /locus_tag="U3995_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1796557.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17509..18507
                     /gene="ftsY"
                     /locus_tag="U3995_001229"
     CDS             17509..18507
                     /gene="ftsY"
                     /locus_tag="U3995_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1796558.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18694..20961
                     /gene="gshAB"
                     /locus_tag="U3995_001230"
     CDS             18694..20961
                     /gene="gshAB"
                     /locus_tag="U3995_001230"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1796559.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21050..21502)
                     /locus_tag="U3995_001231"
     CDS             complement(21050..21502)
                     /locus_tag="U3995_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1796560.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21652..22602
                     /locus_tag="U3995_001232"
     CDS             21652..22602
                     /locus_tag="U3995_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1796561.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22595..23554
                     /locus_tag="U3995_001233"
     CDS             22595..23554
                     /locus_tag="U3995_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1796562.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23551..24306
                     /locus_tag="U3995_001234"
     CDS             23551..24306
                     /locus_tag="U3995_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796563.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24329..25282
                     /locus_tag="U3995_001235"
     CDS             24329..25282
                     /locus_tag="U3995_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1796564.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25926..26222)
                     /locus_tag="U3995_001236"
     CDS             complement(25926..26222)
                     /locus_tag="U3995_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1796565.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26577..26933
                     /locus_tag="U3995_001237"
     CDS             26577..26933
                     /locus_tag="U3995_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1796566.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26943..27842
                     /locus_tag="U3995_001238"
     CDS             26943..27842
                     /locus_tag="U3995_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1796567.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         9299 a         4942 c         6086 g         7782 t
ORIGIN      
        1 acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt
       61 ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc
      121 atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt
      181 gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca
      241 gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata caattgacaa
      301 ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag
      361 tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa
      421 atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc
      481 tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga
      541 tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat
      601 tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc
      661 tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt
      721 ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa
      781 tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca
      841 gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt
      901 ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc
      961 attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac
     1021 tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat
     1081 gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt
     1141 gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt ctgatcttgc
     1201 tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg
     1261 aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac
     1321 tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa
     1381 aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg
     1441 actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga
     1501 aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag
     1561 tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt
     1621 aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta
     1681 tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca
     1741 cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt
     1801 caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga
     1861 agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg tagcgatcgc
     1921 tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg
     1981 tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc
     2041 agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc
     2101 agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac
     2161 agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga
     2221 aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga
     2281 cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc
     2341 taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac
     2401 agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc
     2461 tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc
     2521 tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca
     2581 gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt
     2641 ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg
     2701 gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa
     2761 gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa tctaaacgag
     2821 atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat
     2881 cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc
     2941 aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc
     3001 gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa gattttaggt
     3061 tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg
     3121 acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat
     3181 ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt
     3241 atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa
     3301 gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg
     3361 aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca aaagctacct
     3421 tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt
     3481 ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc
     3541 gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca
     3601 gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta
     3661 accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa
     3721 ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca agatggagtc
     3781 gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag
     3841 cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg
     3901 attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa
     3961 cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt
     4021 gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg
     4081 ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt
     4141 cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag
     4201 gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact
     4261 actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg
     4321 gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg cggaagtgat
     4381 gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa
     4441 atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt
     4501 cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg
     4561 gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcaaata
     4621 aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat
     4681 caggataatc gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa
     4741 ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca
     4801 attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac
     4861 ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat
     4921 acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact
     4981 gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca
     5041 gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat
     5101 atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc
     5161 gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt
     5221 attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc
     5281 ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc
     5341 cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat
     5401 ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt
     5461 gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc
     5521 aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg
     5581 gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact
     5641 catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt
     5701 gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg
     5761 gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta
     5821 aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac
     5881 tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac
     5941 cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat
     6001 tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc
     6061 tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt
     6121 tcaaataaaa gcaaagaagg ataaaaagct gcatgcagct ttttatcctt ctttttttaa
     6181 tctttttgac acgtaaaagt catttttctg ataattctgg cgataaacag aaattatgtt
     6241 gcaaagtaaa gcggttttct atataattat taataattca ttacacaaag ataagaataa
     6301 cctttgttat aaaacgattt attctgtgta atgtcctttt ttattaagaa aaacttccat
     6361 ttaggaagca gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct
     6421 acatacagga ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac
     6481 tttagacaaa aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt
     6541 ggctactacg attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt
     6601 atataacgat ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa
     6661 tgatatcgga atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga
     6721 agaccagatc aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc
     6781 tcgtgcacta gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca
     6841 atatccccat gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc
     6901 ctgcaaacct ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca
     6961 agcacagatt cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt
     7021 gatcactcat gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg
     7081 cggacagttc gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta
     7141 tacacgctct ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt
     7201 gatcgaaggt gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc
     7261 acgtattcca tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc
     7321 accgaaccac ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg
     7381 agaggtgtag atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg
     7441 cagtggattc ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat
     7501 tatcgaaaaa ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac
     7561 gggaaaagcc gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa
     7621 agatgtaacg aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat
     7681 cttccaagat tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga
     7741 acccatccga aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact
     7801 gctagatatc gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg
     7861 cggacaacgt caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt
     7921 agccgatgaa cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat
     7981 gaaacgaatc cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt
     8041 agtaaaacac atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg
     8101 tacaagagaa gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc
     8161 tattccgcaa atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga
     8221 acaagagtat atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt
     8281 gcgtacgatc acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg
     8341 aaaacgattc tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg
     8401 atcttcttat tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat
     8461 caggatccgg cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat
     8521 gtccagtact tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta
     8581 tacaaacgct ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg
     8641 atcttgacag ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg
     8701 tatcaaaatt caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt
     8761 cctttattta tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc
     8821 ccaacaagtg gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac
     8881 cgtttgaaat atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca
     8941 atccaatatc tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc
     9001 cgttccaaag gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg
     9061 ttgccgatgg cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt
     9121 atcgaaaaga tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa
     9181 cgggactata ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg
     9241 acattacttt cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac
     9301 caatcagaaa ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta
     9361 ttcctcctat ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt
     9421 tttcattagg tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg
     9481 atcaagacaa agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt
     9541 cgacacaagg acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac
     9601 aattgatcat aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt
     9661 cgatcatcgg ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca
     9721 tcttgatgcg gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct
     9781 tcgtatcgat tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt
     9841 attgggtagc taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt
     9901 atgtcagtgc ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca
     9961 tgccgaatct aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg
    10021 gtatcgagac aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag
    10081 gtacattgat cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt
    10141 tacccgcatc aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat
    10201 tcaagcgttc tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata
    10261 ggaatgaaga aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca
    10321 tgcgggggcg gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa
    10381 gaggaattaa cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa
    10441 ttagaagctg cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac
    10501 caagattcat atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat
    10561 gacgatttca gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa
    10621 aaagcgacaa tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg
    10681 gacgatgtga ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat
    10741 gacgacactt tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca
    10801 atctccggaa tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac
    10861 ccaagcatgc ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta
    10921 gaaggtgtag caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact
    10981 tttggccctt attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac
    11041 gagcactact ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg
    11101 gcaagtgcaa ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca
    11161 gatacttacg atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact
    11221 tcttatactt atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa
    11281 tacaatccag attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta
    11341 gataataatg cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg
    11401 attcctccag tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta
    11461 gacaaagcga aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta
    11521 agagaagaca aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa
    11581 acagcgcaac cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc
    11641 caatacacaa caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat
    11701 gatcctgaaa ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt
    11761 ggactatggg gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca
    11821 aaattgatca acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca
    11881 tatcaaaaat ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt
    11941 aatgaagtat tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca
    12001 aacccatggg caaatgtttc tgttacagca gattcacgtt cataatcact aaaaatgacc
    12061 gaaaaagggc gatgtccttt ttcggttttt tgatattttt ctgtttattt ctctaatatt
    12121 caggtcaagg cgctgatttc atgacttctc caataaacgg tgttcaaagt ccagtttctc
    12181 ggtattaagt caaatttatg caaaaatatg gaaagccatt ttcacaaaat tttttcttca
    12241 tactcgaaac taaacgaatt tttcacaact tctcgtcaat cactaggatt attaaagctt
    12301 tacttacctt ttgaccgaac catttcaaat ttgacaaaaa accagtataa taaggaagta
    12361 tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa ccagctgaca aaagaactaa
    12421 aagaaaacta taacatcgtt tttcacgatc taaatttgct tgaacaagct tttacccatt
    12481 catcctatgt gaatgagcat cgcaatctgc aattatcgga taatgagcga cttgaatttt
    12541 taggagatgc cgttttagaa ctgatggttt ctcagtattt attccgttta tatcctcatt
    12601 tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga
    12661 aatttgccaa agaatgtcac tttgatcaat atgttttgtt gggcaaggga gaagagaatt
    12721 caggaggacg tacgcgacca gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt
    12781 tatatcttga ccagggattc gacacgaccc atgcattcat cgaaaaagtg atttttccta
    12841 aagtcaaagc aggtgctttt tcacatgaga tggatcataa aacaaaacta caagaagtct
    12901 tgcaaaaatc aggtgatgta tcaatcgaat accgattgat caatgaagaa ggtccagctc
    12961 atgaacgagt attttggatc gaagtttatg tagatgatca attgattggc actggacaag
    13021 ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa tgcattggca gcactgtaaa
    13081 agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt taaatcattt
    13141 gcagacaaga cagtcattga ttttgaaaat agcgtcacag cagtggttgg accaaatggc
    13201 agcggaaaaa gcaatattac agaggctatc cgctgggtat taggagaaca atctgctaaa
    13261 agtctgcgag gcggaaaaat gccggatatc atctttgccg gttccgatac ccgtaagcag
    13321 ctgaatattg cagaagtcac agtgatttta gacaatagtg atcattatct gccattggag
    13381 tacaatgaga tcagtgtaac ccgccgttat cgacggactg gggaaagcga gtttttcatc
    13441 aataaacaaa gctgcagatt aaaagatatc caagaattgt tcttagattc tggattagga
    13501 aaagaatcgt tctcaatcat ttctcaagga aaagtagaag cgattttcag tagtaaaccg
    13561 gaagaccgtc gtgggatctt tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa
    13621 aagaaagcag aacagaagct ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc
    13681 atccacgaat tggaagaaca gttaactcct ttggccgccc agagtgaggc agcaaaggag
    13741 tttttacggc taaaagaaac actgactcaa accgatgtct ctttgatggt tgctgaaatc
    13801 aagacagcaa aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt caatctagaa
    13861 ttaggcaaac tatctgagtc gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa
    13921 gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc aagtattatt agatctttcg
    13981 gaaaaactga agcagacaga aggacaaaaa gatgttttgc aggagcggac aaagcataca
    14041 caaaaaagct cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat
    14101 tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac agagatccaa
    14161 aaagctgaag caaatctcat aaaaacacag caagaattag aaaaatacca aaaatcaacg
    14221 aaagaattat tggcagaatt aagagatcaa tatgttgatt tgatgcaaga acaagcagct
    14281 gtaggaaacg aactgaagta tctagaacgt caatatatac aagaaacagc aaaaagcaag
    14341 cagacgttag ccaagcaatc ggaagtagaa gcatcagtag accgattgat cttacaaaaa
    14401 gaagaattga cacaaaagca ggcgcagcta aaatcatctt taactgaaac aaaagagaag
    14461 ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa agaacagcct
    14521 aaaatgtatc aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa
    14581 gaaatccaag aaaactattt tggtttttac caaggggttc gattggtctt gcagcataaa
    14641 cagcagcttt cgggtattgt cggagcagtg gctgaactga tcgatgttcc tgcagatttc
    14701 acgttagcta tcgaagcagc tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa
    14761 aaagatgctc ggcaagccat cacgtatttg aaacagcaaa gaggaggaag agcaacattc
    14821 cttccactga cgacaatcaa accaagacaa ttaccggctc atatattgac acaagcagca
    14881 gctgtagaag ggtttatagg gattgccagt gaacaagtca gttatcccga tcaaatccaa
    14941 actgtggttc aaaacctgct aggaacgatc cttttagcca aagacttaac tagtgccaat
    15001 gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt ccttagaagg tgatgtgatg
    15061 aatgccggag gatccatgac aggtggagcg aataaacgag gaaaccaagg cagtttgttt
    15121 gtacaaaatc aagaattgaa acagttgact agtgaatttg aagaagcaga taaacaactc
    15181 caagcacaag aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt agccgaagaa
    15241 caagaagttt tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga agcgacaaat
    15301 cagctacaaa acatcataaa tgaacttgaa cgttttgaaa aagaaaagca aatctcaact
    15361 tttgagacac gtgaactgca gcagtttatc gaagattacg aaaagcaaac gaacgaattg
    15421 aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg
    15481 ttaagtcaag aaagcgacca gatggaagcc agaagagcac aagtacaaag tcaaaaagca
    15541 caagaacaag cagatcttgc tgtcttgaaa gagcagttta atcatctgca gatccagctc
    15601 cgcggtgccc gagtccaaaa agcagaggca accgaacgtc aagaagcgat agaaaagcag
    15661 ttggcgacat tgacagcaga cttttctgat catgaagtca cagaagaaag tttggaaaaa
    15721 caaattaacg aattatctgc tcagcgagaa acattaaaag ccgagttggc aaaagccaaa
    15781 gagcagcgag atagaaggca aaaggagatc gataagcttg aagcagtctt ggcagagcgt
    15841 aatcgtgagc aaaaagcacg actgtcggaa caatccaaat tagaagtcca aaaagaccga
    15901 gctgaaatga ttttagataa tcatttgacc tatctgcagt cagaatatca gctgacattt
    15961 gagaaagctt cccaagacta tcaagaaaca accgatatcg aagacagtag aacaaaggtt
    16021 tcatccttaa aagaacagat tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa
    16081 tacgaacaag tcagtgaaag acatacattc ttagcaacac agcgagatga tctgttagca
    16141 gcgaaaaacc aattgtttga aacgatggat gagatggatg atgaagtgcg gacaagattc
    16201 aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc
    16261 ggcggtaaag ctgaattagt actgacggaa ccaactgatt tgctgaaaac aggtattgag
    16321 attgaagtac aacctcctgg caaaaaactg caaagtctaa gtttgctttc tggaggagaa
    16381 cgagcactta cagccatcgc gttgctcttt tccattatcc gtgtctgtcc tgttccgttt
    16441 tgtatcttag atgaggtaga agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt
    16501 tacttgagcg atttccaaga tgacacacaa tttatcgtag tcacacaccg caaaggaacg
    16561 atggtcgcag cagatgtact gtacggagtg acgatgcagg aatccggtgt atcaaaaatc
    16621 gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag
    16681 gaacgaaaat gatcaaatta gtagcaatcg atttggacgg tacattatta aatgaacaaa
    16741 agctgatttc agatgagaat aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga
    16801 tcgttctatg tacaggacgc cccttagctg cgatggcgca ttatctgcaa gaattaggac
    16861 tggtagatga aggtgactac agcatcactt tcaacggcgg tttggtacaa aaaaatgata
    16921 ctggtgaaat catggaaaag aaagtgatgg aagtatcaga tatccaacgc ttatacaaac
    16981 tggctcagca attggatctg ccactagatg ttttgtctga caatgtggtt ttacagctgc
    17041 ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa taagctgctg caattccaac
    17101 cagcggatat aagcgatatt acagatgaat ttgtgcttaa taaggcagta atcgcttatg
    17161 accaagaaga attagatcct aaaatcaaag aaataccagc agaattccat gaaatgtatg
    17221 agatcatcaa aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt
    17281 atggtatttc tttattggca aaagatcttg gtttgaaaca agaagaaatc atggcgatcg
    17341 gtgatgagga aaatgattta ccgatgatcc aatatgctgg actaggcgta gcgatggaga
    17401 atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac agccagcaat gaagaaaatg
    17461 gcgtagccaa agtgatccaa aaatttgtat tagagtaggg ggagtcaaat gggatttttt
    17521 gataaaatca agcgtgcttt tactggcgaa gagaaaacag aagaagttga agtacaggag
    17581 aagtacgaca agggtttaga aaagacgcgt aaaacatttg gtgaacggat gaatgagttg
    17641 tttgccaatt ttcgaacagt cgatgaagac ttttttgaag aattagaaga aacacttatc
    17701 ggtgcagatg taggttttga aacagcgata cgaatcaccg aagcattacg tcaagaagtg
    17761 aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg
    17821 gatctctatg aagcggaagg aatcaatgaa gtaaatgaac taaatatcca agaagacgga
    17881 ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag tattggtaaa
    17941 cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc agataccttc
    18001 cgcgctggag caatcgacca gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt
    18061 cgtggcaatg caggaggcga tccagcagct gtcgtattcg atgctttgga aagagcaaag
    18121 acagagcagg cagatgtttt actagttgat actgcaggcc gactgcaaaa taaagtgaac
    18181 ttgatgaacg aattagaaaa aataaaacga gtaatccagc gggaattacc agaagcaccc
    18241 caagaaacac ttcttgtatt agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa
    18301 caattcaaag aaacaacaga tgtgaccggt cttgttttga caaaattaga tggtacagca
    18361 aagggcggta tcgtacttgc tatccgtaat gaattgcatt taccagtgaa attggtcggt
    18421 cttggtgaag gaatcgatga cttggagccg tttgatccaa atgattttgt cgttggattg
    18481 ttcaaaggat tactaaaaga agaataaccc atgtctaaaa agcaggagct gtgacaaaag
    18541 ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat gccttcattt
    18601 ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga ctttttgatt
    18661 ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca attattattg
    18721 catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg ggaaggacag
    18781 cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt tggcgatcga
    18841 tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat gatcacccct
    18901 gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga tgtgactgct
    18961 cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc cttaccggaa
    19021 aaagacgaag agatcattat tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt
    19081 tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa gcggtattca tttcaatttt
    19141 gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt tcaagatttt
    19201 tccgaattca aaacagaact ttatttgaaa acagcgagaa actttatgcg ctatcgctgg
    19261 atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt tttagatgaa
    19321 tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat
    19381 ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat cgagcgaatg
    19441 atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg tttccgaggc
    19501 ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt gcggaatatc
    19561 gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt cttacaatta
    19621 ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg ggtagcagaa
    19681 ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac agaatttcat
    19741 caagaaggta gagaaattct tgaaggaatg aagcaaatgc tggttgaatt ggattggttg
    19801 gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga acaaacgtta
    19861 gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc tgtggcattg
    19921 ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg ttttcgcgaa
    19981 atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa
    20041 gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt agaatatgtg
    20101 aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat ggaaaacaaa
    20161 acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg cgcagaattt
    20221 tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc atttgtgatc
    20281 aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg agctagtctg
    20341 gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt tttagtagaa
    20401 gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt ccaagccatc
    20461 atgttgcgag tccctgctaa tgttattggt gacagtatcc gaacagtgaa agaacttgtt
    20521 gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt agaattgatc
    20581 caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat cgaatcagtt
    20641 ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac aggtggtgat
    20701 tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt ttctgcggta
    20761 gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa agaaatcgat
    20821 cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc agccatgcat
    20881 atgcatgttt atccttttgc aggaaaaggc agaagattga cgatgaatgt attgaaattg
    20941 ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat ttgatgtcaa
    21001 tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt tttttcgttt taaaataaac
    21061 gatcccagaa gctttttttc ttttttggtt tttctttttc agctggcgaa gagttttctt
    21121 ctgggtattt tttttcgata tcaatcacag gttcatttac tgcttctttt gctgttaaga
    21181 gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt gactacagtg aaacggctat
    21241 tgtttttcgt tgctagctgg atatattggt tttggacaga aaggggtaga gccccattca
    21301 agagtatgga agcatctgga tgttttttga gttcttttaa aaagttttct ttgttttttg
    21361 gttggatcat ttcagcaaca gtcatactaa gatagcagcg ttccctgaat gtccctaaat
    21421 atttatgctg ttcttcaggg ttcactaaag gtgtaccata aagtccctta tccaaatggc
    21481 tttttacatc atcttgtgcc ataaaaaata ccctccttct tctctttgtc tttcttacga
    21541 ttatagcata ttcccaataa tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg
    21601 attcacttaa aataagaatg aaaatcattc tcaattagaa aggtggattg gatgcgctac
    21661 ggacgactag tagcattatt agttatttta agtagtattt cattgttcat cggagtatcg
    21721 aatgtacaat tgtcagaact gctccagtta gatgggcaaa gcttacaaat cttgctagtg
    21781 actcgtttgc cacgaacgat ttgtttgatc cttgtgggag cgaccagtag tatttgtggg
    21841 ttgatcatgc aacatttgac ccaaaataaa tttgtttcac caacgactgc gggcacgatg
    21901 gatagtgcgc gtttaggtat tttagtggct atgattttcc tacctggtgc atccttgatt
    21961 gtccgatcct tgacagcatt ttgctttgct tttgcaggaa cattgctttt tttatccctt
    22021 acgcgattgt tcctccagaa aaatcaattg atgcttccgt tgattggagt catgttaggg
    22081 aatatcattg gttcagttgc tacttttttt gcttatcaat tccagttggt acaaaatatg
    22141 acttcatggc ttcaagggaa ttttgcgact gtgatacgag gaaattatga gttattgtat
    22201 gctgttgttc cattgttgct cttggtttgg ctatttgctt accgatttac gatcattggt
    22261 ctgggcgaat cgtttgcagt aaatctggga atagattacc agaagatgca gtttttaggc
    22321 attggcttag tatcattggc tagtgcagtc atgttgatca tggtaggaag cattcctttt
    22381 atcggagtga tcattccgaa tcttgtctct agaatatatg gagatcaagt gcacaaaaca
    22441 attggcgtaa ctgctttagc aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc
    22501 gtgttgattt ttccttatga aataccagtc agtgtgatcg ttggaatcat cggtggggct
    22561 atcttccttt atcttctgat aaggggacga cgatatgctt aaaaaaaagc catctatttt
    22621 tattggtatg cttgttcttg ttttatttgt tacaggaatg tacttgacga tccatacata
    22681 tggaaactgg cattttgcat gtgcattgag gagtaagaaa atttttgcat ttatccttgt
    22741 ctcgctagct gtcagcagtg ccacaatcag ctttcagaca attacgcata accagttttt
    22801 gactccttca gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt
    22861 ctttatcgga ggagtgacga tgcttcagca gacaggtaca gtttttttct tattgaacgt
    22921 tgggctgatg gtcgggctga gtctattgtt atttttcttt cttttgaaaa aaatgagagc
    22981 gaatcttttt ttattactga tgtcagggat gattttaggt acgctttttg gtagtgtcag
    23041 taccttttta caagtcatca tggatccgaa tgaatatgat ttgcttcaag gcagattatt
    23101 tgcaagcttt ggcaatatcc agagtcagta tcttttgcct gcttctttga tcattatcgg
    23161 ctgctggtac ctgttatttc gatatcgaca tgtgctagac gttttccatc tcggaaatga
    23221 acaagcagta aacctcggga tagacacaga tcgttttgaa tttttgattt tatgttgtgt
    23281 cagtcttttg acagggacat caacggctct tgtgggtcca gttacttttt tagggtttat
    23341 tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt catggtcagc tatttatcgg
    23401 aagctttctg gtagctatgc tgtttctgac aggaggacag ctcgcagtag agcagatttt
    23461 cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac
    23521 aaaattgatt cgcgaaagga agagtatcct atgattgatt tacaaaaggt caataaactg
    23581 taccatggaa aatcagtggt taaagatgta cagttaacga ttcctgaaaa gaagttgact
    23641 gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg
    23701 ataccaaaag atacaggtga aatctattta gaccataacg aagtaaaggc ttggaaatcc
    23761 aatgaattag ccaaaaaatt atcgatccta cgacaatcga atgaaataaa tgtccaaatc
    23821 accgtgaaag aattagtaga atttggacga tttccttata cgaaaggaaa attgacgaca
    23881 aaagacgagg aaatcgttca ggaatcactg gaacacattg ggttagaaga aatagcggat
    23941 caatcgattg acactctttc aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta
    24001 gcgcaagaca cggattatat tttactggat gaacctttga acaatctaga tatgaatttt
    24061 gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt
    24121 attgttcttc atgatatcaa ctttgctgca agttatgcag acgaaattgt tgcaatgaaa
    24181 gatggacagt tatttacgca aggaacgacc caagaaatga taaaaaaaga tgtattggat
    24241 gaattatacg aaatggacat acggatttgt gagattgaag gccgcagatt ctgtctttat
    24301 ttctaaaact aacattagga gagaagagat gaaaaaatta gtagtaggta tcttgattct
    24361 agcatcattt ggtttagcag catgtggaaa tagcaacaac accagtcagg cagacacaaa
    24421 aaaaagcagc acacaaactg aaactacatt gacgataaca gatagtaatg gagaccaaat
    24481 cgaggtgcca aataatccag aaaaagtcgt cgtattcgac aatggttctt tagatacgat
    24541 ggatgctctt ggtgttggag ataaggtagt tggagcagct acaagcagcc ttcctgagta
    24601 tctttcatcc tataaaaaag tagaatcagc aggtggaata aaagaaccag acttagaaaa
    24661 aattaatcag cttcagccag atctaatcat catttctggc cgacaaagag attttcaaaa
    24721 agatctaagt caaattgctc cgaccatttt cctttcttta gatgcgaaaa atccatggga
    24781 atctttccag caaaatgtca ctgctttagg ggagatcttt ggtaagcaag aagaagctaa
    24841 aactcaatta gaggaattat ctagtgcaat cgatcagacg aaaaagaaag cagaagctac
    24901 tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa ttatcagcct acggatctgg
    24961 ttctagattc ggtttcatcc atgatttatt tggctttgaa caagcagatg atcaaatcga
    25021 agcatccact cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa atccggatat
    25081 cttatttgta gtagacagaa caaaagctat tggtggagac gacagcaaag atgatatttc
    25141 tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat cagcagatca tctcccttga
    25201 accagatgtc tggtatttaa gcgggggcgg tttagaatca atgaaactga tgatcgaaga
    25261 tgtgaatcaa gcatttaaat agcataaata acagaggatt aaactctcat actatgtaac
    25321 aataatgttt cttacattct taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac
    25381 catccgtaaa atcgtattga tgattttacg gatggttttt atttatttga gaaatctttt
    25441 ttcaacgtga attcattatc tacatcatac gggttgattc tagaatgggg agtggcaaaa
    25501 aatagtgtga tccatttttt cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac
    25561 aaagcgaaac ttagataaaa cttaaattta gatattttta ttttgtaccg atctcttctt
    25621 taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga aaatatgaga
    25681 agctattttc tgtccagcgt tcttattact caaaacaaat cgcagttgtc acaatctctt
    25741 ctaaacgatg ttccatctgc tgcttctcgg caataagccc actagactag aaaatatgag
    25801 aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct
    25861 tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga tttcacaacc
    25921 tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat ttttcgataa
    25981 aaatcggcgg gaagcttact ccaccgtttt agtaattgat agaaataact ctcttctgct
    26041 ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga agctaatttt
    26101 ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt tacttcaata
    26161 taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat atattccttc
    26221 atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga agagttgtta
    26281 ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt tttatttttt
    26341 atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg ttgctttttg
    26401 ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct tttgtgataa
    26461 gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt cagaaccaga
    26521 caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg atacagatga
    26581 aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa gcagacgatc
    26641 aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa gtcagttgcc
    26701 aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc
    26761 tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac agtgcaatac
    26821 tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt ttacaattaa
    26881 aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac tagagggata
    26941 aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa ttcggcgaat
    27001 cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg cttcatctag
    27061 ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata
    27121 aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg cccaatgaac
    27181 agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt gctttaaatg
    27241 agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc gaattgaaag
    27301 attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc atccagcgtg
    27361 ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc
    27421 agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct aaacgggcat
    27481 ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca tttttaggat
    27541 cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta gtttttggcc
    27601 atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc tgcagacctt
    27661 ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa cagttgctag
    27721 acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg
    27781 cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc gttccgtatt
    27841 gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga taagtaagag
    27901 cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc agcgaatgtt
    27961 tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta ttcttttttt
    28021 gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata attaatattg
    28081 attgaagtct aggcagtcaa gtcgagagt
//
LOCUS       DASJPH010000033        28050 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJPH010000033 DASJPH010000000
VERSION     DASJPH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.033"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..638)
                     /locus_tag="U3995_001239"
     CDS             complement(57..638)
                     /locus_tag="U3995_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1796568.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(821..1447)
                     /locus_tag="U3995_001240"
     CDS             complement(821..1447)
                     /locus_tag="U3995_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1796569.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1469..2800)
                     /locus_tag="U3995_001241"
     CDS             complement(1469..2800)
                     /locus_tag="U3995_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1796570.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2955..3356)
                     /locus_tag="U3995_001242"
     CDS             complement(2955..3356)
                     /locus_tag="U3995_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1796571.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3442..3648)
                     /locus_tag="U3995_001243"
     CDS             complement(3442..3648)
                     /locus_tag="U3995_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796572.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3743..5245)
                     /locus_tag="U3995_001244"
     CDS             complement(3743..5245)
                     /locus_tag="U3995_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1796573.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5263..5718)
                     /gene="argR"
                     /locus_tag="U3995_001245"
     CDS             complement(5263..5718)
                     /gene="argR"
                     /locus_tag="U3995_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1796574.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5816..6433)
                     /locus_tag="U3995_001246"
     CDS             complement(5816..6433)
                     /locus_tag="U3995_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1796575.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6654..8027
                     /locus_tag="U3995_001247"
     CDS             6654..8027
                     /locus_tag="U3995_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796576.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8102..9214
                     /locus_tag="U3995_001248"
     CDS             8102..9214
                     /locus_tag="U3995_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1796577.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9286..10923)
                     /locus_tag="U3995_001249"
     CDS             complement(9286..10923)
                     /locus_tag="U3995_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1796578.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11091..11699)
                     /locus_tag="U3995_001250"
     CDS             complement(11091..11699)
                     /locus_tag="U3995_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1796579.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12083..13234)
                     /gene="queG"
                     /locus_tag="U3995_001251"
     CDS             complement(12083..13234)
                     /gene="queG"
                     /locus_tag="U3995_001251"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1796580.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13253..14005)
                     /locus_tag="U3995_001252"
     CDS             complement(13253..14005)
                     /locus_tag="U3995_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796581.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14146..15096)
                     /locus_tag="U3995_001253"
     CDS             complement(14146..15096)
                     /locus_tag="U3995_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1796582.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15097..15936)
                     /locus_tag="U3995_001254"
     CDS             complement(15097..15936)
                     /locus_tag="U3995_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1796583.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15933..16679)
                     /locus_tag="U3995_001255"
     CDS             complement(15933..16679)
                     /locus_tag="U3995_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1796584.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16917..18287
                     /locus_tag="U3995_001256"
     CDS             16917..18287
                     /locus_tag="U3995_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796585.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18342..19238)
                     /locus_tag="U3995_001257"
     CDS             complement(18342..19238)
                     /locus_tag="U3995_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1796586.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19352..19522)
                     /locus_tag="U3995_001258"
     CDS             complement(19352..19522)
                     /locus_tag="U3995_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796587.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19519..20196)
                     /locus_tag="U3995_001259"
     CDS             complement(19519..20196)
                     /locus_tag="U3995_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1796588.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20287..21192)
                     /locus_tag="U3995_001260"
     CDS             complement(20287..21192)
                     /locus_tag="U3995_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1796589.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21367..22821
                     /locus_tag="U3995_001261"
     CDS             21367..22821
                     /locus_tag="U3995_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1796590.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22837..23868
                     /locus_tag="U3995_001262"
     CDS             22837..23868
                     /locus_tag="U3995_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1796591.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23875..25062
                     /locus_tag="U3995_001263"
     CDS             23875..25062
                     /locus_tag="U3995_001263"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1796592.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25218..26600
                     /locus_tag="U3995_001264"
     CDS             25218..26600
                     /locus_tag="U3995_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1796593.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26727..26957
                     /locus_tag="U3995_001265"
     CDS             26727..26957
                     /locus_tag="U3995_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796594.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27157..27825)
                     /locus_tag="U3995_001266"
     CDS             complement(27157..27825)
                     /locus_tag="U3995_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1796595.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7805 a         5742 c         5152 g         9351 t
ORIGIN      
        1 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
       61 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
      121 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
      181 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
      241 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
      301 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
      361 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
      421 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
      481 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
      541 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
      601 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
      661 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
      721 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
      781 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
      841 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
      901 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
      961 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     1021 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     1081 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     1141 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     1201 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     1261 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     1321 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     1381 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     1441 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     1501 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     1561 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     1621 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     1681 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     1741 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     1801 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     1861 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     1921 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     1981 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     2041 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     2101 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     2161 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     2221 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     2281 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     2341 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     2401 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     2461 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     2521 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     2581 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     2641 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     2701 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     2761 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     2821 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     2881 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     2941 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     3001 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     3061 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     3121 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     3181 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     3241 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     3301 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     3361 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     3421 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     3481 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     3541 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     3601 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     3661 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     3721 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     3781 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     3841 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     3901 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     3961 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     4021 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     4081 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     4141 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     4201 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     4261 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     4321 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     4381 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     4441 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     4501 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     4561 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     4621 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     4681 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     4741 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     4801 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     4861 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     4921 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     4981 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     5041 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     5101 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     5161 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     5221 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     5281 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     5341 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     5401 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     5461 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     5521 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     5581 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     5641 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     5701 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     5761 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     5821 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     5881 cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga
     5941 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     6001 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     6061 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     6121 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     6181 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     6241 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     6301 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     6361 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     6421 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     6481 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     6541 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
     6601 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
     6661 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
     6721 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
     6781 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
     6841 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
     6901 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
     6961 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
     7021 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
     7081 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
     7141 catggaatca tatcttcctg tttcttgtgc ccgttttgtt tctttctttg attatcggct
     7201 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
     7261 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
     7321 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
     7381 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
     7441 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
     7501 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
     7561 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
     7621 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
     7681 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
     7741 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
     7801 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
     7861 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
     7921 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
     7981 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
     8041 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
     8101 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
     8161 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
     8221 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
     8281 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
     8341 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
     8401 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
     8461 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
     8521 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
     8581 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
     8641 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
     8701 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
     8761 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
     8821 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
     8881 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
     8941 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
     9001 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
     9061 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
     9121 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
     9181 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
     9241 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
     9301 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
     9361 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
     9421 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
     9481 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
     9541 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
     9601 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
     9661 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
     9721 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
     9781 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
     9841 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
     9901 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
     9961 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    10021 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    10081 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    10141 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    10201 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    10261 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    10321 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    10381 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    10441 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    10501 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    10561 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    10621 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    10681 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    10741 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    10801 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    10861 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    10921 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    10981 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    11041 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    11101 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    11161 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    11221 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    11281 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    11341 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    11401 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    11461 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    11521 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    11581 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    11641 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    11701 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga
    11761 aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat
    11821 ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc
    11881 aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat
    11941 tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta
    12001 aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact
    12061 ttgaataatg ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt
    12121 ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa
    12181 gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga
    12241 tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta
    12301 gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag
    12361 ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt
    12421 ttggatatat ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt
    12481 gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt
    12541 tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg
    12601 cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac
    12661 agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct
    12721 caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa
    12781 atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg
    12841 gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga
    12901 tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag
    12961 caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa
    13021 gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat
    13081 cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt
    13141 cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt
    13201 tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc
    13261 tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg
    13321 caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg
    13381 aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac
    13441 ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat
    13501 acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc
    13561 caggcctagc aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa
    13621 taaattgact tttataatag gccgactgga tctttttgat tactccttct gctttgatga
    13681 ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc
    13741 caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta
    13801 aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg
    13861 tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt
    13921 gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat
    13981 atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg
    14041 aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct
    14101 tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt
    14161 ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc
    14221 tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc
    14281 cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac
    14341 cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg
    14401 actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata
    14461 cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact
    14521 ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg catttttttt
    14581 atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt cctccacata
    14641 ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat gttcttcacc
    14701 gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt ttccttcttc
    14761 ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa gattcaaacc
    14821 gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta gtacttcata
    14881 ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat ctgttccgac
    14941 ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact tttctttact
    15001 tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca agaaactaac
    15061 tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt tctgaaataa
    15121 aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac atgctaacac
    15181 gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga caactgcact
    15241 tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg ttagcaagta
    15301 agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta ctgtctgtaa
    15361 actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt
    15421 cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa
    15481 gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca aatcgctttc
    15541 cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac tctgggcata
    15601 tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc
    15661 atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta gcatcccaaa
    15721 agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta cacctggaag
    15781 aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa taaaagaacc
    15841 gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg
    15901 ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc ttttactaaa
    15961 tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac ttcttgtact
    16021 ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata ttcttctact
    16081 ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag
    16141 atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc atctaaaaag
    16201 atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag ctgtccaccc
    16261 gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt caaagcttgt
    16321 cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt
    16381 cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc acttcgctgt
    16441 tccacgtagg ctattttttt acggactgag tttaccgact gtccagaaaa ggtgacttta
    16501 ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt tcctgcaccg
    16561 tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat agttttcaaa
    16621 acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt acgttccaaa
    16681 tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta actaaaatgg
    16741 aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg cattttatga
    16801 aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat gaaaaacatg
    16861 tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg
    16921 aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc gcactaggtg
    16981 gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg acagggccct
    17041 cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg cgtgctttag
    17101 gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg agcgagtaca
    17161 tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat attgtcgtag
    17221 gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct gatttgccgg
    17281 caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca
    17341 aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg atcattttga
    17401 tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt caggcagtag
    17461 ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa ggatttttct
    17521 ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga atgactgctg
    17581 gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact atcggtcgaa
    17641 tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg tggaatcaat
    17701 tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt acatttgctg
    17761 caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat tcagggattt
    17821 tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct aaaaaatttc
    17881 tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca ggtggaatct
    17941 taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca gaaaatattt
    18001 tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta gtgattttga
    18061 tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat ccttttaaaa
    18121 tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc gtattgattt
    18181 tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt ttcttgcttt
    18241 acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag
    18301 cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa ttttagcgtc
    18361 tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa gatcgtttct
    18421 actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa aactacggtt
    18481 gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt tgtgtcttgt
    18541 gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga atcagaagat
    18601 gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg caaataaata
    18661 tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat caaagtatct
    18721 aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt tctgatccct
    18781 tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga taaaccagct
    18841 attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc aggcgtcttt
    18901 tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg caaaccattt
    18961 gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc acttgtttgt
    19021 ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa
    19081 ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa cacggcaact
    19141 gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc
    19201 atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac caatttttat
    19261 gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg tcgctgtgca
    19321 catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc caagattgtc
    19381 cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc agtttcttta
    19441 tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca tctaattgtt
    19501 ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa
    19561 gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt gtttttcaga
    19621 ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt caatccgctg
    19681 agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta gcttctccca
    19741 acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa gataggtcat
    19801 atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg tatattcttc
    19861 ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga ttcctagttc
    19921 tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa tttttaaatg
    19981 agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat gctgggccaa
    20041 gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt ccaaatgatg
    20101 ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta aagattcttc
    20161 gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt tttagtcttc
    20221 gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc attttaacca
    20281 gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt tcaaggtatc
    20341 caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg tagccgtttc
    20401 tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc ctacttgata
    20461 ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc gattgatccg
    20521 taccccgatc aaatattcct tgatactggt acccatatga tttttgaata ctcgcgacaa
    20581 atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt tttcagcata
    20641 atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct tttgtacacc
    20701 accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg ttaatgctgt
    20761 taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta aaataatgtc
    20821 gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga aaagcgactg
    20881 ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca tccacggatc
    20941 ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca gatcaccttt
    21001 tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca tcacgacatg
    21061 caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg ttttagagaa
    21121 accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt ttaaaaaaat
    21181 cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa aagttaaata
    21241 aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag tcattaaaag
    21301 atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag gagaggaata
    21361 cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc ttttggacac
    21421 gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag ccagatgttc
    21481 gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag cttagttgta
    21541 ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat cgataatact
    21601 cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt tagttccgta
    21661 ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac gactttattt
    21721 atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt taaggatatc
    21781 gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga aaaatttgct
    21841 acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt cgttgtccct
    21901 atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc ttcaggctgg
    21961 ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat cactgccttg
    22021 ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat
    22081 gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct
    22141 tatgcgattg ccaatgcagc aacaactgcc gtattattct acttcttcaa atatgtactt
    22201 ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg tttagtagct
    22261 gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta ccttggaggg
    22321 atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag tttgccaatc
    22381 gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt atccgctttg
    22441 atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg taacgaagct
    22501 gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc taacgggatc
    22561 gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc tgatatcact
    22621 gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc tttgatgatt
    22681 ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca tgcacaaatc
    22741 gttgagaaat tggaaaacaa attggctcca gctgatacaa tggaagatga acttcacgat
    22801 gcagtagatc cttcccatta agaaaggatc ttgaaaatga agattacaga aaaagatttt
    22861 ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact ttctatctct
    22921 gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt tttgggattt
    22981 gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc
    23041 gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca attagccaca
    23101 gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg
    23161 tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac tacaagtccc
    23221 gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac tttgacaaaa
    23281 gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt attcaatccg
    23341 acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga tcaacatgag
    23401 ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc tataggggta
    23461 aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt
    23521 tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc atttttctta
    23581 aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa aagaatccaa
    23641 gtagatattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt cggcacagaa
    23701 acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt tgagactcaa
    23761 acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa agccggatca
    23821 gtatttgaaa cagtaacaga attcaaaatc aaaacaagaa aggaataact catcatggaa
    23881 atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa aactacacaa
    23941 tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa tggaggccat
    24001 gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg agacgataat
    24061 aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt tactttagat
    24121 gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat ggtccttaaa
    24181 ttaaaagaag ctggacactc aatcgatcat gggtttgagt tattagtgga aggaacaatc
    24241 cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg tgttgtatta
    24301 gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg taaaaaagta
    24361 gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat cggcttcggt
    24421 gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat ggttccagtg
    24481 aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga attggcagat
    24541 tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact
    24601 ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac tattgaccaa
    24661 atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa tcagcggact
    24721 ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca actgttaaat
    24781 gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt agatacacta
    24841 gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg agctggattt
    24901 ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc
    24961 tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc acacattgga
    25021 aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa tcaacgatct
    25081 cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg agccttttgc
    25141 tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt tctaattgtg
    25201 ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat gaaaaagaac
    25261 cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta gagtgcgttg
    25321 aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg tccgattcga
    25381 cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg
    25441 gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt gatttatata
    25501 ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat tctatctata
    25561 aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc ttccttgatg
    25621 taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct aaactgatcc
    25681 ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc gataacccgc
    25741 tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg tttgctgaat
    25801 ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact gacttttggg
    25861 gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct gtttatgatt
    25921 tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt actcagcttt
    25981 atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca ccaaaatcaa
    26041 aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa gaaatcgaga
    26101 tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa
    26161 cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca aatggcaaga
    26221 ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat ctagcagatg
    26281 ctttattgac cattttccac cagcattacc actaccagga tattcggcgt gtaggcgtga
    26341 actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa
    26401 agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa aaatacggct
    26461 ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc gcacgtagtt
    26521 ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa gatgagacgg
    26581 acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg attaaaatgg
    26641 gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaatgaa gaagaagcac
    26701 gcaaacagcc tcttcctaaa caacaaatga cattgcaaga aatcgatagt atactgacag
    26761 aagccttttt atctcgtaaa gcagtaatca ttcagctcaa tttaaaagat gaatttggaa
    26821 gactcttgga tgaggtcgaa gggcacttca ttggtgaagc ctatgaagat tactttatga
    26881 ttgaccatca gccgatattg tgggaagacg tacggtatat tgagttaaag gaaacaaaaa
    26941 aatggtttgt gacatgagag gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga
    27001 tttttcgaaa attgcttctt aaatttttgt ggaattttgg cttatttttc gaagaagcaa
    27061 cttctggaac aacgtatatt agatatcaga ggttgtgaaa aagtcgctta gttttgagta
    27121 acaagccaag gcgctgattt cacaacctct gtctatctat ccatctattc ggtaagattc
    27181 atgcatgacg tcttctttat ttagtatgta gagccctttt tcttctgacc attcaacata
    27241 aatcagctgt tttacatctg catagccttc tttcttcaag ttctccataa gccatgcttc
    27301 agtcaattga ttggactgaa gcactttatg ctgaatttgc ccttcatcta caagcaggat
    27361 cgataaagct ttgtcttcat ctgatttttt caatacactc atttgaccgg cattctctag
    27421 gataacatgt ttggcatttt caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa
    27481 ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat
    27541 tagaacagat ggctcgcctt tgacccaacg attgaccttt tcattttttt ggacgatacg
    27601 ttcgatacca taaatcatta ctgcccaaag cgcaatggca aataggacat gccctacatg
    27661 gacattgtca tcataggttg attcttctaa gatcccacct aaaacaagcg tatagactaa
    27721 atcaaacggt gtgatttctg acatggattt cttcccaagc aaacgtacca ctaataataa
    27781 accgatcaac ccgaacacca gtttcattgt aattgaaaaa atcatttttt ctccccctaa
    27841 ctatctgtta attaaattat atatgctttc tcacgaacac gcatggaata tacaaggaag
    27901 aaatgtatcc ttttactaaa aaaaggactg tgacactgta gtgaccccca aaagttagac
    27961 ttttttggag tggagaaatc cactcctttt ttttatgctc taaaatgata tttttacaca
    28021 accgttacgg ttgttttacg aaattggacg
//
LOCUS       DASJPH010000034        28016 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJPH010000034 DASJPH010000000
VERSION     DASJPH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.034"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..119
                     /locus_tag="U3995_001267"
     CDS             <1..119
                     /locus_tag="U3995_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1796596.1"
                     /translation="AKNLAEKLGIHLTAESTEGKGTTMTLFFPILNFYNEKR"
     gene            208..1074
                     /locus_tag="U3995_001268"
     CDS             208..1074
                     /locus_tag="U3995_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1796597.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            1111..2487
                     /locus_tag="U3995_001269"
     CDS             1111..2487
                     /locus_tag="U3995_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796598.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(2588..3784)
                     /locus_tag="U3995_001270"
     CDS             complement(2588..3784)
                     /locus_tag="U3995_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1796599.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(3908..4570)
                     /locus_tag="U3995_001271"
     CDS             complement(3908..4570)
                     /locus_tag="U3995_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1796600.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            4798..5361
                     /locus_tag="U3995_001272"
     CDS             4798..5361
                     /locus_tag="U3995_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1796601.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            5374..5658
                     /locus_tag="U3995_001273"
     CDS             5374..5658
                     /locus_tag="U3995_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796602.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            5677..6369
                     /locus_tag="U3995_001274"
     CDS             5677..6369
                     /locus_tag="U3995_001274"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1796603.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            6381..6926
                     /locus_tag="U3995_001275"
     CDS             6381..6926
                     /locus_tag="U3995_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1796604.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            7197..10475
                     /locus_tag="U3995_001276"
     CDS             7197..10475
                     /locus_tag="U3995_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1796605.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(11016..12923)
                     /locus_tag="U3995_001277"
     CDS             complement(11016..12923)
                     /locus_tag="U3995_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1796606.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            13053..13928
                     /locus_tag="U3995_001278"
     CDS             13053..13928
                     /locus_tag="U3995_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1796607.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            14008..14982
                     /locus_tag="U3995_001279"
     CDS             14008..14982
                     /locus_tag="U3995_001279"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1796608.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            15162..16307
                     /locus_tag="U3995_001280"
     CDS             15162..16307
                     /locus_tag="U3995_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1796609.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            16444..16788
                     /locus_tag="U3995_001281"
     CDS             16444..16788
                     /locus_tag="U3995_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1796610.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            17059..18345
                     /gene="efmA"
                     /locus_tag="U3995_001282"
     CDS             17059..18345
                     /gene="efmA"
                     /locus_tag="U3995_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1796611.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(18483..19082)
                     /locus_tag="U3995_001283"
     CDS             complement(18483..19082)
                     /locus_tag="U3995_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1796612.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            19486..20607
                     /gene="mnmA"
                     /locus_tag="U3995_001284"
     CDS             19486..20607
                     /gene="mnmA"
                     /locus_tag="U3995_001284"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1796613.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            20853..22274
                     /gene="arcD"
                     /locus_tag="U3995_001285"
     CDS             20853..22274
                     /gene="arcD"
                     /locus_tag="U3995_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1796614.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            22288..23406
                     /locus_tag="U3995_001286"
     CDS             22288..23406
                     /locus_tag="U3995_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1796615.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(23466..24758)
                     /locus_tag="U3995_001287"
     CDS             complement(23466..24758)
                     /locus_tag="U3995_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1796616.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            24890..25468
                     /locus_tag="U3995_001288"
     CDS             24890..25468
                     /locus_tag="U3995_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1796617.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            25597..25974
                     /locus_tag="U3995_001289"
     CDS             25597..25974
                     /locus_tag="U3995_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1796618.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            25907..27979
                     /locus_tag="U3995_001290"
     CDS             25907..27979
                     /locus_tag="U3995_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1796619.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         8830 a         5046 c         6011 g         8129 t
ORIGIN      
        1 tagcaaaaaa tctggccgaa aaattaggta tccatttgac ggctgaatca actgaaggaa
       61 aagggacaac aatgacttta tttttcccaa tcttaaattt ttataatgaa aaacgttaga
      121 aaatcgttag aaacgaaaaa aagtgcttgt gttgttaacg ttttcgctga taagtttctt
      181 atcatgtgaa ataaaaggag attggttatg gaaattttaa tagcattagt ccctatgttg
      241 gcatggggta gtattggttt agtaagtggt aaaattggtg gcgatgccaa tcaacaaaca
      301 ctaggaatga cgatcggtgc atttctgttt tctttagttg tctttttcgt tgttagtcct
      361 gtaatcaccc cttggatttt cttgatcgga tttttatccg gcttagcttg gagtgttgga
      421 caaaatggac agttccatgg aatgaagtac atgggtgttt ctgtagggct accattgtct
      481 actggttttc aattaatttt gaatacgatt gcgggagccg ttttcttcca tgaatggaca
      541 caaacgaaag attatgtata cggtattatt gctttagctc ttcttgtaag cggtgcgtat
      601 ttgactgccc gacaagatga cgaaggaaaa atagatacag ataataaaat gcttgatttt
      661 ggtaagggtt ttagagcatt gatcttctct acgattggtt atggtgtata taccatcatc
      721 gtcaactggg cgaatttgga tgcgatgtca attatcttac ctcaaagtat cggaatgatc
      781 ttaggcgcaa gcttctttgc tttccgtaaa gttaaagtcg atcagtttgt gtggaaaaac
      841 atgatctgtg gattattatg gggcttagga aacatctgca tgcttctaac agtcaaatca
      901 cttggtttag ctgttggctt ctcactttca caaatgggga tcattatctc tactttaggt
      961 gggattttca tccttggcga aagaaaaacg aaaaaagagc tcatttatgt tattgttggt
     1021 tgccttttag tcattttagg tggtatcctt ttagggtata tgaaaacagt ttaatcgctg
     1081 ttttccaacc taaaatgggg aggaacttca atggatttgt ttcgtaagaa agagatcaat
     1141 ctcaatcata cgagtgagat gaaaaaagaa ctaaaaacgt ctgatttgat catgcttgga
     1201 attggtgcga tcatcggtac gggaatattt gtggtgacag gggttgcggc caatcaaaat
     1261 gctggtccag cactgtcact ttcatttgtc ttagctgcaa tcgttgtcat tttgtctgga
     1321 ctaagtttcg cagaatttgc ttcgcgagtg ccagtgattg gtgggccata tgcctatctg
     1381 tatgttgtat ttggtgaatt tgctgcctgg ctgacagggt ggttgttgat cggggaattt
     1441 ttactagcgg tttcctctgt tgcttccgga tggtctggct atatgcaagg ttttttgaaa
     1501 agtttagggg ccgaacttcc acaagcatta actggcgggt acaatccgga aaatggtaca
     1561 tacattgact tgattgcggt gctcgtagtg atattcgtca cttacatcgt gtcgcttgaa
     1621 gccaagaaag cattacgttt gaataatgcc atggtatatg tcaaatttgg tatcatcgcg
     1681 ttgtttatca ttgtaggaat atttttcgtt aaacctgata actggcagcc attcatgcct
     1741 tttggttttt ccggtgtgct agatggtgca gctcttgtgt tttttgcttt cttgggattt
     1801 gatgccgttg caatggcagc cgaggaagtc aaaaacccgc aaaaagatgt gccaagagga
     1861 atcattggtt cgatactgat tgctactgtc ctttacatca tcgtgacgct gatcttgaca
     1921 ggaatcgttc cgtatactga actaggagtc aatgacccag tggcttttgc aatgaggtat
     1981 gtaggacatg gaacagtggg cgcggtgatc gctgtagggg caattttaac gctactgacg
     2041 gtaacgatct cgatgatgta ttcacttgct cgtttactgt ttgctgtcag caaggatggc
     2101 ttacttccta agtttatgac agaaatcgat caaaagcatc gtacaccgaa aaaagcgact
     2161 tatgccgctg gtgtggcagc tgtattcttt gccggatttt tcccattgaa tgtattagcg
     2221 gaactaacga atatcatggc gctcgcctat ttgatgcttg ttagtctagg cttactgaag
     2281 ttgagaaaaa tgtttggcaa accaaaagct ggagaattca aagtgccatt cgttccgcta
     2341 ttgccaatcg tctccatctt gacttgtatt gtattgatgc ttcgtctgca aactgtgaca
     2401 tggatcgtgt tcggtatcgt aatggttatt ggactgatca tttattttgg ctatggctat
     2461 ggacacagca agatgaatga gaaatagact ccaatggaga agttagaaga aagataagaa
     2521 aaacgcccat caacagttgt aataaactgt tgttgggcgt ttgttttagt aacaagcgaa
     2581 aggtgatcta ttgggttaaa tccaacagtt tttgtttcag atcttcttcc atgtgtccaa
     2641 gctctacttc tgcatgacga cgtttttctt ttccttcttt ttggatacgc aatgtctctt
     2701 ggattgtttc gatcaaatca ttttgagtgg tttgaagggt ctcgatatcc acgattccac
     2761 gttcattttc tttcgctgtt tcgatggctg agattttcag catttctgaa tttttcttca
     2821 ataagtcgtt tgttgtttca gagacttggc gttgagcagt tactgcatct ttttgacgaa
     2881 gcaaggtcaa tgcgatcacg acttggtttt tccacagcgg aatggctgta gcgatagatg
     2941 cttggatctt ttcagccaag gcctgatttg tgttttggat caaacggatt tgtggtgctt
     3001 gctggatcgt gatctgtcta gctaagcgaa gatcatgcgt acgtttatcc agacgatcta
     3061 ggaattgcgt gtaatcattg gcaatctgaa catccatctg atcgccagtt tcttctgctc
     3121 ttttcatcgc ttctggaatg atcgttgttt gaagttcttc cattttcaat tctccagcag
     3181 cgatataaat attcagtgca tcaaagtagt ctttgttttt ctggtaaagc tgttctaaca
     3241 tcagattatc ttttagcaga ccgtcttttt ctttatctaa tttcactgaa atcttgtcaa
     3301 tttgtgcacc aatcttttga tattttgctg tcacttcata gatcgattgt ttgaccttcc
     3361 cgaagacacg ctggaagatg ttcccttctc cggcacgcag ttcgtcagga ttggcttctt
     3421 ggagacgata catcagttca gttaaggaat ctcctacagg accaatatct tgtgcttgca
     3481 catgattcaa catcgattgt gagaattcac ttaattttgc ttgggcagcc gatccgtagc
     3541 tgatgacaga ctgtgcatca ttagcatcga tttttgctgc tagctgttta gcttgttctt
     3601 ggcgttccgc cggtagtttg tcaattaaac gagcagctgt ctgttgctct tggagctgat
     3661 caatctccgc ttgttgtgaa gtggtcagcg tatctgttgg agttgaaaac gggttgttca
     3721 ataaatcttc aagtgtatcg ttaactggtg taacatcttt tgtttcagtc ggtttatgat
     3781 ccataaaaaa ttcctccttg ttgaatctgt tctcgattct tacatgaatg tctctacttt
     3841 agaaaaagta ctcgaaacag ccagacatca tctgaatgtt ccgagtcttc ttttatgaac
     3901 aaaagagtta atccttttgt tctggttcat atttattatc acgctttaag ctctgtttcg
     3961 caacagatat ctctacgtcg atatcatcta gatcctcagc aacgaatttt tgataatctt
     4021 gtgcgattaa agctgacagc tggtcgatga tctgcgcgct ttcttctagt ttttcatagg
     4081 tctgtttgtt tttgatctca tggtcattga tctcattata cttatttgtc aaatcaacta
     4141 gattcggcag atgcgtataa agaaactgac ttgcttcatg cagtcggttt ggttctttca
     4201 ccaattcttt gaataaagct ttcgctgctt tgactggatc atggcggaga tcgattgctt
     4261 taagtttggc tgtctgctgc atattcattt gcaattgatt gatctcattt tttgtttgat
     4321 tcattgtttg tcgaaaaaat gaaatttcat tcgctgtcat accgcttttt tcatagtgag
     4381 tttcaagttc gtcagtcagt gcaggaattt cttcttgttt tccctttttc ctgcgcgaac
     4441 caaagagtcc ccacaaaact aaaactacac ctatcaataa aagtcctaga acaaaagaaa
     4501 aactattgtt tcggacaaag agaaatagca agaccaatgc tagaagccac ggccaatttc
     4561 gttttatcat aataaaccct ccatgtcatt tatcgttgag tattgaattc ttttcgttat
     4621 tcgattacat acttatttta gcatacggac tactagaaag agtatcggac taaagaagga
     4681 ttttctatat ttagagaaaa tctaaggttt atctatggta aactaataag gagtttttga
     4741 gaaaaattga aaaatgaaaa aaaagcagtt atgattacaa agaaagcagg agaagatatg
     4801 ctgaacaatg agcaatttga agaaaaaaca atcagtcgca aagaaatcta tagcggaaaa
     4861 attattgatg ttgctgtaga cgaagttcgt ttgccagatg gaggaacttc gaagagagaa
     4921 ctggtatttc atcctggcgg tgtcgggatc attgcttttg acgaacaaga tcgtctgctt
     4981 cttgtcaaac aatttcgaaa acctttagaa aaagtgattt tggagattcc agctggaaaa
     5041 atcgatccag gagaaggaca aaatcccgaa atgacagcag ctagagaatt agaagaagaa
     5101 actggctatc gtgcgaagtc gctgagccat cttacttcga tgtatctttc accaggtttt
     5161 gcaaatgaag tccttcatat ttatcatgca caaggggtag agaaggtcga gaatccttta
     5221 gcacaagatg aagacgaagt actggagttg tatcatctaa ctcttgaaga ggcgcaacaa
     5281 gcgatgaaag atcagttgat ttgtgatgca aagacgattt atgcgattca atactgggaa
     5341 ctattgacca aaggaaagta gagggatcgg cttatggcca aaggaccgct tattacgcga
     5401 agcgaacttc gcaagcgaca acaagcacaa gcaagcgagt cattaaaaaa acagcgaaaa
     5461 gcagaaacag cttatcagca agaagaaaaa aagattgcca gcttttatcg taaagaaagc
     5521 aaaaagaata aaccaatcac gaaaacaagg atcagcgaac gggaaaagac gaccaaatgg
     5581 aactcttttt tgatgaaatc tcttattatt gttatcctaa tgttatgtgt ggttttttta
     5641 gcaatcgcat ttatatagaa agaaggaatt aaaaagatga aaatcggtat tatcggcgca
     5701 atggaagaag aagtaaaaat tttgagagag aacctaagcg aaccattatc atgggaacgc
     5761 gcaggcgctt tatttatttc aggttcatta ggtaaccacg aagtgatcgt cgtacgttcc
     5821 ggtataggaa aagtcttagc atctattacc acaagtctgt tgatccaaca atacggcgtg
     5881 aacatggtga tcaatacagg ttcagctggc ggaatcggtg aaggcttaca agtaggtgac
     5941 atcgttatat cagacaaagt agcttatttt gatgcagatg caacaggatt tggctacaac
     6001 ccaggacaat taccaggaat gcctctatat tatgaagcaa gcacatattt gcgcagtgaa
     6061 atggttaaag cagcaaaagc aacgaatttg aacgcaaaag aaggattgat cgttacaggt
     6121 gatacatttg tcgactcgcc agataaaatc aaagaaatct taacgaattt ccctgaagca
     6181 ttagcttgcg aaatggaagg agccgctgtt ggacaaacag ctcgtcagtt caatatccca
     6241 tttttgatcg tacgtgcaat gagcgacaca gcagatcact cggcgacaca aagctttgac
     6301 gaatttatcg aagacgcagg caaacgatca gcagaaatgg tgatcgaatt tgtcaaacat
     6361 ctcgtataag gaggactaag atgaacgcat tgatctcgat tgactacaca tatgattttg
     6421 ttgcagagga tggaaagctg actactggga cagcaggaca agtaattgaa aaaaagctga
     6481 ctgctcatac aaagaagttt attgatcaaa aggattttgt cgtttttgcc attgatgcgc
     6541 atgatccaaa agattccttt catccggaga accgcttatt tcctccgcat aatgtgatag
     6601 gaacgggtgg acgggaattg tacggctctt taaaaagttt ataccaagac tatgagcaag
     6661 aggaaaatgt ttattggata gacaaacggc attattccgc ttttagtgga acagatctag
     6721 acatccgttt gcgagaacgc cagatcacag atatttattt gaccggcgta tgtacagata
     6781 tctgtgtgtt gcatacagca gtcgatgcgt ataatctagg atacaaactc catattttca
     6841 aagattccgt ggcaagtttc gatcccgttg gacatgaatg ggcattgaga cattttgagt
     6901 ccacactagg cgcagaaatt ttataaaaaa acaaagtgat ttgataaaat aaaaagcaca
     6961 aaataataaa agaagagcat aaaacaaacg actttgtttt atgctctttt ttatacgtta
     7021 ttttaaaaaa acttattctt tttccgttaa taaatctctt tattatcaac aacttaagtt
     7081 atattttttc tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa
     7141 taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt
     7201 taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg
     7261 ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg
     7321 cacacgtgaa cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata
     7381 tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa
     7441 agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg
     7501 ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg
     7561 gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca
     7621 aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg
     7681 cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc
     7741 aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact
     7801 cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg
     7861 ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga
     7921 gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag
     7981 gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta
     8041 ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc
     8101 ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt
     8161 cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag
     8221 aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta
     8281 ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc
     8341 atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag
     8401 gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc
     8461 agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact
     8521 atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag
     8581 tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag
     8641 gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg
     8701 ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa
     8761 aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa
     8821 acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact
     8881 actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga
     8941 tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa
     9001 tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca
     9061 agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg
     9121 ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag
     9181 atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca
     9241 aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta
     9301 atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt
     9361 ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata
     9421 gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa
     9481 acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag
     9541 agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt
     9601 ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga
     9661 tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg
     9721 gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg
     9781 aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt
     9841 cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac
     9901 aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata
     9961 tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc
    10021 ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa
    10081 gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc
    10141 tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat
    10201 ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg
    10261 acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac
    10321 cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa
    10381 aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg
    10441 tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta
    10501 aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc
    10561 cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact
    10621 tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc
    10681 ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata
    10741 ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct
    10801 cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac
    10861 tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc
    10921 ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct
    10981 tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct
    11041 ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg
    11101 ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa
    11161 agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat
    11221 tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct
    11281 gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc
    11341 agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct
    11401 tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat
    11461 gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc agagcttgat
    11521 tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga
    11581 aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact
    11641 ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt
    11701 gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg
    11761 atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac ggctttttct
    11821 tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt
    11881 tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag
    11941 taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga
    12001 ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat
    12061 tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat
    12121 aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt
    12181 acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt
    12241 ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct
    12301 gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt
    12361 tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc
    12421 atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc
    12481 ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc
    12541 atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga
    12601 aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga
    12661 agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca
    12721 aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt
    12781 tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt
    12841 ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct
    12901 tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta
    12961 tcttacaaaa tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa
    13021 actgatttgt ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga
    13081 atcaagtagt ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca
    13141 gcactcagca gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag
    13201 atcaaagtta tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat
    13261 atacttatca agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg
    13321 agttatctag atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt
    13381 caggaaagga actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata
    13441 agaaatgggc taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg
    13501 cctattatca agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata
    13561 cggctgattc gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc
    13621 ctacgattcc ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc
    13681 atgagcgtgc acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg
    13741 aaggcaaacc tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt
    13801 gggtgacagc gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg
    13861 acaactgggg cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac
    13921 ttccttaaga atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa
    13981 gaatagagaa gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag
    14041 atttttagtt tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca
    14101 gaattaggta aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa
    14161 gaaagtattc gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat
    14221 tactatatgg aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc
    14281 aatgtcgtat tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa
    14341 ccgatcactg cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg
    14401 actttagatc tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta
    14461 tttacgatgc cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc
    14521 gtagttgttt cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg
    14581 aacagcacat tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg
    14641 atcggtgatg ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca
    14701 acgcttgcac gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt
    14761 gcgtcacatg gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa
    14821 gatatttgta tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca
    14881 atcgttacat gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg
    14941 atgagtccgt tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa
    15001 gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc
    15061 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
    15121 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
    15181 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
    15241 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
    15301 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
    15361 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
    15421 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
    15481 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
    15541 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
    15601 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
    15661 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
    15721 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
    15781 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
    15841 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
    15901 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
    15961 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
    16021 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
    16081 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
    16141 cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag
    16201 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
    16261 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
    16321 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
    16381 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
    16441 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
    16501 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
    16561 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
    16621 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
    16681 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
    16741 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
    16801 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
    16861 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
    16921 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
    16981 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
    17041 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
    17101 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
    17161 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
    17221 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
    17281 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
    17341 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
    17401 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
    17461 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
    17521 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
    17581 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
    17641 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
    17701 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
    17761 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
    17821 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
    17881 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
    17941 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
    18001 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
    18061 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
    18121 gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca
    18181 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
    18241 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
    18301 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
    18361 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
    18421 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
    18481 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
    18541 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
    18601 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
    18661 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
    18721 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
    18781 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
    18841 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
    18901 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
    18961 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
    19021 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
    19081 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
    19141 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
    19201 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
    19261 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
    19321 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
    19381 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
    19441 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
    19501 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
    19561 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
    19621 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
    19681 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
    19741 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
    19801 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
    19861 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
    19921 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
    19981 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
    20041 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
    20101 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
    20161 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
    20221 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
    20281 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
    20341 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
    20401 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
    20461 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
    20521 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
    20581 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
    20641 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
    20701 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
    20761 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
    20821 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
    20881 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
    20941 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
    21001 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
    21061 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
    21121 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
    21181 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
    21241 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
    21301 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
    21361 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
    21421 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
    21481 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
    21541 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
    21601 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
    21661 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
    21721 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
    21781 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
    21841 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
    21901 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
    21961 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
    22021 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
    22081 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
    22141 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
    22201 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
    22261 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
    22321 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
    22381 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
    22441 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
    22501 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
    22561 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
    22621 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
    22681 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
    22741 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
    22801 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
    22861 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
    22921 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
    22981 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
    23041 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
    23101 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
    23161 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
    23221 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
    23281 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
    23341 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
    23401 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
    23461 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
    23521 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
    23581 accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca
    23641 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
    23701 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
    23761 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
    23821 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
    23881 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
    23941 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
    24001 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
    24061 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
    24121 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
    24181 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
    24241 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
    24301 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
    24361 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
    24421 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
    24481 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
    24541 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
    24601 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
    24661 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
    24721 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
    24781 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
    24841 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
    24901 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
    24961 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
    25021 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    25081 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    25141 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    25201 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    25261 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    25321 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    25381 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    25441 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    25501 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    25561 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    25621 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    25681 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    25741 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    25801 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    25861 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    25921 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    25981 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    26041 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    26101 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    26161 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    26221 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    26281 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    26341 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    26401 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    26461 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    26521 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    26581 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    26641 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    26701 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    26761 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    26821 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    26881 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    26941 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    27001 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    27061 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    27121 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    27181 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    27241 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    27301 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    27361 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt
    27421 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    27481 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    27541 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    27601 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    27661 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    27721 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    27781 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    27841 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    27901 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    27961 agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagct
//
LOCUS       DASJPH010000035        27873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJPH010000035 DASJPH010000000
VERSION     DASJPH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.035"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..602
                     /gene="nrdI"
                     /locus_tag="U3995_001291"
     CDS             156..602
                     /gene="nrdI"
                     /locus_tag="U3995_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1796620.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(667..2649)
                     /locus_tag="U3995_001292"
     CDS             complement(667..2649)
                     /locus_tag="U3995_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1796621.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2663..3559)
                     /gene="murQ"
                     /locus_tag="U3995_001293"
     CDS             complement(2663..3559)
                     /gene="murQ"
                     /locus_tag="U3995_001293"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1796622.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3562..4623)
                     /locus_tag="U3995_001294"
     CDS             complement(3562..4623)
                     /locus_tag="U3995_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1796623.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4834..5400)
                     /locus_tag="U3995_001295"
     CDS             complement(4834..5400)
                     /locus_tag="U3995_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1796624.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(5428..6930)
                     /gene="eat(A)"
                     /locus_tag="U3995_001296"
     CDS             complement(5428..6930)
                     /gene="eat(A)"
                     /locus_tag="U3995_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1796625.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7188..8309)
                     /locus_tag="U3995_001297"
     CDS             complement(7188..8309)
                     /locus_tag="U3995_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1796626.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8435..9376
                     /locus_tag="U3995_001298"
     CDS             8435..9376
                     /locus_tag="U3995_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1796627.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9526..10422)
                     /locus_tag="U3995_001299"
     CDS             complement(9526..10422)
                     /locus_tag="U3995_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1796628.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10691..12031
                     /locus_tag="U3995_001300"
     CDS             10691..12031
                     /locus_tag="U3995_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796629.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12249..12692
                     /locus_tag="U3995_001301"
     CDS             12249..12692
                     /locus_tag="U3995_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1796630.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13196..14614)
                     /gene="nhaC"
                     /locus_tag="U3995_001302"
     CDS             complement(13196..14614)
                     /gene="nhaC"
                     /locus_tag="U3995_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1796631.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15017..16240)
                     /locus_tag="U3995_001303"
     CDS             complement(15017..16240)
                     /locus_tag="U3995_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1796632.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16271..17110)
                     /locus_tag="U3995_001304"
     CDS             complement(16271..17110)
                     /locus_tag="U3995_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1796633.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17127..17807)
                     /locus_tag="U3995_001305"
     CDS             complement(17127..17807)
                     /locus_tag="U3995_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796634.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17809..18882)
                     /locus_tag="U3995_001306"
     CDS             complement(17809..18882)
                     /locus_tag="U3995_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796635.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18938..19176)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19219..19452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19529..20200)
                     /locus_tag="U3995_001307"
     CDS             complement(19529..20200)
                     /locus_tag="U3995_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1796636.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20287..22965)
                     /gene="mgtA"
                     /locus_tag="U3995_001308"
     CDS             complement(20287..22965)
                     /gene="mgtA"
                     /locus_tag="U3995_001308"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1796637.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            23624..24061
                     /gene="ndk"
                     /locus_tag="U3995_001309"
     CDS             23624..24061
                     /gene="ndk"
                     /locus_tag="U3995_001309"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1796638.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24083..24949)
                     /locus_tag="U3995_001310"
     CDS             complement(24083..24949)
                     /locus_tag="U3995_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1796639.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25109..27202)
                     /locus_tag="U3995_001311"
     CDS             complement(25109..27202)
                     /locus_tag="U3995_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1796640.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         7978 a         5864 c         4913 g         9118 t
ORIGIN      
        1 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
       61 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
      121 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
      181 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
      241 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
      301 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
      361 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
      421 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
      481 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
      541 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
      601 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
      661 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
      721 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
      781 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
      841 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
      901 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
      961 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     1021 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     1081 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     1141 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     1201 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     1261 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     1321 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     1381 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     1441 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     1501 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     1561 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     1621 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     1681 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     1741 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     1801 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     1861 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     1921 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     1981 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     2041 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     2101 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     2161 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     2221 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     2281 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     2341 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     2401 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     2461 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     2521 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     2581 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     2641 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     2701 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     2761 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     2821 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     2881 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     2941 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     3001 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     3061 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     3121 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     3181 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     3241 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     3301 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     3361 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     3421 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     3481 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     3541 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     3601 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     3661 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     3721 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     3781 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     3841 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     3901 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     3961 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     4021 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     4081 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     4141 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     4201 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     4261 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     4321 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     4381 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     4441 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     4501 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     4561 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     4621 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     4681 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     4741 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     4801 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     4861 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     4921 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     4981 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     5041 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     5101 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     5161 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     5221 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     5281 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     5341 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     5401 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     5461 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     5521 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     5581 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     5641 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     5701 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     5761 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     5821 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     5881 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
     5941 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
     6001 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
     6061 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
     6121 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
     6181 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
     6241 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
     6301 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
     6361 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
     6421 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
     6481 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
     6541 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
     6601 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
     6661 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
     6721 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
     6781 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
     6841 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
     6901 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
     6961 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
     7021 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
     7081 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
     7141 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
     7201 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
     7261 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
     7321 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
     7381 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
     7441 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
     7501 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
     7561 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
     7621 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
     7681 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
     7741 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
     7801 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
     7861 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
     7921 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
     7981 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
     8041 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
     8101 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
     8161 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
     8221 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
     8281 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
     8341 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
     8401 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
     8461 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
     8521 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
     8581 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
     8641 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
     8701 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
     8761 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
     8821 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
     8881 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
     8941 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
     9001 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
     9061 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
     9121 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
     9181 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
     9241 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
     9301 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
     9361 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
     9421 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
     9481 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
     9541 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
     9601 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
     9661 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
     9721 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
     9781 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
     9841 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
     9901 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
     9961 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    10021 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    10081 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    10141 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    10201 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    10261 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    10321 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    10381 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    10441 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    10501 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    10561 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    10621 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    10681 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    10741 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    10801 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    10861 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    10921 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    10981 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    11041 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    11101 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    11161 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    11221 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    11281 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    11341 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    11401 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    11461 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    11521 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    11581 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    11641 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    11701 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    11761 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    11821 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    11881 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    11941 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    12001 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    12061 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    12121 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    12181 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    12241 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    12301 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    12361 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    12421 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    12481 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    12541 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    12601 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    12661 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    12721 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    12781 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    12841 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    12901 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    12961 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    13021 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    13081 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    13141 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    13201 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    13261 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    13321 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    13381 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    13441 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    13501 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    13561 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    13621 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    13681 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    13741 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    13801 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    13861 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    13921 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    13981 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    14041 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    14101 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    14161 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    14221 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    14281 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    14341 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    14401 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    14461 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    14521 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    14581 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    14641 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    14701 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    14761 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    14821 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    14881 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    14941 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    15001 acaagattat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    15061 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    15121 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    15181 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    15241 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    15301 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    15361 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    15421 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    15481 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    15541 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    15601 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    15661 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    15721 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    15781 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    15841 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    15901 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    15961 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    16021 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    16081 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    16141 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    16201 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    16261 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    16321 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    16381 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    16441 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    16501 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    16561 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    16621 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    16681 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    16741 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    16801 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    16861 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    16921 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    16981 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    17041 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    17101 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    17161 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    17221 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    17281 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    17341 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    17401 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    17461 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    17521 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    17581 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    17641 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    17701 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    17761 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    17821 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    17881 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    17941 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    18001 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    18061 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    18121 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    18181 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    18241 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    18301 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    18361 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    18421 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    18481 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    18541 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    18601 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    18661 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    18721 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    18781 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    18841 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    18901 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    18961 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    19021 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    19081 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    19141 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    19201 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    19261 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    19321 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    19381 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    19441 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    19501 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    19561 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    19621 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    19681 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    19741 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    19801 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    19861 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    19921 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    19981 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    20041 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    20101 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    20161 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    20221 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    20281 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    20341 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    20401 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    20461 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    20521 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    20581 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    20641 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    20701 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    20761 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    20821 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    20881 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    20941 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    21001 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    21061 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    21121 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    21181 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    21241 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    21301 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    21361 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    21421 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    21481 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    21541 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    21601 cagcatttct tcaatcgcac cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    21661 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    21721 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    21781 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    21841 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    21901 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    21961 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    22021 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    22081 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    22141 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    22201 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    22261 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    22321 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    22381 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    22441 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    22501 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    22561 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    22621 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    22681 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    22741 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    22801 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    22861 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    22921 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    22981 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    23041 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    23101 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    23161 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    23221 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    23281 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    23341 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    23401 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    23461 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    23521 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    23581 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    23641 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    23701 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    23761 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    23821 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    23881 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    23941 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    24001 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    24061 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    24121 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    24181 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    24241 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    24301 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    24361 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    24421 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    24481 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    24541 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    24601 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    24661 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    24721 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    24781 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    24841 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    24901 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    24961 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    25021 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    25081 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    25141 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    25201 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    25261 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    25321 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    25381 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    25441 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    25501 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    25561 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    25621 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    25681 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    25741 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    25801 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    25861 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    25921 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    25981 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    26041 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    26101 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    26161 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    26221 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    26281 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    26341 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    26401 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    26461 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    26521 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    26581 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    26641 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    26701 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    26761 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    26821 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    26881 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    26941 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    27001 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    27061 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    27121 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    27181 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    27241 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    27301 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    27361 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    27421 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    27481 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    27541 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    27601 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    27661 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    27721 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    27781 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    27841 atcagctgac cttcggtatt agctcaaaaa acg
//
LOCUS       DASJPH010000036        27762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJPH010000036 DASJPH010000000
VERSION     DASJPH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.036"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1921
                     /locus_tag="U3995_001312"
     CDS             155..1921
                     /locus_tag="U3995_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1796641.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2023..3282
                     /locus_tag="U3995_001313"
     CDS             2023..3282
                     /locus_tag="U3995_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1796642.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3429..4730
                     /locus_tag="U3995_001314"
     CDS             3429..4730
                     /locus_tag="U3995_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796643.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4733..5584
                     /locus_tag="U3995_001315"
     CDS             4733..5584
                     /locus_tag="U3995_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796644.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5597..6412
                     /locus_tag="U3995_001316"
     CDS             5597..6412
                     /locus_tag="U3995_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1796645.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6563..7729
                     /locus_tag="U3995_001317"
     CDS             6563..7729
                     /locus_tag="U3995_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1796646.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7959..8606
                     /locus_tag="U3995_001318"
     CDS             7959..8606
                     /locus_tag="U3995_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1796647.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8690..9634
                     /locus_tag="U3995_001319"
     CDS             8690..9634
                     /locus_tag="U3995_001319"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1796648.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGV
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9769..11220
                     /locus_tag="U3995_001320"
     CDS             9769..11220
                     /locus_tag="U3995_001320"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1796649.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11241..12077
                     /locus_tag="U3995_001321"
     CDS             11241..12077
                     /locus_tag="U3995_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1796650.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12077..12736
                     /locus_tag="U3995_001322"
     CDS             12077..12736
                     /locus_tag="U3995_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1796651.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12862..13551
                     /locus_tag="U3995_001323"
     CDS             12862..13551
                     /locus_tag="U3995_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796652.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13557..14513
                     /locus_tag="U3995_001324"
     CDS             13557..14513
                     /locus_tag="U3995_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1796653.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14557..15069
                     /locus_tag="U3995_001325"
     CDS             14557..15069
                     /locus_tag="U3995_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796654.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15325..15873)
                     /locus_tag="U3995_001326"
     CDS             complement(15325..15873)
                     /locus_tag="U3995_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1796655.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15962..17143)
                     /locus_tag="U3995_001327"
     CDS             complement(15962..17143)
                     /locus_tag="U3995_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1796656.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17379..18887)
                     /locus_tag="U3995_001328"
     CDS             complement(17379..18887)
                     /locus_tag="U3995_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1796657.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19046..19237
                     /locus_tag="U3995_001329"
     CDS             19046..19237
                     /locus_tag="U3995_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796658.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19440..20357
                     /locus_tag="U3995_001330"
     CDS             19440..20357
                     /locus_tag="U3995_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1796659.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20350..21423
                     /locus_tag="U3995_001331"
     CDS             20350..21423
                     /locus_tag="U3995_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796660.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21571..22089
                     /locus_tag="U3995_001332"
     CDS             21571..22089
                     /locus_tag="U3995_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796661.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22070..22462
                     /locus_tag="U3995_001333"
     CDS             22070..22462
                     /locus_tag="U3995_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1796662.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22538..23473
                     /locus_tag="U3995_001334"
     CDS             22538..23473
                     /locus_tag="U3995_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1796663.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23606..24385
                     /locus_tag="U3995_001335"
     CDS             23606..24385
                     /locus_tag="U3995_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1796664.1"
                     /translation="MNKKKSAPWLQALHAVMPLYISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24378..24695
                     /locus_tag="U3995_001336"
     CDS             24378..24695
                     /locus_tag="U3995_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1796665.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24939..26549
                     /locus_tag="U3995_001337"
     CDS             24939..26549
                     /locus_tag="U3995_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1796666.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26706..27554
                     /locus_tag="U3995_001338"
     CDS             26706..27554
                     /locus_tag="U3995_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1796667.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8978 a         4913 c         5563 g         8308 t
ORIGIN      
        1 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
       61 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gtttctctat cacttatagt
      121 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
      181 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
      241 taggaaaaat gatatcaaaa aagtatatgt gctttgtgga gatccatata ccataacaac
      301 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
      361 ttattgggaa attgaagtga cgagcgaaac aaggcgctta cagtatgcgt tccatgttgt
      421 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
      481 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
      541 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
      601 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
      661 ccgagaagac ttcttcgggg gagatttgca aggtgtgagg gatcatttgg attacttgac
      721 ggaccttggc gtcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
      781 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
      841 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
      901 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
      961 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     1021 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     1081 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     1141 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     1201 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     1261 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     1321 tgcattcaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     1381 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     1441 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     1501 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     1561 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     1621 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     1681 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag tatatgatcg
     1741 tgaagataag ctctatttca cacgggaact agagggacag atcatctatg tttactttaa
     1801 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     1861 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     1921 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     1981 taagggtgta acagcacgtt gaatacacag gaggaaatga agatgaaaaa aagaagtgtg
     2041 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     2101 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     2161 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     2221 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     2281 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     2341 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     2401 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     2461 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     2521 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     2581 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     2641 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     2701 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     2761 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     2821 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     2881 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     2941 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     3001 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     3061 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     3121 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     3181 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     3241 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     3301 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     3361 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     3421 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     3481 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     3541 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     3601 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     3661 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     3721 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     3781 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     3841 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     3901 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     3961 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     4021 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     4081 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     4141 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     4201 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     4261 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     4321 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     4381 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     4441 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     4501 atttcaataa tttctccatg atctatctgt ttaacaatgg tggtcccgga agcgtaggcg
     4561 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     4621 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     4681 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     4741 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     4801 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     4861 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     4921 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     4981 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     5041 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     5101 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     5161 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     5221 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     5281 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     5341 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     5401 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     5461 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     5521 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     5581 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
     5641 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
     5701 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
     5761 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
     5821 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
     5881 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
     5941 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
     6001 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
     6061 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
     6121 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
     6181 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
     6241 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
     6301 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
     6361 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
     6421 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
     6481 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
     6541 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
     6601 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
     6661 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
     6721 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
     6781 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
     6841 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
     6901 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
     6961 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
     7021 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
     7081 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
     7141 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
     7201 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
     7261 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
     7321 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
     7381 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
     7441 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
     7501 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
     7561 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
     7621 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
     7681 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
     7741 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
     7801 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
     7861 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
     7921 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
     7981 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
     8041 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
     8101 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
     8161 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
     8221 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
     8281 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
     8341 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
     8401 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
     8461 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
     8521 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
     8581 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
     8641 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
     8701 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
     8761 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
     8821 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
     8881 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
     8941 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
     9001 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
     9061 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
     9121 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
     9181 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
     9241 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
     9301 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg gtcggcggca ttggcggaga
     9361 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
     9421 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
     9481 atgtggcgca agcatgttac agattggtac agagcttcat aaagaagggc cggaaatttt
     9541 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
     9601 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
     9661 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
     9721 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
     9781 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
     9841 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
     9901 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
     9961 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    10021 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    10081 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    10141 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    10201 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    10261 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    10321 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    10381 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    10441 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    10501 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    10561 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    10621 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    10681 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    10741 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    10801 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    10861 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    10921 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    10981 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    11041 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    11101 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    11161 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    11221 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    11281 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    11341 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    11401 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    11461 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    11521 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    11581 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    11641 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    11701 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    11761 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    11821 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    11881 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    11941 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    12001 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    12061 agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact
    12121 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    12181 aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    12241 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    12301 tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    12361 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    12421 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    12481 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    12541 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    12601 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    12661 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    12721 gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    12781 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    12841 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    12901 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    12961 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    13021 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    13081 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    13141 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    13201 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    13261 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    13321 gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca
    13381 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    13441 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    13501 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    13561 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    13621 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    13681 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc
    13741 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    13801 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    13861 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    13921 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    13981 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    14041 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    14101 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    14161 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    14221 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    14281 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    14341 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    14401 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    14461 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    14521 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    14581 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    14641 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    14701 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    14761 gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    14821 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    14881 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    14941 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    15001 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    15061 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    15121 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    15181 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    15241 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta
    15301 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    15361 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    15421 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    15481 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    15541 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    15601 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    15661 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    15721 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    15781 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    15841 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    15901 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    15961 attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    16021 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    16081 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    16141 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    16201 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    16261 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    16321 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    16381 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    16441 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    16501 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    16561 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    16621 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    16681 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    16741 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    16801 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    16861 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    16921 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    16981 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    17041 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    17101 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    17161 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    17221 ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa
    17281 aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc
    17341 taatagtttc gttttcaaca tttataagta aactttttct attggtaata tagattttcg
    17401 gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca
    17461 tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca
    17521 taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca
    17581 ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt
    17641 tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact
    17701 gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg
    17761 acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct
    17821 tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa
    17881 ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt
    17941 ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg
    18001 acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat
    18061 tgataaacag attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc
    18121 tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg
    18181 atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt
    18241 ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct
    18301 ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca
    18361 ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca
    18421 tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag
    18481 accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta
    18541 tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc atcgattaac
    18601 cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata
    18661 tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg
    18721 attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc
    18781 ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct
    18841 gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct
    18901 taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc
    18961 tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga
    19021 tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa
    19081 acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga
    19141 acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg
    19201 cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga
    19261 ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt
    19321 ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa
    19381 aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta
    19441 tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg
    19501 atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga
    19561 atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata
    19621 tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa
    19681 atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg
    19741 aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg
    19801 gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg
    19861 ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg
    19921 acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
    19981 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
    20041 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
    20101 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
    20161 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
    20221 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
    20281 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
    20341 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
    20401 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
    20461 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
    20521 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
    20581 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
    20641 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
    20701 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
    20761 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
    20821 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
    20881 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
    20941 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
    21001 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
    21061 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
    21121 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
    21181 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
    21241 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
    21301 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
    21361 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
    21421 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
    21481 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
    21541 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
    21601 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
    21661 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
    21721 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
    21781 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
    21841 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
    21901 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
    21961 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
    22021 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
    22081 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
    22141 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
    22201 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
    22261 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
    22321 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
    22381 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
    22441 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
    22501 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
    22561 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
    22621 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
    22681 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
    22741 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
    22801 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
    22861 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
    22921 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
    22981 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
    23041 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
    23101 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
    23161 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
    23221 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
    23281 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
    23341 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
    23401 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
    23461 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
    23521 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
    23581 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
    23641 tttgcatgca gtgatgccac tttatataag ctatatacca gtaggattag cttgcggagt
    23701 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
    23761 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
    23821 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
    23881 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
    23941 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
    24001 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
    24061 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
    24121 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
    24181 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
    24241 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
    24301 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
    24361 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
    24421 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
    24481 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
    24541 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
    24601 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
    24661 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
    24721 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
    24781 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
    24841 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
    24901 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
    24961 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
    25021 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
    25081 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
    25141 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
    25201 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
    25261 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
    25321 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
    25381 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
    25441 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
    25501 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
    25561 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
    25621 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
    25681 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
    25741 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
    25801 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
    25861 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
    25921 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
    25981 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
    26041 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
    26101 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
    26161 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
    26221 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
    26281 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
    26341 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
    26401 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
    26461 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
    26521 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
    26581 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
    26641 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
    26701 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
    26761 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
    26821 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
    26881 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
    26941 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
    27001 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
    27061 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
    27121 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    27181 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    27241 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    27301 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    27361 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    27421 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    27481 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    27541 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    27601 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    27661 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    27721 tagtaaatga gtaaactact aatttactta tttactaaaa at
//
LOCUS       DASJPH010000037        27594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJPH010000037 DASJPH010000000
VERSION     DASJPH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.037"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(178..2070)
                     /locus_tag="U3995_001339"
     CDS             complement(178..2070)
                     /locus_tag="U3995_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796668.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="U3995_001340"
     CDS             complement(2067..3794)
                     /locus_tag="U3995_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796669.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="U3995_001341"
     CDS             complement(3810..4460)
                     /locus_tag="U3995_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1796670.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="U3995_001342"
     CDS             4664..4960
                     /locus_tag="U3995_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1796671.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="U3995_001343"
     CDS             5132..5752
                     /locus_tag="U3995_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1796672.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="U3995_001344"
     CDS             complement(5844..7010)
                     /locus_tag="U3995_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1796673.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="U3995_001345"
     CDS             complement(7022..7375)
                     /locus_tag="U3995_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1796674.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="U3995_001346"
     CDS             7503..8525
                     /locus_tag="U3995_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1796675.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8544..8705
                     /locus_tag="U3995_001347"
     CDS             8544..8705
                     /locus_tag="U3995_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796676.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8964..9398)
                     /locus_tag="U3995_001348"
     CDS             complement(8964..9398)
                     /locus_tag="U3995_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796677.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="U3995_001349"
     CDS             complement(9508..11367)
                     /locus_tag="U3995_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1796678.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="U3995_001350"
     CDS             complement(11485..12645)
                     /locus_tag="U3995_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN1796679.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="U3995_001351"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="U3995_001351"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1796680.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="U3995_001352"
     CDS             complement(14392..15090)
                     /locus_tag="U3995_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1796681.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="U3995_001353"
     CDS             complement(15047..16708)
                     /locus_tag="U3995_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1796682.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="U3995_001354"
     CDS             16926..18326
                     /locus_tag="U3995_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1796683.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="U3995_001355"
     CDS             complement(18578..19657)
                     /locus_tag="U3995_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1796684.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="U3995_001356"
     CDS             complement(19743..20702)
                     /locus_tag="U3995_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1796685.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22225)
                     /locus_tag="U3995_001357"
     CDS             complement(20714..22225)
                     /locus_tag="U3995_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1796686.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="U3995_001358"
     CDS             complement(22247..22810)
                     /locus_tag="U3995_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1796687.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="U3995_001359"
     CDS             complement(22816..23679)
                     /locus_tag="U3995_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1796688.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="U3995_001360"
     CDS             complement(23693..24589)
                     /locus_tag="U3995_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1796689.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26053)
                     /locus_tag="U3995_001361"
     CDS             complement(24764..26053)
                     /locus_tag="U3995_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1796690.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="U3995_001362"
     CDS             complement(26025..27542)
                     /locus_tag="U3995_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1796691.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8054 a         5829 c         4771 g         8940 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttg
//
LOCUS       DASJPH010000038        26522 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJPH010000038 DASJPH010000000
VERSION     DASJPH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.038"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="U3995_001363"
     CDS             <1..1152
                     /locus_tag="U3995_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN1796692.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="U3995_001364"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="U3995_001364"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1796693.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="U3995_001365"
     CDS             2728..3003
                     /locus_tag="U3995_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1796694.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="U3995_001366"
     CDS             3009..3776
                     /locus_tag="U3995_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1796695.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="U3995_001367"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="U3995_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1796696.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="U3995_001368"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="U3995_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1796697.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="U3995_001369"
     CDS             7920..8222
                     /locus_tag="U3995_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1796698.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="U3995_001370"
     CDS             8225..9388
                     /locus_tag="U3995_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1796699.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="U3995_001371"
     CDS             9414..12842
                     /locus_tag="U3995_001371"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1796700.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="U3995_001372"
     CDS             12863..13987
                     /locus_tag="U3995_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796701.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="U3995_001373"
     CDS             13977..14414
                     /locus_tag="U3995_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1796702.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="U3995_001374"
     CDS             14398..14736
                     /locus_tag="U3995_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1796703.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="U3995_001375"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="U3995_001375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1796704.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="U3995_001376"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="U3995_001376"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1796705.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="U3995_001377"
     CDS             15893..16936
                     /locus_tag="U3995_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1796706.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="U3995_001378"
     CDS             17095..17823
                     /locus_tag="U3995_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1796707.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="U3995_001379"
     CDS             17939..18184
                     /locus_tag="U3995_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1796708.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="U3995_001380"
     CDS             18181..18399
                     /locus_tag="U3995_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1796709.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="U3995_001381"
     CDS             18535..19074
                     /locus_tag="U3995_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1796710.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="U3995_001382"
     CDS             19067..21340
                     /locus_tag="U3995_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1796711.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="U3995_001383"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="U3995_001383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1796712.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="U3995_001384"
     CDS             complement(22513..22767)
                     /locus_tag="U3995_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1796713.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="U3995_001385"
     CDS             complement(23167..23436)
                     /locus_tag="U3995_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796714.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="U3995_001386"
     CDS             complement(23906..24361)
                     /locus_tag="U3995_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1796715.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="U3995_001387"
     CDS             complement(24499..26427)
                     /locus_tag="U3995_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1796716.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8825 a         4605 c         5604 g         7488 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tg
//
LOCUS       DASJPH010000039        26000 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJPH010000039 DASJPH010000000
VERSION     DASJPH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.039"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="U3995_001388"
     CDS             complement(164..388)
                     /locus_tag="U3995_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN1796717.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="U3995_001389"
     CDS             complement(586..2166)
                     /locus_tag="U3995_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1796718.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="U3995_001390"
     CDS             complement(2567..3943)
                     /locus_tag="U3995_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1796719.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="U3995_001391"
     CDS             complement(4071..5189)
                     /locus_tag="U3995_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1796720.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="U3995_001392"
     CDS             5331..5765
                     /locus_tag="U3995_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796721.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="U3995_001393"
     CDS             complement(5843..6376)
                     /locus_tag="U3995_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1796722.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U3995_001394"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U3995_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1796723.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U3995_001395"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U3995_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1796724.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="U3995_001396"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="U3995_001396"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1796725.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="U3995_001397"
     CDS             complement(10101..10679)
                     /locus_tag="U3995_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1796726.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="U3995_001398"
     CDS             11001..11471
                     /locus_tag="U3995_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1796727.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="U3995_001399"
     CDS             complement(11579..12805)
                     /locus_tag="U3995_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1796728.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="U3995_001400"
     CDS             complement(13559..13759)
                     /locus_tag="U3995_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1796729.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="U3995_001401"
     CDS             complement(13893..14501)
                     /locus_tag="U3995_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1796730.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="U3995_001402"
     CDS             complement(14586..15056)
                     /locus_tag="U3995_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1796731.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="U3995_001403"
     CDS             complement(15104..16018)
                     /locus_tag="U3995_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1796732.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="U3995_001404"
     CDS             16164..16967
                     /locus_tag="U3995_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1796733.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="U3995_001405"
     CDS             17145..18092
                     /locus_tag="U3995_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1796734.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="U3995_001406"
     CDS             complement(18160..18453)
                     /locus_tag="U3995_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN1796735.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="U3995_001407"
     CDS             complement(18481..19146)
                     /locus_tag="U3995_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1796736.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="U3995_001408"
     CDS             complement(19286..19918)
                     /locus_tag="U3995_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796737.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="U3995_001409"
     CDS             complement(19925..20992)
                     /locus_tag="U3995_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1796738.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="U3995_001410"
     CDS             complement(20989..21720)
                     /locus_tag="U3995_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1796739.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRT"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U3995_001411"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U3995_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1796740.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U3995_001412"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U3995_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1796741.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="U3995_001413"
     CDS             23883..25052
                     /locus_tag="U3995_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1796742.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..>26000)
                     /locus_tag="U3995_001414"
     CDS             complement(25319..>26000)
                     /locus_tag="U3995_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796743.1"
                     /translation="CTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQL
                     EKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYV
                     PIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTV
                     TVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIK
                     LKIRTSHE"
BASE COUNT         7860 a         5190 c         4163 g         8787 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atgttcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtacaa
//
LOCUS       DASJPH010000040        25452 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJPH010000040 DASJPH010000000
VERSION     DASJPH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.040"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..1523
                     /locus_tag="U3995_001415"
     CDS             87..1523
                     /locus_tag="U3995_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1796744.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1712..4159
                     /locus_tag="U3995_001416"
     CDS             1712..4159
                     /locus_tag="U3995_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1796745.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4389..5456
                     /gene="rlmN"
                     /locus_tag="U3995_001417"
     CDS             4389..5456
                     /gene="rlmN"
                     /locus_tag="U3995_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1796746.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5649..6752)
                     /locus_tag="U3995_001418"
     CDS             complement(5649..6752)
                     /locus_tag="U3995_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1796747.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7305..7853
                     /gene="aac(6')"
                     /locus_tag="U3995_001419"
     CDS             7305..7853
                     /gene="aac(6')"
                     /locus_tag="U3995_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1796748.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8399..10060
                     /locus_tag="U3995_001420"
     CDS             8399..10060
                     /locus_tag="U3995_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1796749.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(10130..10477)
                     /locus_tag="U3995_001421"
     CDS             complement(10130..10477)
                     /locus_tag="U3995_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1796750.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            10561..11502
                     /locus_tag="U3995_001422"
     CDS             10561..11502
                     /locus_tag="U3995_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796751.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            11503..12546
                     /locus_tag="U3995_001423"
     CDS             11503..12546
                     /locus_tag="U3995_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796752.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            12552..13622
                     /locus_tag="U3995_001424"
     CDS             12552..13622
                     /locus_tag="U3995_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796753.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            13612..14565
                     /locus_tag="U3995_001425"
     CDS             13612..14565
                     /locus_tag="U3995_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796754.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            14577..15587
                     /locus_tag="U3995_001426"
     CDS             14577..15587
                     /locus_tag="U3995_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1796755.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(15872..16693)
                     /locus_tag="U3995_001427"
     CDS             complement(15872..16693)
                     /locus_tag="U3995_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1796756.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(16790..17068)
                     /locus_tag="U3995_001428"
     CDS             complement(16790..17068)
                     /locus_tag="U3995_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796757.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            17336..19345
                     /gene="metG"
                     /locus_tag="U3995_001429"
     CDS             17336..19345
                     /gene="metG"
                     /locus_tag="U3995_001429"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1796758.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(19824..20411)
                     /locus_tag="U3995_001430"
     CDS             complement(19824..20411)
                     /locus_tag="U3995_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796759.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            20586..21908
                     /locus_tag="U3995_001431"
     CDS             20586..21908
                     /locus_tag="U3995_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796760.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            22063..22839
                     /locus_tag="U3995_001432"
     CDS             22063..22839
                     /locus_tag="U3995_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1796761.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            22832..23401
                     /gene="rnmV"
                     /locus_tag="U3995_001433"
     CDS             22832..23401
                     /gene="rnmV"
                     /locus_tag="U3995_001433"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1796762.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            23394..24278
                     /gene="rsmA"
                     /locus_tag="U3995_001434"
     CDS             23394..24278
                     /gene="rsmA"
                     /locus_tag="U3995_001434"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1796763.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            24729..25358
                     /locus_tag="U3995_001435"
     CDS             24729..25358
                     /locus_tag="U3995_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796764.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
BASE COUNT         8446 a         4463 c         5315 g         7228 t
ORIGIN      
        1 tttacataat tttattttgt tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt
       61 gaaggttata ggaaaggaag agatgaatgg aacacaaaga acttacctca tttccaaaag
      121 attttctgtg gggctctgct tctgcagcgt atcaagtaga aggagcttgg caagaagatg
      181 ggaaaggaga atcagtctgg gatcgatttg tacggatacc tggaaagaca tttaaaggaa
      241 cgaacggtga tttggcagta gaccactatc atcgttataa agaagatatt gctttaatga
      301 aagaacaagg tctgaaagct taccgcttct ctgtagcttg gacacggatt tttcctaatg
      361 gacgaggaga agtcaatcaa gctgggctcg ctttttatga gagattgatc gacgaattga
      421 ttgaaaacga tattgaacct atgttgactt tatatcattg ggatcttcca caagcattgc
      481 aagatgaata taatggttgg gaatcacgtc agatcattga agatttcact cattatgcag
      541 aaacattatt tgaggcattc cgcgggaaag tccattactg gattagtttg aatgaacaaa
      601 atatcttcac ttcattaggt tatttgttag cagcacatcc gccaggtgtg acagacccga
      661 aacgaatgta tgaagttaac catattgcta atttagcaaa tgcatctgtc atcaataaat
      721 ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc
      781 caattgacag taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca
      841 attattggat ggatgtctat atgtggggaa aatatccgat tgctgccatg aggttcttag
      901 aagaaaaagg atgggcacca acgattgaag ctggcgatgc agaattgcta gaatctgcta
      961 aaccagattt tcttgggatc aattactatc aaacggcgac gaatgcattc aatccattaa
     1021 acggagttgg tgctggaaaa atgaatacga caggaaaaaa aggttcatca gaagaaactg
     1081 gagttcctgg aatgtataaa aaagttgaga atccatttgt cgaacgaaca aattgggact
     1141 gggagattga cccagaagga ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca
     1201 tgccggtact gattacggaa aatggcttag gagagtatga caagttgaca gaagataaac
     1261 aaatccatga cgactatcga atcaattact tacaaagcca cgtcaaagcc atcaaagaag
     1321 caatttcgga tggagcagaa gtcctaggct attgcacctg gtcttataca gatttgctta
     1381 gctggctgaa cggttatcaa aagcgatatg gatttgtata tgttgatcaa gacgaaacac
     1441 aagaaggatc tttggaacga tataagaaag acagttatta ctggtaccaa aaactgattg
     1501 aagaaaatgg agagaacctt tagttcacat acttaaaaca agaaggtacg gctggatatg
     1561 gccgtgcctt ttcttgataa aatcatttag aaattaagaa aaactgtaaa ataagctgaa
     1621 aaatgaaaga aagcgtcagt tctttcaaac aaaactttag ttcccttact tttttttgta
     1681 taatgaaact tgatgaaatg agggataata aatggcacaa aagaaacggc ctactaaaaa
     1741 aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa tgctgacgat
     1801 tggtgttggg tttgccttga ttttatttgc tgtcttcggc tttttaaaat taggtttttt
     1861 aggaatattg attgcaaacg gctttcggat cattgcagga aatacttacc aaatcctctg
     1921 tcttttatta gccgtattag gattttggat tgtcattaag aatacagagt tttcaattgg
     1981 taaaaaccga cgctggtttg gcggcatact cttttatttc ggtatcttat tattgttaca
     2041 tgcccattta tttggcaaac tccatactgg ggaaccaaat atcatgggga cgacatggga
     2101 tttgttagcc agtgatataa aacaaagtca agtagacaat aacgttggtg gtgggatgat
     2161 tggagcaatc ctttatcatt ttacttactt cttgattgca cagcctggaa gttatttagc
     2221 agcagtgcta ttactggcag gaggagcatt cctgttcagt aatctggaag gctatcagct
     2281 gttaaatggt atccaatcaa ttggagaacg agtacaggaa ttattggaag gtgatcctgc
     2341 caagcaagct cgaaagcaag cggcaaagga agagagaatg aaacagcgcg cagaagcgaa
     2401 agaagcaagg cgcttagctg caaaagaagc tgcagaaaaa gaagctgcag aatatgagaa
     2461 gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat gaatggcagg aaccagaaaa
     2521 tacagagccg gagcagctaa gttttgttcc aatcgacagt tttcaagaaa atatccatcc
     2581 tgcgaatcta gagaaaccag taccagatac accaaaacag accaatacag cagaaggttt
     2641 ttcagatgag ttgccagagg atgacggtac ctcattagaa tttgagattg aagcagaaca
     2701 ggaaaatcaa gactatgaat taccgacagt tgatttgctt gattctatac caactgtgga
     2761 tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc gtattagaac aaactttcca
     2821 aagtttcggt gtcgatgcca aggtcgtcaa agccagctta ggaccatccg tcacaaaatt
     2881 cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat
     2941 cgcgcttgct ttagcggcga aagatgtccg gatggaagca ccgattccag gtaaatcact
     3001 gattggtatt gaagtaccaa atggaaagat cagtatggtt tcctttagag aaatcatcga
     3061 agcacaacca aaccacccag ataaattact agaagtacct ttaggacgtg atgtttctgg
     3121 acgggtacaa acggctgatc tttcgaagat gcctcactta ctcgtggctg gttcgactgg
     3181 tagtgggaag tcagtagcaa ttaatgggat tatcacaagt atattaatgc gtgctaagcc
     3241 ccatgaagta aaactgatga tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg
     3301 catacctcat ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc aagccttaca
     3361 aaaagtagta caagaaatgg aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa
     3421 tatttctggt tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc
     3481 aaccttacca ttcatcgtag tgatcgtaga tgaattagct gatttgatga tggtagccag
     3541 caatgaagtt gaagatgcga ttattcgttt agcgcaaatg gctcgggcag cggggattca
     3601 tatgatccta gctactcaac gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa
     3661 tgtaccttcc agaatggcat ttgcggtatc aagcggtaca gattctcgga cgatcattga
     3721 ttctaatggg gcagaaaagc tcttagggcg aggagatatg ctattcttgc caatgggtga
     3781 gaataaaccg atccgtgtgc aaggagcatt catttctgac cacgaagtag aacgggtcgt
     3841 tcaatttgtt acagatcagc aagaagctca ttatgaagag aaaatgatgc cgacagatga
     3901 agttgaaacg acaggtgctc cagaacagcc acaagatgag ctgtttgaag aagcaaaagc
     3961 acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc caaagaagat ttcggatcgg
     4021 ttacaaccga gcagcacgtt tagtagatga actggaagct catggagttg ttggcccgtc
     4081 agaaggaagc aagcctcgga aagtattcat tgagcaggaa gaaacatcag aaacgttgcc
     4141 ggacgataat gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat aaccaataaa
     4201 aagacaaggc atgatgatac caaaagaaaa aatggttttt ttccatcttt tctaatacgg
     4261 tatgatgtct tgtcttttta gtttttgtga gattaaagtt ctattcgatt tatttgtgaa
     4321 gtattgcatt aaggaaatga atcatgtata gtatgtagcg aaactttgag aaaaagaggg
     4381 aaaatacttt ggaaaaacag tccatttatg gcttgacgaa tgaagaatta atcaattggt
     4441 ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa
     4501 aacgtgtaag taatttcaca gaaatgacga atctatcgaa acaattgatc gaaaaattat
     4561 cggagcattt tatcatcaat cctctaaaac agatggtcgt ccaagaagct tcggatggta
     4621 cagtaaaata tttgtttgaa ctccctgata aaaacatgat tgagactgta ctgatgagac
     4681 aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt
     4741 tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac tgctggagaa attgttgcac
     4801 agatcatgat ggtccaacat tattttgatg agcgcaatct tggcgaacgc gtttcacatg
     4861 tcgttgtaat ggggatcggt gagccatttg ataattacga caatgtaatg gactttttgc
     4921 atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg gcacattaca gtttccacta
     4981 gtggattagc acacaaaatc aaggaattcg ctaataacgg cttgcaagtc aatctggcta
     5041 tttccttgca cgcgccgaat aatgaggtgc gaacatcgat catgcggatc aaccgaagtt
     5101 tcccaattga gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa
     5161 tcacgtttga atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat
     5221 tagcagatct attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc
     5281 cagtcagtga acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg
     5341 atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg
     5401 atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt
     5461 atcctactcg ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc
     5521 aacttaaata agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa
     5581 accctcatca agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta
     5641 gcaattattt aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt
     5701 tctacgtcaa gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta
     5761 atatcatttg ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga
     5821 ttcatgtcgg ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca
     5881 aacaaatcga actgctgatc catcatcaat aaggtcagta caccataact gacagatgct
     5941 ccgtgaaggt atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc
     6001 atagcagaat tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata
     6061 tccgaaacga cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct
     6121 tgcgtaccat aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta
     6181 tccgtgtagc ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa
     6241 gcatcgccaa tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca
     6301 atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg
     6361 ttatagatga cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg
     6421 aagactggtt tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca
     6481 ccgactgcaa acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata
     6541 tttccataag ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc
     6601 aaagctactt ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact
     6661 acttttgtcc catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg
     6721 gtataatttg gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac
     6781 gggttaaaat tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac
     6841 ctaagacagg ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat
     6901 cctaaaaacg aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt
     6961 agtatttcta ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga
     7021 ttattatatg aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag
     7081 ataatctata tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc
     7141 tttcctattt tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata
     7201 atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt
     7261 ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat
     7321 ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc
     7381 cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa
     7441 tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat
     7501 acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc
     7561 aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca
     7621 cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt
     7681 atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat
     7741 tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca
     7801 aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag
     7861 aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa aattacttct
     7921 tgaatttttg tgaaattcca acttatttcc gaagaagcta cttttggaac accgtgtacc
     7981 agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     8041 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
     8101 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
     8161 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
     8221 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
     8281 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
     8341 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
     8401 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
     8461 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
     8521 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
     8581 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
     8641 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
     8701 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
     8761 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
     8821 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
     8881 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
     8941 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
     9001 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
     9061 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     9121 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     9181 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     9241 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     9301 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     9361 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     9421 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     9481 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     9541 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     9601 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     9661 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     9721 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     9781 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     9841 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     9901 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     9961 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
    10021 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
    10081 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
    10141 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
    10201 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
    10261 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
    10321 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
    10381 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
    10441 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
    10501 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
    10561 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
    10621 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
    10681 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
    10741 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
    10801 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
    10861 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
    10921 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
    10981 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
    11041 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
    11101 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
    11161 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
    11221 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
    11281 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
    11341 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
    11401 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
    11461 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
    11521 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
    11581 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
    11641 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
    11701 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
    11761 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
    11821 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
    11881 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
    11941 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
    12001 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
    12061 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
    12121 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
    12181 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
    12241 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
    12301 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
    12361 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
    12421 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
    12481 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
    12541 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
    12601 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
    12661 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
    12721 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
    12781 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
    12841 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
    12901 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
    12961 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
    13021 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatactga
    13081 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
    13141 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
    13201 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
    13261 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
    13321 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
    13381 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
    13441 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
    13501 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
    13561 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
    13621 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
    13681 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
    13741 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
    13801 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
    13861 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
    13921 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
    13981 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
    14041 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
    14101 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
    14161 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
    14221 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
    14281 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
    14341 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
    14401 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
    14461 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
    14521 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
    14581 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
    14641 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
    14701 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
    14761 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
    14821 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
    14881 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
    14941 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
    15001 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
    15061 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
    15121 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
    15181 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
    15241 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
    15301 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
    15361 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
    15421 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
    15481 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
    15541 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
    15601 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
    15661 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
    15721 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
    15781 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
    15841 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
    15901 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
    15961 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
    16021 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
    16081 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
    16141 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
    16201 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
    16261 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
    16321 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
    16381 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
    16441 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
    16501 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
    16561 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
    16621 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
    16681 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
    16741 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
    16801 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
    16861 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
    16921 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
    16981 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
    17041 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
    17101 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
    17161 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
    17221 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
    17281 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
    17341 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
    17401 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
    17461 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
    17521 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
    17581 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
    17641 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
    17701 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
    17761 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
    17821 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
    17881 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
    17941 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
    18001 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
    18061 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    18121 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    18181 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    18241 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    18301 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    18361 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    18421 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    18481 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    18541 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    18601 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    18661 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    18721 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    18781 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    18841 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    18901 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    18961 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    19021 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    19081 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    19141 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    19201 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    19261 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    19321 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    19381 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    19441 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    19501 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    19561 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    19621 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    19681 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    19741 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    19801 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    19861 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    19921 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    19981 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    20041 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    20101 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    20161 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    20221 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    20281 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    20341 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    20401 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    20461 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    20521 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    20581 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    20641 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    20701 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    20761 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    20821 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    20881 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    20941 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    21001 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    21061 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    21121 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    21181 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    21241 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    21301 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    21361 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    21421 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    21481 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    21541 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    21601 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    21661 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    21721 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    21781 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    21841 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    21901 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    21961 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    22021 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    22081 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    22141 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    22201 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    22261 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    22321 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    22381 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    22441 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    22501 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    22561 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    22621 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    22681 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    22741 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    22801 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    22861 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    22921 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    22981 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    23041 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    23101 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    23161 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    23221 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    23281 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    23341 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    23401 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    23461 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    23521 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    23581 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    23641 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    23701 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    23761 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    23821 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    23881 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    23941 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    24001 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    24061 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    24121 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    24181 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    24241 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    24301 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    24361 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    24421 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    24481 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    24541 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    24601 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    24661 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    24721 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    24781 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    24841 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    24901 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    24961 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    25021 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    25081 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    25141 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    25201 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    25261 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    25321 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    25381 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    25441 ctgcaccaac at
//
LOCUS       DASJPH010000041        25362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJPH010000041 DASJPH010000000
VERSION     DASJPH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.041"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U3995_001436"
     CDS             complement(104..1336)
                     /locus_tag="U3995_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1796765.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U3995_001437"
     CDS             1591..2160
                     /locus_tag="U3995_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1796766.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U3995_001438"
     CDS             complement(2338..2778)
                     /locus_tag="U3995_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1796767.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U3995_001439"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U3995_001439"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1796768.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U3995_001440"
     CDS             3732..4655
                     /locus_tag="U3995_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1796769.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U3995_001441"
     CDS             4731..5870
                     /locus_tag="U3995_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1796770.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U3995_001442"
     CDS             5863..6663
                     /locus_tag="U3995_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1796771.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U3995_001443"
     CDS             6663..7490
                     /locus_tag="U3995_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1796772.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U3995_001444"
     CDS             7468..8202
                     /locus_tag="U3995_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1796773.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U3995_001445"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U3995_001445"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1796774.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U3995_001446"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U3995_001446"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1796775.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U3995_001447"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U3995_001447"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1796776.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U3995_001448"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U3995_001448"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1796777.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U3995_001449"
     CDS             11787..13820
                     /locus_tag="U3995_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796778.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U3995_001450"
     CDS             13864..15144
                     /locus_tag="U3995_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1796779.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U3995_001451"
     CDS             15354..16160
                     /locus_tag="U3995_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796780.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="U3995_001452"
     CDS             16172..17392
                     /locus_tag="U3995_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796781.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="U3995_001453"
     CDS             17382..18968
                     /locus_tag="U3995_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1796782.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="U3995_001454"
     CDS             19007..21145
                     /locus_tag="U3995_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1796783.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="U3995_001455"
     CDS             complement(21513..22496)
                     /locus_tag="U3995_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1796784.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="U3995_001456"
     CDS             22874..24265
                     /locus_tag="U3995_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1796785.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="U3995_001457"
     CDS             24281..25321
                     /locus_tag="U3995_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1796786.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
BASE COUNT         8046 a         4298 c         5176 g         7842 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt ca
//
LOCUS       DASJPH010000042        24551 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJPH010000042 DASJPH010000000
VERSION     DASJPH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.042"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U3995_001458"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U3995_001458"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1796787.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="U3995_001459"
     CDS             complement(937..1518)
                     /locus_tag="U3995_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1796788.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U3995_001460"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U3995_001460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1796789.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="U3995_001461"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="U3995_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1796790.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="U3995_001462"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="U3995_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1796791.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="U3995_001463"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="U3995_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1796792.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="U3995_001464"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="U3995_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1796793.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="U3995_001465"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="U3995_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1796794.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="U3995_001466"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="U3995_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1796795.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="U3995_001467"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="U3995_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1796796.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="U3995_001468"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="U3995_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1796797.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="U3995_001469"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="U3995_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1796798.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="U3995_001470"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="U3995_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1796799.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="U3995_001471"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="U3995_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1796800.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="U3995_001472"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="U3995_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1796801.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="U3995_001473"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="U3995_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1796802.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="U3995_001474"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="U3995_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1796803.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="U3995_001475"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="U3995_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1796804.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="U3995_001476"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="U3995_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1796805.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="U3995_001477"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="U3995_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1796806.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="U3995_001478"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="U3995_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1796807.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="U3995_001479"
     CDS             13972..14157
                     /locus_tag="U3995_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1796808.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="U3995_001480"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="U3995_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1796809.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="U3995_001481"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="U3995_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1796810.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="U3995_001482"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="U3995_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1796811.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="U3995_001483"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="U3995_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1796812.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="U3995_001484"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="U3995_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1796813.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="U3995_001485"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="U3995_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1796814.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="U3995_001486"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="U3995_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1796815.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="U3995_001487"
     CDS             18232..18879
                     /locus_tag="U3995_001487"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1796816.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="U3995_001488"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="U3995_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1796817.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="U3995_001489"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="U3995_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1796818.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="U3995_001490"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="U3995_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1796819.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="U3995_001491"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="U3995_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1796820.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="U3995_001492"
     CDS             20317..21255
                     /locus_tag="U3995_001492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1796821.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="U3995_001493"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="U3995_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1796822.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="U3995_001494"
     CDS             21935..22774
                     /locus_tag="U3995_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1796823.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="U3995_001495"
     CDS             22750..23619
                     /locus_tag="U3995_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1796824.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="U3995_001496"
     CDS             23609..24406
                     /locus_tag="U3995_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1796825.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7953 a         4548 c         5279 g         6771 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa a
//
LOCUS       DASJPH010000043        24336 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJPH010000043 DASJPH010000000
VERSION     DASJPH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.043"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..2478)
                     /locus_tag="U3995_001497"
     CDS             complement(133..2478)
                     /locus_tag="U3995_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1796826.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="U3995_001498"
     CDS             2757..3659
                     /locus_tag="U3995_001498"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1796827.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="U3995_001499"
     CDS             3870..5645
                     /locus_tag="U3995_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1796828.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="U3995_001500"
     CDS             complement(5711..7297)
                     /locus_tag="U3995_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1796829.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="U3995_001501"
     CDS             7548..7772
                     /locus_tag="U3995_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1796830.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="U3995_001502"
     CDS             7914..9665
                     /locus_tag="U3995_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1796831.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="U3995_001503"
     CDS             9665..11449
                     /locus_tag="U3995_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796832.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="U3995_001504"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="U3995_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="U3995_001505"
     CDS             12733..14619
                     /locus_tag="U3995_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1796833.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="U3995_001506"
     CDS             complement(14660..15562)
                     /locus_tag="U3995_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1796834.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="U3995_001507"
     CDS             15792..17195
                     /locus_tag="U3995_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1796835.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17192..18628
                     /locus_tag="U3995_001508"
     CDS             17192..18628
                     /locus_tag="U3995_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1796836.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="U3995_001509"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="U3995_001509"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1796837.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="U3995_001510"
     CDS             19912..21639
                     /locus_tag="U3995_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1796838.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="U3995_001511"
     CDS             21655..22005
                     /locus_tag="U3995_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1796839.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="U3995_001512"
     CDS             complement(22099..22959)
                     /locus_tag="U3995_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1796840.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="U3995_001513"
     CDS             23051..23479
                     /locus_tag="U3995_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1796841.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /locus_tag="U3995_001514"
     CDS             23641..23817
                     /locus_tag="U3995_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1796842.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="U3995_001515"
     CDS             23844..24290
                     /locus_tag="U3995_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1796843.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4352 c         5187 g         7089 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaat
//
LOCUS       DASJPH010000044        23830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJPH010000044 DASJPH010000000
VERSION     DASJPH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.044"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..790)
                     /locus_tag="U3995_001516"
     CDS             complement(56..790)
                     /locus_tag="U3995_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1796844.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="U3995_001517"
     CDS             1117..1527
                     /locus_tag="U3995_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1796845.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="U3995_001518"
     CDS             1520..2221
                     /locus_tag="U3995_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1796846.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="U3995_001519"
     CDS             2221..3807
                     /locus_tag="U3995_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1796847.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="U3995_001520"
     CDS             3826..4047
                     /locus_tag="U3995_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1796848.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="U3995_001521"
     CDS             4044..4805
                     /locus_tag="U3995_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1796849.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="U3995_001522"
     CDS             5185..5694
                     /locus_tag="U3995_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1796850.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="U3995_001523"
     CDS             complement(5769..6308)
                     /locus_tag="U3995_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1796851.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U3995_001524"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U3995_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1796852.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="U3995_001525"
     CDS             7321..8172
                     /locus_tag="U3995_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1796853.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="U3995_001526"
     CDS             8200..8835
                     /locus_tag="U3995_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1796854.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="U3995_001527"
     CDS             8855..9628
                     /locus_tag="U3995_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1796855.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="U3995_001528"
     CDS             10036..10833
                     /locus_tag="U3995_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1796856.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="U3995_001529"
     CDS             10811..11224
                     /locus_tag="U3995_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1796857.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="U3995_001530"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="U3995_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1796858.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="U3995_001531"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="U3995_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1796859.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="U3995_001532"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="U3995_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1796860.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U3995_001533"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U3995_001533"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1796861.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="U3995_001534"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="U3995_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1796862.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="U3995_001535"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="U3995_001535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1796863.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="U3995_001536"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="U3995_001536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1796864.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="U3995_001537"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="U3995_001537"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1796865.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="U3995_001538"
     CDS             21908..22486
                     /locus_tag="U3995_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1796866.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="U3995_001539"
     CDS             22538..23020
                     /locus_tag="U3995_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1796867.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="U3995_001540"
     CDS             23137..23511
                     /locus_tag="U3995_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796868.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4286 c         5116 g         6644 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttatttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt
//
LOCUS       DASJPH010000045        23642 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJPH010000045 DASJPH010000000
VERSION     DASJPH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.045"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="U3995_001541"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="U3995_001541"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1796869.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="U3995_001542"
     CDS             complement(1171..1866)
                     /locus_tag="U3995_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1796870.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="U3995_001543"
     CDS             complement(1879..2595)
                     /locus_tag="U3995_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1796871.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="U3995_001544"
     CDS             2925..3533
                     /locus_tag="U3995_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1796872.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="U3995_001545"
     CDS             3858..4181
                     /locus_tag="U3995_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1796873.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="U3995_001546"
     CDS             4363..5733
                     /locus_tag="U3995_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1796874.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="U3995_001547"
     CDS             complement(5863..7164)
                     /locus_tag="U3995_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1796875.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9476)
                     /locus_tag="U3995_001548"
     CDS             complement(7518..9476)
                     /locus_tag="U3995_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1796876.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="U3995_001549"
     CDS             complement(9653..9919)
                     /locus_tag="U3995_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1796877.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="U3995_001550"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="U3995_001550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1796878.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="U3995_001551"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="U3995_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1796879.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /locus_tag="U3995_001552"
     CDS             13263..13826
                     /locus_tag="U3995_001552"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1796880.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="U3995_001553"
     CDS             complement(14079..14600)
                     /locus_tag="U3995_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1796881.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="U3995_001554"
     CDS             complement(14603..15445)
                     /locus_tag="U3995_001554"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1796882.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="U3995_001555"
     CDS             complement(15438..16163)
                     /locus_tag="U3995_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1796883.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="U3995_001556"
     CDS             16339..16635
                     /locus_tag="U3995_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796884.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17203..18795
                     /locus_tag="U3995_001557"
     CDS             17203..18795
                     /locus_tag="U3995_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1796885.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="U3995_001558"
     CDS             18808..19548
                     /locus_tag="U3995_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1796886.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="U3995_001559"
     CDS             19716..21065
                     /locus_tag="U3995_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1796887.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="U3995_001560"
     CDS             complement(21193..21783)
                     /locus_tag="U3995_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1796888.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="U3995_001561"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="U3995_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1796889.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DASJPH010000046        23210 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJPH010000046 DASJPH010000000
VERSION     DASJPH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.046"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..329)
                     /locus_tag="U3995_001562"
     CDS             complement(<1..329)
                     /locus_tag="U3995_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796890.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="U3995_001563"
     CDS             complement(343..600)
                     /locus_tag="U3995_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796891.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="U3995_001564"
     CDS             complement(795..1337)
                     /locus_tag="U3995_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796892.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="U3995_001565"
     CDS             complement(1358..2449)
                     /locus_tag="U3995_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1796893.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="U3995_001566"
     CDS             complement(2457..4667)
                     /locus_tag="U3995_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796894.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="U3995_001567"
     CDS             complement(4683..7211)
                     /locus_tag="U3995_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1796895.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="U3995_001568"
     CDS             complement(7263..7673)
                     /locus_tag="U3995_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796896.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="U3995_001569"
     CDS             complement(7686..7904)
                     /locus_tag="U3995_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796897.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="U3995_001570"
     CDS             complement(7901..8893)
                     /locus_tag="U3995_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1796898.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="U3995_001571"
     CDS             complement(8972..9883)
                     /locus_tag="U3995_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1796899.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="U3995_001572"
     CDS             complement(9902..10246)
                     /locus_tag="U3995_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796900.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10822)
                     /locus_tag="U3995_001573"
                     /pseudo
     CDS             complement(10266..10822)
                     /locus_tag="U3995_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10903..11403)
                     /locus_tag="U3995_001574"
     CDS             complement(10903..11403)
                     /locus_tag="U3995_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796901.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="U3995_001575"
     CDS             complement(11404..11949)
                     /locus_tag="U3995_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796902.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="U3995_001576"
     CDS             complement(11946..13202)
                     /locus_tag="U3995_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1796903.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="U3995_001577"
     CDS             complement(13563..13874)
                     /locus_tag="U3995_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796904.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15207)
                     /locus_tag="U3995_001578"
     CDS             complement(13897..15207)
                     /locus_tag="U3995_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN1796905.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="U3995_001579"
     CDS             complement(15821..16273)
                     /locus_tag="U3995_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796906.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="U3995_001580"
     CDS             complement(16275..16640)
                     /locus_tag="U3995_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796907.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="U3995_001581"
     CDS             complement(16739..21022)
                     /locus_tag="U3995_001581"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN1796908.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="U3995_001582"
     CDS             complement(21169..21408)
                     /locus_tag="U3995_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796909.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21770)
                     /locus_tag="U3995_001583"
     CDS             complement(21498..21770)
                     /locus_tag="U3995_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796910.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21853..22104)
                     /locus_tag="U3995_001584"
     CDS             complement(21853..22104)
                     /locus_tag="U3995_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796911.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..>23210
                     /locus_tag="U3995_001585"
     CDS             22267..>23210
                     /locus_tag="U3995_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1796912.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDD"
BASE COUNT         6854 a         4991 c         3504 g         7861 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt
//
LOCUS       DASJPH010000047        22711 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJPH010000047 DASJPH010000000
VERSION     DASJPH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.047"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..1942)
                     /locus_tag="U3995_001586"
     CDS             complement(11..1942)
                     /locus_tag="U3995_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1796913.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="U3995_001587"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="U3995_001587"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1796914.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="U3995_001588"
     CDS             complement(2871..3623)
                     /locus_tag="U3995_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1796915.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="U3995_001589"
     CDS             complement(3782..3991)
                     /locus_tag="U3995_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796916.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="U3995_001590"
     CDS             complement(4002..5330)
                     /locus_tag="U3995_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796917.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="U3995_001591"
     CDS             5466..5621
                     /locus_tag="U3995_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796918.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /locus_tag="U3995_001592"
     CDS             complement(5695..6282)
                     /locus_tag="U3995_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1796919.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="U3995_001593"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="U3995_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1796920.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /locus_tag="U3995_001594"
     CDS             complement(7862..9151)
                     /locus_tag="U3995_001594"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1796921.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="U3995_001595"
     CDS             complement(9305..10237)
                     /locus_tag="U3995_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796922.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="U3995_001596"
     CDS             complement(10394..11053)
                     /locus_tag="U3995_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1796923.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="U3995_001597"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="U3995_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1796924.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="U3995_001598"
     CDS             complement(12094..12384)
                     /locus_tag="U3995_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796925.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="U3995_001599"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="U3995_001599"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1796926.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /locus_tag="U3995_001600"
     CDS             complement(14670..16025)
                     /locus_tag="U3995_001600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1796927.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="U3995_001601"
     CDS             complement(16049..17218)
                     /locus_tag="U3995_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796928.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /locus_tag="U3995_001602"
     CDS             complement(17215..18102)
                     /locus_tag="U3995_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1796929.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="U3995_001603"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="U3995_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1796930.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="U3995_001604"
     CDS             22182..22703
                     /locus_tag="U3995_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1796931.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6325 a         4931 c         4036 g         7419 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata a
//
LOCUS       DASJPH010000048        21180 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJPH010000048 DASJPH010000000
VERSION     DASJPH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.048"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1078
                     /locus_tag="U3995_001605"
     CDS             <1..1078
                     /locus_tag="U3995_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1796932.1"
                     /translation="DKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQ
                     EDVIVSVTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANV
                     IYRPVHELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIK
                     QTRMTEFEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPL
                     TDVPVVGAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQT
                     SRAKRGVTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGS
                     FAMDEKKGGEVIEVIDAPEIFIES"
     gene            1355..3586
                     /gene="pflB"
                     /locus_tag="U3995_001606"
     CDS             1355..3586
                     /gene="pflB"
                     /locus_tag="U3995_001606"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1796933.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            3830..4591
                     /gene="pflA"
                     /locus_tag="U3995_001607"
     CDS             3830..4591
                     /gene="pflA"
                     /locus_tag="U3995_001607"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1796934.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            4676..5602
                     /locus_tag="U3995_001608"
     CDS             4676..5602
                     /locus_tag="U3995_001608"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1796935.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            5784..6677
                     /locus_tag="U3995_001609"
     CDS             5784..6677
                     /locus_tag="U3995_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1796936.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            6811..6936
                     /locus_tag="U3995_001610"
     CDS             6811..6936
                     /locus_tag="U3995_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796937.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(7070..7702)
                     /locus_tag="U3995_001611"
     CDS             complement(7070..7702)
                     /locus_tag="U3995_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796938.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(7850..8392)
                     /locus_tag="U3995_001612"
     CDS             complement(7850..8392)
                     /locus_tag="U3995_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1796939.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            8527..8916
                     /locus_tag="U3995_001613"
     CDS             8527..8916
                     /locus_tag="U3995_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796940.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            8920..9684
                     /locus_tag="U3995_001614"
     CDS             8920..9684
                     /locus_tag="U3995_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1796941.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            9813..10760
                     /locus_tag="U3995_001615"
     CDS             9813..10760
                     /locus_tag="U3995_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1796942.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            10890..11630
                     /locus_tag="U3995_001616"
     CDS             10890..11630
                     /locus_tag="U3995_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796943.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(11921..12589)
                     /locus_tag="U3995_001617"
     CDS             complement(11921..12589)
                     /locus_tag="U3995_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1796944.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            12723..13010
                     /locus_tag="U3995_001618"
     CDS             12723..13010
                     /locus_tag="U3995_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796945.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            13426..14964
                     /locus_tag="U3995_001619"
     CDS             13426..14964
                     /locus_tag="U3995_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1796946.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            14961..15926
                     /locus_tag="U3995_001620"
     CDS             14961..15926
                     /locus_tag="U3995_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796947.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            15913..17235
                     /locus_tag="U3995_001621"
     CDS             15913..17235
                     /locus_tag="U3995_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1796948.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      17298..17402
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17476..18219
                     /locus_tag="U3995_001622"
     CDS             17476..18219
                     /locus_tag="U3995_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1796949.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            18234..19226
                     /locus_tag="U3995_001623"
     CDS             18234..19226
                     /locus_tag="U3995_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1796950.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            19238..19975
                     /locus_tag="U3995_001624"
     CDS             19238..19975
                     /locus_tag="U3995_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1796951.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            19965..20687
                     /locus_tag="U3995_001625"
     CDS             19965..20687
                     /locus_tag="U3995_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1796952.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(20771..>21180)
                     /locus_tag="U3995_001626"
     CDS             complement(20771..>21180)
                     /locus_tag="U3995_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1796953.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         7203 a         3604 c         4355 g         6018 t
ORIGIN      
        1 tgacaaagag cttttctctg ttatgaaaaa agaattgcga gaagtgaaaa aaaattattc
       61 ttccgctcgt ctaacgacaa tcgaagacga aatcgaagaa atcaaaattg atacgcaagt
      121 cttagtggca caagaagacg taattgtcag tgtgacgcga gaaggctatg tgaagcgaac
      181 aagccttcgt tcttatagtg cttccaaacc agaagaaatc gggatgcgag aaggcgacta
      241 cttgctatac tcaggtgaat tgagtacatt ggaccatgtt cttctgatca caaataaagc
      301 aaatgtgatc taccgccctg tccatgaact acccgatttg aaatggaaag atgctggtga
      361 gcacatttct caaacaatct ccaatctgtc tgtagacgaa tcgattttag ctgttttccc
      421 atatcaaaaa attcaagcag agaaaacatt tgtattcatc agcaagaatg gattgatcaa
      481 gcaaacacga atgacagagt tcgaaccatg gcgcacctat aagagccgtc cgctcagctg
      541 tatgaagcta aaggcggaag aagatgaact gattgctgtg tatctgaaag aagatcagcc
      601 aggcttagat gttttcttag taacgcatca aggaatgggt ttgcgctatc ctttaacaga
      661 tgttcccgtt gttggagcaa aggcagctgg agtgaagtct atgaatttga aagatggaga
      721 taggattatc aacgggctgc ttgtgttagc agaaggtgat acgccgatcg taatcgttac
      781 tcagcgagga gctgttaaac gcatgcttgc tcaagaaatc agccaaactt cacgtgcgaa
      841 gcggggagtg acggtcatgc gtgagctgaa aaaaaatccg caccgtatta tctatatgtc
      901 ggaaggaaga tcaaaagaaa taaccttgat gaatcaaaaa gggcagcagt taacgattga
      961 cccaactgat ttccctatag gtgatcgaac atcgaatgga tcatttgcga tggatgagaa
     1021 aaaaggtgga gaagtcatcg aagttatcga tgctcctgag attttcatcg agtcgtaact
     1081 gttgtagtat tttaatgaag agacgccatc tgtattaata caggtggcgt ctctttttca
     1141 ttcattttta ataaatcctt atacgtttgc aaaaaaagat gaaagtgttc acaatgctta
     1201 aatgttggta tgtcaacgtt gatttaaata tatgtaaata aaacataaaa attgttaaat
     1261 aaaaaacaag aaaacccttg caaagatagg gtgctgtgtt atactaatca cgtaaaaact
     1321 gttacataaa acagaaattt aggaggtcca tacaatggag caatggaatg gatttaaagg
     1381 taccaaatgg aaagaaggcg tagacactag agactttatt caaaataact acactgaata
     1441 tcgcggtgat gacagctttt tggaaggccc aacagaagca acagaaaaat tatgggatga
     1501 attgcaaaag ttaaatgata tccaattcga gcgtaacggt gtctatgata tggacacaga
     1561 aatcgtatca actattactt cacacgatgc aggctatttg gataaagatt tggaaaaaat
     1621 cgtcggtcta cagacagaca aacctttgaa acaagcattc atgccgttcg gcggaatcaa
     1681 tatggctaac aatgcgctaa aaagcaatgg ctatacacca aacgatgaat tgacacatat
     1741 tttcactgac tggcgcaaaa cacataatca aggtgtattt gacgcttata ctgctgaaat
     1801 gcgtgctgct cgtaaaaaca aaatcattac agggcttcca gacgcttacg gtcgcggacg
     1861 tatcatcggt gactatcgtc gtgtggcatt gtatggaatc gactatttga tggaacaaaa
     1921 gaaaaaagat catgatatga ctggttatca tacaatgagt gaagaagtga ttcgtttacg
     1981 tgaagaaatc actgaacaat atcgtgcatt agcgcaaatg aaagaaatgg ctgcgaaata
     2041 tggctatgat atttctaaac cagctgcaaa tgcaaaagaa gctgtccaat ggctatactt
     2101 cggatattta gcagcaatca aatcacaaaa tggtgctgcg atgtcaatcg gacgtatctc
     2161 agcattcctt gatatctata tccaacgtga tttggataat ggagtaatca cagaaaaaga
     2221 agcacaagaa ttgatcgacc acttagtaat gaaattacgt atggtcaaat tcgcgcgtac
     2281 acctgaatat aaccaattat tctcaggatt ccctatctgg gctactttat ccattgcagg
     2341 tatgggatta gatggtcgtt cattagttac aaagaatgat ttccgtctgc ttcatacatt
     2401 aacaaatatg ggaccttcac cagaaccaaa cttgactgtt ttgtattctg aacaccttcc
     2461 agaaggattc agaacatatg ctagcagaat cgctatcgaa agttcttcga ttcaattcga
     2521 aaatgataat cagcttcgtg aacaatgggg ttctgatgat tgtgcgattg cttgctgtgt
     2581 atcaggaaca gttgtcggaa aagacatgca attctttggc gctcgtgcta acttggctaa
     2641 aacactatta tatgccatca atggcggagt cgatgaagtg actggcgccc aagttggacc
     2701 agaatatcgt ccaatcacat cagaaatact agactttgaa gaagtgaaac ataacttcat
     2761 gaatatgatg gattggttag cagaactata tgtcaatacg ttgaacatca ttcattatat
     2821 gcatgataaa tattcttatg aagcagctca gctggctttg atggattctg acttgcaacg
     2881 cacatttgct acaggtattg ctgggatctc tcatgcggca gacagcttgt cagcgatcaa
     2941 atatgcaaaa gtaaaagcaa tccgtaatga aaaaggaata gcagttgatt ttgaagtgga
     3001 tggcgacttc cctaaatacg gaaatgatga tgaccgtgca gatgaattag tttcatggat
     3061 cttggaatac ttcatgggac aaatcaaacg tcataaaact taccgcaact caaaaccaac
     3121 aacttcatta ttgacgatta cttcaaatgt tgtttacggt aaaaatacag gaaatactcc
     3181 tgatggccgt gaagctggaa aaccacttgc gccaggagca aatccaagtt acaatgcgga
     3241 acaaagtggg ttattagcta gcctgaactc aacagctaaa atgaattacg gctgtgcatt
     3301 ggatggaatt tcaaataccc aaacaatcaa tccttcaggt cttggaaaag aacaagatac
     3361 aagaatcgaa aacttgcgta atgtcttaga tggatacttc tcaaaaggag cttatcattt
     3421 gaatgtcaat gtattcagta atgatttatt gcgtgatgca atggaaaatc caatgaaata
     3481 tccgaacttg acgatccggg tatctggtta tgcagttaaa ttccgtgact tgacaagaga
     3541 acaacaatta gatgtcttga tgagaacttc acacgacaga atgtaaaaag ttcgtgaaaa
     3601 aagtttttcg acttagcgtc gaaaggacgt tgaatagcca tcgcatcaaa atcgaataat
     3661 cagtatgctt atttgaaaga aacgatttac aggggcagga cttgtatgtt ccgtccctgt
     3721 aaattttaca cataacgaag cggcaattgc gaaaaatttt gttcagaaag cttcgcttcg
     3781 acagctaact tgtacttaaa atgaaatcaa agaaaagatg tgaacttaaa tggaagaaaa
     3841 aacaatcggc tatgttcatt ctatcgaaac tttcggttca gtagacggac ctggactgcg
     3901 ctttgtcgtg tttatgcaag gatgccgcat gcgttgccaa ttttgtcaca atccagatac
     3961 ttggaatatt ggcggaggga aagaatacac agcagatgaa cttttagata aagcagaacg
     4021 cttcagacct tactggggag ataaaggtgg tattactgtt agtggtggag aacctctatt
     4081 gcagatcgat tttttgatcg aactatttaa aaaagcaaaa gagcggaaga tgcatactac
     4141 attggataca tgcggaaaac cttttacgta tgaagaacct ttcttttcac gttttcaaga
     4201 attgatgaag tatacagatt tactgttatt tgacatcaaa cacatcgata atgaagagca
     4261 taaaaaacta acgcattggg acaatgaaaa tattttagaa atggctcaat atttgtcaaa
     4321 aatcaacaaa ccggtttgga ttcgccatgt actagttcct gagcgaagtg attatgatga
     4381 atatttgatc cgactagaca actttattca aaccttgtca aatgtagaca gagtagagat
     4441 tcttccttat catacgatgg gaaaatacaa gtgggaaaca cttggtttga aatatccatt
     4501 agaagggatc gaaccaccta caaaagaacg tgtcgaaaat gctaaacgat tgcttcatac
     4561 agaagagtat acaaaatatt taacgcgata ggtcgcatcg gaaagaagat aagtattgct
     4621 tctttccgtt tttttgctat tattaaatag gagaaatcac aaaggagcga atgacatgtc
     4681 aaaaatttta gtttttggtc accaaaatcc agatacagat gcaatcgggg cagccattgc
     4741 gttttcacac ttgcaaaatg aattaggtaa agaaacagaa gcagttgctc taggtacacc
     4801 taatgaagaa acacaatacg cattagacca ctttcagcta tctgcaccac gagtagtaga
     4861 gtcagctaaa gcagaaaatg cagatgtcat gcttgtggat cataatgaat tccaacaaag
     4921 tatttcagat atcagcgaag taaatatttt agctgtagta gaccaccatc gtattgcaaa
     4981 ctttgaaaca gctaatccat tgtactatcg tgcagaacca gttggatgta caagtacgat
     5041 tattttaaaa ctatataaag aaaaccaagt aacaattcct aaagaaatcg caggaatcat
     5101 gctctcagct atcatttcag atacattgtt gttcaaatca ccaacatgta cgcctgaaga
     5161 tgaagcagct gcaaaagaac ttgcagcaat cgctgaagtc gatctaaaca cttatggact
     5221 tgaaatgttg aaagcaggaa caaatctaag tgataaatca gctgcggttc ttcttgattt
     5281 ggatgcaaaa agtttcccaa tgggcagttc caacgttcgt atcgctcaag tcaatacagt
     5341 ggaccttaat gaagtgatgg atcgacaagc agagctagaa gcagcaatgg aagatgaaat
     5401 gaaagcaaat ggctacgatc tgttcgtatt agttgtcaca aacatcttag acagtgattc
     5461 agaattactt gttgctggta aaggaaaaga aaaagtagaa gaagctttcc aaacaacttt
     5521 gaccaacaac cgtgctttgc ttaaaggtgt cgtttcacgt aaaaaacaag tcgtacctca
     5581 attgacggct gtatttagct aatcaaatgg tagtatgaag cattcaaaat gaatggataa
     5641 gcgattgata taaaaagaaa catagaacat actaaaggcc gaagaaatgg aggagaataa
     5701 cagtcaatta tgactcttgt tcttcctcct ttttacgtaa aagcctttta agtaaaataa
     5761 aacggataga aaagagaaca tatatgaact attattcaaa aaataaggaa aacttttatc
     5821 aaaaactgac cgatgatccg cttttttctt cattgaccga ttatctatat gaacatcgag
     5881 aacaagaaac gattctaaga gaattgaaaa aagaattttc gcaaaataaa ttttcccatt
     5941 ttcttgattt attgatcgat gcaggattaa tcaagaggga agaacgaaga tatcatttga
     6001 actttccaat atttgatcct aaagattact tgcaacaagc cacttcggct gctgaaacca
     6061 tagctgaaca attgaagaga ctctctgttg atgaacaaaa attagcgatg ggagaagtta
     6121 tttgggcgta ttgtttcgaa gatgaacgga aagaagcgta tttttatgga gtaagaaaca
     6181 gccgagagac agaattgctg aggacaactg caggaaatga gaagtaccgt tttatcacac
     6241 tttcctctat agaacacttc ccgcttactt tggctaatta cttttttgta cagaagaatc
     6301 agcttcctgt aacgaaggca ttcaaagaat tagccgaact tattggagac gtaaatgaat
     6361 cgtacttttt tgatcaaata gaagttatcg ttgacaggat acgtaagaat aaatataaaa
     6421 atcgccggcc tagtattttc catcagtctc tacttgtgac gaatactata aaggaagaag
     6481 aatcattcac attagaatta cctattgtcg aaaaaaacaa ttttgagatt gaattaccaa
     6541 cattggatcc ttcattgaca atggaggaaa tagcctttct taaacgacaa atcttttctg
     6601 agctgagcaa gaaatttata ccgcatgcat tttcttatat caaagaatat ggaacagttt
     6661 tagtgagtaa aacctaagag agcagtaagc aaaaattata ctgactcttg caggaggagc
     6721 ctttctcttc ctttatggtt aaaagaatta tttgagtatt ttgcctatct accacctatt
     6781 agattcacta catggtctgg aaacaaagga atgcaaaaaa atggtgagaa atgtggaatg
     6841 acaaaggaag tagtgataag aaaagttaga tttttaaata atcaatatta tgattcagtc
     6901 aagtatggca ttttatggga agaactagct gattagatta ggaagtccat ttgataatcc
     6961 agaatcacta agaaacgaat ggcgagtcat taataattta tccaatcttg ggttattctt
     7021 ttagattgaa gtgtttaaaa ctaaaaaagc atttatgaag tcgtaatagt caggcttcaa
     7081 aatgcttttt attttgtttg ttttttcttt gccacataaa tacacctaga ctgacgatga
     7141 agagcatatc caacagattt cctgcgactt ggatcgtcga agggttggtc agtggcatta
     7201 aagcaaggac aaaaaaggaa aacaagctgt aaataaaaca ggtgacagga actaatcgaa
     7261 agaagagcgg acatttcaat tggctaaaaa tgagtgaaaa aataaaataa gtaaaaatgg
     7321 ataaaatcac gactaaacca ttcatgataa agaagaaaag cgtattgtga ttaaacgaat
     7381 aaaaaagaat tgctaaaaaa gaaaacgttt taaatgcaat caatacgctt gaagcttcgt
     7441 ttaacgtacc aggatgtcga tgaaaaaaat aggcaccgaa ataaacagaa aacagtaagc
     7501 cgcaaagaga agacaaaatc aaaaaaaacg gattttcttc ggcttctgtg atatcagaat
     7561 gatccaaaag caaccagcct aatgcactag aaggcgcatg tgaggtctta aggtatgaat
     7621 aacatagaaa aaataagatc gtcaaagttg caaaaaaaga gcctaaatgt aaaaaattag
     7681 tggttctacg ttgaatttcc ataatatctc caaattaaat tgataagtat ataaagtata
     7741 ataacaaatt gatgagtata tgtcgatgtt ttttaatcaa aataataaat aaaaactgtc
     7801 taacatttaa aatttaattt agatattaga cagtttttaa gtttgtgagt tattttcctt
     7861 gtcgttttct cactttttta gtccagaaac cacctgaaat atattttggc tcataagaaa
     7921 tgataaaagc acgctcttcg atttctttga tcaggcgata cagttcacgt tcgctttttc
     7981 taggagaaag tatttcgaga actagccgct caccttccag ccccattgca gaagtctgtg
     8041 tcacaccata accgtgttct ctcagtatac ccggcaaact tttatcttct gcgctatttg
     8101 ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa tttgtcttca atcaaaattc
     8161 cgacactgat acctactgcg taacctaacg catagacaaa taaattcagt ggattatcta
     8221 aacgatctaa aacaagggat aagccgacga tataaatcgt gatctcgacc atactgacaa
     8281 gtggagcaat cagtcgatag cctttcattg tcaacatgaa acgtaacgtg ttcagagtga
     8341 tataagcaaa attaatgagg aagatcatcc ccaacatttt tggatcaatc atttgtaaaa
     8401 ctcctttcat ttatataaca acaatatcaa aaaaaatcgt aagtgaagaa aaattgaaac
     8461 tttatacata caaaaaaatg ataaaatgat tataatagaa ttataaagat aggttaggtg
     8521 attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc acgtgctaag tctccttctc
     8581 agtatatact taggtgtcta ttcgattcaa cagccaattg aggtagagaa cattgttttt
     8641 cagatgtcgc tgacagatgg tttaatttta agcgtttttt tctttttgat actgtttatt
     8701 ggtaatattt tcggtagcgt tgccagtttt ttgaattttt ctatttatcc actcctttcg
     8761 ctagcattag gagtggtggg actttgttct ctgtgcttat ttcccgagcc attcagtcct
     8821 gcatttatat tatttggtac actgaacctg ttccagacaa ctgttggagc atggttgcta
     8881 tggcgatcag gaaatttaat gaaaataggt gagtaacaat tgaatccaat tatattgaaa
     8941 cgttctgtcc atcatattcc agtgttagaa gttgttctga aagaattaag agaggaacgt
     9001 attcccgttg tcatctatta tcacggatgg cagacaagta aggagttagt tttgacgcaa
     9061 ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc cagatgcgat gaatcatggg
     9121 gagcgcaaac agccggtttc ttctattcca tcctatacat tttgggatag tatctacgga
     9181 aatctgtttg aatttgacac acttatcaag catttagaaa aaagaggtct tctacatgat
     9241 cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa catgcgcctt gttggctaaa
     9301 catcctcata tagtagcggg cgtgtgtttg atgggttccc ctgcaccttt ggaatatgga
     9361 aaagaaatcg taaggcgcgc atcggaatat aattatgtcg taccaaaaga ctattttgat
     9421 ttgatttcat gggttaagag ctatgatttg tcattgcagc cggaaaagtt agctggacgt
     9481 cctctgttct tttggcatgg acaagctgac gaaaaaatcc cttatataca aacggaacga
     9541 tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa ttgtttttcg ttcctcaaaa
     9601 acacagcgac atatggtaca aataccacta atggaagaag cttccacttt tttagcaaaa
     9661 aatttgataa aaaagtataa ataattgaaa cggaaaatca ctcatgacga tagaggattt
     9721 tccgtttttt attttgggct ataataaact ctgttaggaa aatttggtat aattgatagt
     9781 aagaatgaca acatgttaga aaggaagaga gcatgcccac tttagcagat gttgcaaaac
     9841 gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa tcatccggaa caggtgactg
     9901 acgaattgaa agagttagtt ttctcagcaa tggctgaact ggattaccgt ccaaatattg
     9961 ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa attatttata ctggaagaaa
    10021 tcgacacaac tgagccctat tacatgaatt tgctgatggg aatcgctaaa cggatcggta
    10081 aagcgcatta ttctctccaa ttagttacaa atgatgcctt cgacgtcggt tcttgtgatg
    10141 gatacatcat cacaggtgtc agaaaaaaag acttcgattg gatcgaccgt ttggaaaaac
    10201 cagttgtctt gtttggggaa aattctgaag gatatgatta tgtagacagc gataatgaat
    10261 atggtacagc aaaagcaacg caatatgcct tagaaagaga ttatcaaacg attatttata
    10321 tcggtatcga agtggacgag ccttttgaaa tttcaaggga aactggttat ttatctgtga
    10381 tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc aaatcattcg acagaagtag
    10441 aaagatatat gacagaaaac tgggcagatt ttacagataa aacttgtttt gtatgtagtt
    10501 cagatcggct agcactagga gcagtaagag ggattataaa tgctggagga cacttgcccg
    10561 aagaatttgg tgtgattgga tttgatgggg tatttttaga ccaaatcagt tcacctaaat
    10621 tgacaacgat caagcaagat attatacgta tgggtgaagt atgtggagaa atgttgttaa
    10681 aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg tttttttcct gaactagtta
    10741 tcagagagac aacgaattaa gtcttattac tatatatata tttatgaaat tcatcgctat
    10801 tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta ttattgagaa
    10861 aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa catttccgtt
    10921 ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag cgagtattat
    10981 ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa cagatgaaag
    11041 aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg
    11101 gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa ttaccggatt
    11161 ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt tttggtgatt
    11221 cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact acacgaaacg
    11281 catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat gtacccgcat
    11341 cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc gaatcgatga
    11401 aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat caagaaaaaa
    11461 gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag gaaacaaagc
    11521 agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca ttacaaggat
    11581 tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa gtgatataca
    11641 aatttatatt cggctacagt tggaaaggaa acgctgcggc gtttccttat tttatactct
    11701 atccgaaagt ttatatcgta tcctcaaatt tttttgagtt taagcgaatt ttttctccca
    11761 ataagtaaga aaataatggc aaaataaaaa aacaaactat tttcgcagat cgtttgcaaa
    11821 aatagtttat ttttttataa agaagatttt actgaataat atcattttag tttacataat
    11881 gatatttctg taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa
    11941 gaggatcaag tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag
    12001 atccaatttc cagatacttc actttattca atgtataaga tagaatagaa cagctaagta
    12061 atgtatacag aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag
    12121 taatcaagaa aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa
    12181 tgatatctgt tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag
    12241 aaagaatacc tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa
    12301 ggctctccca taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa
    12361 aagcgtgttt tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg
    12421 aagtgacaga aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc
    12481 caccatctgc cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg
    12541 cgcctaaaat gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct
    12601 tttcttttga aaattttcag caacttcttt attatactga cttatactct tacttcaaga
    12661 ggaaaatgaa aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa
    12721 aaatgcgagc agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc
    12781 aaggagtttt tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata
    12841 aagcaaatca atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca
    12901 tctttctttt tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac
    12961 tcattcctct ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa
    13021 gaaaacaaga cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt
    13081 catgatacag tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt
    13141 ctactttatt ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag
    13201 ctgattccaa gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa
    13261 ccaaaaagtt gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta
    13321 tttttttctt ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa
    13381 ggaaagaaat gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata
    13441 caaacattaa gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt
    13501 tctctcattt ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc
    13561 aatcataaaa aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct
    13621 tcacttgcct tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg
    13681 attgcacttg ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct
    13741 gaaattgacc cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc
    13801 ttgctgaaag tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc
    13861 ttagttttaa tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg
    13921 acatttattt tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc
    13981 agttctgcct tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa
    14041 cctcgatatt ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta
    14101 tatcagtctg ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa
    14161 cctgaaatcc tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt
    14221 gaattaaaac cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa
    14281 atcgatatct atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa
    14341 gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg
    14401 aataagcaat tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg
    14461 gcagtcaaga gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct
    14521 atctctgatc agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat
    14581 aaaggaatcc aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt
    14641 ttgcgttacc aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat
    14701 agccagctca aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct
    14761 tatcaaggcg caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa
    14821 gcactgaata aattaagcgg aaaaataaca gaggatcaaa tgattgatat gaattaccaa
    14881 gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga
    14941 ttagtaaagg aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt
    15001 atgatggaaa gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg
    15061 ttttggttgg tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat
    15121 ctaaaccatc agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc
    15181 agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga
    15241 cagtcaagca aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag
    15301 cagaccgtgt agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc
    15361 gtatgcctag agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg
    15421 ctgcaagccc agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa
    15481 gaaactcatt acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt
    15541 tcgttacgca caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg
    15601 atggagaaat tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact
    15661 acgttcggca tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag
    15721 tcgtcaatgg atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg
    15781 atacgcctat gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag
    15841 aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag
    15901 aggaggaagg aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta
    15961 agtgctgctt atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta
    16021 tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta
    16081 gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc
    16141 aactgggaag aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga
    16201 aaaaaacaat tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat
    16261 gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc
    16321 gggcaaagac ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac
    16381 tttttatcat tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct
    16441 tttgagctag ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat
    16501 caacggccat taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa
    16561 gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact
    16621 cgctatcgtt tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact
    16681 gggagacagc cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa
    16741 cacggaattg cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga
    16801 gatattgttt caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat
    16861 gtagctaagc ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg
    16921 atcgtctacg ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg
    16981 tcagatcaag tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa
    17041 ccagttgcta aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg
    17101 attagcgagc aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg
    17161 actaaaaaag agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc
    17221 tatttattga aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct
    17281 acactttaaa aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg
    17341 tgaacttaga cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg
    17401 cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag
    17461 aaaggaggta acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta
    17521 attatttggc agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct
    17581 gctgctgtat tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg
    17641 acattaaatg aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc
    17701 ttggcaatgg attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca
    17761 caaatgttgc caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg
    17821 cttccaaaag tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg
    17881 gatacattga ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat
    17941 acagggaaga tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt
    18001 ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct
    18061 caagcaggct tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt
    18121 tttgctgcga tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta
    18181 ttgaaaaaag ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa
    18241 aattatggct tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa
    18301 caagtagcaa acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac
    18361 cgaatactaa tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg
    18421 gtttaaatgt ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg
    18481 cggacaaagc acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg
    18541 gacagaatat accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg
    18601 ccaccctatc agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag
    18661 gttggcaaag tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg
    18721 acttttcaaa actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat
    18781 cctcagatat ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta
    18841 ttgaactaaa ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg
    18901 taattattac gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg
    18961 atgccacgaa aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt
    19021 tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt
    19081 caaaaaatta tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca
    19141 attatacagc ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac
    19201 tattcactaa tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt
    19261 gaatgtagtc attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga
    19321 tacctttaca tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt
    19381 aacaggagtc cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg
    19441 tctccataca gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt
    19501 ctatcaaaac gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt
    19561 aagggattat ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt
    19621 atctggcggg atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta
    19681 cttgcttctt gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga
    19741 ctggatgttg aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat
    19801 cgaagaagca atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt
    19861 gattgctgag attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc
    19921 agagttgaag caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc
    19981 attgtcattt agatgggagt cagagtttag cactccttca gcaggaacac acgattttga
    20041 cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta
    20101 agactcaagc cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac
    20161 aagttgagcc ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt
    20221 ggacgaatgt accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag
    20281 ccataaatga gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt
    20341 atatccaagc ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac
    20401 aagactatat tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag
    20461 cagtctggca actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac
    20521 tacatgcagc agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc
    20581 ttgaagaaca gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa
    20641 aaaatactta tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga
    20701 cacaactagt gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa
    20761 taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac
    20821 cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac
    20881 catttaagta agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt
    20941 aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag
    21001 tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt
    21061 tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa
    21121 cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt
//
LOCUS       DASJPH010000049        20975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJPH010000049 DASJPH010000000
VERSION     DASJPH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.049"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..639
                     /locus_tag="U3995_001627"
     CDS             <1..639
                     /locus_tag="U3995_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796954.1"
                     /translation="TQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYD
                     VQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQ
                     VTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPM
                     LTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1342..1557)
                     /locus_tag="U3995_001628"
     CDS             complement(1342..1557)
                     /locus_tag="U3995_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN1796955.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1576..1812)
                     /locus_tag="U3995_001629"
     CDS             complement(1576..1812)
                     /locus_tag="U3995_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN1796956.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2070..4223
                     /locus_tag="U3995_001630"
     CDS             2070..4223
                     /locus_tag="U3995_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN1796957.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4192..5607
                     /locus_tag="U3995_001631"
     CDS             4192..5607
                     /locus_tag="U3995_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN1796958.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5640..6674
                     /gene="sppA"
                     /locus_tag="U3995_001632"
     CDS             5640..6674
                     /gene="sppA"
                     /locus_tag="U3995_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1796959.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6676..7197
                     /locus_tag="U3995_001633"
     CDS             6676..7197
                     /locus_tag="U3995_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1796960.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7194..7580
                     /locus_tag="U3995_001634"
     CDS             7194..7580
                     /locus_tag="U3995_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1796961.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8076..8148)
                     /locus_tag="U3995_001635"
     tRNA            complement(8076..8148)
                     /locus_tag="U3995_001635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8113..8115),aa:Lys,seq:ctt)
     gene            8529..9098
                     /locus_tag="U3995_001636"
     CDS             8529..9098
                     /locus_tag="U3995_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1796962.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9085..9945
                     /locus_tag="U3995_001637"
     CDS             9085..9945
                     /locus_tag="U3995_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1796963.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10135..10839
                     /locus_tag="U3995_001638"
     CDS             10135..10839
                     /locus_tag="U3995_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1796964.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10844..12679
                     /gene="walK"
                     /locus_tag="U3995_001639"
     CDS             10844..12679
                     /gene="walK"
                     /locus_tag="U3995_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1796965.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12676..13992
                     /locus_tag="U3995_001640"
     CDS             12676..13992
                     /locus_tag="U3995_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796966.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            13993..14862
                     /locus_tag="U3995_001641"
     CDS             13993..14862
                     /locus_tag="U3995_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1796967.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            14917..15726
                     /locus_tag="U3995_001642"
     CDS             14917..15726
                     /locus_tag="U3995_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1796968.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15829..16458)
                     /locus_tag="U3995_001643"
     CDS             complement(15829..16458)
                     /locus_tag="U3995_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1796969.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16628..17920
                     /locus_tag="U3995_001644"
     CDS             16628..17920
                     /locus_tag="U3995_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1796970.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(17996..18658)
                     /locus_tag="U3995_001645"
     CDS             complement(17996..18658)
                     /locus_tag="U3995_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1796971.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18883..19167
                     /gene="groES"
                     /locus_tag="U3995_001646"
     CDS             18883..19167
                     /gene="groES"
                     /locus_tag="U3995_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1796972.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19218..20843
                     /gene="groL"
                     /locus_tag="U3995_001647"
     CDS             19218..20843
                     /gene="groL"
                     /locus_tag="U3995_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1796973.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7152 a         3407 c         4236 g         6180 t
ORIGIN      
        1 acacaaagtc cgttagaagt taaaaaatac ctcaaacaat tccgaccaaa gcaaggaata
       61 tatttcttag atattgattt aaatcatgaa gttaacggta tagaattagc agaagtaatc
      121 agaaaatatg atgttcaagc aaaaatcatt tttacaacta ctcatgatga gatgttaccc
      181 gtaacaataa aaagaagagt tgaaacgtta ggatttgtaa caaaagatca aacactagat
      241 gagtatcgaa acgagattgt tgagttattg ttattagcgc aagaaaggat agatgcaaca
      301 aaacaggtta cgaatcaagc atttgttttt tccataggtt ctcaaacatt tacttttgat
      361 ataaatgaca tttattttgt agaagcttca aaattaccac atagattatc attgtgtact
      421 aaagatgggc agtacgaatt ttatggaaga attagtgagc ttgaaaagaa atatcctatg
      481 ttaacaagaa ttagtcgagc gtgtttagta aatatcttta acgttaaaga aatagatttt
      541 aaaaaacgtt cactatattt tgattcagag ttggccagaa actttacttt agggaaagca
      601 caaaaaatta aggagaagtt gaaagaaagc actgtttagt ggttttttct ttagggagat
      661 tgcatttttc taataaaata gattccttca ctagtgcaaa tttttctgaa tcctattaaa
      721 gttgtaccaa tttttatagc tgttattagt tacgtgcata acagcaatat tagttactgg
      781 aggaatattt ctatggtctg gaaccaaaaa agggccaaat gaatagttct tttcttgttt
      841 ttattaagta ccataaaggg gaattaaaat taaatgtatg gtggaagatg cgaaagattt
      901 ggcaaattct actaattttt agaaagtgtt tttcattttt tagttaaaaa atttgtcctg
      961 tcaagtgcaa tcctatgatt gtacttgata ggatttgaga taattacttt cctgttttta
     1021 taaatttaac ttgcaggaac aggctttaat gcacatccga caagtacatt accaatacct
     1081 cctgtaacgg ccgaaatagg tcctcctgca gcaccaacta cagcgctgca agtccttttg
     1141 taaaaatatc tctaccatta catttccaaa gaatttttat ctgactttaa taaaagataa
     1201 aaatttaata taattataaa aactagcaca agaagcttta gtaaaaagtt ctgtccattc
     1261 caaacttttg aaaagataaa gaacatacct atgttcgtaa tcgacacaaa ccaatcttga
     1321 ggtttgatat tttttaattg tttacgcacc ggtccacatg ctgaatccag cacctaataa
     1381 agctccacct acaccacaag cagctattgc ataaggtcct ccggctgaac aaagttttac
     1441 tcctgctgtt gccccagaaa aagtagtgcc taaaactccc acggcactcc caccttgaat
     1501 atttttcaac tcttctgaag ataatataaa tgttgttttt gtgtttttaa tatgcatact
     1561 acttattctc cttttttatt tttttcgagc atcccagcca gctgcagcac ctccagcgac
     1621 agctccatat aaccctccaa caacaccgca agctggtccg caaattgctg ttcctaaacc
     1681 tgcaccacct actgcccctg tggtaagtcc acctacaact tgccccaatt ttccccaagc
     1741 attattgcct ccaccaacag tttgtgcatt tccacaatac ttagttcctt tacattttgc
     1801 atgtttaaac acgcttcttt aaataatatt ttttacggtg ctactgtaac atacgtattt
     1861 gattaacagt aaaaatcttc ctgaaactta tcaagtttat tcttgaatag taaaatttcc
     1921 aaaataaatt tgattcagga atgaaaaagt tagtgttcaa gaactgtttt caaagttttt
     1981 gtggaattat gagacaatat tttcactgta ttaaagctat attgagaatt tttctataaa
     2041 aattttttag aaggagttgt gttagataag tgataaaatc atttcctttt gtgcagcaac
     2101 aagatgaaaa agattgtggt gttgcttgcc tttcgatgat tttaaaatat tttaaaactg
     2161 atatcccgat tcataaatta cgggacctat ctgggactga tttagagggt acttcagcat
     2221 ttggattgaa aaagacattt gaaaaattgt cttttgattg tatggctatt caagcagata
     2281 gtgagatctg gaaagaaaga gatcttatct taccattaat tgcacatata ttggttgata
     2341 acagctatat gcactacgtt gtaatttata aaacgaaggg aaacgtacta tggattgcag
     2401 atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt tcaaaaagaa tggacaggta
     2461 ttcttttatt acctatgcct agagaaactt atattcctat taaagaaaaa gtagctggat
     2521 taagttcttt tttgcctata atttggaaac aaaaaggatt agtattccac atcgttttag
     2581 catcgatttt cattacgttt tttggcatag gaagctcata ttattttcaa ggtattcttg
     2641 attttttcat ccctaatcaa gcacgttcaa ctttaaatat aatttccatt ggcttgatcg
     2701 ctgtctattt atttcgggta ttgtttgaat atagtcgtag ttatcttctt attattttag
     2761 gacagcgaat gagtatggca attatgcttc agtatttcaa acatgtgtta actttaccaa
     2821 tgaatttttt tgctacgaga aaatcagggg aaattatttc tcgttttctt gatgccagta
     2881 agattattga tgctttagca agtgctacgt tatcaatatt tttagatatt ggtatggtgt
     2941 tgcttgttgg ggttacgtta gctattcaga atagtacgtt gtttttaatc actctatgct
     3001 ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa aagctatgaa aaatcgaaca
     3061 gggaagaaat ggcagccggt gcagctgtaa attctagtat catagaaagt cttaaaggaa
     3121 tagaaacaat taaagcatat agcggtgaag agaaagtata taaccgtata gaccatgaat
     3181 ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct tgataatatt caacaaggaa
     3241 ttaaacattt gattcaatta attagcagtg cattaatttt atggctaggt tcttattttg
     3301 ttatggataa tagaattagt ctaggtcaac ttatcacata caatgcttta ctagtatttt
     3361 tgacagatcc tttacaaaac attattaatt tacaagtgaa aatgcaaacg gctcaagttg
     3421 caaataatcg tttaaatgaa attttcgcga ttgattcaga aaaatcgaac cagtcttttg
     3481 aatatatgct ttctgaacaa ttatttcaac aagatatcac tgtaaaaaac gtttcattct
     3541 cgtatagcat gaaggcccca acgttgaacg aaatttcatg tacaattcct tcgcacagta
     3601 aaacggctat agttggagtt agtggttctg gaaaatcaac tcttgcaaag ttatttgttc
     3661 gtttttatga agcatcagaa ggatcgatcc attatggaaa cattaatcac ttagacattc
     3721 cttttcagat attacgcgat cgagtgacat atttgcctca agagtcattt ttctttagtg
     3781 gaacaatcat tgagaatctt atattcggtt tagatagccc accaagtttc gaacaaataa
     3841 tagaagtttg tgagatagtc caactaaatg aatttattaa tcaacagcca ttacgttacg
     3901 agacattgtt ggaagaaggt ggaaataatc tttcaggagg gcaacgacaa cgattagcct
     3961 tagcacgggc tttattaaaa aatccggaca tattaatttt agatgaagca actagtggat
     4021 tagatacctt gttagaacat gcaattatga attatctcct caatttaaaa gaaaaaacga
     4081 ttatttttat tgcgcatcat ttaccaatag caaaagcatg tgatcaaatt ttagttctcc
     4141 atgaagggca tttggtagaa aaaggaacgc acgatgaact tcgttttgcc gatggtatct
     4201 atcaaaaatt atgggaaatc taggagaggg aaacaattta tgcagaataa taattggttc
     4261 gaacattcta gtatctatac taaacaacat catagcttct accgttgggt gttataccct
     4321 gtaattcttt ttttatgtat tattggattg tttttaacct ttgctaaaaa agaggttgtt
     4381 attcggacaa cggctcaatt gaaccccgaa aaaatagaga agttacaagt gccattagaa
     4441 gctaaaataa tggaaaactt cctaaaagaa aatcaatttg ttcataagga cgatgtgttg
     4501 gttagattgg attgttcttt aatagagaat gaaaaggctc agatagaaca agaaaaccaa
     4561 cgtattactc aacaaataaa gatggctcag ctatttattg aaagtataag taaaggaaaa
     4621 aatttgtttt caacggatga cagttttggc tacagtaatc aattaaagag catgttgtca
     4681 gaaaaagaat cactccgcta cgctttgaag caaagtgaat taaatgatca aaagcaatta
     4741 gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa ttgagagttc agatagtaaa
     4801 ttacaagaat ggcaacaagt acaggtagct tggagtaata atcaatcatt aaaagatttt
     4861 tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac aactaaataa tgtttctgat
     4921 gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca taaatgaaca aattgagcaa
     4981 ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta aattagttaa accaactact
     5041 tctgagaatg acagaattag tcaaacggaa aaaggaaagc aagagctaga acaagcaata
     5101 acaactgtaa aacaaacagt tacagagcta aatgaaaaac aagagaagaa ccagtcagtt
     5161 atcaaaagtt tggacgatca actttcaaaa ggaatactaa aagctcctgt atctggaacg
     5221 gttcatttaa atgaagaaac aaagggacaa gcagaactag ccaaaggaac agtgcttgca
     5281 gaaatttatc cgatccacga aaaatcaaaa atgaaattta cagcactttt acctgcgaat
     5341 gaaagtacct atattaaaga agggatgaag gttcatttta aattggatca aaaaggtggg
     5401 gctccgatta cgatcgacgg aactttagat gaaatttcag aaaattctac tgccactgag
     5461 aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa ctcaatcgtt tccgtttcgt
     5521 tatggattaa ctggagagtt gtctttaatt gtaggagaaa aaacatattg gcaacaaatt
     5581 gtcgatgtgt ttctaaataa aaaataatta tttttactaa gaatatagga ggagtttata
     5641 tgaataaaag aagatggatt gctgtgggag tagcgtgcgg attattgtta ctatccatta
     5701 tcgtcgcgct aattccagga aaagataaag aggaagcatc aaatacaacc ctaacaggaa
     5761 taaataagat tttttacgga tctaacgaaa tcacagaaga aacgttagaa gaaggcgcat
     5821 caaataaaaa gattgtcaaa ctatccgtca atggagttat tgcagataca ggtgaaagca
     5881 atctttttag tagggaacaa tataaccatc agaatttttt aacacagcta aagaaaattc
     5941 aagaagacaa agcggttaag ggagtgttat tagaagtaaa ttcacctggg ggaggaatat
     6001 atgaaagtgc tgagattgct aaagaaatgg caaaaattaa aaagctagat attcctatct
     6061 atactgcatt taaaaataca gcggcaagtg gagggtacta catttctgct ggttcagata
     6121 aaatatttgc tacggaagaa acaacaactg gatcaattgg tgtcattatt tctggtttaa
     6181 attattctgg cttattagaa aaacttggcg taactgatgc gacgtataaa agtggtgcat
     6241 taaaagatat gatgtcacct caacacaaac cttcagagga agagaataaa gttatacagg
     6301 aatttgtgat gagtgcttat gatcgttttg tcaatgttgt agcaaaagga agaaatatgg
     6361 atactaatgc agtgaaagaa cttgcggatg gaagaattta tgatggtaac caagcagtag
     6421 aaaatgggtt ggtggatcaa attggttact ctgaagatgc attagattcc ctaaaaaaag
     6481 agaagaaatt aactgatgcg acggttatag aatataaaaa tgacacaact ggctttgctt
     6541 cttcttggtt aggtaataag attgctgaat ggcaagggct aaaggcaact acatcagatc
     6601 gtgtaatttc agtttttgaa aaacttggat tttctgatac tccaaaacca atgtattact
     6661 atggagggct ataaaatgaa taagaagagt ggcattgaaa ctttgcctaa ttatgtattt
     6721 gcgggatttt ggttacgatt ttttgctttt cttattgatt taatttgtat tagcatcatc
     6781 acccatgcta ctataggtct cttttacaaa atcatgggac tggaatctga caaatccatt
     6841 attacttttt atggtatagc gtcattagct atttatttaa cctattttgt acttttgact
     6901 aagctgaatt caggacaaac aattgggaaa atgatttttg ggataagagt agtgagcttt
     6961 gaggaaaaag agctaagttg gcaaactatc tttattcgtg agtgtatatg taggtttata
     7021 ttaaaaaatt ttgtatttca aattgggtat ttggtggctg ctttcacgcc caagaaacaa
     7081 catattgggg attatttttc taacacgtca gtggttgtta ttcgttcatt agaagctatt
     7141 aactatggtc aaaaaatttc taattctaaa acacttaatc tggagagaat taaatgaata
     7201 agagaaaaac agagtttatt ttgattttaa ttagtggaat aacagggatt ttatgtagtc
     7261 ttttagcatt gatgggtggt atgtttctat cttctattga ttcaactact ttaaatacta
     7321 cagaactaac cagtaaggat atagatatgt tagtcaatct aagcgggaat gttgttttat
     7381 ttggaggaat taccctagtt ttttcgataa tgattttcat aataggtttt ttagtaagaa
     7441 aaaatcaaaa aacagtggtc tatggtatta gtttactcat tttatctatt attccatttt
     7501 ttcttttaac attattgtgg gtaattcctg gagttttagg tattattgcg ggattgatgc
     7561 ttctaataag aaaatcttaa ttaggcaatg tgtagacaac gtgaaacgat taatcaatta
     7621 aaagctaaat acgttttgag aaatgataac ttgtagtcga tggaagacat gtagataaaa
     7681 aaactacgtg tcttttattt attctaattt tttcgaaaaa aagacactct ctgattgttg
     7741 tattaatgga aaaggggaaa gcagcaagta aatttagttt ttctattaaa tattcttcac
     7801 tttttaacct tttatagtac ttagatttaa tcctatatgt ttagagagtt cattggcagc
     7861 atgattttgc aaagcttctc gaattattgt tatattagaa actttactgt tcataattat
     7921 gcttcttttt taactcaaag tccaaagggc tacagtggac tttgagttaa aaataaaaag
     7981 ccctagttat tttgaaaaat aaaaaaagaa cagcgtaaat gttttcgaaa tgctttcgca
     8041 ccaacattta cctgttccgt gggtttggca cccaatgggc cgtgaggggc tcgaacccgc
     8101 gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt acaaaaagta
     8161 cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag aaaatcagaa
     8221 aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct cgctattata
     8281 taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt ttttcatttt
     8341 ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa aagacaagaa
     8401 ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca cctagttaaa
     8461 gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg aaaaaaggag
     8521 cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa ctagaaacat
     8581 tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac caagtcagaa
     8641 cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca ttgcttacct
     8701 cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt aaaaaaagat
     8761 ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa gtattgacta
     8821 ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt cgtgaagcct
     8881 ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa ttagcacaag
     8941 gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt gctgtaagag
     9001 cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg
     9061 aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt agtagattca
     9121 ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat cccattgaaa
     9181 attatttata aagatcaggt atatacagat aaaattgaca ttacccctga agagatctat
     9241 caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc gatcacagaa
     9301 atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac aatttccagt
     9361 ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga agaattggaa
     9421 gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc gatccaagca
     9481 gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct gaatgagtta
     9541 gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca aaaaggtgga
     9601 cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc aatcatctca
     9661 tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg
     9721 gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt caacttagct
     9781 gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact taaaagagag
     9841 ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt agtccacact
     9901 ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa
     9961 cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa aataataaag
    10021 tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg aaacaaaagc
    10081 agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct ctttatgaaa
    10141 aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt caatttagcc
    10201 aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga aaaagttgct
    10261 gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga cggcttagaa
    10321 gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac agccaaagat
    10381 tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt gaccaagcca
    10441 ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct
    10501 gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt gacgatccat
    10561 ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca tcgtgaattc
    10621 gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga acatttatta
    10681 caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt aactgtacgt
    10741 cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt gacacgtcgt
    10801 ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa gaaaagtccg
    10861 cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt tattgatctc
    10921 gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga tcaatacatt
    10981 tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc cggaattgaa
    11041 tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa
    11101 cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg
    11161 aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa
    11221 tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg tgaatatcaa
    11281 tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt acgtcaaaag
    11341 taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct ttactgcctc
    11401 actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt cgattacaca
    11461 gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact acagtcggaa
    11521 agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca atcagttagc
    11581 tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt
    11641 gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag tcattacgat
    11701 caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg gacaatcgat
    11761 tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag aagatccaaa
    11821 cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga tcttgcgagt
    11881 agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag ctgtcttgca
    11941 tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc
    12001 ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat tgagcgaagg
    12061 tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag atgaaacaga
    12121 ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata caggtaatac
    12181 ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc ttgaccggtt
    12241 tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag
    12301 agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata atatcatgaa
    12361 taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat tagaaacgca
    12421 taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa aagatctgaa
    12481 ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac aaggtgggac
    12541 aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg ctatttgggc
    12601 agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg aaccttatga
    12661 ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact cattttgatg
    12721 gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg aaaaagttca
    12781 atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc
    12841 gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca agaaaccaac
    12901 agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt tggaaagctt
    12961 tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct gaactctggt
    13021 tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc atttgattta
    13081 gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac tagaatacaa
    13141 ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct cgtatatgaa
    13201 gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa agacacaaaa
    13261 tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac gaaacattcg
    13321 atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact ttttagaaat
    13381 gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga attgattttt
    13441 tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga ttttcgtgga
    13501 gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc ctatattagc
    13561 aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa tacgatcgat
    13621 tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg acagattggt
    13681 gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac cagtttgaat
    13741 acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc
    13801 atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt gatcgttggc
    13861 tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc tgaatggtat
    13921 gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt acctggattg
    13981 gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg
    14041 ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa caatgtcatt
    14101 cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat cacttacacg
    14161 gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca gaccaatctt
    14221 agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg
    14281 tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga aaagaacgtg
    14341 atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa agacaatatt
    14401 ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga agaaccacta
    14461 gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac agcaaaaatc
    14521 gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat gcagacgcat
    14581 ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga ggaagcaatc
    14641 aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg gcgtcagcta
    14701 gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta
    14761 gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt cagcaatacg
    14821 atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga gtataatgaa
    14881 aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa accagctttt
    14941 aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga aagtgaaaaa
    15001 aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa
    15061 attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca tcgagatcat
    15121 atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc caatcagcag
    15181 acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa acatctattt
    15241 gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg cgtctcccat
    15301 gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg
    15361 acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc cgatggttat
    15421 ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc gtggaattta
    15481 aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc acttgtcatg
    15541 actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag caaggaaaac
    15601 aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa agattttggc
    15661 gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga gctattttcg
    15721 atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa tcagggttgg
    15781 ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt atcgactgac
    15841 tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta atgcaatcgc
    15901 gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat aatcatcact
    15961 taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg gactgatata
    16021 agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca tattgaaagc
    16081 tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg tcttttctgt
    16141 atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg ttccttcaag
    16201 catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt tgggagcgtt
    16261 gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact caaataaatc
    16321 cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga ttgccgtcac
    16381 gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc cagtagctcc
    16441 aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt aagcagttgc
    16501 tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct ttcatggttc
    16561 gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa gttgaaggag
    16621 ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat ctgttgtgaa
    16681 tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa taattattgt
    16741 tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc agtggataga
    16801 caaaacagga ttttctatgt attttacaca agtataccag gctacatttg atgtgatagc
    16861 cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt
    16921 accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac taaatactca
    16981 gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa ctgggctcat
    17041 catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc aaagagagaa
    17101 gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg ttatagtcac
    17161 ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg ttgttcaagg
    17221 agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt ttggtgtgct
    17281 tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat ctgcgatggt
    17341 caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag aaatcttagc
    17401 ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc aatttctcga
    17461 tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt ttatgacggt
    17521 ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac cagccttttt
    17581 caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga tgatggctgt
    17641 tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt tatattttgg
    17701 attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga tcctctcggg
    17761 tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt caattagttt
    17821 tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa aggaaagaca
    17881 gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc
    17941 gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt catgtttata
    18001 ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg
    18061 tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag aagaaaccta
    18121 ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc aaagagctga
    18181 tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata ctgcggcgga
    18241 atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt
    18301 ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg ccaaacaata
    18361 cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt ccaaccaatc
    18421 cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc gctaagggtt
    18481 cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac atgactgcag
    18541 ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg atcaagggtg
    18601 agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt aaagacatgt
    18661 aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat tgatttcaat
    18721 tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg atttaaaagc
    18781 ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc gaaggttaag
    18841 agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa accattaggt
    18901 gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta
    18961 gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg tgaaggccgt
    19021 ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca agttatgttc
    19081 gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat cgtcgcagga
    19141 aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc aaaaataatc
    19201 aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta
    19261 tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga cctaaaggcc
    19321 gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat ggtgtgacga
    19381 tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag
    19441 aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca actgttctga
    19501 cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat cctctaggca
    19561 ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat aatatctcaa
    19621 ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg ggttctgata
    19681 aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa
    19741 tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg cagtttgatc
    19801 gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct gttctagaaa
    19861 acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt ctgccattgt
    19921 tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat gtagatggtg
    19981 aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa
    20041 aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct attttgacag
    20101 gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca atcgaaaact
    20161 taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta gaaggttctg
    20221 gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa
    20281 cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca ggtggtgtag
    20341 ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa ttacgcattg
    20401 aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca ggtggtggta
    20461 cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga gacgtggcaa
    20521 caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg
    20581 ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta ggcactggtt
    20641 tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta gacccaacta
    20701 aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta ttaacgacag
    20761 aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca atggatccat
    20821 caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact gatcattagt
    20881 gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat aagaagaaat
    20941 tcacggaaat tgcttttcaa atttttgtga atttc
//
LOCUS       DASJPH010000050        20360 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJPH010000050 DASJPH010000000
VERSION     DASJPH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.050"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            291..1430
                     /locus_tag="U3995_001648"
     CDS             291..1430
                     /locus_tag="U3995_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1796974.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="U3995_001649"
     CDS             complement(1497..2072)
                     /locus_tag="U3995_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1796975.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="U3995_001650"
     CDS             complement(2077..3051)
                     /locus_tag="U3995_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1796976.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="U3995_001651"
     CDS             3334..4302
                     /locus_tag="U3995_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1796977.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="U3995_001652"
     CDS             4415..4693
                     /locus_tag="U3995_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1796978.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="U3995_001653"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="U3995_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1796979.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="U3995_001654"
     CDS             5150..5905
                     /locus_tag="U3995_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1796980.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="U3995_001655"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="U3995_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN1796981.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="U3995_001656"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="U3995_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN1796982.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="U3995_001657"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="U3995_001657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1796983.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="U3995_001658"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="U3995_001658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1796984.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="U3995_001659"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="U3995_001659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1796985.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="U3995_001660"
     CDS             10642..11184
                     /locus_tag="U3995_001660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1796986.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="U3995_001661"
     CDS             11207..12763
                     /locus_tag="U3995_001661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1796987.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="U3995_001662"
     CDS             12777..13679
                     /locus_tag="U3995_001662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1796988.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="U3995_001663"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="U3995_001663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1796989.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="U3995_001664"
     CDS             15122..15544
                     /locus_tag="U3995_001664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1796990.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="U3995_001665"
     CDS             16013..16243
                     /locus_tag="U3995_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1796991.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="U3995_001666"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="U3995_001666"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1796992.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="U3995_001667"
     CDS             17654..17830
                     /locus_tag="U3995_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1796993.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="U3995_001668"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="U3995_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1796994.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="U3995_001669"
     CDS             18325..18708
                     /locus_tag="U3995_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1796995.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="U3995_001670"
     CDS             18877..19761
                     /locus_tag="U3995_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1796996.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="U3995_001671"
     CDS             19875..20111
                     /locus_tag="U3995_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1796997.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="U3995_001672"
     tRNA            20199..20279
                     /locus_tag="U3995_001672"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="U3995_001673"
     tRNA            20286..20357
                     /locus_tag="U3995_001673"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DASJPH010000051        19783 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJPH010000051 DASJPH010000000
VERSION     DASJPH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.051"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(522..1136)
                     /locus_tag="U3995_001674"
     CDS             complement(522..1136)
                     /locus_tag="U3995_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1796998.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1742..2119)
                     /locus_tag="U3995_001675"
     CDS             complement(1742..2119)
                     /locus_tag="U3995_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1796999.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2419..3300)
                     /gene="galU"
                     /locus_tag="U3995_001676"
     CDS             complement(2419..3300)
                     /gene="galU"
                     /locus_tag="U3995_001676"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1797000.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3343..4362)
                     /locus_tag="U3995_001677"
     CDS             complement(3343..4362)
                     /locus_tag="U3995_001677"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1797001.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4995..5999)
                     /locus_tag="U3995_001678"
     CDS             complement(4995..5999)
                     /locus_tag="U3995_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1797002.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(6012..7061)
                     /locus_tag="U3995_001679"
     CDS             complement(6012..7061)
                     /locus_tag="U3995_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1797003.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(7078..7899)
                     /locus_tag="U3995_001680"
     CDS             complement(7078..7899)
                     /locus_tag="U3995_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797004.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7886..8665)
                     /locus_tag="U3995_001681"
     CDS             complement(7886..8665)
                     /locus_tag="U3995_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797005.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8682..9152)
                     /locus_tag="U3995_001682"
     CDS             complement(8682..9152)
                     /locus_tag="U3995_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797006.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9164..9583)
                     /locus_tag="U3995_001683"
     CDS             complement(9164..9583)
                     /locus_tag="U3995_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1797007.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9664..12441)
                     /locus_tag="U3995_001684"
     CDS             complement(9664..12441)
                     /locus_tag="U3995_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797008.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12632..12856
                     /locus_tag="U3995_001685"
     CDS             12632..12856
                     /locus_tag="U3995_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797009.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13635..15350
                     /locus_tag="U3995_001686"
     CDS             13635..15350
                     /locus_tag="U3995_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797010.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            15353..17116
                     /locus_tag="U3995_001687"
     CDS             15353..17116
                     /locus_tag="U3995_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797011.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(17156..17692)
                     /locus_tag="U3995_001688"
     CDS             complement(17156..17692)
                     /locus_tag="U3995_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1797012.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(18015..18437)
                     /gene="mgsA"
                     /locus_tag="U3995_001689"
     CDS             complement(18015..18437)
                     /gene="mgsA"
                     /locus_tag="U3995_001689"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1797013.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            18646..19755
                     /gene="ugpC"
                     /locus_tag="U3995_001690"
     CDS             18646..19755
                     /gene="ugpC"
                     /locus_tag="U3995_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1797014.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5922 a         4032 c         3404 g         6425 t
ORIGIN      
        1 cattttacaa tcattgaaaa attgaatttc attgcaaaaa aaataataaa cacttataaa
       61 tatccattat ctatccttca atttttgtgt attttatttc tattagaaaa aggtatcctg
      121 ttggacaatt ttgttatatc aggtaaactt cattgcaata atgataacaa atgtactata
      181 attactttgt aaagagtttt ggggaagact ctttcatcaa cgtgtattaa gagacaagaa
      241 agaatccata tggattcttt cttgtctctt tttccactac tttttatttt tctgacacta
      301 catattctta taactcattt ctcataactg tgaaacaact tataaaaata acaaacttta
      361 aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac aaaaaaagta
      421 gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca tttttatgtg
      481 gaacattatt tttttaagga actttgtcaa catgtggata actattttcc acttgtgtcc
      541 tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga
      601 gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc atcatgtaaa
      661 ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc actgctatgt
      721 tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg atgaccattt
      781 tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga
      841 agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct
      901 agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac aagatccggg
      961 gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata tatctgcaat
     1021 ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat cagcggctct
     1081 tctatcctta atttctcttc caattggtga taatagtcaa aataacataa actcatttat
     1141 ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta aggaagtaca
     1201 taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag
     1261 gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg cagaccatgg
     1321 aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa ttaggtcgat
     1381 gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc
     1441 ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg tttctatctg
     1501 aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa aaacattggt
     1561 atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga tacaacaagc
     1621 attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata
     1681 aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat
     1741 attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag
     1801 ttgtatgtct accttttctg cctatccgag actttctttt catatatggg atataaatga
     1861 ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca
     1921 attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg
     1981 tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca
     2041 caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa
     2101 cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt
     2161 tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt
     2221 tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa
     2281 gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg
     2341 acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag
     2401 caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata
     2461 tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat
     2521 atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc
     2581 ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg
     2641 ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc
     2701 taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg
     2761 acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg
     2821 tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg
     2881 agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt
     2941 tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg
     3001 gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc
     3061 cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct
     3121 tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac
     3181 aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt
     3241 tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac
     3301 ttgctgttcc tcctaaacga cgattaacta ttgatcatct atttaacgaa gagaaaattc
     3361 attctccatt tttccgtcac gtaacatgat ttcttttgct gcttttttga tatcttctcc
     3421 attatacaaa acattataga ttgtttgagt aattggcatc tcaacaccta gctgttgagc
     3481 tagctctact gctgcttttg tcgttgccac accctcaacg accattccca tgttttctag
     3541 aacttcttcc agcttatgtc cctgtcctaa caagttaccg gcacgccaat tgcgagaatg
     3601 aacacttgta caagtaacga tcaagtcccc cacaccactc aaaccgataa atgtcagagg
     3661 attggcaccc atcgcgacac caagacggct gatttctgcc aatcctcgag tcatgattgc
     3721 tgcttttgca tcatctccga atcctaatcc atggatagcg ccggcaccaa ttgcaatgat
     3781 atttttcagt gctgcaccag tttctacgcc aatcacatca ttatttgtat aaatcctgaa
     3841 ataatcattc atgaacagtt tttgtacata ggctgctgct ttttgatcaa tacttgcagc
     3901 tgtaattgtt gtgatatcat gtactgccac ttcctcagca tggctaggac cagataatac
     3961 aacaattgcc tgtctttttt cttctggaat ttcttcagcc aaaacttctg agattcgttt
     4021 atgcgtatct tgttctagtc ctttacttgc atgtataatg attggcttag tattcatttt
     4081 accaataagt tcctgtgcga ctgaacgaat cgcttttgtt ggaacaacaa ataaaatagc
     4141 atctgcgtta tccactgctt ctgccaattc tttatatccg ataatcgatt ctgggatcgt
     4201 caaatcaggt aaaaaatgct gatttgtatg atacttattg atttcatcaa tctgttcagg
     4261 tacatttccc caaatacgga catcatgccc gttttctgcc aatgtttgtg ctaaagcagt
     4321 gccccaagat ccaggaccaa gaactgcgat tttttgtttc atggatattc ctccaaataa
     4381 ataattgata atttccatta tagtatacat ttcttaaagt tgggactaat ttccattgtt
     4441 tttaagtgtt tgtttacttc ttttatcgtt tttacttgaa ataatcaatg atatgttaca
     4501 atagtctaca ttttattttt acactcgtta tgctttgctt gttcctaaga ggaagcagtt
     4561 ttgacaatgt tactaccagc attactatta ggaggttagg aaacttctcc tagtgagtat
     4621 tgatttaaaa tacataagag acagattgaa catttctaaa tctgtcaggt cctgcaacgg
     4681 acagattgtc tctgttgacg gttgacatag gaacttttgt tttagtcaac attctggata
     4741 caaaacgcta tgtaaactgg cgcaactcgt tgtacttgga cttttactac ggatccatgc
     4801 aacaactaaa tagtactatt gcataacaaa aaatggttgg cacccacatg tcccattaaa
     4861 cagattataa gttgaactgt agataatgca aaaaatgtgt tttataacgt aattgatgcc
     4921 aatattttaa aaaaataaag ggagcagtta tcgctgctct ctttattttt tatattcttt
     4981 ttatccagtc tatcctaata atcaaactga cgatagtagc ggcgtatctc taatggatgg
     5041 cagttcaact gctccacatg agcatcgatg cgttgtgtca ctacatgcat caataagaaa
     5101 gacaaaacac gtccgcgata tttttcactg ataccttcta ctggatagtc ttttgtgtct
     5161 atcaatgtat aattgctgca aatttttggc aaaagtttag ccacacgttc agataacact
     5221 ctctgttcat cttcgcctaa aaataatgtg actggggttg tttcttctac gatctctaac
     5281 gtcccgtgaa gaaactcggc agcatggata gatttcgatg gaagccagct ttgttcttcc
     5341 cagtaacaca tggcatatga ataaacagct ccccattgat tccctgctcc aataaaataa
     5401 tgcatactat cacgtcgatg tttttctgca aatgataagc caaatgcatc tgcttttttc
     5461 tctgcttcca ctaaccctgt tggtaaataa cgttcgagct gttcatagta ttctgaataa
     5521 gcttcgaatt ctccattcaa atacatcaat cgatctgctg ccataaaaaa tttgatttgt
     5581 tctgttccag gagctggata agtgaccaca aaatcgcata gctgagacaa cttactgttc
     5641 gctttatcga taaatccaat cagtgtcgct cctactttct tgatttgttc tactgctttc
     5701 actacttctt gagttgtacc ggtaacagag gacaaaataa cgatagaatc ttttgttaat
     5761 cttttgtttc cagtagtgta atattctgca gcatgctgta caaagacagg caagttgctt
     5821 ttcccattca tataagtgac agcttgcatc gaggaagcat acgttccacc gattcctaga
     5881 taatagacag cgtcaaattt ttccgcataa agtttatcga tgatttcttc tacttgcgga
     5941 cgtaaagcta gtgcgccttt tattgctttg atttgttctt cttcattgaa tttcagcatg
     6001 atttccacct cttataattt acttttcaag actgtatcaa agtcttcaaa aatctttttg
     6061 ttcaagtcaa tacctttagc tactgcggtt ttataagcga atagttgaaa cggaacgaca
     6121 agcgctaata cagacagata ttcatcacat tctattgcta atccaagtgt tttctcgttt
     6181 cttgcttttt ttgtggtaat ggtgagagtc tctccaacgt accgactcat atatctttgc
     6241 aacttttgcg aacgttcctt atttacactt tccccttcaa taaagaatag caaatgcgaa
     6301 gcatctgcct ccaggtatgg gccgtgcata taagcttcta actcaaatcc ttgtgacgga
     6361 acgcggattg tttcagtcaa tttcgtctct gcttctttag ctgttcccca gtttggacca
     6421 tatccaatgg tgacgaaacg ctgtgctaaa cgaaataaac tttgatatcc atcaaaaaat
     6481 tcttccgttt tttgaataat cgctggtaaa tggttgatta ttttgcgcaa ctgttgcatg
     6541 tagtcttgtt ctattttttt gctgatttta tttttactta ttccaattcc tatccctaag
     6601 agtagtaact gtaaaacagt cgcagaatag ccttttgtaa cgaagcctac tgattctacc
     6661 ccggcaccta aatcaataat ttgtgcagct tctaaagcaa gtggactttg cacgtcattt
     6721 gtgatagcaa caacaggtaa cccttgtttt tgtatcattt ttactgcttc aacagtagaa
     6781 gcacttttcc cgctttgtga cacagcaatc acagtatcga ttgaaggaga aagttttcca
     6841 taattgaaaa aataaaaagg ttcttgaata tcgacagtaa gatcaccata agattccaat
     6901 gctggcttcg ctgctaaaca agcgttataa gatgaacccg ttgctaagat cagtaaattc
     6961 actgtttttt tcatatgttc tcttgtcgaa aaatcattct ttttcaagat cgtacttagt
     7021 acttcctttt cttcatagat ataatcttgc atcgtaaaca ttttctctgc tcctttttta
     7081 aaaaatccct atccacgaac caaaaattcc aaagaaagct agtccgatca agatcgttaa
     7141 tggtttcact tctttcttca atagataaaa cactatgcca aatgctccaa gacataagat
     7201 atttggaacg atatcatcaa aaatactttg aacagtcaca gcattttctc ctgaaccgat
     7261 ttttaaagga atattgatgg tgatcatcgt ggcagtcatt gctccaacga ccatcagtcc
     7321 aataatagac gcgcctaatg tcaaactttc catcataccg tttgcctgga tctttttcaa
     7381 aaaacccgtt cctaagcgat atccccaacc tgttgctaga tagcgaaaca gaatgtgcgg
     7441 aatattgaaa atcaaaatga acaagatagg gcctaaaata tttccttgca tggctaaaga
     7501 ggtacccaca cctgtggcaa tcaatcgcaa agttccccaa aagaaagaat ctcctaaccc
     7561 tgcaagaggt cccatcaaag atgttttgat actatcgatc gcagtcacat caaattcagg
     7621 atcatcctta ttttcttcct ccattgcagc cgtgattcct aaaatcagtg taacgatatg
     7681 tggggtcgtg ttaaaaaact ctagatgccg tttcaaagct gctgccattt cttctttcga
     7741 ctgatataac tttttcaaaa tcggaatcat tgcataaaca taagccatat tcatctgacg
     7801 ttcataattc caagaaaact ccatttgaaa agaacgccaa aatacgtgat ttagctcttt
     7861 tttcgttatt ttgctgtttt tgatctcaga attcttcatc ttcttctacc tcccctgcag
     7921 ttgtttgaag tggtagttga cggacattca taatattgac taccacaact gctaaaatcg
     7981 caccaaaaat agctactccg gtcacaggaa tctctaagta agaagctata gcaaaaccta
     8041 agaaaaaata tggtgcaact tgtttgttga tcagcaaacg agcaagcatc gcaaaaccaa
     8101 gagctggaat caaccctgta gctactgaca accccttttg aacgaagtca ggaatcatat
     8161 ttaatagatt tccgatcgta ttgcttccca ccatataaga aaccattacc acaacaccaa
     8221 gcatcaagga aagaccgata ccggcaagca agtgcatatg ctcgactcct ttataatttc
     8281 cttcttctgc ataattgtct gctttatggt tcataattgg aatcaaaagc cctaagtaaa
     8341 tatttttcaa tatcaatgtc aacgtagcaa tcggcaaacc tagtaaaagc gcggtttctg
     8401 taccggctcc agctgtaata gcaaaagccg tccctaaaat cccaccggtc acaacatcag
     8461 gtggaatcga agcacctact gagaacgatc cgataaatgc taactctaaa gttgcgccca
     8521 taatgatccc agtttttaca tcacctaaca cgattcctgt aaacagcccc gtcacaatcg
     8581 gacgagaaag caacgatgtc cctagtgcat attctgattg tgcgacaaat gcaacaattc
     8641 ctaataagac tgcttgtaca atcattgagg ttcttccttt cttacaaaac ttctcttaca
     8701 tccacttttt tatctgctgg tacttgacgg atttctactt ctatttgttt gtcaattaat
     8761 cgtttcaagc gttcctcctc ttctggtaaa atatttaccg cttttccgat gctgcgagta
     8821 ccaggttttg ccttgatccc tcctaagttg atggttttga tttctggata cgcttctgcc
     8881 agcttatatg catcctcgat cgattccact acgatgaata acttatattt atctgtcacc
     8941 ccgcttttca acgcggcaat cgagtcatca attgatttga tcactagttt gactccttgt
     9001 ggcttggcta atttgatcgt tgtctttcgt atctcattcg taggtacatc atcattagca
     9061 atcaaaatac agtctgcgcc tattgtttgt gtccatgaaa aagctacttg tccgtgtaat
     9121 aaccgatggt cgactcttgt caatacaatc atttttcttc ctcctaaaat tcgtcttctt
     9181 ctattttctg attgagccgc tcgttacaaa actggatcat tttttttgaa tcagttagca
     9241 cctgctggat caaaacagtt gtctctactt cagcgtccaa ccgagatact agctcaatca
     9301 atagtggcaa attcaatccg gcaacgagat agacatgatc ccgattgata tattgtaaga
     9361 attcgttgtt gacacttccg ccaaaaagat ccgtcagaac aatgatttct ttatctttgt
     9421 acctcataaa aagctgcctt actgtttccg ctaaatcaaa attctcatca gtatagcaat
     9481 cgaatacagt aatagaatga tttttcccat agatcagctc taatgaatct aatatccctg
     9541 aagcaaattt tccatgggaa gctaagataa tttctctatc cactttttca tctcctcttt
     9601 tcacttacac atactaaaaa agcaagttgc atgccaaaat tagtggcaac aacttgctta
     9661 tgttcaaaat tcttcttcta tcgagagaaa ctctgtatct tcactcaaaa tatcatagat
     9721 ataggcaatc tcagaattgg ggatcatgac actataatgc tcttcaatga cactaaacgc
     9781 agatttaata gcagataacc cttctttttg acactgcttc catttctcta atccttgata
     9841 ggtttcgatt ggcatttgtc ggatcaatcg ttcaatgaga cagctcacat ggacatacaa
     9901 cgctaatttt ttgggattgc tgatgcttcg attcaactgc acctctaatt ctctcataaa
     9961 atgatcgatt tctcccatca ccttctctgt atctagaata gtcacggaat ccagtacttt
    10021 ttccaatgaa aaattacgaa tgatgcgctg attgaatatt tccttctctg aagcattcat
    10081 agtagtgtcc aaccattcag ctagtacaga catcttttcc cccgagatga ggtcttctaa
    10141 tgaaagatag tcgatattgg tgatatcagg atctgtggtg ccaatcattc ctacgacttc
    10201 gtatatggaa aataatgggt cactgttttt cttatctttt aattgctgat attcatatgg
    10261 gatgattttc aactgacaag ccgtaggtaa gcttttttct agcagttcac ttaagtggac
    10321 cgccgtaccg attccagtga agcaagtagt aatgagtgct ttgggtttgt tttcctctgg
    10381 atagagcagt cgccattctg gctggatctg tcgaacagtc tcttctgcta tctcttctaa
    10441 tgatacattt ttttgcaggc attcccctac tgtgattgct aaaggtgtcg tcacattatt
    10501 caaaatcaaa agcggtacgg ataactgttt tttaaaaaac tgatggattt ctttcagaga
    10561 tcccatgtct actaatatga tcaatccatt tgaaacatcg ctgttttcgc aataatcaag
    10621 gatttctgaa gcaatctgct gtggtgtgac atctagtggc atatcaaatg attcaaatag
    10681 atcctcttgt aataaacgat ttactacatt tgccatactg ctagcagttg cataaccgtg
    10741 agcagcaatc atcgcttttg gcagtcgttt ttcttttgac catttcgctt tttttagata
    10801 gatcgtcagt aaaatccgat ccatacttgt gtcttcaata tctaatttga tcttacttaa
    10861 ttccagaatt cgttggacat agtgataact agatggataa gactgagaaa cttgttgttc
    10921 taattctttg attagttgga taatatcatg ttcttccggt aaccatttga tagtgcttcg
    10981 ctgatagaga tagtaactca aggcatagac actgtttcca tcgaatttga tttgataggc
    11041 agtttccatt tgtcgaaagg tctctcggat agattgtgtc caataaacca gcagctcgtg
    11101 tttttcttgt ctactggtct caaacagcaa aaaatcaaat aattgaatca cttcttgctt
    11161 taattttcct tcgcaagaat atagcagaca gttatttttc ttgaaatcaa ttaggatttg
    11221 ctcaaaactt ttaacgattc gctgctgttc gggctgttgc ttctccatta attgctctaa
    11281 ggtctggtct tgcgtaatat agattccttg ctgtgctgta tttttagtaa taggctgcgc
    11341 aagtagttct ttttgcaaat gatggatcgt gacatggatt tcaggctgtt cacgctgttc
    11401 agcatatgct tttgctgctg aaactttgac gatatttttc agctctccta tgtttccgga
    11461 taatttctga cttgttaaaa gtgacaaagc tggtccactg actgacaaag ggctccccat
    11521 ttttctttgt tcttcgagaa aaaaagagta aatcagttct agccgttcat tacgactacg
    11581 ctgattaagt gcaggtagtt cgattcggat aggtattctt cgaatgaaag ttgttaagaa
    11641 atgactagta atctcttcag tcgtagcaaa aaagagacgt gttttgatcc ggcgtgacaa
    11701 attaggttct cccatacgat aaatcacacc ttgatcaaga taggtgaaca gtttttcctg
    11761 accttctggt gttaagcgat gtacctcgtc taaaaacaag atcccaccat ctgctgcttc
    11821 aaaagcccct tttttatttt cttctgcacc agtgtacgct cccttaacat gtccaaacaa
    11881 attgctagtc aaaagttcag gattatctgc atattgtgca caattaaaag tgatgaatgg
    11941 tgcagaatct tctagcagat catgaagcag acaatactga tagaccaaat taactaaata
    12001 acttttacct gtgccacttt cgcctgtaat caaaagtggt aatccgccat ctggatagta
    12061 aagagctgtt ttgatttgtt cgatactttc tttcaaactg gcgtcgtatc cgatcataaa
    12121 agagaataag tcttttggtt gcgcgaaaaa aggctgttcc tctttgagat catcgataga
    12181 aaaataaaaa ttcgtgcgta acttaaaaaa ttgtttttca aaagcttctt tgtggaaata
    12241 aatcactggt cgagaattga ttttgactag tagttgctca tcatttaact ggttcaaata
    12301 atgactgact gtgtttcttt ttaccccaaa atgatcagct aacctttgtg ctgtaaagac
    12361 ctcgttcata tgttctaaat cgaaaaaggc tgtctggttc tctaagtaac ataaaatttc
    12421 cttcttcaac aacttattca cccctcctcc atcatagaaa acggattcat tttgtcaata
    12481 aaatctttaa taaagaattg aaaaatatat cttattatag acagcagcct acttatccct
    12541 tataattttt attctaaaaa acgtacagtt ccttccagtt tttatactgg aatttcaaca
    12601 tatgaaagaa taaggaggct atgactattc aatgaaatcg gataaatata aaaacaggaa
    12661 acatacttcc tggaaaactt ttcgagaaat catcggatct tatctcgctg tagttgtatt
    12721 tttgcctttg ctttatatgg ctatgacaga acaaactcgt tttattggcg tagatacgca
    12781 atatatagcc gaaacaggat ttttattcat ctttatcttg atttttgcgt tctataaatc
    12841 cttccataca aaataagatt cacagatttc aactttcctg tctgttttta gctattacaa
    12901 atccttatat gtggtataac aacaatttat tggcataatg aggaaaaacg agttatgatg
    12961 ctttcagaga gtaaagaggt actgttgtca ccatcctttt tattgattac gtacaccact
    13021 cttagttttt tcccattgat tacgaaaaca aggtagaaac attgatgttc ctaccttgtt
    13081 ttcttttttt attttcagat caaaatctca agtagctttt gacttgatgt cgggaggttg
    13141 tcttcgttcc atcaaacctg atagaatctg cgccaaacca tagttttctc cactattatt
    13201 tattcctcta agatagtacc cacaataaac gaataaatat ttttctcatt ttcatactcc
    13261 ttctttgaaa atcaattaga tttctctcaa cccacttttt tacaaaaatt acgtagcaaa
    13321 aaagattgca agaacaaatt aaaaaagaag taaatagagt ttcatctata aacatttact
    13381 tgatatattt tttatgcaga attcatttat agaaaaatta agtaaaatag acgttcctat
    13441 gaatgaagag actggcatgt tttctcattt tctttttttt aaaaaaaata gtctattcaa
    13501 tttctaaagc ttcttcataa taaaactttc atttatgaaa aggcttgttc tcttttcatt
    13561 ctaaatttcc taagctacaa tgtggtagta cttgtttttt caattgtgga caaataaata
    13621 ataggaggaa tcatatgatt cagttattac gttatgcaaa agactatcgt aagcaaatca
    13681 ttttgggccc gttcttcaaa tttcttgaag ctgtcttcga acttgtcctg cctttaatga
    13741 tggcttcctt gatcgataat ggactaaaaa tgaatgaccg aggaaaaatc attgaaatgg
    13801 gcttatggat ggtcgctatg tctgtgattg gtctgatttg tgctatcatt tgccaatact
    13861 atgcttcagt agcttcacaa ggatttggta cagagttacg taatcagcta atcaaaaaaa
    13921 tcaatacttt ctctcacaac gagttgaatc attttggaac agataccttg atcacccgaa
    13981 tgacaaacga catcaatcag cttcagctag ctttagcaat ggtcattcgt ttattgatca
    14041 gagcaccgtt tttaagtatc gggtcagtgg tcatggcttt tgtgatcgac tgggaagtag
    14101 gtctgttttt cttagcatta ttgcctattt tctcaatcat tttatttttt atcatcaaaa
    14161 aaaccgttcc tctctaccaa aaagtgcaag aaaaactaga tcagctcaat gaacacgtta
    14221 gtcagaatct tagcggtgtt cgtgtcatcc gcgcatttgc gcagacaaaa aaagaaacag
    14281 agaagttcga taaaagcaca aatgatctag cgaacaatta tttacgtgtt tccaatatct
    14341 ctgccctatt atcacctgct acaaccttga tcatgaatgt cgggatcatc tgtttgctga
    14401 cagttggcgg gatcaaagta aatattggta gcttgcaaca aggacaagtt cttgctttga
    14461 tcaattatat gaatcaaatg cttttggcac tgatcgttgt ttcaaacctc gtcgtgatct
    14521 tcacacgtgc cgaagcttca ggaaatcggg tgaaagaggt gctagacaca gaaaattcga
    14581 ttcttgaagc tgaggtcagt actactcccg atttttcttc tgaagcaatt atcgcttttg
    14641 accatgttga cttccgttat actcctgatt ctggtctttc tctccaaaat attacatttc
    14701 agctgaagcg aaatacagtc cttggaatca ttggtccaac cggaagcggg aaaacgactt
    14761 tgacacaatt gattccgcgt ttctatgatg tcagttccgg tacagttata atggacggaa
    14821 tggatgttcg ttcttggcag ctggatcatc tccgtcagca aattgctctt gttccccaaa
    14881 cttctgtact atttaccggg actattcgtg aaaaccttca atggggtaag aaagacgcca
    14941 cagaaaaaga atgttgggaa gctctgaaaa tcgctcaggc agaagaattt gtccgtcagc
    15001 ttccaaatgg tttagataca aaagtcatgg aaaatgggaa aaatttctct ggcggacaaa
    15061 aacagcgact gacgattgca agagctttga ttgccaagcc agcattactg attttagatg
    15121 actctttaag tgcgttggat tatcaaacag acttggattt gcgtaaggcg ctacagactt
    15181 atcttggcag tacagtgatt attatctccc aacgtgtccg ctctattcaa gaagcacatc
    15241 atatcttagt gatggatcaa ggtaagattg ctgctcaggg tacccatgaa gaattacttg
    15301 ctcaatcagc agaatatcag gaaatcgtcg catcacagga ggaagaatag ttatgaaagt
    15361 aaaaaaatta tccttgcaga cgctccgccg attcattcct taccttactg cctatgcaaa
    15421 agacatttgg ctagctgtga tactaggaat catcaatggg attgcaacag ttgtgatgac
    15481 gttacaaatt ggtcaaagta tcgatcaaat gattggagaa ggacaggtcc gcttcaatcg
    15541 actgttccat ttacttattc tattcggtgg tatcgtttta atcaacgtag tcagtcaatg
    15601 gctcatccaa gtgttaagca atcggttagc ttacttatcc gttgcaaaat taagaaaaga
    15661 tgcatttgct catctgaatc gattgccact tcgttattat gatcaaaact cccacggaaa
    15721 tatcgtcagt cgatttacaa atgatatgga caatatttcc attgcaattt ctgcggcctt
    15781 caatcaatta ttttctggag tggcaatcat cattctttcg tttattttta tgatgcgtct
    15841 aagtccactt ctgacattgg tcgttctttg ttctacacca attattttcc ttgtgagttg
    15901 gatcgttgct cgtgcttcac aaacaaattt cgatagacag caaaggatta ttggggatat
    15961 ctcgggcttt gtgaccgaac gaatcggtaa ccagaaaatc gtgaaagctt ttcagcaaga
    16021 agaagctagt caacagcaat ttgaactgct aaatgctgat ctatatgaaa aaggacaacg
    16081 ggcacaattt tcttcttcct tgacaaaccc gtcttctcgt tttgtagatc accttgctta
    16141 tttatcgatt ggtttagttg gcggcctatt ggcattgaag agtggttcgt caatcactgt
    16201 tggtgtgatt tccagcttta cgatctactc aagtcagttt tctaaaccat ttatcgaact
    16261 ttctggaatc acgacacaac tgcaaaccgc tttttctggt ctagaacgag catttgattt
    16321 gatcgatcag ccagaagaaa agcctgattt gccagatgcc tatgccttgg aacccaaaaa
    16381 tatcaaagga aaagtctctt ttgatcatgt ttctttttct tatgacccca agcagcgttt
    16441 gatcgaagat ttcaatttta ctgcttatcc aggtcaaacc attgctatag taggaaaaac
    16501 aggggccgga aaatccacac ttgttaattt gctgatgcgt ttttatgaca caacaaaagg
    16561 aacaattaca gtcgatgacc atgacatccg ctctatccaa cgtgatacat tacgtaaaag
    16621 ttttggaatg gtgttacaag atacttggct atttgacagt acattgcgag aaaatcttcg
    16681 ttttgggaat ccggaagcaa gtgacgcaga aatcaatgat gccctaaaag cctcttatat
    16741 gtatgaattt gtagaacgtt tgccggaaaa attagatact aagattggcg gtcaagggtt
    16801 aaagatatca gatggacaac gccaactgtt gacgattgct cgaacgatga tcagtaatcc
    16861 ccctatgttg atcttagatg aagctaccag ctcagtcgat acattgacag agaaaaaaat
    16921 ccaacaagcc ttcttagcaa tgatggaagg aaaaaccagt tttgtgattg cccatcgcct
    16981 gtccacaatc aaaaatgcgg accagatatt agtgatggat cagggagcta ttgtcgaaat
    17041 gggcacccat gatgagctgc ttcaaaaaga tggttattac cgacggctct atcaagcaca
    17101 attcacagca caataaaaac aagcagtctt ctgggatggg aagactgctt gttttttatt
    17161 tagcgtggat cgctaaactt cttaaaattt ttttgtaagg aatactacgt cctacgtaat
    17221 aaatggcaat tgattgaatc ggtaaccata ttactgtttt taacaggcgg ggcgcccaaa
    17281 cgacccagct gaacaatggc acatggtaca tcaacgcaag ccataatggt gtcaaaaata
    17341 agttgatgat cagcgtgaca ctaagcgtag ccagaatggc atttttccaa gtgatctctt
    17401 ttcgatagaa aaagaatccg tagattgctc cttcaataaa tgcattcaat gtaaatccaa
    17461 taaagaatgc agatttcgag aagagggcca ttcctactaa atcagctaaa actgcgccta
    17521 tccctgacca aaaaggacca aaaagaatgc ccatgatcac ttgtggaata aatgtgacac
    17581 tgattttaat aaatggggtc tcgattgcta ccagtcggga caaagtgacc atcaatgcaa
    17641 tcaataagcc cataatagca atcatacgtg cgtctaaccg gtgtttcttc atttttagca
    17701 tctcctttaa gagctgctac atcgtatcat gcagcgaatg cgaaaataaa accgcgaaca
    17761 ttacgtcctt cgtccaagag gcaacatccc atcctcttag cacttaacgc tttattttct
    17821 actctgtatt atttttggga tcttttccca cagaaactat agcatatttt cacatttact
    17881 gcaaatacga aaaactaata ttattgttta tattatccaa tacgttcgta ttatatcgca
    17941 tattatcagt ttgtttagct tttatagcta catataggcg aacattaaac gttatttttc
    18001 catacgctta tccatcaaaa tgaaagtgga tgcttgtcta tttcatggac gattcttcga
    18061 aaatctagga agccggcttc cagtcctctt agtagcacct cagctgttcc gatattcgta
    18121 gctaacggaa tctcatagac atcagataaa cggataagtg ctgttacatc tggttcatgg
    18181 ggctgtgcag ccagcgggtc tcgaagaaaa atcaccaaat ctaactgatc ctccgaaatc
    18241 atggctccaa tttgctgatc cccgcctaac ggtccggatt taaaccgatg gatcggcaag
    18301 ccggttgctt ccgtgatcct ctgcccagtc gtcccggtag caaaaagttc atgtttttcc
    18361 aatattgttt tatacgctgt tgctaatttc accatcatag gtttttttcg gtcatgagcg
    18421 atcaaagcga ttttcataag agacactcct tttgatattg ttaacatact tatttactcc
    18481 agtgtatcag tttttatttc ttatggatat ttattaaagc ttaaaactag cacattgaca
    18541 aaaaaaagaa aaaattcttg ttcaacatga cattgaagtt agttaacgct ttcatttata
    18601 ctttaaatag ttaataaaac taaaaaagaa agtgtggaac gtattatggt agaaatggct
    18661 ctgaaaaacg tatataaaaa atatgacaat gcagaaaatt attctgtaac tgattttaat
    18721 ctatcaatca aagaccgtga atttatcgtt tttgttggtc cttccggttg tggaaagtca
    18781 actactttgc gaatgatcgc gggactggaa gatatctctg aaggagaact aaatatcggc
    18841 ggaaaagtca tgaatgacgt ggcacctaaa gaccgtgata ttgccatggt tttccaaaac
    18901 tacgcattgt atcctcatat gactgttttt gataatatgg cttttggctt gaaattacgt
    18961 aaatatgata aagcagacat caaaaaacgt gtggacaatg cagcagagat cttaggtctg
    19021 acagaatact tgaaacgaaa accagctgct ttatctggtg gacaacgcca acgtgtggca
    19081 ttgggacggg caatcgttcg ggatgcaaaa gtcttcttaa tggatgagcc attatcaaat
    19141 ttagatgcta aattacgtgt agccatgcgt gctgagattg ccaaacttca ccagcgtctt
    19201 gaaacaacga ccatctatgt tactcacgat cagacagaag caatgactat ggctgaccga
    19261 atcgttatca tgaaagacgg atttatccaa caaatcggta caccgaaaga agtttatgat
    19321 acacccgtca atatgttcgt tgccggcttt attggatctc ctgccatgaa cttctttaat
    19381 gtgacattga aaaatggtgt tatcacagat ggacatgggc ttaagcttcg tattcctgaa
    19441 ggaaaaaaca aagtattagt tgaaaaaggt tatgaaggaa aagaagttat cttcggtatt
    19501 cgtccagaag acatccacag tgaacaagta gcccttgaag cttcacctga agccgtagtg
    19561 aaatcagaag ttgtcgtttc cgaattactt ggagctgaaa cgatgcttta cacaaaaact
    19621 ggttctactg agttcatctc aaaagtagat gcaagagatt tccataaacc aggcgaaata
    19681 gtcgaccttg ccttcaacat cagcaaaggc cacttctttg ataaagaaac agaacaagtc
    19741 atcaaacttc cataaattgc cccctgttag agctgtgcca gaa
//
LOCUS       DASJPH010000052        19759 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJPH010000052 DASJPH010000000
VERSION     DASJPH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.052"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..1147
                     /locus_tag="U3995_001691"
     CDS             104..1147
                     /locus_tag="U3995_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1797015.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="U3995_001692"
     CDS             1320..1544
                     /locus_tag="U3995_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797016.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="U3995_001693"
     CDS             1547..1804
                     /locus_tag="U3995_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797017.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="U3995_001694"
     CDS             1906..2259
                     /locus_tag="U3995_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797018.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="U3995_001695"
     CDS             2312..3106
                     /locus_tag="U3995_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797019.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="U3995_001696"
     CDS             3135..4253
                     /locus_tag="U3995_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797020.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="U3995_001697"
     CDS             4286..4606
                     /locus_tag="U3995_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797021.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="U3995_001698"
     CDS             4861..5550
                     /locus_tag="U3995_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEN1797022.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5587..5754
                     /locus_tag="U3995_001699"
     CDS             5587..5754
                     /locus_tag="U3995_001699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797023.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="U3995_001700"
     CDS             5774..7402
                     /locus_tag="U3995_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797024.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="U3995_001701"
     CDS             7453..7824
                     /locus_tag="U3995_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797025.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="U3995_001702"
     CDS             7909..8109
                     /locus_tag="U3995_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797026.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="U3995_001703"
     CDS             8243..9469
                     /locus_tag="U3995_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797027.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..10461
                     /locus_tag="U3995_001704"
                     /pseudo
     CDS             10066..10461
                     /locus_tag="U3995_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            10539..11578
                     /locus_tag="U3995_001705"
                     /pseudo
     CDS             10539..11578
                     /locus_tag="U3995_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            11591..12529
                     /locus_tag="U3995_001706"
                     /pseudo
     CDS             11591..12529
                     /locus_tag="U3995_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            12522..12869
                     /locus_tag="U3995_001707"
     CDS             12522..12869
                     /locus_tag="U3995_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797028.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            13184..14041
                     /locus_tag="U3995_001708"
     CDS             13184..14041
                     /locus_tag="U3995_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1797029.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            14061..14621
                     /locus_tag="U3995_001709"
     CDS             14061..14621
                     /locus_tag="U3995_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEN1797030.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15510..16277
                     /locus_tag="U3995_001710"
     CDS             15510..16277
                     /locus_tag="U3995_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEN1797031.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16303..16848
                     /locus_tag="U3995_001711"
     CDS             16303..16848
                     /locus_tag="U3995_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797032.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16849..17415
                     /locus_tag="U3995_001712"
     CDS             16849..17415
                     /locus_tag="U3995_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797033.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17430..17678
                     /locus_tag="U3995_001713"
     CDS             17430..17678
                     /locus_tag="U3995_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797034.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17697..17936
                     /locus_tag="U3995_001714"
     CDS             17697..17936
                     /locus_tag="U3995_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797035.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17953..18537
                     /locus_tag="U3995_001715"
     CDS             17953..18537
                     /locus_tag="U3995_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797036.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18644..18925
                     /locus_tag="U3995_001716"
     CDS             18644..18925
                     /locus_tag="U3995_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1797037.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18915..19256
                     /locus_tag="U3995_001717"
     CDS             18915..19256
                     /locus_tag="U3995_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1797038.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7490 a         2614 c         4036 g         5619 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
    10381 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
    10441 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
    10501 aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc
    10561 gtgaattgga aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg
    10621 cagtcaagtt ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg
    10681 acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag
    10741 tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg
    10801 tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga
    10861 cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag
    10921 gcgtggcgga cctccttcac cggctaggac taagctggac ccgtccgacg tataccctca
    10981 aaaaagcgga ttccaagaaa caacaggtat ttttggaaga aaccttcccg agcctaaaaa
    11041 aaaattgctg aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga
    11101 ttaccaagcc attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta
    11161 tggacaacac aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat
    11221 ttgtacagaa gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt
    11281 gcgggaatac cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc
    11341 caagctgatc cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc
    11401 tccctacagc cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt
    11461 catcaacaat gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt
    11521 agccgatgta aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag
    11581 tatttaattc aacctatata gttcaggaac aagaagcgag agaagaagag aattagctaa
    11641 aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga
    11701 taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat
    11761 agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga
    11821 cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat
    11881 agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact
    11941 ccagttatat ttccatacca atggaattga tcgtacggat attgctattc caccagaacc
    12001 aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga
    12061 agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca
    12121 caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc
    12181 aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta
    12241 ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac
    12301 ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa
    12361 aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt
    12421 tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac
    12481 tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata
    12541 gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag
    12601 acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg
    12661 cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac
    12721 gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc
    12781 ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct
    12841 cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat
    12901 tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt
    12961 ggggattgat tgcttttgtt tctatattga atccggagca ggataccata agaacagata
    13021 ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt tctccaaata
    13081 ctttcaatta ttgtgaaata tttcccttta attaatcaaa ttttttataa aagtaatata
    13141 taaaataaat tgcttgaagt attatattta gatatcataa tttatggaag aaaaattaat
    13201 taaaaaggat gagattataa tggggttact ggttccgaga gctaatactt atactatacc
    13261 tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg attactagag ccatcaatag
    13321 agagttttgg aattctgaaa gtgatactgc tcacagttta tacgtaggtt cttatgggag
    13381 aggaacggca attagtacta gtgatataga tataatagtt gaattaccga tggcagaatt
    13441 tgacagattc aaaaattatt tatcaaatgg cccgtctaag ttattacaag ttataaaaaa
    13501 cgcatttcag gaaatactac caaatagtga tattagagct gatggacaag ttgttaaaat
    13561 aaatttccat gatggaatca aatttgaaat agttccagct tttaatgaaa aagattattg
    13621 gggtgagtca aaagggttta tttatccaga ttcaaacatg ggagggaact ggaaagctac
    13681 aaatcccaaa aaagagcaag aagccatgaa attaaagaat acaaaaagta ataatttatt
    13741 atatgctacg tgtaaacatt ttcgacatgt acgagataca gaatttacga gttatcatct
    13801 ttcaggaata gtaattgaca gtttcgtata tgaggctatg ggaaattgga aattcgttga
    13861 aaataacagt gggggtcaaa acattagttc tgtatcttat gagacagctt tattagaata
    13921 ctataactca cataaagtta tgggcggttt aaatttatac tctcctggaa gcaatcaatt
    13981 tgttaattct gatagtagta tcatatgttt agaaaaagta ttaaaaaaaa tagctttata
    14041 aagttttatg gaggaacaac atgggagacg aaaaaaagcg cttggaagtt caattacgtg
    14101 aagcatatgg acgagtaacg tatacttaca ccactcattt aaagcaagcc gataatatta
    14161 tttctgacaa taaaatttta aggtgtcttc aaattggttt gtcagccctt actactggag
    14221 gggtacttag taccattatt actgataata gtgagttaac ctttgttgct ggattatgtt
    14281 caacaatttc tcttggatta aacttatact ttaagaattt tgatcttgct gaagaagcta
    14341 aaaatcatca aattgttgcc gataaattat ggttgatcag agaagaatac gtaagcttac
    14401 taacagattt aaatcgttta agtgttgaag atattgtgaa aaaaagagat caattaatga
    14461 acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa tagtaaaagc tatgaatcag
    14521 ctcagaaagc attaaaatct gaagaagagc aattttttac agaagaggaa ttggataaga
    14581 tgttaccaga gcatcttaga aaaaagagtt ctaaatattg aactcttttt ttatgcttaa
    14641 aattctcttg taatttttct attcatctat atttaagaag aaatatgatg atagaaacat
    14701 ctcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
    14761 catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagccatt gcctagtccg
    14821 acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc aagccgttct
    14881 aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag
    14941 ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg
    15001 tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg
    15061 tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc
    15121 tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc
    15181 attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga
    15241 ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa
    15301 acacttgtat taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca
    15361 ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg
    15421 atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca
    15481 aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag
    15541 aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac
    15601 ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag
    15661 cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta
    15721 tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg
    15781 gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat
    15841 tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata
    15901 gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta
    15961 gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag
    16021 ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt caactagctt
    16081 tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc
    16141 caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta
    16201 ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag
    16261 aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa
    16321 gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc gattcattat
    16381 cagtgggggt atggtcgata tatgctgatg gacgtattga atcaaggaat ttatttaatc
    16441 aataaagcat acgacttacg agataaatca agagaagaat tagcaaaagg aatctttgat
    16501 agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa
    16561 acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt
    16621 tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt
    16681 ctagatgata aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac
    16741 aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta
    16801 ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa
    16861 agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg
    16921 attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac
    16981 tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag
    17041 aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca
    17101 acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta
    17161 gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac
    17221 gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg
    17281 gaaacagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat
    17341 gatgaaattg aagattggtg tgtgtggtta gtagggacag atgattgcgt tgcatattac
    17401 acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttata gatcatgaag
    17461 atttgattga ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta
    17521 atgcctttta cgaaagaaat tatgcagtct atgtccttta tgatgacaat acagaaagcc
    17581 ttgttgatga agagtataaa ttggatttag agaatgtttt atatgggata gaaaaagaag
    17641 atttagcaaa atatattttt tcatggttgg gtcaataaac gaaagagggt agcaacatga
    17701 caaaattcaa aagtaaaggt atgcaatggt gttcttatta ttatgttggg aatgatgaag
    17761 agcttttgag aaaagccaaa caaaaaggtt atccagataa gatgttaaaa cagattggct
    17821 attacatgcc agaagagaga atagaaaaag aatcttgtaa ggttcttttt gatagtgaac
    17881 agctattagt tttgttaact caaaataaat tgatttgtca atttgaaaat aattaatatg
    17941 gggtgaagaa ccatgaatga gaataagatg gtattaagtg aatacgcaag attgagaacc
    18001 attaattggt tgaatggtat gcaaagtagt ggcgcttttg atggatttga tgaggagaat
    18061 atggactttg aaagtttatc gaatgaggat attagatatt attttatcga atggtatttt
    18121 acagaaccag atgaagaggt ggaagatgaa ccagaattaa ttttagaacg tcaaagaatt
    18181 ttattcagtg aacaagatag taagaggttg tatggtttat atgacttaca actgaaagga
    18241 ttaaaagcgc agtatgttgc tgatagttac gatgatatgg tagaacaggg ggcagatagg
    18301 gttgtcactt attcagaaga tgatgaagag gaatgggaaa actattcaga tgatgagata
    18361 tgctttaatg atgggtatat gcctattgaa atcaccgaag aagaatatga taaagtgtcg
    18421 gatagcgatg agattggtat gtgtacggtt gtaagtggat tggatataga tgtccaaaga
    18481 gtagaggttg cataccagaa gatgaaagaa cgtataaaaa gtaaagtaga atcttgattt
    18541 gattggattg tttgcaaagt agctaattaa gagtataata taatcataaa tgtagttaat
    18601 ggatataata atgagtatat tatactcaaa agaggtgaac gaaatgaagg taaaaaatcc
    18661 aaaagaaaaa agcagaattc aagtcggaat agacaaggat ttaaaagaaa acgcagaaat
    18721 gatattggaa gagctagggt taaatccaac aacagcaatc acaatccttt ataagcaagt
    18781 ggtcgctaga ggtgagtttc cagtagagat aaagctaagc gaagaggaaa agcaaggaat
    18841 cagattgcaa cagctaacta tggatatgcc agttgatgtt ctagatacag atgaaaagtt
    18901 agaagagtgg ttcaatgaag cctaatgaga tagtaacggt ctatgttgca tttacagata
    18961 aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat
    19021 tttttggaat cacaagtcaa tatgagaata aatcagacca aataaagaga gtgtattttc
    19081 ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc atggattgac gtagggtctt
    19141 taaaggctat tcccaaaagc cgagagaaca tatcatttaa aaaggttggt atgctatcag
    19201 ctattgatac gaaaaagcta aataggttta tcgagagatt acaaagaaaa gtctaacaag
    19261 gcttttcttt ttttgtttat atgtatatta agatatacgt ttatataaac gtatttatgt
    19321 atattaaaat acacgtaaat atatacgtga ttataaaaat taagaaaata ttaagtattg
    19381 ttaaatagtg ataattagta atatacgaat tataagatac attttatatt taatgtatgt
    19441 aaataaataa aatgactgac ggatattgaa tagtaataaa taacaataat tcagacggat
    19501 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggta gtttcattag
    19561 agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact ggcaagttac atagagagag
    19621 tttgcttgca aacgaacgtt acccacttga atagaaaaca aaaataacat attagtgaat
    19681 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
    19741 gacgaacgaa cggtcgcgt
//
LOCUS       DASJPH010000053        19743 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJPH010000053 DASJPH010000000
VERSION     DASJPH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.053"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..176)
                     /locus_tag="U3995_001718"
     CDS             complement(24..176)
                     /locus_tag="U3995_001718"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1797039.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(367..1245)
                     /locus_tag="U3995_001719"
     CDS             complement(367..1245)
                     /locus_tag="U3995_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1797040.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1284..2402)
                     /locus_tag="U3995_001720"
     CDS             complement(1284..2402)
                     /locus_tag="U3995_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1797041.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2399..4498)
                     /locus_tag="U3995_001721"
     CDS             complement(2399..4498)
                     /locus_tag="U3995_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1797042.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4549..5370)
                     /locus_tag="U3995_001722"
     CDS             complement(4549..5370)
                     /locus_tag="U3995_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797043.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5367..6176)
                     /locus_tag="U3995_001723"
     CDS             complement(5367..6176)
                     /locus_tag="U3995_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797044.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6197..6691)
                     /locus_tag="U3995_001724"
     CDS             complement(6197..6691)
                     /locus_tag="U3995_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797045.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6703..7191)
                     /locus_tag="U3995_001725"
     CDS             complement(6703..7191)
                     /locus_tag="U3995_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797046.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7268..9964)
                     /locus_tag="U3995_001726"
     CDS             complement(7268..9964)
                     /locus_tag="U3995_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797047.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10392..11465)
                     /locus_tag="U3995_001727"
     CDS             complement(10392..11465)
                     /locus_tag="U3995_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1797048.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11462..12289)
                     /locus_tag="U3995_001728"
     CDS             complement(11462..12289)
                     /locus_tag="U3995_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797049.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12290..13096)
                     /locus_tag="U3995_001729"
     CDS             complement(12290..13096)
                     /locus_tag="U3995_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797050.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13108..14193)
                     /locus_tag="U3995_001730"
     CDS             complement(13108..14193)
                     /locus_tag="U3995_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797051.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14210..14752)
                     /locus_tag="U3995_001731"
     CDS             complement(14210..14752)
                     /locus_tag="U3995_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797052.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15010..15999
                     /locus_tag="U3995_001732"
     CDS             15010..15999
                     /locus_tag="U3995_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1797053.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15996..16733
                     /locus_tag="U3995_001733"
     CDS             15996..16733
                     /locus_tag="U3995_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1797054.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16730..17281
                     /gene="coaC"
                     /locus_tag="U3995_001734"
     CDS             16730..17281
                     /gene="coaC"
                     /locus_tag="U3995_001734"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1797055.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17297..17929
                     /locus_tag="U3995_001735"
     CDS             17297..17929
                     /locus_tag="U3995_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1797056.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18014..18661
                     /locus_tag="U3995_001736"
     CDS             18014..18661
                     /locus_tag="U3995_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1797057.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18666..19649
                     /locus_tag="U3995_001737"
     CDS             18666..19649
                     /locus_tag="U3995_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1797058.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6060 a         4238 c         3442 g         6003 t
ORIGIN      
        1 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
       61 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      121 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      181 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      241 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      301 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      361 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      421 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      481 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      541 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      601 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      661 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      721 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      781 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      841 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      901 atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata
      961 ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat
     1021 atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat
     1081 gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac
     1141 tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc
     1201 acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct
     1261 tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt
     1321 ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc
     1381 cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg
     1441 gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg
     1501 tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct
     1561 ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt
     1621 ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt
     1681 gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc
     1741 caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc
     1801 tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa
     1861 tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta
     1921 taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt
     1981 tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac
     2041 gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca
     2101 acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa
     2161 ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca
     2221 gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg
     2281 cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt
     2341 tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc
     2401 atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg
     2461 tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac
     2521 attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat
     2581 caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca
     2641 ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga
     2701 cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg
     2761 ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc agctcgctca
     2821 ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt
     2881 tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat
     2941 ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt
     3001 ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta
     3061 acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat
     3121 caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa
     3181 ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta
     3241 gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg
     3301 tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt
     3361 cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct
     3421 gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta
     3481 acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag
     3541 gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa
     3601 acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag
     3661 gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa
     3721 agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct
     3781 gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt
     3841 cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg
     3901 ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct
     3961 gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt
     4021 gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta
     4081 tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca
     4141 aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa
     4201 tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc
     4261 aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg
     4321 tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct
     4381 ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt
     4441 ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct
     4501 tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc
     4561 aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac
     4621 ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa
     4681 aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa
     4741 aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat
     4801 actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt
     4861 agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt
     4921 gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc
     4981 tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa
     5041 tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc
     5101 agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt
     5161 cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta
     5221 gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg
     5281 attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc
     5341 atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc
     5401 gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga
     5461 tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt
     5521 aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc
     5581 caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt
     5641 aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca
     5701 aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag
     5761 aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca
     5821 gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct
     5881 agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct
     5941 gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca
     6001 cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc
     6061 aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt
     6121 tcatgtccag caactccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt
     6181 tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg
     6241 ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa
     6301 tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg
     6361 tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa
     6421 cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg
     6481 gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg
     6541 gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta
     6601 ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta
     6661 atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc
     6721 ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt
     6781 taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag
     6841 aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt
     6901 ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg
     6961 ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata
     7021 agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa
     7081 gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt
     7141 ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat
     7201 cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt
     7261 agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa agctcataag
     7321 cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta gaaacataat
     7381 aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt tctgtccgag
     7441 ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt tttacacggc
     7501 catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc acttcactca
     7561 tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac gaaaagaatt
     7621 ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga ctcaaaatat
     7681 gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata ttaggaattg
     7741 aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca aaataagttt
     7801 catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg
     7861 attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg
     7921 acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat tgaacattga
     7981 ttgaatactt gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct
     8041 tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt aactcccctt
     8101 ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct acgatcagca
     8161 ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg acaatctctg
     8221 acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg cataattgat
     8281 tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac aggcccttca
     8341 gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt cttagaaagt
     8401 tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat aagtaagtcg
     8461 tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc ttatagcttg
     8521 gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc gtcaatccgt
     8581 acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc aattttctac
     8641 tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact tcattcttta
     8701 tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt ttttccaaaa
     8761 tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt ttctttatca
     8821 ataaactagc aatcggcaag ctttctactt cggattccag catatttaac tgaatcaaga
     8881 tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca tccgcacaac
     8941 tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga gataacatta
     9001 aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct tcattttgaa
     9061 aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa gttggcaaca
     9121 taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca cctttttccg
     9181 tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca ccgactggac
     9241 gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc gataggcgat
     9301 ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca tcggtatcct
     9361 tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa agcagttcgg
     9421 gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt tcaataattt
     9481 gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca acacccgtgg
     9541 ctccatttat taatacactg agtccgttag gcggatattt gacagcggct atacattttt
     9601 gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct tcgtttattt
     9661 gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca ggctttcccg
     9721 gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg gcattctgac
     9781 gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata ttccctgtta
     9841 gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt tcctccagtt
     9901 taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta tatttgtatt
     9961 ccaattttta ctttgaaaca caaatagtcg aatatttgcc tcagatatct gacaaaataa
    10021 tttatctacc aatttagttt ttatctaata tgatttttat gttttttcat agctacaaat
    10081 attgaactgg attcatatat tagttttatt aatagattac agaagggtta agtatatttg
    10141 catagtttcc aattacttta tccttctgta actttttcaa tcttagattt actagcccgt
    10201 ctcgggccct attttacaat ggttttgtcc acttttctct ggtagaaaaa cgtggtagaa
    10261 aaaataaaat aacacaaaaa aagcggcgtt tcatccgctt ttggtagaag attggaaaac
    10321 tcagatagaa aaaggacgat ttttagctct ttagaacgtt gataagtccc cattcttact
    10381 gagttttgcc attatctcct atacatcttg aattctaaga ataggtcatt atagatgcct
    10441 aggtatttcg atcctagatc tttgtagact tcaagatggg agatcgtgtc atcagatgga
    10501 tagaattgtt cgtccgacga aatgtctttt ggcaataacg cttttgcttc ttcatttggt
    10561 gtagaataac caatatactc tgcattttgt gccgcatttt ccggttctag cataaagttg
    10621 atgaaatcat aggcaccttc tttatttttc acggtttttg gcataacgat attatcaaac
    10681 caaagattgg atccttctgg tgggatcaca taatgtaaat gttcattttc ccacatcata
    10741 tcggcagctt cgccagaaaa tgtcactgcc acggaggctt cttcattgat catatacatt
    10801 ttgatctcat ctgccacgat tgctttgaca tttggtgtta aatgattcaa tttatttgct
    10861 gcttctgtca gctgtgccat attcttacta ttcaaagaat agcctaaact attcaaagaa
    10921 agccccataa cttctcgtgc accgtcaatc agcatcaaac tgtcttttaa ttccggcttc
    10981 cacaaatcat cccaatgctt aatttgattt gcatcaaaga atttatcgtt atatacgatc
    11041 cctaaagttc cccaaaaata tggaatcgaa tactgattat ctggatcaaa atccaagtct
    11101 aaaaatcgtg gatcaatatt atttaatcct ttgatcttcg aatgatctaa tggaagtact
    11161 agtttttctt tgatcatttt ttggatcata tattcacttg gtatagcgat gtcgtagttt
    11221 gtcccacctt gctggatttt tgtgaacatc gcttcattag aatcaaatgt ttcataattc
    11281 actttgtatc catattcttt ttcaaatttc gtgatcaaac taggatcgat ataatctccc
    11341 cagttataga tggtaagtac ctttgctcca ctcatgccac ttgttttttc aagttgacga
    11401 acaccaagca gcagcaccgc aataatgatg agaataccta aaaacagaga ttgtaatttt
    11461 ttcatcgcag ccgtgccacc ccctcgcgta ctcttctgtt tttcttcagc cgttttggac
    11521 ggttttcttt actaataaag tagtatccaa tgaccaatat catggagaac aagaaaacta
    11581 acgcacttag cgcattgatt tctaaactga ttccttgtct tgcacgagaa tagatctcta
    11641 ctgagagcgt cgtaaagcca tttcctgtga cgaaaaatgt aaccgcaaaa tcatcaagag
    11701 aataggtaaa tgccataaaa tatccagcta tgattcctgg agaaagataa ggcaaaatga
    11761 tatttttgac tacttggaaa ttattagcac caaggtctct agccgcatcg accattgaat
    11821 cgttcatttc ctgcaatttt ggcaagacca tcaagacgac gattggaatg ctgaaagcaa
    11881 tatgggaaag caatacactc tgaaaaccta atccgaatcc tagtagtgta aagaaaatca
    11941 agaaactagc accgataata acatcaggag agactaataa gatgttattc atgctcaaca
    12001 acgctgtccg tgcacgtcgt tttttcgtgt aatagattcc catagcccca aatgtcccga
    12061 tgatcgtggc gatcaatgca gataagaaag ccagcatgaa cgtattcaat acgataatga
    12121 tcaaacgtgt atcttcaaaa acagcttcat aattttccca agtaaatcct gtaaagctgt
    12181 tcatcgttcc tccttcattg aaggaataaa aaatcagata gaagatcggt gcatataaga
    12241 ataaaaagac gatccctaaa taaagatacg accatttaaa ttttttcatt tatgagcgac
    12301 ctcctttttt cttctcgcct gtcaggagca tcacgatgaa catagcaatg attaaaatca
    12361 caccgattgt agaacccatc ccccagtttt gtgtaaccaa gaaatgctgt tcgatagccg
    12421 ttcctaaagt gatcacccga tttccgccaa tcagacgagt cagcatgaat aaagaaagag
    12481 aaggaataaa tactgcttgg acaccacttt tcacaccatt taatgataac ggaaaaatca
    12541 ctcgtcggaa ggtctcaaag ctattcgcgc ctaaatctct actagcactg atcaaagaag
    12601 gattcatctc ttccaatgca ttgaaaattg gcataatcat aaatggaatc tcgatatacg
    12661 ttgccacgaa cagaaaactg aagtctgtga ataatatctg tttcggcccg atcccgataa
    12721 aatcaaagaa atgattcacg ctgccgtgca cactgaaaat cccaataaat gcgtaagctt
    12781 taagcagcag attcacccat gtaggcaaaa taatcagcat cagccataat tgcttgtgct
    12841 tcagttttgt taaaaaataa gcagtcgggt aactgatgat cagcgtgaaa aacgtgatca
    12901 aaaatgcata ccatacagaa ttgatcgtca tagaaagcga tgtccccgac gtaaaataag
    12961 atagataatt attcagcgtg aattgcccat tcatatcaaa aaaagattga taaatgatca
    13021 ttaatacagg cgcgatcacg aataaaccga gccacaaaat ataagggacg gaatagatcc
    13081 tgcgcattgt tttcattctt gtccccccta ttcttcgtag ctgtcaagac gtgcatcgaa
    13141 ttcttcttca gactcgttga aacgcatgac atggatatct tctggctcaa aggccaatcc
    13201 tactttgctt ccttctttag cttttttcgt tgaatggacc atccattcat tttgctgttc
    13261 atcataacag atgatttcat aatggacacc acgaaacagc tgagtatcga ccgttacgac
    13321 tagttttcct ttatctggtg tcgtgatcgt caaatcctct gggcgtaaaa caacttcaac
    13381 tggttcattt agacgcatcc cgccatcgac acattcaaat tgcttaccaa caaactcaac
    13441 tagattatct tcgatcatta caccgtccac gatattgctc tccccaacaa aatcagcaac
    13501 gaaatgattg attggctcat cataaatatc tacgggtgta ccactttgga cgattttccc
    13561 tttattcatg acaaaaatct catcactcat tgccaacgct tcttcttggt catgagtaac
    13621 aaaaataaac gtgattccta atcgttgttg cagatcccgc agttcatact gcatatctgt
    13681 acgtagtttc aaatcaagcg ccgataacgg ttcatctaat agcaatactt tcggttcatt
    13741 tacaattgct cgtgcaatcg ctacacgctg tctttgacca ccagacattt cactaatctc
    13801 ccgtttttcg tatccaggaa gctggaccat acgcaaagca tcttttacct tcttttcgat
    13861 atctgatttg gacattttct tgatcttcaa tccaaaagca acgttatcaa aaacattcat
    13921 atgaggaaaa agtgcataat cttggaagac tgtatttact tgtcgcttat tagctggcaa
    13981 atcattcaac cgtttccctt caaaatagat gtccccttct gtagcttcgg tgaatccagc
    14041 tataatacgt aggatcgttg ttttaccaca gccggaagga ccaagcagtg tataaaattt
    14101 tccttgttcg atctcaaaac ttactttttt taatactgtt tcatcatcat agcgtttcac
    14161 tacttgatca aatgaaatga ttggtttccc cacgttttta ccccctatat tacaaatatg
    14221 attgcgttgc aacaatcaat acttttgttc gttttttgag atgattcctt aattgatgtt
    14281 gttttgtcgc ttgataatag atcgactgtc ctgcttttgc cacatagacc gtctctccta
    14341 agcttaactc taattcccct tctaatacaa aaataaacgt ttcagataat gaaggttcaa
    14401 acgtcttata ttctcctgca ccttcaaaag tcagcaagac aggctccatt tctttttcat
    14461 tcgaatcagc cacgagccat tccaattcat acccgttttc ttcatcgaaa taaaccgtat
    14521 gatcttcttt tgaataaacc acttgcagat tcgctgcttc ttgatgaaaa aattcttctg
    14581 gagtcacacc taagacttca agaatagaaa agaatgtttc cattgatggt gaacttaaat
    14641 ctcgttccaa ctgggaaata tacccttttg acaaatctgt gcgttcgcct agttcttctt
    14701 gtgtcaaatt cttttggatg cgcaaatttc ttaacttctc accaatttcc ataatttcct
    14761 ccgaatcgaa gttttctaat agtaaacttc ctgtttacta ccactcgtat tagtatacga
    14821 aatttattga aaaaggcaac cctttgtaaa attttttttg ctgaatattt ctttatcttt
    14881 tggaataaat tctagttgtt tgattcttta tttttcacct aatttttgtc taaatttacg
    14941 gaagaacaag aataaaagag taggctttat ggtatgataa aagaaaattt taaaaggagt
    15001 gtctcacgaa tgaacgcttt tcgatctttt caggtaataa aggaacctgt tttccataca
    15061 gcaatcagtg atctgccatt tactgagttg gctgaaaatg aagtcttggt aaaaattgcc
    15121 tattctgatg tcaattataa agatgcattg gcaatgagcg agtctggtca ggtgatacga
    15181 acatatccga tgactccagg gattgattta tccggaactg tcgtccaaag taacaatcca
    15241 cgtttttcaa aagaagatct tgtacttgct actggttttg ggcttggcgt cacccatcca
    15301 ggcggttata gccaatatca aaaagtacca ggagactggt tagttcctct accaaaaaat
    15361 atgacgctgc gtcaagcgat gatccttggt acagcagggt ttactgctat gttgtgtgtg
    15421 aacgctttaa tccgtcaagg tatgacctcc gataaaaaag ttgtcgttac tggtgcttct
    15481 ggcggtgtag gcagtacagc gatagccctg ttacataaac taggattcca gtcaatcatt
    15541 accttttcca gaaaagaaga gtctgtcaca tggctaaaaa gtctaggtgc tttgcaagtc
    15601 gtacgacctg atgaatttct gccagagaca acaaaagctt taggaaaaca gcaaatcgac
    15661 tatgtcatcg acacagtagg tggcgagcag ctaagcagtc ttcttccatt gatctcttat
    15721 aacggtgcag tagctttatg tggcaatgct gggggaatca aactaaacgc gactgtcttg
    15781 ccttttattc tgcgaaacat ccagttgatt ggaattgatt ctgtcaatgt accaatagat
    15841 caacgtcttt ctttatggca acagatggcg gatttgcaga ttgcagatga actggtcgtc
    15901 caagagatta ctctggatca attgcctgaa acagcatcta aactacttgc tggaacacac
    15961 caaggaagaa cactagtaaa tgtaggtgac cataaatgaa gattctaatc acagctggag
    16021 gaacgactga aaaaatcgat caagtacgtg ctattacaaa tcattcgaca ggacgattag
    16081 ggcaagcatt agctgattat ttagctgcca atccggatac cacaatcgat tacgtgacta
    16141 cgaaacaagc actgaaaccg aaaagacact ccaatataac gatttatacg atcgaatctg
    16201 cactggactt atttttacaa ttagaagaac taacgaaaaa agaacactac gatgcgatta
    16261 tccacagtat ggcagtcagt gattttactc ctgcttttag tttttcagaa gaacagctgg
    16321 caaaaaacct acccacttct tcaacgcaag aagaactgga caattggttt gcggaaaatg
    16381 aacagacaga taccactgtt tcaaagatct cttctaacac cgagcatctt gtgctcgttt
    16441 tgaagaaaac accaaaaatc atttcttatc tacgtgaatg gcagccaaaa gcaaaaatca
    16501 ttggtttcaa actattagta gatgtaccaa aggaatctct tctggcagtc gcaaaaaata
    16561 gtctgataaa taacaaaact gattttattt tcgcaaatga tttgacggaa atccatggcg
    16621 agacacacca cggctatctc ttatctaaag atggaacagt ggaagaagct caaagtaaat
    16681 ctgagattgc tgcattgatt actgagaaaa ttagactgga ggaatcaaaa tgaaaaagat
    16741 cttactaggc gttactggaa gtatttctgc ttacaaaagc gctgatctta caagtcaatt
    16801 cgtcaaatta ggtgcccagg tagatgtgat tatgtctaaa agcagcacac attttatcac
    16861 gccattaacc ttgcaatctc tgtcgaagcg cgcagtccat acagacgtca tgcaagaatc
    16921 aaatccgcag gtaatcaatc atatcgaact agcaaaacaa gctgacttgt ttctcatcgc
    16981 gcctgctaca gcaaacatta tcggcaagct agcaaacggc attgcagacg atcttctttc
    17041 aacagtcgca atggcgcttc cagtgggaac accaaaattg attgcccctg ccatgaacac
    17101 gaatatgtat caaaacccga tcactcagcg taatctggaa acattgagat ctgtcggcta
    17161 tcaggaaatt gaaccaagag aatctctttt agcttgtgga gattttggaa aaggtgcctt
    17221 agctgatatt cagacgatca tcgaacaggt catgctgact cttaatgaaa gaaatgctta
    17281 gaaaaaagga gtacttatga aaaatacaaa aaactttaca ttaactgcga tgtttttagc
    17341 tattttgatt ttattagctg ttacgccttt aggttttatc cctatcggtc caatcaatgc
    17401 aacaaccatg catattcctg taatcattgc ttcgatcgtt ttaggaccaa gactaggtgc
    17461 ttttcttggc ggtacatttg gactgatcag catgatccgt agcacagtta tccaaacacc
    17521 tttgtctttt gtcttttctc cgtttatccc agtcatcgga acagaacatg gaagctggaa
    17581 agccttgctg atcgcgttta ttcctcgcat cttgattggg gtggttcctt atttcgttta
    17641 taaaggtctg cgcaaactaa tgaaacaaaa agcagattcc gtttctttat tcttagctgg
    17701 actttccggt tcattgacca atacgatttt agtgatgaat atgatctact ttttattcca
    17761 gcaagattat gccaaagtca tcggaacaaa tttaaatgct gtttattcag ctattctagc
    17821 ggttattttt acaagtggta tacctgaagg aatcgtcgca ggtcttgcga ctgcagcagt
    17881 atgcaatgtc ttgttaagat acttcaagca cccttctctt actaactgat agtcatcgta
    17941 gccgatctct gatttttttc tcttttggtg ttactattga atggaaagat ttgacgtcga
    18001 aaggatgatc caaatgaaaa tttttgttgt aggtgcaaac ggacaaattg gccgacacct
    18061 gataaaagat ttagctccaa gttcccatga aatttttgca ggggtgagag atgttgcttc
    18121 ccaaacatta gtaaaaaaag agaatgtttc gtatgtctct tttgatttaa cttggtccgt
    18181 ggaaaaaatg accgaagcat tcaaaggcat cgatgtattg atttttgctg ccggctctca
    18241 aggaaagaat cttctgcaag tagacttgga tggcgcaatc aaaacagtga tcgcagctga
    18301 aaatgcgcat gtctctcgct atctgatggt cagtgctgtt tatgcagatg aaccagcaaa
    18361 atggcctgag tccatgacag attattatat tactaaacat tatgcagatg aatggctgaa
    18421 acgaactaac ttagattttg tcattcttca accagtcaca ttgactaacg atgaagaagt
    18481 tacttccatt caattaacta agcctaatga gaaggcatca aaaacgatca ctcgctctac
    18541 agttgctgct gtacttgccg ctctagtgga agaaacggat atttctcgta cgactctagt
    18601 actttctgaa ggtagcaaag agttgaatac agcattccaa gaatgggcaa aggaggaata
    18661 gatccatgcg tgccatttca atcaagcatc ctggttctgt agatgtatta gaacaaattg
    18721 aagtgcctgt tcctaaacca aaacaaggcg aattactgat caaagtccat acagcagctg
    18781 tcaatcgaac agatattatg aaaagagaat cagtctcttt gaaacctccc taccctattt
    18841 tgggagtcga agtagctgga gaggtagttg agaataaaag tgatatctcc acttttactc
    18901 ctgggacaaa agtatacgga ttagtcaatc taggtggtta tgccgaatat gctgtgatgc
    18961 ctgccgatcg tgcaatcctt ctccctgata cgctagacta tgtttctgct gctggaatct
    19021 cagaagtttt tttgactgct tatcaaacac tttactggct tggaaaatta caagaaggcg
    19081 aaactgtctt gatccatgca ggagcaagtg gtgtgggaac agcagcgatc caattagcta
    19141 gacagctttc tcacgcgaaa atcatcgtta ctgcaggatc agaagaaaaa ctcgcctttt
    19201 gtaaagaact tggtgcagat gaattgatca actataaaaa acaagacttt caagaagagg
    19261 tacgaaaaat cactgaaggt cggggagtcg atgttatttt ggatttcatc ggcgcttctt
    19321 actgggaaaa gaatttagct tctatagccg ttgatggacg ttgggtcttg attggtatgc
    19381 taggtgggac catcgtgcct gagatagacc ttggtgtatt gattgggaaa cgtgtccaac
    19441 tgcttggtac attattgaca cctagaagtg atcagtataa agcagagctc gcacaaactt
    19501 ttatgcaaaa agctgggcct tttctagaaa atggcaagat tcggccaatc attgaccgta
    19561 cattccaatt cagcgcagtc aaacaagcgc atgaatatat ggaagcaaac aaaaacatcg
    19621 gaaaaatcat tttgaagata actgaataaa atcatgctga tcaaaaaaga ggctgggaca
    19681 gaagcgttta actccaagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt
    19741 gaa
//
LOCUS       DASJPH010000054        19682 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJPH010000054 DASJPH010000000
VERSION     DASJPH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.054"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="U3995_001738"
     CDS             complement(<1..210)
                     /locus_tag="U3995_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797059.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="U3995_001739"
     CDS             complement(216..446)
                     /locus_tag="U3995_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797060.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="U3995_001740"
     CDS             1063..1725
                     /locus_tag="U3995_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797061.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="U3995_001741"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="U3995_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1797062.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="U3995_001742"
     CDS             5145..5459
                     /locus_tag="U3995_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1797063.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="U3995_001743"
     CDS             5472..5846
                     /locus_tag="U3995_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1797064.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="U3995_001744"
     CDS             5856..6200
                     /locus_tag="U3995_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1797065.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="U3995_001745"
     CDS             6271..7623
                     /locus_tag="U3995_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1797066.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="U3995_001746"
     CDS             7683..8825
                     /locus_tag="U3995_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN1797067.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="U3995_001747"
     CDS             8850..9104
                     /locus_tag="U3995_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797068.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="U3995_001748"
     CDS             9360..10544
                     /locus_tag="U3995_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1797069.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="U3995_001749"
     CDS             10541..10678
                     /locus_tag="U3995_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1797070.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="U3995_001750"
     CDS             10683..10904
                     /locus_tag="U3995_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797071.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="U3995_001751"
     CDS             10917..11417
                     /locus_tag="U3995_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1797072.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="U3995_001752"
     CDS             11514..13361
                     /locus_tag="U3995_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN1797073.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="U3995_001753"
     CDS             13364..14260
                     /locus_tag="U3995_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1797074.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="U3995_001754"
     CDS             14310..14699
                     /locus_tag="U3995_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1797075.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="U3995_001755"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="U3995_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="U3995_001756"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="U3995_001756"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1797076.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19682
                     /locus_tag="U3995_001757"
     CDS             18893..>19682
                     /locus_tag="U3995_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1797077.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLR"
BASE COUNT         6649 a         3218 c         4306 g         5509 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg ggtttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 ag
//
LOCUS       DASJPH010000055        19540 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJPH010000055 DASJPH010000000
VERSION     DASJPH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.055"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..240)
                     /locus_tag="U3995_001758"
                     /pseudo
     CDS             complement(19..240)
                     /locus_tag="U3995_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            393..1505
                     /locus_tag="U3995_001759"
     CDS             393..1505
                     /locus_tag="U3995_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1797078.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(1583..1876)
                     /locus_tag="U3995_001760"
     CDS             complement(1583..1876)
                     /locus_tag="U3995_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1797079.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            2044..2358
                     /locus_tag="U3995_001761"
     CDS             2044..2358
                     /locus_tag="U3995_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1797080.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            2379..4358
                     /locus_tag="U3995_001762"
     CDS             2379..4358
                     /locus_tag="U3995_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1797081.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            4371..4697
                     /locus_tag="U3995_001763"
     CDS             4371..4697
                     /locus_tag="U3995_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1797082.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            4713..5231
                     /locus_tag="U3995_001764"
     CDS             4713..5231
                     /locus_tag="U3995_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797083.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            5231..6583
                     /gene="celB"
                     /locus_tag="U3995_001765"
     CDS             5231..6583
                     /gene="celB"
                     /locus_tag="U3995_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1797084.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6667..8172)
                     /locus_tag="U3995_001766"
     CDS             complement(6667..8172)
                     /locus_tag="U3995_001766"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1797085.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(8264..8854)
                     /locus_tag="U3995_001767"
     CDS             complement(8264..8854)
                     /locus_tag="U3995_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1797086.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            9033..10679
                     /locus_tag="U3995_001768"
     CDS             9033..10679
                     /locus_tag="U3995_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1797087.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            10728..11447
                     /locus_tag="U3995_001769"
     CDS             10728..11447
                     /locus_tag="U3995_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1797088.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            11926..12873
                     /locus_tag="U3995_001770"
     CDS             11926..12873
                     /locus_tag="U3995_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1797089.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            12885..13805
                     /locus_tag="U3995_001771"
     CDS             12885..13805
                     /locus_tag="U3995_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1797090.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            13846..15303
                     /locus_tag="U3995_001772"
     CDS             13846..15303
                     /locus_tag="U3995_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1797091.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            15648..16262
                     /locus_tag="U3995_001773"
     CDS             15648..16262
                     /locus_tag="U3995_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1797092.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            16255..17979
                     /locus_tag="U3995_001774"
     CDS             16255..17979
                     /locus_tag="U3995_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1797093.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            17976..19427
                     /locus_tag="U3995_001775"
     CDS             17976..19427
                     /locus_tag="U3995_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1797094.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6270 a         3307 c         3922 g         6041 t
ORIGIN      
        1 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
       61 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
      121 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
      181 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
      241 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
      301 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
      361 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
      421 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
      481 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
      541 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
      601 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
      661 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
      721 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
      781 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
      841 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
      901 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
      961 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     1021 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     1081 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     1141 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     1201 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     1261 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     1321 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     1381 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     1441 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     1501 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     1561 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     1621 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     1681 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     1741 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     1801 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     1861 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     1921 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     1981 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     2041 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     2101 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     2161 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     2221 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     2281 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     2341 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     2401 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     2461 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     2521 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     2581 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     2641 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     2701 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     2761 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     2821 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     2881 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     2941 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     3001 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     3061 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     3121 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     3181 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     3241 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     3301 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     3361 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     3421 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     3481 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     3541 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     3601 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     3661 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     3721 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     3781 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     3841 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     3901 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     3961 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     4021 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     4081 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     4141 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     4201 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     4261 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     4321 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     4381 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     4441 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     4501 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     4561 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     4621 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     4681 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     4741 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     4801 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     4861 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     4921 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     4981 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     5041 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     5101 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     5161 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     5221 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     5281 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     5341 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     5401 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     5461 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     5521 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     5581 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     5641 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     5701 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     5761 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     5821 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     5881 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     5941 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     6001 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     6061 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     6121 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     6181 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     6241 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     6301 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     6361 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     6421 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     6481 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     6541 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     6601 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     6661 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     6721 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     6781 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     6841 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     6901 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     6961 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     7021 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     7081 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     7141 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     7201 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     7261 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     7321 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     7381 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     7441 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     7501 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     7561 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     7621 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     7681 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     7741 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     7801 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     7861 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     7921 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     7981 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     8041 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     8101 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     8161 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
     8221 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
     8281 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
     8341 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
     8401 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
     8461 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
     8521 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
     8581 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
     8641 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
     8701 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
     8761 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
     8821 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
     8881 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
     8941 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
     9001 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
     9061 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
     9121 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
     9181 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
     9241 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
     9301 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
     9361 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
     9421 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
     9481 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
     9541 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
     9601 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
     9661 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
     9721 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
     9781 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
     9841 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
     9901 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
     9961 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    10021 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    10081 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    10141 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    10201 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    10261 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    10321 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    10381 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    10441 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    10501 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    10561 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    10621 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    10681 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    10741 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    10801 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    10861 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    10921 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    10981 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    11041 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    11101 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    11161 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    11221 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    11281 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    11341 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    11401 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    11461 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    11521 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    11581 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    11641 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    11701 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    11761 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    11821 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    11881 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    11941 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    12001 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    12061 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    12121 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    12181 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    12241 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    12301 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    12361 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    12421 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    12481 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    12541 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    12601 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    12661 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    12721 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    12781 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    12841 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    12901 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    12961 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    13021 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    13081 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    13141 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    13201 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    13261 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    13321 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    13381 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    13441 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    13501 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    13561 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    13621 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    13681 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    13741 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    13801 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    13861 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    13921 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    13981 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    14041 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    14101 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    14161 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    14221 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    14281 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    14341 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    14401 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    14461 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    14521 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    14581 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    14641 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    14701 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    14761 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    14821 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    14881 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    14941 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    15001 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    15061 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    15121 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    15181 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    15241 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    15301 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    15361 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    15421 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    15481 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    15541 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    15601 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    15661 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    15721 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    15781 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    15841 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    15901 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    15961 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    16021 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    16081 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    16141 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    16201 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    16261 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    16321 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    16381 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    16441 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    16501 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    16561 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    16621 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    16681 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    16741 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    16801 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    16861 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    16921 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    16981 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    17041 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    17101 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    17161 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    17221 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    17281 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    17341 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    17401 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    17461 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    17521 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    17581 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    17641 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    17701 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    17761 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    17821 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    17881 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    17941 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    18001 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    18061 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    18121 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    18181 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    18241 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    18301 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    18361 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    18421 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    18481 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    18541 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    18601 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    18661 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    18721 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    18781 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    18841 gcgtgctgca aaataaacaa ttagaagtcg ctcaaaaaat gatcactgat ttttttgaac
    18901 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    18961 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    19021 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    19081 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    19141 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    19201 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    19261 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    19321 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    19381 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    19441 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    19501 ttgaggcaag gcagcttttt catgacctct cgataaacgg
//
LOCUS       DASJPH010000056        19480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJPH010000056 DASJPH010000000
VERSION     DASJPH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.056"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U3995_001776"
     CDS             133..3660
                     /locus_tag="U3995_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1797095.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U3995_001777"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U3995_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1797096.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U3995_001778"
     CDS             7448..7789
                     /locus_tag="U3995_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797097.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U3995_001779"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U3995_001779"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1797098.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U3995_001780"
     CDS             9146..11566
                     /locus_tag="U3995_001780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1797099.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U3995_001781"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U3995_001781"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1797100.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U3995_001782"
     CDS             13000..13737
                     /locus_tag="U3995_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1797101.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U3995_001783"
     CDS             13751..14566
                     /locus_tag="U3995_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1797102.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U3995_001784"
     CDS             14579..15226
                     /locus_tag="U3995_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1797103.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U3995_001785"
     CDS             15242..15898
                     /locus_tag="U3995_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1797104.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U3995_001786"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U3995_001786"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1797105.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U3995_001787"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U3995_001787"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1797106.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U3995_001788"
     CDS             18240..18758
                     /locus_tag="U3995_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1797107.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U3995_001789"
     CDS             18865..19365
                     /locus_tag="U3995_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1797108.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DASJPH010000057        19411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJPH010000057 DASJPH010000000
VERSION     DASJPH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.057"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..362
                     /locus_tag="U3995_001790"
     CDS             168..362
                     /locus_tag="U3995_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797109.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="U3995_001791"
     CDS             631..930
                     /locus_tag="U3995_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797110.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="U3995_001792"
     CDS             complement(1027..1461)
                     /locus_tag="U3995_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797111.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="U3995_001793"
     CDS             complement(1693..2403)
                     /locus_tag="U3995_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1797112.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="U3995_001794"
     CDS             complement(2393..3253)
                     /locus_tag="U3995_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1797113.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="U3995_001795"
     CDS             complement(3258..3896)
                     /locus_tag="U3995_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1797114.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="U3995_001796"
     CDS             complement(3911..4210)
                     /locus_tag="U3995_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797115.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="U3995_001797"
     CDS             complement(4253..5737)
                     /locus_tag="U3995_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1797116.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="U3995_001798"
     CDS             complement(5757..6218)
                     /locus_tag="U3995_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797117.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U3995_001799"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U3995_001799"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1797118.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="U3995_001800"
     CDS             7521..8291
                     /locus_tag="U3995_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1797119.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="U3995_001801"
     CDS             complement(8564..9391)
                     /locus_tag="U3995_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797120.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="U3995_001802"
     CDS             9959..11194
                     /locus_tag="U3995_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1797121.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="U3995_001803"
     CDS             complement(12193..12453)
                     /locus_tag="U3995_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1797122.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="U3995_001804"
     CDS             complement(12480..13781)
                     /locus_tag="U3995_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797123.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="U3995_001805"
     CDS             complement(14073..14531)
                     /locus_tag="U3995_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1797124.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="U3995_001806"
     CDS             complement(14544..15875)
                     /locus_tag="U3995_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN1797125.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="U3995_001807"
     CDS             complement(15886..17328)
                     /locus_tag="U3995_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1797126.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="U3995_001808"
     CDS             complement(17315..18079)
                     /locus_tag="U3995_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1797127.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="U3995_001809"
     CDS             complement(18183..19058)
                     /locus_tag="U3995_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1797128.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="U3995_001810"
     tRNA            complement(19313..19397)
                     /locus_tag="U3995_001810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
BASE COUNT         6178 a         3991 c         2985 g         6257 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaagc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata a
//
LOCUS       DASJPH010000058        18556 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJPH010000058 DASJPH010000000
VERSION     DASJPH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.058"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2563)
                     /locus_tag="U3995_001811"
     CDS             complement(<1..2563)
                     /locus_tag="U3995_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797129.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="U3995_001812"
     CDS             complement(2785..3669)
                     /locus_tag="U3995_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1797130.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="U3995_001813"
     CDS             complement(3764..4201)
                     /locus_tag="U3995_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797131.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="U3995_001814"
     CDS             complement(4418..5329)
                     /locus_tag="U3995_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1797132.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="U3995_001815"
     CDS             complement(5706..6344)
                     /locus_tag="U3995_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797133.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..7118)
                     /locus_tag="U3995_001816"
     CDS             complement(6348..7118)
                     /locus_tag="U3995_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1797134.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7536..7952)
                     /locus_tag="U3995_001817"
     CDS             complement(7536..7952)
                     /locus_tag="U3995_001817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797135.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8003..8384)
                     /locus_tag="U3995_001818"
                     /pseudo
     CDS             complement(8003..8384)
                     /locus_tag="U3995_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8510..9175
                     /locus_tag="U3995_001819"
     CDS             8510..9175
                     /locus_tag="U3995_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797136.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="U3995_001820"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="U3995_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN1797137.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="U3995_001821"
     CDS             complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="U3995_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN1797138.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="U3995_001822"
     CDS             complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="U3995_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN1797139.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18057..>18556)
                     /locus_tag="U3995_001823"
     CDS             complement(18057..>18556)
                     /locus_tag="U3995_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1797140.1"
                     /translation="SQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIE
                     QANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEID
                     SAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTE
                     ITIQF"
BASE COUNT         5657 a         3687 c         2738 g         6474 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaagta tatcatactt aaccagtagg cttgataata caaaagaaaa agtatccaca
    11701 aaaaattgtg gatacctcgt ttttatatat caaaatcaaa tgattctgca aaaggattat
    11761 ctataatgaa ttttattcgc tcggtcaaat catttttttg attttttatc actaaataat
    11821 aagtatcttc aactgtatat cgacgattta caaattcaaa aacaaattta gtaaaccgct
    11881 gatttgcatc ttctgttttt gaatccgcaa catagatttc ttcatttgag atacgttcac
    11941 catattcatc ttcaaaataa agccagaaag atgcggggtc ataaacatca ctaattgcat
    12001 ttttttgata gaattctaac gtggttgata atcctgtaat tctgcggttg ctagtcatta
    12061 attcaattgc tactggttcc tttgcattgc tacctttggc taccgtaatt tctagaacag
    12121 ggactagcat ttcttgaata gatgaccctc catgaacgta gctttgtccc ccgccagctt
    12181 tcttaaaaat acttgaagta atcgggacac aaatgtttct agtgtcatca tttcctaaag
    12241 aatctcccaa agagtaattt ttcgtaccaa ttatttcgta ttcttcttca ctaagaataa
    12301 aacgacgttc tacacgatct ttttcatttc tagatggatt ttcaatctta tctacttctt
    12361 caacgtggct tcttttataa ataaatccat gatcagccgt aactataaaa cgatagatac
    12421 tgccatcatt atgcaagcga ttaataagtc gggaaatttc gcttatcgct aaatcacagg
    12481 cctcaaaaac tcgattttca ttcgagtgat cacctgttct atccacttga tcgtgataaa
    12541 tataaatgac tgatttgcca ttaaatactt ctcgtacctc agccttattc atcttaacca
    12601 catcatcata atttaaagct acagcatttg catctcgttg ctggagaatc gcatttcgat
    12661 tggataaacc attcgttttt ttcccatcaa tcagtacttc tgttccttca ttgtaagtta
    12721 gttgattgtt tggtaataag gcggctttac cgaattcagt aactgaaggt aatacagaaa
    12781 gaaagctagc catgtttgtt gaatacttct tttctctaac taacttacgt tctaaattca
    12841 tccctaactc atatcgaaaa gcatctgaaa taataactac agttttaaca gagcgtttca
    12901 ttacagcttg attataaaat tttcgaatgg atggacgatt cttaaattca aaagtttgat
    12961 tccaaatttg tcctgattga tcaagaaact tcgaataatt tgcttcaact aatcgataca
    13021 attggccata tttttgcttt tcttctatat gatcactatt ccaaataaat ttccgataat
    13081 gcgtatcaac ccaatacatt tccttctcat attgagttag caaattatcc atgttcatcg
    13141 gcaagcacaa tctgttttta tccacaagat aataagcatg tttcaataat tgatactcat
    13201 aaatataatc cgatgcgaaa tgcatttttt cgcgctgctt taaactatca aaaattgttt
    13261 gtcctgcaat tttcgttgta gtgtcagaag cctttaatcg ttctgcataa aacattaata
    13321 tttttgcatg aatctcagaa aatacgtctg cttcaattag atcatcaatt tctatactgt
    13381 ctaataatcc atcaccatca atagcctgat atactttttt agatagctgt tcaaagcttt
    13441 ccatgtaacg tgtatcattc ataaactgat ccatgaaagt aataatgttt gctttcaaat
    13501 gtagtatttc aaatcctttg aacttcttcg gcatctcttg atcaatttga taataaaaat
    13561 agttcaaata aagacacatc gtcagctttt ccaatgttgg ctggctatcc gtaaagccaa
    13621 atttcatttg aataaattcc caaaatttag ctgttaactg atacttatca agttccctta
    13681 aaggattctc cctactttct gtaaagcttt ttagaactac acgcagtaaa tcatttaatc
    13741 gaatactttt cgatttcaac aaaacagcca ttatcattaa ttcaatcgtt tctgatttat
    13801 taattgtcat atgcgtacta aattcaccaa aacttctcat gcgatccttt gcattaaaga
    13861 aattacgata ctgtttaact gtctgatgaa atctaagtgg aatagccaat tcattcatga
    13921 taatcgctaa gcggtctgca ctaaattcct ctgaataatt caatacactc aacaaatgat
    13981 tatcttcatc tttatcatcc caacgaggaa atggcgcata gattaaatag ttagtttcgg
    14041 gatcttcata ttccaataaa cgcttagttt taaattgtgc gtccttttct aatcgataga
    14101 tttttgcatt ttgaatgacc aatgagtcaa tttcttcaag aaaatcctga tttgaatcaa
    14161 accaaaaaat gattttccta catccataat taggaaaacg ttgatttaat ttttcttcaa
    14221 tttgttgcgt atttatatct gtcatcaatc tcacctaatc gttctctttc tatacccctt
    14281 atattgacat aatttggata atttcgcacg caatttaaga ataaaaaaga aagttgttag
    14341 aaaacaactt tcttagataa ctattttttt ggttctttga ccccttttgc aaaaatttgg
    14401 aatttctcgc cggttttagg atcacgctgc aacttatcat aattgacaat tacaccatca
    14461 tctaagtcta attcaattcg ttttaatgct aagtgaccca acttctcgtc atactcacga
    14521 acttctttta attgcttctg aatctttaca atttcttttg tcattgcatt tctttctttt
    14581 gggttatcac ttgttgaaat ttgttgctga cgcaattcga ttcgattgtc atacgctttt
    14641 tgtagttcgt gtaggtattc agtacgtact tttcctaatt gatctggtgt atagcgatgc
    14701 aagtacatta acgctttaaa gccattttgt tttcctgagt ccaattgcca ataaattggt
    14761 cgattggagt atgtttttaa atggtctttg taaaaatctt gtacgaagta acgacgaata
    14821 atttctcgac ttgtcccttt gccacctaaa gtgtcagcga taaactggag attttcttca
    14881 agggtttctt taccaaaagt aatctcaacg aaatcaatga aacgattgac aatgtcaatt
    14941 tggctatcct tgaaatactc atcatcggtg atgagtaaaa cattatcagt atcaggttta
    15001 aatttttgat agcgggaagg atcccattcg ccgccagcga agatgagacc atcttcgtct
    15061 agggagtaac gaccgaagat acaacctatt gaataagaaa taaaatcctt aatttccctc
    15121 gccttatcag gtaaccttgc agagatatat tcatcttgaa cctcttttgt taattctttt
    15181 tcaagagaat aatgattaat gaatatgtcg ttcagttcct cttcattcga ttttagagta
    15241 ttaaattgat taaccaactg cctacttaga cagttaaatg cctctgagag atctttttta
    15301 aacatataac ttaacaaaat gtttcttcta aactcgtatg acgtttcaag agcattatag
    15361 tcttttctag ctatatcgat attttcatcc actttttctc ttattttttt taattttctc
    15421 tcagatgtaa ctttaagaaa aggtagtttt cccattggtc cttcatggta atccaaggtg
    15481 ggagaaataa aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct
    15541 tcaaagtcag ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga
    15601 ttctgcaatc taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta
    15661 aaatagtaat taagattttg tggtctagat tttaacttcc cattttcatt aacataattt
    15721 tttatttcat ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt
    15781 ctaaaacgac cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct
    15841 aatgttttag catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat
    15901 ttattgttat ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt
    15961 ccttgagcaa aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg
    16021 atttttttga agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga
    16081 attgcttcta acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt
    16141 atatagcttc catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt
    16201 gcttcttcaa aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt
    16261 aaaaatgagc gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag
    16321 gaagcatatc cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga
    16381 tcttttttga catcagaata gttatcattt acatattttt ttaatttctt atccatttta
    16441 ttaagataag gcggattagt gactaccgct acatatttat caactagcat ttcagccacg
    16501 tcaagaatgt gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc
    16561 gtaaattcgt tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt
    16621 gcctgattat ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat
    16681 tgatcaaaaa ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat
    16741 tcactaatac gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa
    16801 ttcggcgata gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt
    16861 gcaaaataag acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca
    16921 acgattgcac gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat
    16981 acatcgaagg cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt
    17041 tcttctggtt ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt
    17101 aagtatttca acttatttct taattcacta ttgggataat gatctaacca atactttcct
    17161 aaactattgt caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc
    17221 ggaatatctt gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca
    17281 ctattatagt actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag
    17341 aaatcatcct ctggaatact taaaagatca gcgattacac catctttttt aatataagaa
    17401 gctgtaaaca aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca
    17461 ttcaattgat tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct
    17521 gaattatctg ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag
    17581 atatcggcat cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta
    17641 gataatacac gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc
    17701 aatcggttaa accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa
    17761 gaagtgacag aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct
    17821 tttccttgaa ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc
    17881 acatcttcaa tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca
    17941 attaattttc tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt
    18001 tcccttctat atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag
    18061 aattgaatcg tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca
    18121 gctgttaact catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt
    18181 ctcattggta ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt
    18241 gctgcagagt ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt
    18301 ttacgtgctt gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag
    18361 atcaaatact gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct
    18421 tgttcaattt ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc
    18481 ttaaaatctt ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt
    18541 aatatttcct gacttt
//
LOCUS       DASJPH010000059        18504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJPH010000059 DASJPH010000000
VERSION     DASJPH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.059"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U3995_001824"
     CDS             99..746
                     /locus_tag="U3995_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1797141.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U3995_001825"
     CDS             1139..3784
                     /locus_tag="U3995_001825"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1797142.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U3995_001826"
     CDS             4094..5407
                     /locus_tag="U3995_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1797143.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U3995_001827"
     CDS             5397..6050
                     /locus_tag="U3995_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1797144.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="U3995_001828"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="U3995_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN1797145.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="U3995_001829"
     CDS             6947..7147
                     /locus_tag="U3995_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1797146.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="U3995_001830"
     CDS             complement(7401..9080)
                     /locus_tag="U3995_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1797147.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="U3995_001831"
     CDS             complement(9082..9294)
                     /locus_tag="U3995_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1797148.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="U3995_001832"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="U3995_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1797149.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="U3995_001833"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="U3995_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1797150.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="U3995_001834"
     CDS             13269..13466
                     /locus_tag="U3995_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797151.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="U3995_001835"
     CDS             13619..14104
                     /locus_tag="U3995_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1797152.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U3995_001836"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U3995_001836"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1797153.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="U3995_001837"
     CDS             14981..15508
                     /locus_tag="U3995_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797154.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="U3995_001838"
     CDS             15599..16336
                     /locus_tag="U3995_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1797155.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="U3995_001839"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="U3995_001839"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1797156.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="U3995_001840"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="U3995_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1797157.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DASJPH010000060        17516 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJPH010000060 DASJPH010000000
VERSION     DASJPH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.060"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            282..521
                     /locus_tag="U3995_001841"
     CDS             282..521
                     /locus_tag="U3995_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797158.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="U3995_001842"
     CDS             complement(883..1143)
                     /locus_tag="U3995_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797159.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="U3995_001843"
     CDS             complement(1328..1825)
                     /locus_tag="U3995_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN1797160.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U3995_001844"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U3995_001844"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN1797161.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U3995_001845"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U3995_001845"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN1797162.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="U3995_001846"
     CDS             complement(4547..5209)
                     /locus_tag="U3995_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1797163.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="U3995_001847"
     CDS             complement(5219..6034)
                     /locus_tag="U3995_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1797164.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="U3995_001848"
     CDS             complement(6296..6739)
                     /locus_tag="U3995_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN1797165.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="U3995_001849"
     CDS             complement(6873..7211)
                     /locus_tag="U3995_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1797166.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="U3995_001850"
     CDS             complement(7199..7576)
                     /locus_tag="U3995_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1797167.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="U3995_001851"
     CDS             7800..8993
                     /locus_tag="U3995_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1797168.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="U3995_001852"
     CDS             complement(9159..9581)
                     /locus_tag="U3995_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797169.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="U3995_001853"
     CDS             9976..10170
                     /locus_tag="U3995_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797170.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="U3995_001854"
     CDS             10167..10661
                     /locus_tag="U3995_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797171.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="U3995_001855"
     CDS             complement(10823..11995)
                     /locus_tag="U3995_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1797172.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="U3995_001856"
     CDS             complement(12201..13016)
                     /locus_tag="U3995_001856"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1797173.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="U3995_001857"
     CDS             complement(13166..13612)
                     /locus_tag="U3995_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1797174.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="U3995_001858"
     CDS             complement(13609..14613)
                     /locus_tag="U3995_001858"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1797175.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="U3995_001859"
     CDS             complement(14653..14982)
                     /locus_tag="U3995_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1797176.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="U3995_001860"
     CDS             complement(15108..17435)
                     /locus_tag="U3995_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1797177.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5063 a         3478 c         3047 g         5928 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatag
//
LOCUS       DASJPH010000061        17472 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJPH010000061 DASJPH010000000
VERSION     DASJPH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.061"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U3995_001861"
     CDS             complement(105..629)
                     /locus_tag="U3995_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797178.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U3995_001862"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U3995_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1797179.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U3995_001863"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U3995_001863"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1797180.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U3995_001864"
     CDS             complement(2351..3031)
                     /locus_tag="U3995_001864"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1797181.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U3995_001865"
     CDS             3157..4026
                     /locus_tag="U3995_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1797182.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U3995_001866"
     CDS             4052..4567
                     /locus_tag="U3995_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797183.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U3995_001867"
     CDS             complement(4614..5207)
                     /locus_tag="U3995_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797184.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U3995_001868"
     CDS             complement(5232..5411)
                     /locus_tag="U3995_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797185.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U3995_001869"
     CDS             complement(5488..5997)
                     /locus_tag="U3995_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797186.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U3995_001870"
     CDS             complement(6093..6473)
                     /locus_tag="U3995_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1797187.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U3995_001871"
     CDS             6803..7552
                     /locus_tag="U3995_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1797188.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="U3995_001872"
     CDS             complement(7873..9090)
                     /locus_tag="U3995_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1797189.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="U3995_001873"
     CDS             complement(9348..10625)
                     /locus_tag="U3995_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797190.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="U3995_001874"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="U3995_001874"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1797191.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="U3995_001875"
     CDS             complement(11547..12491)
                     /locus_tag="U3995_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1797192.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="U3995_001876"
     CDS             complement(12493..13179)
                     /locus_tag="U3995_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1797193.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13653..13973
                     /locus_tag="U3995_001877"
     CDS             13653..13973
                     /locus_tag="U3995_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1797194.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14127..15032
                     /locus_tag="U3995_001878"
     CDS             14127..15032
                     /locus_tag="U3995_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1797195.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            15029..15802
                     /locus_tag="U3995_001879"
     CDS             15029..15802
                     /locus_tag="U3995_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1797196.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15983..17407)
                     /locus_tag="U3995_001880"
     CDS             complement(15983..17407)
                     /locus_tag="U3995_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1797197.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         5092 a         3603 c         3159 g         5618 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgtta atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg
    13321 taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg tctagttact
    13381 gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc
    13441 ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga
    13501 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg
    13561 taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt
    13621 gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag
    13681 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
    13741 attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa
    13801 tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt
    13861 cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
    13921 tggggatgct actcatcagt atcattggca tcaagattac tagcggccac tagacacttg
    13981 gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa
    14041 aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac
    14101 tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga
    14161 actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg
    14221 ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac
    14281 tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc
    14341 ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa
    14401 caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta
    14461 tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg
    14521 gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt
    14581 tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta
    14641 ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg
    14701 aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa
    14761 tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag
    14821 ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc
    14881 ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata
    14941 tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc
    15001 tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa
    15061 attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg
    15121 aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca
    15181 gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat
    15241 ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg
    15301 agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt
    15361 agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga
    15421 gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta
    15481 tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc
    15541 cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat
    15601 ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa
    15661 tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa
    15721 tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt
    15781 atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag
    15841 ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa
    15901 catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc
    15961 tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg
    16021 caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta
    16081 caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa
    16141 caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac
    16201 ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt
    16261 ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa
    16321 agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg
    16381 tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca
    16441 aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc
    16501 aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa
    16561 ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca
    16621 tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata
    16681 tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag
    16741 aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac
    16801 tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa
    16861 tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta
    16921 atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta
    16981 gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac
    17041 cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt
    17101 tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa
    17161 aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat
    17221 tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag
    17281 ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg
    17341 ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg
    17401 tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt
    17461 tttattttta ct
//
LOCUS       DASJPH010000062        17239 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJPH010000062 DASJPH010000000
VERSION     DASJPH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.062"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..876)
                     /locus_tag="U3995_001881"
                     /pseudo
     CDS             complement(1..876)
                     /locus_tag="U3995_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(894..1568)
                     /locus_tag="U3995_001882"
     CDS             complement(894..1568)
                     /locus_tag="U3995_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1797198.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(1752..2645)
                     /locus_tag="U3995_001883"
     CDS             complement(1752..2645)
                     /locus_tag="U3995_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1797199.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(2719..4416)
                     /locus_tag="U3995_001884"
     CDS             complement(2719..4416)
                     /locus_tag="U3995_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1797200.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(4409..5722)
                     /locus_tag="U3995_001885"
     CDS             complement(4409..5722)
                     /locus_tag="U3995_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1797201.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(5722..9759)
                     /locus_tag="U3995_001886"
     CDS             complement(5722..9759)
                     /locus_tag="U3995_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1797202.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(9772..10230)
                     /locus_tag="U3995_001887"
     CDS             complement(9772..10230)
                     /locus_tag="U3995_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1797203.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(10528..11046)
                     /locus_tag="U3995_001888"
     CDS             complement(10528..11046)
                     /locus_tag="U3995_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1797204.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(11048..11664)
                     /locus_tag="U3995_001889"
                     /pseudo
     CDS             complement(11048..11664)
                     /locus_tag="U3995_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(11661..12524)
                     /locus_tag="U3995_001890"
     CDS             complement(11661..12524)
                     /locus_tag="U3995_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1797205.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(12511..13422)
                     /locus_tag="U3995_001891"
     CDS             complement(12511..13422)
                     /locus_tag="U3995_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1797206.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(13463..14797)
                     /locus_tag="U3995_001892"
     CDS             complement(13463..14797)
                     /locus_tag="U3995_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1797207.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            15005..15835
                     /locus_tag="U3995_001893"
     CDS             15005..15835
                     /locus_tag="U3995_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN1797208.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(16079..16397)
                     /locus_tag="U3995_001894"
                     /pseudo
     CDS             complement(16079..16397)
                     /locus_tag="U3995_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16519..16713)
                     /locus_tag="U3995_001895"
     CDS             complement(16519..16713)
                     /locus_tag="U3995_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797209.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         4914 a         3737 c         2960 g         5628 t
ORIGIN      
        1 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
       61 ggataccgat ccctgagaat ctttaactga aaggctgact ccttgcgctt ccttttctat
      121 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
      181 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
      241 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
      301 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
      361 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
      421 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
      481 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
      541 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
      601 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
      661 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
      721 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
      781 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
      841 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
      901 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
      961 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     1021 tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     1081 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     1141 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     1201 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     1261 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     1321 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     1381 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     1441 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     1501 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     1561 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     1621 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     1681 tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa
     1741 taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa
     1801 caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga
     1861 taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact
     1921 cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt
     1981 gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca
     2041 attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca
     2101 tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg
     2161 gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc
     2221 gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt
     2281 ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg
     2341 acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat
     2401 gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct
     2461 tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg
     2521 ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact
     2581 gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt
     2641 ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct
     2701 gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta
     2761 cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt
     2821 agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc
     2881 tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc
     2941 ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc
     3001 ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg
     3061 tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc
     3121 ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc
     3181 tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac
     3241 tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat
     3301 aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata
     3361 cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc
     3421 tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat
     3481 ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata
     3541 ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc
     3601 gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt
     3661 ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat
     3721 cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc
     3781 gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca
     3841 cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat
     3901 tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga
     3961 aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt
     4021 ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg
     4081 aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt
     4141 atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg
     4201 aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac
     4261 agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc
     4321 ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag
     4381 tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta
     4441 ttaataaaac agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt
     4501 cattcgtttg cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg
     4561 gctctttcgg tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg
     4621 attcataggc agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa
     4681 ctactacata ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga
     4741 ttctcttact tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat
     4801 aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga
     4861 atttgcttac atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt
     4921 tcaagtcatt agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata
     4981 gagcaaaaac agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat
     5041 acttgaagaa gtatggatct cttagataac cgacattttt aggatagata tgaatctctt
     5101 gcagttcctc ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag
     5161 ctgtctgatc gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca
     5221 aataaactgc atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc
     5281 ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt
     5341 cttctgtgat tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt
     5401 ctttaggagt catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa
     5461 tggcaaatgt cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg
     5521 cttcctcaga gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga
     5581 tatctcgaat catccgttta tgcaacacga attccacgac atcttcccct gattcttctg
     5641 ccgacatcgc atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca
     5701 tcaaacacaa cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa
     5761 accataggct gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg
     5821 tccggatcct cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa
     5881 tctgattcga tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc
     5941 tgcttcttcg tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg
     6001 aactacccga tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta
     6061 acctggaagt tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc
     6121 caacaagtct gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc
     6181 gatgatttca tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag
     6241 acgtcggcta ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc
     6301 ttctaaggtt acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt
     6361 ttcgtcttcg atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg
     6421 gaacacttga tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc
     6481 accaatagac agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac
     6541 ggcacgagaa tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg
     6601 attaccagtg tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa
     6661 acctgcacca tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata
     6721 atcaaacgta ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata
     6781 cgtgagatat tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt
     6841 atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt
     6901 ctggctctca atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac
     6961 aaatgaatct ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt
     7021 cccgtcttca tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac
     7081 accagaaagc cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata
     7141 aagttcaaac tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt
     7201 catgtggatt ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc
     7261 tgtgccttct atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata
     7321 ggaattcgga tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac
     7381 aagctcgtag tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg
     7441 atacgtctca tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat
     7501 gtcgctgact gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat
     7561 tccgtccgtg gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg
     7621 gtcatataat gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac
     7681 ttggaaatta cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc
     7741 agtagtgata tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt
     7801 gcaaaaattt ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat
     7861 ttccccttca cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata
     7921 cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat
     7981 ttctggctct tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc
     8041 tgccgatctg ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc
     8101 ttgctggatc gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt
     8161 cttgttgttc gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt
     8221 tttcaataca tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat
     8281 tcctaaacca atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg
     8341 atgataagca actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt
     8401 attgttatta gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac
     8461 ctctccataa cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata
     8521 ggtaactgga tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct
     8581 gaacaaaacg tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc
     8641 tccattagtg ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc
     8701 atctcccaca acgattagga ttttttctgg atttctattt tctccgccat tgtctttata
     8761 caatgtttca tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg
     8821 atttaatgct gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat
     8881 gtccgaactg atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat
     8941 accgattcga atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc
     9001 ctcaatcaac gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact
     9061 cgctgactta tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc
     9121 gatgacatct agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc
     9181 tgtgctgctt ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa
     9241 gtaactgttt tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt
     9301 tctatcttct tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt
     9361 cgctcccccg tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc
     9421 tttgtctcct tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc
     9481 attcgttgta aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat
     9541 atattcatag gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa
     9601 ctggcgttga atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc
     9661 catctcattt tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc
     9721 ttgtaattct tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc
     9781 gttcacatcg gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga
     9841 ataaactttt ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc
     9901 atcttcatca taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc
     9961 ggctgccgca tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa
    10021 agccagatgc cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata
    10081 gtcctcatct aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag
    10141 ggtcgtcata tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa
    10201 ctgcaaaagc ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc
    10261 ttctttcaca acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat
    10321 tttaaatcaa atgatatcct ttttagttaa aaaacaccaa ttatacaaca tttattatta
    10381 aaattaaatt ataaaatttt ataacgttct catttaacaa aacttaagtt attaagacaa
    10441 atgaataaac aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg
    10501 cactcatgct ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac
    10561 acttcctaaa atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct
    10621 cgaataagcg atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc
    10681 gaacaacgca gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt
    10741 cttcggttta cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct
    10801 tgtcgttttt ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat
    10861 ggctagacca tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca
    10921 aataaaacca ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc
    10981 cgttttccgt tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg
    11041 caacatgtta ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga
    11101 cttgaaaata aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc
    11161 agaatgcgac aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat
    11221 taaaaaacaa gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga
    11281 ttttgtaact aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg
    11341 tatagccaat tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa
    11401 aataaacact tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt
    11461 tccagaatag tgaaccgagc tttttctctt catttttttg taatgataaa tcatatacag
    11521 aactgttcga cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag
    11581 ctgtaaatgg ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt
    11641 tttaccaatc gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg
    11701 aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct
    11761 gccatcagat aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt
    11821 aacgtgtatt tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct
    11881 gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa
    11941 tgactccaaa tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta
    12001 ttgatcgata agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat
    12061 ggaacgatca ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga
    12121 gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc
    12181 tttttcccag gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca
    12241 ttaccgatca ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga
    12301 aaggcggcaa tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga
    12361 ggataacgaa cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga
    12421 atgatcaaaa tgattcctat tactgtcatg aggacataac ttagtatttt tttgtttgcc
    12481 gattcacact ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac
    12541 ccacttaggt gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat
    12601 cattgacata gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg
    12661 cagattataa aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata
    12721 agcttcttga aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat
    12781 aggagtgatt aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat
    12841 ttctgctgat tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat
    12901 tgctcctcca gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc
    12961 aaacagccaa gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc
    13021 agatggagaa agcagctgca tccataagat gtagactgct actcctgata agactgccgg
    13081 taaatagaaa gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc
    13141 taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt
    13201 gtataacgat ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc
    13261 aatgaaattc atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag
    13321 cataggccct agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc
    13381 ccatcttgct tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg
    13441 tacctagaca gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt
    13501 gtgcatctgc caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt
    13561 cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt
    13621 taaagttttc gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat
    13681 tcactaaatt ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt
    13741 tttcttgtac ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt
    13801 ttgctccgta tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt
    13861 cttctttttc tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca
    13921 aactagagta ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat
    13981 cagatacacc agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat
    14041 cttgccagtc ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt
    14101 tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc
    14161 ctaaattcca taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta
    14221 gtttacgtgc tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc
    14281 cagcttcttt aaatatcttt ttattataaa aaatgacttg tgtatctgta ttgaatggga
    14341 tcgcataagg atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag
    14401 aagtgtcttt ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg
    14461 caacggaatt tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata
    14521 aagatttagt ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt
    14581 gtgattgatt gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc
    14641 aaaaggacca gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg
    14701 cacttgaact ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg
    14761 aacttgccag caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt
    14821 aaccgatgcc ggttttaata taacgatatc attttgttct gaaaataaca atttataagg
    14881 tatgattcta aaaaaattat taatgatacc aatactttat ataattcgcg agcgctttct
    14941 ttgaattttt taatctttcg tagcagaatg agaatgtatt aaaaatagat taggaggaat
    15001 tttgatgttt agacaccaga aagaattgca gtttgaagca aaaccagatc gtccgaatcc
    15061 attaatcgcg aaatatctgc aggagctgat cggcggccaa tatggcgaga tgagtgtagc
    15121 tatgcagtac ctgttccaag gctggagctg ccgaggagaa gaaaaatata aagatatgct
    15181 gatggacatt gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg
    15241 tttattagag ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag atccaacaat
    15301 cggtgccatt atctctggaa tgaatccaca gcacgcgatc gtagctggct taggacctcg
    15361 tcctgctgac agcgtgggca atccatggag cggtgcctat atcattgcga gtggcaactt
    15421 gttagcagac tttagagcta atttaaatgc cgaatcacaa ggaagacttc aagtagcacg
    15481 tatttacgaa atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag
    15541 agatagctat catcaaaatc tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg
    15601 ttcaatctta gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga
    15661 cttctataac ttctcagagg gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc
    15721 actggatggc gaaggcgaat atcaatggtt gaaagaacct aagatcgaag gcgctgcacc
    15781 aaaattagaa ccagtaacac caaaaatgta tgggacacct ccattcaaga aataaatatc
    15841 tacccgtaga aacacccgtt ttttgttcca cacgctttgt gagcaaaact gactaaagtc
    15901 attaatcata accacccttc aaaagggtgg ttttctctag agctataagc tcaaatcacc
    15961 ggccagcgcc taaagacgct ggctttcata ttttatgttc aagccatatt gcctttgcca
    16021 gttttgccca cctcttaaaa ggtttatgta ctactcctta gcccttaaaa ggatcgtctt
    16081 actctcgtac actcagctta tctaacgcac tatcatgttt ttcttgttct tgaatgtact
    16141 ttttgatggt cgcttcattt tgtcctactt ttgtcacata gtatccttcc acccaaaagt
    16201 gtcgataccc aaatttatat tttagatttg cacaatctct tgatgatctc ctgaatattg
    16261 gctcgatatt gattataaat tatttttctt ctatactttg gtgtgaatac aatgtggtat
    16321 ttacacatct actttgtacg ggctaaacta tttcccttta ttgctccacc cgcatagcaa
    16381 gtggttttgt atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa
    16441 aacccgctgc ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat
    16501 ctcttacggc aatctatttt atttttttga ttttttataa atggaaaatg cttcaagaag
    16561 tccaagaaat aaagccgtat aaaaacaaga acctatgaaa tctttattta ttagatattc
    16621 aacagaaatc ccgataacac tagccaacaa tatatttatc agaagtgtaa aataaggatg
    16681 ctttttatag aatagaacta gtgttgtttt catagactct cctttcttca tatttccaat
    16741 taatcacaaa ttcacggcat tactagaaaa attaaagggg cgcagaagtg gaaaaatact
    16801 cttcgacttt ccctacaaac caggccatct tgtgtttaat aactatacca aatctaaggt
    16861 acctctaaat ctaaattcca tttgtggcgg tgacctccaa tttttaactc ttctatctcc
    16921 catccagtat acataacctt gatacttcga ccacttatat atcgaacaca caaataccta
    16981 tccattgcag gagtactacc taacttagtt gctacttgca tctaaataac agctgaaaag
    17041 ataggactta ctcttagtaa agtatctgaa ctattaattt ttaaactatc atttttatcc
    17101 tcaactacat gcacatctgt tgcaacttca ctttctgtct catccagata aatgattaag
    17161 gaatcgggaa ttgcaatatc ttcagctgac gctggaatag ttgagctaaa ttagtcatca
    17221 tatctttatt ataaaataa
//
LOCUS       DASJPH010000063        16446 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJPH010000063 DASJPH010000000
VERSION     DASJPH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.063"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="U3995_001896"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="U3995_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1797210.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(632..1618)
                     /locus_tag="U3995_001897"
     CDS             complement(632..1618)
                     /locus_tag="U3995_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1797211.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1641..2207)
                     /locus_tag="U3995_001898"
     CDS             complement(1641..2207)
                     /locus_tag="U3995_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1797212.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2221..2637)
                     /locus_tag="U3995_001899"
     CDS             complement(2221..2637)
                     /locus_tag="U3995_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1797213.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3094..5028)
                     /locus_tag="U3995_001900"
     CDS             complement(3094..5028)
                     /locus_tag="U3995_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1797214.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5309..6265
                     /locus_tag="U3995_001901"
     CDS             5309..6265
                     /locus_tag="U3995_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1797215.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6355..7458
                     /locus_tag="U3995_001902"
     CDS             6355..7458
                     /locus_tag="U3995_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1797216.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7471..8424
                     /gene="menA"
                     /locus_tag="U3995_001903"
     CDS             7471..8424
                     /gene="menA"
                     /locus_tag="U3995_001903"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1797217.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9238..10584)
                     /gene="gor"
                     /locus_tag="U3995_001904"
     CDS             complement(9238..10584)
                     /gene="gor"
                     /locus_tag="U3995_001904"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1797218.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10718..11971)
                     /locus_tag="U3995_001905"
     CDS             complement(10718..11971)
                     /locus_tag="U3995_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1797219.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11977..12900)
                     /locus_tag="U3995_001906"
     CDS             complement(11977..12900)
                     /locus_tag="U3995_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1797220.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13121..15613)
                     /locus_tag="U3995_001907"
     CDS             complement(13121..15613)
                     /locus_tag="U3995_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1797221.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15620..16087)
                     /locus_tag="U3995_001908"
     CDS             complement(15620..16087)
                     /locus_tag="U3995_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1797222.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16223..16296)
                     /locus_tag="U3995_001909"
     tRNA            complement(16223..16296)
                     /locus_tag="U3995_001909"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16260..16262),aa:Arg,seq:acg)
     gene            complement(16354..16427)
                     /locus_tag="U3995_001910"
     tRNA            complement(16354..16427)
                     /locus_tag="U3995_001910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16391..16393),aa:Pro,seq:tgg)
BASE COUNT         4851 a         3447 c         2926 g         5222 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      721 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      781 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      841 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      901 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      961 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
     1021 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
     1081 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1141 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1201 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1261 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1321 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1381 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1441 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1501 tgattgcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1561 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1621 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1681 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1741 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1801 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1861 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1921 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1981 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     2041 cgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     2101 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2161 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2221 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2281 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2341 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2401 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2461 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2521 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2581 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2641 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2701 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2761 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2821 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2881 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2941 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     3001 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     3061 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3121 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3181 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3241 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3301 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3361 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3421 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3481 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3541 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3601 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3661 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3721 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3781 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3841 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3901 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3961 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     4021 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     4081 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4141 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4201 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4261 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4321 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4381 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4441 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4501 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4561 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4621 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4681 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4741 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4801 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4861 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4921 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4981 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     5041 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     5101 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5161 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5221 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5281 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5341 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5401 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5461 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5521 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5581 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5641 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5701 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5761 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5821 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5881 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5941 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     6001 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     6061 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6121 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6181 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6241 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6301 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6361 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6421 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6481 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6541 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6601 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6661 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6721 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6781 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6841 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6901 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6961 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     7021 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     7081 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7141 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7201 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7261 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7321 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7381 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7441 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7501 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7561 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7621 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7681 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7741 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7801 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7861 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7921 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7981 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     8041 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     8101 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8161 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8221 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8281 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8341 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8401 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8461 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8521 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8581 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8641 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8701 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8761 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8821 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8881 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8941 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     9001 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     9061 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9121 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9181 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9241 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9301 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9361 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9421 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9481 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9541 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9601 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9661 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9721 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9781 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9841 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9901 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9961 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
    10021 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
    10081 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10141 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10201 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10261 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10321 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10381 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10441 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10501 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10561 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10621 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10681 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10741 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10801 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10861 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10921 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10981 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    11041 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    11101 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11161 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11221 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11281 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11341 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11401 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11461 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11521 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11581 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11641 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11701 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11761 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11821 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11881 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11941 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    12001 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    12061 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12121 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12181 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12241 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12301 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12361 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12421 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12481 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12541 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12601 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12661 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12721 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12781 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12841 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12901 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12961 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    13021 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    13081 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13141 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13201 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13261 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13321 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13381 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13441 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13501 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13561 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13621 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13681 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13741 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13801 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13861 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13921 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13981 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    14041 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    14101 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14161 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14221 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14281 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14341 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14401 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14461 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14521 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14581 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14641 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14701 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14761 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14821 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14881 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14941 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    15001 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    15061 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15121 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15181 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15241 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15301 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15361 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15421 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15481 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15541 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15601 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15661 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15721 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15781 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15841 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15901 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15961 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    16021 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    16081 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16141 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16201 ggaatatact attccgtatc gacgcgccca agaggagtcg aacccctaac cttttgatcc
    16261 gtagtcaaac actctatcca gttgagctat gggcgcatat tcaatttaaa aaaattataa
    16321 atcatttgaa aacaaaaagc aagataaaaa aaatcgggaa gacaggattc gaacctgcga
    16381 ccccttggtc ccaaaccaag tgctctacca agctgagcta cttcccgttt aaaaaataaa
    16441 aaagac
//
LOCUS       DASJPH010000064        15741 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJPH010000064 DASJPH010000000
VERSION     DASJPH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.064"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="U3995_001911"
     CDS             complement(24..722)
                     /locus_tag="U3995_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1797223.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="U3995_001912"
     CDS             complement(715..2067)
                     /locus_tag="U3995_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1797224.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="U3995_001913"
     CDS             complement(2290..3690)
                     /locus_tag="U3995_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1797225.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="U3995_001914"
     CDS             3851..4588
                     /locus_tag="U3995_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1797226.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="U3995_001915"
     CDS             4713..5192
                     /locus_tag="U3995_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1797227.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="U3995_001916"
     CDS             5189..5956
                     /locus_tag="U3995_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797228.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="U3995_001917"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="U3995_001917"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1797229.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="U3995_001918"
     CDS             complement(6859..7461)
                     /locus_tag="U3995_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1797230.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="U3995_001919"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="U3995_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="U3995_001920"
     CDS             8142..8726
                     /locus_tag="U3995_001920"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1797231.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="U3995_001921"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="U3995_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1797232.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="U3995_001922"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="U3995_001922"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1797233.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="U3995_001923"
     CDS             10627..10806
                     /locus_tag="U3995_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797234.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="U3995_001924"
     CDS             10828..11853
                     /locus_tag="U3995_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1797235.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="U3995_001925"
     CDS             12028..13272
                     /locus_tag="U3995_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1797236.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="U3995_001926"
     CDS             13425..14372
                     /locus_tag="U3995_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1797237.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="U3995_001927"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="U3995_001927"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1797238.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="U3995_001928"
     CDS             complement(15274..15516)
                     /locus_tag="U3995_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797239.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="U3995_001929"
     tRNA            15637..15709
                     /locus_tag="U3995_001929"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4374 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt t
//
LOCUS       DASJPH010000065        15381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJPH010000065 DASJPH010000000
VERSION     DASJPH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.065"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..857
                     /locus_tag="U3995_001930"
     CDS             54..857
                     /locus_tag="U3995_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1797240.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1442..1897
                     /locus_tag="U3995_001931"
     CDS             1442..1897
                     /locus_tag="U3995_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797241.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2016..3074
                     /locus_tag="U3995_001932"
     CDS             2016..3074
                     /locus_tag="U3995_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN1797242.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3116..5065
                     /locus_tag="U3995_001933"
     CDS             3116..5065
                     /locus_tag="U3995_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1797243.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5068..8709
                     /locus_tag="U3995_001934"
     CDS             5068..8709
                     /locus_tag="U3995_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797244.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9109..9447
                     /locus_tag="U3995_001935"
     CDS             9109..9447
                     /locus_tag="U3995_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797245.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            9557..9778
                     /locus_tag="U3995_001936"
     CDS             9557..9778
                     /locus_tag="U3995_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797246.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            9798..11954
                     /locus_tag="U3995_001937"
     CDS             9798..11954
                     /locus_tag="U3995_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797247.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            11966..12199
                     /locus_tag="U3995_001938"
     CDS             11966..12199
                     /locus_tag="U3995_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1797248.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12189..13055
                     /locus_tag="U3995_001939"
     CDS             12189..13055
                     /locus_tag="U3995_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797249.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13070..15040
                     /locus_tag="U3995_001940"
     CDS             13070..15040
                     /locus_tag="U3995_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797250.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15207..>15381
                     /locus_tag="U3995_001941"
     CDS             15207..>15381
                     /locus_tag="U3995_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797251.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         5709 a         2175 c         3101 g         4396 t
ORIGIN      
        1 gtaccagatt aacatgatta aaggagcgca aaaagaagat tggagagtaa tcaatgtctc
       61 aagaagttac cgaaaagatt attgatagtg atgtagattt agtatcagaa caaacaaatg
      121 caattgtgcg ttataacaat ggtctgaata caacagcttt gcgaggattt aagcctgttg
      181 aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca gcagaagtaa
      241 cttttgattt caacctcttt cgttatttgg tcaaatacga cagaagagaa aaggacagtt
      301 tttattctgt gttaaaagat ttgagtgaca aactaggtaa tttaacctac cgttttgaag
      361 attcagacgt ctatgaacag ctgtggcttt ttcaaaaatt ccggatagat aaacagaagg
      421 aaacagttac aattaaggca aatgaggaat ttgaatttat cttaaactca attggcaaaa
      481 actttactcg ttttgaactt gataatttta caaaactaag aagtagctac acaaaagaat
      541 tgtatcgtca tctcatgcaa tacagaaata aagagtggcg aaatggttat tgggtagtca
      601 aagttgaaga ttttagaaga gcattaagta taccttcaag ttatagaatg acaaatattg
      661 ataaaagaat actagagcag gcgaaagaag aatttttagc tcctgatgaa aacggaatac
      721 aggtatttga aacttttgat tatgaaaaaa tctatgcaaa gaaaggaaga agagttgatc
      781 ggctcagatt cacttttagt gagccagaat caaacttgcc aaccatttca atgcataatt
      841 ggttagaaga agattgagaa gaagcaggtt gtaattttaa tacaacctgc ttctttaaaa
      901 aagtatgggt aacacttttg gacaataaat gcaagaagtt tttttgtaag caagtttcag
      961 tctaaataat atttgaaaga atggctaatc tatacaaatt ttaacaacta tgggtaacac
     1021 ttttggacaa gtcaagaaaa aaaacaattt ttcttaattt gttgatcgac taaaggtaac
     1081 acttttggac aacacagaat aaaaagtccg gtgatatcag ttttggtagg aaacatctta
     1141 atatctttgt gagattgaag gtaacatttt tggacaagtc aactatttgt taaaagttaa
     1201 tatagagtaa ttagataagt tttttctttt cgtctgataa atggataata ttagttattt
     1261 tattgctaat tagtctttca tgatgactga aatagctaaa ataaatatct cgttggataa
     1321 ttgctgatac tttaagggat atctaaattt gctgattgat ttatttatgt tattatgata
     1381 ttataataac ataaatgtgt tacgcccgaa cctcatttta aaaaaatgaa ggggtgagac
     1441 aatgaatgct attaattggc atgaatttgt taactatatg gagagcttac tagatgatct
     1501 aaaaagtttt agtattgctg atgcagtaaa gcatgggtta ccagttagta caacgatgaa
     1561 attacgagga ttgaaaattc cagatgaaat tattaagctg agaactaaaa caattattga
     1621 aaccgcaaaa gtcgttgaca atatgaacca tgaactaaaa ggcaagaaaa caacttacga
     1681 gaaagatttt gaacgttttt taaaatctgt tattggaaaa tatctaggta aaaaatcttt
     1741 cagtgaattg agagatgaag agattgtacc tactccaatg gttagcaaaa tccgaaaaaa
     1801 caaagccgag gggaattact atcttggtac aatagcaaaa atcattgatt atttggaaaa
     1861 taaagagcaa aagaaaggaa cgaagaaagg aagataattt ttgtgttaaa tattaatggg
     1921 gaagaatata ccgaaatgat tcaaaatccc gaacctagaa ttaccgaaaa aacgtctgat
     1981 tgtttgctct tttatgaaag ggggcagaca aactaatgga aaaaaacgtc aattcaacat
     2041 ctaccaaaga tcaatacagt attattggag ggttgcttgg tgataaggat aagagaacac
     2101 gtcacggatt acgcattatg cttgcaactt ttctaggagt gcttttttta ttaacaggct
     2161 tgggtagcta catttatcaa ttaggaaact ataattatta tctcgtgaac aatacaacac
     2221 ctataggctc ttcattggag tttgctcgat caggagcaga gatcactatt tcagatgtgt
     2281 ggacagatca aaacagagat gtaacagtag taaaactcgg ttactcgcaa aacgctagaa
     2341 agttgttatc tacgcaagga aaaaactaca acttatatat ggcaacgaaa gaaaaagatc
     2401 gtcctaaaaa gcttgaaatg agctatggga tgttaagtac tgatggggat gctttcttat
     2461 tcctaaaagg taaattagat gaaagagctt atcaagtttt tattgcaaac caagtatcac
     2521 taatttctgg tgatgataca gctgacgatg ataccaaagt caatgtgaat gaagcagatt
     2581 cagttacaaa agcactttct caatattcta tgaatgatgt taataatgaa ggagtaattt
     2641 cttttgggaa aaaagcaaaa cctgttaata ctcctgataa tatcaatttc agaataaatc
     2701 catattcaga ttcaactaat gtctatcaag gaagctttct taccaaagat ggcgatattg
     2761 attattcaaa agttgtagca gttacaagta agaataatgt tgtaagtaag ttagaagatc
     2821 agttaaatgc ttcaaatact aacatcaaga aaattgaagc tacaaaagct gagtatctgg
     2881 aacgtttaca acaaaatcct gacgataaag attcgcaaga aagtattcgt caactagaca
     2941 gtcgaatgga gaccgaaatt aaagcaatca atgatacaca aaaattgatt gatcaataca
     3001 aaaatgctga ctttaacaaa gattcttttg gagacatgaa gacgacattt aagtacctaa
     3061 ctctgactga ctagaagtaa attattctta ttttagagga agtgaacaca aacagatgga
     3121 aaatagtacc gagtttttta agaatattca aactgaattt aatcatgctc tcacaccaat
     3181 gaaaccaccg aaacataagt tagtgggacg tgatgacgaa ctccatgagt tgtcactgat
     3241 cttgaataga ccggaaacac cgcaagctat cttgttaggt gaagccggaa caggtaaaac
     3301 agcacttgca agtgaatggg ctgaaagaca gcgacaagat ggaaagaaag tacaagtata
     3361 caaattatca atcggacaat tgcgtgttgg gggagatgat gtcctccaaa cacgtttaga
     3421 aacattaatt ccaagattga aagaatatga gcgaatttta aaatctgagg acaaagaaaa
     3481 tgaatttgtc ctcttcattg acgagtttca taaagttgtt agctcttttg gtagtggcaa
     3541 taaaattggg ggtgattcgc taaaagaacc gttagcaaat gaatcacttc aattgattac
     3601 cgctactaca agaaaagaat atgaaaacta tatcaaaaga gatgatgcct tagggagacg
     3661 gttacaaccg ttagaattga atgaactaaa aagcgatgtt gtcgtcgata ttttgagaca
     3721 atggctaaat acatcctctg ttaaagatga tcctcaggct tcttttaaat tatcgcaaag
     3781 gattcctgat gaattacttc aagaaatcgt acaagcaaac aagctttatc gtgaggattt
     3841 gtccgaaccg gctaaatcaa ttgccgtaat ggaggatttg attgcaaaag atcgagtaga
     3901 cggaaatgct tataatcgag aaagtctatt gcaaatattt ggtaagtata atattcagct
     3961 agattctgat tttgatatgg aaaaacttca agaggaatta aagtccataa aagggcaacc
     4021 gttagcagtt tatactataa caatgttgtt taaacgtatg aagcttggat ttgatcgttt
     4081 taaaggtcga aagcaaccaa aagcaactgc tctgtttaca ggatcaacag gggtcggtaa
     4141 aactgcaatg gctaaagccg tggcaagagg actttatggc gatgaaaata aagttctctt
     4201 agttaatatg ccggactaca aaacaaaaga tgatgcaatg cgttttagac gactcatagg
     4261 tactcatgta aggcatgatc cttctgcgat tattgtgtgt gatgaatatg agaaagctgg
     4321 tgtagaaaca ctcgatgcct ttttatcaat tttagatgaa ggtaggataa gttttactga
     4381 aagaggatgg gatgatcgag aagttcacga cactgtttca ttgtcaaata caattttatt
     4441 tgcgacaaca aatgcaggtg cggagatgtt cgattcgtta aacagatata gctcactatc
     4501 tcgtgttaca aacaaggaaa attctaatga atatagcact gaactaaaaa gagaataccg
     4561 aaaagtcgca tcagggatca aacaagcctt gattggtgaa ggtctaaagc ctgaagttat
     4621 taaccgattt ggacgaatag ttccattttg ggctttatcc gaacaaactc ttctggaaat
     4681 tgcttataat gaaatgttga agagtgctga attagtacga aaacatttaa atatagattt
     4741 acgtcttcca cctaggaaag aaagagtata tggtcaaaat ttttatgcag acgatctctc
     4801 catgcactta gtttttgaca tggcttcaca agaggattct aaatcagggg gtgcaaggaa
     4861 tatcttgcat ttagttgaat ctgagtttga agaagcgatt actgaagcaa taattactta
     4921 tccaaacgca aaaatattcg atgttaagtt gagtgaagcc ggagcgtttg aaggaaaagg
     4981 tgattctaat gctgaaatta ttgcaaagcc ttccgttgct gaaagtaaag aaaacaagat
     5041 tgtattttcg attggagggg cgtagctatg aaattcaaga aatatttact acaaaacctt
     5101 agaataaaac gatgggtcgt aagaggtcta gttattggag gagtattagc agttacttta
     5161 ggcgctcctt ttggaatgaa tgatccaact cctgacaatc aagatagcaa acaagaaaca
     5221 gttataaata agaacgttgc atatgcggaa agtaaaccgg cagaacaagg aatgtggaca
     5281 ctatatgctc gtatcctaat gggagataaa gaagttaatg atagtgccgc taaaactgga
     5341 caaatgaatt tgttaggaca aggtggtatc agtgccgatt tcccttattc tgacatttct
     5401 aaacaaggta attcgattgc agggggtaat gcaggtactc aactagcatc tactttagca
     5461 acttatagtt actacggata tattgaaaca gtctcaggaa atgctattgc cagtcaagca
     5521 tcatccattc taggaagtat tggtaggacg attggtagtt ttatcggatc aattagttta
     5581 atcctttcta atattgctga atccataaat gaactccttg gaaaattttt catgagtatg
     5641 aatatatttt ctcttatggg gtggggagaa ggtggatcgg atgtatctaa tccggtatct
     5701 aaagcattaa ctagcttgct taacaatttg ggattaaata atgaattatt taaagcgata
     5761 gcaagcttat ctatgctctt tttcacagca atactaggtt atcaaggaat gaaattattg
     5821 tcgaaagatg gtgttaactc gaagggatta tcaccagtac ttgaaaaatg gattgttcgt
     5881 atttttgtat tgtttatatt cttaccagtg gcaggatcgc tattgggagt ttatatcaac
     5941 ggcatccagt cagcaaaaaa agatggagat ataccaacaa atgttgtttc tgactacatc
     6001 attaacacaa gactttgggc ttctactcaa aatcttgctc catcatctga tactggtggt
     6061 gatgtacctg acggaaagcc tacatccgaa atcaagttcg ttgatacaga ttatgaacca
     6121 tctaaaaaat ctgcaatgat aagagcgatc aatactagca gttatgccag acaaaataat
     6181 gatctagatt ctaattcagt cggtatggct ctattaaaag aatggtcatc gaatgatact
     6241 ttcaatatta atacttatgc cggtgatgtt aatgtcgccg aaaatgatgc ttatatggga
     6301 agagcgcctg ctgtttggac aggctataat ttaggggatg gaaaagaagg aagtgcaatt
     6361 aagaaaccaa ccaatttaaa tggatatatt tggtcagcaa atcaaaatgt gacagacgac
     6421 aatcgcaagc ctgatggaaa aggaacagcg cataactttg atgctactca accttggggc
     6481 gttgctaaca atgcaacttt tagcacacag tcggtagctt tattgctcca atcggcactt
     6541 gatacatcgg gagctaagtt ttatgcgtat aatatcgctc cttcaggaat tcaaggcagt
     6601 gctaagaata tgtcaacagt aaaaacagaa tggcgtacag catccttagt tggtcatgga
     6661 gctatgggta agtttggctc gtggctagcg atgaacgctg aatcaatcgt tttagcagtt
     6721 ctttacttag catgtgcttg ggcgatgttc aatgtgaata tcgtgaaagc atttaaacgt
     6781 gaatttattc ggatttggga agtagttcta acaggatcac ttgcatcagg agcaggtgtg
     6841 atggcaattt cattaggtgt tgttacaaca tctctgatcg gactacaatt acctatcttt
     6901 ctgattactt ttattcgtgc atttggtgat actgctgtgg gattcattcc taatacatta
     6961 gttggcgatg gcgtagcaaa tattgttcga tctgctatga ttttaggtgt aggatatctt
     7021 ttagtcttta aaaaaggcga aaaagtaaat aatttgatta atatgcttat tggcttacca
     7081 cttgaagtag cgtttgggtt tgatgaacgt gctaagttat tatttggtaa tagaaatgat
     7141 gctgtgcgag ttgtggacgg cgtttctaaa gcaatcaaga aaagccaagg agctaaaaat
     7201 ccctattatg atggtggcaa cagctcagta aaagatatgg ttgcaggagg tcttggagca
     7261 gttggagcgc aaaaaatggc tgaacgtttg aacaatatgg gagatagcaa tgctgatgct
     7321 aagtccattg ctgataaagt aattcctgaa acaccagaag gaatgtcatt caacggtggc
     7381 ggagttggtt ccacaatcaa taatacctct ttcaaagatt tagttcgtca agatcaacta
     7441 cgtaataact cccaacaata tattgatcat ttaggacgta tggttgatga aaacggaaac
     7501 tttgtcgatg aaaatggtga aatattaaaa gatagtaaat acggtaaaga tgctaaaggc
     7561 aactttattg acgaaaaagg gcgtttggtc gatgaagatg gaaaccttat agatactaat
     7621 ggtaaaccat tggaagatag tgaagatggt aatattggta tggatagcga aggacgttta
     7681 attgatgaaa aaggtcgctt agtcgatgaa gatggaaatc ttgttgacga aaatggacgc
     7741 aggttgctaa atggtgattt tggtcatgat agtaacggac gtttagttga cgaaaaaggt
     7801 cgtttagttg atgaagatgg aaatcttgtt gatgagtttg gtaataaact tggtaaacac
     7861 agcgatggat ataatgtgaa tggagattta gtcgatgagg atgggaatat cattgagacg
     7921 agagatgcgc ttgtggcaga tcgaaataga gaactcgaac aacaacggca tacgccaatt
     7981 gctgttgcaa ttcctgagaa tgaagaagac gagattactt tagatcaaac tcatcaagaa
     8041 ctgaggaatg aaatgagtgg tgaggacgtg tcagacagag tgcatcggga attaggagat
     8101 ggagcatctg gtgaaagtca gtcaagagag cttaacagca actttgataa tagtgctatt
     8161 cggaatgaca cagtacttaa agacagagaa tcgctaacaa aagaaacgga taaagaacaa
     8221 tcagatgtga agaaaaagtt cattcctact cctaatgtta atcaaacaga taaatatgaa
     8281 acaaatattg gatcaggaat gtcgaaagac aatcaatcaa ttagagaacc tgaactgaca
     8341 aataaaaata atagaacaaa taattctaaa tctacttcag ataattcagg gaaatctaat
     8401 caatcaacca agaaaacatc aacagctaaa tcagccgttg aatatctatc aaaaactggt
     8461 aaaaatgttt acacctcagc agataaagct ttagcaaaag ctccaaaagg tagtctagca
     8521 gggataacct atgaagctaa aaacgaagct attaaaatag gtaaaggaac ggctaaggtt
     8581 gccaaggcta ctgctacagg agcatccaaa actggtaaag tagtgaagaa atctaccatg
     8641 tcagtcaagg actatattgt aaaatacggt aatcaaaaat caactgaaaa taagtctgaa
     8701 gacgagtaaa aagaaggagc attgatttgc tccttctttt tactttgatt tctaactgaa
     8761 gatgtcaagg aaaatttcta tttatgcaaa aaaattattt taaattgtct ataaatgaat
     8821 taaatattta ctgttcaaag ttgtataatt aaaaagggct tatgctggta aagaacagaa
     8881 ttattatcta aacaaaaact tgataaacca gcatttttag ccaataaaaa aagtgaaata
     8941 atttaatgga aaatctcttt taatcatcga aaatttatgt tattatgata acataaagat
     9001 gtaaatatcg agacgtcttt ttcttagtta gatcattgac aagtgaacaa ataagatttg
     9061 tcgatgatat aattaactta ttaaaaatat taaaaaggag gtttttatat gttcgattct
     9121 attaaaaatt tggttagcgg aatgacgcct gatatgaaaa ctttagtaca agttgttttg
     9181 tatttcttgg cattcgtttt ttcgttgaaa ccaggattca ctgcaatgaa attttttggt
     9241 gatgcaaaat ggggagatgg attaaaatat gttggggcga ttattgcaat ctttattatt
     9301 cctgtaatta caaccgttat gctttacaca atgggtacta aaacaggtaa tgatttgaac
     9361 caaaaagcta atatcatatc agcacttatt ccagttgttg gtatctatct agtttcaaaa
     9421 tttccaaaaa atgaactgtt aggttaaaag tgaattgaac aaatttggat aatatgccga
     9481 tttgttcaat ttacttttta ccaaaaaact acgctccaag cgtagtttag aagtgaggga
     9541 agaaagtaaa tgtaagatgg tttttgatag agaaaaaatg ttagctcacg ccaacgaagt
     9601 attaatgagc agtctgaaag gtaccgaact ggcaaagata atgaatatga atgttaacca
     9661 gttctatgat tatcgaaatg ggagtaaaaa gattgaaaaa gccagacttg aaacgcttat
     9721 taaattcgaa aaagcatatg tatatatgct agataaacag aaaagaacta ttgactgaaa
     9781 aaaggagttt tacaataatg gcaaaattca aagatattac cgaatctatt aattatgtgg
     9841 cagaaacagt tcctgtagtt gcactctctt tgaagccaca attactcgaa gtgtacaaaa
     9901 aagaagaaaa agaagcgaag gcaaaaaaag ctaaagcaaa ggtatggaaa gaattcgatg
     9961 cgttgctgac caaagattta ccaaagaaaa attatatggt aattgaagat gtacaaaagc
    10021 tcaacagtga gggagttata gatgaggatg atgctattct cgattctgag aagtatgtcg
    10081 ttctattgaa gtcaagtttg aattatgtag ctgatctacc agaaaaattt aaagagaagc
    10141 tattagcagg tgtccgtttt atggatagta gtggtcaatt attagttatt ccggatgatc
    10201 ctaatgtgtt tgaatcagaa gttgaagaag ctacagactt ctatagcgca gtagagcaat
    10261 tagaagtagc tactaatgga ttagaacgaa ttgctgagac cttcaactct tctgaagaag
    10321 ttgaatttga agccgtagca gaaaatgtac cagatgaaaa agaagctctt tttgtggatg
    10381 ataacaaacc aaccgaagca gaaaaccaag aagtaagcat agaagaaaca caaatacaaa
    10441 cagaaaccta ccaacaacca aaaattgatg aaaaacccga atcccaaagt gacaaagaaa
    10501 attatgttta tgaagctgaa aaaatcacat ctgaaagtga tgtaccgaaa aacttagatg
    10561 ctgatttttt agaaaaaaat tttgaacaga tcattgctga caaatttcaa cctgtagttt
    10621 tagatgagat taatgaccct gtcgatttaa acgaggagat gctatccata cctgagttga
    10681 aaccattgct gtttttagct aaagagagta tgaacgctcg catctctatt gctaataata
    10741 aaatcaacag tgaattaaat aagacgaagg agcaattgat cctcaaagga aagcaaatgt
    10801 tagctgatga gcttttgcgg atagaggaag caacctcatt aaatggtcaa aatcagttta
    10861 caaatacact atcggttgct aaagagagct acgaagcaga agcatctaaa attgatgatt
    10921 tagtgatgga aaaatccaat atcattcgta aagattatga tatggcgaaa caggcttatt
    10981 tggatgctga actaaagaaa ctagagcaac ggtatgatgc tgagcattta ccagaagtta
    11041 aacaacaaga agatgaatat agaaaagcaa ctgaagaaag tcttgatcaa cagtaccaag
    11101 aagcaattga atctattaaa aacgatgcat tgaaacaaag ccaagaagca aaagaagttg
    11161 tggtggaacg aattatcaat tcattgaaac cagaaattga aaaatcatta acagacgtgc
    11221 aagaaaaaac tttagagatt gtcgaaaaaa caaagtcaga aaatgaagct gagttccatg
    11281 atacaaaaga gaaaacaaca gatgtggttg tcaaatattt tgcgaacaaa gaagaagttg
    11341 aaaagcaggt agctaacaac acgaaagaat tagaaacaaa acttgtagag caacaaaaaa
    11401 ctatcgaaga atataaagat cgaatgaaag aggtcaaaga tttagcagag ggttataaat
    11461 taaatagcga aaagttggca gaacaaagtg aagctttaag agaagactta tctcgttccc
    11521 gaagtgatct agctcaaatg gcagaaaaaa cgaaatcatt agcagttgct acagaaaatc
    11581 aatcaagttc aaaacgtgat tttacaagta aaaagatggc tactatcatt tctgtcgctg
    11641 caatggcttg cggaacgatc ttgggatcgt cctactttat tagtcataat aatcaacaag
    11701 cacaggctca aactcaaaca tctatttcga gagtggaaaa ttcgattggg acaaatacga
    11761 actctgcttc aacaaatagt agctatgaag caacaccaac tacagagttc aaagttggga
    11821 aagatgttgt catcagtatt gatgggaaaa caactaaggc tgaagtgatt tctattgatg
    11881 gagaaaaagc agttgtgaaa actccagacg gaaatcagta caaaattcaa gctcacttat
    11941 ctgataataa ctaaggaggg ctattatgcg agaagaaaaa atagtttatc cagtaattca
    12001 aaacaatcaa gaaaacatga cctcttctca gcacttacag gaagcacagc tcgtgtatca
    12061 ggtaaatgaa ctaccacaaa cgaacgagac aatgagcaag tcaatatctt taattggctt
    12121 gcttattgtt gcaatagtga taactgtagg tgtttaccgt aagttgaacg tgaaaggaag
    12181 tatttctgat ggcagataac tcaaactatg gatttaaaca taacttacat caaaaacctt
    12241 ctgaaacaat tgtcagagtt gaatggcttg aaaagcatgt caatagcacc tattctgtta
    12301 cagcaaaaag attaattgta tcagctatag ttgttgcagt cgttgttctt ggatcaattg
    12361 tttacttagt ttctgatacg attaaactga aatttatttt tgttccaacg ttattaatga
    12421 taattggatt tcttattcta attagccagt atatgtttat tgatccatct agtggttcat
    12481 tgatttctgg ttctattaga ctgttcaaat cagtagttaa ttcagcaaga gtatctatgg
    12541 ggaaaaagaa accatttcga ggcacagaga ttttaaaagt gaaacaagat ggaacaatcg
    12601 tatatcgaga tgggagtgta gcattaatgt tcaatctaga tggggctaca tcactaaccg
    12661 cttttccaga agaagttatg gctcaggaat cgaaatcaat acaatatcaa aataccagag
    12721 atcgttcctt agctgaaatc aaaatcactt catctcaacc tcagaatacg gaaagacaaa
    12781 ctcgaaatat gaaaatacaa aaacgaaatg caaggagtaa caaagcagtc tttgacataa
    12841 tagatcagca agaacgattt gtatcagaat atattgatgg tgtgaaaaca acagtaatcc
    12901 aatatcttat cttgaaatct caatctaaga tggtattgga tgaaggtgtg gaacgtttag
    12961 aaaactatac gagtagagga ctttattata gtgtcactca gttgtcagct aaacaaacaa
    13021 aagaaatatt atctgatatt tatgcactga aatagaggtg agagattaaa tggaaattat
    13081 gggtagatca caactattaa atttcaaaaa aaagagaaaa actaaaaaag aacaaaaaac
    13141 tagaaattca aaggtagtga ataatacctt tgttagtgat ttaacatctt tagttggtta
    13201 tcaatttaca ccatcttaca ttaggacagg taagcggtgg ggaacaattg tgaaattagt
    13261 gaatacttat ggaatgaata gaaattatgg atttggttgg atggttaaag ctattcctga
    13321 agtgagagta gaaggggtaa aagcctatct aattgaagca gataagccag tgtcacaaaa
    13381 agatcaacaa gaaataatgc acgagaatgt tgggggatca acgaatagtt atgaaaataa
    13441 tttagaagga aaaaattcag atgctgaaat tagcaaaact gttatgcgga ttatggattt
    13501 gaatacagca tctttactag acagcatggc aaataaaatt attgattgga agctagaaat
    13561 cctattagta tccgattctc ctaataaaat cagcgaacag ctaattaaac tccaacggtt
    13621 gtatgatgaa aaaatgtatg gattaaagct tatgtcatat ggaggagatc aacgtgatct
    13681 gtttgataga cttttaagtg aaccaatcgg gaatgtctat gagtatacgt ctatgagttc
    13741 ggattttgca ggaaatgatc atgcaattcg caaaggttta gatgatgcga atggatgggc
    13801 tatcggcact atgtctacta gctacgcaag tggtgtagct tttatggcac tggatgattc
    13861 ttttacaagt aaagttttag tggcatcaca caaaaacgct acaattaaag gctatgatga
    13921 aaaaatctca gcatcatcac tttgggggca attgattgct aataatgcaa tggcaaatca
    13981 taaacgaact tttcatattg tcttaaataa atttgattac tttggtgatc caactggtag
    14041 aacatttgtt gccaaacctt cgttaaacag tgtaatagaa aaggttaatt tggctgaaga
    14101 aggtcttaac tctttggagc tttttggtaa tcaaaaggac attggtgatt tatatagacg
    14161 gaatctcgat aaactaactc agatttttta tttgttagca ggcagaacat tagattctgc
    14221 aactagagtt ttattgaaaa aagaattagg taacttttat ataagtcaga aactttggga
    14281 tattaattat gaagaacgtt caaaactttt ggtgaacagt agtgatacaa tgccagttct
    14341 tggggatttt attagctatt tgactaatgc tgaaacaaaa gccaaaaaat ctggtactga
    14401 aaaagaagtc gatcgtatta ttgtattaaa agacacattg agaacagcat tagacagctt
    14461 ctctaggatg tttaatcaaa gaacgacact gaaagaaatc ggtaaaagtg acaaattaca
    14521 gttttactat aacttaggtg atctaagagc agaaaccaat gttatggaag ctcaattttt
    14581 aaataccttt gatttcatca catcacaagc aaaaaaagat gatattgtta tgattcacgg
    14641 cttcgataag ttatctattg aaaccgttgg gctaattaaa aaaagattaa caatgcttga
    14701 agaagatgga attcgtcttg cctttctatt tgatacaatc ggttcaggag aagacaatga
    14761 atacaaagaa gatattgaaa cagctgatat attcaataca gatggaaaaa tttaccaaag
    14821 ttttgaaagt gattttaatt acactatcat tggaaagatg accagtggag agttaatgaa
    14881 gtatgaaaaa aaagttgctc ctgctagaaa aggagaagat gatcaaaaac tttctgttga
    14941 tttgagacag tatttgacaa caggaagaag taataaattt cagatcagga gaccagtgga
    15001 tctgactaat aattttgtta ttggagagtt tattctttaa gttttatctt tgattttgag
    15061 tgtataagga aaggataaga aaaaacatgt atatttgaga ctaaaaacag tcttagagaa
    15121 catggtacgt tttgtgaaaa gagaactata gaaatttcat aaagtgaaac cttactgagt
    15181 gaaataatta tagtggaggt taaataatga aaccaaacat aaaaatttta gatcgaatat
    15241 ttctaggtag agacacagaa gtaatcctaa ttcaacatga agaaggattt gaagttagta
    15301 taggtattca gaaacttcaa aaacctcatt attgtaatca gttgtataaa aactttactg
    15361 atgaagaaaa agcgagagtt t
//
LOCUS       DASJPH010000066        15255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJPH010000066 DASJPH010000000
VERSION     DASJPH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.066"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U3995_001942"
     CDS             248..1021
                     /locus_tag="U3995_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1797252.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U3995_001943"
     CDS             1018..1785
                     /locus_tag="U3995_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1797253.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U3995_001944"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U3995_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1797254.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U3995_001945"
     CDS             3750..4070
                     /locus_tag="U3995_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1797255.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U3995_001946"
     CDS             4070..4420
                     /locus_tag="U3995_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1797256.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U3995_001947"
     CDS             4587..5525
                     /locus_tag="U3995_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1797257.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U3995_001948"
     CDS             5540..6370
                     /locus_tag="U3995_001948"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1797258.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U3995_001949"
     CDS             6414..7439
                     /locus_tag="U3995_001949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1797259.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U3995_001950"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U3995_001950"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1797260.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U3995_001951"
     CDS             8517..8957
                     /locus_tag="U3995_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1797261.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U3995_001952"
     CDS             8960..9562
                     /locus_tag="U3995_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1797262.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="U3995_001953"
     CDS             complement(9625..10728)
                     /locus_tag="U3995_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1797263.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="U3995_001954"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="U3995_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1797264.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="U3995_001955"
     CDS             12294..12689
                     /locus_tag="U3995_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797265.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="U3995_001956"
     CDS             12689..12940
                     /locus_tag="U3995_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797266.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="U3995_001957"
     CDS             12983..13378
                     /locus_tag="U3995_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797267.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="U3995_001958"
     CDS             13375..14631
                     /locus_tag="U3995_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN1797268.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="U3995_001959"
     CDS             14643..14966
                     /locus_tag="U3995_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797269.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="U3995_001960"
     CDS             14959..15225
                     /locus_tag="U3995_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797270.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DASJPH010000067        15231 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJPH010000067 DASJPH010000000
VERSION     DASJPH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.067"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="U3995_001961"
     CDS             278..787
                     /locus_tag="U3995_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1797271.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U3995_001962"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U3995_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1797272.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U3995_001963"
     CDS             2322..3302
                     /locus_tag="U3995_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1797273.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U3995_001964"
     CDS             3424..4842
                     /locus_tag="U3995_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN1797274.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U3995_001965"
     CDS             4913..6382
                     /locus_tag="U3995_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1797275.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U3995_001966"
     CDS             6392..6859
                     /locus_tag="U3995_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1797276.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U3995_001967"
     CDS             6895..7767
                     /locus_tag="U3995_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1797277.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="U3995_001968"
     CDS             7849..10383
                     /locus_tag="U3995_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797278.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="U3995_001969"
     CDS             10539..11312
                     /locus_tag="U3995_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797279.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="U3995_001970"
     CDS             11305..12096
                     /locus_tag="U3995_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797280.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="U3995_001971"
     CDS             12106..12597
                     /locus_tag="U3995_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797281.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="U3995_001972"
     CDS             12615..13025
                     /locus_tag="U3995_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797282.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="U3995_001973"
     CDS             13029..14429
                     /locus_tag="U3995_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN1797283.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="U3995_001974"
     CDS             14452..14826
                     /locus_tag="U3995_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1797284.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2137 c         3045 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta g
//
LOCUS       DASJPH010000068        15069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJPH010000068 DASJPH010000000
VERSION     DASJPH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.068"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="U3995_001975"
                     /pseudo
     CDS             78..686
                     /locus_tag="U3995_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="U3995_001976"
     CDS             706..1734
                     /locus_tag="U3995_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1797285.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="U3995_001977"
     CDS             1731..2489
                     /locus_tag="U3995_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1797286.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="U3995_001978"
     CDS             complement(2575..3315)
                     /locus_tag="U3995_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1797287.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="U3995_001979"
     CDS             3376..4629
                     /locus_tag="U3995_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1797288.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="U3995_001980"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="U3995_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="U3995_001981"
     CDS             5300..5626
                     /locus_tag="U3995_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1797289.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="U3995_001982"
     CDS             5694..6407
                     /locus_tag="U3995_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1797290.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="U3995_001983"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="U3995_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="U3995_001984"
     CDS             7690..9555
                     /locus_tag="U3995_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1797291.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="U3995_001985"
     CDS             9603..11012
                     /locus_tag="U3995_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1797292.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="U3995_001986"
     CDS             11120..11821
                     /locus_tag="U3995_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797293.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="U3995_001987"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="U3995_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13307..13882
                     /locus_tag="U3995_001988"
     CDS             13307..13882
                     /locus_tag="U3995_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1797294.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="U3995_001989"
     CDS             13882..14322
                     /locus_tag="U3995_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1797295.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="U3995_001990"
     CDS             14338..15039
                     /locus_tag="U3995_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1797296.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5082 a         2501 c         3179 g         4307 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaa
//
LOCUS       DASJPH010000069        14983 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJPH010000069 DASJPH010000000
VERSION     DASJPH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.069"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..1015)
                     /locus_tag="U3995_001991"
     CDS             complement(218..1015)
                     /locus_tag="U3995_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1797297.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="U3995_001992"
     CDS             complement(1012..2196)
                     /locus_tag="U3995_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1797298.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="U3995_001993"
     CDS             complement(2221..3468)
                     /locus_tag="U3995_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1797299.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="U3995_001994"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="U3995_001994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1797300.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="U3995_001995"
     CDS             5448..5762
                     /locus_tag="U3995_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1797301.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="U3995_001996"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="U3995_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1797302.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="U3995_001997"
     CDS             6447..7091
                     /locus_tag="U3995_001997"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1797303.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="U3995_001998"
     CDS             7106..7435
                     /locus_tag="U3995_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1797304.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="U3995_001999"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="U3995_001999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1797305.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="U3995_002000"
     CDS             8423..9247
                     /locus_tag="U3995_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1797306.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="U3995_002001"
     CDS             9240..9587
                     /locus_tag="U3995_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1797307.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="U3995_002002"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="U3995_002002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1797308.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="U3995_002003"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="U3995_002003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1797309.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U3995_002004"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U3995_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1797310.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U3995_002005"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U3995_002005"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1797311.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         4637 a         2834 c         3142 g         4370 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taa
//
LOCUS       DASJPH010000070        14942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJPH010000070 DASJPH010000000
VERSION     DASJPH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.070"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(510..1076)
                     /locus_tag="U3995_002006"
     CDS             complement(510..1076)
                     /locus_tag="U3995_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1797312.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="U3995_002007"
     CDS             complement(1076..2032)
                     /locus_tag="U3995_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1797313.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="U3995_002008"
     CDS             complement(2032..2688)
                     /locus_tag="U3995_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1797314.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="U3995_002009"
     CDS             3058..5076
                     /locus_tag="U3995_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1797315.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /locus_tag="U3995_002010"
     CDS             5503..7917
                     /locus_tag="U3995_002010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1797316.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="U3995_002011"
     CDS             8265..9818
                     /locus_tag="U3995_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1797317.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="U3995_002012"
     CDS             complement(10199..11983)
                     /locus_tag="U3995_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797318.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="U3995_002013"
     CDS             complement(11998..13719)
                     /locus_tag="U3995_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797319.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="U3995_002014"
     CDS             13819..14580
                     /locus_tag="U3995_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1797320.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4860 a         2745 c         2956 g         4381 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 ac
//
LOCUS       DASJPH010000071        14549 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJPH010000071 DASJPH010000000
VERSION     DASJPH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.071"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..816
                     /locus_tag="U3995_002015"
     CDS             4..816
                     /locus_tag="U3995_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1797321.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="U3995_002016"
     CDS             813..1613
                     /locus_tag="U3995_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1797322.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="U3995_002017"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="U3995_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1797323.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="U3995_002018"
     CDS             3110..4819
                     /locus_tag="U3995_002018"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1797324.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="U3995_002019"
     CDS             5031..9389
                     /locus_tag="U3995_002019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1797325.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="U3995_002020"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="U3995_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1797326.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="U3995_002021"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="U3995_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1797327.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="U3995_002022"
     CDS             11227..11520
                     /locus_tag="U3995_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1797328.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="U3995_002023"
     CDS             11517..11828
                     /locus_tag="U3995_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1797329.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="U3995_002024"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="U3995_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1797330.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="U3995_002025"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="U3995_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1797331.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DASJPH010000072        14026 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJPH010000072 DASJPH010000000
VERSION     DASJPH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.072"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U3995_002026"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U3995_002026"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1797332.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U3995_002027"
     CDS             complement(2260..2511)
                     /locus_tag="U3995_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1797333.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U3995_002028"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U3995_002028"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1797334.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U3995_002029"
     CDS             3461..3679
                     /locus_tag="U3995_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797335.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="U3995_002030"
     CDS             complement(3968..4480)
                     /locus_tag="U3995_002030"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1797336.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U3995_002031"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U3995_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1797337.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="U3995_002032"
     CDS             complement(6933..7376)
                     /locus_tag="U3995_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1797338.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="U3995_002033"
     CDS             complement(7377..8165)
                     /locus_tag="U3995_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1797339.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="U3995_002034"
     CDS             complement(8162..9103)
                     /locus_tag="U3995_002034"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1797340.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="U3995_002035"
     CDS             complement(9280..9867)
                     /locus_tag="U3995_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1797341.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U3995_002036"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U3995_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1797342.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="U3995_002037"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="U3995_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1797343.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="U3995_002038"
     CDS             complement(12784..13719)
                     /locus_tag="U3995_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1797344.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..>14026
                     /locus_tag="U3995_002039"
     CDS             13905..>14026
                     /locus_tag="U3995_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797345.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3899 a         3132 c         2470 g         4525 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgt
//
LOCUS       DASJPH010000073        14020 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJPH010000073 DASJPH010000000
VERSION     DASJPH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.073"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..636)
                     /locus_tag="U3995_002040"
     CDS             complement(<1..636)
                     /locus_tag="U3995_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1797346.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVM"
     gene            complement(648..2063)
                     /locus_tag="U3995_002041"
     CDS             complement(648..2063)
                     /locus_tag="U3995_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1797347.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(2056..2889)
                     /locus_tag="U3995_002042"
     CDS             complement(2056..2889)
                     /locus_tag="U3995_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN1797348.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(3048..3278)
                     /locus_tag="U3995_002043"
     CDS             complement(3048..3278)
                     /locus_tag="U3995_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797349.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(4025..4492)
                     /locus_tag="U3995_002044"
     CDS             complement(4025..4492)
                     /locus_tag="U3995_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN1797350.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(4567..5007)
                     /locus_tag="U3995_002045"
     CDS             complement(4567..5007)
                     /locus_tag="U3995_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1797351.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(5037..5330)
                     /locus_tag="U3995_002046"
     CDS             complement(5037..5330)
                     /locus_tag="U3995_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797352.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(5361..5555)
                     /locus_tag="U3995_002047"
     CDS             complement(5361..5555)
                     /locus_tag="U3995_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797353.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(5661..6113)
                     /locus_tag="U3995_002048"
     CDS             complement(5661..6113)
                     /locus_tag="U3995_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797354.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(6139..6600)
                     /locus_tag="U3995_002049"
     CDS             complement(6139..6600)
                     /locus_tag="U3995_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1797355.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(6801..7652)
                     /locus_tag="U3995_002050"
     CDS             complement(6801..7652)
                     /locus_tag="U3995_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1797356.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(7667..8467)
                     /locus_tag="U3995_002051"
     CDS             complement(7667..8467)
                     /locus_tag="U3995_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1797357.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(8510..9400)
                     /locus_tag="U3995_002052"
     CDS             complement(8510..9400)
                     /locus_tag="U3995_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN1797358.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(9402..10343)
                     /locus_tag="U3995_002053"
     CDS             complement(9402..10343)
                     /locus_tag="U3995_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN1797359.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(10576..10911)
                     /locus_tag="U3995_002054"
     CDS             complement(10576..10911)
                     /locus_tag="U3995_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797360.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(11067..11240)
                     /locus_tag="U3995_002055"
     CDS             complement(11067..11240)
                     /locus_tag="U3995_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797361.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            complement(11237..11425)
                     /locus_tag="U3995_002056"
     CDS             complement(11237..11425)
                     /locus_tag="U3995_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797362.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            11503..11727
                     /locus_tag="U3995_002057"
     CDS             11503..11727
                     /locus_tag="U3995_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1797363.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(11724..11879)
                     /locus_tag="U3995_002058"
     CDS             complement(11724..11879)
                     /locus_tag="U3995_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797364.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(12019..12231)
                     /locus_tag="U3995_002059"
     CDS             complement(12019..12231)
                     /locus_tag="U3995_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797365.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(12234..12446)
                     /locus_tag="U3995_002060"
     CDS             complement(12234..12446)
                     /locus_tag="U3995_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797366.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            12721..13131
                     /locus_tag="U3995_002061"
     CDS             12721..13131
                     /locus_tag="U3995_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797367.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            13144..13596
                     /locus_tag="U3995_002062"
     CDS             13144..13596
                     /locus_tag="U3995_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1797368.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            13687..>14020
                     /locus_tag="U3995_002063"
     CDS             13687..>14020
                     /locus_tag="U3995_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN1797369.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEI"
BASE COUNT         4018 a         2881 c         2163 g         4958 t
ORIGIN      
        1 cataactttc atttcttgaa aaaatttttt ctggtaagag tcatactttt ctttgatata
       61 gattccggca tttccatatc tttctgctct ccaaggttca atattacttg ctcttaactt
      121 ccaaccatca tcttcttcca caggctctag caatcggaaa gctactccac aagctccttg
      181 aaaagttgca gtttcagaat caagcattgc gaatctcatg gaatcaagat tgcttgttaa
      241 tttgttaaaa gcttctggaa ctgtcggaag ttctatatca ttttttaaga atttatcttt
      301 taatctttga atgagtgttc ttttttgttc cgatacatcg taatcccatt tgataggtat
      361 accagtaaaa tgattgactg cttggtctac gacaattgaa tacataccag catgaagttt
      421 attattaact tttacaatat cagtgttagg ctttggtcta ttatctattt cattcttttc
      481 gctagtataa gctagatatt ttctttcgcg atcagcaaaa aatggtttca tatcttctat
      541 aaaaccattc ggatcaaaca ttccatcttc aatttgtgtt gcatatttaa cacgtagctt
      601 cttatatctc tctaaagata atactgtact aatcaaaagc tcacctccta aaattgaata
      661 aattgatagt gacttggtgg ctcataaaac gctaaagcca gtgcgtcagc aatatcggga
      721 ctaccaatat ttcgcttttt catatcatct ttactttcta aacgtatacg acttctactt
      781 gtcattttga atttgcgagt acttaattct ttgattaacg aactatcaga aggcaattca
      841 ataacaggct gttctccgtt aagatttgct gtcatatttt cttctaacat ttccttgatg
      901 tttccccata gttgagtacc taaattatcg taaaaatcgt cttctgatgt tgaaccgtta
      961 ttcactccaa acacctcaaa aggataatgt ttgtcttcta taagttcttc taggcggtcg
     1021 gttacaccac ctccgacacc agtgtcatcg actttaatca tcactttatc aatactcgga
     1081 tattgactca ttagattctt ggccatgttg atgacatatc ctgttgtttc catggtgcta
     1141 cgttttgaat acttctcata ctccaatgcc ctggtagcaa ttctaggaaa gagaatcgta
     1201 gaatcatcac catatcgagc tacgtcgaca ccaatatgag caacctttgt tttattgact
     1261 aaagaatcac taatttgttt ttctgtagcc aattcaactg tttcaagact gataaatgaa
     1321 tccaacgcac ctttcggaaa ttctccaaaa atacggacac gagcaacatc actttctttt
     1381 ccatattttt taagaatcat ttctatattg tctttgtttg tacgtttact atcatagctt
     1441 gatactttat gaactctgta tttatcacgg tctgaattat gggaatcata aaagacacct
     1501 tcaatattat tggggtttcc acacatcaac agcttattat caaaacctga tagcgtacca
     1561 agaatagctt ccataattgg atcagacaca ccagaagctt catctaccac aatcaacata
     1621 tggtcctcgt gaaaaccttg catactttct ggtttagtcg ctgttctagc cgtagcaaac
     1681 caacgctctg aatcaccaac catataaatt ttggtctttg tccacttcag taagtttttg
     1741 atcaagctat catttaacca tttagctacc tctgcccaaa gtacatcgta aagttgcttc
     1801 attgtcggag ctgttgctat tactttagcg tagggtcgac acgttaagaa ccaaagtatt
     1861 gctcctgctt ctaatgctgt ttttccaact ccttgaccag aacgaacaga aacctttgaa
     1921 aattcagcta aatcatttaa aacattttct tgccattcat caggattcaa atgcaaaata
     1981 tcttggcaaa aagctactgg tttatcatag taataatcaa tggcagaacc aatatcagca
     2041 aaaggaataa attcattatt cattttcttt caccgcccgt ttgtttgcgg catttaaaac
     2101 tgcctgtttc cattcttcta tttcttcacc tgaattattt ccgccaattt catttgtctc
     2161 agcttggatc ttatcagtct gagctttaat taaatcagtt ctgtattcat tcatataaag
     2221 ttcattcatt tgcttgatgg ctttagctaa ctggttgcta atccgagtca atgaatcttc
     2281 aattgaaaga atatcatcaa ttttgcgatg tttttttctg ctaatctgca catcttgcat
     2341 aacctcacgc ttgatttcta gctttttacc atttttttca atcggagttt taatctttct
     2401 tagctgctgc aatcgttcga cttcttcatc atttagtcca gcttcagctt gttggattct
     2461 tttcatcatt cgaagttgtc ttattttaag aagtcgtatt tcttcggaca aagcaaaaga
     2521 aggatcatca ttcaaactag aatagatgtc cttctcatta tcggttaacg tgtcaaaaaa
     2581 tattgtttca tactcacctg ttttcaaagc gtttttatta cccactggcg gagaagctcg
     2641 gctatttcct ttgttacctt tagcattttg attaccaata ggagcgccac cttgattagt
     2701 aacgttactt ttgccattgg taacgttact aatcaattca gcgctccatt tgtcttgcga
     2761 tttccatttt ctaacctgag aatctgaaac atttaattca gatgcaattt cctttaactg
     2821 cttttctccg ttggattcta accaaattct tttggcttca tcacgtcttg gatcacgttg
     2881 tcttgccatt caatacacac cacctcacat tctgtttagg ttgagttttg ttttctaatt
     2941 ttcaatatct tttagcatta aatcagcttc aatcaatatc tttaaatcgg aaactttgtc
     3001 taacttgatt tgtcctgatt ggagattttt aagccatttc ttgcattcta atccctcaaa
     3061 ttcttgttaa tattattttg aatgtttggt tcatcataga aaccatgacc gcaataaacg
     3121 agcttgcacg catcaatctc acatggttct gcttctctcc ccatttcgac aatagagtac
     3181 ttccctttga tttgtacaga acgcacaaca cgcactgaac aatcatcaat ggttcgagga
     3241 tattcattag ttagcgatac ataccagtag tttctcatta tgtagcctcc tttatgtaaa
     3301 gaaaaagacc actcaaagag tgatctaata tgtaatgcac aggcagggac gtttccgatc
     3361 ctgtgcttga gacatttgac gattcatttg taccgaatcc caaatctcaa tctaacctaa
     3421 ccagttatgt aatagcaacc tacacgatgc acaaaacgcg tacgaaattg cgcaccccta
     3481 tatttttaaa ccgccgatgc ctcggttgcc aaagtcactg gcaaggaatc gaaccttgca
     3541 tggttgccga agcattgacc tagcacacat gcttagcgtc taccctttcc gccacagtga
     3601 cactataaaa ttattcttgg ctgctactat tttttatttt gcccattttt aaatccaatc
     3661 atatatacat taagacaaag cgcaaaaatt gaaattatta atggaatcat ttctcttcac
     3721 ttacctttag ttatcgtgtg aataattaaa aaaacaatag acagcaacgg atgatagata
     3781 ataagaacaa tttagaagga gttgaaattc acatccttat tcttaatatt tccgttgctg
     3841 cctatcgaag cttaattaaa cgatgaggga gatttcctcc cttacatttt attttgtcga
     3901 tcctgtttcc taatttttcg acactatcat aatacaacgt tgaataggta agtgattggt
     3961 ataaaaaagg tataaaatgg aaaccaaatt ggtaataaaa gggtataaaa agtgtaaaaa
     4021 ctggctactt gaaagcaacc agttctaacg atgaagcaaa ttggatgata attctgtttg
     4081 attctacttt aaccgattct tcgctagtat tattcctttg agcagttaca taaatgggca
     4141 gaccattgat ataacgatca tagaatatct tcttgcgcct ttcagtcaca tcaggcttat
     4201 gcggatgctg tatcgctgaa tagcctcgaa caaacaattt atgcagatac tcaaattctt
     4261 cctgtgcttc ttctttatcg atcagcattc tttctgcttc aaatatatga tcagctgtag
     4321 aaggtggaac caaggaataa gatgctgtca cttttggttc tcgatgttgc cctactctac
     4381 atctagctga tagatatgct gaaagaaaca cagcgacatt atgttttgtg cgttccatat
     4441 caacatcttt cgcgcttggt gtctcatatt tctttacatc gaaaagtacc atcctctgat
     4501 tcccccaatt atgatataat acttatgtcg gaaatattat tcatagtcgg aggaatccga
     4561 ctttttttat tttgtacgtg aaatgagttc tcctgattta tacgcctcag caaattcaac
     4621 caaagctact gccttcattc tctcgatagt tctatcggaa tatcctaatt tttctcctat
     4681 ttgatacatt gacatcttat taggaaaaca ataactataa taaagtacct ggaaatggat
     4741 tctacttagc cgtgttaatg cttcgataac cgaatctcga gtacttatcg cttcaatgat
     4801 ttcttcgttt tgactgtcaa ttgtgaattt caaatttttg gtaacagtca tcacggaaaa
     4861 gtctattttc actggtccga ccaaacgttc taaatttctg tatcttttta gtaatcgtct
     4921 agcatttttc cttgtttgct taatatctat attctctaaa atgttcacaa ttgcctcttc
     4981 tccttccagt tgtggaataa cgctcattgt tgtaggatag ttgtaaaaaa gcaaaattaa
     5041 gattttataa cttccatgtc caccaatctc accactgcta aattctcttt gcttttcgct
     5101 aaccacttat cacattccat cgtgttttca atgcgaatga ttgctgagtg attatagacg
     5161 tgttctacat atccacgaaa tggatagatg aatccttctg cttcgcagcg aaccatgtca
     5221 ccgactttga attttggttt cttacgtgtt ttaggattct ttgtcggcat atctagcatt
     5281 aaaccgccga tgccgtggcc gctggagtaa aatccgtctt ttagtttcat tccgcttcct
     5341 cctctttagc tgtcaattac ttactcgcaa gtcactaaaa tggctacttc atcgataaag
     5401 tttccgtctt cgtcttccat tccttgctga atgctaatgt cggtaatctg cttaaaaaat
     5461 tcgttgtcat agccttgtgc gtttatcact acttcgacgt ctccgaaaac ctctttgaga
     5521 ctattaattt tatttactac ttccgatgcg tccatgacta cacccctttc cagttaaaaa
     5581 tctctgtcgt taatagacta ctgttcaaag tattcatctt cactcatcct tctgcctcct
     5641 ctttagttgt cattagtgga ctaaatacta cctgtttgaa aaccataatg tgcagtttca
     5701 acgccgtcat aatcacttat ttcccactca gccccatcgg gtatatcaac gactttaaga
     5761 cttgcatatg gtccactggc ctcacttcct agtttttcga caacagcaac gaggtcctta
     5821 tcacctctgt ttgaatcact tgaaaaactg gaatcatcag caattccgta cagtcggtca
     5881 atttcgctat aaattcctgt aactttgtcg attttcgggt cttttttgaa atagtagata
     5941 aaatccatcg aacctagctt ttggcctgtg tatctcgtgt attcatcatc tgagtcatag
     6001 ctaaagttat tgatgtatgg gaatacttca atattttttc ttttaaaaat ttccattttt
     6061 gcttcatggc tcaacccaaa gccaccaaaa catttattta atacaatctt cattactttt
     6121 cctcctttgt ttcctccgtt agctgaattt tttcataaac acaagccaat gtgttttgct
     6181 tcgtttatta ccaaacaatg gctgttctcc aatagcctct aacacatcag aaagtggtat
     6241 ctgttcctcg ttccacttaa aaattaatgt tcctgaaggt cgtaaaactc gcatacactc
     6301 tgcaaaacct tgcctgatgt cttctggcca aagttcgtct aattttccat attttttggc
     6361 aagccatgag ttttcgccga catgtcttaa gtgtggcggg tcaaatacaa ccatatcgaa
     6421 cacatcatct tcaaaaggca tatcgcgaaa gtctgcgacc acatctggat caacattgat
     6481 cacatgacct gttgataatt cttcatactg ctgacgacaa tccataaatg ttacgtcaga
     6541 attgttctta tcaaaccaga acaatctact accgcaacat gcatctaata tttttggcaa
     6601 ttactctacc tccaataact ctggattctc gtagacgtta cctaaaacag tgagaaatct
     6661 ttctgtatcc cgataaataa ttagcttact tatcttccga accattcgac tctcttgttg
     6721 agataattcg tccatgtcac gatcacctat aatttgcaac attatctgac gacagattct
     6781 tttttcttgt ttagtcaaat tcatgcgatc gctcctttga ctggttttat gcgcttgtct
     6841 gctgtttgag aaaataccat cgtaaatcca tcagagttga caaacattct tgatattgtt
     6901 ctcgtaccgt aagctttttt cagttcttca ccaagcaagt tcgttgtgat aatcgttgct
     6961 aggttctgtc gtgcatccaa gaacgaattt agcgtgttta tgccaaacgc tcgactatct
     7021 gatgcatctt ttcctagttc tgaaccgata tcatcaatga tgactaaatc cgctgttttg
     7081 atatctgcaa tcaacgatcc ctcaatcgtt tttctgagtt caggattgtt gtacgaaaat
     7141 ttgatttgat ccagcatttc ctgtaacccg ataaataaga tttttttatc gtagtttgat
     7201 cgcttcaaga cttcccaagc cgctgccatc gctaggtgac ttttacctgt tccttgtttt
     7261 ccagttaaaa caaggtgact tggatttcct aacaagactg aattaacaaa gcgtttggtt
     7321 acttcaaccg cttgtcttgt ttcttgatcg acaatttgat aattctgcaa tgtgcaatca
     7381 aacaacgctt gattaggcac aacagaacca cctttgaaaa aattgattgc tcttgctttc
     7441 aggctctcgt tgtaaattcg ttctgtttgt aagtcttcct gaactcgcaa tgctttatac
     7501 ccacattgca tacaggttgg cttacaacgt tctgagccat cagggttctt cgtacgccat
     7561 ccatacaaag gctgtccgca ttcaggacat tcgccacgtt ggacaagcac tttctgtatc
     7621 agcttttcca tgatttcccc aacagtttcc atgcctacgc ctcctctcaa ataggcaact
     7681 catctgtgct aaatttctcg tattcaagcg ttttagtttg ttttgtttta taatcacgat
     7741 ttgcttgcgc tgccattgtg tcgtattttt ctcgcaattt tttagcagat aaaatattcg
     7801 atgcccaaaa cacattgtgc tgactccatt caatcattcc ccgtacctgg ttttcggtcc
     7861 gcttgtcgat ctcgatcatt ttccgaatgt catctgccca actttgcaga ttcggctttt
     7921 tgatttcctg attctgacaa atctgtttga ataactcctc cgcgagaatg tagtaaactg
     7981 agtcggtgtc gtaaacacgc tttttgcgtg gttgcgacga tgatgtttta ttattctttt
     8041 cattcttata attcttttca ttcttgtttg tgtgcacttg ttgttcactt gttgtgcgtt
     8101 tgatgttcat ttgttgttca ctgctttggt acaaagacca gttttttatt gatataacgc
     8161 tgtatttcgt agttgatttg atgttcaaca ttccgttttt ttcaaattgt tttagccatc
     8221 tccatacaga accgctgttc acttgatgtt cacgtttcac gccttcgttc atctcagatg
     8281 tgatagcatc gcgccctgtg acgaattctc cgctgttcac ttgtatctct tttccattaa
     8341 aaagtatctt gcgattctca tgactcgctt tcatcaaaca caagttccat aatttataca
     8401 tataaggatt ggtccaaacg aatgaattca tcacttttcg atgtaatttg acatatcctg
     8461 tgttcattcg tatttctcct ctagaaaagc aagggagaaa gctccctcat tatttgttta
     8521 acggcggatt agatgcatca aataatccag tttgtacatc ttcattttct tcagatataa
     8581 cctctgcttc ttttctttca ggaatatctt cttcaacttc tgtttcagca ataatgctgc
     8641 cgtcttcttg aaccctttgg actctctcat ccgatgtggt ggcttcttgc atttcgatgg
     8701 acaagattcc ccatttagaa agaagatttc tcaaaacagt ttttcgtgcc attgcattgt
     8761 aatcagatgc ccacacacca cttaactttg tcttatcgcg atctttattg ttagcaatcc
     8821 gatgagcttc gatttcttgt ttggtccaat agacagtttt tttgaatcca ttcagtaact
     8881 cgaaatagcc aacatatcca atgacttcat cagacgttct accatttgga tcaaactcaa
     8941 actcttctgt cagtcggttc cagcttttta gttctccttc gtaaacttcg atcacattta
     9001 atgctttgta tttacctgat cgttgggcta attggatata tcctttatag ccaagcatga
     9061 attgagcttt cttttcccat tttcctgttt gcttgttttt actattgaat ggaactaaat
     9121 atgcataacc taaattctta tctagcccaa gatttaatgt tgcagcagtt aacgcaccac
     9181 tcatgataga catcggttca ctatctgcaa gataactgtc attagataca agagtcataa
     9241 cattcgacat aaaagcatta gcattgtcat gaagcacctc ttcgaacttc tttctcattg
     9301 ttggtgtatt catcaaagct ttaagtccta attgacctgg tgcaacttgt ttctgtggct
     9361 ttgctgccaa ttgatttttt aacgattcat ttgttgccat attatttgat ctccctttcg
     9421 gttagccttc ttgattcagt aacgttataa atctcttcat catttgcgac atctggatat
     9481 ttctctgcta gtttcttcga gttcatacgt ctcgtacgga caaatttcca actgatgatg
     9541 tttttttgag tgataccgat actggcttca cgtttaccta gctcgctgat aatctcgttg
     9601 tctacttgac ggatagctga ttcaatttct ttcttagtcc gtttgagttc gtttttctgt
     9661 tcaactagtt catcgaaatg aattggtaac gtcgtttgaa cgtcttcaac atctgcatac
     9721 ttctctttca agaaatcagc tgtcgcttga ctaccatcaa taattggctc gatacctcca
     9781 agaacgtttg tttcccaaaa ctctactaat tgttcagtga ttgtatcgat cagctcttga
     9841 tctcgttcaa tccgcttcca gataaatttt tgaccaccga ttaagacagc gatgtaacaa
     9901 tagtctttgt ttagaacgtt catgtaatgt tgaacttgac agagatagct aagcggtacc
     9961 tcttcgcctt cccattcttt accgagaaat tgattggctg ttttgcattc cagaatggca
    10021 ttttctccca ctacttcccg atcaatattt gctcttaaaa atggatgtaa tggatgttca
    10081 aatacttggt ttcttctgcg tacttttttg cctgtacgtt cctgaaactc tttagcaacc
    10141 acttcttcta aaacattacc ccaataagcg ggctcacttc ctgattcttc aagtactact
    10201 tgtcctgttt tttctagcca tagttgataa ggagatttcc atttattcaa tcctaaaatt
    10261 gttccaacat ccgaacctcc gatgcctttc ttacggtctt caagccattc ttgatggctc
    10321 atttctaagg tagatttact catcttcatc ctcctcatca attggtgctt catatggtgg
    10381 tttagcataa tcaggatcag ttaaatagtt gtcaagattt gctaactcgt tcatgtttaa
    10441 cctcttttct gcttgctaac acgcaaagca caagcatggt aattgcgaat aacaagccaa
    10501 agattacata acttttcgct acaatcacta acgtgaaaaa taaaataatg agtagatcaa
    10561 ttgttttttc attcatcatt gaaattcccc cttgagaata cgagtcatca catcagctaa
    10621 atcttccttg ttattaataa caaaagtatg atttgaaggt tgattttctt cttcattttt
    10681 cgcttgttcg taagcatcca cagcaattct acgcataata tgtgctgaca atgggagatt
    10741 ttcatctaaa tgttcttctt gcatagcaag acaaagtaaa atttctgttg tctcaccata
    10801 aacgagactt ttaagttccc cttctttttg ggctgtacaa ataatagaaa caccttgctt
    10861 ttggcattca cgtttcaatt cttcgataag attttcaatt ttcttattca tgtgatataa
    10921 ttctcctagt tttataaatt tgtatgtgac tctttgctgg ctggcagagt cactttttta
    10981 tttgttgcca tgctttttgc ttttcgatat gttgcttgct taaaataata gggcggctat
    11041 ttccccaccg attatcagca atcactttac cgatttttag cgcttcttct cgtgccatag
    11101 ttgctccttt cttttgaatc aagcagattg attaaaacca tcaatgctgc gaacaagctt
    11161 cccccgataa tactttggtg cgctacaatc actaataacc ccaaaatgaa tcctataaaa
    11221 agtgtgtctg tcttcttcat aatctaatct ccctattttt tatttctagc attctcaaat
    11281 cctcaagttc agaagcaatt agttcagctt gcctatctga tagctcatcg gcttttctaa
    11341 gcgctgcacg gtcatcttgt aattgtttcc tgcgttgttt aatcaaaccg agaatttgat
    11401 gttcttgttg caaggtatat gccatatcac ttctcctttc gtttattgaa tttctccatt
    11461 tctatttgat aaaatatttc tatagaaatg gaggtgaaaa ctatgggaaa aaatcaacat
    11521 gttgtaccta attccaatgg tggttggaat gtcaaaggtg ctggaaattc taaagcgact
    11581 gcacatacta atacaaaatc agaagcagta aaaatcgcta gagagatttc taaaaatcaa
    11641 ggatctgagc tatttattca tggcagagat ggaaaaattc aaagccgtga tagtcacgga
    11701 aacgatccac acccaccaaa aggttaatca tatttaggag ttaatcgaat acgataaccg
    11761 tcagcaggag ttgcatcggt caatgtaact tctgctactt ttttcccttc ttctgtttca
    11821 ataattaatc ttgtgtaatt gttctcattc aaaatcccta tttttgattc ttccttcatg
    11881 tcatccctcc ttcgtctctc gccgtcacca cctccaagaa gaggagttgc caaaatgaaa
    11941 aaacgtggac tttggcaaac ttgaaaggag agcagcaact cctcttttca gaagtggtga
    12001 atttagattt tttagctatt aacaaacagc tctaactcta aatcgatttt tagaacgcca
    12061 tgttctaaac tgttcatata aatcgacatc aattgttatt tctttggaag taactgcgtt
    12121 atacccctca ctaaaacgtg attttttaaa ttccttcatt cttctttgat aagtactttc
    12181 agaatactta tatttctctt taaactccgc tttgctcaaa cgttgtacca tttttacgcc
    12241 tcctgttctg atggctgatc attagaatct acttctaact tgatatccaa aagttcttcg
    12301 atttgtttta aacgttcctt aggttttctt ttttcgatga agatatctcg taaataagta
    12361 attgatatac ccattcgtcg agctaactct ctttgactcc aacctttctt tactaaagca
    12421 attaatactt gctgttttaa agtcatgctt cataccccct tatcgaatat tttctaatat
    12481 ttttgtctaa taagtttgac aaaaactgca aatatgctat tatgtaagca tacaaaatac
    12541 agcgcacaaa aagcgagctt actgatgtct ggggagacta ttagtttatt gcttttagtc
    12601 tttgtatgcc gtaaatatct tgcttacaaa actagtataa ctgcaaattc tctatttgta
    12661 aatagataat ttgcagaaaa atactttttt tgtcggtatt ttgaaaggaa tgcatatcct
    12721 atgctattag accgtataaa aatcttatta aaagaacgtt caatgacgtt ggcagaactt
    12781 gaaagaaagt taaatttcgg aaatggaagc atgtctagat ggaataaaag tactccatct
    12841 ggagataaaa tacaaaaagt tgcagattac tttaatgtat caactgatta tcttttagga
    12901 cgtactgata aacgccatta ttatgattta acagaaaaag ataaacaaga tattgcttta
    12961 caagctgaaa aattacttga aggcttgaat tcagatgctg aaacaaatta ttatggtgaa
    13021 cctttagacg atgaagataa ggaaaaatta tacgatgcca ttcaacttgc acttgcctta
    13081 accaaagtta aagcaaagaa aaaattcact cctaaaaaat atcgtcaata ggtgggcttt
    13141 ggtatgcatt taaacaaata ctggatatta gaagaaatag aaaaattatt tgtagagttt
    13201 ggatctgatg cgattaatcc atataaaata atcaaatatt ctgatataga tttaattgaa
    13261 actgatttag ggaataaaac attaggacaa acgatacaca ataaaagatg ttatgtgata
    13321 ttaattaatc aaaacctatc accttctatg aaaagatttg tattatggca tgaaatagcc
    13381 cacgttagac tccataaagg tatttgcaca gcagctttta gagcaaacaa ccttagttgt
    13441 atgatttatg gtatagaagc tgaagctaat atgtttgcaa ttgaaatgtt aactagaaca
    13501 atagatgaac aagatttatc tgatatgagt cagttccaaa ttattgaata tttagggctt
    13561 cctcataatc ttgaacattt agtattacaa cattaaaaat aaaaaagccc atgctttcac
    13621 atggactctt tcctcatttc tgagtaaact ctacaataat tatatcaaag aaatgaggag
    13681 ttttgaatgt tattattgat tggacttatt ttattaatta taggaatcac taaactgtcg
    13741 aaagctaaca caataaaaaa agaagcttat gaattagaac aaaaaaatga tgaaaaacta
    13801 aataaattac atgatagaga aagatttata ttaagtaagg aagaaacact atcagattta
    13861 aataaacaaa ttactatgtt aaaaaatgat attattttat taaaaaaaga gaaagaagat
    13921 ttaaatgatg aaataaatca aaagtatgat gaaacattaa aaaatgtcta ttcttcactt
    13981 atcgtttcaa atatagaaga aacttcatct tctgaaataa
//
LOCUS       DASJPH010000074        13794 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJPH010000074 DASJPH010000000
VERSION     DASJPH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.074"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="U3995_002064"
     CDS             140..460
                     /locus_tag="U3995_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797370.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="U3995_002065"
     CDS             912..1112
                     /locus_tag="U3995_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1797371.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="U3995_002066"
     CDS             1259..4048
                     /locus_tag="U3995_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1797372.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="U3995_002067"
     CDS             4095..4622
                     /locus_tag="U3995_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1797373.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="U3995_002068"
     CDS             4643..5833
                     /locus_tag="U3995_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1797374.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="U3995_002069"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="U3995_002069"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1797375.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="U3995_002070"
     CDS             7966..8640
                     /locus_tag="U3995_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1797376.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="U3995_002071"
     CDS             8637..8978
                     /locus_tag="U3995_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797377.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="U3995_002072"
     CDS             9008..9367
                     /locus_tag="U3995_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1797378.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="U3995_002073"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="U3995_002073"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1797379.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="U3995_002074"
     CDS             12286..13752
                     /locus_tag="U3995_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1797380.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4579 a         2392 c         2938 g         3885 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct actt
//
LOCUS       DASJPH010000075        13739 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJPH010000075 DASJPH010000000
VERSION     DASJPH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.075"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..1810
                     /locus_tag="U3995_002075"
     CDS             179..1810
                     /locus_tag="U3995_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1797381.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1872..2513)
                     /locus_tag="U3995_002076"
     CDS             complement(1872..2513)
                     /locus_tag="U3995_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1797382.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2699..2775
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2795..3103
                     /gene="rplU"
                     /locus_tag="U3995_002077"
     CDS             2795..3103
                     /gene="rplU"
                     /locus_tag="U3995_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1797383.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3130..3474
                     /locus_tag="U3995_002078"
     CDS             3130..3474
                     /locus_tag="U3995_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1797384.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3487..3780
                     /gene="rpmA"
                     /locus_tag="U3995_002079"
     CDS             3487..3780
                     /gene="rpmA"
                     /locus_tag="U3995_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1797385.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4214..7021
                     /locus_tag="U3995_002080"
     CDS             4214..7021
                     /locus_tag="U3995_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797386.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7212..7703
                     /locus_tag="U3995_002081"
     CDS             7212..7703
                     /locus_tag="U3995_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797387.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7806..8795
                     /gene="manX"
                     /locus_tag="U3995_002082"
     CDS             7806..8795
                     /gene="manX"
                     /locus_tag="U3995_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1797388.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8828..9631
                     /locus_tag="U3995_002083"
     CDS             8828..9631
                     /locus_tag="U3995_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1797389.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9652..10566
                     /locus_tag="U3995_002084"
     CDS             9652..10566
                     /locus_tag="U3995_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1797390.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10649..11023
                     /locus_tag="U3995_002085"
     CDS             10649..11023
                     /locus_tag="U3995_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1797391.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11162..11407
                     /locus_tag="U3995_002086"
     CDS             11162..11407
                     /locus_tag="U3995_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1797392.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11579..12643
                     /locus_tag="U3995_002087"
     CDS             11579..12643
                     /locus_tag="U3995_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1797393.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12889..13314
                     /locus_tag="U3995_002088"
     CDS             12889..13314
                     /locus_tag="U3995_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1797394.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13307..>13739
                     /gene="nusB"
                     /locus_tag="U3995_002089"
     CDS             13307..>13739
                     /gene="nusB"
                     /locus_tag="U3995_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1797395.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKE"
BASE COUNT         4365 a         2298 c         3030 g         4046 t
ORIGIN      
        1 ttttactcaa acaaggtggg ctagctctgt tggcattgct ttctattttc ttataaatat
       61 agaaaaaata agaatttatc taacaggaaa ctttgtgaaa aatattactt tatctcatga
      121 aatatgatat tctaatgaat ggtattttag aaaatttcat gaaaaaggag ttagataggt
      181 gttcaaaaat tatgtttcct cactaaaaaa agaattttca ggttatgatc tttcaagatt
      241 tcagaaagat cttttggctg ggatcacagt agctgctgta gcattgcctt tagccttggc
      301 ttttggggta tcaagcggag ctgatgcggc cgcaggtttg attacggcta tcattgccgg
      361 tatcgttatt ggcggtttat caggcggttt ttatcaaata tcaggtccga caggtgcgat
      421 ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat tgcttgctac
      481 ttttttagcg ggtctatttt tacttgcagc aggacttttc cgattaggaa cactgacttc
      541 ttttatccca gcacccgtaa ttacggggtt tacttcaggt attgctatca ttattgcatt
      601 aggtcagatc gataatttat ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact
      661 aagtagttat caaacgatag gatttccgat aaacacagcc tctttgatca tgggagggct
      721 agtcatttta gggatgttta tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct
      781 gctggctatc attttggcaa cagcagttat gctattgttc catttaccag tagcaactgt
      841 gggtaagatt ccacaaacat tgatcagtag taatcgtttg aatatgggag attttagctt
      901 ttctgccttg caacaagtgg cagttccagc tattagcatt gctttattag gaatgattga
      961 aagcttgcta tgtggtgctt cggctggccg aatggcaaat aagcccttag atagcaatca
     1021 agaattaatg gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg ggattcctgc
     1081 tactgcggcc atcgcccgta caagcgtggc catcaaatct ggtgcacaaa ctagattagc
     1141 tggtatgata catgcagttg tcttactgct ctccatgctt gtttttgcgc cagtgatgtc
     1201 acagattcca atgccagcac ttgcaggtgt tttgattgtg acggcttggc gtatgaatga
     1261 atgggaaacg atcaaggagc tatttactaa taaatattgg tcagcagttc tattgtttat
     1321 ccttacgatg atttgtacag ttatctttga tttaagtatt gctatcgtga tcggggtgat
     1381 cggcggctgt gtattttttg ttgtcaaaag cgctgctatc acgatttcat tagaaacgat
     1441 cgattggcaa agaatggggc tgccagaaac ggaaaagctg aataactgga cagtagtgta
     1501 tattagtggt ccactgtttt ttatgtcttc agaaaaactc aaaaataaat tggaagaact
     1561 aaatcatcac gatggtgtca ttttctccat gcgaggcgta ccaagtattg atttgacggc
     1621 acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa cgattatcta
     1681 tacttccttg cagccagaag tagaaaaaca actttctcac ttgtgggaac atacattcac
     1741 gcatcaacat caaacagttg ctcatgcgtt gacagcttta catgcgaaat atactgcaat
     1801 tagccattaa aacaatgaaa aagaacagac aagactgtaa actgaagatt ttatggtctt
     1861 gtctgttctt tttaatattg ctgtaaaagt gaagctagta cttgccagtc ttgatggata
     1921 acaggtacaa gggagcgatt gtagaaaata gctgctggat gaaaaagtgg aagaattcga
     1981 tagttttttt ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa gacaggccct
     2041 gtatagagtt gaccatgatt tttggtaacc gtatattctt tgccaagtaa acgttgaaga
     2101 ccaatgtttc caagtgtcgc aattaatttt ggctggacct tcatcagttc gtaatccaat
     2161 attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc gatttggata aacagtttct
     2221 gactgttgtg tccgtttatt gatccgatga gtcacgcgat acgggcggct ccttacagca
     2281 ctggtgatat acacctcttc tcgtgttaag ccagttgaag aaagtgcctg catcaattct
     2341 tttccagctt gaccgctaaa aggaacatgc gtatcaatct ctgttttccc tggggcttct
     2401 cccacgagca tcagtttagg atgaagaggt ccttgcccag cgataaatcc ttctacagga
     2461 cggccgttca ttcttaaaga agcttcttgt atcagttctt ttgggtattc catttatgct
     2521 cctccttttt cttttattat aagaataaaa gaaacacaat gcgactattt tatttggtag
     2581 aactgtgagg atagaaaagc agaagtattg cttgaatttt tgttttactt tttgatttta
     2641 aatttagttg actaacggtt gctgttctgg taagctagta ctgttgtaat tgtggactcc
     2701 acagctacaa ccgctcagaa caagtaataa gcacagcata tgctgtcact tcggatggcg
     2761 agtctaagta tatcagaagg aggtgcttta tagcatgtac gcaattatta aaactggtgg
     2821 taaacaagta aaagttgaag tgggacaagc aatctatgtt gaaaaacttg acgttgaagc
     2881 aggagaaaaa gttgtttttg acgaagttat cttagtaggc ggagaatcta caaaagtagg
     2941 cgctccaact gttgcaggtg caactgttga aggtactgtt gaaaaacacg gcaaacaaaa
     3001 gaaagtcgtt actttcaaat acaaacctaa aaaacattca caccgtaaac aaggtcatcg
     3061 tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa
     3121 ggcctatata tgatcaaagg aacatttaag cgaaatgacg caggtcagat cgtttcattc
     3181 actttgaccg ggcatgctga tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt
     3241 tctgcgttag cgatcagcac tgtcaacggg attgcctcat tagccggttt tgagccgatt
     3301 gttgaaatga atgaagaaga aggcggttat ctttataccg aagtcacttc aggaatgaca
     3361 caagaacaaa acaatattgc tgagattcta ttagaaaatc ttttattagg attacaatcg
     3421 atagaagctg aaaactcaga atatatccaa attaaaacaa ttaatgataa ataggaggtg
     3481 cagaccatgt tgttatctat gaatctacaa ttatttgccc acaaaaaagg tggcggttct
     3541 acatctaacg gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa
     3601 acagttacag gtggatcaat tctttaccgt caacgcggta caaaaatcta tccaggtgta
     3661 aacgtgggta tcggtggaga cgacacttta tttgcaaaag ttgatggcgt agtacgtttc
     3721 gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc cagtagctca agaagcttaa
     3781 ttgaatctta tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata
     3841 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
     3901 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
     3961 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
     4021 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
     4081 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
     4141 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
     4201 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
     4261 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
     4321 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
     4381 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
     4441 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
     4501 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
     4561 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
     4621 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
     4681 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
     4741 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
     4801 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     4861 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     4921 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     4981 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     5041 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     5101 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     5161 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     5221 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     5281 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     5341 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     5401 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     5461 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     5521 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     5581 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     5641 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     5701 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     5761 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     5821 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     5881 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     5941 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     6001 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     6061 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     6121 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     6181 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     6241 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     6301 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     6361 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     6421 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     6481 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     6541 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     6601 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     6661 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     6721 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     6781 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     6841 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     6901 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     6961 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     7021 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     7081 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     7141 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     7201 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     7261 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     7321 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     7381 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     7441 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     7501 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     7561 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     7621 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     7681 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     7741 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     7801 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     7861 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     7921 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     7981 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     8041 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     8101 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     8161 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     8221 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     8281 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     8341 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     8401 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     8461 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     8521 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     8581 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     8641 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     8701 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     8761 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     8821 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     8881 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     8941 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     9001 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     9061 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     9121 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     9181 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     9241 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     9301 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     9361 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     9421 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     9481 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     9541 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     9601 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     9661 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     9721 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     9781 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     9841 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     9901 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     9961 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
    10021 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
    10081 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
    10141 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
    10201 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
    10261 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
    10321 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
    10381 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
    10441 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
    10501 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
    10561 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
    10621 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
    10681 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtaa
    10741 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
    10801 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
    10861 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
    10921 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
    10981 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
    11041 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
    11101 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
    11161 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
    11221 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
    11281 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
    11341 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
    11401 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
    11461 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
    11521 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
    11581 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
    11641 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
    11701 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
    11761 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
    11821 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
    11881 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
    11941 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
    12001 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
    12061 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
    12121 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
    12181 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
    12241 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
    12301 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
    12361 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
    12421 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
    12481 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
    12541 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
    12601 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
    12661 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
    12721 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
    12781 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
    12841 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
    12901 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
    12961 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
    13021 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
    13081 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
    13141 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
    13201 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
    13261 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
    13321 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
    13381 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
    13441 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
    13501 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
    13561 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
    13621 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
    13681 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaat
//
LOCUS       DASJPH010000076        13614 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJPH010000076 DASJPH010000000
VERSION     DASJPH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.076"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..144)
                     /locus_tag="U3995_002090"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="U3995_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="U3995_002091"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="U3995_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1797396.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="U3995_002092"
     CDS             complement(1716..2498)
                     /locus_tag="U3995_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797397.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="U3995_002093"
     CDS             complement(2563..3399)
                     /locus_tag="U3995_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1797398.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="U3995_002094"
     CDS             complement(3417..4826)
                     /locus_tag="U3995_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1797399.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="U3995_002095"
     CDS             complement(4840..6663)
                     /locus_tag="U3995_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1797400.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="U3995_002096"
     CDS             complement(7506..7832)
                     /locus_tag="U3995_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797401.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="U3995_002097"
     CDS             complement(7878..8240)
                     /locus_tag="U3995_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN1797402.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="U3995_002098"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="U3995_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="U3995_002099"
     CDS             8619..8954
                     /locus_tag="U3995_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797403.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="U3995_002100"
     CDS             complement(9030..11027)
                     /locus_tag="U3995_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797404.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="U3995_002101"
     CDS             complement(11125..11400)
                     /locus_tag="U3995_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797405.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="U3995_002102"
     CDS             11632..12366
                     /locus_tag="U3995_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797406.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..13547
                     /locus_tag="U3995_002103"
     CDS             12405..13547
                     /locus_tag="U3995_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1797407.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4373 a         2681 c         2089 g         4471 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    13381 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    13441 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    13501 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    13561 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tata
//
LOCUS       DASJPH010000077        13429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJPH010000077 DASJPH010000000
VERSION     DASJPH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.077"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U3995_002104"
     CDS             complement(<1..914)
                     /locus_tag="U3995_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1797408.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U3995_002105"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U3995_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1797409.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="U3995_002106"
     CDS             2006..2176
                     /locus_tag="U3995_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797410.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="U3995_002107"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="U3995_002107"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1797411.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="U3995_002108"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="U3995_002108"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1797412.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="U3995_002109"
     CDS             4440..5321
                     /locus_tag="U3995_002109"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1797413.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="U3995_002110"
     CDS             5318..6034
                     /locus_tag="U3995_002110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1797414.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="U3995_002111"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="U3995_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1797415.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="U3995_002112"
     CDS             6751..7752
                     /locus_tag="U3995_002112"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1797416.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="U3995_002113"
     CDS             7749..9110
                     /locus_tag="U3995_002113"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1797417.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="U3995_002114"
     CDS             9070..10221
                     /locus_tag="U3995_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1797418.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="U3995_002115"
     CDS             10196..11365
                     /locus_tag="U3995_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1797419.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="U3995_002116"
     CDS             11540..12919
                     /locus_tag="U3995_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1797420.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="U3995_002117"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="U3995_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DASJPH010000078        13239 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJPH010000078 DASJPH010000000
VERSION     DASJPH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.078"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            206..1945
                     /gene="yfcC"
                     /locus_tag="U3995_002118"
     CDS             206..1945
                     /gene="yfcC"
                     /locus_tag="U3995_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1797421.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2102..3286
                     /gene="iadA"
                     /locus_tag="U3995_002119"
     CDS             2102..3286
                     /gene="iadA"
                     /locus_tag="U3995_002119"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1797422.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3397..3600
                     /locus_tag="U3995_002120"
     CDS             3397..3600
                     /locus_tag="U3995_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1797423.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3701..4363)
                     /locus_tag="U3995_002121"
     CDS             complement(3701..4363)
                     /locus_tag="U3995_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797424.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4376..5878)
                     /locus_tag="U3995_002122"
     CDS             complement(4376..5878)
                     /locus_tag="U3995_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797425.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6426..8105
                     /locus_tag="U3995_002123"
     CDS             6426..8105
                     /locus_tag="U3995_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1797426.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8232..8711
                     /locus_tag="U3995_002124"
     CDS             8232..8711
                     /locus_tag="U3995_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1797427.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8930..9610
                     /locus_tag="U3995_002125"
     CDS             8930..9610
                     /locus_tag="U3995_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1797428.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9618..10949
                     /locus_tag="U3995_002126"
     CDS             9618..10949
                     /locus_tag="U3995_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1797429.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10964..11926
                     /locus_tag="U3995_002127"
     CDS             10964..11926
                     /locus_tag="U3995_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1797430.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12037..12375
                     /locus_tag="U3995_002128"
     CDS             12037..12375
                     /locus_tag="U3995_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1797431.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12454..13161
                     /locus_tag="U3995_002129"
     CDS             12454..13161
                     /locus_tag="U3995_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1797432.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4234 a         2326 c         2895 g         3784 t
ORIGIN      
        1 ttttaaggaa aaacagagaa ataaaatact taatataaat ggaaagagaa aaaaataatc
       61 atacatagga gaaaaaaaac aaaatagaaa aaagtgattt tctaataaat tattcatgtg
      121 gaaatgtcat ttttactgta taattcattt gattatccat tttttattca tcaaaattac
      181 tgaaaaaaga aaggaagaaa taaatttgaa gaaaacaaat tcttcaggaa aaccgaaacg
      241 cgacttcact aaattatcga caccccatac gtatgtcatt atttttggtg tagtcatctt
      301 tgcatggata ctgacttttg tcgttccagc aggaaagttc agcacgcaag atattgaata
      361 caaagatgca aatggcgaaa caagtactcg tactgtttta cgtcaagatt ccttccgtta
      421 tgcttacgag ctggataagt cgtatgtgtt tgatcaatta gaagaactgc aagatcatcc
      481 agcagaacgt gaaaaactgg atgttccgga aaaaggattg gaaaaagtca ttgctgacgg
      541 agaaaaaaat cttacgcagg aaaaactaga tgaaatctcc ttgacagatg atgtgcttta
      601 cgatcagtac ggcgaaaata tctatgatac ttctaaaaaa ttgcataaaa cagccaaaat
      661 atggggaaca gatgattttg gcggctttgg tttcttaaat tttgtttttg aaggactagt
      721 atctggagat aagtatggat ctgctgtagg gatcgctgca ttgatccttg ttgttggagg
      781 tgcttttggt attattatgc gtaccggagc gatcgacgca ggtatctatg cgtttatcag
      841 taaaactaag ggactggagc gtttagcttt accgctatta ttctttgctt tttcattcgg
      901 tggcgctaca ttcgggatgg cagaagaggt aattccattc tcaatggtga tggttccatt
      961 tgtcattgcg ttaggatatg actcgatcgt agcagtaacc gtgacttatg tcgcatctca
     1021 agtcggaaat gcgacctctt ggatgagccc gtttagcgta gcggtggctc aagggattgc
     1081 tggaatcccc gtgttgtctg gtgcgacatt ccgattgatt atgtgggtag ttgttactgc
     1141 attagccgct ggttatatga tgatatatgc agaaaaaatc cgtaaaaaac cagaaagttc
     1201 gttgacttac aaatcagatg attatttccg ttctcatatc aaaaagacat ctgatgaaaa
     1261 taaaaaattc atgcttgggc ataaattgat cttattagag atgttagttg tccttgtctg
     1321 gatcgtttgg ggcgtgactc aaaaaggcta ttacattcca gaaatcgctt ctcaattctt
     1381 tgtgatggga ttagttgctg gaatcattgc tgttcttttc aaattagatg gaatgaaagt
     1441 caatgacatc gcttcttctt tccaatctgg tgcagctgat ttagctggta cagcaatcgt
     1501 agtaggtatg gccaaaggga ttcttcttgt cttgggagga tctgatgcat ctgttccttc
     1561 agctttgaat acgattttgc atgggatcgg gacggcgttg accggtgttc cagctgtcat
     1621 tggtgcttgg gcgatgtaca tcttccaaag tttgttcaac ttggttgtta catcaaattc
     1681 aggacaagca gctttgacta tgccaatcat ggctccactg gcagatttag taggtgtatc
     1741 cagacaagta gcagttcttg cttatcagct tggttcaggt tttatggacg cttttacacc
     1801 cgtatcagcc agcttgattg gtgtgctagg tgttgcacgg atcgaatggg caaaatgggc
     1861 aagattccaa atcaagatgc aaggattcct gtttgtttta ggaactattt tcatcatgat
     1921 tgccattgca ataggtctgc agtagtactg gagacctgta aataaaaaag taatccaaac
     1981 agtagataag gactccaaga accgtcattt tacggtaaaa tcaaaggagt aaagatccta
     2041 tcgtttggat tattttttaa gatcctatca tcagttatac ttaaagaaag aggaaaaatg
     2101 tatgaaaatc attcgacaaa ttgaagtgtt tgcaccagat tacttaggaa aaatggatgt
     2161 actgattgcc ggtgggaaga ttttggctgt agaagaacag ctagaaggcg gctacgaagg
     2221 agtggaagta gaagaacttt caggtgaggg aaaagtacta accccgggtt tcattgactg
     2281 tcatttccat attttaggtg gaggaggaga aggcggttat caaaatcgaa cgccagaagt
     2341 cacattgagc caattgacta cagctggtgt aacgacagtt gtcggttgtt tgggaacaga
     2401 cggagaaggt agagatatga ctgcgttgat cagcaaagcg aaaggtctgg aagcagaagg
     2461 aatctccact tatgtatacg aaggttcgta ccgattgccg gtaaaaacag taacagattc
     2521 tatcatcaaa gactttttga caattgataa aattatcgga atcggagaaa tcgcagtatc
     2581 cgatcaccgt tcttctcagc caagttttga agagttcgct cgtgcagtag cagatgctcg
     2641 tgtaggaggc atgttatccg gaaaagctgg gatcatcaat gtccacttag gcggaggaaa
     2701 aagaaagcta gagctattga cacgaatcgt agaagaaaca gaaattccga tcacacaatt
     2761 tttgcctact catgcaaatc gcacaccaga attatttgaa gcttgcgtgg catttgccaa
     2821 acgtggtggc acgattgact tcactgctag cgaagatcct gatttttggg agaagacaga
     2881 tggtgaagtt cgcttcagca aagcactgaa acgattgatc gaggaagatg tttctttaga
     2941 taatttcacg atgacctcag atggacaagg aagcttgcct tattttgatg aaaacaatca
     3001 tttcctaggg ttaggagtag gaagtgccaa ggcattgcta gtagggatca aagaagctgt
     3061 gcaaaaagaa agtatccctt tggaaatcgc cttacgagct ataaccagta atccagcacg
     3121 tatattgaaa ttggataaaa aaggaaaaat cgaaattggt gctgatgcag atctgtgtat
     3181 cttggataaa gaaacgcttg atattgatac agttatcgct aaaggagaaa taatggtcca
     3241 agaaaaagaa gtcaaagtat gggggacttt cgaaaaaagt ttttaatcaa cagctctaac
     3301 tatctttttt agaaaacgag cgttctgttt gtctgtatct gttgttgatg ttaccctgaa
     3361 ggtagatcaa taaacaaact aagaggagga agcacaatgg cagatttaaa aggacgttct
     3421 aaagatatga aagataaagt agtcggtgaa ggaaaagaag catacggcaa agcaacagac
     3481 gacaagagca aagaaacaga agggaaagca caatcactag gtgctgacat caaagaaaaa
     3541 gctcgaaatt taggcgacga agtcaaagac aagttttctc gaaaagacga aaaaaaataa
     3601 aaaccactta ttactaattg aatcaagcaa ataaaaaatc cgaaccatat ctgcgagaca
     3661 gatatggttc ggatttttta tttgatcatt atcctgtttt ttaaacttcg attttttgaa
     3721 attttttatt ttttagttca aagacttcat cacagctttt tgctacttca atctcatgag
     3781 tcacgatgat aatacagcga ttttgttcat gggcgatttc ttggaaaagt ttgacgatat
     3841 cttgtgaagt tgtttcatct agatttccag taggctcatc cgctacgatt agttgatgtt
     3901 cacagcagat agcacgaacg atagcgactc gctgctgctg acctcccgaa agtttggtca
     3961 cctgtttttt tgctaatatt tcatcaattc ctacacgagc cagtcgatct aaagcaactt
     4021 gtcgataatt ctcttgcttg gtcttagtga tttccattgc tgctatgacg ttgtctagag
     4081 ccgataaata aggaagcaag ttgtaagctt ggaaaataat cgagacatca tttttacgat
     4141 aacttcttaa cccgattttg ttcagtgaac gatcttgata gtataccttt ccttcttttg
     4201 gtgcatctag tccagaaata agcgaaagaa aagtcgtttt tccagaacca cttgctccta
     4261 aaatagcgta catttttcca gcttcgaaat tcagttctac atctttataa agcgcatctt
     4321 gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa catcaaattc ctcctttagt
     4381 ttaataggat ttttcttggg tttaatctta atatccctat agaagctaaa aagacagaaa
     4441 acagactgat tccaaatgct actccggcta atagtcccaa ttgtgcaggt ttgacattga
     4501 tttccaattc gctgacttga tcagaaacag caaatggatt attcgtggtt ccttgttgac
     4561 catcgcgttg accgccttgt tcttgtctgt tacctggcat ttgtccaccg ccaggagctt
     4621 gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag ctgctgtccc acggcattcg
     4681 cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag aatcatacaa atagccactt
     4741 cggtaaacaa ttggagaatg attttacttc tggattctcc taaagatagc aagacgccta
     4801 actcatgccg acgctcccga atggtaatca taatgatcag agtaagtatg ataataccgg
     4861 ctacagccac tagtaaaaca acatttttag aaaaacttgc cacgttgttt aaaggttcca
     4921 acattgactg atacatggaa tcatttgatt gtaagctaaa tgtatctgtg tcgatcagct
     4981 tctcagcttc tttgagaaac tcatccattt tatctggatc agatagagtg taaatagcag
     5041 aatctatcgt gttatcgtca gatgtcccat ttagttcatt tgcaaaagtg taagaagtgt
     5101 aaagtgtatt cgacggattc atgaagttga atttcatacc catactgccg cttgtttctc
     5161 tggattcata aattcctttg atcgtcattt cataagtggt atcttcatct tcagtactcg
     5221 tgattgtgaa agtatctccg actgccaggt catttgtttc tgctagagta gaatcaatga
     5281 ctacattgtt tgtgcctttg tcgctgtcat ctattccttc accatccgtg atttttgctg
     5341 ttccatcaga aaaattacta ttttgtgcag ttgctgacac accactgatt tgaaagtctg
     5401 cttgagtcat ttgcggattc ccgcccattc ctccagcagg ttcaccgcct gcatcagtag
     5461 tttctgaact gtcttcactt gaatcagaac tagtgatggc agttattcca tcttttgcca
     5521 tagctgaggc ggaactttca aaggaataac tctttacatt atctaattca gctatttttt
     5581 ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc tggatctgga cgttcttcag
     5641 tggtagaacc gcttgaagaa gtttgttcct gttttttaaa tgaagcttct cgattggcac
     5701 ttaaagtcac tgttgctcca acttcttttt gtgcttgatt cgctgcttct tctgcagcgc
     5761 ttcgaatcgt gaggccagct agcacaaaaa taagaatggc tgtgaacacg gcaaacaata
     5821 acaatgaacg tccccatttt acttttgtac ttttccatgc acgtttcata aaattcatat
     5881 tttgcatccc tcctagtaga tgcatttgat aatagacaac ctatcttaaa gctagcttaa
     5941 atcttatgct gtttgcacac catcaacatg aggttctgta tatataaaaa gtcactatag
     6001 ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc aaaccgacga agaagaaatg
     6061 aaagaggaat agcaaaagaa atcctagatg ctacagaggg atttttacgt gcatagttgt
     6121 tctaacaatt gctgatacta gcgatttagt ttatttgttg tataataatg gggaacagct
     6181 gaatacttat ataagtattc tggctatagc gtttcgcaaa agatccattt tgttaatgaa
     6241 gtatatccaa aataaaatgc ccatccaact gtcagaacgc ggatgtgctg gtaattttcc
     6301 tgctgattct ccataagttg agaaatagtg attttgcata tgcttcattg gaatggctca
     6361 tatatttaga taaagaagaa aatcgacatc tttaaacttg gatgtcataa gaagaggtga
     6421 agtttgtgag taccattaaa attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca
     6481 tgtatgtcgc agaagtgggc gaggatattt ttattttaga ttgtgggcta aaatatcctg
     6541 aaaatgagtt gttggggatc gacgtcgtaa tccctgattt tacgtattta gaagaaaaca
     6601 ttgatcgtgt agctggtgtc tttttaacac atggacatgc agatgcgatc ggggcacttc
     6661 cttacttgtt gtcaaaaatt tctgtaccag tattcgggac agagttgacg atcgagttag
     6721 caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt taaggatttc catgtggtag
     6781 atgagcatac agaaatagac tttggtgaaa cagtagtgag ctttttccgt accactcata
     6841 cgataccaga ttcagtgggg atcaatctga aaacaaaaga aggtagtatc gtttatacgg
     6901 gagattttaa atttgaccaa acggctatcc caatgtatca aacagattat gcacgattgg
     6961 cggaaatcgg caaagaagga gtcttagcct tactaagtga atcggcaaat gcagaaaatc
     7021 ctcagcctgt agcttctgaa gcccaaatag cagatgaagt atttgatacg atcaaatatt
     7081 gggaagggcg tatcatcgtc gcttgtgtag caagtaattt gcaacgagtg caacaagtat
     7141 tggatgcagc agaaaaagca caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa
     7201 ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta
     7261 cgttgaaaga catgaaaaac tatgatccag aagaactgat cattttagaa actggccgaa
     7321 tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga
     7381 ttgaagaagg agatttggtt tatatcacta ctacgccaag catcgcgatg gaaaccatcg
     7441 tggcgaaaac ggaagatatc atttatcgag ctggtggaac agtgaagttg atctctgaga
     7501 atatgcgcgt ttctggccat gccaatccta atgacttgca attaatgatc aatttgatga
     7561 aaccaacata ttttgtgcca gttcaaggag agtatcgtga attagctgct catgcagatt
     7621 tggctcatgc agtagggatg ccatacaaaa atatctatat tactggacgc ggagacgtgc
     7681 tggaatataa gaacaagcgt atgagtgttg caggaactac aagtgcagaa aatgtaatga
     7741 ttgatggatt aggcataggt gatatcggaa acattgtttt acgtgatcga aaaatcttgt
     7801 cagaagacgg gattttcgta gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt
     7861 caaaacctca aattacttca cggggattcg tttacgtaaa agctagccgt gatctgatga
     7921 aagaaagcag caatatgatt gaaacaatcg ttgagaaaca tttagaaagc aatgattttg
     7981 agtggagcaa attaaagcaa gacatccgag atcaattagg ccgttactta tttgaacaaa
     8041 caaaacgccg cccagtgatc ttgccagtca ttatggaagc tactcaacgt cgaaataaaa
     8101 aataaacaaa aagagtaaga tgaacctctc tatagagtgc tcgtcttgct ctttttttgc
     8161 gaaaaacaaa taatttttga atacagcatc cctcgcttta taataaacga tagaataaag
     8221 ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta tttagaagaa caaaagatca
     8281 gctatcaacc acttgatttt agtcatgtgg tagaagaatc tttcacagaa gaactacaaa
     8341 aaagagggat tgatccagct ttgatatgta aaactctcgt agtgaaagga aacaaaactg
     8401 gggtgatcat cgcattgatt cctttgaacg atcacttaga ttacaaaaaa acgagacaag
     8461 tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt tgtattaatg catacaggat
     8521 atccccacgg agcaaacacc cctattggaa taaaactcgc acatcctgat tattgttttt
     8581 tagcagacag ttctttaaaa gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga
     8641 gaagccttcg gataaaagta gccgatttag aaaagatcgt ccatcctatt tacgctgatt
     8701 taattagtta agggacgaga agagaagcta agcagagcag agacctcaaa cttgagatta
     8761 tatctgcttg gtacttggtg catcctgtat tatctgttac aattgctttg tacatactgt
     8821 actatcaatc caatgaaaat atatttagca gctatatttt ttcattgatg tgcaagtgat
     8881 gaaatcgttt ttaataaaaa agatacagag aaaacgcgag gtgtaaaata tgaaaattac
     8941 gtatcatgga cattcctgtt taacaattga aatggaaaat ggccaacgcg tgatgattga
     9001 cccgtttatt acaggaaatc cattgacaga cttaactgca gaagaagtaa agacagattg
     9061 gttgctgatt actcatggtc atagtgacca cataggagat atgattccta ttgcaaaaga
     9121 aaatcaagcc acaattatca gtattgttga gattgctgat tatgcgaaca gcagaggagt
     9181 agattcattc ggtatgaata tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt
     9241 cgtacatgca cagcatagtt caagctatga agtagacggt atcgttcagt atatgggcga
     9301 gcctagcgga attattattc aagcagaagg aaaaacgatt tatcatgcag gggatactgc
     9361 ttatttcagt gatctaggat tgcttgcaga agaattcgat attgatgtcg ctttcttgcc
     9421 gattggcgat aactatacga tggggccaga agatgcagca aaggctgcgc aaacaattcg
     9481 tgccaagaaa gtagttccaa tccattacaa tactttccct gtcattgaac aagatcccga
     9541 atcttttgta gagttactgg aagatagtga aggaaaaata atgagcgtct cggaaacgct
     9601 ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat tttagaatat attgaaagtt
     9661 tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag
     9721 gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg actagtttca acaattcaac
     9781 gagtagggac catccgtatc gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg
     9841 gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg ggcggcagga ttagacaagg
     9901 tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc catggaacgc tatattacgc
     9961 caggatcact aatgatcgtc ggcaaccggg cggaagtcca aaaattagca ttggaagatg
    10021 gcgccgcagt attgatcaca ggaggattca atacgactgc tgagattgca aagctagctg
    10081 atgatcttga aatgccgatc ttacgcacct cttatgatac atttacagtc gcaaccatga
    10141 tcaaccgagc gttaagcgac caattgatca aaaaagatat catgcttgtc agtgatatct
    10201 atatgccagc tgaaaagact cattatttgc agcagacaga tacgatttct gattatcagc
    10261 gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt caaccgccat catcgtttga
    10321 tggggattgt tacggcaaaa gatgttttag ggaaatcgcc aaaccagatc atcgatcggg
    10381 tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag tattgcctct gttagccatc
    10441 aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc cgatgacttg acattggaag
    10501 gcttgattac ccggcaagat gtaatgaaag cgatgcagct tgtccagcga caaccgcaga
    10561 ttgcagatac gatttcggat cagatatctg gaaatatcca tacgattgat actgaccgtg
    10621 aaggaaatcg tttggaaaac cctaagttca aattcgtagt agcaccacaa atggtcaatg
    10681 gcgttggaac catttctttt ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa
    10741 caatggtgat gaaccaaaaa cgaaatatct tgattgaaca agtgaaccta cattatttgc
    10801 ggctgatcca attggaaagc gaattagaca ttcgtccacg tgtattggaa atcggcagac
    10861 gttccgctaa actggatatc gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg
    10921 ttgtttgtca agtaatggaa cgttcttaga aagcaggagt gaaatgacag tacagcaaga
    10981 aatagtagat acaataaaaa aatacgataa aatcattatc cacaggcatt tgcgccctga
    11041 tcctgatgca ttaggatcac aagtaggatt agccgaacta ttacgggaaa gtttcccaga
    11101 aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt cgctttttag cagaaatgca
    11161 agaagttgaa gatgtcttct accaaggagc gctagtcatc gtgactgata cagctaattc
    11221 acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca
    11281 tcccaatgat gacccttatg gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag
    11341 tgagatgatt gcagaatttg ctttgatgtt tcctgaatct ttaaagatga acgttaaggc
    11401 tgcacggtta ctttatgcag gaatcgtcgg agatacaggg cgctttttgt atccctccac
    11461 gacgtcccgt acattagaga ttgcttcgat attgagaaat tatccatttg atgcaagtgc
    11521 tttgaacaga gagattgaac aaatgccgat gaaagtcgct aaactttccg gttatcttta
    11581 tcaaaatatc caggtggatg aaaatggtgc aggaagaata atccttccgc aagaaattct
    11641 tgataaatat ggaatcgatg attcagagac agcttcgatc gtttcacttc ctggagtgat
    11701 tgacgaagtg ctggcttggg ggatttttgt tcagcagcct tcagggtatt accgcgtacg
    11761 tttacgttct aaggggccga tcatcaatga attggctaaa cgccatcatg gaggaggcca
    11821 tccattagca agtggtgcga atgcaaaaga tttagcagaa gtagaggaaa tctataaaga
    11881 gatccaagat atatgccaat cgtataagag cagaaaaaca gaatgatact tcgttcgaaa
    11941 acgtgccttt tgtagtaaaa atcacaaatg tatcttttca agatcttctt tttccgttac
    12001 aatagtgctc actaaagatt ttaaaggaga aagaagatga cattaccaaa ttgcccagaa
    12061 tgtggctcag agtacacata tgaagatcga ggcttgttga tttgcccaga atgcgggaac
    12121 gaatggaaca aagaagaaca agaaactgtc atagaagacg gattagtggt attagatgca
    12181 aatggaaacc gtctgttaga gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa
    12241 ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga acatccgttt agtagaaggc
    12301 gatcataata tcgattgcaa ggtagaagga tttggaccga tgaaactgaa atcggaattt
    12361 gtgaaaaaga attaaagaat attcaaggtc ttttcacatt cttttcctac tactaatgat
    12421 aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg gttatggaat gtatggtttt
    12481 ttagatccga catttctttt ggtcattctt ggattagtta tttccggaat tgcgtcagcg
    12541 tacgtcaata gtactttccg aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt
    12601 acacaagcag cacaatatat attacaaagt caacagataa ataatgtcgg tgttcaacaa
    12661 attgccggag atctcacaga taactacaat tcaggtaata agatgttgag tttgtctcaa
    12721 gcaactgcac agtctacttc agttgctgcg ataggtgtag cagctcatga atgcggacat
    12781 gcggtacaag atgcttcggg ttatgtccca ttgaagattc gtgcagcact tgttccagca
    12841 gctaactttg gttcgatgat ttcgttcccc ttgattatgg taggtgtatt gcttagctgg
    12901 aactctacgt taatcaatat cgggattttc gctttttctt tagcattact tttccaattg
    12961 gtgacattac cagtcgaatt taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc
    13021 ggactactga cggaagaaga agtaccgatg gcaagacacg tattatttgc agcagcactg
    13081 acttatgttg cagcagcgct ttcgacattc ctgcagctgt tgcgcttgat cttattgttc
    13141 ggcggcaacc gaagagatta aaataaagaa gttgtgaaat cagcgctttg atctgagcta
    13201 ataccgagag ttagctgatt gaataccatt tatttggaa
//
LOCUS       DASJPH010000079        12969 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJPH010000079 DASJPH010000000
VERSION     DASJPH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.079"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..1005
                     /locus_tag="U3995_002130"
     CDS             88..1005
                     /locus_tag="U3995_002130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1797433.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(1383..2294)
                     /locus_tag="U3995_002131"
     CDS             complement(1383..2294)
                     /locus_tag="U3995_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1797434.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(2695..3273)
                     /locus_tag="U3995_002132"
     CDS             complement(2695..3273)
                     /locus_tag="U3995_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1797435.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(3293..3961)
                     /locus_tag="U3995_002133"
     CDS             complement(3293..3961)
                     /locus_tag="U3995_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797436.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(3976..5046)
                     /locus_tag="U3995_002134"
     CDS             complement(3976..5046)
                     /locus_tag="U3995_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797437.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(5217..6581)
                     /locus_tag="U3995_002135"
     CDS             complement(5217..6581)
                     /locus_tag="U3995_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1797438.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(6578..8137)
                     /locus_tag="U3995_002136"
     CDS             complement(6578..8137)
                     /locus_tag="U3995_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797439.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(8193..10673)
                     /locus_tag="U3995_002137"
     CDS             complement(8193..10673)
                     /locus_tag="U3995_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797440.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(10657..10992)
                     /locus_tag="U3995_002138"
     CDS             complement(10657..10992)
                     /locus_tag="U3995_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797441.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(11006..12040)
                     /locus_tag="U3995_002139"
     CDS             complement(11006..12040)
                     /locus_tag="U3995_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1797442.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(12130..12810)
                     /locus_tag="U3995_002140"
     CDS             complement(12130..12810)
                     /locus_tag="U3995_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1797443.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         3795 a         2630 c         2269 g         4275 t
ORIGIN      
        1 aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa gtcgtgagtt gcggtatagt
       61 tttatgtata aaaacgagga gaattttatg agcaacgtca ttatcaaagc aacaaacgac
      121 caacttagtc aaatgaaagc atactatcgc tcttatttgt taaataaaca gattccttat
      181 actagctttg ctgcaaaaaa agacgggacg accattaccg cttatacatc cggaaaagtg
      241 atgtttcaag gtacgaatgc agcaatagaa gctgataaat ggggggaggc acaggataaa
      301 caagccaagc cagcaaaaac agcctcttct ttgccaaaaa atatcagtca attatccgtt
      361 ttaggtagtg atgaggtcgg aaatggcagc tatttcggtc cgctaacagt atgtgctgct
      421 tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg gtgttagaga ttcaaaagag
      481 ttaaaagata gccaaatcat ccagcttgct gacatcttaa aaaaaaccat cccctataaa
      541 ctgcttgtct tatctccaga aaaatacaat gagatccaac caaaatacaa tgccgttcgg
      601 atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc taaaaaaaat cgagccagaa
      661 aaaccagaag cgattttgat cgatcagttt acgccggaag caaattttaa aaaatatgcg
      721 cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg ttactaaagg agaacaatat
      781 catttagctg ttgctgctgc ttctatcatc agtcgtgcca gctttttgga agagttagac
      841 aaagcatcag ctgaagctgg aatcaccctt ccgtcaggag ctggaacgaa atctgaccaa
      901 gtagcggcaa aactattgga gaaaggcggc atgcctatgc ttgaaaaata tgccaaactt
      961 cattttgcca atacagaaaa agctttgaag ctactaaaaa aataatggaa ctgtgacaaa
     1021 agtcatgtca cagtcccttg ttccgaataa acggtgttca atcatctgat cctcggtatt
     1081 agctcaacaa acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcaga
     1141 acaaaacgtt gatttcacaa cctttttctt aaacggtgtt ctcatgccaa ctcctcggtc
     1201 acaagtcaca atagtcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     1261 agagttgaac ggcaggctca cgacctcttc cttaaacggt gtttaaaagc ttgcatctgc
     1321 gataataagc ccggccaaac gaaaaatatg aggagctatt tttgtttggc tgggcttatt
     1381 tattactgaa aataagtggt caatgcttgt gtcaaacttg cagcaatctg ctgctgataa
     1441 ctgtctgtta ctaattcctt taaatcacta tcattattca tataacccat ctcgattaaa
     1501 atagaaggtt gaagattttc tcgaagaact tgatagttcc cagtggagat tcctcttgaa
     1561 tccagaggca gatcagtgaa ggagtcagca attgtctgtg ctaatgtttc ctcttgtcca
     1621 tagtaataat atgtcgtgat tccagttgct tcattcgcat attccgtaga gtctgcgtgt
     1681 aaactgataa agacatctgc ctttttttct tcactgtagt cgcatatatc tccaagctgt
     1741 actaattcat ctgtagtatg ggtcaaataa accgttgctc cagcatcctc tagcgccttt
     1801 tttactttta tggctgtact taatgttata tctttttctt ctgtttgttc atcattgctt
     1861 aatgccccag tatcatttcc gccatgccca ggatctaata caatcactgc atcagatagc
     1921 tcttccggtt ttgtttgtat attctttgtg actttggttc catcttcgag ggctaaatat
     1981 ccagtatagc catcatttgt cttgatctga taagcatttt ttgtttcaga aagaaactcc
     2041 gccagctctc cgccgtttat agttgcaagt ggggtagtta gcgagtcatc tgtatatagt
     2101 gtacggtcat tttttccgat tttgattaat gtctcttgtt ctgtatcatc aaatgattcc
     2161 tttgtctttg taaccgtact gttcgtactg ctgaccgttt gtccaactat ctttttttga
     2221 ccaaaaacat ctatttgaaa acagataata atgataatga ttgcaaatac aaaagctcca
     2281 attatttttt tcattcttga ctctcacctc gtagatccag actaagggcc aaaacttaaa
     2341 atcagcttaa gtaagcaaaa aaggggctgt aaaagatata accgtagaaa ataacattac
     2401 tgctaaaatg atttttttca tttttggcat atcctgaaat ctccttttga ctattcgtgg
     2461 tgattcatct acccaataaa tagtttcctt cattctacaa acaattaatt tcttcgttat
     2521 taaattaaaa cacttcctgg aacaccgaca taataaataa aaatataccc tgataatcat
     2581 gagagaataa caaatttgta tttatgaaaa actacctata taaagaagca agatataacg
     2641 atcttcgtca tatcttgctt cttcacctaa ctattttctc cctatataac agctttacgc
     2701 ttctcctaat acaccattca aatataaatc gatcaattgc gccacattta gcggttcttc
     2761 cacaggtctt ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat gccttgctgc
     2821 gatttctttt gaaatcccat ttcttagcac agattggttt gctgaaaaga tttcttcaaa
     2881 aggttcacga taatttttct tccatagaaa gaaaagatga tctcttgttt cctttgaaag
     2941 attgctttca agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa
     3001 ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct
     3061 caaagctttt ccaacatctt ttaagtggtt ttctaaaact gccgtatata gtttttcttt
     3121 atcaccgaaa taatgataga gattgggctg ggtgatccca actttttgtg cgatttctct
     3181 tgttgaagtg ccgagatatc ccttggacat aaacaagtca gtcgcttctt tcaaaatttt
     3241 ttgctgtgta tctttgttgt atcgatggac catttttcca cctgcttttt ggttattctt
     3301 tttctttcaa tacaccatct tccatcataa atattctatc gcatttgtca atcagccgtg
     3361 tatcgtgagt aaccatgatc gttgctttat ttttgtcttt tgattctttt gccaagatat
     3421 cgactacttc tcgtgccttt tctgaatcca aactagcagt tggttcatct gctagaataa
     3481 tggacggatc attgtacaat gcacgagcga ttgcgaccct ttgccgttct ccaccagata
     3541 gttcttctgg atacttgttg atcaacttat ccacgccaag ctgctcaaat aattcgtttg
     3601 cttctttttg acgatgttca tgtttaatct tatctactaa taataactgc ttttttactg
     3661 tcagaaaagg aaccagattg gaagcttgca aaataaagcc aatttcttta aaacgtagtt
     3721 taacgcgttc gctttctttt tgattactga attcttgatc attgataaat acagagccac
     3781 tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt acttttacct gaaccagaag
     3841 gaccgatgat tgcaacaaat tcccctttat cgatactcat ggttgtttct ttcaatgctt
     3901 taactacttg gtcgccatct tggaaacttt tttctacttc tttaaattct attgctttca
     3961 tgttcttcct ccttcttatc caattgcctt caacggatcg atttttacga ttgtccgaac
     4021 agaaaatagt gcgcctaaca cagctactac taacatcaac aagctaatga cgccaaaaaa
     4081 cagccaattg ctttggaatg gaaccgcatc aggtagaaca agggatgttc ccactgttcc
     4141 taatagacca agaagaatac caacaaatga taaaatgaat gtttgtgcca ctacagaacg
     4201 cgcaataaat cctcctgtga tcccttgtgc tttcattaca ccaaagatat taatcttttg
     4261 catcgtcagt acataaatga aaatcccgat cacaatagcc gcaataacga tcaaaaatcc
     4321 gatcataaat ccaaaagtca atacttgcgc attatatcca ggtaattcat tgatgaattt
     4381 agaaattttg atctgttcta aatcttcagg taaattactg attttacctc tgacaactat
     4441 tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg aagcggattt cttggtaagc
     4501 attgatggtt gtatagagaa ctggtgagac attgaatttg gcatgatccg taaagccagt
     4561 tagtttaaac gtcttgtcac taccagataa cttaactgta tctcctatgg ctagaccata
     4621 ttcttctttc aaactaatat cgcctactgc ctcatcatcg ttatcaaaag cttttccttc
     4681 aacaagattg ggcatgatga attgattttt atcgataccg aagaagctaa cattgatttt
     4741 tccttcttct gtactgtctt tactagtcac gactccaggt gtttgggcaa gatacgccac
     4801 tttcccacct tcgacttctt ctgctatctt cttagtaatc atggacatcc ctagatttga
     4861 atttgcatct ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt
     4921 atcttgagct aagccataag ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa
     4981 catcacccct gcaactaacg cataacgtaa ctttgaatgc ataatttcat ttaatgctaa
     5041 aaacatttaa ctcctcctcc tgaaataatc attttataca acaattttat cacttgataa
     5101 atttttggcg aatattatgc tcaaaaaatt cttccgctat ttcattatat gaatttttta
     5161 ttctcgattg attaatattt attatatccc cacacaataa aagaccgtga tatttctcac
     5221 agtcttttat ctactctatt gctatttgct ctcttagcta gtactttaaa agctaactca
     5281 ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc caaaatcctc aactgctagc
     5341 tgtggaatcg tagttttgtg tttcacgata tccgcaagca ggttcaaatc tgataataag
     5401 aattcttggc ttatcagctg tccgccaatt atttctaatg tatccggatg gtagatcagc
     5461 tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa atagatcaaa ttctcctgaa
     5521 cacgaaccgg tattttgttc caagaatggc gcttcatctt ctgtcagccc tacagttcct
     5581 aaaaagtagt ctataatgac tgctgctgtt atcttttgtc tgaggtcgta agcggttggc
     5641 tgaccactca aagttagcgc tactgctctt gccatatgta cagctcgagt aactaaaggt
     5701 aaaaagagat gccgtttatt tttgtcaaaa ctgactggga cgatatctcc aatcgcatag
     5761 atgtccggat cacttgtctg cagatattca ttgactatca cagtctcatc ctcatacaaa
     5821 gccacttttt cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa aatgaccata
     5881 tctgccttta ttgtttgaga cagaagttgt acggatacta ctttctcttc tgattttttc
     5941 caatcgatca agaattcatt tggataaaaa tttatttgct tagtttgcag ctgattttga
     6001 aaactgttga tcatatcctt atcgaaatat cttgataaaa cattcggcat ataatcaact
     6061 agatgaataa tgtatccttt atttctcaaa gcatctgcta attccaatcc aatgtaacca
     6121 gcaccgaaga tcactacttc ttttgcttct tttaaagcgg caagtacttg cttagattgc
     6181 actagatttt tataggtgta gacattttca gcttcaattt gtttatacaa ggtgacagga
     6241 gaggaacctg tcgctacgat taatttatca taggttatgg tctcgttttt tccttctttg
     6301 gatgcgagga tgagctgatg tttgtctgga tcaagttcag ttactctcca ttcggtgatc
     6361 agttttgccc cactttcttg aagagtccgc atgttatggg caacttcagc taaactacgt
     6421 atctttcctt ttaaataaag gttgattcca tttgaaacat agcctagtcc atctttatga
     6481 tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt tagggcagct
     6541 gtgatgcctt catgggaagc accaatgatc acgattttca tgttttattc atccttaaat
     6601 tcaatttttc taaataacat tcttttaaaa attggttcaa ataggggaaa tcataattat
     6661 attcgttgct agtgaataca aaaactttag cggacttctg tgacgggtac tctttcaatt
     6721 ggctagtcaa aatgatatca tactgctttt ctttctcgta ttcgactaaa tcatatttga
     6781 tcgtattcag caattgttcc tggatgattt ctttcacgat atccgccatt agcctttcat
     6841 aaggaagatc ataagcaatt tgcagacgta attctaactg cttgatcagc gtccgtaaaa
     6901 tagatgcata gcgagcaaat aattcaaagc gggcattttc ctcgtctact tcagtaaaat
     6961 caacaatagc aaatgtttgt ctgaccaatc tttcacataa aaccaggaat tgtttatctt
     7021 ctacttcagt taaccgctgt tttaatcctt cttggccaaa aatattcatc catcctctaa
     7081 aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt
     7141 ctggaaaaac ataaaaagag aaatattctt ctatcgattg tttaaacgtt cgattcaatt
     7201 gagctactac gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat
     7261 gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt tcattttgat
     7321 tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa
     7381 attgtgtcgt ttgaaacaat ccaacaggaa gattgctata atctggtttg gattttttcg
     7441 tacagcgcat tcgcttcttc ataatggaaa gccatatttc aaatttcaat cgtccttctt
     7501 ctgtaatagc aaaactaaac tctttttgca gcagatctaa aaaagtgttt cctgataaat
     7561 cactgtattc ttttttgttt tcaagatagg gacgtccaaa ccaaaataat tggaaataaa
     7621 aataactaat ttgactttct ggaccggtca gccgcccgct ttttattttc agatgaaact
     7681 catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa ctgatattaa
     7741 gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa agaagtatct
     7801 gatattttgt agaatagcgc aagtaacaat gataataaac atcgactgct gctatatttc
     7861 catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt ttgatatttt
     7921 ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca actgataagc
     7981 gggtaagtac gttattaacg gaaacctctc ctttttcctc taataaaagt tggaaaagtt
     8041 tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc actccttcaa
     8101 gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag gatctgtgac
     8161 agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc tgtcagttcc
     8221 catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct atttacctct
     8281 aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc tgttccatca
     8341 ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gctttgatcc agaatgattg
     8401 aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa agaagtcaat
     8461 gtacctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac atcaaaagaa
     8521 aaatcgcctt ttgattcata ataacttgtc ttcaacggtt tggggagatc gttaccaaat
     8581 gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag aattgtttta
     8641 gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt aatattgctg
     8701 gaaagtacag ctgcctttgt cagtatgctg acggattgta aagaagtatc tacagtattt
     8761 aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag atctgctggg
     8821 acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa ttttaaggaa
     8881 ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc ggttatcgca
     8941 tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca
     9001 gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact atctgcactc
     9061 acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc ttgttcagac
     9121 tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac caagcgataa
     9181 tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt atttacaccc
     9241 ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg
     9301 gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt tgctgaccaa
     9361 ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact atcggctgtt
     9421 aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt taaagcaaca
     9481 ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc tgtctgattc
     9541 aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac gttttttcct
     9601 gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa acgtccttca
     9661 gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg gttatttcca
     9721 tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc acttggtgag
     9781 atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc tcctggattc
     9841 aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa aatcgaattg
     9901 aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa aacggccttt
     9961 ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact cgtatcatat
    10021 ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg aagcgggata
    10081 tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa tatctgtcaa tagagtattt
    10141 gtatcccacg accccatccc aaaacgcaag gtatggcttc ctgctgataa taaacttccg
    10201 ctagtaacct tgatgggggc accggcaata tacaagtagg cgttgttcca gtcaattgct
    10261 tttaatacat cactgcttga tacatgttcc ggcaaattga tgatcgccac aaatgcaaat
    10321 ccttgtgcag tgatattcac caaatcaagt acatttgtta gtttcacatg gctttcccaa
    10381 tgaaaacgga ccgtgccacc tttccaagga tcatttgaac catcgttcaa ataagtagca
    10441 gtcaatttag gcaattggtt agccccagtt agttggacaa gcttcccgcc attgactgca
    10501 ctacgtccac catttggagg tacgatattt tcatacgtgt atggatcata agaaatggca
    10561 ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa
    10621 agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc
    10681 ctccttttcc tttcttctcc ataacaacaa ccacgcaaga agccataatt ctataccaat
    10741 gatcaaataa agaaaacgga tcttttgttc attcgtctcc gggaatacgc catgttgatt
    10801 agattcttta taaatcggta gctgtgtctg ctgttttgat gcgccattga ctgaaccact
    10861 tggatgcgga attggactta gatcataaaa tgtaatcgta cctgaacttt tggtatctgc
    10921 ttggactggc tcttgccagc tgaagaacag caaacttgca ataatccctc ccaataagat
    10981 cgatgttttc aacgaaatca cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga
    11041 cccacaaaaa cagcaaaatc aaccataaca ctagaactgc agcaaccaat cctgcataaa
    11101 gccatgggaa agattgttct tgtactgcaa aaacggaatt ttcatttact ttttgtgctt
    11161 tttctttttt gatcgtaaag ttttgtgtaa atcgccattc attactgtcg cctgattttg
    11221 ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc accagctaaa tcaagattca
    11281 agtgaaaatt cgaatttggt gcaactaaaa atgcattaga cgtattttca atataaagct
    11341 gattttcata aaagatcttg ctcgtaaata tcaaattgct gataatccta ggctgaggat
    11401 tttggaaacg aagctgaaca gttggctttg cgttgacctc gtccaaatct cctgataaaa
    11461 gtttcagatc cggttctatc gtctcttgac tttcttgtaa aacgactgcg agattataag
    11521 caatacgatt tttgatttgt gctccttctg tagcattttc tgtttcagct tcttctttag
    11581 gtgaaacatg aatacccgct aatacacgtc ccttaaagct ttttgctggg attttcactt
    11641 ctataggtac agttgtttct gcatgtgctg atacattgat ttcctttttc gcaatgagca
    11701 aaacgtcacg gacatcaaac ggcaatgtct catctaacgt tgggtttttt tgactataat
    11761 cgatcgttcc accatcactc gttaccgccg gattgacaga gacctcaaat gttcgttctt
    11821 catctgaaga attgctgata attacttcta gtgtctgatt ttgttccggt gccactaata
    11881 aatcaaaata accgattgct ttatctattt ggttctctgg aaataccggt ttgattccaa
    11941 agtctcctgc tgaatccttt gcttggctga ttgacggaaa aatgaaaaat agacttaaaa
    12001 tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat tgtccaccac atcctttcaa
    12061 caacaactgc ggtaaaaaat ccttcaccta tagtattttc accgcagttt gttgaattta
    12121 tttcatactc tatgctactg ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt
    12181 tccagctgct gctttcggca ctttcagtgt gatatttgct ggatcataaa cagaaagcca
    12241 tgttcccaga ccttgatctt ttgctgctgt aaatattggt gtcgctgcac ttgctttact
    12301 caacccagtt acagtttgac tagttggtcc agtggattcg ttatctgacg tacttttaac
    12361 agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc
    12421 tgaaaccgaa actgtcacgt tccacccttg tgcttggcca tctgcaccta cacctcgacg
    12481 atctgatact tggagataag gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt
    12541 tccgctttca atctcgtgtg taccaaatgg caattcggga actacatcca gtgttaaagg
    12601 acctgtctgg ccagttgctg gattttccgg atctgctggc tctactggtt ttgaaggatc
    12661 agtagggtta agaggctcca cggtatttgt tggttcagta aaggtaatat ctccttttga
    12721 agtcgcttga ccctcagcaa aagctggtgt tgccaagccg cctagaagaa gtgttgaaag
    12781 taataatgtc ccaatcatgt tctttttcat aataaatcac tcctttttct tagtgaccta
    12841 agtatagtag agacagtatg caaaaaatcc tcatgaattc atccgcttac ttgaaattat
    12901 gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt
    12961 ttttattaa
//
LOCUS       DASJPH010000080        12742 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJPH010000080 DASJPH010000000
VERSION     DASJPH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.080"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U3995_002141"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U3995_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1797444.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U3995_002142"
     CDS             complement(323..892)
                     /locus_tag="U3995_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1797445.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U3995_002143"
     CDS             1078..1980
                     /locus_tag="U3995_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1797446.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U3995_002144"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U3995_002144"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1797447.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U3995_002145"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U3995_002145"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1797448.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U3995_002146"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U3995_002146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1797449.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U3995_002147"
     CDS             8554..8739
                     /locus_tag="U3995_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1797450.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U3995_002148"
     CDS             complement(8801..9997)
                     /locus_tag="U3995_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1797451.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U3995_002149"
     CDS             complement(10167..11603)
                     /locus_tag="U3995_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1797452.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="U3995_002150"
     CDS             11896..12705
                     /locus_tag="U3995_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1797453.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4013 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aa
//
LOCUS       DASJPH010000081        12742 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJPH010000081 DASJPH010000000
VERSION     DASJPH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.081"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..544
                     /locus_tag="U3995_002151"
     CDS             101..544
                     /locus_tag="U3995_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1797454.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="U3995_002152"
     CDS             complement(550..1734)
                     /locus_tag="U3995_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1797455.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="U3995_002153"
     CDS             2002..2700
                     /locus_tag="U3995_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1797456.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="U3995_002154"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="U3995_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1797457.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="U3995_002155"
     CDS             complement(3510..4043)
                     /locus_tag="U3995_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797458.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="U3995_002156"
     CDS             complement(4340..6721)
                     /locus_tag="U3995_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1797459.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="U3995_002157"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="U3995_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1797460.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="U3995_002158"
     CDS             7422..7961
                     /locus_tag="U3995_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1797461.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="U3995_002159"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="U3995_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1797462.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="U3995_002160"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U3995_002160"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="U3995_002161"
     CDS             9068..10234
                     /locus_tag="U3995_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1797463.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="U3995_002162"
     CDS             10280..11776
                     /locus_tag="U3995_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1797464.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="U3995_002163"
     CDS             11857..12540
                     /locus_tag="U3995_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1797465.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DASJPH010000082        12670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJPH010000082 DASJPH010000000
VERSION     DASJPH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.082"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U3995_002164"
     CDS             6..305
                     /locus_tag="U3995_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797466.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U3995_002165"
     CDS             378..1739
                     /locus_tag="U3995_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1797467.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U3995_002166"
     CDS             1761..2021
                     /locus_tag="U3995_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797468.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U3995_002167"
     CDS             2035..2787
                     /locus_tag="U3995_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1797469.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U3995_002168"
     CDS             complement(2881..3486)
                     /locus_tag="U3995_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1797470.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U3995_002169"
     CDS             complement(3539..4513)
                     /locus_tag="U3995_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1797471.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U3995_002170"
     CDS             complement(4891..5610)
                     /locus_tag="U3995_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797472.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U3995_002171"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U3995_002171"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1797473.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U3995_002172"
     CDS             7161..8558
                     /locus_tag="U3995_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1797474.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U3995_002173"
     CDS             8639..9079
                     /locus_tag="U3995_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1797475.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U3995_002174"
     CDS             9227..9553
                     /locus_tag="U3995_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1797476.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U3995_002175"
     CDS             9554..10303
                     /locus_tag="U3995_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797477.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U3995_002176"
     CDS             complement(10383..10838)
                     /locus_tag="U3995_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1797478.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U3995_002177"
     CDS             complement(11265..11540)
                     /locus_tag="U3995_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797479.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U3995_002178"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U3995_002178"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1797480.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DASJPH010000083        12635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJPH010000083 DASJPH010000000
VERSION     DASJPH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.083"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..1111)
                     /locus_tag="U3995_002179"
     CDS             complement(146..1111)
                     /locus_tag="U3995_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1797481.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="U3995_002180"
     CDS             1345..2190
                     /locus_tag="U3995_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1797482.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="U3995_002181"
     CDS             2381..3568
                     /locus_tag="U3995_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1797483.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="U3995_002182"
     CDS             3574..3930
                     /locus_tag="U3995_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1797484.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="U3995_002183"
     CDS             3932..4270
                     /locus_tag="U3995_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1797485.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="U3995_002184"
     CDS             4665..5699
                     /locus_tag="U3995_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1797486.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="U3995_002185"
     CDS             5692..6378
                     /locus_tag="U3995_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797487.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="U3995_002186"
     CDS             6402..7250
                     /locus_tag="U3995_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1797488.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="U3995_002187"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="U3995_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1797489.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="U3995_002188"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="U3995_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1797490.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="U3995_002189"
     CDS             9516..10751
                     /locus_tag="U3995_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1797491.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="U3995_002190"
     CDS             10738..11208
                     /locus_tag="U3995_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1797492.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="U3995_002191"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="U3995_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1797493.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DASJPH010000084        12491 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJPH010000084 DASJPH010000000
VERSION     DASJPH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.084"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="U3995_002192"
     CDS             complement(324..893)
                     /locus_tag="U3995_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1797494.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U3995_002193"
     CDS             complement(967..2256)
                     /locus_tag="U3995_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797495.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="U3995_002194"
     CDS             2610..3458
                     /locus_tag="U3995_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN1797496.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="U3995_002195"
     CDS             3472..4545
                     /locus_tag="U3995_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1797497.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="U3995_002196"
     CDS             4535..6706
                     /locus_tag="U3995_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEN1797498.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="U3995_002197"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="U3995_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1797499.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="U3995_002198"
     CDS             complement(9009..11195)
                     /locus_tag="U3995_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1797500.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="U3995_002199"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="U3995_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1797501.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="U3995_002200"
     CDS             complement(11417..11857)
                     /locus_tag="U3995_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1797502.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="U3995_002201"
     CDS             complement(11931..12404)
                     /locus_tag="U3995_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN1797503.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3519 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa a
//
LOCUS       DASJPH010000085        12069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJPH010000085 DASJPH010000000
VERSION     DASJPH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.085"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..851
                     /locus_tag="U3995_002202"
     CDS             <1..851
                     /locus_tag="U3995_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1797504.1"
                     /translation="FVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDA
                     IDRALSDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNL
                     AKDFNEYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFR
                     EGLKECAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTI
                     QSQIPFVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQ
                     TRKSDT"
     gene            769..1821
                     /locus_tag="U3995_002203"
     CDS             769..1821
                     /locus_tag="U3995_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN1797505.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            1823..2140
                     /locus_tag="U3995_002204"
     CDS             1823..2140
                     /locus_tag="U3995_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797506.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            2267..2890
                     /locus_tag="U3995_002205"
     CDS             2267..2890
                     /locus_tag="U3995_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN1797507.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            2905..3795
                     /locus_tag="U3995_002206"
     CDS             2905..3795
                     /locus_tag="U3995_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN1797508.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            3818..4033
                     /locus_tag="U3995_002207"
     CDS             3818..4033
                     /locus_tag="U3995_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797509.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            4045..4386
                     /locus_tag="U3995_002208"
     CDS             4045..4386
                     /locus_tag="U3995_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797510.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            4386..4757
                     /locus_tag="U3995_002209"
     CDS             4386..4757
                     /locus_tag="U3995_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797511.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            4750..5145
                     /locus_tag="U3995_002210"
     CDS             4750..5145
                     /locus_tag="U3995_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1797512.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            5147..5524
                     /locus_tag="U3995_002211"
     CDS             5147..5524
                     /locus_tag="U3995_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797513.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            5525..6133
                     /locus_tag="U3995_002212"
     CDS             5525..6133
                     /locus_tag="U3995_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1797514.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            6133..6474
                     /locus_tag="U3995_002213"
     CDS             6133..6474
                     /locus_tag="U3995_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797515.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            6716..9505
                     /locus_tag="U3995_002214"
     CDS             6716..9505
                     /locus_tag="U3995_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN1797516.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            9495..10235
                     /locus_tag="U3995_002215"
     CDS             9495..10235
                     /locus_tag="U3995_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1797517.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            10232..>12069
                     /locus_tag="U3995_002216"
     CDS             10232..>12069
                     /locus_tag="U3995_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1797518.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         4448 a         1571 c         2399 g         3651 t
ORIGIN      
        1 attttgttac aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct ttggggacga
       61 ttaccttggc agaatttaaa aataatacga atcgttattg tgattttgaa gtagctgagg
      121 aaattggaga tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta
      181 agcttgcgta catgcttgtt actggcacaa caatgagcaa aggcactgct aaagaaatga
      241 tgaatcagct agggattatc aacttaaaag cccctactgc aaaagctgaa tatgtcacaa
      301 aaaatttagc aaaagatttc aatgaatatc atatggatct attgaaaaaa cagttttaca
      361 ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa atcaaacagt tctggagaag
      421 ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa tactaaagag caatatttta
      481 gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt catgacaaag
      541 tagaaattga gcctgaaaaa attattttta ctttctctag atctttgcca actgatttaa
      601 gttatttagc tgaagcttta cctaaacttg caccatacgt atccaaacga actattcaga
      661 gccaaattcc atttgtaaca gatgttgatt atgaaaatga aatgatggaa ttggaaagtg
      721 gaagtgctta tccagatagt gaatacaatt ttggcggagg tggcaatagt gacgatagaa
      781 acgaagtatt ggaccaaacg tcgagaactg gaggatcaag caaggctcaa acaagaaaat
      841 cagacactta aaaaattaac tagtgtattt cctgaagcac tgaaagagat acaagcaaaa
      901 ctattatcac aagctgactt acacaatatc acttatccag aaatgatgga gttttacagt
      961 acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa attataagtc attaaaaatg
     1021 tatgatgcaa aatacaaaga gtttatcgat gaattttttc caccatttga ctatgcaaaa
     1081 gtcaatcgct tattacaaat acgatcagat gtatttaaaa ttcttgcaga atatgcgatg
     1141 gatgcagatg tgaatcaata tttttctgat cgcttagagg aaattcttca aagaacatat
     1201 tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg atataccaaa ttatttacca
     1261 gaaaatgaaa ttaaacatta tttgaactat ccatggtcag gaaagacatt ttcaagaaga
     1321 ctttggggaa atatttcatc gcttgaacag aaactctcta atgctattgt aaaaagtgtt
     1381 gctagtggag aaggcgttat acacgcatta aacaccatga gattagactc agaaatttgt
     1441 gacatgttta agttagaaga atcaaagtat aacaaagcga tagaaaatct cgttcgaacg
     1501 gagtatgcaa aatttgcaca agatggtatt gaaaaatcat atttagaaac aggtattgag
     1561 gaatacaacg tattgactgc aaaagatgag agagtttgcc ggatttgtgg aggaaaggca
     1621 agtaagaatc cctataaact gaaagatgct gtcataggtg aaaatcgagc acctttccat
     1681 agtcgttgta gatgtacgga tgttcctaat ttaccaaaat taggaaagga tattgatgaa
     1741 gaatatgacc gtttatttgg cgatttatta gatgagtttg cacatgattc ttttggaatt
     1801 aatttgaaac ggaggaagta gaatgaaaga tttttttgaa gcagtactaa caattaatgt
     1861 aaatgctgat attgcagaag cctacaaaac agctattgag tctgagaacc atcctaatgg
     1921 cttgagagac cattggaatg gcaattatgc ctacgtggtt attggcgatc aaactgttaa
     1981 ttatcaagat aatactccag ttgataagaa taccgttaat ttaacgattc aattattatc
     2041 tcattcatta ccaaatttaa aagaaacagt tgattggtat gaaaagatgg gatgtattgt
     2101 tgttagaact gactacaaag aaggaaagtc tagtaattag gcttttttat tttgtccgaa
     2161 atgacgttaa actagcgcaa tactgggctt ggttgaatgg tggggcgcaa ctattaaaac
     2221 tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca
     2281 caaatcatta atgccaatga atttgcaata ctttgccgaa ggtgacgatc aaaagttttc
     2341 ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc
     2401 acaatctcaa agtgttgccg acaaacaatt ggaagcttgg aaacaaaata acttagataa
     2461 aattaaacag gataccatca aggaatatga ggaatctaag aaaaacaagt cacctgagca
     2521 aattcaactg gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag
     2581 tgataataaa gcatttgttg cagaacaaat tgctggatta gaactagatg gagagttaaa
     2641 agagtctatt tctcaattta tgctaaataa tcttgttagt tcggatacag atttcactaa
     2701 gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt aatgagaaac atgcagatgc
     2761 attaaaagaa ctacaaatga agtctgcatt tggtggaact caacaatcga ataaccaggt
     2821 tcagcagaac aatgaaacat taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca
     2881 atttaactag gagagtgaaa aattatgaaa aaaacatctt taaataattt agagtatttg
     2941 gatatttcac cagcgattaa tgctatgcaa gtaccaaata cacctttttt aagctattta
     3001 cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat
     3061 atcaacaacg atgattcttc tgaaaaactt gagggcggag aatatccaga tgctgaatca
     3121 ggtcgaactt ggtttaacaa ctatactgaa atttttagaa aatcaacctc tgtatctggt
     3181 acattagatg ctattaatgt gaatggagtc ggaaatgaat taactaatca agtagcacta
     3241 cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga ttactggtgt aaaagctgat
     3301 gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga acttgatcaa ttcagcaaat
     3361 aaggcagaaa cagccactgc gggtgcagta acaagaaaag atatcgatac tttatttaaa
     3421 ttgatgtatg aaaaaggtta tatgggagaa aaactatgct tgatttctcc agatatgcag
     3481 gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga
     3541 gtaacttttg gattgcaaat tggaaatatc gtgtctaatt acggtacagg tattgctcta
     3601 ctagaaccat cattgccaaa aggaacaatt gccgcaatcg atactaatta tgtgaaacta
     3661 cgtccattac gtgaatggag agcagaagaa cttgcaaaaa caacggattc tagacgtatc
     3721 ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa actctggggc aattttaaat
     3781 ttaaagtctg aataaaaggg agttagtact cccttttttg ataggaggaa ttacagtggt
     3841 aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat agagtaggta aaactaaaaa
     3901 ttttgttggg tttgttcatc ctaaaactcg tagatttatc acagcagatt caaataacga
     3961 atttatcatt tctatagatg ataaagaagc aattgcaatt ttggaagatg caattgatgt
     4021 taatgaaatt taggaagtga tctgatggat gaatcgctaa aaacggaaat cattgagtct
     4081 acaaaagaag attttccaga tttgagtgaa gaacgcataa ctaatttatt agaaataatt
     4141 ttgctagaaa ttgaatcata caacacttgt aaaaatgata tttcatggga aaagttaaaa
     4201 agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa aacagtatct
     4261 tcagttagac gtggcgatac aacgattagt tatgcttcaa caacaaatga tgttagtgag
     4321 ttacttctag gctatggtga tttgatacga agagttattg gttgtggagg attggagttt
     4381 ttttaatgaa tgaagcagat attttagaaa ctacttatga agatagttgt attattgaaa
     4441 gactgacgga cattgaagat tctaatacaa atattactat tcaagattac aaaaaagtat
     4501 atgataatcc tatttcttgc gctctttcac aaggtcaaat cgatggacta gcagtcatag
     4561 aagatggaga gatggtaaat gtttcaactg acacatataa attatttgtt catcctaaga
     4621 ttaaactaaa aaaaggagat cgaataacaa taactcaaaa ggccagtggc ttaatttttt
     4681 ctctatttgc tactaagcct ttttactatc ctagtcattg tgaagtgaat ttgataggaa
     4741 gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag aaaagatcat tgagaatttt
     4801 aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat
     4861 aaagtcgtta gtttagctaa accattaact cctgtaaaat caggtaattt aaggcgagga
     4921 tatcgtgtag taaaagctag aaaactatca agcggtcgaa ttgttggaga agttataaac
     4981 aatgaacatt attttaaata tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc
     5041 aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta tattcctcga
     5101 agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat
     5161 cattgctgca atcagtagca aattaaaaga aatctatcct gatgggacaa tatatcttga
     5221 ttcagttatg cagtcaacta aagattttta tttcgtttta tcagtaatgg aatctggaac
     5281 tgaaaatgta ggaattgata ttcaaaatgt ttctttctta attgatattg cattgattga
     5341 taataaccct aatagaaatt taattaatga attagtctca cgttgtggga ctttttttaa
     5401 tacgattaca atcgatgagc aaatattatt tccaaaagcc tatttacctg atgaagcaga
     5461 tggtgttcaa catattcgtt ttacattaga atttccacaa tatattgaat ggagtgaaag
     5521 ataaatggga gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt
     5581 tcctttggaa gatgagacag ggaattttcc ggtatatggt actgcgttgc caatgggaac
     5641 agcagtaagt atcaaaccaa cagctaatta tgaaacaaca caagattatg gggattcagt
     5701 ggttcaagat caatttacag cttttggtgg tgcagaagtt gaactggaag caaatggata
     5761 tactcataaa gttttatcta caattactgg agggaaaatt gttaaaggtg gtgccttgcg
     5821 ttctggagaa gatattgcgc aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg
     5881 gaaataccgt tatacggtct tttataaagg acagtttgct ctagattcag atgaaacatc
     5941 aacgattgaa ggaagtaaag taagttttac tcatccaact tggaagggtt catttgtaga
     6001 tgtaccagga cttggatata tgtattcagt tgatgaagat gatgaaggcg tggataaagc
     6061 aatgatcgga aattggttta caaaagtagc aattccaatt gaagaagcag aactttcagg
     6121 aggtacagaa taatgtcaaa atatcaaaca acgattaaat taacgaaaaa agacgaagaa
     6181 ggtaaatatg agcaagtaca atttaaatct gctgaatttt taccaggaac agttgtagaa
     6241 gatgctgcag gagttatgga agaaatgcaa acagcgactg ataagcaatc agttaaaaaa
     6301 gctttaagtc gtgcttattc atttattgca gatactttat ttgagggaca atttactggt
     6361 gaagattatt gcaaaggaat tgatgctcgt gagattgcct cattgacagg aaaattattg
     6421 aagtctgtta ctgcaggttt tgatgaaact tatacagaga cgaaaaaaaa gtaagtgagg
     6481 cgctcaagtc accttcattt aagtattcga ttacttaccg agaattagat ataaagacgc
     6541 aattactcga agcaggttgg acgttaccag aaattgaacg tactgacttg gatgagttaa
     6601 tgcgtctttt tgcttttaga gatgcggtaa aagagcatga agatgttgag tactatgata
     6661 atttcactca attttaggag gtgatacttt gaataacgat gacctaattc tgaagatgat
     6721 actagatgaa tctggtttta ctgccggtat gaataatgct gtcaaaaagt tgaactcttt
     6781 tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt tcattaggta gtatttggaa
     6841 aatatttgct ggaagttttc tagctagcgg agtaactaga atcgtaggag ctggctttga
     6901 tctaatcaag ggttccataa gtggagcggt tgatcgagta gatacgatga ataacgctct
     6961 acgaaatttc caaaatatgg gatttagtaa ttcagaaatt atgaagaata tcggaaagaa
     7021 tgggctttta tctcaaggta ttcaaggact tcctactgct ttgaatgatg cgataagtca
     7081 tgttcaactt cttgcttctt ctacaggtga tttaactcgt tcaactcaaa tatttaaagc
     7141 tttaaatgat ggaattcttg gtttcggtgg ttcaactgat caagttaatg aagctgttat
     7201 tcaattatct caaagtttct caaacggaaa agtagacgca caaacttgga actcaatgat
     7261 caacgctcaa cttggaccta ctctttctgc tattgctaaa aagatgggaa ttacaatggg
     7321 agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt
     7381 aatagaaatg gataccaaag gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga
     7441 ttctactaag ggaattaaaa cctctataca aaatgcaaaa acagctgtta cacgtggtgt
     7501 tggtgaggta atagaaggat taaataaagc attagtagat tcagatttag gtggatttaa
     7561 gggaattatt gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa
     7621 tattcctaca gcagtatcat ttttaagtaa tttatttaac gaaattcaaa aatttggttc
     7681 tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct accgcttttg gagcgttcat
     7741 gtttcaactt aaaggtatac cagcaataat aaaaagcttc aataatttta agaatgccat
     7801 aattggcgtt ggaaattcat taaagattat gggggcaata gctgctgcta atccatttgt
     7861 tttaattatt ggtgcagtcg ttggtgcaat agctgttttc ggttacttta tggcaaccaa
     7921 tgaagagctt agaaacaagg ttatatctat ttggaatgat gtgaaagatt ccgtaattgg
     7981 tgtattaaaa aatataaagg actggggaat tgatacttgg aattctgcta aagaaatggc
     8041 atcaaatgca gttgagggtg tcaaagatgc ttggtcagga acaaaggaat ggttttctaa
     8101 cacctggaaa ggtattaagg atggggccac aggtttattt gataaaacag tagagacttc
     8161 aagaaatgca gtcgatagtg taaaaaatgc atggtctaat atgaagaaat ggttttctga
     8221 tacttggcaa agtataaaag attcagccag agaaaaatgg aatggaatca aaggttctat
     8281 tatgtcagtt gctggtccat tgattacagg tataaagaat gcatttttac atgttacttt
     8341 ttatctagag accttatgga ataatttagt tgagattggt aaaaacgtct ttgagatttt
     8401 aaaaaatgtt atacttgcgc cggttttgtt tattacctcc atgatttctg gtggatggga
     8461 agaaacaaaa aataatatga ttgctgtttg gaataatatt aaagaaagtg ccataaatat
     8521 ttgggaatct ataaagaata tatttgtaag ttattttaca aatatttatt ttgctgcact
     8581 caatatttgg acagggttca agcttacatt gataaatatt tggaatgaag tggtaaatca
     8641 agctaaatcg atttggttaa acataaagta tttttttatt aatctttgga ttgatataaa
     8701 atattttgca attcagaaat ggattgaatt gaagttttct attattcaaa cttggatcga
     8761 tttaaaatat aatgccatta ctacttggaa taatattaaa cagtttttca aagatacttg
     8821 gaaaaatatt aaagatacgg catacaatac gtggatttct ataaaaaata ccatgattaa
     8881 tacatggaat aacattaagg actctttctg gaatattgtt actggaattg ttaattctgc
     8941 tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat gctattaatc gggtaaaaga
     9001 aatctttaat tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca tcattgatgg
     9061 acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca aaggtattgc
     9121 aggtaatatc aaaaattgga ttaaaggagc tttagatatt cattctccat caagatggat
     9181 gcgtgatatg gttggtaaaa atattgttca aggtattatc gttggtattg ataaagagca
     9241 aagtaagtta gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc aacctgtagt
     9301 aaatggaata agtcacctac caattacaca aacaaagcaa aatatatctg ctagtgaaac
     9361 aaaagaaatt catttacatt taaatgttta tggagattta cctgattcaa tggttcggaa
     9421 gattaccaca aaaattaaaa cagaattgac aagacaaatg aaacgagatg ctggtgcagt
     9481 aggaggaaca ttatatgcaa cttaagagag gacaattttt tattaatcaa cattattctt
     9541 ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt cgagtaattg
     9601 aattgagaga gcgagaagga aatgattcta tcattattga taaaggctat tacaaaaatg
     9661 tgactagaaa gattgagtgt tactataaag caccttcaat tgatatggtg caggagtggg
     9721 aagatcgaat cactgaatgg ttagatatgt cgtcttatag tgattttatt ctttactatg
     9781 atgaacaata tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa ggaacaagaa
     9841 aaacaggaaa tattgtccct tttgaattta cagtgagtat tcgaccattt aaggaaaatt
     9901 acaatggtag atttactatt cgacaaacag aaacttttga aatttataac ccagagaagt
     9961 attcttcaaa accgcttatt aaattgagtg gttctggaga tgcttctttt tatattaata
    10021 aggataaata tgatttgaaa tcgttggaca gagaattatt tatagattct aaattagaag
    10081 aggcttatcg aaaattagat ggtaatttag aacatcagga tcaggtcacc ttatttttag
    10141 attttccatt tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat attcattcat
    10201 ttgaaataat gcctaggtgg tggagaaaag tatgaaacca agaatatata gtcctactga
    10261 aacagatttc tcaacgaatg gcttaggaat tttaaaggat tgtacaagat gtggaatata
    10321 tgaagtagct aatggaaaat atgaattaga gttggattat cctttaggaa ctagatttga
    10381 tgaatatttt gaaaatgact atcaaataaa agcaaagcca aacgatcaag aagagtatca
    10441 tatctttttt attgatgata aagatataga tactttttta aatacagtaa ctatttatgc
    10501 tcagagtcgt acaaatcgac ttggaagacg ggtagtcact cttgtggaag tggactctaa
    10561 aactggtcaa gaagcgatat caattattga aactaaaatg gataaaaaat ctgacatacg
    10621 actttattct gatattacgg ctgtttctag cacaatcttt gaagcgagaa atgttttaaa
    10681 ttgtattgct ggtgaacaag gatcattgct tcaatattgg ggcggagaaa ttaaacgtga
    10741 accatttaag ctttctttgt taaagcgaag aggtcgcaat aatgttggaa cgattcggta
    10801 tggaaaagat atgtctggtt tgaaggtcaa attagattgg acaggtataa aaacaagaat
    10861 tattccatat gctgatcctc aaagtgaagt aggtacgact aatcgaattt atggttcgcc
    10921 agtagatagt gcctatatta ataactatcc tgatgtctat acagaacatg ttcagtttac
    10981 agaagaacaa ggagtaaaag acattaagag cttaaataaa atagctaaaa attactttaa
    11041 aacgatcaat cctggctgtg ataagcctaa agtttctatt acggttgaat ttgataagtt
    11101 gactgatagt gaagaagcga aagaatttgc gaagattaga aattatggtc tgtttgatac
    11161 atttaaaata tatcataaaa aatataatat ttatcttgaa tcaaaggtta gtggactaca
    11221 atacgattct ttatctgaaa aaactttgaa attagaagca ggagacattc aagttgcttt
    11281 ttatcagcaa caagctgtaa ctattcagga taaattaaaa gattatgcaa cgaataatta
    11341 tatgagtgat tttaatgatt atgtttcttc aatgattaca ggtcaaggca gtgcaggagg
    11401 atatgtagtt ttatggccaa aagaaaaacc ttccaacatc tttattatgg ataatccaga
    11461 tttagaaaag gcaagagaag tactaagaat gaataaaaat gggattgctt tttctaagaa
    11521 aggatggcgg gggcctttta attcagcttg gacattagat agtatattta atgccaattt
    11581 tatacaaact ggattaatca aagcagatat ctttcaaaat tcttttaaca agactggaga
    11641 tgtattgaaa ttagttaatg gactactcca aatttggaac aacaaaaaga aaataatgga
    11701 gttaactaaa aaaggaatgg aattttggaa ttctaagggt tcaattggaa cgattggaac
    11761 aactgattct gttggtaatc cttttcctgg ggcttctact ccaaccccta ttgaagataa
    11821 ttctttagtt attcgtacaa atggagacgg caaatatatt ttgatttctc ctaaagtagg
    11881 aaaaggatta gttttattag gaaatggtaa agcaatttat tttggagact tagatgtaca
    11941 aggaaaactt acagttaaag gaaaagaaat aattcctgga caaggtggtg gtccatcggg
    12001 aggtggagaa tctactggtg ggtatcctag tgaattaaaa acagatgcag aaaagagagc
    12061 ttggagaat
//
LOCUS       DASJPH010000086        12050 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJPH010000086 DASJPH010000000
VERSION     DASJPH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.086"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..297)
                     /locus_tag="U3995_002217"
     CDS             complement(133..297)
                     /locus_tag="U3995_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797519.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="U3995_002218"
     CDS             592..1920
                     /locus_tag="U3995_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1797520.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="U3995_002219"
     CDS             2122..2955
                     /locus_tag="U3995_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1797521.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="U3995_002220"
     CDS             2971..3708
                     /locus_tag="U3995_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1797522.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="U3995_002221"
     CDS             complement(3863..4831)
                     /locus_tag="U3995_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1797523.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="U3995_002222"
     CDS             5063..5896
                     /locus_tag="U3995_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1797524.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="U3995_002223"
     CDS             5930..6769
                     /locus_tag="U3995_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1797525.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="U3995_002224"
     CDS             6795..7355
                     /locus_tag="U3995_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1797526.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="U3995_002225"
     CDS             7554..9275
                     /locus_tag="U3995_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1797527.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="U3995_002226"
     CDS             9440..10582
                     /locus_tag="U3995_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1797528.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="U3995_002227"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="U3995_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1797529.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4109 a         2026 c         2500 g         3415 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
//
LOCUS       DASJPH010000087        11803 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJPH010000087 DASJPH010000000
VERSION     DASJPH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.087"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            606..812
                     /locus_tag="U3995_002228"
     CDS             606..812
                     /locus_tag="U3995_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1797530.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1014..2015)
                     /locus_tag="U3995_002229"
     CDS             complement(1014..2015)
                     /locus_tag="U3995_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1797531.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2020..3933)
                     /locus_tag="U3995_002230"
     CDS             complement(2020..3933)
                     /locus_tag="U3995_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1797532.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4101..4607)
                     /locus_tag="U3995_002231"
     CDS             complement(4101..4607)
                     /locus_tag="U3995_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1797533.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4767..5207
                     /locus_tag="U3995_002232"
     CDS             4767..5207
                     /locus_tag="U3995_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1797534.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5233..6390)
                     /locus_tag="U3995_002233"
     CDS             complement(5233..6390)
                     /locus_tag="U3995_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797535.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6393..6749)
                     /locus_tag="U3995_002234"
     CDS             complement(6393..6749)
                     /locus_tag="U3995_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797536.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U3995_002235"
     CDS             complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U3995_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1797537.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8217..9056)
                     /locus_tag="U3995_002236"
     CDS             complement(8217..9056)
                     /locus_tag="U3995_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1797538.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9241..10128
                     /locus_tag="U3995_002237"
     CDS             9241..10128
                     /locus_tag="U3995_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1797539.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10722..11417)
                     /locus_tag="U3995_002238"
     CDS             complement(10722..11417)
                     /locus_tag="U3995_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN1797540.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11401..11799)
                     /locus_tag="U3995_002239"
     CDS             complement(11401..11799)
                     /locus_tag="U3995_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1797541.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3514 a         2330 c         1779 g         4180 t
ORIGIN      
        1 agccactacc aaatatttgg tagtggcttt tttggttctg caagggtggt acagaacatc
       61 aatagtcggt gggttagaac gtatgtcatc gcatctttgg agaaggttgt attggtatta
      121 ttcgagttac ttgttagatg cgataacaaa gttagtatac agtagtaaaa aatttatctt
      181 ttcttttttt tattattttt gtttcttatt ttctaaaaaa ttaaaaaaaa ttttggtgaa
      241 ttaagtattt ccttgataac actggctttt ggcctcattt taggttatca tttgtaattt
      301 tatttcacag ttgatatgat tgttatgtaa aataaaaaag tttaggatac atcgaatttt
      361 tggggaaatt cttttggggg tatcctaaat cttttctatt atattcccat attttctatt
      421 attggtctag acgtatgatg gtaaactttg tacctatctg attgctgaat cattaattgc
      481 ttatcattag ccatctttgt ttcaagttcg ctagatgatc attttattca catcctaaca
      541 aacttgtcac atttatagat tcttaatcta tttatcatac aattataaaa aaggaggtgc
      601 tgcttatgaa agctatccat atttctttgc tttttttcgc tagtgcattg attttatttg
      661 ctattcatct ttgttcccag ccttatatca ataacaacgg aatattagtg gaaccagcat
      721 tcttttgctt accactcggc tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg
      781 ctttttttaa acgatcaaaa caacaaatct gacttataaa atttacgtaa aagagatcgt
      841 gacaataatc gtgtgatatt cccaaatttc gaataaaagg tgccccttgc catctctaac
      901 tcctcagtca caagttacaa tattcataaa ccatgaaaag cttgtttatt gaaattgctc
      961 cttgtgactt ggagctaagt gacagattac aacctctttt gcatcataag actttactcc
     1021 ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac
     1081 tgatattcat ggttcaaaac agcatttttc tccgcataat ctacttttgt gactttcacc
     1141 cccgctttct caaatacaga agttacttct tccatttgtt tcgtaaacgt atttgtattt
     1201 ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt
     1261 ttgaccttct ctgattgttc aaagtcctta ttatcaaagt aagcccatcc acatttttca
     1321 taaagaaagc tagagatttt atcacttact tgagtaagtg cagaaggatc caacagacca
     1381 gacaaagaaa taaacttcgt aatctgcttg ttctccaaag tcggatcaag atgatcagct
     1441 tttttaattt cttcattttg aagtgctaac aaatattctc cactaatttg tgcacctgcc
     1501 gaatttccac caatcgaaat attcgaccaa tctgcttgat cttttatctc ttttttagct
     1561 gcttgaatgg cttcgttcaa ttggataact ggtgtaggat gttttgcttc cggtgatcgt
     1621 tcataatcta tatttacgat gatttgctgc gtgtctgaac atagatattc caaataatca
     1681 cgacaatcat ttttatcccc tcctacatat gcgcctccat gcatccagaa aagaatcggt
     1741 aattttttct tttccacttt cggtgtgtag atatccatat tattattttt gtactttgaa
     1801 ggataggata tttctttttt tatatcgatt tgttcgattt tttgctggaa atcatctggt
     1861 cgggcatata cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag caagctaatc
     1921 ggctttggtg tgatttgatt aatcaaaaat acagtgacta aaataactac gatgataact
     1981 ggtactgcaa tcactattcg cttgaaccat ttcattcatt taccctctct ttcttcgcca
     2041 aatccatgtg ataatcgtgc taacagataa aaatccacct gaataaatga gaagcgaatg
     2101 tttcttttct ccagttttcg gcaacattct tttttttgca tctgtctttt tttgtacttt
     2161 cactgtcatc tgaatcggtt gcgtatcatc acttgattga tcctctcgag gttcattctt
     2221 tcccttattg tctgcattct ctgtcggatc ttgctttttt ggtggagcta ctgggatttt
     2281 tttgtaatag aaaataatag ttggtatcgt ctcattaaaa atcccttcag tcggtccttc
     2341 aacatttgct aattcgtatc cttcgaatgt cttttgctct gtcatataat tttctccata
     2401 agctccgtaa agctggattt cgtcctggtc tttgagtaga cttttattgt tttcatccac
     2461 atactttacg tttatctgat catggaacac tactttcaaa aagtccgtta catgtttcgt
     2521 gtaggcagca tggtcaactt cctgtgcctt tccatgccct gctccttcta ctaataaaag
     2581 atctttatac cctcctgata actcatataa tttttgcgcg ttagacacag gtactacatc
     2641 atctgctgtt ccatggataa tcaatttagg caatgaatct gacattccaa tcgtaggtag
     2701 agcagctttt gtatccaaat gcatctcctg atcaaaatag tattgtttca aaaggcccat
     2761 aaataaatct tcatgctctt gatccccaat gatgtctaac tgatttttta tggcatccgg
     2821 tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa
     2881 tccgcaattt tcaataattc ctttgacatt gacaggaata tctttcgcta atacagacat
     2941 catcgtagct gctcccatcg aaccaccata aagtaaaatc ttttgagatg gccgctctcg
     3001 aacttcttga tttatccagt aaaggacgtc atctgactca tattgtccat aggtcacata
     3061 atcgcctccg ctattaccag tcgctctgga atcaacaaat aataaattat acccggcctt
     3121 ataaaaatca ttgtattctt ctgtaacgat cccgttattc caattccctc gaaatccgcc
     3181 atggatcatg actgttttat ctgaaccttg atcgacataa tatgcatata catccatcat
     3241 tttgccatct tgtttgttta gtacagtttg atgaagttgt tttgtatttg gatagaagcc
     3301 tgtgttttct gtaggcgttg tcccatcatc tgtacctcca agcatcgaca tcaacacact
     3361 gttttttgca aaagcgatat cgtacaatag ataagtagca atcagatgtt tgataatctc
     3421 tttatctaca ccataatcat taacagcaag ctcagtgact aataactcta ttaattttga
     3481 cttgatgtct tctgttggtc cattaagtat ttctgtcatc tgtttaccga agtaattcaa
     3541 atattcttta ccattgaaat tttcaccttt accgatatca tcattttctg tcccattctc
     3601 tttgtcactt actttagcag aagaggattc gtcgtttgac atttttgtag ctgttgcttc
     3661 ttctgtttgt tggttatctg tggtagcggt cgtttcttga tctgtgctag taacagatgt
     3721 ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc
     3781 agcatcagta gtttctgtgg tttctacctc ccgagattcc gattcggaag aaatgctctc
     3841 tccatatact gtccattgcg ggacgcttgt acttacagca aataagatga ccaacgctcc
     3901 ctttaatact tgttttgttc tcaagctatt cactcctttt tatgtttcta ggagaattat
     3961 atgtaaacgc aaacattttt tatacaaaaa ataaatggtt tatcccataa aaatagaaca
     4021 ggattatttt ctcattttac gtaataaagc agaatggata aaccagtatc cattctgctt
     4081 tttttaaacg aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt
     4141 ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag gattatcata agttgtcgtt
     4201 gctcctctac acatcataag ctggtatcct aatccatgag caaacttcac agtcgtatct
     4261 atacaatact ctgcttgtgc cccacataat tcgatcgaat gacagtccaa ttcagttaat
     4321 aaaacactca aattagtctt ataaaatgaa ttcgcatggg tttttctcac aaaataactg
     4381 tgctcaggaa tagataattc cgacaatagc tcccatggtt ttgtttgtgg cactaattga
     4441 gcatcttcgt gttgaacgaa aataactggt ttcccaatcg tttcgtatgt tttgattctt
     4501 ttattcacta atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc
     4561 cctttttgta aatcgatcac tactattgcg tcggctgtgt ttgacatact tgtgccttct
     4621 ttcttttttc ttgatttaat agtatttctt atctagctag ttccattata cgtgacaaca
     4681 ttttcttatt ttacacctct aaatggcact tttcgactat ttcttcatgt tattatttca
     4741 taaatactta gctattggag gaatgtatga tgattaaatt gtatacaggt tctggctgct
     4801 cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga tataccgttt atcgaacaga
     4861 acttaattcg tcaaccacta accaaaaatg aatttatcca cttactaaaa ttgacaaaca
     4921 acggcgtgac cgaaatgctc tctactcatt ctattagtta taaaaatctt gttcacagaa
     4981 tagatgaact cagtttgagt gaactatata ttgagattcg tacatcccct tctttattga
     5041 aaaaaccgat tatctacgac caatctgcaa aacttttgac tggatttagc gaagataaaa
     5101 tcaggatgtt cctttctaaa actaaaagaa agcaagagct caacgaactc ttgctttctg
     5161 gtcatcttgt cgattttaaa gaaaataatc tttttgcaat gtcttaattt tggcataaac
     5221 aatctgcttt tcttaattaa cgaaatccaa tttttccaat atggatctat ttttctcatt
     5281 cctgattttt atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt
     5341 ccagtaaaat atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct
     5401 aatgacaata tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat
     5461 agataatatc cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata
     5521 gaatatgaca ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa
     5581 aatcaaccgt ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc
     5641 actaaaaaac tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc
     5701 ttctgctgat tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa
     5761 atggatcatt gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt
     5821 gaagtttttt tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac
     5881 tttatctgga gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa
     5941 ataaaggtaa gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa
     6001 aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt
     6061 gttcatctct tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat
     6121 tcttagtagg ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc
     6181 aaatttttct aatagttgac gtttattttt tatacttgat aatatatcag aatgattttc
     6241 gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc
     6301 tatcggatta ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag
     6361 ttctcttaat tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct
     6421 aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct
     6481 aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc
     6541 ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct
     6601 tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc
     6661 accacatata atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt
     6721 tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga
     6781 cttattccga cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat
     6841 actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct
     6901 gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact
     6961 tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc
     7021 aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag
     7081 cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt
     7141 ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga
     7201 tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct
     7261 gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact
     7321 tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct
     7381 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta
     7441 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     7501 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     7561 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     7621 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     7681 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     7741 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     7801 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     7861 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     7921 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     7981 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     8041 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     8101 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     8161 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     8221 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     8281 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     8341 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     8401 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     8461 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     8521 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     8581 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     8641 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     8701 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     8761 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     8821 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     8881 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     8941 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     9001 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     9061 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     9121 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     9181 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     9241 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     9301 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     9361 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     9421 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     9481 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     9541 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     9601 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     9661 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     9721 aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga
     9781 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     9841 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     9901 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     9961 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
    10021 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
    10081 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
    10141 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
    10201 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
    10261 ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga
    10321 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
    10381 ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa
    10441 aatatccctt ctggacttgt tcttcttatt ctgttatact agagaaacac gaaagatatt
    10501 tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga
    10561 gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt
    10621 tagtaatagt cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga
    10681 gttttcgaga aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc
    10741 ttttttagtt caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc
    10801 ctcactgctt tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac
    10861 gttatcgcat gagctccaga tcgaatggct gtagtgactt gttcacaatt tttgaaacta
    10921 gctgctaata tttttgttgg tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc
    10981 actttcatag catcaatgcc ttgattttgc atcctgttat aataaggtgc aatataatct
    11041 gctccattat taatagctaa atacccttgt aattctgtgt aaatagctgt accggtaatc
    11101 aaataacctt cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt caccggcacc
    11161 ttgataaacg tatcttttcc cagttcttct atgataactc gagcatcctc tatcatttct
    11221 tcttccgttc ttcctattgt ctgaatatgg ataggaatct gtccgattat ttttttgatt
    11281 tctttcatat gcgagaagaa gtttgacgct ccttcttttt tcagaattgt aggatttgtt
    11341 gttacgcctg ttaaattcaa aaactgttga caagaataaa tactttttgt atttgctgta
    11401 tcaagcatga tttccatata gctttccccc attctgatcc tcttgatcga ttttaatgat
    11461 tgtccccttt cttaaatgcg gcatttcctt atcttctaca tacattgttc ctggaagttc
    11521 cggctcatga gatccatcaa attttatcgt gatatgtcct aattcattca aattagtttg
    11581 gacaacatct cctactgctg tgattacata catttgttta tcaaaacaga gcttcatatc
    11641 acaagttatt tcttttctta cctgattcaa ttcaatctta taacaataat ctgttaatgt
    11701 ttccggtgct tcatctccaa ataaaatcat tattttttct ttcctgaata cttctgcttc
    11761 tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc tct
//
LOCUS       DASJPH010000088        11795 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJPH010000088 DASJPH010000000
VERSION     DASJPH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.088"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(155..2230)
                     /locus_tag="U3995_002240"
     CDS             complement(155..2230)
                     /locus_tag="U3995_002240"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1797542.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2232..3149)
                     /gene="glyQ"
                     /locus_tag="U3995_002241"
     CDS             complement(2232..3149)
                     /gene="glyQ"
                     /locus_tag="U3995_002241"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1797543.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3535..4353)
                     /gene="recO"
                     /locus_tag="U3995_002242"
     CDS             complement(3535..4353)
                     /gene="recO"
                     /locus_tag="U3995_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1797544.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4496..5395)
                     /gene="era"
                     /locus_tag="U3995_002243"
     CDS             complement(4496..5395)
                     /gene="era"
                     /locus_tag="U3995_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1797545.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5410..5805)
                     /locus_tag="U3995_002244"
     CDS             complement(5410..5805)
                     /locus_tag="U3995_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1797546.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5789..6265)
                     /gene="ybeY"
                     /locus_tag="U3995_002245"
     CDS             complement(5789..6265)
                     /gene="ybeY"
                     /locus_tag="U3995_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1797547.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6294..8486)
                     /locus_tag="U3995_002246"
     CDS             complement(6294..8486)
                     /locus_tag="U3995_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1797548.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8510..9481)
                     /locus_tag="U3995_002247"
     CDS             complement(8510..9481)
                     /locus_tag="U3995_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1797549.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10186..11565
                     /gene="brnQ"
                     /locus_tag="U3995_002248"
     CDS             10186..11565
                     /gene="brnQ"
                     /locus_tag="U3995_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1797550.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3202 a         2517 c         2157 g         3919 t
ORIGIN      
        1 ttttatttcc tcacttttca tgagcgtttt gaaaattcga acaaagaata aaataacaaa
       61 aattattaaa atagaattaa taacagctat ttataacaaa aaaaacagag acacagcacg
      121 tttcgtgcat gctctctgtt tttcaaacaa aagactattt tgtgatgatt tgatccaagc
      181 ttgctaatga caaagccatg gcagctattt gacttagttg tgttagtcgg ttcttacgta
      241 ctgctggttc atcagccatg accatcgttt gatcaaaata attttcgatc agtggacgaa
      301 gagcgacaag ctggtcgtag ttttcgtgga tcgtttggtc agcaaaactt gctttcattg
      361 cttctactgc tttgtgtaat tctttctctg cgtcgttttc gaacagctca gggtctactt
      421 ctgattgttt ttcaagctgc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
      481 ttgaaggttt gaagtcttca tctgcaagat gttgtttgaa caattgtgct gctgcaaata
      541 cttttgtcag atctttttgt tccgcattca aaacagcatc gatgacatca tgacgaatat
      601 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcact tgttgttgcc
      661 cttctgataa accaatgccg aaacgatccg tatctttact gataacatcg ctaatcgttt
      721 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga acgacaccgt
      781 atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc ccaactgcaa
      841 agaaagaaaa gatgctatct aatttatctg caatcgctaa aacagaacct actgtagact
      901 gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct gtcgctacag
      961 caggtttctc gcctttcaga agagcatatt tttcgcccat gatcccttgt agttcaggaa
     1021 attcccctac catgttggtt actagatcaa atttatagat ttcagcagct cgttgcaaat
     1081 ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca atgacttgta
     1141 cccgttgcat tttctcatag atactaccga ttttttcatg gaaagtaaca gtttttagtt
     1201 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
     1261 ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac atcttccaat tttacccgat
     1321 caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcattc cgtacctcga
     1381 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gaaagatatt
     1441 tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta
     1501 gtaaatcttc gtctagatca ataacccagt gatttttttc tgctaattcg tttgcttgtg
     1561 taacgatcat ttcttgtctt ttttgcggag acgcaatgac gaattgttca ttcaattttt
     1621 cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga tgacctcgag
     1681 aggctctgcc tgttttgaca tccaagattt cgaatgggat tacttcgtca tctaacaatg
     1741 ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa tgcatcgtta
     1801 cagggaaagt caaacttttg atcacccctg ctaagcctgt caatacttct tttgcttcct
     1861 taccatgagt gtatttcgtt acatagacat attccacacc ttttaattct ctaaaagtga
     1921 tagcgtctgt cgtcagacct tgtccgcgga caaatccttc cgctgctttc gtccagtttc
     1981 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca ctgtccgctt
     2041 gtcgttctgg aatatttgtt actttgattg ctaaacggcg cggtgtggaa aatgtttcga
     2101 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt
     2161 tcatgcttgg tgtgacaaca tgggcaggca tttcttccag cccgatttca agtaataagt
     2221 cttttgccat cttattcagc ctccttcacg gtttctggat ttgctgtgtt tttattcaac
     2281 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
     2341 ttgcggatgc gagaaagata accggcacgc tctgttacgg atactgcacc gcgggcgtct
     2401 aataagttga atgtatggct acatttcaag atataatcat acgccggatg aacaagattt
     2461 tcatcgatac agcgtttagc ttctttttca aaacgatcaa agttgcttag tagcaagtct
     2521 tggtcgctta cttcaaaaga atatttggaa tgttcatatt ctggttgttt gaagatctcg
     2581 ccatatttca cgccatcagc ccattctaaa tcatagacac tttcaacttc ttggatataa
     2641 gaagcaagac gttctaatcc ataagtgatc tcagaagtta ctggtttaca aggcagtcca
     2701 ccaacttgct ggaagtacgt gaattgtgtg atttccattc cgtcaagcca tacttcccaa
     2761 cccacaccgg cacagcccat ggatgggttc tcccagttgt cttcaacaaa acggatgtcg
     2821 tgttctaatg gatcgattcc taaaaggcgc aagctttcta aataaagctc ttgaatattc
     2881 tcaggtgaag gtttcatcac cacttggaat tgatgatgct gatacaaacg gtttgggttt
     2941 tcaccataac gtccatcagc tggtcggcga gatggctcta cgtaagcagc attccatggt
     3001 tctggtccga ttgcccgtaa aaaggtatat gggctcatcg tacctgctcc tttttctgtg
     3061 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
     3121 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt taaagcgttg
     3181 ctgtcaaata agaatacagg aaagaaaacg acaaaaaatc cctatgtctg attacagaca
     3241 tagggacgaa taattcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
     3301 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggctcccact ctcccgaact
     3361 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
     3421 ttgtttctat aatagatcct atcactgaac gatctattta atctatggtt aataatagac
     3481 ggaactccct gtgtttgtca aatggttttg attttttgtt gtatgtttta tttactattc
     3541 ttctttttca ggtgtctctt tttctttttc agacatgtct ttctctgacg tctttaatac
     3601 gtgtgcccaa gacttcattt gattgatgaa ctttttgctt ttcaaatgga taccgacata
     3661 ttcatcatac agctggtcga tgagttcacg gattgcttgt ttggtttctt ctttcagatg
     3721 gattccacta attttatcat aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg
     3781 aggatctgca tgataacggt gatcgtccat cgaccaatgt ctttcgcata tcacaccacc
     3841 atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacgc agtgtgtcca
     3901 gattggtgca attccgaagc gttgcagtat ttggacttca aaaatattcg tgatgatttc
     3961 tgcatcgtct ccttggtcca tccgttgcag tgcttgttgc gtaaactgga acaaatgcgg
     4021 gtcgtactga tgatcttcga ttgccgcatc tactagattc aataaatatg tgccatagcc
     4081 gctcaaaaag atgtcttctt ggatgtgtcg aaacgcatgg acatctttga cactgcttaa
     4141 aaatgacaaa ccttcacttc gaaaattccc gatataaact gcttcagtaa aagggagcaa
     4201 agccggagct aagggctgat tttgccgatg gatctctttg atataaaaca tcattttgcc
     4261 agaagattct gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa
     4321 aatcaagcct tttgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg
     4381 aaaagcagat tctgccgatc cagatcattt atgttgcgat cttccgcagc ttttctcaac
     4441 acgcggattt ttattcagtt tatttctata cctaatttac aagaagctca gaatatcagt
     4501 attcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt
     4561 gaactttgac ccaaagttct agaaagactt tgtctcctaa aagattttcg atatcttttc
     4621 ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg atcccttttt
     4681 ggctatctct ttcgacaata atcgtagcct ggatatggat tttatcattc tcgtttcgct
     4741 tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga
     4801 ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat
     4861 ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatc aaacgttcga
     4921 cgttgtttcc ttctgtagca gagattggaa tcacttgtgc aaaaggcatc tgcgttgaat
     4981 aatcttcgat gattcccaac aactcgtctg gatggatttt gtcgatttta ttgataatca
     5041 gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccgaccgc
     5101 gtttttgatc agcactaatc ataaataaga ccgcgtccac ttctttcaac gcactgtaag
     5161 ctgcttctac cataaaatct cctaaacgat gctttggttt atggattcct ggtgtatcaa
     5221 taaaaatcaa ctgagcttct ggtgtagtgt atactccttg gattttattt ctagttgtct
     5281 gtgccttgtc actcatgatg gcaatttttt gacccacgat tcgattcaat aatgtggatt
     5341 tccctacatt tgggcggccg acgattgcga caaaccctga tttatgttct ggcatatatg
     5401 ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaac
     5461 gatgacggta aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt
     5521 cccaatcgga tgaaaatgaa aatccgtaaa catgtccacg acattttcaa aaacagtatt
     5581 gatgatctct actatccata cgagaaaaac agctaaaagc agccataacc attctatccg
     5641 atcaagttgg aaaatcacgc ccataaacaa agcgaggatt ccgaacgcaa catgtttcct
     5701 catgttgcgt tcttcctcaa aaacagtttt gactccttgg acggcaaatt ccacggacat
     5761 caggaaatgt ttgtttttct ctacgttttt atcttttaag tccataggca tctaagatct
     5821 ctttctgtaa accaaacatt tctttctcat cctctggtgt catatggtca tacccgttga
     5881 tatgcaagaa tccatgaacc gctaaaaagc caagttcccg atcatacgaa tggccgtaag
     5941 cttctgcttg ctctgcagca cgttcagtag agatcatgat gtctcccagt acacgaggca
     6001 atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccctct tcttccaagg
     6061 caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga
     6121 ttgctgcatt gtccatgaaa gtaacagaca tttctgtttc ttctggcaac tctaaatatg
     6181 atgctgcaaa ttgcagcaag tcgtcgattt ctttcatttt ctcttctgag acttggtttg
     6241 tttcatccat gaaggtaata tccattatac atcgcttctt tctattcttc ttgctattct
     6301 tcttgttctt cttttatctt ctcaacttcc gatttcattt taggatattt gatccgagaa
     6361 tggaaggtac tgcttaaacc gctgatcaat gatttttcta ctttacggat ttcttttaac
     6421 gtcaatccac tgtcgtccaa ttgtccatcg gaaatacgct cttcaataag attatggaca
     6481 aaactttcga ttttttcgct cgttggatgg tccattgccc gtactgccgc ttcacaactg
     6541 tcagctatat tgacgattcc tgcttcacgg ctttgaggtt ttggtccagg ataacggaat
     6601 tgctcttccg taacttccgg gttgcgttcc tttgccttaa cgtagaaata acgcatcagc
     6661 gtggttccat gatgctgtct acaaatatcg atcaccattt gcggcatatt ataatcttcc
     6721 aatattttgg caccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6781 aagaaattgt gcggattttc cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt
     6841 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagtaaaga acgtccaccg
     6901 atttctgcta cagcattagc acttagattg gctaccatca tgctatggtg atacgtacct
     6961 ggtgcttctt ccagcaactg tttcaataaa ggatgattgg gattgctgag ttcatttaat
     7021 acgatcacac tatcatcatt gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc
     7081 aagaatgaaa acagtgcacc tgtgaatccg cagactagcg ttaaccaagt cgttgcatcc
     7141 ccaaagctca ttccttggta gataatcaaa atgacatcca tcatcacagg aaaaataatg
     7201 acccacatag cagcggataa cccttgttct gagatccgtt tgcgtctgac gatcgttgcc
     7261 atgagaccag aaaatacata ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa
     7321 tcataaaaaa tgaaaagtgc ggatactgct tgaaaaatac cagccatgat ccctgctctt
     7381 cgattcaaga aaaagcttaa aacaagtgga gcaaaagccg cggggtaaaa cagcggaata
     7441 taagcagctt gttctgtttg gaagagttgg aaaaatttca ttagtacgac gcttactgac
     7501 attgccgtca cgtagaaaag tacgtatttt gttctttctc ctgcattttg gaattgaagt
     7561 gaattataaa tcagtacacc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
     7621 aaaatagaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
     7681 tcaatttgat ttccttcgcg aacaataatt tctccttgga agatcatcac tggttgtaca
     7741 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattaact
     7801 acgatgcctt gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa
     7861 tattgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
     7921 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taactgtgtg aatttccgtt
     7981 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
     8041 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
     8101 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgccattata agatttgttg
     8161 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
     8221 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt
     8281 ctttgttcgg tttcttccgt gttctcaatc gttttattcg cacggatact ctcttctgct
     8341 acttggcctt ctctgaaatc aatggccttt tgtttgacac tactaaatgt aacgccgcag
     8401 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt
     8461 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8521 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8581 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttaatgtt cgttctgcat
     8641 gaacaagacc acttgtcgtc ccacgaggca agtctatctg acttgtatcc ccattgacaa
     8701 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
     8761 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
     8821 aaggtgcgat ttcgatcacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
     8881 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
     8941 cgggtagaaa gcctaggttt tcccctgcct ccacagcggg ccgcgtcaaa atgatttttt
     9001 gcacttcgcc tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg
     9061 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct
     9121 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9181 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacaa
     9241 catctggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
     9301 aggcagccaa ggaaatctgg gattgctcac caatgatctg aatgatttct ccacgtgtat
     9361 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
     9421 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
     9481 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9541 ctatatagca aataagaaaa ggttgtaaaa gagtcgctta accatacgta ccaagaaacg
     9601 ttaaaaaata gccgtttcca ttcgctaaag gtctcgtttt gataaaaaga agctgagaat
     9661 gtaagttata tgaaagtgga gaacaaaata tttcgtttca ttcaacaagt attctctagg
     9721 tatttttcct gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc atttcaaaaa
     9781 aaacaggtgt attattacgt caacaaataa acatacagca aagcgtcgag gaaaacagta
     9841 ggctattcaa cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat
     9901 agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta
     9961 tcaggctagc ggataacttt atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga
    10021 aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt
    10081 ttattttaag gtggtggttt tcatggaatc tgatattagt ggcattacta attgctgcaa
    10141 cgtttatatc gtcaatcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa
    10201 acttattcat ggaaacaact attgatggtc agttcactta tcttcgggat gttcttcggg
    10261 tcaggtaatc taatctttcc tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt
    10321 gctggcgtag gatttgctat ctcaggtgct cttttcccat tactagctat cttagctgta
    10381 gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct
    10441 gccttgttct taatccttgt tcatttgacg atcggaccat ttttcggtac accacggaca
    10501 gctgctactg cttttgaaat ggctgctaaa ccatttttgc ctgaaaaata taattcatta
    10561 ggtatgtttt tattctctgc tcttttcttt ttacttgctt atttccttac tgtccatcca
    10621 acacggttgg tcaaatacgt cggaaagtat ctaaactcct tgtttcttgc attattagca
    10681 atcatttttg tcgtttcttt attccatcca atgggaaatc taaatcaatc tgctgcagat
    10741 acttatcaaa caaacgcttt aacaaatggt gtcctagaag gttataacac ggttgatgca
    10801 gtagctttgc ttgccttaag tgtcactttt gtccatgcag tccgtgacct tggtttcaaa
    10861 gataaaaaag tcacagaatt aacggcaaaa gctggtagcc tgagcgttgc gctagaagtc
    10921 atgatctatt ttggacttgt tcttctcggt gcgatgagtt tgaataagat gtctttatca
    10981 gaaaatggcg gaactgcact ttctcagatc actaattttt atttaggtaa cttcggcgct
    11041 agcttcttag gagtgatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt
    11101 tcttttgcgc aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg
    11161 ttaacgactt tcgtctcttt tgtcgttgca aatgctggtt tagacaatat catccaatgg
    11221 tctttaccag tattgatgct tttgtatcca ttgtcgcttg cgttgatctt gctttcctta
    11281 acagcaaaat ttttccaaaa gacaccattt gtctatcaag taacgatgtt gtttgctgct
    11341 gttcctgccg tattggatat gctggcaaat tcgcctgctt tagtgagtca acaaagagtt
    11401 gtagcaagca tgctcgaatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc
    11461 tggatggtcc caacgttact tggttacgct ggcagcctac tgttttatta cgcctatcgc
    11521 ctatcagggt ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa
    11581 tttaacataa gaaatattaa acaggaaaaa accagctaga tgaaagaatc ctattctttc
    11641 gtctagctat tttttagttc tcttttttac gcgcacaaaa aaactgcgga caatgattgt
    11701 ccgcagttta aatgggctct ataatatcta gtgttggtga cgccttctgt tgccaatact
    11761 ttttcttttt tctaataaac gcaaacagac acctt
//
LOCUS       DASJPH010000089        11567 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJPH010000089 DASJPH010000000
VERSION     DASJPH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.089"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..822)
                     /locus_tag="U3995_002249"
     CDS             complement(127..822)
                     /locus_tag="U3995_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1797551.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            976..1254
                     /locus_tag="U3995_002250"
     CDS             976..1254
                     /locus_tag="U3995_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797552.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1273..2481
                     /locus_tag="U3995_002251"
     CDS             1273..2481
                     /locus_tag="U3995_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1797553.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2629..2994)
                     /locus_tag="U3995_002252"
     CDS             complement(2629..2994)
                     /locus_tag="U3995_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1797554.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3141..3914)
                     /locus_tag="U3995_002253"
     CDS             complement(3141..3914)
                     /locus_tag="U3995_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1797555.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4088..4948)
                     /locus_tag="U3995_002254"
     CDS             complement(4088..4948)
                     /locus_tag="U3995_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1797556.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4948..6366)
                     /locus_tag="U3995_002255"
     CDS             complement(4948..6366)
                     /locus_tag="U3995_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1797557.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6423..6872)
                     /locus_tag="U3995_002256"
     CDS             complement(6423..6872)
                     /locus_tag="U3995_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797558.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7317..7580)
                     /locus_tag="U3995_002257"
     CDS             complement(7317..7580)
                     /locus_tag="U3995_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1797559.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7679..8974)
                     /gene="rho"
                     /locus_tag="U3995_002258"
     CDS             complement(7679..8974)
                     /gene="rho"
                     /locus_tag="U3995_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1797560.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8971..10242)
                     /locus_tag="U3995_002259"
     CDS             complement(8971..10242)
                     /locus_tag="U3995_002259"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1797561.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10407..11276)
                     /locus_tag="U3995_002260"
     CDS             complement(10407..11276)
                     /locus_tag="U3995_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1797562.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         3264 a         2434 c         1992 g         3877 t
ORIGIN      
        1 gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
       61 aactattttt ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
      121 tgttcatcaa aagataatat tgtcttctaa caagtccata ttcaaaataa cgatatgatg
      181 gtctttaacg gtgataatac catctttttt cagttgtttt aaaattctgt ttacactgct
      241 agcagttgag atgccacaaa atttggcgat ttcttcattc gtgatcgaaa aatcgatcag
      301 catcccttct tctgtccgca caccgaataa atcatataat tcgtaaagtt gcgtacaaac
      361 agctcccagc tttccgttta atagcatttg ctgcattttt ttcatcgaat aaagcagacg
      421 atagcggtaa tactctttta catagtgttg tagctttggg tcttggttga tattttgcca
      481 aaagcgcaca cgattgattc gataaaactc tgcatattct gattcaactc gaatattata
      541 cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga gagacgattt caagatcttt
      601 gatatagcga aggttaaatt ctctcccatc atttgaaata acacttgctt ttaccgttcc
      661 ctctttcaag atatacgtat gggaatcttc catcccttcg aatgtcaaat atttatgata
      721 tcctttgcgt ataatcggaa aatcattttt atccaaatat tcctttaaga ctctactatc
      781 caatgatgct cccccatcca aactttcatc taaaaagatc atacagtact ttaaaacttt
      841 ttaaagtcca aaggtatttt actaatgata ttaacatttg ttaagtacct ttttccatca
      901 cttatgcgat ttttttcctt ttttttattc gttatactgc agaagtaaaa ttttttaaga
      961 atagagggaa aaaacatgaa ttactggatg aacacaatca ttaatcgatt agaaacagca
     1021 tatcaaacaa gatttgatat gaaagcttct ttagtctttt taaatgatgc gtatcaaaac
     1081 tcgatcgaat tgatcaaagc agtggatgag aatccaacaa acgaatgcga agaattttta
     1141 aatctgttta tgtctacaag agatttattt attcgccaac ttgttgatcg ctatccatcc
     1201 aattatcacg atgtagaagt tcaaatacaa aaattaaaag catattcagc ttaaggggaa
     1261 tgtataacat acatggaaaa gaaattattt aataaatatt ttattggaat cacattgatc
     1321 aattttattg tctacctgat ttattatctg ttgatggtca tcatggcagt cattgcgcaa
     1381 gacaccttac acgccagctt gagccaagca ggttttgctt ctgggatcta tatcatcgga
     1441 acattgctgg cacgcttatt catggggaaa cagttagaac tatttggtcg gaagtttacc
     1501 ttaagaggcg gcgcgatctt ctatctcctt tcaacattag catatcttta tacgccaact
     1561 atcggggcat tgtatttagt tcgattcttg aatggattcg gctacggtac agtttctaca
     1621 gctacaaatg ctatcgttac tgcatatatc cctgcatcta aaaaagggga aggcatcaat
     1681 tattatggat taagtactag tttagctgcc gctgttgggc catttttagg aatgattttg
     1741 ctgaatatga ctgattttcg tttcattatc tggttttcaa ttgtactggt cttctttgtt
     1801 acgattgcct gccttgtctt tcctgtaaaa aatattccat tatctgaaga acaaaaaata
     1861 tctcttcgct catggaatat caatagtttt attgaaaaga aagcactttt cattacagga
     1921 attgcctttt tgatgggtct ttcttattca agtgttttat catttttatc ttcttataca
     1981 aaagtaatcc acttagttgc tgctgggtca tttttcttcg tcgtatatgc tttggtcatc
     2041 acatttacac gtccattgac tggaaggatt ttcgatgtca aaggagaaca atacgtgatg
     2101 tatcctagct atttattctt aacagcagga ttatttttac tgagtgtaac gacgaatagt
     2161 gtgacattgc ttatctctgg agcattagtc ggcctaggtt atggtacgtt tatgtcaaat
     2221 ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa tcagtattgc tttatctacc
     2281 tactttattg gtttagacct aggcttaggc gttggtcctt atttgatggg gatgctaaaa
     2341 tctcttactt catttagagg attgtatgtg attgccggag tcattccatt gatatgtaca
     2401 gcactctatc ttgttaatgg ccgaaaacat agtcatgaag cagagggcga attgattgaa
     2461 tcattgaaaa atgaaatttg atcttgatga atagataaaa aaaaccgttc agatctgaaa
     2521 ataatctgag cggttttttg agtaaaaata gtttatgatt attcttaatt aaacatctat
     2581 ttatttttaa agctttcatt tatcgataag aatagtttaa ctgttgattt attccatttt
     2641 ccgttcatct ggtacgatgc cttgacttag caaccacttg tggaagaacc attctgccac
     2701 accgatcaat agagcaggaa tcaatacttt catccaaata tcgactgtat cattcataaa
     2761 atatagatag agccacaaga taactgcagc tgaaccagca tctgcgatcg tcgctgcgat
     2821 gttccctact ttacgtaaga tcaacagatc ccctagcaca tagatcatga ctacaataat
     2881 taccccaaca atcgttgaat ccataaagct aacaccgtat aaacctgtca gcacgaccca
     2941 cagaatcgcc cagatcatga ttgccttcac taccaacgcc ttcacatgtt tcattttctg
     3001 cactcctttc tcctggataa aaaagtaaga ttatctaaca agataacctt ttcattatta
     3061 taccatcggc tgagagaagc gagcaaagat aaggtcctat ctaaaaatga ggaagccact
     3121 tagaacattc acgtaataat ttatccgtac agacattcga tatggtgttc ctttactttc
     3181 gcatactgtt cttttgtcag ttctttactt gtcaccagat aatcgatttc atgaaaagca
     3241 atggcttgat gcatgaattt atgtgcaatt ttttgctgtg taactaaaac ggcaagccgg
     3301 tctgattgtt ttctaaaagc ttttttgacc attgcctctc cttcatgagt ttctgttact
     3361 ccttggctga aatctagccc gcgacaactc attatcgcta aatccgcata atatgacaat
     3421 gcatcgttat aggcttttga accgttgatt gttgctcgtt tcggaacaat tgcccctccc
     3481 aatacagata cttttgccgt tgtatattca ctcaacatag cagctgtgac gatgccattc
     3541 gtaatgatct gcaaaccatc aaatcttgct aggtttctga ctaagtgtaa agaggttgta
     3601 ctggaatcca agaataagac catatcatct cgaatcaaag aagaagctgt atctgcaatc
     3661 ttacgttttt ctgtttcttc ttctaatttg gtgatcttca tcaggtaagg cacgtcaatt
     3721 tttcgattgt caataagcat ggcgccaccg cgaactctac ggatcaatcc acttttttcc
     3781 agaaaaacaa ggtcacgttt aatcgtggct tcgctataat ttactaactc gatcaaatcg
     3841 gtgactcttg caaattggtt gttttttaga taattcacaa tctcctgctg tctattctcc
     3901 acttcattca acataaaatc accccattta ctttatatag taacaggaaa aaagaggttg
     3961 tgacagaagt gattggcttc gagaaataag aagaaatttt tcgaaaaaac tacttctgaa
     4021 catcgtgtgt ggttaagagg gtatgacaag actattgtca taccctcttg accacacatt
     4081 tttattttta gtataaacta gccttgccta ctgtgttcaa aatttccatt ttatgtttga
     4141 ccacttcttg taccttttta ttagctgctg gataaattgc attcggctcg tacatcgatg
     4201 gattttcact caatatttct ctcaaagcat tgaaaaagac gcttttaaga tctgagctca
     4261 aattcacttt ggcaactccg tattttactg cttcgctgac ttctgcgtcc ggattacctg
     4321 aaccgccatg taaaacaaat ggaagatcga tttgttggtt caattctttc agtaagtcga
     4381 tatttaattt tggagttctg tttttcggat acaagccatg cgctgttcca attgctacag
     4441 caagtgtatc gattcctgtt tctttcacaa aaatcttcgc ttgatcagga tctgtataaa
     4501 tgattgtttc agcacctcct tcagcagaac cattattccc aatcgttcca agctcagcct
     4561 ccactgagac atttaccgca tgagctaacg caaccacttt tttcgtaatc tgtacattgt
     4621 cgtcgaatga tttcaaagac gcatcgatca tcacggaaga atagccgttt cgaatcgccc
     4681 gtaagacatc ttctatcgta ccgccatgat ccaagtggat aacgatcggc agtttacttg
     4741 tataagcata ggctcttact gccgcaacaa actcatcact taaataggcg atttcatcag
     4801 gatgaatttc taagattact ggtgcttcta atctctctgc ttcttcgatg attgctttca
     4861 tcatatcata gctgcaaata ttgaaggctg gtactgcaaa ttgattctct ttagccactt
     4921 tcaataactc tttcattgta tataacatta cgcttccacc tcatttgtta gattttcatt
     4981 tttcgtccac ttgtcttgtt ttttctcttt cactgttttt ttcaatagtc caagcataaa
     5041 ggctgtagtg actgtacctg atgcaattgc caagacatac ataggtaaat tgttcataac
     5101 atttggaatt agcatcaccc aaatccctcc atgaggtgcc attgaagtaa ctcctactgc
     5161 catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa ctaggtatca cgcggatcgg
     5221 gtcagatacc gcaaacggta tagcaccttc tgtaatatac gaagcgccta aaatccagca
     5281 tgattgacct gaagtccgtt cttctttggt aaatttatgt ttgaataata aagtggctaa
     5341 agcaatacct aggggaggtg tcattcctgc agccatacaa gcagcaatcg gtgcatttac
     5401 tccagctgac atcaaaccga tcgagaaagt cgaagtcgat ttattgatcg gcccacccat
     5461 atcagctgcc atcatgatcc cgataagtaa tccgaacaat agcccggatg tatttcccaa
     5521 gctattcagc caatttgtca aagcatccaa cacccatgtg atcggttgtt caatcacaaa
     5581 taccatcaaa agcccaataa ttaagatcga taaaaagggg acgacaatca gctgataaat
     5641 agcagaaaat tcttttttta cgctaatttt atttgccaga aaatcacaga tataccctgc
     5701 tagcacgcca ccaatcattc cgccaagaaa acctgaacca cctgtagagg ccagtaatcc
     5761 ggcaacaatt cctggggcaa aacctgcttt tccagcaatc gatgtagcaa tccccgcgcc
     5821 taacgcagga accatcatgg caaacgctgt atctcctccg atccttgaaa atgcagctgc
     5881 taacacatta tagtctgctg agttgggatc agccgctgta atcccaaagg caaaactcag
     5941 tgcgatgatg attccgcctg cgatcacaaa tggaagcatg taattcacac cattcatgaa
     6001 atgattatag attgtcggct ttttctcatt tgcagctggt tctttttcat tagacacaac
     6061 gaatacgcca atatctctca atgcttcgtc tattactact tgcggttctt tgattgctcg
     6121 gcttgtagag acacgcttta cttttttccc ggcaaatcgg tccaattcga tttctttatc
     6181 tactgcaaga atgattgcat ctgcctcttt gatttcttgt tcagataact gattttctgt
     6241 ttttacacct tgtgtttcga attttgcttg atggtttaga tttcttgctg ttacttctaa
     6301 tttttctgcc gacatataag tatgagcaat tccagtcggg caagcagtta caccaacgat
     6361 tttcatgatt attttcctcc tttttcttca ctatcgatac tcggacaaag actctactat
     6421 tttcattctt gactaatctg tctgatgata tctgctggcg tagtggcttc ttttattccc
     6481 tttctaaaat ctgcattcat caatcgtctg gagagtgtgc tgagaatctc tagatgcata
     6541 tctgctgatt tttcaggaac tacgatggca aaaatatatt caattggcat ttcatctatc
     6601 ccgctatagt ccgttaattt ggctgtatga aaaaaacaca gtgctggttt tgtcacatat
     6661 ttgctttttg catggggaat cccaaatcca tcttctattc ctgtggagat ctcctcctct
     6721 cgtttgaata gtgccttaac gactttttga acatttctta ccttatttgt ttttttcaac
     6781 acatccccta ttcgctcaaa taatgatcct tttgaatcta tcgatggatc gaaaacaatc
     6841 agttcttctg cgatcaattg atcaaacctc ataaaaaacc tctcccccac aaaaaatgtt
     6901 aacgttttct tggaactgag gtcagtatag catcgctagc tgtaccgttc aatattatga
     6961 atgaacagag gaaaaagacc catcatccgc gtgtataagt cattaaaatg aacgatctag
     7021 ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct tccttgaaag gaaagcttag
     7081 cttctaattt taggtaaatg gttttttact tcgaaataac ccctcttcat ttcgttaata
     7141 taaggtgttt ttatcaagtg gataattaaa actatatact agtttttcat tgttttgtgc
     7201 attatgatcc tacgactttt tcagcatcaa acgaagtttt aaattggcaa aaaacataaa
     7261 aaaacagact atccctatca gatagtctgc taaacgttga gttatcagcg tttttattat
     7321 tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga aacggtccac acgtccgtct
     7381 gcttgtgtga atttttgacg tccagtgtag aatggatgtg agtcagaagt tacttcgaca
     7441 cggatgactg ggtatgtatt accgtcttcc cattcaactg tttcttgtga agtttttgtt
     7501 gaacctgaca agaatttgaa accagttgtt gaatccatga atactactgg gtggtaatct
     7561 ggatggatgt tttctttcat gattttttcg ctcctttgcc ctgctccatt tgctgaacca
     7621 gagttgattt gtcgatttac aacattaaca tcttaacatg tttcagtcaa ggttgcaatt
     7681 atcttttcag atttctggtc ggattttttt taccaaagga gacatcttga aattctttga
     7741 aaaattgctg attgtttttt gttttacgca aaaagcggat cagttgttcc gtatattcta
     7801 atgaatctcc agtcatattt tttcgaagtt tccaaatttc ttctaattct tcacttggca
     7861 tcaataattc ttcttttcgg gtacctgatt ttttgatatc gatggctggg aagatccgac
     7921 gttcagataa ttcacgtgaa agttgtaatt ctagatttcc agttcctttg aattcttcat
     7981 agatcacgtc atccatacgg cttcctgtat cgactaaagc ggtcgctaaa atcgtcaaac
     8041 tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt cttaggttta tacaatgcgg
     8101 ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg aacgactaag ttataggccc
     8161 gagcaagtct agtaatacta tccatcaaga taacgacatc tcgtttatct tcgactaaac
     8221 gcattgcgcg ttccaatact aattcagaaa ctcgagtatg gttttgcggc tgcagatcaa
     8281 atgtagaaga taccacgtcg cctttcacgc ttcgttctag atccgttact tcttccggtc
     8341 gttcatcaat cagtagaacg attaattcta cttccggata gttttcggtg ataccatttg
     8401 cgatttcttt taatacagaa gttttccctg cttttggcgg tgcgacgatc aatccacgct
     8461 gaccaaaacc gattggagaa aagatatcga tcattcgggt agatagtctt tgcggtgttg
     8521 tttccagttg aagctgtctt tgcggataaa gaggtgttaa ggctggaaaa tgcggacgtt
     8581 cttttgcttc ttccggatct tttccattaa cgctttccac gtgcatcaaa ccataataac
     8641 gttccgattc tttaggtgga cgggcttttc ctgccacttt gtccccattt ctcaaactga
     8701 atcgtctgat ttgtgaggca gaaatataga tatcttctgc acttggtcca taattgattg
     8761 gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa tattccttcc atataataaa
     8821 atccttgttt ctctgcttgt gcacgaatca cagctagaga taattccttt ttattcatct
     8881 tactgtaata aggaatttta aattctttgg cataactata aacttctttt aacgttttgt
     8941 tctctagttc tgccattgtc aaataatcgc tcattcagct cccacttcct ctatcatttc
     9001 aattgatgcc cctaaggctg ttagtttctc gataatatga tcataaccgc gtaagatata
     9061 atctacattg taaatcgtcg ttgtcccttc tgccaatagt ccagcaatca ctaaacaagc
     9121 acctgcccga agatcagaag ccacgacttc tgtgccgtga agtttatttg gaccattcac
     9181 gatgatcata tttccttcca cggtaatatc agcgcccatt cgtgctagtt ccggcacatg
     9241 gttcacacgt ttcgcataga tcgtatcgat gatttctgaa gtgcctgtag tcatcaatag
     9301 taacgcagta agtggctgct gcagatctgt ggcaaatcct ggataaggat aggtcatgat
     9361 gtttgcgggt tttaattctt tcgaggggta aacttcgatt tcatcttcgc tgattttcat
     9421 atggacacca atttcctgca attttgctat aaagctttcc aaatgttcaa agatcacatt
     9481 tttcactttg ataccatttc ctactgcagc tgctaatgct agataggttc ctgcttcgat
     9541 gcggtcagga atcatgaaat gccgacagcc atgtaatgtt tcgactcctt cgatacggat
     9601 cacatctgta cccgctcctc gaacttttgc ccccatatta ttcagtaaag ttgccacatc
     9661 tatgatttcc ggttctcgtg cagcgttttc aattactgtc tgtccttttg cttttacagc
     9721 agctaacatc acattgatcg ttgctccgac tgaaaccacg tccatgaaaa tccgattgcc
     9781 acgcaatcct ttattttctg tccgtaaata catggcacca tgttcatttg tcacttcagc
     9841 acctagagct tcaaatcctt tgacgtgtag gtcaatcggt ctagggccta aataacagcc
     9901 acctggcagg cctactaccg cttcgccaaa tttaccaagc agtgttccca taaaataata
     9961 agaagcacgc aaactattga tttttccgtg cggcatcgga atcgatacga cttctcgagg
    10021 atcgatttct agtatgttgt tggaaaaatg gacagttgcg cccatgatct ctaaaatttc
    10081 aattaatgaa tgaacatctt gtatgtccgg cacaccttcc aagatcactg gggaatcagc
    10141 taagattgca gctggaatca atgcgactgc actatttttt gcaccgctga tcgttacttc
    10201 accagctaaa ggtcggtttc ctttgattac gatttttttc atctgtttcc accttgttta
    10261 ttaatctttt ttcatggata tgcttgtatt ttatcataga taaaaaaaag aaacgagcat
    10321 ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc aaaagtcgtt ctccattcct
    10381 atatatcgta tctgttatgt ggatgcttac gctttgttag ctgaaccgaa ccagtcgata
    10441 tgagatttag catcttcaac gattgctgct ttacctggag ctaataattt acgagggtcg
    10501 aatcctttac cttctttatc tttaccagct tcgatatatt tacgagtagc tgcagcaaat
    10561 gataattgga attcagtgtt aacatttact tttgagatac ccattgagat agctttttcg
    10621 atttgatctt gaggaatacc tgatccgcca tgtaatacta aaggcatgtc tggacctaca
    10681 gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt tccagttttc tggatattga
    10741 ccatggatgt taccgatacc gcaagctaag tagtcgatac ctgtagcaac catttgtttg
    10801 cattcttcaa tatcagctaa ttcgccgtta ccgatgattc cgtcttcttc tccgccgatt
    10861 gaaccaactt cacattcaac tgagatacct tttgcatgtg ctttttcaac aacgtcttta
    10921 gctaatttca agttttcttc aaatggtaga tgtgaaccat cgaacattac tgaagtatag
    10981 cctaattcga tacattccaa agcagcttcg taatcaccat ggtctaagtg gatagcaact
    11041 ggaacagtga tgttcattgc ttcaactaaa tcagtgatta aatctttaca gattttgtaa
    11101 ccacccatgt atttagctgc acccattgat gtttggataa gtactggtgc ttgttttgct
    11161 tcagccgctt caaggattgc ttgagtccat tccaagttgt ttgtgttgaa tccgccgaca
    11221 gcgtaaccgc ctttacgagc agcttttaaa aattcagctc ctgatactac tggcataata
    11281 aaattcctcc taagttttgt aaaactaaat ttgttaagac aattcttaac caacgaccct
    11341 attttaccaa acatttcggt agttttctac taaaatcaat gaaaatgaaa attttcaaga
    11401 agttttttgc aaaaaataaa atatccaata gcgctttaat cgaaaaaagt cttttgcttt
    11461 ctatcttttt tcctacaaat aataacaaat cagaaaaaga gaaaagtgac aagaaaattg
    11521 cttatcccct tctcttgcta cttttctctt tctatcaagt tcaaaaa
//
LOCUS       DASJPH010000090        11337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJPH010000090 DASJPH010000000
VERSION     DASJPH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.090"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(364..1482)
                     /locus_tag="U3995_002261"
     CDS             complement(364..1482)
                     /locus_tag="U3995_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797563.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="U3995_002262"
     CDS             1953..3260
                     /locus_tag="U3995_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1797564.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="U3995_002263"
     CDS             3379..4578
                     /locus_tag="U3995_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1797565.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="U3995_002264"
     CDS             complement(4605..5873)
                     /locus_tag="U3995_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1797566.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="U3995_002265"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="U3995_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="U3995_002266"
     CDS             complement(7090..7593)
                     /locus_tag="U3995_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1797567.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="U3995_002267"
     CDS             complement(7716..8480)
                     /locus_tag="U3995_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1797568.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="U3995_002268"
     CDS             8588..8863
                     /locus_tag="U3995_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1797569.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="U3995_002269"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="U3995_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1797570.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="U3995_002270"
     CDS             complement(10430..10630)
                     /locus_tag="U3995_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797571.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2387 c         2002 g         3547 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaata
//
LOCUS       DASJPH010000091        11232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJPH010000091 DASJPH010000000
VERSION     DASJPH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.091"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..329
                     /locus_tag="U3995_002271"
                     /pseudo
     CDS             1..329
                     /locus_tag="U3995_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(231..947)
                     /locus_tag="U3995_002272"
     CDS             complement(231..947)
                     /locus_tag="U3995_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN1797572.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1071..1256)
                     /locus_tag="U3995_002273"
     CDS             complement(1071..1256)
                     /locus_tag="U3995_002273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797573.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1483..2756
                     /locus_tag="U3995_002274"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="U3995_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..3894)
                     /locus_tag="U3995_002275"
     CDS             complement(2863..3894)
                     /locus_tag="U3995_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797574.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3901..4731)
                     /locus_tag="U3995_002276"
     CDS             complement(3901..4731)
                     /locus_tag="U3995_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797575.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4728..5544)
                     /locus_tag="U3995_002277"
                     /pseudo
     CDS             complement(4728..5544)
                     /locus_tag="U3995_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5541..6365)
                     /locus_tag="U3995_002278"
     CDS             complement(5541..6365)
                     /locus_tag="U3995_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN1797576.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6355..7455)
                     /locus_tag="U3995_002279"
     CDS             complement(6355..7455)
                     /locus_tag="U3995_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1797577.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7633..8418
                     /locus_tag="U3995_002280"
     CDS             7633..8418
                     /locus_tag="U3995_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN1797578.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8451..8834
                     /locus_tag="U3995_002281"
     CDS             8451..8834
                     /locus_tag="U3995_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1797579.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8910..9041
                     /locus_tag="U3995_002282"
     CDS             8910..9041
                     /locus_tag="U3995_002282"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1797580.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9010..9246
                     /locus_tag="U3995_002283"
     CDS             9010..9246
                     /locus_tag="U3995_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1797581.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9324..10295)
                     /locus_tag="U3995_002284"
     CDS             complement(9324..10295)
                     /locus_tag="U3995_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1797582.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            10947..>11232
                     /locus_tag="U3995_002285"
     CDS             10947..>11232
                     /locus_tag="U3995_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1797583.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3338 a         2593 c         2146 g         3155 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa gcaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc
     3241 cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat
     3301 tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg
     3361 gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg
     3421 ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg
     3481 tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa
     3541 tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa
     3601 accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac
     3661 aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat
     3721 ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca
     3781 cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc
     3841 taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg
     3901 tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga
     3961 cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa
     4021 cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag
     4081 catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc
     4141 cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg
     4201 aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac
     4261 taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa
     4321 cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata
     4381 cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag
     4441 cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat
     4501 catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc
     4561 agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg
     4621 taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag
     4681 caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc
     4741 accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact
     4801 gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt
     4861 cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc
     4921 atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa
     4981 gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat
     5041 tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa
     5101 taggtatacg gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     5161 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     5221 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     5281 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     5341 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     5401 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     5461 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     5521 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     5581 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     5641 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     5701 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     5761 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     5821 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     5881 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     5941 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     6001 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     6061 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     6121 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     6181 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     6241 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     6301 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     6361 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     6421 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     6481 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     6541 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     6601 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     6661 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     6721 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     6781 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     6841 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     6901 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     6961 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     7021 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     7081 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     7141 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     7201 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     7261 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     7321 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     7381 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     7441 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     7501 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     7561 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     7621 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     7681 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     7741 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     7801 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     7861 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     7921 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     7981 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     8041 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     8101 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     8161 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     8221 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     8281 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     8341 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     8401 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     8461 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     8521 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     8581 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     8641 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     8701 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     8761 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     8821 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     8881 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     8941 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     9001 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     9061 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     9121 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     9181 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     9241 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     9301 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     9361 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     9421 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     9481 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     9541 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     9601 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     9661 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     9721 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     9781 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     9841 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     9901 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     9961 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
    10021 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
    10081 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
    10141 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
    10201 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
    10261 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
    10321 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
    10381 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
    10441 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
    10501 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
    10561 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
    10621 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
    10681 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
    10741 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
    10801 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg ttatccagac
    10861 tgtagacaaa gtccatttta tggatgatgt ctacagtctt ttcttttata atagatttat
    10921 gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat ggtagaaatc
    10981 aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt cgaaagattg
    11041 atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac tccgatttag
    11101 gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag tatttattcg
    11161 gtatccgttc catgcgacaa accattaagg aagtcgatac caatgtcgct taccgctggt
    11221 ttttaggcta ct
//
LOCUS       DASJPH010000092        11193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJPH010000092 DASJPH010000000
VERSION     DASJPH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.092"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..902)
                     /locus_tag="U3995_002286"
     CDS             complement(9..902)
                     /locus_tag="U3995_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797584.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="U3995_002287"
     CDS             complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="U3995_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1797585.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1677..2778)
                     /gene="prfB"
                     /locus_tag="U3995_002288"
     CDS             complement(join(1677..2705,2707..2778))
                     /gene="prfB"
                     /locus_tag="U3995_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1797586.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2904..5438)
                     /gene="secA"
                     /locus_tag="U3995_002289"
     CDS             complement(2904..5438)
                     /gene="secA"
                     /locus_tag="U3995_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1797587.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="U3995_002290"
     CDS             complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="U3995_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1797588.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6339..7058)
                     /locus_tag="U3995_002291"
     CDS             complement(6339..7058)
                     /locus_tag="U3995_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1797589.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7055..8368)
                     /locus_tag="U3995_002292"
     CDS             complement(7055..8368)
                     /locus_tag="U3995_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1797590.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8449..8769)
                     /locus_tag="U3995_002293"
     CDS             complement(8449..8769)
                     /locus_tag="U3995_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797591.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8877..9164)
                     /locus_tag="U3995_002294"
     CDS             complement(8877..9164)
                     /locus_tag="U3995_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797592.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9291..9935
                     /locus_tag="U3995_002295"
     CDS             9291..9935
                     /locus_tag="U3995_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1797593.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9994..10701)
                     /locus_tag="U3995_002296"
     CDS             complement(9994..10701)
                     /locus_tag="U3995_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797594.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10921..11091)
                     /locus_tag="U3995_002297"
     CDS             complement(10921..11091)
                     /locus_tag="U3995_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797595.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3051 a         2430 c         1956 g         3756 t
ORIGIN      
        1 ttgtggtatt agaatttcaa aaaacgtctc atagaaatga tcgagccaag tgaaccaatg
       61 atcacaccaa tgccgaccat taaaagattg attttccaaa cgatattttg tggacgaatc
      121 aatgaataat gggaacgtaa taatgatgga ttgatcaatt ggaaaaacca tgcataacca
      181 aatgtgatga tcaaaatcgg aagaatcgca ccgatcagac cgatccagcc accttcaagg
      241 aaaaacggcc agcgaatata tccatttttt gcaccgacta aacgcatgat ttgaatttca
      301 cgctgtctag acaaaatcgt gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac
      361 agcagcaatg ccgctgcaac taatccccat gtccgaactg caccagcgat ttggaaaatc
      421 cgatctgagg aagctccacc gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct
      481 tctgctactt gttttgtata agaaggatca gtagcactta ctacatacac atcatataac
      541 gggttattgt caccttcaaa caatttccag gaatcaccca tttcgttttg gatttttttc
      601 aattcctgtt ctttgcttga aaattcaact tttttaacat gatctaagtc attcaattct
      661 ttttctagct ttttcatctc ttcgtctttt gtaccgatat ctacgaatac agatacatca
      721 acgtttcctt ctatatcctg agctaattta gttgcattca tgataactgc catgaataca
      781 ccgactaaag tcaacgtgat cgttaccgca ctgactgaag caactgtcat ccagccgtta
      841 cgtttcaaac tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc
      901 atattcccct tccgcttgat ctcgaataat gcggccgttt tcgatagcaa ttactcggtg
      961 ccgaatcgta tttacgatcg tactgttatg tgtagccatc acgacagtag ttccttgggc
     1021 attgatccga tctaataatt tcatgatttc ccatgagttt tctggatcca gatttcctgt
     1081 cggttcatcc gcgataagta ctttaggcgt attcacgatt gcccgagcaa tagagacacg
     1141 ttgctgttcc ccaccggata attcgctagg aaagactcga actttgtgtt tcaaaccaac
     1201 aaggtccagc acttccatta ctcgtttttt gatatctcga ggttttctgc cgataacttg
     1261 catagcgtac gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa
     1321 caccacgccg atttctctgc gtagataagg aacttcgcgg tttttgattg ctattagatc
     1381 atgacctgcg acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat
     1441 gaatgtagat ttaccggctc cagacggacc aaccacatag acaaactctc cttgttcgat
     1501 gacaatcgag agattgcgga tagcggtcgt accatttgag tacttcttca ttacatcctt
     1561 catttcaatc atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca
     1621 atgtttcaac atgcttgcaa aatcttacaa ttttatttca atttaacaat gagttcttat
     1681 tgtagatgca tttttaaata gccgtcaata aataaatcaa ggtcaccgtc cataactgct
     1741 tggacatttc ctgtctctac gtttgtccga tggtctttta ccatagaata gggatggaaa
     1801 acataagaac ggatctgtga accccagccg atttcttttt gttctcctcg aagtgcagct
     1861 gcttcttgtt ctttcttttc cacttctaat tgataaagct tagcttttaa catccccatc
     1921 gcttgctcac ggtttttcaa ttgtgagcgt tgtgcctgac tagctactac tgttcctgta
     1981 ggcaagtgag tgatccgtac agcagattct gttttattga tgtgctgacc acctgcacca
     2041 cttgctcggt acgtatcgat tttcaaatca tctggattga tttctatatc gatcgtttca
     2101 tcaagctctg gcatcacatc taccgaacaa aaagaagtat gacgtcgttt cgctgagtca
     2161 aaaggcgaaa tccgtacaag gcgatgaaca cctttttctg attttagata accaaaagcg
     2221 ttatgtcctt taatcagcag ggtcacactc ttgatccctg cttcatcacc tgcttgataa
     2281 tctagtgttt ctacttgata gccatgctgc tctgcccaac gtgtatacat acgtaaaagc
     2341 atgctgcccc agtcttggga ctccgttccg ccagcccctg gatgcaattc gacgatcgca
     2401 ttgtttcgat cataaggtcc gtctaacagc atagataatt catacgtctt cattttttca
     2461 tctaatgcat tgatacgtgt ttctgcttct tcttgcattg ctgcatcagg ttcttcttgc
     2521 agcatctcca ccatcaattc gatttcttct agttcttcgg caagagaatg gaattggtca
     2581 taggtttctt tattagcgtt attttcgctg atcacttttt gtgcggcttc gctgtcatcc
     2641 caaaaaccag gttcagccat ttttgcttct gcttccgcga tgtcttcttc cagctgatct
     2701 aagtcaaaga gacctcctga aacttgtgac cttttcctgc atttctgtca ggagatttcg
     2761 aatttcggat atttccatgt tttttccttc tttcactata cttttttcat tatatctagt
     2821 ctaacagaat cagcaaataa cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt
     2881 ctcaccaatc ctgctgtcag aaattagttt gcattttttc catgacagtt cttatatttt
     2941 ttcccgcttc cacaaggaca aggatcattt cttccgacct tgttcacatg gattggctgt
     3001 tttttcgcgc tagtattact attttctttg gcatctccgt cttctgagtg agaagctgtt
     3061 ccttgagaaa cctgttcacg ctgtacgttt tgacggatct cagctttcat aaagattcga
     3121 gtgacttcat actcgatggc gcctaccata tcttcaaaca tcttatagcc ttctgtttga
     3181 tattcgacta atggattgtt ctgtccataa gcacgtaagc cgattgactg acgtaattga
     3241 tccattgcat caatatggtc tgtccattta gagtccacaa cacgcaagat cacgactttt
     3301 tcaaattcca acagttgctc tggaccattt agttgagccg ctttggtatt aaatacttct
     3361 tgtgctcgtt cgtttagata ctctttgatc tcctgagatg tttttccttc gatatctgca
     3421 agagtaatac tatcttcatg gacaagagta ctgccggcaa aatcgatgat actttccaaa
     3481 ttccaatttt ctttctctaa ttgtgtatgc gcatctacta cacgactgat tgtccgtttg
     3541 accatgttca ttagtggctt ggataagctt ttttcttcca tgattacttc ttgtcgctgt
     3601 ttatagatta cttcacgttg ttcacgcatc acatcatcgt attgtaagac gtttttacgt
     3661 gtgtcatagt tatttccttc tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc
     3721 ttgctttgga taacagcatc ttcctcaccg atcttcatcc gatctaagaa agctttgatt
     3781 cgttctgaac cgaatcgttt catcaaatca tcttccaatg acaagtagaa ttgtgacata
     3841 ccagggtctc cttgacgtcc tgcacgtcct cgaagctggt tatcgatacg acgagattca
     3901 tgtcgttctg tcccgataac tgctagacca ccaagttctc ttaccccaag tcctaacttg
     3961 atatctgttc cccgaccagc catgttggta gcaatcgtca cagcaccttt ttgaccagca
     4021 ttcatgatga tctctgcttc tttaaagtga tttttcgcat tcagcacttc atgaggaata
     4081 cgttcttgat tcagcatatc tgataataac tcagatgttt caaccgcaac agttccgact
     4141 aacacaggtt gacctttgcg gtagcgatct ttgatatctt gcacgacagc gtggaacttg
     4201 ctttgtaatg ttgggtaaag taaatctgga cgatcatcac ggataaccgg acgatttgtc
     4261 ggaatctggt atacttggat gttataaatt tctctgaatt cttcttcttc tgttttagct
     4321 gtaccagtca ttcccgcaag tttcttgtac atacggaaat agttctggaa agtgatcgtc
     4381 gccatggttt tcgtttcgtc ttcgatctct acgccttctt tagcttcgat tgcttggtga
     4441 agtccatctg aataacggcg tccatccatg atccgaccag tgaactggtc aacaatcagt
     4501 acttttccgt cttggacaac ataatcgata tctaaaagca tgatatagtt ggctctcaat
     4561 gcttgatcca agtgatgggt caatgcagta ttttcaatgt cgtataagtt atccaaaccg
     4621 aatgtttctt ctgctttttc gatccctgct tcggttaaac tgattgtttt tgattgtaca
     4681 tcgattttat aatcttcttc ttctttcaaa cgtttcacga agttgtctgc tcttgtatat
     4741 aaagctgtgg atttttctgc ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc
     4801 aagattgagt caacttcatc gacaatggca taattcagcg gacgttgtac catttgatgg
     4861 cggtaaacga ccatattgtc tctcaagtag tcaaatccta attcattatt cgtactataa
     4921 gtgatatcac aattataagc ctcgcgtttt tcatccgaag attttgagtt gatattcaac
     4981 cccactgtta atcctaagaa attataaagt tcgcccattt ccgtcgagtc acgggttgct
     5041 aaatattcat tgacggtaac tacgtgtacc ccttcgccag tcagagcatt tagatatact
     5101 ggcatcgttg cggtcaaggt tttaccttca ccagtacgca tttcagggat atttccatcg
     5161 tgaagcacga ttccacccat caattgtacg tggtatggat ataaccccaa tacacgtttt
     5221 gctgcttctc ggacgactgc aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt
     5281 tggtatctgc ccttgaattc agcagttttt gcctgtaatt gttcatcagt taatgctgcc
     5341 atttggtcag cgtatgcttc aacttttttt gcaatgctat ctaaacgttt tagttctttc
     5401 ttatcgtttt cgataagtga acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc
     5461 ttctgtgtat tatccagact atgtagactt tacttttcat tttatcatta gttatagata
     5521 attgaaatat cttaagcgta ttaaagtaag aacttcataa tttattcata tttcatctaa
     5581 aagaggagga aactcaaaaa aacttgcggc agctgtcagt agctgctgca agttttcgtt
     5641 gttatttaag taaggctgat cagtctgttt caatcagtcc gtattttcca tctttacggc
     5701 ggtaaacgat acttgtacca tttgtttctg catcttcaaa aataaagaag ttatgtccca
     5761 gcatgttcat ttgtaagact gcttcttcac tatccattgg tttcaatgaa aggcgtttcg
     5821 tacgtacgat atctaattca gaatcatttt cgttttcgtc ctcttcattg aataaagcag
     5881 cagcatctgc agtattcatg cccgtttcac gggatttacg attaattttt gttttgaatt
     5941 tgcggatttg gcgttccaat ttatcaacaa ccaaatcgat acttgcgtat aaatctggag
     6001 aagtttcttc ggcacgtaat acaagataag gtaatgggat cgttacttcc acttttgctg
     6061 ttttttctgt ataaactttt aagttcacat gcgccgttgc atcgggtgcg tcactgaaat
     6121 agcgttctag tttgcctact tttttctcaa cgtagtcgcg aatcgcttca gttacttcga
     6181 tattttcgcc gcgtacatta tatctaaaca taaaaattac ccctttcgtc taccacaatc
     6241 aagaaggaga aggaccactc tttccctctt cttaattcta ttatatcgaa ctctaggtta
     6301 tttgcaaatt aaatcgctat caaatttcca catctaattc atctcgccaa agaaatggtt
     6361 cggatgattt tcggctgtgc gacctgcaaa caatctgctg cgtggaaaag agtctgtcct
     6421 gttgtataga catcatcaat aatcaatatt tttttatttt gtatcttaga aaaggtgact
     6481 gcaagttcaa aaggttgagg catctccaat ctttcctttc gcgtgttttt tgcttgagcc
     6541 agcagatgct tctctctttt gagtagcatc tgaaaaggaa ttcttgctgc tttcaacatc
     6601 tcgcttactt gattgaatcc tctttctgtt cgcctttttt cagataaagg tattggacaa
     6661 tagatgtatg tcgggtattc tttttttatc tgcgtccatt tatatgcaaa ggtgcctgct
     6721 agtcggatat ctcccataaa cttgtattga ttgagccagg ccttcatatt ttcatcgtac
     6781 acaaaaaagg cacggtgctg aaaatcatag gtcggatatt tcttcttcca ttcttgacag
     6841 tcactgcagt aaacgggaat agactttact ttctttttcg tactctctag attgaaatgg
     6901 attggttttt gacagccaga acagcctctt gtttccaata aatgaaattg tgcgcgacaa
     6961 actgaacaag tctgaggtaa tctgtatttc gaaacaccaa taatctcgct gaatgttaaa
     7021 ttttggaaga tatctttact gcagcaactg cacctcacag catcttcttc ccttcttcat
     7081 tcatttcttt gatttcctga caagcttttc gaatagctgc agttttttct tcatagaaaa
     7141 agtatacttg actgttcgta taaggccctt tacgatcggc tcttcctgcg atttgtacca
     7201 aagaagattt agtaaaaacc ggatgatccg ctcctaaaac gataacggat attctttcaa
     7261 atgtgactcc acgttcaagg atcatcgtgg ttaacagtat atcatatttt gcttctcgca
     7321 ttttctggac tttttccgcc cgctgattgt ctcttgaaga aacatctgtg atctcatatc
     7381 tcggaagagc ctcttgaagt gtggctttca aacgatgcat catcgtgata cttggacaaa
     7441 ataaaaggat atggttttct ttaattaatt gatgaatgat ccgcaccaaa gattttatct
     7501 tcttcttgtt cttgcatttt tgtcgccaat tattcatgaa aaccaagacg ggttcaggaa
     7561 gcaaacgccg atgaaagcgt aaagcaagtt tgtggatgtc aaacgtctgc ttgatttcct
     7621 ttagtaactt tttatcaggt gtggcactta agtagatgaa tcgtccgtct ggtttaatcg
     7681 ctgtttgcac agcatgagct aatcctgcat ccccgttata aggaaacgca tcgatctcat
     7741 cgatcactat taaatcaaag gaccgataaa aacggtatag ctgatgaatc gtacagataa
     7801 caaaagaagt aaaggtataa gccgttgctg tattcccatg gagcaacgtg attttttctt
     7861 ctggaaaaac ataacgatag cgtaaaaaca attcattaca aacatcgact cgcggtgtac
     7921 aaatcgctac tcttcctcca gcttttaaag tttccactaa aataggataa agcatctctg
     7981 ttttccctgc acctgttaca gcccaaataa gatgatgtct tttttctgaa tggcaaagta
     8041 ttctctttga gatttccgtt tgtttttcag taagctgtcc tgtccaaaaa aagacgactt
     8101 ctcgaggttt tgcttcaggc tgatcaaaca aatataaatc ttgttgagtg tcgcatcgcc
     8161 ctaaatatag acaatgtgta caaaaataac cgtaatctgc aagctgtcca tcaatgacgg
     8221 acgtcctcat tccacagcga tgacaaagaa tccagttgcc caaacgttcc actgctgcga
     8281 tcttctcata atttggtaaa tttacctcct cacagtctat tgcttcaata tctctcgtca
     8341 gaagccttct accccaaaaa tgattcatat tcttcgccct aaaaaaatat acgaaaaaac
     8401 aagcgatcta ttttttggat cgcttgttcg atagttatac tgttctattt attcacctat
     8461 gagcgtgatt gcttgatcta acactttttc gccattcatc ataccataat cagccatatt
     8521 gatgacatct aaaggaatgc cttttggttc cagacgtttc tcaaaatcag ctttcataaa
     8581 gcgaacttgt gggcctaata acaacacatc cacgtcttta tttgcaaggt tgttatctgc
     8641 gtcagaagcg gacacagcga aaatatccga gtcgattcct tttgcttcag cagctttttg
     8701 catttttgtt actagtaaac ttgtgctcat accagccgaa catacaagca tgattgtttt
     8761 tttagccata cttttctctc ctttttaaaa caaactatgc aaacgttttc ttcgcgtctg
     8821 ttactctatt atataaaaag aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa
     8881 tcaaatagcc aactagattt cactaattta ttgaacacga catattcttt gtccaaattg
     8941 atgacaacta cttcatagcc tgcatttatc cagccatagg cacctttttc agggtgttta
     9001 taatcatttg cccaccaatc tgtactttcc cttgctgttt tgggaagtcc gttactggga
     9061 aacagcagtt cgtcaaattg ggtaaaggtc aacgttactt gagagccgcc attgttcttc
     9121 aaataatgcg taattgcatc gtactttttc attcgttcct gcatccccag atccacctgc
     9181 ctttgtcttt attatcgcat actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc
     9241 tagaacttta tttatactga ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc
     9301 gctatcgtac aataaaagaa aatggacaaa gtgagattga gatcaaaaaa tcacgcttta
     9361 tctgttctct tagacgaata gaatcagagg accaagcaaa aagtttcatt caagaaataa
     9421 aaaaagaaca ttggaaagcg aaccataact gcagcgcctt catcattgga gaaaaaaatg
     9481 aaatccagcg aagcagcgat gatggagaac caagtggaac ggcgggcgta ccgatgttgg
     9541 aagtcctcaa aaaaaatcaa ctgatcaatg tagctgcagt agtcactcga tattttggag
     9601 gaacgaaact cggcacaggc ggattaatac gagcatacac tcatgcggtc tctcaagcat
     9661 tgtctgacat tggaatcgta gaaggtacct tgcaacagga gatctctgtg atgatcacgt
     9721 atccgcaact aggaaaatta cagaattatc ttgaactcca acattttttt ataaaagaaa
     9781 tccaatatac tgatcaagtt agcgtcatct gtgctgtaga tgaaaaaagt gctgaccact
     9841 ttaaagaatc catcattgag ctgctgaacg gacaagtcca atttgaagag ggttctgttt
     9901 cttatcacga gcaactgata actgatcata tataaaaaca ggtgattctg attctctttt
     9961 atttctaata aggaatcagg gatcacctgt tttttatttt tccaaataaa actcaaaacg
    10021 atttcctacg tattgcgtac gcacatactc aaacggcgta ccgtcttcaa aataagaaat
    10081 ctggcgcaaa cgaagaactg catctcctcg ttttacctct aaatagtcag caattttctc
    10141 agaagccaac acagcagtaa tcgtttgatt cgctggaccg atctttttcc cgcctttttc
    10201 ttctagcgtg cgatacaacg attcagtaat ctctgctttg ctgtagccat cgatcagctt
    10261 ttgtggaaca ctggcaactt caaaacaaat agggacatca tctgcgaaac gaatacgttc
    10321 cattcgcaag atcgtgtcat ctttactcaa gtttagtttt tccatttcac ttgagcttgg
    10381 cgaagtcaag aaataagaga ttgtccgact agacggtact cgattttgtg attccataat
    10441 ttctgtaaaa cttgttgcac cagacatttt ttcttgtact ttttctcgcg caacatacgt
    10501 acccgaaccg atctttcttt ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct
    10561 caacgtcata cggctgacat tgaatttcac tgctaattct ctttctgaag gaagacggtc
    10621 tccgactttc catataccct gttcgatttc attttttaat tggtcatgaa tctgtatata
    10681 tacaggtaat tgattcgaca taacgtacac ccgtcctttc gtttcttcta ttataactgg
    10741 tatatacctg attcacaact gaaaaagata aaggtttcta tccatcaaca gaggatttag
    10801 acacacatcg ttctccctac tcctattgtc agaaacaatt ttttagatat gttcaaccta
    10861 gaaaataaca aaagacccta aaatcgatga aaatgccgac ttatggatct tttgttatta
    10921 ttagcggttt ttagccatcc atttttttgt atgattcgtt gggcgttttg attttacctg
    10981 gaccgctttt ccgccttttt tcggaatatt cacttcaata ttgtattttt ctgatttgac
    11041 caaatcatat aacttttcag ctgcttcgtc tactttttct ttccatttca tgtggatccc
    11101 tccaattctt gtagttaact taactctatc ttaattcagc agaatcttca aggaacaagg
    11161 tagcaaagca aaaaaaagag ctctcgaact aac
//
LOCUS       DASJPH010000093        11121 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJPH010000093 DASJPH010000000
VERSION     DASJPH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.093"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(83..823)
                     /gene="truA"
                     /locus_tag="U3995_002298"
     CDS             complement(83..823)
                     /gene="truA"
                     /locus_tag="U3995_002298"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1797596.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1014..1649)
                     /locus_tag="U3995_002299"
     CDS             complement(1014..1649)
                     /locus_tag="U3995_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1797597.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="U3995_002300"
     CDS             complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="U3995_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1797598.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3235..4233
                     /locus_tag="U3995_002301"
     CDS             3235..4233
                     /locus_tag="U3995_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797599.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4246..6264
                     /locus_tag="U3995_002302"
     CDS             4246..6264
                     /locus_tag="U3995_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN1797600.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6324..7040)
                     /locus_tag="U3995_002303"
     CDS             complement(6324..7040)
                     /locus_tag="U3995_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1797601.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7267..7809)
                     /locus_tag="U3995_002304"
     CDS             complement(7267..7809)
                     /locus_tag="U3995_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1797602.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7814..8041)
                     /locus_tag="U3995_002305"
     CDS             complement(7814..8041)
                     /locus_tag="U3995_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1797603.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8120..10000)
                     /locus_tag="U3995_002306"
     CDS             complement(8120..10000)
                     /locus_tag="U3995_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1797604.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10189..10803)
                     /locus_tag="U3995_002307"
     CDS             complement(10189..10803)
                     /locus_tag="U3995_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797605.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10945..>11121
                     /locus_tag="U3995_002308"
     CDS             10945..>11121
                     /locus_tag="U3995_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1797606.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         3452 a         2237 c         1801 g         3631 t
ORIGIN      
        1 atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt
       61 ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg
      121 cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt
      181 ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt
      241 tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag
      301 ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa
      361 tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact
      421 cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa
      481 cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt
      541 cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt
      601 ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc
      661 cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata
      721 ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat
      781 atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct
      841 gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg
      901 cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa
      961 aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt
     1021 ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt
     1081 tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt
     1141 ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg
     1201 tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca
     1261 ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca
     1321 tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg
     1381 gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca
     1441 ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt
     1501 acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga
     1561 taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag
     1621 aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt
     1681 ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca
     1741 tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac
     1801 gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg
     1861 cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca
     1921 ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat
     1981 tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac
     2041 ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag
     2101 gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg
     2161 caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca
     2221 atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa
     2281 agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt
     2341 taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga
     2401 tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg
     2461 attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt
     2521 gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa
     2581 aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag
     2641 attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt
     2701 gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta
     2761 caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct
     2821 tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa
     2881 ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa
     2941 atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac
     3001 gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta
     3061 acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac
     3121 aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt tgttttttga
     3181 gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt
     3241 ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc
     3301 ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca
     3361 gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa
     3421 acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa
     3481 atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc
     3541 acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat
     3601 tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg
     3661 cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt
     3721 aaatatctac tattattttt aacaattgga ttagttggct gctctactct atttaataaa
     3781 gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt atttttaatg
     3841 ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta
     3901 tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc
     3961 atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg
     4021 tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa
     4081 ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct
     4141 ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca
     4201 tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt
     4261 atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct
     4321 tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt
     4381 cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc
     4441 gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact
     4501 cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta
     4561 ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg
     4621 ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat
     4681 ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc
     4741 gtgggcggat tgatccttct cctattattg attactagta ttattgtaca acgtagacta
     4801 caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta
     4861 ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg
     4921 tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac
     4981 ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc
     5041 tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc
     5101 aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc
     5161 ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc
     5221 aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga
     5281 acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg
     5341 ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat
     5401 ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa
     5461 gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga taaattaact
     5521 tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc
     5581 ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa
     5641 aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga aggaactgct
     5701 gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat
     5761 acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt
     5821 ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta
     5881 gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct
     5941 acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg
     6001 aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca
     6061 ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt
     6121 acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta
     6181 gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg
     6241 gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat
     6301 gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca
     6361 tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga
     6421 ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc
     6481 tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct
     6541 tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc
     6601 cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga
     6661 ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct
     6721 aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa
     6781 gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt
     6841 ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg
     6901 ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat
     6961 tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt
     7021 actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta
     7081 tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt
     7141 ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta
     7201 ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat
     7261 agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag
     7321 tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg
     7381 caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg
     7441 gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg
     7501 accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc
     7561 attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata
     7621 ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg
     7681 gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc
     7741 agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac
     7801 tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt
     7861 ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt
     7921 tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt
     7981 cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca
     8041 tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa
     8101 aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata
     8161 aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag
     8221 ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa
     8281 tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca
     8341 acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc
     8401 ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat
     8461 ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt
     8521 cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct acataatcag
     8581 cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact
     8641 tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga
     8701 tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat
     8761 cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg
     8821 cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat
     8881 ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc
     8941 gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt
     9001 catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag
     9061 ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga
     9121 ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa
     9181 tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac
     9241 taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc
     9301 gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa
     9361 ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg
     9421 ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac
     9481 cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa
     9541 tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt
     9601 cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag
     9661 ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa
     9721 acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag
     9781 caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga
     9841 tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga
     9901 caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga
     9961 tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt
    10021 cattcttacg agactaagta tagatactta tccatcataa aaaattgacc actatcaagt
    10081 ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag
    10141 actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat
    10201 tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat
    10261 tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc
    10321 tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt
    10381 accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg
    10441 cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt
    10501 actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc
    10561 aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc
    10621 gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata
    10681 tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc
    10741 aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt
    10801 catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt
    10861 gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga
    10921 tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa
    10981 aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact
    11041 atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac acattataag
    11101 gatatctatg atctatatga t
//
LOCUS       DASJPH010000094        11114 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJPH010000094 DASJPH010000000
VERSION     DASJPH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.094"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U3995_002309"
     CDS             complement(<1..832)
                     /locus_tag="U3995_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1797607.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     T"
     gene            complement(1400..2467)
                     /locus_tag="U3995_002310"
     CDS             complement(1400..2467)
                     /locus_tag="U3995_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1797608.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2487..2966)
                     /locus_tag="U3995_002311"
     CDS             complement(2487..2966)
                     /locus_tag="U3995_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEN1797609.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2983..3444)
                     /locus_tag="U3995_002312"
     CDS             complement(2983..3444)
                     /locus_tag="U3995_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1797610.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3458..4708)
                     /locus_tag="U3995_002313"
     CDS             complement(3458..4708)
                     /locus_tag="U3995_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN1797611.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(5086..6537)
                     /locus_tag="U3995_002314"
     CDS             complement(5086..6537)
                     /locus_tag="U3995_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1797612.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6858..7622)
                     /locus_tag="U3995_002315"
     CDS             complement(6858..7622)
                     /locus_tag="U3995_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1797613.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7650..8348)
                     /locus_tag="U3995_002316"
     CDS             complement(7650..8348)
                     /locus_tag="U3995_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1797614.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8360..9139)
                     /locus_tag="U3995_002317"
     CDS             complement(8360..9139)
                     /locus_tag="U3995_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1797615.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(9155..10099)
                     /locus_tag="U3995_002318"
     CDS             complement(9155..10099)
                     /locus_tag="U3995_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1797616.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(10145..10924)
                     /locus_tag="U3995_002319"
     CDS             complement(10145..10924)
                     /locus_tag="U3995_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797617.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         3266 a         2286 c         1625 g         3937 t
ORIGIN      
        1 aagtacgaca gcgtcccaac aaaagtctcc ttgttttgtt tacaaatatc tcattttcta
       61 atatatagct taaatcgcct tcaatttttt taactaattc aatattacaa tcgaactcat
      121 tatctctaaa attatgtgtc aatgaattat tattattaca tctataataa tagatacaat
      181 ctttaataaa acttgttatt tttgaatgtt gcaaatatga aatatggaat atgatatctt
      241 cagatatata atccctttct gacggaaact tgatattgtg ctgttgaatt atctcagatg
      301 aatacagcgc atgccaaact gacattggca atataaaatc gactttataa ctcggctcag
      361 tgccaatcat tccaagcaac actttttcaa ttatagcgtt atttgtaaat atcgtactat
      421 catatcttgc tggctttgaa ataatattat tatcatcata atattcataa tatccagcat
      481 aacatgtgtc tgcattactc ctttttatac catttaacat tgcttcaata taattagctg
      541 caacaaaatc atcagaatca ataaaagcaa catattttcc ttttacatgt tcaagtcctg
      601 tatttcttgc caatcccaag ccagcattat ttttatgaat aatacgaatc cttgaatcct
      661 ttaatgcata gtcttcacat attttattag atgaatcctt agatccatca tccacaatta
      721 taatatctag gttcttatat gtttgattac acactgattc caaacaactt cctacatact
      781 tttcaacatt atatacaggt atgattacac taattagatc actcgaattc atccttctat
      841 cctttcactc acgttttctc aattagtcaa tgacggcatc aacgattttc ttaaattctc
      901 tgaaaaaaat agactctgat cttgatgatg tgaatgacat actatcttct tctagaaaaa
      961 ttatttcctt taaattattt attaaatcaa taatatgatc ctcgtaagta tgctttccct
     1021 tggaataatg aaaatatact tttgtattac acaatattgc aattttattt cttaatatga
     1081 ttatttcact cattcattac tgccacatat acacaactta taaccacccc ctgcaaagtt
     1141 tagggtaagt tatcagataa tatcttcctc ttcatcatcc agaatacaaa atctatttat
     1201 ttatagaaat atcattaatg tttttatata atgtaccgtg cttgtactct tgctctatta
     1261 caagcactaa aacactacta ttcatttgaa ttcttacacg gataaaaatt tacatcgtct
     1321 atttcaatat tttttctctc tattttattc acttaataag ttcattacat aatcgaaaca
     1381 ttttttattt tccttgtcat cactccttgc tccctttttg atacagattt aaaattctat
     1441 caaagaaatt tccatcgtta aatttcgtta ttgccaaatc cttcgaacaa cgtgaaaatt
     1501 ttacgtattt atctttatca ttgattttta gtattgcaga tgtcaacgct gatacgtcac
     1561 caggttgaaa caaatatcca ttttctccat cacgtactgc tgaagaaata tctcctacat
     1621 ctgtagcaat tacaggcatt ccataagaaa tcgcctctag aatagatata ggcaatccct
     1681 cgttatagga cggtaaaatc atcacatttg atcttctata gcactcctcc tttttttctc
     1741 ccacaaccca acctgtaaat tcaaaatacg attgcaaatt atattcttct atcaattctt
     1801 tgagcgatat ttcttcatca cctgctccag caatctcaaa aataaacttt ttttctggat
     1861 attgttctgc aatgttttta actgcttcta ccaaatcgtg tacacctttt cgctttatca
     1921 aaactcccat aaacagaaat cggcacattt ttgaattcca ctttatggta tattctggaa
     1981 ctacaataga attttctaca acaacagttt tacaatttgg ctcaatttct ttaattatct
     2041 tcttccattt ttctccaagt acaatcgtgg aatcagactc tcttaacagc aagcgaatct
     2101 gattttgttt ttttacagaa acactatcat accatttttt aaattccgat ccatgaagat
     2161 ggatgattac aggaatattt aataatctaa accacttatt cagcacatat ttcctactaa
     2221 aactccctct gtacgacatg tgcatatgaa taatattagg tttactcaat aaagagatac
     2281 ctataatttt agcaaatgca tacataaaat ataaagattt aaatattgca tttccaccct
     2341 tataactcgg tataaaagtc atctcgatat tctttttttc ccaattaaaa ttgagcattt
     2401 gtgtaattat acttgtaatt ccacctttta ccgagctgtc attaccaatc ataagtactc
     2461 tttgcataac gctttactcc ttatatttat attttttcta ttaatacctc tatcgaatta
     2521 ataatcgttt ccgtgttaga tacatacttg ttaaacatcc gaactcttgt ttctttaacg
     2581 gcatcttcta aattttctaa ttcataacaa ggatagataa tattcatctc atcaaattgt
     2641 ctaattaact gcagttggtg atcatcgaca tgctcaccat acttagccaa tcgaggaaca
     2701 gcaatgaccc ttttaccttt ttttacggcc ccaattatag cacctgtacc tccatgagta
     2761 atcaccattt ctgctttttg catagtttca gtaaactcat ccctatttag aaatgcttta
     2821 taggaataat ttagtggttt ataatcacta taaccactct gtgcaaatgt actttcgatt
     2881 attactcctt tttcaattaa atcatcaata gcttttaaca atctatcaaa ttgaaatttt
     2941 tgtgaaccta atgttataaa tatcattttt ttctcctatt atttaatata tcccacccaa
     3001 ataaatagca ttaggataaa attgtagcat agtttcccac tgaacataaa attgatctgc
     3061 aaaatgatac atcaattttc ctgtctgagt agggctggtt acttttgcaa acgattcaat
     3121 ataaatcagt ttttttccca tcatttttgt tattaagcag atagggatca tagctaaaac
     3181 acctgtacaa atcacgagat ccggtttttc ctttatatat ataataattg atttgaataa
     3241 attaataagt atagtacaca aaaaaaactt ttcttttcta tttacttgtc tcatgtagta
     3301 cgttttttcg ccttttgact cagctgaata ttctgtctgt tctgttataa caaaactctc
     3361 atacttctcc atcagtggtt ttaacatcat taactgttca taatgccccc cagaggaagc
     3421 cgcaaaacat atctttttgt tcatattaac ctctctatca gtcacgctga aaaatatctc
     3481 tagtgtaaac tttttcttta atatcatcca agcaggtatc atatcgattg gcaataatag
     3541 aatcacacat cgccttaaac tgctctaaat cgttgataac cttacttccg aaaaaagtct
     3601 ctccatcttt cagtgcaggc tcataaatca ccacagtcgc acctttagct tttacgcgct
     3661 tcataactcc ctggattgaa ctctgacgaa aattatcact gttgctcttc atggtcagcc
     3721 ggtatatacc gattaaaact ggattttccc tgttgacatc atactgatta tattcagcgt
     3781 agttgtaata gcccgccatc tgaagaacac gatctgcgat gaaatcctta cgggtacgat
     3841 tagattcaac gatagcctca atcaaatttc caggaacatc tgcatagttt gccaacaact
     3901 gcttggtatc ttttggcaag caataacctc catatccaaa ggatggattg ttgtagtgag
     3961 ttccaatacg aggatcgaga caaacaccat tgattatctg ctgagtattc agccccttca
     4021 tttcagcata agtgtctaat tcattgaaat agctgactcg cagtgctaaa taggtatttg
     4081 caaacagctt tactgcttcg gcttcagtga atcccataaa aagagtatcc acgttctcct
     4141 taattgcact tttctgcaga agtgttgcaa aaacatgagc agcatttaac aagcgctcat
     4201 cagtcatatc agtaccaaca ataatacgag atggatacag attatcatat aaagccttgc
     4261 tctcgcgcag aaactctgga ctaaaaataa tgttcttact ccccgttgtc tcacggactg
     4321 attcagtgta gccaacagga attgtagact tgatgaccat gatggtatca ggattcacgt
     4381 ccatcacaac ctcaataact gactcaacag cagaagtatc gaaatgctga gtacgactgt
     4441 catagttagt cggtgtagca ataacaacga aatctgcgga tttataagcg ctccttgcat
     4501 ccaaagtagc agttaagtca agttcttttt ctgtaagata cttttcaata tattcatcct
     4561 gaattggaga cttccgttta ttaatcagtt caatcttttc agggataatg tctaccgcgg
     4621 ttacctggtg atgctgcgcc aatagtgtgg cgatactcaa accaacataa ccggtgccgg
     4681 ctactgcgat gttgtacttt ttcttcacaa tatctcctct ttcctctctt aagtatggag
     4741 aatgttactc ctacggctat tcatcatgtc aaaataacac agcttagaat gttgataaaa
     4801 taattttcca gaatgtgata aaatctcttt catttataaa ccacacatct aattttatct
     4861 attctccaca agacatgtag agtattcttt aataacaaga tacgacctta ctaaatactt
     4921 caaaaaatta tcatatttaa ttcataaata taaatcttca ttttaaatgt atgcccaaaa
     4981 atttatctgt aatgcgtcat caaaatctag attccaaatc tttatcaatt tttatccact
     5041 cattctacca ctttagtaat attttctatt tataacactt tcgtcctaca ttgatccccc
     5101 cctctgaagt acagttttta ctgtcttaaa taaaatttta aaatctaacc ccatactcca
     5161 attttttata tattccgtgt caagttttgc tacttcttca aaatcagtaa tatcacttct
     5221 accacttacc tgccacattc cagtaattcc tggcttaata gcaattcttg cacgatgatg
     5281 ataatcatat tcttcatact ctgatttaag gcatggtcga aatcctataa ggctcatctt
     5341 tcctgttaga acgttgaaaa attgagggaa ttcatctaaa gacgtggatc taataaattg
     5401 accgattcct gtcttttttg ttccatctgg aagtatttta tttccgataa ttcttggatc
     5461 aaactccatc ttaaacataa gattattgct catcttattt tctttcatta attctacttt
     5521 tctttcttct gcatccatat acatacttct aaacttatac atcttgaacg gtttaccatt
     5581 ctgacctata cgttcttgtg caaagaaaat tgggcctggt gaattaatat aaataagtgg
     5641 agcaataaag ataaaaataa ttcccgttgc aatacatcca acaatacctg caagaatatc
     5701 aattgttcgt tttataaatg cctgttgagt tgtcataaca ttgatacttg ttgtaagtac
     5761 tgtatattct cctatacgac caatagattg ttttcccaaa gcactatctg atacttttgc
     5821 aagattcaaa tgtacagtaa ggcccatttc agagcatcgt tcaataaatt ctttcataca
     5881 cgaagtactt ttattcaaaa taataaagac ttcatctacc catccctgac ataagtaatc
     5941 cgctgcattt ttggaatcag aaacaactgt cactccctca acatcttcac caatcatgct
     6001 tttatctatg ataatgacac catttatttt atacatttta tagttatgct ctttcacatt
     6061 acgaataact acttcagcaa tactactaga tgtaactata ataagtgatt tgttcccctc
     6121 atttttcatt ttatgtttca agtaatgttt ccataagagg cgagttatat atgtaattac
     6181 aacgtataaa cctcccatca agaaaagaac gactctagaa taatcatttc caccctgaac
     6241 actaatgaga tacaaacttc ctaacagcac caacataatc gcttgcttta cagaagaagc
     6301 aaattctata tagtaccctc tttttaatac acctctatat gcttcaaaca tgaaaataac
     6361 taaaagatct gccagttcaa tgaagactgc catgttttga tacattggaa tcacataagg
     6421 attccaatct acctggcgta agacatacga tagataaaaa gcaagctgga tacacaataa
     6481 atctaaaata ataaaatcaa tatgtttaac ccatcccctt gaacttttct tgtacattcc
     6541 tttttactcc cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc
     6601 tttctgaagg attattgact cgacaaattg gcttgtggat attcttatat atagaacatc
     6661 cgtaagccaa cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa
     6721 gtatccaaga aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa
     6781 aatcatcata aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt
     6841 gatttacgaa taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact
     6901 ggataactga tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact
     6961 ttttccttac caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc
     7021 ttagagaccc catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt
     7081 ttggctgttt tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg
     7141 gccaaacatc ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg
     7201 ttgcgttctg gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc
     7261 cgttctgcat ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct
     7321 aaatctgtaa ataatatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt
     7381 acttcttgtc cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac
     7441 gaagcgacta gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt
     7501 ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt
     7561 gcttctaagt tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc
     7621 atttctcttg tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg
     7681 ctatgttctg ctccgttgta cacaacacct aagactcgtg cttgtaccat ctccagcagt
     7741 tcttttgctt tagtcaagga ttctttacgt gacatatttt cacgtacgac caataaagtg
     7801 ccatccgttt ttaaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg
     7861 aatatcacca cgtcaaataa ctgtcttgct tcggatagga tctgatccat tcttggcgag
     7921 cttaacaatt ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg
     7981 actggcgttc gttggatcac atctgctaca cttcctgatg aactaagtgc tgtacttaat
     8041 ccactggcat tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg
     8101 actagtagaa ctcgttgacc tgatttagca aaaacgactg caatgtttgc tgcagtggtg
     8161 gatttccctt ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct
     8221 gcggaggcaa actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat
     8281 gggtttgtta atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt
     8341 cgtgccattt ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat
     8401 cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt
     8461 ctttcgctga catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat
     8521 atttttcatc tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta
     8581 ccatcattcc taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg
     8641 tcgtattggc aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg
     8701 cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg
     8761 tacttattgc ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat
     8821 ctttgatttg ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt
     8881 cattgagcac taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg
     8941 tattgacatc attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt
     9001 gtgaactgta ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag
     9061 caaacattga gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca
     9121 agctgattgt ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat
     9181 cttttaattc ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat
     9241 tatcttgcat gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc
     9301 gataatctaa agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact
     9361 ctagagcatt caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg
     9421 ctacgccttc tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt
     9481 cataacgcat acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg
     9541 gaaagtcata gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg
     9601 catcaacgag ttcctttagt cctttcatat tgatagagac atagtgatcg attgggatgt
     9661 ctacatattt ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt
     9721 tgatcttgtc ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga
     9781 cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc
     9841 cttgatcgac ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt
     9901 cttgtttgtc cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt
     9961 aagttttctc aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg
    10021 ctaatactaa tacgataaga actgctaaaa taatcaaaaa tatttttttc cctcttgatt
    10081 ttcttcttcg tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta
    10141 actgtcaata gcttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg
    10201 cgtatgcggc tcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat
    10261 attgatcgta agtagcggta gctgttactt gaccgtcttt cagcatcgta tcgaatagct
    10321 gatctagttg cgtgacttgt gcaatttggg tatcagcgaa tcctagatat tgcataacga
    10381 ctgttttcca agcactatcc ttaaaaaagc tcatattttc acgtacatgg gcgacatctg
    10441 cacttgccaa cttttctttg atgatgacat catccacagc ttgctgccag tttgatacgt
    10501 ttttagtaaa taatttattg acagccgtct ggatcttcag ttttgattct gcatcctcta
    10561 actgcgttaa gactgctttc ttttcttctt ggatcgcagc agcttcttta ggtaattctg
    10621 attccttgat gtcataatca tctgccgaaa gtttgataga agatagttta tgtgacaaat
    10681 ttgaaactgt ttcttctgtt atttcaggat ttaggaaaat tttttctttt gtactgtaca
    10741 gcttatctac ttcttttgat aaagaggtca cggtgtcttt ttgattggtg atggcagatt
    10801 ctgcgtaatt gatccgttga tgatattgcc gctgattatt gagatggtat ccaaacaagc
    10861 aaatcacaat agcgcctgtg cctaccgcca cagtagccaa taacttgacg aacggctttt
    10921 ccatagattc actcctaaat attgatgata gataagtaaa gatgtgattc attcacatca
    10981 gaatgagcgc tttttatttt aaaagcataa ttaaactaat atattcatag aaatatttat
    11041 aaacaggttt tccctatata atcataccac atacagtttt tttgactagg gaaattccta
    11101 atttaaaagt aaat
//
LOCUS       DASJPH010000095        11096 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJPH010000095 DASJPH010000000
VERSION     DASJPH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.095"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..139
                     /locus_tag="U3995_002320"
     CDS             <1..139
                     /locus_tag="U3995_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1797618.1"
                     /translation="IDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLKEKTVLP
                     K"
     gene            178..1128
                     /gene="manA"
                     /locus_tag="U3995_002321"
     CDS             178..1128
                     /gene="manA"
                     /locus_tag="U3995_002321"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1797619.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            1222..2925
                     /locus_tag="U3995_002322"
     CDS             1222..2925
                     /locus_tag="U3995_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1797620.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            2927..4960
                     /locus_tag="U3995_002323"
     CDS             2927..4960
                     /locus_tag="U3995_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1797621.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            4971..5411
                     /locus_tag="U3995_002324"
     CDS             4971..5411
                     /locus_tag="U3995_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797622.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(5611..6738)
                     /locus_tag="U3995_002325"
     CDS             complement(5611..6738)
                     /locus_tag="U3995_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797623.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(6741..7418)
                     /locus_tag="U3995_002326"
     CDS             complement(6741..7418)
                     /locus_tag="U3995_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797624.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            7682..9448
                     /locus_tag="U3995_002327"
     CDS             7682..9448
                     /locus_tag="U3995_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1797625.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            9463..9900
                     /locus_tag="U3995_002328"
     CDS             9463..9900
                     /locus_tag="U3995_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1797626.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            9913..11064
                     /locus_tag="U3995_002329"
     CDS             9913..11064
                     /locus_tag="U3995_002329"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1797627.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         3753 a         1953 c         2291 g         3099 t
ORIGIN      
        1 gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga
       61 tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa
      121 aacggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg
      181 caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta
      241 cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt
      301 gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat
      361 gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta
      421 ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc
      481 tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct
      541 gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag
      601 atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat
      661 ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa
      721 acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa
      781 ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa
      841 acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta
      901 gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa
      961 caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga
     1021 aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg
     1081 gcggtccaag gtgaactagc aatcattgcg actgaaccag gcaaatagtt cccagatagc
     1141 tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata
     1201 aatagaagta tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg
     1261 tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta
     1321 agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg
     1381 cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc
     1441 agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct
     1501 tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg
     1561 ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccacgtgaa attcaaggct
     1621 ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata
     1681 attggaatcg tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt
     1741 gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta
     1801 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
     1861 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
     1921 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
     1981 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
     2041 ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt
     2101 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
     2161 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
     2221 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
     2281 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
     2341 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
     2401 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
     2461 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
     2521 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
     2581 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
     2641 aaaaagtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
     2701 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
     2761 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
     2821 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
     2881 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
     2941 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
     3001 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
     3061 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
     3121 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
     3181 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
     3241 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
     3301 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
     3361 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
     3421 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
     3481 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
     3541 gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
     3601 attgacgatg gagcgtgtga ctaaaaatca tttactgaca aatgagacat ataatagtct
     3661 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
     3721 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
     3781 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
     3841 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
     3901 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
     3961 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
     4021 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
     4081 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
     4141 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
     4201 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
     4261 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
     4321 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
     4381 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
     4441 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
     4501 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
     4561 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
     4621 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
     4681 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
     4741 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
     4801 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
     4861 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
     4921 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
     4981 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
     5041 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
     5101 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
     5161 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
     5221 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
     5281 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
     5341 aagtaatggc gctcagtgat gcgaaaacca ttgaagaagt ccaaatgatt tttgaacatg
     5401 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
     5461 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
     5521 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
     5581 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
     5641 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
     5701 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
     5761 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
     5821 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
     5881 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
     5941 gaacagaaag ctctggaaaa gatactcata catcggaagg attcgtatct cctgaccaat
     6001 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
     6061 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
     6121 gatgctatgt ttctttacca tactatccgg taatagattg actagagaga ttacctgctt
     6181 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
     6241 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaaggga gatgcgctgg
     6301 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
     6361 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
     6421 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
     6481 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
     6541 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
     6601 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
     6661 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
     6721 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
     6781 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
     6841 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
     6901 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
     6961 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
     7021 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
     7081 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
     7141 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
     7201 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
     7261 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
     7321 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
     7381 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
     7441 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
     7501 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
     7561 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
     7621 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
     7681 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
     7741 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
     7801 aacggctcta tttatcccag atggttattt accaaatgag gctttggcta ccatggtaag
     7861 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
     7921 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
     7981 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
     8041 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
     8101 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
     8161 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
     8221 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
     8281 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
     8341 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
     8401 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
     8461 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
     8521 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
     8581 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
     8641 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
     8701 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
     8761 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
     8821 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
     8881 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
     8941 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
     9001 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
     9061 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
     9121 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
     9181 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
     9241 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
     9301 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
     9361 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
     9421 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
     9481 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
     9541 cagatgcttg tagaagcagg gggcgttgat gaggaatata tcgatgccat ggtagataga
     9601 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
     9661 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
     9721 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
     9781 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
     9841 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
     9901 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
     9961 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    10021 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    10081 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    10141 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
    10201 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
    10261 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
    10321 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    10381 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
    10441 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
    10501 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    10561 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    10621 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
    10681 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
    10741 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    10801 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
    10861 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
    10921 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
    10981 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    11041 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaa
//
LOCUS       DASJPH010000096        10760 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJPH010000096 DASJPH010000000
VERSION     DASJPH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.097"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="U3995_002330"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="U3995_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="U3995_002331"
     CDS             complement(1437..2279)
                     /locus_tag="U3995_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1797628.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="U3995_002332"
     CDS             2604..3020
                     /locus_tag="U3995_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1797629.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="U3995_002333"
     CDS             3017..3487
                     /locus_tag="U3995_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797630.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="U3995_002334"
     CDS             3501..4274
                     /locus_tag="U3995_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797631.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="U3995_002335"
     CDS             4288..5100
                     /locus_tag="U3995_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797632.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="U3995_002336"
     CDS             5154..6119
                     /locus_tag="U3995_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1797633.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="U3995_002337"
     CDS             6255..8978
                     /locus_tag="U3995_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797634.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="U3995_002338"
     CDS             8991..9623
                     /locus_tag="U3995_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1797635.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9828..10055
                     /locus_tag="U3995_002339"
     CDS             9828..10055
                     /locus_tag="U3995_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN1797636.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10124..10390
                     /locus_tag="U3995_002340"
     CDS             10124..10390
                     /locus_tag="U3995_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1797637.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="U3995_002341"
     CDS             10380..10649
                     /locus_tag="U3995_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1797638.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta
//
LOCUS       DASJPH010000097        10711 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJPH010000097 DASJPH010000000
VERSION     DASJPH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.098"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..957)
                     /locus_tag="U3995_002342"
                     /pseudo
     CDS             complement(1..957)
                     /locus_tag="U3995_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1448..2386
                     /locus_tag="U3995_002343"
     CDS             1448..2386
                     /locus_tag="U3995_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1797639.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2516..3163
                     /locus_tag="U3995_002344"
     CDS             2516..3163
                     /locus_tag="U3995_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN1797640.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3856..4794)
                     /locus_tag="U3995_002345"
                     /pseudo
     CDS             complement(3856..4794)
                     /locus_tag="U3995_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4794..5411)
                     /locus_tag="U3995_002346"
     CDS             complement(4794..5411)
                     /locus_tag="U3995_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1797641.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5425..5595)
                     /locus_tag="U3995_002347"
     CDS             complement(5425..5595)
                     /locus_tag="U3995_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797642.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5943..7433)
                     /locus_tag="U3995_002348"
     CDS             complement(5943..7433)
                     /locus_tag="U3995_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1797643.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7476..7775)
                     /locus_tag="U3995_002349"
     CDS             complement(7476..7775)
                     /locus_tag="U3995_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797644.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8141..8425)
                     /locus_tag="U3995_002350"
     CDS             complement(8141..8425)
                     /locus_tag="U3995_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797645.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8944..9429)
                     /gene="vanZ"
                     /locus_tag="U3995_002351"
     CDS             complement(8944..9429)
                     /gene="vanZ"
                     /locus_tag="U3995_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1797646.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9582..10493)
                     /locus_tag="U3995_002352"
     CDS             complement(9582..10493)
                     /locus_tag="U3995_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN1797647.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3308 a         1864 c         1658 g         3881 t
ORIGIN      
        1 acttttttct gcttcttttt ctttctgttc ctggtaatca gaaaaattta gtttttctat
       61 atcgttaggt acagcttcca gtaaatgaac aatgaatttt ttgatattcg taataaagtt
      121 ctcttgattg ccttctcttt taccgatttt ttttaaatac gtttcccatt tagccgtcat
      181 ttcagcactc gttaaaaggt gctgactttc aactgcctgg cacaataatt ttcctttttc
      241 agttacaaca agcttattct taatcacttg gatatattct ttttgtttta acgcttcaat
      301 aatacttgct cttgtcgctt ctgttccaat cccctcaact tcttgtaaaa tcttgattgc
      361 ttcttcatca tcaaccgttt tattcgccgt tttcatagca gttaataatg taccctctgt
      421 aaaagctttt ggcggttgcg tttctttttc aacactctta acaccaactt cggcacgttc
      481 gccaatgata acgagtggta acgtttgaac gtcctctttt tcttctttgg tttgttgttt
      541 gaaaagaatt ttccaccctt cttgctttgg tgtctttccg atcgattgaa acagcaaatc
      601 agctactttc gtttgtatct tggtttcttc atacaagtaa tcaggtaaaa acatggcaac
      661 ggtcgtttta acgactaaag cataaatttt tcgttgtaaa tcgtccattt tcgctaatgc
      721 agattcggta ggtacttgtt ttgttgggat aatggcgtgg tgttcctgta ccttactacc
      781 gtccacataa cgctttctag gctctgtttg aaccatttca agatcaagtc ctaaaaaacc
      841 gctatatttg ccaaaattcg cttttaaata agcaaattcg ttctctgtaa taaatggtgt
      901 atctgttctt ggataactca ataattttgc ttcatacagt ccttgcatag cttttaaacg
      961 taaaattttg cgttctaaga cgttttaaat atatctgcgt atatttttat gtgttttatc
     1021 ttttcgtcgg caaatgcgtt aaaatttgcg aaataaaata gctcaagggc gaacgcagtg
     1081 agtgcattta cccttgatct atttttttga gtaaattagc ttttgaaacc gacggaaagg
     1141 aaacacaaaa cttttcagag gcaaggggtt tttaaggggc gcttttagcc ccttgaattt
     1201 atttgcttta agatatattt agttttaaaa tagttttaac gaaaaaaaga cataaaaaaa
     1261 gacgagcaac gctaccaacg ttctcgcccg gaataacaaa ccacacttgt tattcattcg
     1321 atgattagta tatcaatttt tgattttgaa tacaaaacaa aatggacgaa tggatacagg
     1381 cgaaatttat tttgcgattc cactcgtcct tttttcgttc caaaaattat tttaggagcg
     1441 ataaaaaatg aagaaaattg atgaaaaatt agctgcagaa atctcactaa aaaaaccggc
     1501 cgtctctaat agccggttaa gtaatagccg gttaagtggt caaactttgg gaaaatctca
     1561 acccgcatta agttttgatg ccatgacaat cgttggaaat ttgaacaaaa ctaatgctaa
     1621 aaagctatct gactttatga gtgtagagcc acaaatacga ctttgggata tacttcaaac
     1681 aaagtttaaa gctaaggcac ttcaagaaaa agtttatatc gaatatgaca aagtaaaagc
     1741 agatacttgg gatagacgta atatgcgtgt tgaatttaat cccaataaac tcacacatga
     1801 agaaatgatt tggttaaaac aaaatattat cgactacatg gaagatgacg gttttacaag
     1861 attagactta gcttttgatt ttgaagatga tttgagcgat tactatgcaa tgactgataa
     1921 agcagttaag aaaactgttt tttatggtcg taatggcaag ccagaaacaa aatattttgg
     1981 tgtccgtgat agtgatagat ttattagaat ttataataaa aaacaagaac gtaaagataa
     2041 cgcagatgtt gaagttgtgt ctgaacattt atggcgtgta gaagttgaat taaaaagaga
     2101 tatggttgat tactggaatg attgttttaa tgatttacac atcttgaaac ctgcgtgggc
     2161 tactttagaa aaaattaatg agcaagctat ggtttatact ttgttgcatg aagaaagtat
     2221 gtggggaaag ctaagtaaga atactaagac taaatttaaa aaattgatta gagaaatatc
     2281 tccaattgat ttaacggaat taatgaaatc gactttaaaa gcgaacgaaa aacaattgca
     2341 aaagcagatt gatttttggc aacgtgaatt taggttttgg aagtaaaata agttttattt
     2401 gataaaaatt gctaattcag tataattaat atttacgagg tgacataacg tatgaaaaaa
     2461 tcagaggatt attcctccta aatataaaaa tttaaaattt aggaggaagt tatatatgac
     2521 ttttaatatt attgaattag aaaattggga tagaaaagaa tattttgaac actattttaa
     2581 tcagcaaact acttatagca ttactaaaga aattgatatt actttgttta aagatatgat
     2641 aaaaaagaaa ggatatgaaa tttatccctc tttaatttat gcaattatgg aagttgtaaa
     2701 taaaaataaa gtgtttagaa caggaattaa tagtgagaat aaattaggtt attgggataa
     2761 gttaaatcct ttgtatacag tttttaataa gcaaactgaa aaatttacta acatttggac
     2821 tgaatctgat aaaaacttca tttcttttta taataattat aaaaatgact tgcttgaata
     2881 taaagataaa gaagaaatgt ttcctaaaaa accgatacct gaaaacacca taccgatttc
     2941 aatgattcct tggattgatt ttagttcatt taatttaaat attggtaaca atagcagctt
     3001 tttattgcct attattacga taggtaaatt ttatagtgag aataataaaa tttatatacc
     3061 agttgctctg caacttcatc attctgtatg tgatggttac catgcttcac tatttatgaa
     3121 tgaatttcaa gatataattc atagggtaga tgattggatt tagtttttag attttgaaag
     3181 tgaatttaat tttatacacg taagtgatca taaaatttat gaacgtataa caaccacatt
     3241 ttttggttgc ttgtggtttt gattttgaat ttggttttga acttatggac tgatttattc
     3301 agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga gcacacgcat taatgactta
     3361 tgaaacgtag taaataagtc tagtgtgtta tactttactt ggaagatgca ccgaataaaa
     3421 aatattgaag aacaactagc aaaagatttt aaagagttat tttattttaa gtctttataa
     3481 catgagtgaa gcgaattttt aaatttcgat agaaattttt acatcaaaaa gccccctgtc
     3541 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
     3601 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
     3661 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
     3721 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
     3781 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
     3841 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
     3901 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
     3961 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
     4021 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
     4081 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
     4141 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
     4201 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
     4261 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
     4321 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
     4381 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
     4441 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
     4501 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     4561 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     4621 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     4681 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     4741 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     4801 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     4861 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     4921 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     4981 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     5041 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     5101 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     5161 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     5221 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     5281 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     5341 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     5401 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     5461 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     5521 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     5581 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     5641 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     5701 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     5761 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     5821 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     5881 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     5941 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     6001 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     6061 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     6121 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     6181 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     6241 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     6301 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     6361 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     6421 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     6481 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     6541 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     6601 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     6661 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     6721 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     6781 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     6841 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     6901 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     6961 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     7021 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     7081 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     7141 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     7201 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     7261 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     7321 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     7381 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     7441 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     7501 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     7561 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     7621 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     7681 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     7741 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     7801 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     7861 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     7921 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     7981 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     8041 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     8101 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     8161 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     8221 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     8281 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     8341 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     8401 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     8461 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     8521 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     8581 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     8641 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     8701 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     8761 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     8821 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     8881 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     8941 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     9001 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     9061 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     9121 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     9181 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     9241 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     9301 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     9361 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     9421 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     9481 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     9541 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     9601 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     9661 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     9721 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     9781 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     9841 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     9901 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     9961 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
    10021 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
    10081 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
    10141 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
    10201 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
    10261 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
    10321 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
    10381 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
    10441 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
    10501 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
    10561 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
    10621 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
    10681 tttaacgagg ctgaactgcc tgttgacaat g
//
LOCUS       DASJPH010000098        10668 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJPH010000098 DASJPH010000000
VERSION     DASJPH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.099"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..311
                     /locus_tag="U3995_002353"
     CDS             <1..311
                     /locus_tag="U3995_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HEN1797648.1"
                     /translation="AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRL
                     QNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD
                     "
     gene            346..1113
                     /locus_tag="U3995_002354"
     CDS             346..1113
                     /locus_tag="U3995_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1797649.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1305..1703
                     /locus_tag="U3995_002355"
     CDS             1305..1703
                     /locus_tag="U3995_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1797650.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2010..2384)
                     /locus_tag="U3995_002356"
     CDS             complement(2010..2384)
                     /locus_tag="U3995_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797651.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2406..2783)
                     /locus_tag="U3995_002357"
     CDS             complement(2406..2783)
                     /locus_tag="U3995_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797652.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(2773..3168)
                     /locus_tag="U3995_002358"
     CDS             complement(2773..3168)
                     /locus_tag="U3995_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1797653.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3235..3420)
                     /locus_tag="U3995_002359"
     CDS             complement(3235..3420)
                     /locus_tag="U3995_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN1797654.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3502..3756)
                     /locus_tag="U3995_002360"
     CDS             complement(3502..3756)
                     /locus_tag="U3995_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797655.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3778..4023)
                     /locus_tag="U3995_002361"
     CDS             complement(3778..4023)
                     /locus_tag="U3995_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797656.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4030..4314)
                     /locus_tag="U3995_002362"
     CDS             complement(4030..4314)
                     /locus_tag="U3995_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797657.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4417..4599)
                     /locus_tag="U3995_002363"
     CDS             complement(4417..4599)
                     /locus_tag="U3995_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1797658.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(4805..5344)
                     /locus_tag="U3995_002364"
     CDS             complement(4805..5344)
                     /locus_tag="U3995_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797659.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5457..5723)
                     /locus_tag="U3995_002365"
     CDS             complement(5457..5723)
                     /locus_tag="U3995_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1797660.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5725..5946)
                     /locus_tag="U3995_002366"
     CDS             complement(5725..5946)
                     /locus_tag="U3995_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEN1797661.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6172..6990
                     /locus_tag="U3995_002367"
     CDS             6172..6990
                     /locus_tag="U3995_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1797662.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            6994..7290
                     /locus_tag="U3995_002368"
     CDS             6994..7290
                     /locus_tag="U3995_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797663.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7472..7825)
                     /locus_tag="U3995_002369"
     CDS             complement(7472..7825)
                     /locus_tag="U3995_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797664.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(7845..8900)
                     /locus_tag="U3995_002370"
     CDS             complement(7845..8900)
                     /locus_tag="U3995_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HEN1797665.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9116..9514
                     /locus_tag="U3995_002371"
     CDS             9116..9514
                     /locus_tag="U3995_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797666.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9517..9690
                     /locus_tag="U3995_002372"
     CDS             9517..9690
                     /locus_tag="U3995_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797667.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3542 a         1636 c         1819 g         3671 t
ORIGIN      
        1 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
       61 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
      121 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
      181 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
      241 taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct tatgtgaacc
      301 tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa
      361 ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga tttaaagtcg
      421 tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa gaaaacaggc
      481 gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca aaatcttgaa
      541 acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga aatttcgaat
      601 ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat tcattgggat
      661 ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt aattgtctca
      721 atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca aaaaaaatat
      781 agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat cgccaaaaat
      841 aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat agttaaaatt
      901 cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga tagacgtgtt
      961 ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga ttttgctaaa
     1021 gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag tgagtttgat
     1081 gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac tcgtttgaag
     1141 atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta atctccttta
     1201 gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct tttttttgaa
     1261 acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa tacttttttt
     1321 gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat tttccagact
     1381 tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa gtattggaag
     1441 gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag
     1501 agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta aaagctaata
     1561 cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg actgtggaac
     1621 actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct ttgaatgatt
     1681 tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa aaacagcttg
     1741 ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa aagctctcag
     1801 aagccttctg agagctttta aattgctatg tattgtactg catctgctca aattgatttt
     1861 cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata tgtattgagt
     1921 aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat gtgttaggat
     1981 aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     2041 ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat ccctgcatcg
     2101 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     2161 tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga cttataaaaa
     2221 agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta tttccaaaga
     2281 tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc tgctaattga
     2341 tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt
     2401 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     2461 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     2521 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     2581 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     2641 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     2701 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     2761 tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa gtagttgaga
     2821 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     2881 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     2941 taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     3001 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     3061 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     3121 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     3181 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     3241 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     3301 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     3361 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     3421 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     3481 agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag caaattgaat
     3541 gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat aggtatctaa
     3601 gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt tctccaagtc
     3661 ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta tgaaaacata
     3721 ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt tcttgtacta
     3781 agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata ggacgtgacc
     3841 gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa ggctagtacc
     3901 tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg gatcaatgcg
     3961 agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt ccaaagtcca
     4021 cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt caatagtcac
     4081 ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca agttcccgat
     4141 gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca cttgccgact
     4201 gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg cattaattgt
     4261 ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta gcatcttgtc
     4321 actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc ggtgtgtata
     4381 atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa acgataaagt
     4441 aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg ctttgcttgt
     4501 gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc cagtaaagaa
     4561 taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc cttctgtaat
     4621 attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat ggtgatgttt
     4681 ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt acctaatacg
     4741 tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt tatgtattta
     4801 agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga gtgagtgatc
     4861 catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca gcgacatacg
     4921 atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt tttggtttat
     4981 tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga tcaccgacaa
     5041 agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca tttaagaagt
     5101 agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag gttccttctg
     5161 catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg atttccaaac
     5221 catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa taatcaccat
     5281 accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca cttatattat
     5341 acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa gtgggatcgt
     5401 aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct tgtctgttac
     5461 ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc atgctcgcta
     5521 atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga tttattagct
     5581 gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt ctcgatccag
     5641 tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg agcttcacgt
     5701 tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat cttcgtggga
     5761 aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg caaagtctac
     5821 taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt tatctccgtt
     5881 gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa tacttattac
     5941 tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg acaaatgcaa
     6001 tagttatata tttgaatata tacataaaca taaacatata catatatatg tttatgttgt
     6061 agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata tacataaacg
     6121 tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat catggaaaag
     6181 aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac aacaacaatc
     6241 tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa
     6301 gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc gaaaagagag
     6361 aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat aaatgagaac
     6421 atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat gcgtgatatg
     6481 cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca gcttaaagag
     6541 aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc tcaaaatgct
     6601 tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg ttttgaagct
     6661 ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga tattaagact
     6721 ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa acataatact
     6781 aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct tggatatgta
     6841 caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc tgagttcgct
     6901 aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt tacgctatac
     6961 aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga aaatagattt
     7021 aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc agttgaagaa
     7081 cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa aagtgatagt
     7141 tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa gaaattatct
     7201 aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga aatgatagat
     7261 aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact ttttttattg
     7321 gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg atatatacgt
     7381 atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac ataataaaaa
     7441 ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta cttggtgcat
     7501 tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta accgttgaag
     7561 gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct gctactttgc
     7621 tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt acacgccgat
     7681 ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt tcatattcgg
     7741 attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt ataactacat
     7801 aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg agttaaacca
     7861 aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa ggtatgcgga
     7921 attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt aaagacaact
     7981 ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac catattggtg
     8041 tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt tccgcttggc
     8101 ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag ttggaaagca
     8161 ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt aaaaatgccg
     8221 gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac atcaaaaagt
     8281 ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt
     8341 acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg tggcagtacc
     8401 caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga atgttgtcgt
     8461 gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga aggcagacct
     8521 aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc taggttagct
     8581 aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat cccaaaacct
     8641 agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc aaaataatag
     8701 ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt taatgaggaa
     8761 cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg agagggaagt
     8821 gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa gtcaacggaa
     8881 aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg tatttcggta
     8941 cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa aaaatgcaat
     9001 cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata aatgcttgca
     9061 aatttatctt attatgatat tataaaatca taaattatat taaggagtga tcctgatgcg
     9121 attgagaaga aataagaatt acactaccat tattttagaa gattttactg atcaagaatt
     9181 acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt caatagtaaa
     9241 agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg ctaaggaaat
     9301 ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta gcttgatccc
     9361 aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg aaaaacatac
     9421 ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat tggatgtaaa
     9481 atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt tagaaaatta
     9541 ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc agggtttaag
     9601 ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt tttatctggc
     9661 ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt agtaaattaa
     9721 acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat cagcttgtaa
     9781 taagattata aaataacata atatcaaaaa tagtgctttt taaaggacaa attaagtgta
     9841 tcaacataaa cgtgtatatg tatatgttta tgttgatact ttttttgtct taatttttta
     9901 tttttaaaat tctatttact aatgctgtat agatagtgat cagaaaaaac aaaatagcga
     9961 gattaagcta tttattagtt catatattat attcagatta atatgatcca gtattaatct
    10021 gaaggcaaaa ccaatatatt tctacggact cttgtaaaac tatttaaatt ttcctgctaa
    10081 taaaataacg aaaatagaat atttagcttt tttataccat tttacgataa catgatttca
    10141 tttgtaatat taaaacattt aaatttttaa ttattctttc aaattagatc aattaatatc
    10201 cttataaaag agcataataa atggttttta catcgttatt tattgatatt aatctcatcc
    10261 aaaaaaaatg aaaacgcata cttttatggg tgattgaatg tgattttaat acatatacca
    10321 attaaaagaa aactttttag atttttactt acaaatttat aaaaaaacaa gataattgat
    10381 actttgattt tgcgaacatt ttatcttgcc gcctaattca gctttaatat catgattata
    10441 tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct taatcaggga
    10501 aaaaataact aataaatgga ttttgggaat aggtaaaaac ttgatcggtt tctggctaaa
    10561 aataacgtga aaaaggaaat ataaaataac atggagcgtg ggaggaataa agaaatttta
    10621 cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa acaaaagt
//
LOCUS       DASJPH010000099        10662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJPH010000099 DASJPH010000000
VERSION     DASJPH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.100"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..1498)
                     /gene="cls"
                     /locus_tag="U3995_002373"
     CDS             complement(47..1498)
                     /gene="cls"
                     /locus_tag="U3995_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1797668.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1839..4505
                     /locus_tag="U3995_002374"
     CDS             1839..4505
                     /locus_tag="U3995_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1797669.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4869..5621
                     /locus_tag="U3995_002375"
     CDS             4869..5621
                     /locus_tag="U3995_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1797670.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5643..6797)
                     /locus_tag="U3995_002376"
     CDS             complement(5643..6797)
                     /locus_tag="U3995_002376"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1797671.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            6982..9423
                     /locus_tag="U3995_002377"
     CDS             6982..9423
                     /locus_tag="U3995_002377"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1797672.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9580..>10662)
                     /locus_tag="U3995_002378"
     CDS             complement(9580..>10662)
                     /locus_tag="U3995_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HEN1797673.1"
                     /translation="TQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTK
                     ELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFK
                     SMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNAS
                     YCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYW
                     KLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     DTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3392 a         1934 c         2276 g         3060 t
ORIGIN      
        1 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
       61 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
      121 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
      181 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
      241 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
      301 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
      361 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
      421 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
      481 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
      541 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
      601 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
      661 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
      721 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
      781 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
      841 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa
      901 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat
      961 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     1021 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     1081 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     1141 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     1201 caacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     1261 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     1321 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     1381 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     1441 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     1501 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     1561 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     1621 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     1681 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     1741 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga
     1801 caggactagg gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac
     1861 aacaagcggt ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg
     1921 atggattgag caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa
     1981 aagaaaagcc gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg
     2041 ttatcgtatt gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct
     2101 tagttatttt tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag
     2161 cagaaggttc tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg
     2221 atggtaagga aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag
     2281 acgcaggcga ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag
     2341 atgaaggtat gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa
     2401 gtacggtacc aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg
     2461 gacgcggtaa attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg
     2521 gccttttgga atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca
     2581 gtaaaaaact aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt
     2641 ttcgtattta tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga
     2701 tgtttgccgt agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa
     2761 tcgtgttatc tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc
     2821 cagcagttga aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga
     2881 cacaaaacaa aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca
     2941 caaatgaccc ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag
     3001 cgaatgatgc atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt
     3061 ttatcgactt tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa
     3121 gacaagcaga acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg
     3181 ataacaaaaa ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa
     3241 aagtattgct aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag
     3301 agcaaaatga agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac
     3361 ttgataattt tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg
     3421 ctatgatcga tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag
     3481 gaatcaaaac aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac
     3541 aaatcgggat ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga
     3601 cagaagatca actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg
     3661 agaataaaat tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag
     3721 gtgatggtgt aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa
     3781 cgggtacaga tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt
     3841 ctattgtcag cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt
     3901 cttatctatt tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg
     3961 actggtccaa tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac
     4021 tgccagcaat tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa
     4081 gagatccaaa ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg
     4141 tgctgattgg gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat
     4201 tcggtgtagc catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag
     4261 cacgttcaaa cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac
     4321 tagctgtagt tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag
     4381 tcttctctat cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac
     4441 ttgtgacaat tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata
     4501 aataaaacga atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat
     4561 ttcaacaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag
     4621 gagaacaccg tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca
     4681 tctgattgga cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc
     4741 tttttttatt tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa
     4801 tagataaaag aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag
     4861 gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc
     4921 aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc
     4981 tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa
     5041 aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa
     5101 aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat
     5161 ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac
     5221 atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag
     5281 tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag
     5341 aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag
     5401 gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat
     5461 tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca
     5521 aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc
     5581 tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt
     5641 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
     5701 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
     5761 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
     5821 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
     5881 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
     5941 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
     6001 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
     6061 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
     6121 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
     6181 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
     6241 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
     6301 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
     6361 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
     6421 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
     6481 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
     6541 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
     6601 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     6661 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     6721 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     6781 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     6841 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     6901 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     6961 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     7021 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     7081 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     7141 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     7201 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     7261 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     7321 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     7381 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     7441 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     7501 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     7561 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     7621 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     7681 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     7741 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     7801 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     7861 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     7921 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     7981 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     8041 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     8101 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     8161 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     8221 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     8281 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     8341 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     8401 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     8461 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     8521 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     8581 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     8641 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     8701 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     8761 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     8821 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     8881 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     8941 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     9001 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     9061 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     9121 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     9181 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     9241 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     9301 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     9361 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     9421 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     9481 atcttgcttt tttggctctt cgtcaaattg tgttggcgaa gacaaatttg aataaaactt
     9541 agtccgatat gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa agatcagacc
     9601 ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg cgataaccaa acgcaattcg
     9661 tttaatcagc ttgatcttgt tattcagccc ctcagtgacg ccgtttgagt ggggcacctt
     9721 aaaggcattt gaaatacccg attggtagcg atggaagatc gtgaatttct ttttaaacga
     9781 ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga aagccttccg tatctcgttt
     9841 aacgaaataa tagcgtaata attgacaaaa atgatacgca gttcgcaatt cttcgttgta
     9901 gcttaacagc tcatcaacga tttctgtttg actaattggt cgtttaaaca gtcgatgata
     9961 atgccgtttg agtggctcca atgtcgtgga atctttgagt aagagtttcc aatatcgctt
    10021 gagccgacgg tacttcttct gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg
    10081 gacacgtaat tgattgaagc tgcgattgat gtgttggaca atatgaaagc ggtcagttac
    10141 gatctccgca tttggaaaga ctgttttaag caactgacaa tagctggcat tcatgtccat
    10201 cactaggtac ttgaccttta aacgggcctt tctaggaaag cgcaggaagt attgagttag
    10261 tttgtaaagt ctgcgatctt caaggatcgt aaatagctga ttggttgtcc catcaacaca
    10321 aataaaactc atggaaccag aaacagactt cattgatttg aactcatcca tacacaacac
    10381 cgtcggaagg aagttcatac gtggctggta atgcttcgtt gcttcacgca agatccgtgt
    10441 caccgtccca tccgagacga agtacttctt agcgattagt ttacgtgatt gattttcgta
    10501 cagttccatg agaatcgcct gcttcagttc ctttgttagt tgatgatgtt catcaactaa
    10561 gtccgttttc gcagaaaagg tccgtccaca atcatggcag agaaaacggg tacgttttaa
    10621 ttctaaaata gtcaactttt ctttgacttt caacatctga gt
//
LOCUS       DASJPH010000100        10515 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJPH010000100 DASJPH010000000
VERSION     DASJPH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.101"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..305)
                     /locus_tag="U3995_002379"
     CDS             complement(132..305)
                     /locus_tag="U3995_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1797674.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            753..3115
                     /locus_tag="U3995_002380"
                     /pseudo
     CDS             753..3115
                     /locus_tag="U3995_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3483..4406
                     /gene="truB"
                     /locus_tag="U3995_002381"
     CDS             3483..4406
                     /gene="truB"
                     /locus_tag="U3995_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN1797675.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4410..5351
                     /gene="ribF"
                     /locus_tag="U3995_002382"
     CDS             4410..5351
                     /gene="ribF"
                     /locus_tag="U3995_002382"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1797676.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5703..6488
                     /locus_tag="U3995_002383"
     CDS             5703..6488
                     /locus_tag="U3995_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1797677.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6501..7352
                     /locus_tag="U3995_002384"
     CDS             6501..7352
                     /locus_tag="U3995_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1797678.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7476..7928)
                     /locus_tag="U3995_002385"
     CDS             complement(7476..7928)
                     /locus_tag="U3995_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797679.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8393..8761)
                     /locus_tag="U3995_002386"
     CDS             complement(8393..8761)
                     /locus_tag="U3995_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1797680.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8911..9393
                     /locus_tag="U3995_002387"
     CDS             8911..9393
                     /locus_tag="U3995_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1797681.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9430..10440
                     /locus_tag="U3995_002388"
     CDS             9430..10440
                     /locus_tag="U3995_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1797682.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3574 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag agttccatga
       61 ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta aaaaattcga
      121 ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa gataccgata
      181 acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga
      241 tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa
      301 atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat
      361 tttggataat ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa
      421 agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc
      481 ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat gcatgatgag
      541 gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa
      601 aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat
      661 aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag
      721 aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg
      781 ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta
      841 aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa catccaatcg
      901 gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca
      961 ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga caagtcatgg
     1021 tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg
     1081 agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt gcttctcatg
     1141 cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac
     1201 atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg
     1261 gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag
     1321 tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa
     1381 cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg
     1441 aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg gctgaaaaaa
     1501 tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg aagacagcaa
     1561 cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg
     1621 gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga
     1681 ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct
     1741 atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaatcg
     1801 cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa
     1861 gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg
     1921 ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc
     1981 cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt
     2041 tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt
     2101 cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag
     2161 aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat tcttgcgagt
     2221 ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg acctttcttg
     2281 gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc agcaagatca
     2341 caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa agaaggcgga
     2401 gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca tggataaagc
     2461 attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc gtgctcaatt
     2521 ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc
     2581 ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa cggaatctaa
     2641 cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac taaaaattcg
     2701 gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt
     2761 gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat ttgctttcca
     2821 cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc atttaatgat
     2881 tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg tagtaaatga
     2941 acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga
     3001 agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata ttcgtgaaaa
     3061 tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc aataaaatga
     3121 taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa aaaataaata
     3181 aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt tgggaagtga
     3241 aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc aaagaaaaaa
     3301 tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta cgtgagcatg
     3361 aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg tcaaattcgt
     3421 cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa tggagaaaag
     3481 acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat gattgcgtat
     3541 tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg cttgacccag
     3601 acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc gaatatttga
     3661 cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca acgacggaag
     3721 atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct gaaaaaaaaa
     3781 tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca atgtactcag
     3841 cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa acagtggagc
     3901 gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg acaagagacg
     3961 aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga acctatgtcc
     4021 gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg tctgatctga
     4081 cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa gaagtagctc
     4141 aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa ggagtggaac
     4201 ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac ggtatgagac
     4261 ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct ttgttttata
     4321 aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa ttaaaaccta
     4381 gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga catccttacc
     4441 aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc gacggcgtgc
     4501 atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa gaaggattga
     4561 aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag atcgatccta
     4621 gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc gctttgggta
     4681 tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca ccgcaggaat
     4741 ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt tttgattata
     4801 cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc aaaggaagat
     4861 tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc tctacccgta
     4921 ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta ggttgcacat
     4981 atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta ggattcccta
     5041 cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt tatgtgtcgg
     5101 aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat aatgatacat
     5161 tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat caagatatct
     5221 atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg aaattttcta
     5281 gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct gaatattttt
     5341 ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc aattacaata
     5401 aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc acagcctttt
     5461 tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa atttcttgaa
     5521 gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact agcaattcat
     5581 gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg tacactcact
     5641 ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa ggagtgggta
     5701 ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca aaggggattg
     5761 gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc aactataact
     5821 cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc ggaaaagcag
     5881 tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt gatacggctt
     5941 tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa aatcagcatc
     6001 caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat ttgactggcg
     6061 tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa aaaggaaata
     6121 tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct cattacgctg
     6181 cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat gcaccgaagg
     6241 gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat gcggcaaaat
     6301 ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga cgcgtaggtg
     6361 aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc agttatgtta
     6421 caggtattac actatttgca gatggtggga tgactttata tccttctttc caaggtggaa
     6481 aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca gttggctggg
     6541 ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag atattaggaa
     6601 ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct ccagctggta
     6661 tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt attggacaaa
     6721 caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca atttcgactg
     6781 cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa tggtctggaa
     6841 gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt tctgctttaa
     6901 cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt agtctattag
     6961 catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt gatgcaagcg
     7021 gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg atttatgtga
     7081 ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg atcgttggag
     7141 ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat ggggttgcta
     7201 ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt atgtacatcc
     7261 tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta ttgctgatcg
     7321 tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat ttcatttatt
     7381 aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg ctagaatttc
     7441 caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga aatatctgtc
     7501 tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt gaagttacca
     7561 ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag acggtctttt
     7621 ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg attgattgct
     7681 gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag catgaatcgc
     7741 tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa aagattacgt
     7801 tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc atgaagataa
     7861 tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac tgagtaaaaa
     7921 tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat atttgcttaa
     7981 tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa gtcagaaaat
     8041 tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta tatttaaata
     8101 tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt attttttcct
     8161 tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta gaaaattcaa
     8221 agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa atagcagata
     8281 ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa caaaacctgt
     8341 ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag ctttatcgcc
     8401 aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt tcaatcaatt
     8461 cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat acgtagcctg
     8521 cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat
     8581 gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac ataggttggt
     8641 tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca aaaaggtctt
     8701 tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga aatttttcca
     8761 ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa tagttatgta
     8821 taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga taagatgtct
     8881 caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa attcttacac
     8941 gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga agatgctttg
     9001 gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt cttaaaagat
     9061 aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga tttcaaagca
     9121 ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat ggaagaacaa
     9181 acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat tccgattatt
     9241 attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag accagatcaa
     9301 acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga gatgccgata
     9361 tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca tacttggagg
     9421 tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg ggttactgca
     9481 ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt aaagagaatg
     9541 gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga taaggaaaaa
     9601 tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc
     9661 caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga ttttgtcgat
     9721 tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac gttgctactt
     9781 gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat tgatttaaga
     9841 ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac aagagaagga
     9901 gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca tgagcattca
     9961 tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag cggagatgca
    10021 gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca tttaatcggt
    10081 actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa gccgatcttg
    10141 tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact tgttcagatg
    10201 aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag tgcggcagag
    10261 gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga attaaaacta
    10321 ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt gattaatggt
    10381 gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac gtttatttaa
    10441 aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag aaaagaaagt
    10501 gaaagaagct gacaa
//
LOCUS       DASJPH010000101        10503 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJPH010000101 DASJPH010000000
VERSION     DASJPH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.102"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(288..1703)
                     /locus_tag="U3995_002389"
     CDS             complement(288..1703)
                     /locus_tag="U3995_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1797683.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(1720..3036)
                     /locus_tag="U3995_002390"
     CDS             complement(1720..3036)
                     /locus_tag="U3995_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797684.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(3186..3884)
                     /locus_tag="U3995_002391"
     CDS             complement(3186..3884)
                     /locus_tag="U3995_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1797685.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(4173..5129)
                     /locus_tag="U3995_002392"
     CDS             complement(4173..5129)
                     /locus_tag="U3995_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1797686.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(5242..6075)
                     /gene="msrA"
                     /locus_tag="U3995_002393"
     CDS             complement(5242..6075)
                     /gene="msrA"
                     /locus_tag="U3995_002393"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1797687.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(6250..7884)
                     /locus_tag="U3995_002394"
     CDS             complement(6250..7884)
                     /locus_tag="U3995_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797688.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(7871..9739)
                     /locus_tag="U3995_002395"
     CDS             complement(7871..9739)
                     /locus_tag="U3995_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797689.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            9705..>10503
                     /locus_tag="U3995_002396"
     CDS             9705..>10503
                     /locus_tag="U3995_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797690.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTH"
BASE COUNT         3268 a         2019 c         1616 g         3600 t
ORIGIN      
        1 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
       61 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
      121 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
      181 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
      241 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
      301 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
      361 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
      421 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
      481 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
      541 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
      601 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
      661 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
      721 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
      781 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
      841 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
      901 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
      961 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
     1021 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
     1081 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
     1141 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
     1201 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
     1261 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
     1321 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
     1381 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
     1441 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
     1501 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
     1561 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
     1621 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
     1681 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
     1741 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
     1801 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
     1861 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
     1921 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
     1981 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
     2041 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
     2101 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
     2161 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
     2221 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
     2281 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
     2341 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
     2401 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
     2461 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
     2521 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
     2581 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
     2641 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
     2701 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
     2761 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
     2821 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
     2881 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
     2941 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
     3001 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
     3061 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
     3121 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
     3181 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
     3241 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
     3301 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
     3361 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
     3421 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
     3481 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
     3541 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
     3601 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
     3661 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
     3721 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
     3781 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
     3841 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
     3901 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
     3961 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
     4021 aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat
     4081 atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta
     4141 atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat
     4201 tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt
     4261 aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg
     4321 gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt
     4381 tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta
     4441 gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg
     4501 aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt
     4561 gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt
     4621 aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat
     4681 gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca
     4741 gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct ttggagactt
     4801 tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac
     4861 atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct
     4921 ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca
     4981 aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg
     5041 ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa
     5101 cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac
     5161 aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta
     5221 ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt
     5281 gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg
     5341 tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc
     5401 tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt
     5461 tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg
     5521 cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat
     5581 tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac
     5641 atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt
     5701 gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca
     5761 gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt
     5821 ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat
     5881 tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga
     5941 taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt
     6001 tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat
     6061 ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg
     6121 taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat
     6181 aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga
     6241 aaagttaatt taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa
     6301 tgtaacaaga taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc
     6361 aactcctttg actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg
     6421 ttgatgattt atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg
     6481 gctcttctca ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt
     6541 tacccattga ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga
     6601 agcgatagga gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac
     6661 tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt
     6721 tttactagaa acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc
     6781 tcttgtctta ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa
     6841 tattatttgc aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg
     6901 cagaagatac catggcacca aactagttgt agctagccaa gtcaaacacg taccataata
     6961 aatctttttt tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa
     7021 cagaccaact agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact
     7081 atcgaacaca ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg
     7141 gtagagcatt tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc
     7201 tgccgcacta aataattttt taaatatagc aataaaatct gcattcttga aacctttaga
     7261 aaccaaacga ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac
     7321 atggctgtac actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc
     7381 ttttccagag atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat
     7441 agcttcggca attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata
     7501 gagacaacta aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata
     7561 agctacgagt gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa
     7621 attttttgtc atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata
     7681 atttacacca tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt
     7741 cgataacccc ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg
     7801 tgtatttttg aaaaagatga aagaatatat actactaact ataattaatc cactcaatat
     7861 tactactttt ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag
     7921 aatctttcaa cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag
     7981 tcagaatact gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta
     8041 tcttattttc tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat
     8101 ttacgacttt accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca
     8161 ctttttgctt tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact
     8221 tccctttttc ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt
     8281 ttatcgcttc tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg
     8341 ggagtgtttc ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat
     8401 tttcttttgc tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc
     8461 atgaatattt tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa
     8521 aaaagatttg gatacctaag atacgccaag gaaactggag taagcttatc ggtgtttttt
     8581 gaaacaaaaa ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat
     8641 agatgatttt ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa
     8701 ccaatcctaa taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac
     8761 tatctgaaat agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt
     8821 gataattcat ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa
     8881 aaacagcctt tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta
     8941 aaaacaaacg aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga
     9001 catgagtata cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat
     9061 tgaatatcaa tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt
     9121 ctgcaattgc ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac
     9181 aactaaataa aaaagcagca tctatctttt taaaaaactt ttctccataa taaaaactaa
     9241 tcagaatcgt acaaatattt aataaccaaa tatatagaac ataacttaat attaagttat
     9301 ttgaaattcc ataaaaaacg actgcaggaa ttattgtaag aaaaggataa aaatagttca
     9361 gtccatctcc ataattgtta aatgttgtat agttgattgg cccttcaaaa atagaagata
     9421 accctttgat tcgtgaaaga tgaaaagata aatcacctgt tgtataaatt tcttgagcat
     9481 ttctcataaa atttactgaa tataaagtac taagtaagat aagaactact aaaagaaatg
     9541 cttttttact tattttcata gatttgttgt tctccttttt taattctatt ttcagtatac
     9601 tagaattgtc tttttttaat aagttttgcg ttaaattttt catagcacta gtatgatata
     9661 ctagaaaaga atcaattttt attactttta aataggagaa aaaaatgaaa aaaaacaaaa
     9721 accaaataat agatatcatt tttatgttat ttctaagttt tgcttacgtc atccctctat
     9781 tcatgtctaa gggaatctat catagtgtca atcaggatac ttactttcat ttatctcgaa
     9841 tcataggact agataatgta tggagttctc cagtaaactt caataacttc gatcatcatg
     9901 gaacaatgat gaatattttt tatccatggc ttactcttta tccagctttt ttattttata
     9961 aaatgatggg aaatcttgtt ttaagttata atatctacta tttttttatc acttttttaa
    10021 caatggtcgt ttcttatttt tctatgaaac aaataaaaaa caatcgttat atttcgctgt
    10081 tattttcgat tatttataca ttttccgcct atcgtgccat tgacatattc catcgtgctt
    10141 cattgggaga agctgtcgct ttaacatttt tgcctttgat actaatgggg tgttacgaaa
    10201 tttatattag agattaccaa aaatggtact ggttgtctat tggcatgacg ctagtagtgt
    10261 atactcattt actttctgtc gctatggttt ctgtatttat tggaggaacg ttgtttcttt
    10321 ctttttattt ttgggaccaa aaaatcgcta gactcttaag tcttttaaaa gctaccgtcc
    10381 tcaccttttt tttaagtgcg ggattcttaa ttccttttat tcaacaatct agagcacaag
    10441 aattgaaagt cccactggga aaagaattaa gtggtatggc tcctagtgat atgctgacac
    10501 ata
//
LOCUS       DASJPH010000102         9950 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJPH010000102 DASJPH010000000
VERSION     DASJPH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.103"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1557)
                     /locus_tag="U3995_002397"
     CDS             complement(46..1557)
                     /locus_tag="U3995_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1797691.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /locus_tag="U3995_002398"
     CDS             complement(1879..3231)
                     /locus_tag="U3995_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1797692.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="U3995_002399"
     CDS             complement(3384..4460)
                     /locus_tag="U3995_002399"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1797693.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="U3995_002400"
     CDS             4656..6401
                     /locus_tag="U3995_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1797694.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="U3995_002401"
     CDS             complement(6681..7118)
                     /locus_tag="U3995_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1797695.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="U3995_002402"
     CDS             complement(7102..8475)
                     /locus_tag="U3995_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797696.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="U3995_002403"
     CDS             complement(8797..9285)
                     /locus_tag="U3995_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797697.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="U3995_002404"
     CDS             complement(9307..9909)
                     /locus_tag="U3995_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1797698.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2913 a         2119 c         1798 g         3120 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc
//
LOCUS       DASJPH010000103         9811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJPH010000103 DASJPH010000000
VERSION     DASJPH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.104"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="U3995_002405"
     CDS             complement(34..2154)
                     /locus_tag="U3995_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1797699.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="U3995_002406"
     CDS             2384..3562
                     /locus_tag="U3995_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1797700.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="U3995_002407"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="U3995_002407"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1797701.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="U3995_002408"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="U3995_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1797702.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="U3995_002409"
     CDS             complement(4915..6168)
                     /locus_tag="U3995_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1797703.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="U3995_002410"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="U3995_002410"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1797704.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="U3995_002411"
     CDS             7746..9092
                     /locus_tag="U3995_002411"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1797705.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="U3995_002412"
     CDS             complement(9400..9786)
                     /locus_tag="U3995_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1797706.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DASJPH010000104         9681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJPH010000104 DASJPH010000000
VERSION     DASJPH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.105"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="U3995_002413"
     CDS             51..260
                     /locus_tag="U3995_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797707.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="U3995_002414"
                     /pseudo
     CDS             467..667
                     /locus_tag="U3995_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="U3995_002415"
     CDS             complement(1379..1984)
                     /locus_tag="U3995_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1797708.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="U3995_002416"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="U3995_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="U3995_002417"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="U3995_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="U3995_002418"
     CDS             3019..3990
                     /locus_tag="U3995_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1797709.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="U3995_002419"
     CDS             4130..5443
                     /locus_tag="U3995_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1797710.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="U3995_002420"
     CDS             5486..6352
                     /locus_tag="U3995_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1797711.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="U3995_002421"
     CDS             6349..7197
                     /locus_tag="U3995_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1797712.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="U3995_002422"
     CDS             7211..8683
                     /locus_tag="U3995_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1797713.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="U3995_002423"
     CDS             8700..9572
                     /locus_tag="U3995_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1797714.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3037 a         1659 c         2019 g         2966 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa g
//
LOCUS       DASJPH010000105         9570 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJPH010000105 DASJPH010000000
VERSION     DASJPH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.106"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..1222
                     /locus_tag="U3995_002424"
     CDS             101..1222
                     /locus_tag="U3995_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1797715.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1304..3076)
                     /locus_tag="U3995_002425"
     CDS             complement(1304..3076)
                     /locus_tag="U3995_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797716.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3079..4791)
                     /locus_tag="U3995_002426"
     CDS             complement(3079..4791)
                     /locus_tag="U3995_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797717.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4906..5574
                     /locus_tag="U3995_002427"
     CDS             4906..5574
                     /locus_tag="U3995_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1797718.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5641..6429)
                     /locus_tag="U3995_002428"
     CDS             complement(5641..6429)
                     /locus_tag="U3995_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1797719.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6512..7984)
                     /locus_tag="U3995_002429"
     CDS             complement(6512..7984)
                     /locus_tag="U3995_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1797720.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8114..9307)
                     /locus_tag="U3995_002430"
     CDS             complement(8114..9307)
                     /locus_tag="U3995_002430"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1797721.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9305..9384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
BASE COUNT         3096 a         1996 c         1559 g         2919 t
ORIGIN      
        1 gacgatcttt agaattgtca aaagattaaa tatataaaaa gccttcgggc tttttctttt
       61 aacaattaat aggaacgtat attcgaaagg aatagaaata atgtggttag aaaaaataaa
      121 tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa
      181 agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta acttactttt
      241 gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa cttttggaga
      301 attaaaaact aaatgggatg aaaaatacaa gccaactgta aaagaaagtt cctatcgaac
      361 aactcaggtt tatctatcat taatttcaaa atatataaaa gatgatgtac ttgtaaaaaa
      421 cgttaatagt aatcttatac aagatatgct agattactac tattttgaaa aaaatttatc
      481 atataattat gtaatacatt taaaaacttt tacaggaatg atttttaaat tcgccaaccg
      541 tcgatatgga ttaaaatata atcccgtaaa tggggtttct ttagcaagaa aacctaagac
      601 aaacgaagaa ataaaaaaag aaaaagatgg ttatttatca aaagaagaag ttcaaatgat
      661 tgctagtaaa caaaaaagtt cacatcaaca gtcacgttat tctttaatta ctaaattttt
      721 gtttttaact ggactacgtt atggtgaatt gatatctttg actgaatctg attatgatgg
      781 taataaaatt acggtttctg gtacttatga ttacgagtta aaaattaaaa caacaactaa
      841 aaatacaggt tcttatagaa ccattgaatt atcaagtaat gctaaagaaa tcatagatca
      901 attaattgaa gaaaacaaat taattaagaa taataaagat aaatacattt tcatctctaa
      961 aaatggtaac ccgatatcta ttcaagcata taatcaatct ttaagagcag tatccaaaga
     1021 attaaaccta gataaaaaag tttcttctca catgttaagg cattcccaca tttctctact
     1081 tacagaatta ggaatcccat taaaagccat tatggatcgt gtaggacatg aagattcaaa
     1141 aacaacatta aagatttata ctcatacaac taaaaatatg caaaatcaac tagtagaaaa
     1201 actaggaaaa atagaaattt aaattgcccc ttttctgccc cttataaaaa caaacaacat
     1261 aaaatatata aaaagaaccc cgttataacg aggttctttt aatttaactt tcaaatacaa
     1321 attgattatg ataaagttcc gcataaaatc cattttctgc taataactcg tgatgactgc
     1381 cttcttcaat gacttcccca tcacgcaaga caacgattct atctgcattc aagattgttt
     1441 tcagccggtg agcaatcaca aagctggttc ttcctttgat tgcttcatcc attgcttttt
     1501 ggatccttgc ttctgttacc gtgtctacat tacttgttgc ttcatccaaa atcaataaag
     1561 atggatttgt gatgatcgtt cgagcaatac tgatcaactg cttttgacct gtactgaata
     1621 agttattttc ttcggatatc tctgtatcat aacctttttc taacgtcatg atgaagtcat
     1681 gaatattcgc ttgttttgct gcattgatga cttcttcatc tgaagcgtcc ggtttgccga
     1741 atgtgatatt gtctttgatc gttccggaga acaatacaga ttcttgcaaa acgattccta
     1801 catgtctacg taatgcatca agatcataat ctcggatatc tattccatcg atgactactg
     1861 aaccatcatt gacatcgtaa aaacggttta gcagattcat gatcgtcgtt ttccccgatc
     1921 ctgttggacc aacaagagca accatttctc ctttgtccac attgatcgag acatctttca
     1981 agactagttt ttctggagaa taaccaaaat ctacgtgctg caacgcaaga cctttttcaa
     2041 caccatcaaa tgctttacag ttttctggtt tgacttcatc tttttcatca aatatttcat
     2101 tcaatcgtct tgcccctgta atcgctaatt ggatcatgct gtatccagat gaaatctgca
     2161 tcagtggttg gtaatattgt tgtgaatact ggacgaatgt cacaactaag cctaatgcca
     2221 ccgaacgttc caaatcacca tttaatgcta accagccgcc gaaaaagata actatcgctg
     2281 tattgaccag tgacattcct tgcatcatcg gaaatagtag accagaataa gcttgtcctt
     2341 tgaaggttgc ttcgcgaaca ctttgattat gctctaagaa tccttcaatc gtttcttctt
     2401 gtaagccatt tgtgatgatg acacgttgtc cgctgatttt ttcatccata taaccattta
     2461 gtttccccac ttcatcttgc tgaagatcca catatttccg tgctttgcgg ataatcaaga
     2521 cagctatcaa aacggctact ggagtggaag caattgtagc ccaagctaac tctacatttt
     2581 ggcggaacat catgatcaag atcccgacaa gtaaaacagc attcgtgatg acttgaagca
     2641 aagcttggtt caaactattt tgaatattat ctaagtcact ggtaaaacga cttaaaattt
     2701 caccgtcttg atgagaatca aagaaacgga tcgtcaattt ttccaatttg ttgaatagac
     2761 cgatacgcat tcggttcgtt gacttcccta ctacttgtgt gaacaaaata ctatagataa
     2821 agttggctgc actagctagt acataaaaca gcaagagttt ccatattaca gaaacaaatt
     2881 tgcttttatc gtctacgcct tgcatcaagc ctcctacgta attagctaat tcttgaaaag
     2941 cttccccgat gtattgaggt gcctttactt gtagataggt agcaatgatc actgtgataa
     3001 agataaagaa aaaggagagt ttgtaccgct tcaaatagtg atagaaaaat ttacttgctt
     3061 ttattagatc agtcatactt actcctccgt tcctttttga gtttcaaaga tttcctgata
     3121 gacacggttt ccagctacca attcctcatg ggttccttcg ccgactaatc tgccttcatc
     3181 caatactaag atgcggtctg ctttgacaac agaagcaatt ttttgtgcga tgacaatcgt
     3241 cgttgttccc ttcaaatctc gatcgagtgc ttctcggacc agcttttcag agcgagcatc
     3301 cagtgcactc gtactgtcat ctaggatcaa gatctttgga ttaccgataa cgccacgagt
     3361 aatggataaa cgttgctttt gaccaccaga aaagttactg ctacgttctt ctattggtgc
     3421 ttcgtaacga tcagataact tttcgatgaa ttcttttgct tgagcaatag aagaagcatg
     3481 ttccatctct ttttctgttg cattcttttt cccttggcgc aagttttgag caatcgttcc
     3541 tgagaataaa attgcttttt gcaaaacaaa ggaaaccgtt tgtcttaaac tcttttcatt
     3601 tacttgacga agatctacac cacctacttc aactttgcct tctgtcggat caaatagacg
     3661 agggatcaat tgtgctagtg ttgacttccc ggcaccagtc gccccgacga taccaaccat
     3721 ttctccaggt ttgatagaaa agctgatatc tttaagcgta tctgtatcct caccaggata
     3781 acgaaaagaa acatggtcaa aagtgacaga acccgtcaac tcttgttctg gaacgtctaa
     3841 ataagtaaga tctggttcag catctaaaat ttccgcaatc cgcttcagcg atacggctgc
     3901 tcttgaagtc atcatcatca tcattccgcc aataatgatt gccatcataa tctgcatcaa
     3961 gtagttcatg aatgaagcaa ttccaccaat taacgtagga tcatctttaa caagatcgct
     4021 cacaaaaaag atcgccccaa ccacagccaa attggcagcc agcatgaagg acgggatcat
     4081 cacagaaaat aatgtaccga ctatgatgtt gtgttttgtt aagtcctcac ttactttgct
     4141 gaatgacttc aattggtttt cttcttgtac aaaagatttc actacacgaa tccctagtaa
     4201 gttttctttt gcaatgctat tcacacgatc aatcagtttt tgaatgatca tgaagtgctt
     4261 gcccatcctt gtaaatgaaa gtgccgtaat cagaaatacc gtaacgataa gcgctacgat
     4321 gatccaccat aattgcggca tcgtgatcat agctaaaatg aaggcaccaa taaataagaa
     4381 tggaattcga aaaagtgatt gcaaagaaat catcactaaa ttttggattt gcgtgatatc
     4441 atttgttaga cgaacaacaa gatttccagc agagaactgt tcgatatttc caaatgaaaa
     4501 tgtctggatc tttcgaaatg cttcttctcg gatatctgca cttacgcctt gagctacttt
     4561 tgcagagaaa atcgtgttcg ttacacctgc ggctaatcct agcaaagcca gactgatcag
     4621 gtaaatacca attgttttca tttcatcatt atcttctgta ataatagctt ccaatacttg
     4681 ctgtagtaac ttaggttgcc ataatgctgc tgcaaccatg actgctacgg acagcaagga
     4741 aataaaaaca gctactttat attttttcgc atgtcgtaag attaatttca ttcgttgatt
     4801 acaccccttt gtttaaattg acaggactaa tttaacactg taaaattaga ccgactaaca
     4861 atttaccatg tttaaaaaga taatgcaata cacgaggttg ataaaatgga aataaagctt
     4921 tctcaagcaa tcatcattca ggccgccttt gatatcctgg ctgaaacaca agctctttcg
     4981 cagctttcca tgcgaaacat cgcaaacaga attcatgttc aagcacctgc tttatattgg
     5041 tatttcaaaa acaaacaaca gctcttgcaa ataatggctg aaacaatgga agaagaatta
     5101 gcgcttccag atccaacttt gccttggaat gaacagttgc tgcagtatat ggaaaattac
     5161 tatgaccttt atacacgttt cccttgtggg gcagaattag aaatcaatac tgtgcccgct
     5221 tttccgagtc gattagaaca tttggagcaa atgatccaat tacttgttcg tgaaggattt
     5281 tcagtcaccc aaagtcatca ggccattgcc tctctccaca atttattgat tggacagtta
     5341 atggatcagc agcatgaaca gcagcttcga caaaaaatta tggacggaaa tgaatttttg
     5401 aaagaaacag ttctatttat gcgtaattat gtagttgaaa atcaattaga gggatttaaa
     5461 gaaagtatgc agtcccatgc agaattgaat gaaaaagcac tctttctaac gagtgcacaa
     5521 acctatattg aaggcttatg ccaacaaaat aaaagaagag aaaacgagca gtaaaaaagc
     5581 tcctatagag atcactatgt ttgtgatctc tataggagct atctttttaa gactcttttt
     5641 ttatttttct tccattagcc acgccaatgc acgttgaatc cattggtccc agaagatcca
     5701 gttatgatcg ccaggtcctt gttcaaaggt gaccgccaag ccttgttcct gcatagcaga
     5761 taccatttcc tgatttgcct gataaagaaa atcagattcc ccacaagcca tataaaatct
     5821 tggaactggt tttccgcttt ttactagttc tttcaataag tattgcggat cattttttga
     5881 accttcgatt tgatccaatg gtccaaagat cccttcccaa aaagatgctt tacgaaccat
     5941 caaaagatca tccattcgat ttcctacaga aacagcgcct gaaagagaga ttactgctgc
     6001 gaaattttct gattttgcaa ggcctagttt taacgcgcca tatccgccca tcgataatcc
     6061 agcggcatat gttttttctc tttttcttgt taattgcggg aataattcat gacaaatctc
     6121 aggtagctct tctgcaatga atgtccaata cttcatgtca taaactgtat ctgtgtacca
     6181 gccaagatca gtagatggca tgatgacagc taatccgtag tttgctacat aacgttcaat
     6241 cgacgtacgt ctttcccata cactatgatt gccacccatt ccatgaagta aatataaaac
     6301 aggtatatcc gttgtctctt gctctgaact ggtgccaatc accttttttg ttgtttgcgg
     6361 tagtatcaca tttagattga cttccatttc taacacattc gaataaatat ttgcttgaat
     6421 aaatgccata ttttcgcctt ccttagtaag aagttatttt tgaataactt taaatttgaa
     6481 tgattcggct acgtacatat cttaaccgaa actactgttt tattttacca cttttttcat
     6541 gaacgaaaag cgttaagacc aaagcaatga tcacgagtaa caagccaact agataaacgg
     6601 aatgtaaccc atcataaaga atttttctta atggtaacaa aatatccgca tcaatatatt
     6661 tagccgtttg tggattcacc agtttattca tgatatctgc atcatttgtt aaatttgctg
     6721 ctgcaagttc tcgtttagtt accccattta tcaataaacc aaaaatagaa accatgactg
     6781 tttgaccaat tgttcgaact aacgtattaa aagaagttgc tacaccaagc tggtcacgag
     6841 atacactatt ttgtgcactg acagtcgagg tagtcatggt cacacctaag cctataccga
     6901 tgaccgttga tatagcaaaa aaccataaat agttacttct gaaaggcata agatacaacc
     6961 aagcgccccc aatagctgcg attagtagtc caattctcaa gacccaatga gttttcattt
     7021 gagtcatcca cttgcttgca aggaaagaac cgaccatcca gactagtgat aatggtgcta
     7081 ataccagccc accaattgct gcattcagcc ctaatacacc ttgcatccac atagggatat
     7141 aaacatcgat tcccatcaaa aaaccactag ccaatgctgc agccaagttg accacaacaa
     7201 atagtggatt tttgaataaa tgcatcatga tgacaggatc tttagctctt ttttcggcaa
     7261 aaacaaaaat acctaacatt acgatactac ctagcagcaa agcagaaaca agcggactca
     7321 ttccttcgtc tcctaataat tggaacccta acagcaatga gacaagcatg gccatcaaac
     7381 taaaactacc aaaaatatcc attggtttag actcatgtat gatttttggt tcatttaaat
     7441 atagccaaat caaaataatt aatagaattc cgatcggcac attgataaaa aagatccagt
     7501 gccagctgat cgtttcaacg atgaatcctc ccgcaagcgg tccaaaaaca ctggcaatcc
     7561 cccaggctga actattcaaa cctaaaactt ttgctcgctt ttcaatagaa taaagatcgc
     7621 cgatgatcgt caaagctact ggcatcattg ccccagcacc catcccttga atcgcacgag
     7681 cagcaatcaa cccaatcatg ctttgagata aaccgcaaaa taacgaaccg ataataaaca
     7741 ataatgtccc aaaaataaag attggtttcc gtcccacttt atccgcaagt tttccgtaaa
     7801 tcggagtaag catagcgttt gtcagcaaat aaatcgaaaa tacccagttc atgatttcca
     7861 ttccatgtaa tgaaccgaca atcgttggca tagcggttgt tacgattgta ccttcaatag
     7921 ccgtcatgaa cgttgcaatg aaaatactca ctgtgaccaa agtaacattt gtttcttttt
     7981 tcatcttatt cctccctttc attatgacag gatctaaaaa tagataaata attactatct
     8041 ttttcaagag gttaactgcc ataaaaaaag ctggaacaac caccacttgg cgatcgttcc
     8101 agctgaatat aacttatttt gctaaacttg ctttcaacgc atctactttg tccgtatgtt
     8161 cccaaggcaa atctacatct gtacgaccaa aatgaccgta agcagctgtt tgtttgtaaa
     8221 tcggacgacg caaatcaagc atctcgatga ttcctgctgg ccgaagatcg aagttttctc
     8281 tcactgcagc aatcagttct tcttccggaa ccgttccagt accgaaagta ttgattgaga
     8341 tcgaaacagg ttgagcaaca ccaatcgcat aagctagctg tacttctgct ttgcgagcaa
     8401 gccctgctgc aacaatgttt ttagcaatat aacgtgcagc atagctggca gaacggtcaa
     8461 cttttgtcgc atctttacca gaaaaagcac cgccaccatg acgagcataa ccgccatacg
     8521 tatcaacaat gatttttctt cctgttaatc cggcatcccc ttgaggacca ccaatgacaa
     8581 atcggccagt cggattgata taatatttcg tttgatcatc taataattca gctggaataa
     8641 cttccttcac tactttttcg atgacatcat gacgaattgt ttcattatct actgcatcat
     8701 catgctgtgt tgaaataacg attgtatcta cgcgttccgg ttgtccttga tcatcatatt
     8761 caaccgttac ttgagatttt gcatctggac gtaaatacgt taattcattt gatttacgca
     8821 aatctgccaa gcgtcgtacc agacgatgac tcaaagcgat tggtaaaggc atcaattctg
     8881 gtgtttcatc tacagcaaac ccaaacatta aaccttggtc accagcacca atctcatcta
     8941 atacgtcttt tttatcttca tcccggatct caagtgcttc atcaactcct tgagcaatat
     9001 caggagattg ttcatcaatc gcaaccaata cagctgctgt atctccatca aatccgaatt
     9061 ttgcacgtgt ataaccaatt tcttttattg tttcccgtac gactttttga atatctacat
     9121 atgccgtcgt agatatttct ccaaatacta aaacaagacc agttgttaca gacgtttcac
     9181 acgctactcg tgctgttgga tcttgtttta aaattgcatc caagattgca tcactgattt
     9241 ggtcagcaat tttatctgga tgtccttcag aaacagattc tgatgtaaat aagtgtcttt
     9301 ctaccattat aatgattccc cctagttaaa ttcggttaca aggcatctcc gtcctgcgga
     9361 tcctttcggg aacttgcaac gaaccaagta tagcagataa aacagttttc tgctcgtatt
     9421 ttttcatttt tttgcttaaa aaatataaaa atattttaca ttagaactgt ccaaaaaagc
     9481 ttagcacaat catccataaa gataactctg ctaagctttt taaaatcatt ctttcagata
     9541 aaaagaaaaa gatactcaaa aagtatcttc
//
LOCUS       DASJPH010000106         9512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJPH010000106 DASJPH010000000
VERSION     DASJPH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.107"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..2206
                     /locus_tag="U3995_002431"
     CDS             149..2206
                     /locus_tag="U3995_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN1797722.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2244..4343)
                     /locus_tag="U3995_002432"
     CDS             complement(2244..4343)
                     /locus_tag="U3995_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1797723.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4644..5468)
                     /locus_tag="U3995_002433"
     CDS             complement(4644..5468)
                     /locus_tag="U3995_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797724.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(5465..6220)
                     /locus_tag="U3995_002434"
     CDS             complement(5465..6220)
                     /locus_tag="U3995_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1797725.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(6233..8209)
                     /locus_tag="U3995_002435"
     CDS             complement(6233..8209)
                     /locus_tag="U3995_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1797726.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(8262..8933)
                     /locus_tag="U3995_002436"
     CDS             complement(8262..8933)
                     /locus_tag="U3995_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1797727.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(8993..9202)
                     /locus_tag="U3995_002437"
     CDS             complement(8993..9202)
                     /locus_tag="U3995_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797728.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(9269..9508)
                     /locus_tag="U3995_002438"
     CDS             complement(9269..9508)
                     /locus_tag="U3995_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797729.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2812 a         1894 c         1496 g         3310 t
ORIGIN      
        1 aaatcgttga tattaatact tcaactaaca aataaaataa gaaaagccaa aattatataa
       61 ggcttttctt attttttatt gactatatcg gttagccgat atataataat catgtaaaca
      121 tttttaatta aatttaggag ttgaaaaaat gaaattattt aatccaaaaa ctgttttagc
      181 tattggaaca tttgctgcct cacttagtct tgctggatct gttgacgctg cttcaactaa
      241 cgtcatgtat cgcctttaca atccaaataa taatgaacat ttttacacct ctagcaaaaa
      301 agaacgtgac catctagtaa agataaaatg gggaaattat gaaggccccg cctgggaagc
      361 accgactagc ggtggccatc ttgtctatcg tttgtacaat aaaggtcttc gtgatcacca
      421 ttacacagct agttgggatg aagtgaaatg gttgactaaa aattacggct ggacttatga
      481 agggcctgcc tggcgttctg ccgaaaaaaa taataaaccg atctaccgcc ttttccttcc
      541 tggtgtaaaa tctggctcac atcactacac tgcaagttgg aaagaagtac aatggttaac
      601 caaagactat ggctggaaat acgaaggtat cggttggtat ggcgctgaca cctctaaacc
      661 caaagtgaat aaatcggctc tagaagctct ttatacgagc gttaagggaa cgaaaaaagg
      721 tgactatacc gacaatacct ggaacgcatt ccaaaccgct ttaaacaatg ctaagaaagt
      781 gttaaatgac tcaaaagcga ctcaaaagca agtggatagt gccaaaaata aattagattc
      841 tgcttacaaa ggattaaaga aaaaaccagt tcctactgtg aataaagctg aattacaagc
      901 tttatacaac cgagtaaaat caactgctaa aggagatttt acagaagaat cttggaataa
      961 ttttcagact gctttatcca atgctaaaaa agtactagac gattctaagg tttctcaaac
     1021 acaagtaaac aatgctaaaa atagtcttga tgctgcattt aaagggttgc aacacaaacc
     1081 tgaaacacca aaagaatata cggttactat taaccatatt gacaatcaaa caggtcaagt
     1141 gattgctaca gatcaagcaa aagtaacttc tggttctact tacactgcta aggcaaaagc
     1201 atttaaatat agcgaaggcg ctaaagataa ttttagctac aaagtgattg gtaatgatac
     1261 tcaaagcaaa acaatcacag gtaatacaac aatcacattc aattataatc aagtgcatca
     1321 aatcaatttg ttgtgcaata acaactacaa aaacgaagga cgagttaata ttttgaaaga
     1381 acagatagtc aatgtcactc acggtgacag tgtcacttta actgctcctt ctattcaagg
     1441 atatgtttta gacgatcggg tagaaccaac aaattcagta accttaaaca atgtgactga
     1501 aagtcaaaat tatattttca actacactcg taaatttaat gtgactgtta atcatgtaaa
     1561 tgctgatacc aacgctgttc tctctagtga atcaaaaaca gtatacgaag gggatagctt
     1621 tgctactcct tggaaaaaca tgactgatca aaattatttc ttgtgcagaa atgacgatcc
     1681 taccgttacc gtggataaaa acggccaacg atccattaat aacgtcgatc aaaataaaac
     1741 aatcacattc aaatataaaa agatttcatt agacgaatta cgtaacttta tgcgtcaaaa
     1801 agaacttgct tggttaaatg attatcgcca acaaaatggt gttgccccaa tgcaattcaa
     1861 tgatattgtt caacaggctg cagatattcg agctaaagaa ttacaagtca gcttcagtca
     1921 ctatcgccct ggtggtggta ctttccaaga tttattggaa agcttaggct gctacggtgc
     1981 taagggagaa aacataagtc aaactgggct gtatattgat gaccttttat cagacgatgg
     2041 tgctatcgat gccatggtag gatggaaaaa tagccctgcc cataatgcaa atttacttta
     2101 taataaccaa agtattctcg gattaggtca taattttgta gttgactctg ctggtagaat
     2161 tggcgtagat aatgtattta ttagtgcgaa tccaatcttc aaataaaaga aaacaaggct
     2221 gattctcagc cttgttttta tatttatttg agttttaaaa ctacatagcc caatgatgct
     2281 aaagtaagaa ataatatact tccaataaca gttagcacta aatgattcga tccagttttt
     2341 ggcacaatga tctgaggtaa gttaggtttt accccaatcg ttactacatt agttgctttc
     2401 tcaatacttt ctactgaaac ttttgcttgg ttgtcgatat ccaattcttc attcaaagga
     2461 gttttagctg taaatatcag ctgaatttct cgtccatcta aaagattttt acttaagaaa
     2521 tcagcggaag cttcccactt cagcgttctt gaattgctat cataggttag tgtaccttct
     2581 gaagtaatat cttttcctgt actcatatct atcactcgcg cagaaccttc tttatacgtc
     2641 aaacgatctt ccactggatc ttgaatacta aattgcttgt aatgatctga ggcaatgtct
     2701 acaccttttg tatgccattt ttgtgtgact agatattgta cttcagatcc tgcatttaaa
     2761 ggagctgtag gcgttccttt tttaccatca tttcgaccat caacatcttt cccatcttgt
     2821 tccacttttt tatttggttt aagagagaaa tagttttcta ctgtatttgt tttttgtgtt
     2881 cctttgttcg gatcattgtt aaacgttaca gtagccacat ttggtgcaat tccttttgca
     2941 tcattttttg ctctcatatg gagctgcaaa atataatccc taccgtaatt tgcagcgtta
     3001 gctaaaaagc tgtcagaagc agtataagtg actgtattac ctgatacagt taactttcct
     3061 cctgtatcag cttttttcca agtaccattc gcttcaacat accacaatgc gccatcatag
     3121 tttttcccat ccgataccat atcaaaattt ttgctgtcaa aggtatcagt gattttaaat
     3181 gatttgtatt tgttcattgt gttgactgtt aatgcatcaa acttttggtg aatatgatag
     3241 taaacatctg atccttgtgg aatcactttt ttgtcaatat tgtgtgctgc ctcttcagta
     3301 ttagctttgt taaacacttc ttttgtaggc ttcgatggtt gtggtgtcca tagcaatgca
     3361 gtatcaaagc taaacatcat actattcgta tttgcgccaa tcatgaatgt tggggaagcg
     3421 ccttgaagcg tatacgttac accacttctg tgataagtat cagctcctac aacatcggtg
     3481 aaatgatcat tcgagctata agccttttct gcacgtcctc ccactacttt tgaagttaaa
     3541 ttgtttgttg ataacgcagg agctttgtat tctgcacatg ctgtatctgt tgtgacaaaa
     3601 taaggtaagc tacccatttt ttcataccca acaaattctc tcatatcaat attacgttca
     3661 ccgacagatt cattattgtc attataccaa gacacacgtt gctgatactg tccatttaaa
     3721 ctgtaaatgg atagaaatgt atcgtttaaa tcaataccag ctgctctcat cgatctcatt
     3781 tttgattgaa tttctggctg tggttcactc aaagctactt ttgtttttgt tcctgcatac
     3841 ttaaattcta gttttgcatt catgacattg attgactgca tgaaaaatcc tcgataaata
     3901 tttttatcaa agagcaaata attataatac tttttgccat taattgtttt agctaatccg
     3961 tcttggttaa ccccagcgtg cgctacaaaa ttagaaaaag tcacgtcaca atcaatttct
     4021 tttcctagat aagtacctac tttttgataa tgggcaacga tttttgacgt acttttcaaa
     4081 tctttgactt ttggccaagc aagtactttt tgttttttag ttgaatactc atctagtatc
     4141 ttaaatccat tgatcccagt tacttcgata tttctcaatg aatcactatc atgataaagc
     4201 accagattat tattttcttt gatttgactc ggatcaactt tttctaaggc ctggactgca
     4261 gttcccctcg caaaaagaag aaggaacaaa aacaacacta aagtaattac tcttcccgta
     4321 gtgcaatgaa ttttattttc cataatgccc tccatctaat atttttttaa acgatcgtaa
     4381 gcttttcatt ctttttttcc taatacccac gttaccaatc ctgtaagaag aaccaaacta
     4441 accataaaaa ttggattgat caagagcttt ttaatcacct ctaatggcat ggaagcacat
     4501 tgaatgttta gaaaatgaat gaacaatcgt tgatatcttg gataaattga taattgagaa
     4561 aaaattaaac cgcaacacaa tattgttgcg gctaaaaagg atagattaaa aactaatatt
     4621 cttattattt tcatcatttc atcctaatta tcaaaaaata attgagcaaa aaattcccaa
     4681 caattttcaa aaaagttctt ctttacacca gttgacacaa cttttttaat ttcttctttc
     4741 ggctctgatt tctctgattt agggtataac caaacatccg tcaaaaattc acctttttca
     4801 tttgaaacag gtgtcaataa aacaatatct tcactgtttg aaatgttttc agggcttgat
     4861 tcattaacga tatagtatgc ttgttttttg tttgcaggta cttcaatgga aaattcaatc
     4921 acgccttcct gatcatcaat cgttttggtt tctccttcag ccactttttt caactggtcg
     4981 tatgaaagtt tttttgctcg atctttcaat tttgactcaa ttatttctac agattgattt
     5041 ttctcattat catcttgtga catttcttct aagatattcg tgatatcgta gacaataaac
     5101 ttcgcgccat tgataccttc ttgctgggct acttgactgc cttgattgtt gatttgattc
     5161 gaagaaataa ttttttgaat atgaatggct tgcttgatca cttgcgtatc ttctacgtct
     5221 tgcggctttt ctgaagtctc tgaagaactt tctgacgttg gattcgttga ttcagtaaag
     5281 cttatatttg aatctgccgt ttcttctgta gtattctccg aactgtccgt ttcgcttggt
     5341 gcagtaattg aattatctat tgtttgagtg gtactactat cactgctgtc agcgaaagac
     5401 agttgcgaat aagctacagt agaaaagaga gctgctacac aaacaaacag ccattttctc
     5461 ttattcattc cgatctcctc cttatttttt tataaataaa aataatagga acgcaaaaaa
     5521 aagcgaatag cagtgctaat cgccaatcca atgaaattga tttcctattt tttgcttttt
     5581 tctgtagttt taaatcttct ttactgaagg aaactcgttc accatacact aaaaagcggt
     5641 gtgagttgac accttttggt gtgcatgtca ttaacgtgat cctatcttgg tctttttcag
     5701 gcataagata atctaaataa tttttttgta attcttctgc tttaacttct tcaattcgat
     5761 aaatttgata ggcatgttgt tcttcattta ccttaataaa gaatttttgc ccttttttta
     5821 ctgaaggcaa atcatcgaaa agattatctt tataaagtcc actgtgcccc gcgattaaag
     5881 gatttttacc gtttcctcct gtaatatcgc cagttccttc cgtaatccct acaccattct
     5941 ctaaaatttt gtcggaagtt ccgtcataaa ttggtagttt taaagaaata cttggaattg
     6001 taagaacacc aacaggatcg cctaactcta ttttcgcaac atccaaagaa gaatctgttt
     6061 tttcaagatt ttcaatggct aaaattcttt gctgttgttg aaatttcgct acatttgttt
     6121 ccacaactgg aattaaatag actgctactg ccgacagcaa ccaaaaaaat atcatcagtt
     6181 tgtgcatgac tcttcttttt gggggcttac gaataatcat tagctgatcc ttctaggctt
     6241 tcttcttaaa gtaaacaact gcagaaagtc cccctgctcc tagcaacaag caagagatca
     6301 atacgtaaga ttggaaaccg ttgctaccag tcattggtac ggaagctttt tgattatttt
     6361 tgatcgtgat tcggttttct tctttgtatg aatctttgtc tactttgaaa gagatctctt
     6421 ttgttaattt ttgatagttt tcaggagctt ttgtttcttc cagtgagtaa tctccttcag
     6481 ttaaaccagt gatttcaaaa tgaccctctt tatctgattt taggattgct gcatttgcta
     6541 catcgtctac ccaaactgct tctttgacac catctttatc tgtatcagta agtccaccgt
     6601 agtacttacc ttctgcattc ttagctgcaa attctgcgcc ttcaagcggt gttgctttga
     6661 tacctgttcc aaataggccg ctatcgactt ttaagaattt tgcgccataa gtcacaacgt
     6721 cagctttcgt tttttcgtgg tgttcttcct gattaaatgg attattgtta taaacgaaat
     6781 ccacgctatt tgttaatgga tttgcgattt cagcactatc attcacaaac atgttataag
     6841 tcatttttag atcttgacca tgtaaattcg caactgtatc acttacttgt ccattattta
     6901 cgataaaatc gatcacaaaa ccactatcag aattttcgat tttatagtca gtattttctt
     6961 ttaatacgac tgaaccatca ttatttgtta ctttaatatc tgctttttca gcaacataag
     7021 aaagtccttt acctgctgta tctccccatt taaatacact gtaaggagaa gtttcgcttt
     7081 tcacaccgtt tactgtgata ccgtctgcgc cacctgcaat gtctgttgga atatgaatcg
     7141 ttccttgata atttactgcg tcaccaattt ggaaagaatg ttcttgtcct gttccatttg
     7201 ttactgtttt atcgatcact ggtgctttat agtttacgta tgaagctttt aagtcacttg
     7261 tcacgccatc attcgtaatt tcaaaggtta atttattgtg cgcatcgttg cgagcgtctg
     7321 cgcctaataa atattgatct gctgttggtt cttgatctga tcccactaca acttccgaag
     7381 gaacttccac aaaatagtat tttcctaacg tcaaatcagt cgcagtcagc tttccttgat
     7441 catccgatac aagatcaccc aattttgttc cttttccagg ttcgcccttg tataactcaa
     7501 attttgcccc ttttaaaagg gtttgtttgc tttggtctgc gccatcgtca ccaaatttcg
     7561 ttaattcaaa actcaactta gaaacaatgt tcttaggata caaatgaata ttttttaaaa
     7621 atcctgatcc tgttttatct gtcattggcg caatcacaac cattggtttt gctttttgtg
     7681 tgactaatcc agcagcggat ttactttcat aaaccacata tacattccct ttggaattga
     7741 cataggctgg ttgatcagta aaagtgatct caccattttc atctacagct gaagtaattt
     7801 tgttggttgc attcttgacg tactcactgt tagatccctt gtcttctaca tcttgtacaa
     7861 tctcgtccac tttcgcgttg gttagatcag aatcttctct cggtaatact tgatccgtaa
     7921 tatttcctaa cgtaaactca acatcaccat aatctgcctt gttgtatttc gttacacctg
     7981 taggatattg atcgtgcgta taaccatcgt ttttgatccc gtcaatatca acattcaatt
     8041 gcgttccttg atcgtacatt aatttatgaa ccgtgatatt gaccttctct ggtggtgttt
     8101 tttgcgtgac atcttctgca tgtacttgat acccctgaaa gcagcctgca atgattccta
     8161 acattccaac aagcattcca gcttttttta cataccgatt tattgccatt ttttactctc
     8221 ctcatttcat atttcaacga ctcgcgttga ttaaaacctt gttattgtag ctctccctgt
     8281 accacgtatc tttcttttcc ttgtgacgta taaacacaag taaacaacgt taataagggt
     8341 ttctctcctt tctttggcaa agacaagaaa gagttctctt caggaacgcc atcgattaat
     8401 ttaaagtacc caggtacgat aaatttacgc gttaccttgt aacgatagac gccatcgtta
     8461 ctaactaagt ccatcacctc ccctaatgac aacttttgaa tatcagaaaa taaaacacct
     8521 ggcatttcca tgttatgacc tgctaggaca tagtttcctt tgcccatttc ggcatcttca
     8581 taaaaataag tagccgctcc aagactcaaa gtgtattgat ttgccccttt aaaaattggc
     8641 aatgctaaac caagtgcagg aatctgaatt tgtgcaaata cttgtttatt gacgcccatt
     8701 tcgattgctt ctttttgtat ggacaacatt tgccccaaag acatatcatt attgacgttc
     8761 gttgcatcct ccttataact ctttgcatgt gcattgattt gatctacggg catctttttt
     8821 tctatatttt gcttaaacac ctctccttca tgtttttggt agctaacata gccaaacaca
     8881 ctgcaaaaga cgattaaagt gactcctaaa aaaccaaata accatttttt catccatctg
     8941 cctcctttca gacaaaacaa aaaaactttt tctcatttcc atagaaaaag ttttaaaaat
     9001 gaattctata agttcgtctc aactgttcaa agtttttcat tgaacctaac aaacaagctt
     9061 tctcttttcc tgttaactgc atatcattga tatattgcgc gacagataat cttttaaggt
     9121 gtttttgctt catcgttttt agtaactttt cttccatttc tttcgacact ttttttctgt
     9181 tttcgatttc ttcataattc atgagttttc ctaccttcct tgttttttgc aaaaataaaa
     9241 aagcctatga tcacatacgc tctacacttc agtcttgggt tatctttttc tttcgtttaa
     9301 ttactggccg tgctggtttc aaatccttga ttccattatg ggttctccaa taatcgaatt
     9361 cttttcgcaa tctttctttt tccatatctg ttcctttaga taaggctttg aatacttctt
     9421 taggtgaata accacgagct aacagctgta cataccttcc ttttgtcaaa gtatccatat
     9481 ccatcgcctc taaaactggt cgcgccatct ac
//
LOCUS       DASJPH010000107         9195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJPH010000107 DASJPH010000000
VERSION     DASJPH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.108"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..886
                     /locus_tag="U3995_002439"
     CDS             11..886
                     /locus_tag="U3995_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797730.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1031..1183)
                     /locus_tag="U3995_002440"
                     /pseudo
     CDS             complement(1031..1183)
                     /locus_tag="U3995_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1451..2782)
                     /locus_tag="U3995_002441"
     CDS             complement(1451..2782)
                     /locus_tag="U3995_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797731.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2931..3644)
                     /locus_tag="U3995_002442"
     CDS             complement(2931..3644)
                     /locus_tag="U3995_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797732.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3819..5243
                     /locus_tag="U3995_002443"
     CDS             3819..5243
                     /locus_tag="U3995_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1797733.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5542..6249
                     /locus_tag="U3995_002444"
     CDS             5542..6249
                     /locus_tag="U3995_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1797734.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6236..6559
                     /locus_tag="U3995_002445"
     CDS             6236..6559
                     /locus_tag="U3995_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1797735.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6648..7685)
                     /locus_tag="U3995_002446"
     CDS             complement(6648..7685)
                     /locus_tag="U3995_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1797736.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8006..8989)
                     /locus_tag="U3995_002447"
     CDS             complement(8006..8989)
                     /locus_tag="U3995_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1797737.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         2862 a         1777 c         1581 g         2975 t
ORIGIN      
        1 ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg aacgcggtta
       61 cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt acttgtccgc
      121 aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa agaccaacat
      181 gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt
      241 attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg aactctcgtc
      301 ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga ttcttataga
      361 acatccgcaa tttatcgaac gcttcaaagt taagatgcca tttcatgcaa taaattattc
      421 agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa acaaaaacag
      481 gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag ttgaatcgct
      541 cattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga ttgctactct
      601 cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag aggactgtta
      661 ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc aaatgttctc
      721 ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct actccataaa
      781 aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa aaatagataa
      841 aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag taaacgataa
      901 caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac gaatcatgca
      961 aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac tattaggtac
     1021 gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tgggtagatt ttccggcatt
     1081 gttgatagat tttatgatac ttttctacat tttgtaagag aggttccact gcatctgtat
     1141 attggataaa attctgacaa caactttcag gtgtgtcgaa ccactctaat ctttaaagat
     1201 aaatattaaa ataacgatta ataatattaa cagtctttat ttttcaccac caaaaaccta
     1261 tctttgcata tactaaatag acgtatatac acagataggt ttgtcttgtg aaaaataatg
     1321 attatcctta ccaaaaaatg tttgtttttc tacatgttcg tacttatcaa aaaatatgat
     1381 ccttattgaa attactggct atttatattc caacgacttc ttgtgttaat ttttgttatc
     1441 tcatagtaaa ttatgtttca ttttcactta atttagttgt cacgtgttcg tcaattcgta
     1501 aaaacggtaa atatataaac acacttgcgg ctataattaa tatttgtaaa acagctgctc
     1561 gccagtctcc ggctgttgct aaaaatcccg atataactgg tggcgtagtc caaggaattt
     1621 gcactactac atgattaatt aatcccacag cagttgcaaa atatgctact gtcaaagaaa
     1681 aaatcggtgc aagcacaaag ggaataatca gactgatatt aaatacaata ggcaatccaa
     1741 aaataatagg ttcgcttata ttgaatatag cgggagttac cgataattta gcaaagtcct
     1801 tataatcttt tctacgactg aagataaata ttgcaatcaa taaagcaatc gtatttcccg
     1861 atccacccat taccgcaaag gtatctttaa aggccatatt gataatatgt ggaatttgct
     1921 ctttgtttgc ataagctgcc atattttctt gcatattctg aatcaaaaac ggatctaaca
     1981 atgaacctga tattactgct tgatggatac caaatccaaa aaatagatta gcaatagatg
     2041 taattaacaa aaatcctggt aaaccagttg taattttact taatggctct gtaactatcg
     2101 tggtaattag cttatttata tccatattaa acaattgaat gattataaat gacaataaag
     2161 caaacgaggt aatcgtaata atgattggga ttaatacatt aaaagacttt aaaacagcgg
     2221 gaggaatatt tccggatagc tgaatctgta gtttcttatt attagaaagt ttaataaata
     2281 attctgtagc cgctaatcca acaaatatac ctacaaacat tcctgaagct cctgtatagg
     2341 atataggtat cacacccgtc acttcaacag ctttatccgc attttcagga gtaaacatca
     2401 tctgcatagg tgttaataca acaaatgtcg aaatagcaac taataccgct gcaatatgat
     2461 ttgtataatt tctgcttgca cacatatgat aagatactgc tgctacaact aataatgtag
     2521 ttatgcttaa tgtaccattg gcaattgaag taccaatttg ttgtaacgtg gtcaatgtac
     2581 tagtatcgac aatttccccc atgaatcctg ttggttctaa gatcactgaa ttaataagtg
     2641 tcacaaatcc tgctaacatc ataaatggca taaatgtagc aaacgcatct cgcaaagttc
     2701 ttaaatggat ctgattcccc aacttagtcg caactatacc aaatttttca aatacaatgt
     2761 ccttcctgct acttttgccc atattttttc tcctttatgc ttattgtaat cgattacttt
     2821 ttccacccaa ataatacatc acttatcaaa aaaagtaaat acttttaatt aaatatatat
     2881 atctttttta gtataaaaaa ataagctctg aaaaaataat tttcccgagc ttatttttca
     2941 acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga atactcgaaa
     3001 ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc acctggatct
     3061 atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg tatgtgcgaa
     3121 gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt ctttaaatgt
     3181 tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat cgagtatggt
     3241 tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac actgatactt
     3301 aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat ttctgaatct
     3361 acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac tcgtgagtta
     3421 tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa aactccttcg
     3481 gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata aataactgcc
     3541 aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac aatttttttc
     3601 tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga ccctccaatc
     3661 aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca catcaaaatt
     3721 atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta agttgtaagc
     3781 ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg aaaaagctaa
     3841 cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca gaaggaaaag
     3901 gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa ccttaccggt
     3961 tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat aaagaagata
     4021 tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct tgggcacgaa
     4081 tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc tatcgaaaat
     4141 tattttccga aatgaataat aacggaatca aagtatttgc caacttatat cattttgaca
     4201 tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt gatgcttatg
     4261 ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat tggtttacct
     4321 tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat tatccaaacc
     4381 tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca cacgcaaaag
     4441 caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt ctaaatttga
     4501 gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa atcgctgatt
     4561 tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct aaaaagctta
     4621 tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa gatcttgtat
     4681 gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca cgtcgcgtca
     4741 aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg tttttcgaac
     4801 cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt tacgaaaaag
     4861 gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa gcatttatct
     4921 cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca gggcaaataa
     4981 ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg aaggcaataa
     5041 atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc tggtcatgga
     5101 tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact caaaaaagaa
     5161 caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga tttagatacg
     5221 atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac acattagaaa
     5281 acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca tttatacttg
     5341 tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg gaaatctaac
     5401 ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat tgaagttgac
     5461 aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga aaatttatgt
     5521 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
     5581 acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat cgttgggaaa
     5641 gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta tgctggttct
     5701 gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc catcgttctc
     5761 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
     5821 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
     5881 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
     5941 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
     6001 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
     6061 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
     6121 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagtaac
     6181 ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
     6241 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
     6301 gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc ttgagtattt
     6361 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
     6421 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
     6481 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc tttccctttc
     6541 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
     6601 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
     6661 ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt ggttgcttgt
     6721 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
     6781 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
     6841 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
     6901 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
     6961 acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
     7021 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
     7081 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
     7141 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc agctccgttg
     7201 atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc aaatttttca
     7261 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc ttcatctgaa
     7321 agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc ttgattgctt
     7381 cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
     7441 tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg taaagcattt
     7501 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
     7561 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
     7621 tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa ttccttttct
     7681 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt ttcatttcaa
     7741 tatatatagt atgctatttg ttccactttt attcatcata attattctct tacttaaagt
     7801 aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg tcacaacctc
     7861 ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacga caaaatatga
     7921 ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacgacctc
     7981 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
     8041 caaaggtgtc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
     8101 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
     8161 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
     8221 gtccgatcag ttctttttct gtatgttccc tcggttcttt ttcaaatgca taaggattct
     8281 tgaaaatccc ccggccaatc atgatgccat ctacgccata tttttcggcc aattcttctc
     8341 ctgtctggcg atctgggata tctccgttga tcgtgatcag tgtttgcgga gcgatttggt
     8401 cacgtaaagc aatgatttta gggatgactt cccaatgtgc atctacttta ctcatctctt
     8461 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
     8521 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
     8581 cgccagcttt agccgcttca atcaattgtg ccgctacttc tgggcgaagg atcaagccac
     8641 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
     8701 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
     8761 aaatatgggc aaccatcggc tgttcatctt ctgtaaaggt caatcttcct cgtacactgt
     8821 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     8881 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     8941 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     9001 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     9061 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaag
     9121 actgtgacaa aaatcttatc acaattcctt ttccggaata aatggtgttc aaaagcttgc
     9181 atctgcggta ataag
//
LOCUS       DASJPH010000108         9149 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJPH010000108 DASJPH010000000
VERSION     DASJPH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.109"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..282
                     /locus_tag="U3995_002448"
     CDS             <1..282
                     /locus_tag="U3995_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1797738.1"
                     /translation="APVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGR
                     YIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(606..689)
                     /locus_tag="U3995_002449"
     tRNA            complement(606..689)
                     /locus_tag="U3995_002449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Leu,seq:cag)
     gene            1217..1717
                     /locus_tag="U3995_002450"
     CDS             1217..1717
                     /locus_tag="U3995_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797739.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1907..2251)
                     /locus_tag="U3995_002451"
     CDS             complement(1907..2251)
                     /locus_tag="U3995_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1797740.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2405..3325)
                     /locus_tag="U3995_002452"
     CDS             complement(2405..3325)
                     /locus_tag="U3995_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1797741.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3512..4003
                     /locus_tag="U3995_002453"
     CDS             3512..4003
                     /locus_tag="U3995_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1797742.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4027..6402)
                     /locus_tag="U3995_002454"
     CDS             complement(4027..6402)
                     /locus_tag="U3995_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1797743.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6728..7354
                     /locus_tag="U3995_002455"
     CDS             6728..7354
                     /locus_tag="U3995_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1797744.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7377..8081
                     /locus_tag="U3995_002456"
     CDS             7377..8081
                     /locus_tag="U3995_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1797745.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8273..9106
                     /locus_tag="U3995_002457"
     CDS             8273..9106
                     /locus_tag="U3995_002457"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1797746.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2776 a         1749 c         1627 g         2997 t
ORIGIN      
        1 gcgcctgttg ctgaaacaca acctgctcct gctgtaacag aaactgctgc tactccagca
       61 agtacaagct ctgcaaaaga atggatcgca caaaaagaat caagcggttc ttacacagca
      121 acgaacggac gttacatcgg ccgttaccaa ttagattctt cttacttaaa tggtgactac
      181 tctgctgcaa accaagaaag agtagcagaa caatatgtta cttcacgtta tggttcttgg
      241 gaagcggcga aagcattctg ggaagcaaac ggttggtact aagattcatt atttgaacgt
      301 ttaaacacat tcaaaatata gtaagaggtc atgcgattta ttctttcgca tgacctcttt
      361 ttgagtttaa gtttagtctt tattccttaa ctatcgtttg ccagtatttc ttgtatcaga
      421 atctataaat ctactatttg aatcaatcta ttaggtttta aactgtccca ccttcacaca
      481 aatttttttt gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt
      541 agataatatg ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca
      601 aattatgcgg ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct
      661 agcgcgtctg ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt
      721 tttttgtcaa gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc
      781 tacacaaata attcatccga gcaactccat catctattga gggttggtga aaaaatctgt
      841 tttatccata tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta
      901 tttttttctt catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga
      961 tattgattat ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc
     1021 acgttttctt tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt
     1081 attgtaattc atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa
     1141 aataggcatg gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact
     1201 taaagaggtg aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta
     1261 tcagctacaa actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta
     1321 tacatttgct gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga
     1381 tcttcctgag agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta
     1441 tgtaggaatt tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca
     1501 tattggttac ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc
     1561 attagtacta caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa
     1621 aaaaaatctt ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga
     1681 tgagaaagaa tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa
     1741 gggatttgaa tgtcctttat caatagaaca catttattta attaacttat aatatagcag
     1801 acaaaatttt ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct
     1861 tttttgtttt tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca
     1921 acgcaatccg tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt
     1981 tgacttctgc tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta
     2041 aaatatctgc taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat
     2101 tttcaggatt gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt
     2161 caggatcaat caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt
     2221 gtccagaccg ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat
     2281 ttatcatact ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta
     2341 aatttataaa aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga
     2401 ttcctcattt tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca
     2461 ttttttgttc gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga
     2521 taaatgcatc gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg
     2581 tgatgaccat catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta
     2641 atgtcattcc acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg
     2701 taacgatcat tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca
     2761 ttgcataaat ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga
     2821 tgactacaat gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt
     2881 gtgaaagtac aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata
     2941 atcctacgcc aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag
     3001 tgatctgtcc gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac
     3061 taaggatcgt tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca
     3121 aaagaaaaat aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg
     3181 aaatgatttg agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat
     3241 aatttaaagc gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc
     3301 tttcattttt atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt
     3361 attccaaatg taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag
     3421 aagaatttta gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt
     3481 aaatatttat atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt
     3541 atcagaagtc aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc
     3601 caatcgcttt gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt
     3661 tgtattgagc gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct
     3721 gatcaaaaga aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg
     3781 tggcaaagct cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa
     3841 aatcactaca gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca
     3901 gattagagtg acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa
     3961 acttgactta ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga
     4021 cgtctgtcat tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat
     4081 tccttcttcc aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa
     4141 taaacgtcga taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc
     4201 cgccatgcca cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg
     4261 ataagcgtaa gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg
     4321 ctcatgattc atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc
     4381 tctcaaatcg attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc
     4441 agcgggattt gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt
     4501 ttcttggatt agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc
     4561 taaatattga taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc
     4621 atgaatcgtc tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag
     4681 gtaatttgcg atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc
     4741 cagacttttt tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga
     4801 aaaaagaata ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat
     4861 acaaaactct tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc
     4921 atcataaaat tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg
     4981 ttcttgtgat gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg
     5041 ataacctgcc tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc
     5101 ttcttttgct atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg
     5161 aagttttttg aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag
     5221 ttctgccgaa tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa
     5281 cagatagtcc tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa
     5341 taaataattg taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc
     5401 gaaaggacaa agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt
     5461 ctcatgattc aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata
     5521 tgggcactga tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt
     5581 caaagtgaca ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc
     5641 tacttgtcta gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc
     5701 tacagctttc aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc
     5761 tacaaacaat ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg
     5821 acctttccta tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt
     5881 gcgccagttt tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa
     5941 agtttctaac tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt
     6001 ctggaagtaa gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata
     6061 ggcatacacc cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa
     6121 tcgtggttcg gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc
     6181 gtctgcacgg ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa
     6241 gacttctttt tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc
     6301 ttctaatgca gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa
     6361 ttctactaag tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc
     6421 tctaagatta cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa
     6481 aacaccctgc tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta
     6541 atgtaacaag gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc
     6601 aaatactttt catatagtct gataattcag ataagtaaag agaaataagt acctgttttt
     6661 gtttcctagc aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg
     6721 aatgcacatg catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt
     6781 ctacaacgaa gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca
     6841 agaaatcaaa aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga
     6901 tcatatcggt gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc
     6961 actagagcaa aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga
     7021 tgatattgct gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac
     7081 tttaggaaac atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag
     7141 ctttattttt gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag
     7201 aaccgatctg tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt
     7261 tgtattacct gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca
     7321 ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga
     7381 cagaaactac actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag
     7441 tacaaggctg gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag
     7501 gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa
     7561 tcgaaactgc ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta
     7621 ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa
     7681 tgtggggtgt gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag
     7741 aagtttcttt tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg
     7801 aaacttatga aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact
     7861 gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt
     7921 tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa
     7981 caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata
     8041 tcgataacgg gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt
     8101 accagagaat cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt
     8161 gtacttatct gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc
     8221 aaaaaataac ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt
     8281 tgcaaatatt atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc
     8341 catcagtagt actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc
     8401 acaatttatg tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt
     8461 tctttatttt cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga
     8521 tacgtggaca ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt
     8581 gcctttggat gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt
     8641 gatcgtttac ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg
     8701 ttggacttct ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt
     8761 cttggcactt gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat
     8821 tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt
     8881 tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga
     8941 agaaactgtt gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat
     9001 caaattctta ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat
     9061 tatccttggt atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca
     9121 cttctcttat ttgagaaatg ttctggttt
//
LOCUS       DASJPH010000109         9092 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJPH010000109 DASJPH010000000
VERSION     DASJPH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.110"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..340
                     /locus_tag="U3995_002458"
     CDS             <1..340
                     /locus_tag="U3995_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1797747.1"
                     /translation="DRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLP
                     ETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIM
                     IRHGKTFLTK"
     gene            460..660
                     /locus_tag="U3995_002459"
     CDS             460..660
                     /locus_tag="U3995_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1797748.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            736..933
                     /locus_tag="U3995_002460"
     CDS             736..933
                     /locus_tag="U3995_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797749.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1230..2777)
                     /locus_tag="U3995_002461"
     CDS             complement(1230..2777)
                     /locus_tag="U3995_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN1797750.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(2861..3664)
                     /locus_tag="U3995_002462"
     CDS             complement(2861..3664)
                     /locus_tag="U3995_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1797751.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(3667..4449)
                     /gene="phnE"
                     /locus_tag="U3995_002463"
     CDS             complement(3667..4449)
                     /gene="phnE"
                     /locus_tag="U3995_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1797752.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4446..5225)
                     /gene="phnE"
                     /locus_tag="U3995_002464"
     CDS             complement(4446..5225)
                     /gene="phnE"
                     /locus_tag="U3995_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1797753.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5197..5988)
                     /gene="phnC"
                     /locus_tag="U3995_002465"
     CDS             complement(5197..5988)
                     /gene="phnC"
                     /locus_tag="U3995_002465"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN1797754.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6049..6978)
                     /locus_tag="U3995_002466"
     CDS             complement(6049..6978)
                     /locus_tag="U3995_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1797755.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7173..7856
                     /locus_tag="U3995_002467"
     CDS             7173..7856
                     /locus_tag="U3995_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN1797756.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8017..9060
                     /locus_tag="U3995_002468"
     CDS             8017..9060
                     /locus_tag="U3995_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1797757.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2788 a         1869 c         1796 g         2639 t
ORIGIN      
        1 cgatcgactg ttatctgctt gtcccaacct gcgccaaggc tatcagttat accaagacat
       61 tctttatgcg gtaaagaaaa gagatcagtc attatttgag gactgtttaa acagagaagt
      121 cagcggtctt cctgaaacct atacaaccac tttacgaacg tttaaaaagt ttctgccaca
      181 gatccaaaat gcgcttcact acagctattc aaatggacca ttggaatgtt taaacaacca
      241 catcaaagta ttaaaacgca atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg
      301 gatcatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga aagagagttt
      361 cttctttctg ttctgtccaa attattctaa gatttctctt taccaacatc atttgacaaa
      421 gagcctgtat ttattactgt cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa
      481 aacatttggg atcgctatta taaagccgat ccgtcaagta aaaatacaaa gataggcgag
      541 tcaggtcttg gattatcaat cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa
      601 gtgaccagcc gaaaaaatga aggaacgacc ttcctgatac aatttccaaa agaaaaataa
      661 aattaccagt aaaaaaccgt ggagagatag ctgctattga tttttgctat gtcttttctt
      721 tgagaggtat ccggtatggt ggagtgtctg aaaaataaaa aaatggaggc caattttgtg
      781 aaagctgtta tttctggaag tattgttttc caagaagaat atcgagatat ggaaaaagat
      841 ttcagtaaaa aaggcattga attaattgat tatcctcgct catgtccgat cctagacaaa
      901 gagtatccag aaatcttgac gacttttttt taaatatcgc agcagcggat ttattttttg
      961 tatttaatca agatcggaaa ggggtattag gctatattgg tgccgcaact ttttcagaaa
     1021 tgaagtattg tctgatgcaa aatcttattc atggaaaaag gatcaaaata gtcttattta
     1081 aggaaccatc agaggaaaat tttttgtttt gatgaggtag atcgctggct aaagaatggc
     1141 tggatggaaa tcagacagcc taacaatttg gcataacaaa ggtgtgggca aaaaactgct
     1201 cacacctttt tgaatttttt atttatatac tacaaaatca cttgaaaatt atgattcact
     1261 tttgcttcga tgcgtgggtg aatattcaaa tagtcggcaa ttaattcggt catgtcgatt
     1321 tggatttctc ggacaatttt ctccgcacca aacatcgcat aatttcctcc gccgattgcc
     1381 cgatattgat ttgtcaccac ttccagcatt tgttgcggaa gaacggggtg acctttaaaa
     1441 cgaagttcgg taatccgttg tccaacaggc tgtttcatat caatagtgta ctgaatccct
     1501 tcatacatgt cataattgta gtactggggc ttaggctgaa taaaggctgg attgaatacg
     1561 atttggttat tttctacagc taaataattt gcggtctgct ccaacgccgc tctcaaatct
     1621 gcaccattta tttgaatcac tgccagagta ttcggataaa tatagttggt gatgacgtcc
     1681 cgcatcgtga tcgtctcacc aaagcctttt ccttcattat tgaataacgc agttccagaa
     1741 atctctgagc cgctggcaaa taattgtaca cgattaataa actctacata gggatgttcc
     1801 tcaagacgag cctgcatcgg atcattaata cgcatatttc cgttcacttt acctaagggt
     1861 tgatccaacc atttctctag tttttcttct accggtacga tcgctgcaag aatttctgga
     1921 tcaggctctg atttttcaac tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc
     1981 ccctcttttt tctcgacggt aagacgaatt tcaccgatga actcgccacg atagcctggt
     2041 tgaacaacgg gaactccatg aaccgtttca gcaattttcc ggtgctgatg accggtgaaa
     2101 agggcgtcaa tgccttcaac ctgctgcaat agctcgtagc cttcattctc tccagttagc
     2161 gcttctgtgt cctcaccggt taacaagtct ttttcaaatc cgccgtggta actaacgatt
     2221 accaagtctg cttgtttcct taatagagga acatattctt ttgctgtttc gacgatactc
     2281 ttaaacgtaa ttcctgctaa ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt
     2341 aatcccaaga tagcaatttt tacacccacc ttttcgataa tcttataggc gtttccaaag
     2401 tatggttggt catttttgtt caccatattg gctcctaata taggcgcttg ataattctta
     2461 atcgcttcct cgagataatc cacaccatag ttaaactcat gatttcccaa cacttgaaca
     2521 tcatagctca tttggttaat caagtctgta agctctttgg ctgaaccata ttctttagca
     2581 agataataac tcaaaggaga cccttggatg aaatcaccat tttcgatcgt aattaccggt
     2641 ccatctgctt ttgcccgtaa ttccttcagt ttcgacgata ctttagccgt tcctaaggcc
     2701 agatcggttc tgccggaata gtctgttggt gaaacatagc catgcatatc acttgtctct
     2761 aaaaacgtaa tgttcattct gcgtgctcct ttagctgact caaaatagaa aaaccgaagc
     2821 gtattcagct gttcggtctc tttttttgat ttataatgcc ttaatccttc atttgaagtg
     2881 tttttaaaat gttgcctgga ataaagtttt ctgttgtctg gcgaacaatc cttccttgat
     2941 cgagatgata aaggttatac ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat
     3001 cttcaccaac aaaggacata ctcgatacag taaaataggg gatcccatta atttcatcag
     3061 taaatgtgtg atgggtatga ccgtttagga tacagcgaat atcgtaaggt gcgattagct
     3121 tcaagaaacg ttcggtacca ggccattttg gtatactgct ttggtgagac agtagatgat
     3181 gatgggtgac caagaaaatc ggttggtctt tttcttgctc taaggctttt ttcagccatt
     3241 gaaattgctg atcgtccact ataccatctg cgtatccata gcttgaatta tcaaaggaaa
     3301 tcactgtaaa ctcaggatat ttgactatct ggttgtacgg ggcagaagag gcggcttcat
     3361 cgcaccagcc tacccaaaaa tgttctttga tatcgtgatt gcctagagta acaagaatcg
     3421 tcgtttctcc tagcgcattt tccagccaag tcttcaagta tcgataatcg tctatttcgc
     3481 catcttcggt taaatctccg ctgataatca caaggtccaa gggtgtttgc tgcaggagat
     3541 gatgaatgca ttgctccaga gggataaggg gattctgcat cgtagccagc attcccttat
     3601 agccttcttc acaagcctca tactctctgc gaaagtgtat atctgatata tgcaatatat
     3661 tcattcttat ctcaatctct ttcttataat tccggttatt tgatctaaaa taaacacggt
     3721 tacgactacg ccaatcaaaa taatactcac tttgctccaa gaccgagttt gaatcgcgaa
     3781 tatcaatgga gcaccgattc cgccagcgcc aactaagcct aaggtagccg cactacgaac
     3841 cgctatttca aaatgattta atgctaaaga acaataaatc ggcatcaaat aggggatgcg
     3901 aacaaagaaa atcgtttgcc aaaaatttgc tcctaccgca tgcatttctt caacaagtcg
     3961 ctcatccatc gcttccattt cttctgtaaa aagtttgcca agcattccga tctgatggac
     4021 gccaatcgcc ataacaccag caaaggggcc aggtccgacg actttcacaa aaataattgc
     4081 aaaaactagt tcaggaaaag ctcgtaaaac attacagata agcttgccaa tttttgttac
     4141 ccatggatgt gcttgccaga ggttaaccgc actgatcaag gtaatgggaa tcgctaatac
     4201 cgtagcgatc attgtcccta aacaggcgat tccaatggtt aataatagca aactaatcaa
     4261 atcctcgccg ctgccatcat atagaaactg ccaatcaggc tgacctaacc ctctcaaaac
     4321 gtccattccc atggaaggag agaaggtgct taatccagag taatccagag tagcggccga
     4381 caaaataacg agaatcacta aagcaactaa tgataaaagg gttcttctgt tcatttcacg
     4441 aagcatcata acagggacct cctgacacgt tcacttaatt tatcaacgat cgcgacagtt
     4501 actaggatcg ttagaataat gattgaaact cgttcataat tcaaaagcgc aagtgaggaa
     4561 tttagaataa cgccaatacc accagctccg acatatccaa ggacagtaga agcacgaaca
     4621 ttcacctcaa acgcgtaaaa agcatagctc gcaaactgac tcaaaacttg tggagcgatc
     4681 gaccaaatag cgatttgcat tttatttgct ccaacagccg cagcggcttc aatcgggcca
     4741 aagtcaatgg tttcaattgc ttgaaacatt aattgtgaca ccataccaaa agtaaagaca
     4801 gcgatagtca acacgccagt cgcttcacca attccaaaca cagcaacaaa aagcgcggct
     4861 aaaagcaggg tagggatggt ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga
     4921 agacggtttt ctgtcactac agtggtcgct aaaaaactaa cagggatcgc taaaattaag
     4981 ccaatgaccg ttccaagcaa agacatttga agtgttttca ccataggctc gactaattta
     5041 ggtaaatagc taaagtctgg atgaatgaag cgtgataaaa agacagtcat ttgattggag
     5101 ttttgaaaaa cctgagaaaa atcagctgac gtgatccagg cacttccttg aaaacaaaca
     5161 attaagaaca gaagaataag caggtgttta taccatttaa agtgaacggt ttctttcaag
     5221 ctcatcgatt tcgcctgcct tttcttcaaa gattgcattg ccataaattt gctctaagcg
     5281 ttcatcggtt aagtcgtctg gacgaccatc aaagacaacc tgagcagctt ttaacgcgag
     5341 cacacgcgta gaaaactcac gagccagagg gactgaatgc agattgacaa tgactgtttt
     5401 atccatcgtt tgattgatat tttttaggtc catcataacc ttttgagtcg tgattggatc
     5461 taatgatgca acgggttcat ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc
     5521 aatggatact cgctgttgtt gcccaccact gagctcatca ctgcgtgtct gcaatttatc
     5581 agacaaacca actttttcca gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag
     5641 acctaaaata cttttccaag tggagtaata gcccaaacgt ccagatagca catttttctg
     5701 aaccgtacta cgctttacca gattgaagct ttgcgagatt agaccaatct ggcggcgaat
     5761 ctgtcgcagc tcttttttat tcgcttttgt aatagaggtt ccattaatgc gaatatctcc
     5821 tttggtggtt tctgccaagc gattgatact gcgcagcaga gtgctctttc cagcaccact
     5881 caaccctatg atggaaacaa attctccatc ttcaattgtt aagtcaatat ccttcaagcc
     5941 aacaacgcca ttgggataga ccttcgaaac attgtcaaaa ataatcatta tccttctcca
     6001 tttttttaag tgtaagcaaa aaaaagcttg ctgacactta ctgatgtttt attctgctgc
     6061 ttttttcgtg tagtctctga tcgtgtcata ggcagaatca gcagcttctt catagccctg
     6121 atgcccccac aaagccattg cttctttgcc atctttatca tccttcatat ttaagaaggt
     6181 ctctttaatt ttcgctttta aatcttttga catgtcaggc tgtacagcga ttgcgtcatt
     6241 cggaatatcg ccttttgtca ggtacaggac ttttaaatcc ttaaataaat cattatctgg
     6301 gaaattcgaa gcaaaaacat ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt
     6361 gttcaacact gcggtaatct cactcggaat atcattcaca gtggtcatct ttgtatctgc
     6421 agtaggatca atcccagcat ctttcaattc agcgacagga tagatgtagc cgctagcaga
     6481 gttaggactt aaggttgcga tattttttcc ttttaaatca gttaacttat tcaatccgct
     6541 atcggcacga accaatactt ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa
     6601 gccagtttct tgatcataat cgccgagctg agaagtcagg attgcatctg cagctccagc
     6661 atcctttgct tgaacatagg ctgcaggcgg cataattccg acatcgactt gaccagaaga
     6721 catcgcttcg acgatcgttg aataatcagt agctaacgtc acttctacct cacgatctaa
     6781 ctttttcgat aaatacttag caaatggttt tgcttttgcc tccatactac catcattgtt
     6841 tgttggaacg aattgaacaa ctaatttttc tttgctctct ttttctgagc tgctacttgt
     6901 attcccagaa ccgcaggccg ccaaagtggt caataacatg actccgatga acccttttgc
     6961 taactgtttg aactgcattt ttctcctcct gttggatata atttctaata cgtctaaatc
     7021 atagcaggga atcaaaatca atgaagtgac gcacgtccta ttttacacaa cttttacata
     7081 caatttacaa acaattataa ataattattc gtattgagcc gaaaaacaat ctaagctata
     7141 ataaagaaaa acttcttaga ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat
     7201 taactgctta cgttgagaag cacaacctgc aggattatat ggattcagat atattggcaa
     7261 tggcttcttt actttttttt gaaaaggaag agcacctcat tcaaacggga aaaacctcag
     7321 attatttgta ttttttagtg gatgggacgg taatggtgta ctcgtattct tctgatacac
     7381 aaaatatttg catcgattac gcaaagcccg ctacaccact cggcgaagcc tcttcattgt
     7441 gggaattatc gccgaaaagc agcgttaaag cagtgactaa gtgcatctgt atcagcattc
     7501 cgctaaatca atatcgaaca cagcttcaaa aagatgttcg ctttttgcag aatatttgcc
     7561 agttactaag ttatcgttta aattccggga taaatttggc gaactcactg acagaacctg
     7621 ttgaaactcg tttagcaaag tttattttga cccatcacga aaacgaacgt ttctcatttc
     7681 ggttaaccac ctgcgcctca atcttaaatg tcagttatcg acatctttta cgaacaatca
     7741 cgaattttcg tgaggagaat attatcgaaa agaaaaagaa ttattacttg attcatgata
     7801 tcgaagcttt agaggactta gctgagaata taagtagtcc agtaaaagca gaataacagc
     7861 tataaaaaaa cagcagtcag aagtgattgc tggttttttg atagtggaga aattgggaaa
     7921 cggaaaatct tcgttgaaga gtagtgttct atatattggt atgctatact tttaaaagtt
     7981 caatgtgtca agacacataa aaaggggaga aaaattttga agaaaatact tgtttcattg
     8041 ttgtttttgg tgtcattagt aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa
     8101 agtgaaaaag agaaaagtaa aatcgctttg gttcttggat ttggcggggt tgatgaccgc
     8161 tccttcaatc aagctggctg ggaaggatta gaggcttggg gaaaagagaa aaagctgaca
     8221 gcaaaaaaag attttacgta tatagaaacg aacaatccag aggataacag taccaatatc
     8281 gatcaggcaa tcagaaacgg ctttaccacc attattagct tgagctactt gcaagcagag
     8341 gaactggaaa atgctgctaa agataatcca aagttgaaat ttgccttggt agatggtgag
     8401 attactggga aaaagaatat ctcatcagca acctttaaag atcaagaaag tgcgtattta
     8461 gcaggactag ctgccgcata tacgacaaaa agtgatcaag taggctttat tggtggaatg
     8521 gaagggcccg tgatccaacg tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc
     8581 acagcactga acaaggaaat tactgttaac gtacaatatg ctgcatccta tgattcgcta
     8641 gataaaggaa aagctattgc tgcttctatg tacaaagagg gagtcgatat tctttatcag
     8701 gcggcaggaa gtactggagc tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac
     8761 tatagcagaa aagtatgggt gattggtgta gatcgcgatc aagcggatga aggggattat
     8821 aaaatggaga acggcgagac agggaatttc acgttgactt ctacaaagaa aaatgtcggt
     8881 gcagaagtca aagaatttgc agaaacttct cataacgata aatttcctgg cggcaagcat
     8941 ttgacctttg gtttgaaaga agaaggggta gatctgacaa aaggacaatt atcagaggaa
     9001 gcctatcagg cagtttcaga cgcaaaagag aaaatcatca gtggtgattt cagactgtag
     9061 acaaagtcca ttttatggat gatgtctaca gt
//
LOCUS       DASJPH010000110         9061 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJPH010000110 DASJPH010000000
VERSION     DASJPH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.111"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="U3995_002469"
     CDS             complement(<1..641)
                     /locus_tag="U3995_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1797758.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="U3995_002470"
     CDS             complement(643..1407)
                     /locus_tag="U3995_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1797759.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="U3995_002471"
     CDS             complement(1415..2092)
                     /locus_tag="U3995_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1797760.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="U3995_002472"
     CDS             complement(2129..3514)
                     /locus_tag="U3995_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1797761.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="U3995_002473"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U3995_002473"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U3995_002474"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U3995_002474"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1797762.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="U3995_002475"
     CDS             4453..5310
                     /locus_tag="U3995_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797763.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="U3995_002476"
     CDS             complement(5391..5726)
                     /locus_tag="U3995_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797764.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="U3995_002477"
     CDS             complement(5793..7163)
                     /locus_tag="U3995_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1797765.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="U3995_002478"
     CDS             complement(7241..7777)
                     /locus_tag="U3995_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1797766.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..>9061
                     /locus_tag="U3995_002479"
     CDS             8086..>9061
                     /locus_tag="U3995_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797767.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFY"
BASE COUNT         2716 a         1876 c         1412 g         3057 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 t
//
LOCUS       DASJPH010000111         8865 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJPH010000111 DASJPH010000000
VERSION     DASJPH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.112"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1177)
                     /locus_tag="U3995_002480"
     CDS             complement(221..1177)
                     /locus_tag="U3995_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1797768.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="U3995_002481"
     CDS             1281..1883
                     /locus_tag="U3995_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1797769.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="U3995_002482"
     CDS             complement(1971..3470)
                     /locus_tag="U3995_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1797770.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="U3995_002483"
     CDS             complement(3486..4010)
                     /locus_tag="U3995_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1797771.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="U3995_002484"
     CDS             4308..5057
                     /locus_tag="U3995_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1797772.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="U3995_002485"
     CDS             5048..5833
                     /locus_tag="U3995_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1797773.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="U3995_002486"
     CDS             5802..6518
                     /locus_tag="U3995_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1797774.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7654)
                     /locus_tag="U3995_002487"
     CDS             complement(6620..7654)
                     /locus_tag="U3995_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1797775.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7658..8716)
                     /locus_tag="U3995_002488"
     CDS             complement(7658..8716)
                     /locus_tag="U3995_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1797776.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2667 a         1790 c         1588 g         2820 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cgactgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatt
//
LOCUS       DASJPH010000112         8494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJPH010000112 DASJPH010000000
VERSION     DASJPH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.113"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U3995_002489"
     CDS             complement(<1..173)
                     /locus_tag="U3995_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797777.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U3995_002490"
     CDS             complement(593..1714)
                     /locus_tag="U3995_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN1797778.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U3995_002491"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U3995_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN1797779.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U3995_002492"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U3995_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN1797780.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U3995_002493"
     CDS             complement(4304..5215)
                     /locus_tag="U3995_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1797781.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U3995_002494"
     CDS             complement(5232..6239)
                     /locus_tag="U3995_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1797782.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U3995_002495"
     CDS             complement(6236..8365)
                     /locus_tag="U3995_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1797783.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="U3995_002496"
     CDS             complement(8370..>8494)
                     /locus_tag="U3995_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1797784.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DASJPH010000113         8440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJPH010000113 DASJPH010000000
VERSION     DASJPH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.114"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..358
                     /locus_tag="U3995_002497"
     CDS             <1..358
                     /locus_tag="U3995_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1797785.1"
                     /translation="DQLAKNHNEVLFIIPPVNEKWSDYTGLSQEMLQGFAKKIKFQLN
                     SQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEENHITASKYHLDDAF
                     FSKSWQHQIPDKLQLK"
     gene            499..1581
                     /locus_tag="U3995_002498"
     CDS             499..1581
                     /locus_tag="U3995_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1797786.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1581..2384
                     /locus_tag="U3995_002499"
     CDS             1581..2384
                     /locus_tag="U3995_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1797787.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2454..3194
                     /locus_tag="U3995_002500"
     CDS             2454..3194
                     /locus_tag="U3995_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1797788.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3205..4209
                     /locus_tag="U3995_002501"
     CDS             3205..4209
                     /locus_tag="U3995_002501"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1797789.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4254..6098)
                     /locus_tag="U3995_002502"
     CDS             complement(4254..6098)
                     /locus_tag="U3995_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797790.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            6309..6827
                     /locus_tag="U3995_002503"
     CDS             6309..6827
                     /locus_tag="U3995_002503"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1797791.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6955..7638
                     /locus_tag="U3995_002504"
     CDS             6955..7638
                     /locus_tag="U3995_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1797792.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7947..8020
                     /locus_tag="U3995_002505"
     tRNA            7947..8020
                     /locus_tag="U3995_002505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7981..7983,aa:Arg,seq:tct)
     gene            8179..>8440
                     /locus_tag="U3995_002506"
     CDS             8179..>8440
                     /locus_tag="U3995_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1797793.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISI"
BASE COUNT         2804 a         1354 c         1687 g         2595 t
ORIGIN      
        1 ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa
       61 atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa
      121 atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac
      181 caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca
      241 acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga
      301 tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag
      361 aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata
      421 aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat
      481 agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct
      541 gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa
      601 ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct
      661 ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca
      721 aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc
      781 atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt
      841 ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag
      901 gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt
      961 cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta
     1021 ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat
     1081 ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta
     1141 ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta
     1201 gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta
     1261 ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg
     1321 aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct
     1381 ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc
     1441 attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat
     1501 ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa
     1561 aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag
     1621 cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa
     1681 tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca
     1741 tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg
     1801 cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat
     1861 tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag
     1921 tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga
     1981 gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag
     2041 attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca
     2101 tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg
     2161 atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta
     2221 cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt
     2281 tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac
     2341 aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat
     2401 tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat
     2461 taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt
     2521 tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat
     2581 tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg
     2641 atcatattgc tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc
     2701 cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga
     2761 cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc
     2821 cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg
     2881 tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt
     2941 tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa
     3001 atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta
     3061 acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa
     3121 atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag
     3181 tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga
     3241 atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg
     3301 ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa
     3361 atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc
     3421 ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg
     3481 gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg
     3541 ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt
     3601 tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc
     3661 gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca
     3721 aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa
     3781 aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta
     3841 gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat
     3901 cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc
     3961 aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca
     4021 gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct
     4081 gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc
     4141 agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta
     4201 ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca
     4261 ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc
     4321 tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt
     4381 tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca
     4441 ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt
     4501 cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc
     4561 aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag
     4621 gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc
     4681 acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt
     4741 tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc
     4801 tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca
     4861 taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg
     4921 tcggtattta agatgtgatc gattagattg tagctgataa taacgattag aatgataata
     4981 tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa
     5041 atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg
     5101 ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta
     5161 gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc
     5221 agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag
     5281 taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa
     5341 tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat
     5401 acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga
     5461 cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca
     5521 gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt
     5581 ccagttgttc ctagataaaa tttcggtggc agtccagcat agttctgata aggagatgag
     5641 actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag
     5701 agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga
     5761 tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt
     5821 tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg
     5881 tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt
     5941 atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt
     6001 cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg taaataaaaa
     6061 gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct ttctaaaact
     6121 tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata aaaaatgaga
     6181 taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat ttttacagct
     6241 ataatttgaa aaaatttatc gttattatat gattaagaag gattgaataa agaaagaagt
     6301 tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg ttgattatat
     6361 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
     6421 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
     6481 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
     6541 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
     6601 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
     6661 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggcg tttgcatgga
     6721 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
     6781 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
     6841 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
     6901 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
     6961 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
     7021 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
     7081 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
     7141 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
     7201 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
     7261 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
     7321 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     7381 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     7441 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     7501 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     7561 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     7621 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     7681 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     7741 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     7801 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     7861 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     7921 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     7981 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     8041 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     8101 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     8161 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     8221 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     8281 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     8341 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     8401 gcatctaaag aaatcacaag tatgctatta atatctattt
//
LOCUS       DASJPH010000114         8332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJPH010000114 DASJPH010000000
VERSION     DASJPH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.115"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            408..677
                     /gene="rpsN"
                     /locus_tag="U3995_002507"
     CDS             408..677
                     /gene="rpsN"
                     /locus_tag="U3995_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1797794.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            692..871
                     /gene="rpmF"
                     /locus_tag="U3995_002508"
     CDS             692..871
                     /gene="rpmF"
                     /locus_tag="U3995_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1797795.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            903..1280
                     /locus_tag="U3995_002509"
     CDS             903..1280
                     /locus_tag="U3995_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797796.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1301..1450
                     /gene="rpmG"
                     /locus_tag="U3995_002510"
     CDS             1301..1450
                     /gene="rpmG"
                     /locus_tag="U3995_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1797797.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1474..2457
                     /locus_tag="U3995_002511"
     CDS             1474..2457
                     /locus_tag="U3995_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1797798.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2487..2609
                     /locus_tag="U3995_002512"
     CDS             2487..2609
                     /locus_tag="U3995_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1797799.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2627..4150
                     /locus_tag="U3995_002513"
     CDS             2627..4150
                     /locus_tag="U3995_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1797800.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4174..4413
                     /locus_tag="U3995_002514"
     CDS             4174..4413
                     /locus_tag="U3995_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797801.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4606..5067)
                     /locus_tag="U3995_002515"
     CDS             complement(4606..5067)
                     /locus_tag="U3995_002515"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1797802.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5262..5480)
                     /locus_tag="U3995_002516"
     CDS             complement(5262..5480)
                     /locus_tag="U3995_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797803.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5598..7214
                     /locus_tag="U3995_002517"
     CDS             5598..7214
                     /locus_tag="U3995_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1797804.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7455..>8332)
                     /locus_tag="U3995_002518"
     CDS             complement(7455..>8332)
                     /locus_tag="U3995_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1797805.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2856 a         1281 c         1661 g         2534 t
ORIGIN      
        1 ttgaattagt tcacttaatg tctattctta taatataagg aataatcaaa gttttaaaaa
       61 attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat
      121 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
      181 aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc
      241 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      301 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      361 ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      421 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc
      481 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      541 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      601 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      661 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      721 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      781 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      841 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      901 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      961 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
     1021 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
     1081 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1141 acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa
     1201 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1261 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1321 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1381 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga
     1441 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1501 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1561 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1621 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1681 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1741 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1801 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1861 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1921 gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1981 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     2041 agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt
     2101 tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa
     2161 gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2221 ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2281 ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt
     2341 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg
     2401 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2461 taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2521 gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2581 aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2641 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2701 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2761 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2821 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2881 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2941 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     3001 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     3061 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3121 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3181 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3241 tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc
     3301 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3361 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3421 tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag
     3481 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3541 aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3601 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3661 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3721 ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa
     3781 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3841 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3901 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3961 aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc
     4021 agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca
     4081 taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat
     4141 ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4201 gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat
     4261 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4321 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4381 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4441 cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca
     4501 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4561 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4621 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4681 atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt
     4741 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4801 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4861 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt
     4921 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4981 acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt
     5041 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     5101 gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga
     5161 aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc
     5221 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5281 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc
     5341 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5401 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta
     5461 agatgacgat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5521 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5581 atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt
     5641 caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag gaaattgctg
     5701 tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt
     5761 ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac
     5821 gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg
     5881 ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta
     5941 aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt attatagtag
     6001 tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag
     6061 ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg
     6121 agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca
     6181 aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag
     6241 gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct
     6301 attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag
     6361 aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat
     6421 ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt
     6481 ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta
     6541 ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac
     6601 aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac
     6661 tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt
     6721 taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt
     6781 ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag
     6841 gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta tttgtattct
     6901 ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg
     6961 atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc
     7021 atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag
     7081 aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa
     7141 cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt
     7201 actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta
     7261 ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg
     7321 gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta
     7381 gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     7441 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     7501 agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct
     7561 cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta
     7621 ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt
     7681 tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct
     7741 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc
     7801 tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc
     7861 tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat
     7921 tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc
     7981 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt
     8041 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     8101 gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata
     8161 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     8221 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact
     8281 gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga aa
//
LOCUS       DASJPH010000115         8248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJPH010000115 DASJPH010000000
VERSION     DASJPH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.116"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="U3995_002519"
     CDS             234..1559
                     /locus_tag="U3995_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1797806.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="U3995_002520"
     CDS             1552..1902
                     /locus_tag="U3995_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797807.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="U3995_002521"
     CDS             1884..2069
                     /locus_tag="U3995_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797808.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="U3995_002522"
     CDS             complement(2973..3248)
                     /locus_tag="U3995_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN1797809.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="U3995_002523"
     CDS             complement(3220..4173)
                     /locus_tag="U3995_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1797810.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="U3995_002524"
     CDS             4785..6278
                     /locus_tag="U3995_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1797811.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="U3995_002525"
     CDS             6413..6709
                     /locus_tag="U3995_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1797812.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="U3995_002526"
     CDS             6810..7661
                     /locus_tag="U3995_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797813.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="U3995_002527"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="U3995_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DASJPH010000116         8154 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJPH010000116 DASJPH010000000
VERSION     DASJPH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.117"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(310..807)
                     /locus_tag="U3995_002528"
     CDS             complement(310..807)
                     /locus_tag="U3995_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1797814.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="U3995_002529"
     CDS             complement(847..1656)
                     /locus_tag="U3995_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1797815.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="U3995_002530"
     CDS             1990..2847
                     /locus_tag="U3995_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1797816.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="U3995_002531"
     CDS             2840..3742
                     /locus_tag="U3995_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1797817.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="U3995_002532"
     CDS             3775..4125
                     /locus_tag="U3995_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1797818.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4174..5097
                     /locus_tag="U3995_002533"
     CDS             4174..5097
                     /locus_tag="U3995_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1797819.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="U3995_002534"
     CDS             5175..6068
                     /locus_tag="U3995_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1797820.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="U3995_002535"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="U3995_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1797821.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="U3995_002536"
     CDS             6683..6871
                     /locus_tag="U3995_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1797822.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="U3995_002537"
     CDS             6884..7786
                     /locus_tag="U3995_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1797823.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="U3995_002538"
     CDS             7818..8060
                     /locus_tag="U3995_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1797824.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2827 a         1424 c         1592 g         2311 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaaga tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggtt
//
LOCUS       DASJPH010000117         7677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJPH010000117 DASJPH010000000
VERSION     DASJPH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.119"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..2758)
                     /locus_tag="U3995_002539"
     CDS             complement(56..2758)
                     /locus_tag="U3995_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1797825.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="U3995_002540"
     CDS             complement(2771..4060)
                     /locus_tag="U3995_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1797826.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="U3995_002541"
     CDS             4210..5256
                     /locus_tag="U3995_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1797827.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="U3995_002542"
     CDS             5258..7411
                     /locus_tag="U3995_002542"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1797828.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2158 a         1570 c         1401 g         2548 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctg
//
LOCUS       DASJPH010000118         7537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJPH010000118 DASJPH010000000
VERSION     DASJPH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.120"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="U3995_002543"
     CDS             join(107..335,335..1248)
                     /locus_tag="U3995_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1797829.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="U3995_002544"
     CDS             1483..1935
                     /locus_tag="U3995_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797830.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="U3995_002545"
     CDS             1949..2638
                     /locus_tag="U3995_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797831.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="U3995_002546"
     CDS             2763..5114
                     /locus_tag="U3995_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1797832.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="U3995_002547"
     CDS             5194..5589
                     /locus_tag="U3995_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797833.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="U3995_002548"
     CDS             5914..6531
                     /locus_tag="U3995_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1797834.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="U3995_002549"
     CDS             6572..6871
                     /locus_tag="U3995_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1797835.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="U3995_002550"
     CDS             6905..7072
                     /locus_tag="U3995_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1797836.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="U3995_002551"
     CDS             complement(7117..>7537)
                     /locus_tag="U3995_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1797837.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DASJPH010000119         7486 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJPH010000119 DASJPH010000000
VERSION     DASJPH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.121"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U3995_002552"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U3995_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN1797838.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="U3995_002553"
     CDS             complement(2944..3378)
                     /locus_tag="U3995_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797839.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="U3995_002554"
     CDS             complement(3395..4600)
                     /locus_tag="U3995_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1797840.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="U3995_002555"
     CDS             5294..5680
                     /locus_tag="U3995_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1797841.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="U3995_002556"
     CDS             5774..5965
                     /locus_tag="U3995_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797842.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="U3995_002557"
     CDS             6479..6811
                     /locus_tag="U3995_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1797843.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="U3995_002558"
     CDS             7107..7262
                     /locus_tag="U3995_002558"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1797844.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2481 a         1320 c         1082 g         2603 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga cacctt
//
LOCUS       DASJPH010000120         7471 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJPH010000120 DASJPH010000000
VERSION     DASJPH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.122"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1085
                     /locus_tag="U3995_002559"
     CDS             <1..1085
                     /locus_tag="U3995_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1797845.1"
                     /translation="INDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..2445)
                     /locus_tag="U3995_002560"
     CDS             complement(1303..2445)
                     /locus_tag="U3995_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1797846.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2450..2635)
                     /locus_tag="U3995_002561"
     CDS             complement(2450..2635)
                     /locus_tag="U3995_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1797847.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2989..3234)
                     /locus_tag="U3995_002562"
     CDS             complement(2989..3234)
                     /locus_tag="U3995_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797848.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3228..3629)
                     /locus_tag="U3995_002563"
     CDS             complement(3228..3629)
                     /locus_tag="U3995_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1797849.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="U3995_002564"
     CDS             complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="U3995_002564"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN1797850.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5647..7413)
                     /locus_tag="U3995_002565"
     CDS             complement(5647..7413)
                     /locus_tag="U3995_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1797851.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2395 a         1432 c         1029 g         2615 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
     2101 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
     2161 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
     2221 atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt gtgaatcaga
     2281 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
     2341 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
     2401 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
     2461 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
     2521 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
     2581 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
     2641 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
     2701 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
     2761 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc cttctttaca
     2821 gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac tgatagaatc
     2881 tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa ttccttaact
     2941 attagaagat tcttttaaac agaaaaagtg gaaaggggat ttttttaatt agttaatttt
     3001 aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac
     3061 ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata
     3121 agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa
     3181 gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta ccataggtca
     3241 taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc
     3301 atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata
     3361 atctttcttc ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt
     3421 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac
     3481 ctgccttcat ttgaatctgc caatggttcc tcattatcct ttatgttagc gtttcgtttt
     3541 cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt
     3601 atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc cctttccact
     3661 aatacaacaa tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat
     3721 ttatcattct ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga ttaggctagg
     3781 ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact gaaattgggt
     3841 attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttttct atctctagta
     3901 aagattcctt tcttatttcc ttgcactctt aaaatccctt gacttgttgc aaaatctgca
     3961 aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct
     4021 tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca
     4081 gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca tctttccatc
     4141 catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca
     4201 taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaaca
     4261 tctggcgttg attttagata agtaacgatt gttacaggtc ttttctgcgg atcaagttct
     4321 ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt
     4381 tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc
     4441 ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc
     4501 acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca
     4561 tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa
     4621 tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt
     4681 acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta
     4741 aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata
     4801 ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact aattttcata
     4861 ctatataaat aagcgtctag aggttcccaa aaaattggat tcttaagctg aatcattcct
     4921 ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt
     4981 tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc agttattctt
     5041 gtattaatag taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta
     5101 tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca
     5161 ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg
     5221 tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca
     5281 aatggtgtaa acccaccttt gtgttgagta ataaactttc cattcaaaaa aacttttgct
     5341 tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga
     5401 gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat
     5461 aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct
     5521 aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt
     5581 aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc
     5641 taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt
     5701 aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc
     5761 ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac
     5821 agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact
     5881 tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa
     5941 gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt
     6001 tttaattttt tcttcttgcg ataatccttt caattcttct tcaggtgaaa tgtatttttc
     6061 aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt
     6121 tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct
     6181 tttatatttt tctggtacaa taggccaaat atcttgttta tctttaacta atgtaattgc
     6241 ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc
     6301 ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt
     6361 taaccctaaa attcttttta aggcatctaa taaccgatca tcagtgataa ttcctttacg
     6421 atagccttcc atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc
     6481 acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc
     6541 taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa
     6601 gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg
     6661 attaaaatgt tttgaataag ctggcatatg gatatgacct gccataattg aaggtaatcc
     6721 agcttctatt aagcctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa
     6781 actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg
     6841 tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc
     6901 taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt
     6961 tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat
     7021 cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac
     7081 ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc
     7141 agcaattaat agaggaattt tactttctgt ttgtaagata tagttttgat catatacctc
     7201 atcagcagtt cctggattat atcgtacagc tccaatatgg tactgattga ccatattact
     7261 caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc
     7321 caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata
     7381 tggttttttt cgtaaatcta cttttatagt cattttcttt ctcctctact tattagtaag
     7441 atttttccaa aaagtatcta tatatttttg t
//
LOCUS       DASJPH010000121         7285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJPH010000121 DASJPH010000000
VERSION     DASJPH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.123"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..818)
                     /gene="dltD"
                     /locus_tag="U3995_002566"
     CDS             complement(<1..818)
                     /gene="dltD"
                     /locus_tag="U3995_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1797852.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYT"
     gene            complement(821..1054)
                     /gene="dltC"
                     /locus_tag="U3995_002567"
     CDS             complement(821..1054)
                     /gene="dltC"
                     /locus_tag="U3995_002567"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1797853.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(1099..2304)
                     /gene="dltB"
                     /locus_tag="U3995_002568"
     CDS             complement(1099..2304)
                     /gene="dltB"
                     /locus_tag="U3995_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1797854.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2301..3815)
                     /gene="dltA"
                     /locus_tag="U3995_002569"
     CDS             complement(2301..3815)
                     /gene="dltA"
                     /locus_tag="U3995_002569"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1797855.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(3826..3975)
                     /locus_tag="U3995_002570"
     CDS             complement(3826..3975)
                     /locus_tag="U3995_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1797856.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            complement(4256..6298)
                     /locus_tag="U3995_002571"
     CDS             complement(4256..6298)
                     /locus_tag="U3995_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1797857.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(6288..7082)
                     /locus_tag="U3995_002572"
     CDS             complement(6288..7082)
                     /locus_tag="U3995_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1797858.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
BASE COUNT         2321 a         1472 c         1170 g         2322 t
ORIGIN      
        1 tgcgtataaa aatcgttttt caacccgaat ggattattcg tcgtttctcg ttctccaatt
       61 tgatttgcta aaatggataa ttctgtttct tggtattgtt ttggtaatcg ttttgactca
      121 tgatcgattt tttgctgtcg atctttcaac ccaatattgc tgaatacttc atcctctctc
      181 ttcagcatgt tccattggct gcgcagctga ttcaaagatt caggtaaagg tagcttccct
      241 tttttgatcg tttgcagaat cgccgtcaac gtatcattct cttttacttt tgagaaagtt
      301 aataatcttc tagctaaata gcgatcagca ggcgttactt tcttcattga aaacaaccag
      361 tcgtatgttt gaagttcaga ataatacgta ttaaagtaat cagtttttac accattttta
      421 acgaaccact gaggagaaat aataaagacg actttcttgt ttttcatagc atctcctgca
      481 gaacgcatca tcatatattg gcttaacgat tgtgtcccgg gtgctcccag caaaaatgga
      541 cgatagtttc gctggtattt ttctgccaga acagacggat gaaatggact gattctgctt
      601 aattcacttg agccaaaaaa aggaatatat tgtttttcag ataatgcata attttttatc
      661 gcatctcctt tcaacacgtt cactgacatg gaagaagagg cacgtctaat cgtttctgga
      721 ttagagatat ctacttgata ggaggaggta aaaaacacaa acagtaagcc aacagctaaa
      781 acaaccgggc cgaaaatagc aaaaagtttc tttttcatac tcaacccttc aatgcagttg
      841 cttgttgaat gattttattc ggtgtgcacc aatcctctcg ctcgaattca gatacaggga
      901 cttggatatc cagctctccg tctagctcga ctaataattg aacagatgct aaagaatcaa
      961 gcaacccttc atcaaataga tcaatatcac gattttttct tacttcatct gttcctgtta
     1021 tttcttctaa gattgataaa atttgttctt ccattttcct tttctccttt gttatataaa
     1081 ttaatatgtc actattcgtt agctaaaaaa aagtttatct aaaaatcctg aaaaaatcag
     1141 gaagctaaaa catactgctt gaaatgtcaa aaatacggct aagccatgcg taaatttgtt
     1201 agaaggcaac tgctctttat gcttcttctt aaatcggatc catgcatctg tcagacagat
     1261 cagacaagca tgatacagtc catatacgat gtagtaccac gtcaatccat gccatatacc
     1321 cattagaaga aacaaagcaa aataaccgat atttgacatt acgatcttgc ttttaaaaaa
     1381 ttttttcttc atcatgaaga acatcaatcg catatataca taatcacgga accaaaagga
     1441 cagcgtcata tgccaacgat tccagaattc tttgatattc catgataaaa aaggtttatt
     1501 gaaattgatt ggtgtctgat atcccatcat gtagcttgta ccgactgcaa ataagctata
     1561 gccagcaaaa tcaaaaaaca aatacatact gtacacgtac atataaccta ctaatgccca
     1621 tgaaatacca ccatgctgta aggcaatgcg tcccactaca ggaagcagta cttgaccaaa
     1681 ataataaccg ataatgaatt tatataaaaa accgacaaaa atattttgga cgccttttcc
     1741 caaaagatca acatatttat cacgaacagg cggatggagc tgatcttttt caaatctcct
     1801 atagcgatcg attggtccag aagagattgt tggaaagaac agcagaaact ggatataacg
     1861 aaatggctta tatgttttga tcatgccatc tcttatttcc atgatcattt gtacagattt
     1921 aaaggttaaa taagagattc ctaaaaaacc aagtaacgat gcttttccat gttcaaaaaa
     1981 aggagtcact ttcgttaaaa acagtggaat tatcgctaaa ataacggcgg caaaaaacca
     2041 tcctgtctga tttttccttt gccgataaac aaaatagccc catacaagta tcgtctgcca
     2101 caaaacatac ccaatcagcg ccgctccctg ttgccaataa ttacctccaa agctgacaaa
     2161 taaaaagaaa agtgtcactg ctgattgata aactggtaat cttttttccc atatcaataa
     2221 agatagaacg atcggtacaa gcgcaatggc taaaagaaaa aaataaaacg gctctgcata
     2281 agggatcata tgaggtaaca tcattcagga ttcacctcag ctgttaattt ttttcgatcg
     2341 atttttccat taactgtctg aggcagcaga tcgacataat ggaacttctg aggaatcata
     2401 taatccatta caacttctag taattgttct ttgatagatt tagtcaactg aaaatccggc
     2461 tgtttcgttt tgttttctaa tacgacaaaa gcaaccagct gctgtacttt tgttccttgg
     2521 tattttggta cgacgacagc ttgtttgatt cggttgtctt ggagcagata atgttcgata
     2581 tccccgagtt caatccggta tccatgtaat ttgacctgct gatccatacg tccttcataa
     2641 aataaaagcc catattcatc tagtctacct gcatcccctg tatggtaact tgctaagtca
     2701 ttttgacaaa agaacgcagc agctgtcttt tcgggattgt tcaggtaacc ttttgatacg
     2761 cttggtcctg caataatgat ttcacccgtt tctccttttg aaacaggttt accttcttgg
     2821 acaatagaaa ctttcgtgtc tttttttacg tagccaatcg gtagtcggtc ataactatct
     2881 agtacatgct ggtcaatctg aatacttgaa atagctacag ttgcttctgt tggaccgtaa
     2941 gtattatata tccgtgcatt tgggaatcga tcaagtagac tttctgcagt ttttttcgtc
     3001 aattcttctc cgcaaaataa aaacatgtcc aattccggca cattttctcc attgaatgta
     3061 ggttccatca agcaaatatc cataaatgat ggagtagaaa cccacacatt caacttcaag
     3121 gctggcaatg tcgcgaataa ttgacgaaag tcttgaacaa ctgctttatt taacggcttc
     3181 agcattccgc cagaaactaa tgcaggataa atagaaaata ccgataaatc aaaagaaaat
     3241 ggcgcttggg ctaagaaatg cataccaggc ttgatcccaa agtcttccat cgtccattga
     3301 acaaagctga ctagattatc atgactgatt tgtacgcctt ttggttctcc agtagtacca
     3361 gaagtaaaaa taatataaaa attttctgta ccttttacgc tatttctggg ataaaatcct
     3421 actggtatct tcttgatttc ttgaaattct ttttttgtta tgacaggaac cgaaggaata
     3481 tcttctatcc agtccccgat actgatgacc atagatggtt tagccacccg aaagatcgat
     3541 aggatacgct ccttgggtgt atgctcttca atcggcaaat aggcatggcc agatttgaca
     3601 acccctagaa atgagacaat catttcaaac tccaaattgc caaaaacgac aattggtcca
     3661 tcgccttcta cctttttttc taaataataa gcaagtgcgt ctgatgcttg tttcaattca
     3721 ccaaaagtat gcgtctcatc cgcctgataa gcaactcgat caggttcatt tattccccaa
     3781 tcatcgattg cttcaataat cgttttgatt tccatactct tcctcctaaa attcgttata
     3841 gataaaagtt cctccttgaa tatttttata gtgatacaga taaatcagga acagcagtac
     3901 tgcactataa aaaattgttc gaatgatgaa ttttccccaa taattcaatt ttggattaga
     3961 acgccatttg ttcattggat tccctcctca tataacctca cacaaagtgt aactaatttt
     4021 gacaaaaaga aacatgtgtc tagcttacag aaaccgtttt tttactaaca gttttgtcac
     4081 atagctttta taaaaatagc acatcaaatc actgcttttt tctttataat ttctcgtact
     4141 tttaaaaaat ataaaaaata aagaggttgt gacaaaaatc gtgtcacaac ctcttattcc
     4201 aaataaatgg tattcaaaat ttagttctat ttattatctt gtcttattcc gtatatcatt
     4261 cctcgataat ccgacaatat aactgactcg taatgacata aagcaaaaga tagataagga
     4321 tcaataagcc actcacaaga taagccaacc agtaatcggc agtttttata tactgagtga
     4381 agacattgat tgcaaatact gcgtgagtga tccctaatat catgggtgga aagaaggtaa
     4441 gtgcgttttg ctcgaagatc atccgtctga tccttctttg tggaatcccc agcttagtca
     4501 ataggtgata attttctttt tcttcctctg cttctgctaa ttgccggact gtcaaaatac
     4561 taccagtagc aataatcacg atcatcccga caaatagtgt gacaaaaata ttgataccga
     4621 ttcgccgatt aactgcacgc aatttcgaat agctggaaga gaaattcaaa cgagagcttt
     4681 cttggctaaa ttgtgcttcc ttcatttggt catccggaag tttctttgtg ctaatttctc
     4741 cttgtagact tccatttacg atatcataag aataataaac agcattgagc cactttatcg
     4801 acaggtcatt gctcagtcgt tcatctattt tttcattatt tccacctgat acatctacat
     4861 tgactacttg ataatccaca ccttttgctt tttcaaaaac ggtatcatct acaatcagta
     4921 caggacttag atataccatt gtttcgtcac ctaatacact tggaagcatt tgttgaatct
     4981 tcaattcttg attatctggc agtccaatac tagatccata taatgaaata ttccgaaaaa
     5041 gagtttgcat ttgatcaagc aaaacagtat gaccaggtga tttcaaagaa atgttcggca
     5101 aatctggatt aacttttctg aaggcttgat attgtgtttc cgtgatcaga ttagcagttt
     5161 gaaacatagg ttcttcgaga ttttgactat agatactccc aatgacttta taatgcaagg
     5221 tgatttcttt agttatcttc tgcccttcct ctgctagaat cggcttgatt tttttctctg
     5281 tctcaacatc aaactgatag tccatcgggg aggaaacttt ctcctggtag gataaaatag
     5341 tagagatact agccgtgccg ccaattgtca ttaaagaaat tccgagaatg actgtaatca
     5401 gagaatttgt acgccaactt ttgagcaaat gaacttgcgt gttacccatt atcaaaaaat
     5461 tgataccata gtaataaatc ttccatttac tcaatatgat caaaaataac cgaaatgtat
     5521 aacagaaaaa caaataagta ccgaccacac aaacgaaaaa aattaaaaaa ggaaaaaaga
     5581 aaactaatgt atcaagataa tgataactga tcatccatga agtgatttcc ctaaaatgac
     5641 tagctccaaa atatccgctc gttaaaagta gtaacccgat gactgcgagt aatcggtgcc
     5701 ttgtcctgaa gcgcattgtt gaactatctg cttgttcttt ttctccaaaa cttcgaatca
     5761 ttggatagcg ccatattttc catattgaat atacagatac tgctaaaagg atgatgaaaa
     5821 aagcaaataa agtatctact actgatggaa tggagataaa aaatggacta gtaagatcca
     5881 tatccatcat tcgaaccaaa atcattgaaa agagtttgga aaaaataata ccgagtaata
     5941 tcccagaaat acaggcgaac aatccaatta tcatgatttc taacacatat atacctgata
     6001 tctgcagttt attcatacca aaaagctgat agatgcctat ttccttttgc cttctattca
     6061 agaaaaatcg gttggcactg aatacgaaaa aaagcaaaac aacagtaatg ataaatccac
     6121 caaaactcaa tccaccatct atcgaagtat cttgacttgc tcttctgact aaagattgat
     6181 cgtaggtcat tgcgctgaag gaataataga tcattacgga taaagtcaaa ctgaaaaaat
     6241 aaacaagata gcctcgtgac tgccgaaaaa aacttttaac ggctagttta tacagcattc
     6301 atttttcctc ctccgccaat cgtcgcctgc atgtctatta ctttctcaaa gaactctctt
     6361 cttgtccctc ttctgacaac ttcagaaaaa atcacaccat ctttaataaa taaaattcta
     6421 tcgcagtagc ttgctgtata tggatcatgg gtgactaaca gtatcgttgc tttttctttt
     6481 tgattcatgg tcttcaagta gttcaacaat tctgtagccg acttcgagtc caaagagcca
     6541 gttggttcat ctgcaaaaat cacttgtggc tgaacaacaa gtgctctagc agctgcaatt
     6601 cgttgtctct gtccgacaga aagttcatcg ggataccgtt tgagcagttc ttctatattc
     6661 aacaattgag ctacatgtct tactctcttt tccatttcct ctttagtcat ccgttcaact
     6721 gctaggggca ataggatatt gtcttttgca tttagggtat cgatcaaatt gaactcttga
     6781 aaaatgaatc ctagtttttt tcgacgaaag tcactgagct cactgttttt catttttgtg
     6841 atttcttttc cttggatcga gacactgccc atcgtaggca aattgatcgt ggacaagata
     6901 ttcatcagcg ttgtctttcc tgcaccactt gggcccataa tcccaacaaa ctcgccttct
     6961 tctactgtga atgagatatt atctaaggct ttgacgggag aaagtttgct gccgtagctt
     7021 ttacttagat gtttcacttc gatcatcttt tccaattctt ctcctcctct ttcatttttc
     7081 atttctattt atttgcatag tagcatatct acgaataatt tgctcttatg tttttgtcac
     7141 ttttacttgg actgaataaa aaattttatt caattatttt attcagcaaa attaaatatg
     7201 caaaaaacaa atccaccagc tacaagtttt cacctttttt cacaataaaa tatacactac
     7261 atatagtata gaaatccaat tatac
//
LOCUS       DASJPH010000122         7218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJPH010000122 DASJPH010000000
VERSION     DASJPH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.124"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="U3995_002573"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="U3995_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1797859.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /locus_tag="U3995_002574"
     CDS             complement(920..1678)
                     /locus_tag="U3995_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1797860.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="U3995_002575"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="U3995_002575"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1797861.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="U3995_002576"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="U3995_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1797862.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="U3995_002577"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="U3995_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1797863.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="U3995_002578"
     CDS             complement(4434..5291)
                     /locus_tag="U3995_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1797864.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="U3995_002579"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="U3995_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1797865.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2281 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttatttat
//
LOCUS       DASJPH010000123         6937 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJPH010000123 DASJPH010000000
VERSION     DASJPH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.125"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            103..993
                     /locus_tag="U3995_002580"
     CDS             103..993
                     /locus_tag="U3995_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1797866.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1066..1890)
                     /gene="nadE"
                     /locus_tag="U3995_002581"
     CDS             complement(1066..1890)
                     /gene="nadE"
                     /locus_tag="U3995_002581"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1797867.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1902..3374)
                     /locus_tag="U3995_002582"
     CDS             complement(1902..3374)
                     /locus_tag="U3995_002582"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1797868.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(4011..4373)
                     /locus_tag="U3995_002583"
     CDS             complement(4011..4373)
                     /locus_tag="U3995_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1797869.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4596..5348
                     /locus_tag="U3995_002584"
     CDS             4596..5348
                     /locus_tag="U3995_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1797870.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5488..6411)
                     /locus_tag="U3995_002585"
     CDS             complement(5488..6411)
                     /locus_tag="U3995_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1797871.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6426..6698)
                     /locus_tag="U3995_002586"
     CDS             complement(6426..6698)
                     /locus_tag="U3995_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797872.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2149 a         1363 c         1165 g         2260 t
ORIGIN      
        1 aattaaccaa aatattaata atgattaaaa caaataattt aatataactt ttattaacaa
       61 atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac
      121 gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag
      181 ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt
      241 gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa
      301 gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatattag ttttccagaa
      361 aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat
      421 tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat
      481 gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc
      541 ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg
      601 gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat
      661 atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct
      721 gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg
      781 ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac
      841 atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa
      901 gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat
      961 caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca
     1021 aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc
     1081 atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat
     1141 tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata
     1201 tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt
     1261 cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg
     1321 tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa
     1381 gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc
     1441 agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc
     1501 gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat
     1561 atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc
     1621 atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt
     1681 ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg
     1741 gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt
     1801 cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa
     1861 ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt
     1921 tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta
     1981 gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa
     2041 tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt
     2101 tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta
     2161 ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca
     2221 tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac
     2281 acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc
     2341 atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc
     2401 tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc
     2461 atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc
     2521 gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca
     2581 ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc
     2641 gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta
     2701 tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa
     2761 tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt
     2821 gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt
     2881 gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct
     2941 gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat
     3001 tggcagtgcg ctaaaggccc ttccacttgg atcaatggtt catttgcaaa aactagttct
     3061 ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa
     3121 ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga
     3181 ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa
     3241 ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag
     3301 tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg
     3361 taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa
     3421 caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg
     3481 gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta
     3541 acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg
     3601 ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta
     3661 agataccgat gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag
     3721 tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata
     3781 acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg
     3841 aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct
     3901 gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg
     3961 tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa
     4021 tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt
     4081 ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt
     4141 tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg
     4201 atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac
     4261 gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac tactttagga
     4321 taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt catctcattc
     4381 ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc tttcctagca
     4441 ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt tatcacttaa
     4501 tagtataaca taatttcatg agaaaaaaat acaatttttt cttgaccatc tcgttacgta
     4561 aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata aagaaaatgt
     4621 cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt ggactgctaa
     4681 aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa gtcgatcgtc
     4741 tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc caagaggtct
     4801 tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa atgttgctgg
     4861 aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt gatatgtacg
     4921 aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa caaaagttga
     4981 aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa aaaaaggtta
     5041 cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa atgcaaactg
     5101 ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa gatgaaagct
     5161 tagcagaccg tatcttccaa gctcataaga agtggttatc ttacagctgg cctgaatatc
     5221 agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg ttcactgcat
     5281 attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt atccaaaaaa
     5341 aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct aataacaaaa
     5401 cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca ttctttcaca
     5461 agttttttca tcctacttta aatatattca tcgttgaata aacgtataag gtattttcac
     5521 tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag cttccagatg
     5581 gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc gtccaatcac
     5641 cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca tatccgaatt
     5701 tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt ttcctttctc
     5761 tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa gttgatgaca
     5821 tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca acgtgatgcc
     5881 caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg attgttctcg
     5941 atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa ctagaggtcc
     6001 atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa tgatcaatcc
     6061 atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt tctgatagcc
     6121 cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga tcccactgat
     6181 caactgatca aaatacggat gattggtata aggcagaatc actccaatgc ttttcgtttt
     6241 tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt tcatgaccgc
     6301 aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta cacgtgaaat
     6361 agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca ctgctgcttc
     6421 gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc gcattcaaaa
     6481 aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca atatatatct
     6541 tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct attcaaccct
     6601 tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata aaaataattg
     6661 ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc ttttgatact
     6721 ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca aagaaaagtt
     6781 ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat tataatgata
     6841 ctgtaaatat atctttatta ttagtgaatg gatatttact tttaatagat aaaatattat
     6901 aactattaaa aataactatt ttcattataa ttacaca
//
LOCUS       DASJPH010000124         6666 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJPH010000124 DASJPH010000000
VERSION     DASJPH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.126"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            534..1124
                     /locus_tag="U3995_002587"
     CDS             534..1124
                     /locus_tag="U3995_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEN1797873.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1151..2461
                     /locus_tag="U3995_002588"
     CDS             1151..2461
                     /locus_tag="U3995_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1797874.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2458..3177
                     /locus_tag="U3995_002589"
     CDS             2458..3177
                     /locus_tag="U3995_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1797875.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3192..4277
                     /locus_tag="U3995_002590"
     CDS             3192..4277
                     /locus_tag="U3995_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1797876.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4413..5945
                     /locus_tag="U3995_002591"
     CDS             4413..5945
                     /locus_tag="U3995_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1797877.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6123..6344
                     /locus_tag="U3995_002592"
     CDS             6123..6344
                     /locus_tag="U3995_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN1797878.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2335 a          721 c         1279 g         2331 t
ORIGIN      
        1 gttagatatg ctagttttaa taaagaattt gatttaaact cctatttgtc taagatactt
       61 atgaacgatg agttgattgc ctgtattcac tcatacccat ataaacaaaa tccaattaaa
      121 caacgaatat ttaactattt tttactaaac caatccatta ttggggttaa aacattagca
      181 tatttaagcg gaatgggtag aaataggaga ttttaatcat aaaaaactgt actatagtga
      241 atttttggag atttatattc cagttatata ttaatgattg tgatacttgt actattgtaa
      301 aaattcgtta ggtttaaata actggaaata tcggaaaatg ggcattatac atcacggaca
      361 tcccttcttt tggaaaaaat gttacgattt ttataagtaa tattgtatcc ggtggaatca
      421 acgatgaaga taacatatcc atcgttgtag attcagtagc attgaatgat gctcttgttg
      481 caagaaatcc gaataaaatt gttaagtata aattgaatta ataggagata tgaatggaaa
      541 ttagcagtaa agaatattta aaagcggttc ttcgtatgga aaagaatcta tatatagaat
      601 ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc acatccggac tacaacgctt
      661 ggcggtatac tcgtcgaatg agaattacat cttattatta ttcaaagaga aagaaaaata
      721 tcatttatgc gattatgtat attgtgtctt gcagaagaat gaatagtttg ggaagaaaat
      781 gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt aagattttac catacacaag
      841 gaattgttat taatggtaac gccataatag gaaaaaattg ttacttatat gggaataact
      901 gtattggaaa tgatggaatc gatcctaagt gtcctgtaat tggaaataat gtacgtgtat
      961 gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa caatgtagtt gttgcggcag
     1021 gagctattgt tatcaaagat tgtctagaga atggtgcaat tcttgcaggt gtaccggcaa
     1081 aaataatagg gtatgttaat ttaacaaaag tatttttaga ataattttta gcgaaagaag
     1141 gtgaataacg atgcgtattt caataaaaag atggtcagga gcagattgga cattgttcac
     1201 gttggcattt tttgcaatct ccatagttct tgtatatact tttaacgccc caagagcaat
     1261 tttatatttg ggagatataa ttaatatctt tgtgttatta aatgcactta gctttagacg
     1321 cagaaaagga atatatgcta gcaaaaatga gatgctgata gttttgtttt tactagtttc
     1381 tatttgtagc tcagttatta atttagaaaa tataggattg attatctggg ggatacgaaa
     1441 taacactcgt atgttcatag tatatataaa ttgtgcaaca tatttaacaa ttaaagatta
     1501 ctataaagta ttcgctataa ttgaaaaatt attctggatt tcactgccac tttgtttaat
     1561 tgaaagattc atggtcacct atccaactgg aacaattgta ggagatatga taggtggagt
     1621 tttttggaat tattcaggtt gtaatatgcc attgaattta attttgtgtg tgacagttac
     1681 tgttgttaca atcggatatt tcaagggaga aaaaaatttc atccatttta ttttatgtgt
     1741 ttgtgcagca ctttttatgt ctgcattagc tgaattgaaa gtttttattc ctgaattatt
     1801 tataatattt gttgcaatag tattcacttt aaaagttgat tttaaagtag tgatcaagat
     1861 attatcatgc gtaatacttg tgtcgatagt attatccttt tttataacaa tatttattac
     1921 attgaacgat aatggacaaa gctatgctga taatttcaca attcaaggtc ttttagaata
     1981 tgcgtcaaga gattctggat atgatggtgt tggtgattta aatcgaataa ctggtatctc
     2041 tagagtagta gatgaaatat ttaataataa ttttagatta gtcctaatgg gagtaggcat
     2101 tggcaatgca gagtttacca atttttttgt cagtgagttt tataaatcgt ttggtaatct
     2161 tcattatcag tattttcatc atatttggtt atttatagaa gttggaatat taggagtttc
     2221 gatattccta ttttttataa taaagacatt tttcaatgca aaaagatctt ttatgaagga
     2281 taaagatgca agtcttgtta aagttattgc aattatgtcg gtattctttt ttgtgtataa
     2341 cacaacactt aggggtgaaa caacggggcc actgatttat atgattttag ccatgcctga
     2401 agttgtgaaa aggaaagagc attattatag tgaaaaaaat acaaaaggag agatattatg
     2461 attcctaaga agatacatta ctgttggttc gggggaaatc cacttccgaa aaaagataga
     2521 aagtgcatag aaagctggaa aaagtattgc cctgattatg agattattga atggaatgaa
     2581 tcaaattatg atttaaataa gaaccgttat atggccgatg catataaaga acgtaaatgg
     2641 ggttttgttc cagattatgc aagatttgat attatttata atcatggtgg attttattta
     2701 gatacagatg ttgagctaat taagtcgtta gatatacttc gtgaaaatca tgcctatatg
     2761 ggatttgaag gggaaatctg ggttaatggc ggtattggtt ttggtgcaga agaaggacat
     2821 tctgttatta aaggtttaag ggatatttac gattcattaa gtttttatca aaatgatggt
     2881 acattgaact tgaaaccgag tccgtattat attacagatt atcttgaatc tattggattg
     2941 gttcgcgata acagcgaaca aataataaat ggcctgcata tctatcctac ggattatttt
     3001 gctgccaagg attatgatac tggaaaaatt tataaaacta gtaatacaat aagcattcat
     3061 caatataatg catcttggaa aacacctaaa caaaaatttg tttatagatt aaaatcaaca
     3121 attggacaaa aaaaatttaa caggttactt gcactgaaaa aatttttaca cttataaagg
     3181 agaaaagtac tttgaagaaa atttgtttca ttagtggtgt aatttctcgt tctgggggaa
     3241 ctgaaagagt agggagtatt attgccaata gcttgtctga tttaggttat gatgtcacta
     3301 ttttaagctt ttggaatagt ggagagcctt attttgggtt agataagacg attaaggttg
     3361 attatatgtt aaatcctaaa acggagggaa aattatatag aacttattta tatccggttt
     3421 ggaaattgca caagtatata tgcaaaaatg aatttgatat agtaattgat attgatactt
     3481 tgttgtcttt gtatactaca agggcaatta aaggaacaaa gtgcaaattg atttcttggg
     3541 aacattttaa ttactggaca atggaaatgc tgaaagaaaa gaagagattc aaggctaaaa
     3601 aattgataaa aaaatatgca aagaggcttg tggttctcac agaagaagat cgtcaaaaac
     3661 atattgatga atatagttta tcagaagatt ttgttataac tatgccaaat ccgtgtatat
     3721 ctaatatcga gtacgaatat cattatgata aaaagagatt tcttgcagta gggcgattag
     3781 tggaagagaa aaattttgct gacatgataa gtgcttggaa aattgtagaa aaaaaagtag
     3841 atgattggga tcttatcatt gtaggaaaag gtgaattaga aaaagatctt cgccagcaga
     3901 taaaagattt aaatctgcaa agagtaacta tagcagggca ttcagaccat gtggaggaat
     3961 attataaatc tgcatcttgt tatgtgctta gttctgttta tgaaggattt cctatggtta
     4021 tattagaagc acagtcttat ggattaccag ttatttctta tgattgtaaa actggtccga
     4081 gagatttggt acatcataat tttaatggga tgttggttga agataagaat atagaccaat
     4141 tagctaagag catgataatg tttactaaga atactgattt agcaatgaga atgtcactga
     4201 acgcatataa taatgtacaa aaatttaatt tgaaagaaat tacaaaacaa tgggttgctt
     4261 tgattgaaaa tgtatagttt tttgtggttg tccaaacatg aaaattagcg taattaatca
     4321 aagagctaga agaaacctat tacatttcaa gattttagaa attatatagt attaggtaac
     4381 aataaaaaat agaaagtttg gagccatatg atatgataat taatcgtaaa caaaatgcta
     4441 aaaggaatat aattactgga acaattttta agttatatca gattatcatt ccctttttta
     4501 tgcgtacact tatgatttat cagatgggaa tggagtatgc tggattgaac aacctgttca
     4561 catctatatt tcaaatgtta aatttagcgg aattgggaat aggtgctgca ctaactgtaa
     4621 gtatgtataa acctatagca gaagacaata gaaaagagat ttgtgcttta ctgaacgcat
     4681 ataaaaagta cttttcaatt attggatgga tagtgtttac attaggattg ttatgcattc
     4741 cttttttgag atttttagtt aaaggttcga ttcctggaaa tttaaatatg acagttttgt
     4801 atttaatgta tttgataaat actgttttgt cgtattggtt attttcatat agaaagagtc
     4861 taatatatgc acatcaaaga aatgatgtaa ttaacgtgtc tatgctaatt acttttacat
     4921 tccaatttgt aatgcaggca tatgttctga ttttcttaaa aaattactat ttgtatttat
     4981 tggcttctat tttgggacaa atagttctta acttaatgtg tgctgttgtt tctcaaaaga
     5041 tgtatcctga ttatactcct gttggtgagt tagagaaaga aactattgac gatattataa
     5101 aaaaagtaaa aggattagtt acaaacaagg taggcggtac aatattacga tcttcagatt
     5161 caattattat ttcatctttc ttaggtcttt cagtacttgc gatttatcag aattattact
     5221 ttattatttc tgccataatg agtatatttg caataattta tgaatcatgt atagcaggta
     5281 taggtaacag tcttattgta gaaagtcaag aaaaaaatta tatagatttt tgtgatatta
     5341 caacaattat aggttggttt ctggcaatta gttgtgcatg ctttattaca ttatttcagc
     5401 cgttcatgat tatctgggtg ggaagtgata atcttatgag tatttcattt gttatattta
     5461 tgatagttca ttttttctta tatgaaattg atcagttaat tgggttatac aaagatgctg
     5521 ctggaatatg gtacgcagac aggtttagac cattaattac agcaatgata aacattgtat
     5581 taaatatcat cctcgttaat tggttcggat tatatggagt tttggcggca accattgttt
     5641 cagtattgtt ggttgattta ccctggttat tatataatgt tttccaaaat atatttaaat
     5701 ttgaatcggc aggtaaatat gtttggaagc tcataagttt gtgtgtaata gcttttgttg
     5761 cttcttattt aagttatttt gcttgttcgt atgttaatat cggaaatttc aaagtgataa
     5821 gtttagtgat aaatttagtt atatcggttg tcatatcgat tttagttatt cttctgttta
     5881 atatccgtaa taagaagttt aagtatattt tgaaagttct tacgtcggta ttggtaaaaa
     5941 gatgagaaga gacatcagaa tgatatataa agtaactaca atttttaggc tgccgaatag
     6001 gcgaacgatg tatagttttt gttccaacga agactcttgt tgaaattgga gatgatatga
     6061 aggagttaca attttgactc atgtttatga ttgatcggtt ttaaaagtag tatggagaag
     6121 ctttgggatc atcggagagg atcaaaatag gtaataatgt ttttatagga atgaagtcta
     6181 gaatactaaa gggtgtacat ataggaaata atgtagttat aggtgcaaat agtttagtaa
     6241 acaaggacgt atcagacgat tgtgttgttg caggaaatct agcaaaagta attatgtccc
     6301 tagatgaata ttataaaaag ataaacaata tgaagaatcc gtaaaacttg tgaaactata
     6361 ccgagaataa tatgggaaaa atattgatga aaaagcatta tatgagtttt tagggctgtt
     6421 tacagatgga gatgacaatt tgtcgaagag ttgggagcaa tgcaaaattt agtaggggat
     6481 tataaacaga ctgacaaggt atttaaagaa catgcacaag tatttcataa tattgaagaa
     6541 tttttgaata ctgtgagata ggagttgcta cattgagaaa agtatgtatt tttccaatgt
     6601 aaaagattac gagtttgaaa acgacaccat aatattattt tttaagaaga tattatagag
     6661 cttagg
//
LOCUS       DASJPH010000125         6657 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJPH010000125 DASJPH010000000
VERSION     DASJPH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.127"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U3995_002593"
     CDS             complement(99..1229)
                     /locus_tag="U3995_002593"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1797879.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U3995_002594"
     CDS             complement(1878..3080)
                     /locus_tag="U3995_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1797880.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U3995_002595"
     CDS             complement(3140..3343)
                     /locus_tag="U3995_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1797881.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U3995_002596"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U3995_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U3995_002597"
     CDS             4880..5692
                     /locus_tag="U3995_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797882.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         2022 a         1337 c         1036 g         2262 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tcttttt
//
LOCUS       DASJPH010000126         6644 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJPH010000126 DASJPH010000000
VERSION     DASJPH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.128"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="U3995_002598"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="U3995_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="U3995_002599"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="U3995_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN1797883.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="U3995_002600"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="U3995_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="U3995_002601"
     CDS             complement(2603..3421)
                     /locus_tag="U3995_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1797884.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="U3995_002602"
     CDS             3582..4271
                     /locus_tag="U3995_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1797885.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="U3995_002603"
     CDS             4285..5787
                     /locus_tag="U3995_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1797886.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="U3995_002604"
     CDS             5800..6276
                     /locus_tag="U3995_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1797887.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="U3995_002605"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="U3995_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DASJPH010000127         6593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJPH010000127 DASJPH010000000
VERSION     DASJPH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.129"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..658
                     /locus_tag="U3995_002606"
     CDS             <1..658
                     /locus_tag="U3995_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN1797888.1"
                     /translation="SCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRS
                     IHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            complement(744..1838)
                     /locus_tag="U3995_002607"
     CDS             complement(744..1838)
                     /locus_tag="U3995_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1797889.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2027..2788
                     /locus_tag="U3995_002608"
     CDS             2027..2788
                     /locus_tag="U3995_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1797890.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2903..3343)
                     /gene="fabZ"
                     /locus_tag="U3995_002609"
     CDS             complement(2903..3343)
                     /gene="fabZ"
                     /locus_tag="U3995_002609"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1797891.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3563..4318
                     /gene="fabI"
                     /locus_tag="U3995_002610"
     CDS             3563..4318
                     /gene="fabI"
                     /locus_tag="U3995_002610"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1797892.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4428..5441)
                     /locus_tag="U3995_002611"
     CDS             complement(4428..5441)
                     /locus_tag="U3995_002611"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1797893.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5606..6526
                     /locus_tag="U3995_002612"
     CDS             5606..6526
                     /locus_tag="U3995_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1797894.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2035 a         1307 c         1175 g         2076 t
ORIGIN      
        1 ttcatgcggt atgagaacac tttatcgttt attttctaaa ggaatatttg atattgacac
       61 actaccgatg aaaggtaaaa gaaaacccaa tggccatcag gaaaaacggg gaaaacaaca
      121 gtatcagcgc tcaatccatg atcgacctga taattatcct gatttcaatt ctgagtttgg
      181 tcaccttgaa ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt
      241 tgaaagatta tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat
      301 tgaaactgcc cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac
      361 gtttgactgt ggaaaagaat tttctaactg gaaagccatt agtaaccaac atgatattga
      421 tatatatttt gcggaccctg gaacaccttc tcaacgccca ttaaacgaga attctaacgg
      481 gattctgcgt cgtaatggac tgccgaaatc aatggatttt agaaaagtga atcagacatt
      541 tatttccagt gtcagcaatc aacgtaatca tattccaaga aaatcattga attacagaac
      601 accaatcgaa atatttttga gctatgtaca agaagcattt tattctagct taatttgaca
      661 aatcataacc tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga
      721 ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat
      781 ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca
      841 cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac
      901 ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa
      961 tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc
     1021 cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct
     1081 tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat
     1141 tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt
     1201 aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca
     1261 tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc
     1321 aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag
     1381 tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc
     1441 atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa
     1501 ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg
     1561 taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat
     1621 aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact
     1681 tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa
     1741 acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt
     1801 gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct
     1861 tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc
     1921 tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt
     1981 tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa
     2041 agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa
     2101 caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag
     2161 cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt
     2221 cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt
     2281 atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta
     2341 ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc
     2401 taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa
     2461 caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag
     2521 taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa
     2581 agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga
     2641 tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc
     2701 aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa
     2761 tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca
     2821 acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt
     2881 tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact
     2941 cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca
     3001 ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt
     3061 tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct gattttagga
     3121 taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat
     3181 tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga
     3241 tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag
     3301 gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt
     3361 caggcaattg ccattttcat tactttgact atcaaagtat catattccta aatatttagc
     3421 aatctttttt taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta
     3481 agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat caaattttta
     3541 tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg
     3601 cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc
     3661 agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg
     3721 cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat
     3781 gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc
     3841 caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc
     3901 tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc
     3961 accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta
     4021 taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga
     4081 gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc
     4141 tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga
     4201 tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact
     4261 ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac
     4321 ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc
     4381 ttcctgattt tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact
     4441 tcaatccagc cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac
     4501 aaccgttttg ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg
     4561 tgataaatcc caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca
     4621 aattgatcta gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct
     4681 tcagctaact gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga
     4741 gtgatgaatt ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt
     4801 ttcgtatgtg tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct
     4861 gttcctggaa gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta
     4921 ggagcagcac gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa
     4981 tacgctccta ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca
     5041 atgttttcac cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag
     5101 ggcggcacaa aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt
     5161 tcagggacca ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt
     5221 tcaggtcgaa agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa
     5281 cattgctgac cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct
     5341 gtcaattctc cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt
     5401 ttgatataag aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta
     5461 ttttctccac attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct
     5521 tcattatagt tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga
     5581 atcaaatttt agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga
     5641 ctcttctggc gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt
     5701 ttgggacggg attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct
     5761 gatttttttg atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa
     5821 gtcttttaat cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca
     5881 tagtctttta aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt
     5941 atctgctatc ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc
     6001 aatcggtctt ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat
     6061 ggaacaacag atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac
     6121 cgtcgtatct gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt
     6181 catcccgaat cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact
     6241 tttagatatt cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg
     6301 agtaaatcgt acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt
     6361 tggattagtg gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa
     6421 cggaaaacaa tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc
     6481 ttctggtttc acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat
     6541 gcaacgaatc tcactaaaaa gagcgtggga caaaagtaaa gaacactttt gtc
//
LOCUS       DASJPH010000128         6328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJPH010000128 DASJPH010000000
VERSION     DASJPH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.130"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..1239
                     /locus_tag="U3995_002613"
     CDS             79..1239
                     /locus_tag="U3995_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1797895.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1255..1698
                     /locus_tag="U3995_002614"
     CDS             1255..1698
                     /locus_tag="U3995_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797896.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(2181..2711)
                     /locus_tag="U3995_002615"
     CDS             complement(2181..2711)
                     /locus_tag="U3995_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1797897.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            2816..3367
                     /locus_tag="U3995_002616"
     CDS             2816..3367
                     /locus_tag="U3995_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1797898.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(3520..3993)
                     /locus_tag="U3995_002617"
     CDS             complement(3520..3993)
                     /locus_tag="U3995_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1797899.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            4131..5603
                     /locus_tag="U3995_002618"
     CDS             4131..5603
                     /locus_tag="U3995_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1797900.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            5600..6310
                     /locus_tag="U3995_002619"
     CDS             5600..6310
                     /locus_tag="U3995_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1797901.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         2049 a         1113 c         1231 g         1935 t
ORIGIN      
        1 tcggctttca ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac
       61 ttgcaaggga ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt
      121 cgtttgtttt tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat
      181 gtggattctt taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc
      241 gcagctgaaa ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac
      301 caaggattca atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca
      361 tccagccaat tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat
      421 atttcacttt atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa
      481 ggaacctctt tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt
      541 ttacctaatc gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt
      601 ttgactgttt tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca
      661 gatgacacgt ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt
      721 acgccgtata tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac
      781 gaatcggtaa aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt
      841 attcttgcag gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc
      901 tatcagtata gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca
      961 gggaaaccgg taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga
     1021 aatggtgtgc cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg
     1081 cctggtggaa cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt
     1141 attagaaaag taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag
     1201 cttcaaaaaa atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc
     1261 cgcacaacaa caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat
     1321 gctaacttgg attctcatga attcattcca tcaacttatt gggagaatca agtagaatct
     1381 gttaggaaag ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt
     1441 ggttttgctg gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg
     1501 caacaaagaa tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact
     1561 ttgacagtct atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca
     1621 attcaaacac agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat
     1681 caaaacgctg atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc
     1741 tgggagcatt atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca
     1801 atctttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
     1861 tatgaggaac tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca
     1921 acctcttgat aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa
     1981 aatatgaaaa gctattttct aactagcgtt cttattactc aaagcataac gctgatttca
     2041 caacctcttg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
     2101 aaacatgagg aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc
     2161 acaacctctc tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg
     2221 ctacggatat catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct
     2281 atacctgcat cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa
     2341 gtttcatatt tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca
     2401 gtgatttctg gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt
     2461 tcatttggca taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg
     2521 aatacgccgt ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg
     2581 accattccgc catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat
     2641 tcccaaccat tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta
     2701 aattctttca tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt
     2761 cgattactca tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc
     2821 aagaaaaaat acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg
     2881 atatgaattg aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag
     2941 tcagatttat cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga
     3001 gatcacaggt gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca
     3061 attggcagag tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt
     3121 tctgaaattg tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca
     3181 gcagcgtgac cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt
     3241 tcctactgaa aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat
     3301 caatcgggaa acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa
     3361 aaaataaaaa aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga
     3421 tcaccgttcg gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct
     3481 acttattaaa aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg
     3541 ttcgtagtca gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt
     3601 gtcatgaatg atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt
     3661 atagcgttcg tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg
     3721 gtgcattttc ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc
     3781 atctaaatat tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg
     3841 acaattcgca tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg
     3901 acgtggagac acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt
     3961 ttccaacaaa cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct
     4021 tcccttaatt atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag
     4081 gactaaagta ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca
     4141 accaacccgt agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat
     4201 taattggtac attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat
     4261 tgatgaaagc tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc
     4321 tagtcaacgg gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa
     4381 atttatatct ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac
     4441 ctaattttag tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga
     4501 tgccattatt gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa
     4561 tggggatgtc agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt
     4621 atatcattga taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc
     4681 ttgtcttggt tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca
     4741 ttgatatttt atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt
     4801 ctagtgtagg agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg
     4861 tgattttcat cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac
     4921 ttcgtgtatt caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata
     4981 tggcgatggt aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat
     5041 cagcattcca ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc
     5101 ctgtgacggg tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt
     5161 tcattttgac agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat
     5221 atatcgtgat cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa
     5281 cgacttctgg tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata
     5341 atacattccg tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga
     5401 atgtgacaaa agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata
     5461 aagatcaggc aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca
     5521 tctttgggat tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa
     5581 tgaaagcggg ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc
     5641 tttaacgaac gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact
     5701 aatcttcgtc gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga
     5761 aaaagtaacg gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga
     5821 tatgcttttg tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa
     5881 tgaaaaacaa gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa
     5941 gacagacggc gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat
     6001 gtataaattc tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac
     6061 attcaaaatc ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga
     6121 attagtgaag aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg
     6181 tatggtaaaa gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac
     6241 gaaagcctta gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa
     6301 aggcgaataa tattttgaac accatgta
//
LOCUS       DASJPH010000129         6313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJPH010000129 DASJPH010000000
VERSION     DASJPH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.131"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(119..352)
                     /locus_tag="U3995_002620"
     CDS             complement(119..352)
                     /locus_tag="U3995_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1797902.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(367..1317)
                     /locus_tag="U3995_002621"
     CDS             complement(367..1317)
                     /locus_tag="U3995_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1797903.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1314..2156)
                     /locus_tag="U3995_002622"
     CDS             complement(1314..2156)
                     /locus_tag="U3995_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1797904.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2158..2877)
                     /locus_tag="U3995_002623"
     CDS             complement(2158..2877)
                     /locus_tag="U3995_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1797905.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3569..3715
                     /locus_tag="U3995_002624"
     CDS             3569..3715
                     /locus_tag="U3995_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797906.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            3787..5013
                     /locus_tag="U3995_002625"
     CDS             3787..5013
                     /locus_tag="U3995_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1797907.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(5101..5493)
                     /gene="rpsI"
                     /locus_tag="U3995_002626"
     CDS             complement(5101..5493)
                     /gene="rpsI"
                     /locus_tag="U3995_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1797908.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5509..5952)
                     /gene="rplM"
                     /locus_tag="U3995_002627"
     CDS             complement(5509..5952)
                     /gene="rplM"
                     /locus_tag="U3995_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1797909.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         2135 a         1114 c          925 g         2139 t
ORIGIN      
        1 tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc tattaggata
       61 agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat aaagtctttt
      121 acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc
      181 ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca gatgctggac
      241 taacacttag aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt
      301 cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc atacctaccc
      361 tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata
      421 gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat
      481 aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac tgctttctac
      541 gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg
      601 tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt
      661 cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga
      721 agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag
      781 attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt
      841 tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt ccttcccttt
      901 ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt
      961 gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc ctccagtttc
     1021 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
     1081 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
     1141 atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa
     1201 tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat
     1261 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
     1321 gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
     1381 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata
     1441 aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc
     1501 aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat
     1561 atagttccca cactttgcat agctgttact gctactaaag ttaatagaac catcaataga
     1621 taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg
     1681 attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt
     1741 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa
     1801 tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa
     1861 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
     1921 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
     1981 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     2041 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt
     2101 agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta
     2161 ttccccctct ccaataaata tagtatctcc ataggctttt tttaaatttt cttcattaaa
     2221 tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa
     2281 atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt
     2341 caattttttt aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg
     2401 ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg
     2461 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
     2521 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
     2581 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
     2641 atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt
     2701 aatggagacc tctccctgat gagggataat atttaacatt gctttaagca atgtggattt
     2761 tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat
     2821 ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt ctaacataac
     2881 tttctccttc taaatgataa tgattatcac gatcatatgg tatacaaaaa ttatatttta
     2941 gtcaacccta aattttatag tttagatttt tttctgttgc tgaagattag aatgacaaaa
     3001 ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata tataaaaaag gagcgttctc
     3061 aaatgagaac atgctcctaa aactacttta ttggcgtatg gaaaaattat acttgaagtg
     3121 gatagcgggt tgaataagtt gataagcatc aaaacattta taaaaattat tcaagactat
     3181 gtctgttcca tatacaaata tgaaaatcag taaactccat ttaacttttt tcgtagtgtg
     3241 tctttttata catgtttatc gttattaata gtgaatagga aaaaaactat ttttgatcat
     3301 tgacaattaa atacaatttt tgaataacga tccctgctat tcgagctaga aactcaagta
     3361 cgccatgacc tgattagcga caaatactta gagatttctt tttgttctgg gaaaacaaaa
     3421 tgccatgaca atagcagcaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga
     3481 ggtgaaattc ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg
     3541 agatgatttt atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg
     3601 aatcgataat aatactctag ataagtggat tcgtatatat aatttcccag taataagagt
     3661 tggtggtgta gttcgctttg acgtagaaga tattaaagac tttatgaata cttgaaattt
     3721 tcttctcgcc gcatatacta aaattagtga aatatactgc cttacttttt ttaaggagtg
     3781 aggattttgc ctacaaagct ttcaaatggg aaatataaaa ctaatctgag atatccaaaa
     3841 aggtttaaag aaataacagg aattgcatca gaaaagtatc aaaaaacatt tccaaatagg
     3901 caattggcga ttaaagccga aaatgatatg aagaagaaaa tagaaaaagt tttgagagag
     3961 gagaatgcaa actcattaga attaaaagga aaaataacgt ttaaaaaatt ttacgaatct
     4021 aaatggcttc ctagatatga attaggtcaa acaattagat caaacaggcc tcctagtgat
     4081 ataaccatta gtaatacaaa agatatattt cggttgcata ttttacctat gtttggtgaa
     4141 tacgcaatga attatctaaa tatcaatact gagataatat cagatgaatt aaccaaaaaa
     4201 tcgaaagaat atgcaaatat aaaaatcata aaaggatatg ttcgttccat gttcgatatt
     4261 gcagaaatac taaattatat cgaatttaat cgaactacta aaattattca aagtatcact
     4321 gcacctaaaa aaaatgcttt agaagaaaaa agaatacaag aaggtaagca agcactatca
     4381 tcaaaagaac ttactaattg gatagaagca gtaaatgatg attttaataa tcatttactt
     4441 acttttcatg attacacact ttttatgatt accctgtatc ttggtgatag aaaaagtgag
     4501 acttacgctt tgcaatggaa atatattgat tttgaaaaac aaacggtccg tcttaaacat
     4561 actttagata aatatcaaag aaaaaagttt acaaaaggtc gaaaagatac cgtcattcaa
     4621 gtaccagaag ttgtaatgac attgctaagt gaatggaaat cggtacaagc tgatcaattg
     4681 ttgaaattaa aaattaagca aacgttagat cagtatctct ttacttatac taaaccatct
     4741 ggagaagtaa attgtcctgt tcatgccgat tacctaaact atcgaatcaa ttcaattaaa
     4801 agaaggcatc ctgacttagt tcatttatct ccacacaaac tccgtcatac ttatgcaact
     4861 atagctagac aaggtggagc agatatgaat caaatttcaa atgctttaac acactcagat
     4921 atttctacaa caaagatata tgtgaacaca ccagatatag ttgataaggc tgtttttgaa
     4981 gcttttcaga gaggattgaa taagtgtgat tgatatgtgt tcttaaatgt gttcttaaaa
     5041 acaaaagaag cactttccaa ataattgaaa agtgccagaa acgttgatat ataaaggata
     5101 ttaacgtttt gagaattgtg aagctttacg tgctttctta agacctggtt ttttacgttc
     5161 gaccatacgt gcgtcacgag taagtaagcc agcacgttta agagctaaac ggaagtcagg
     5221 atcaacttct agcaatgcac gagcgatacc atgacggata gctcctgatt gtccagcgta
     5281 tcctccgccg tttacgttaa caaatacgtc atatgcgcct tttgtttcag taacagcgaa
     5341 aggttgattg attacttcac gcaagtcagc atgtggaatg tattcttcaa catctttttt
     5401 atttacagta attttgccag ttcctggtac tagacgtacg cgagcaactg cgtttttacg
     5461 acggcctgtg ccgatatatt gaacttgtgc caatgaaatt ccctcctatt agattaagtt
     5521 tgtgatgtct aaaacttctg gttgttgtgc tgcatgtgga tgttctgctc caccgtatac
     5581 gttcaatttt gtaaattgtt tgcggcctaa agtgttttta ggaagcatac cttttacaga
     5641 agtttcgatc aaacgacgag aatttttagc acgcaattcg ccagcagtga ttgatttcaa
     5701 tcctcctggg tagttgctgt gacggtaata aattttgtca gtcgcttttt tacctgttaa
     5761 tttcactttg tctgcattga tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa
     5821 agttggttta tttttaccac gtagtacaga tgcaacaact gttgaaagac gtcccaatgg
     5881 aacatcagtt gcgtctacta cataccattt acgttctact tcgcctggtt tggccatata
     5941 tgttgtacgc acgttatttt cctccaattt aagttctgat gtttgacata cacttcgagt
     6001 ttccggggct ctttgtgggg caaacaatac catttaacat attacccgct aatctagtca
     6061 atgtcaacgt ttttctgctt gattgaaaaa tttatatgaa actttacttc ttttctttct
     6121 tttatataaa agggtcgttt cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt
     6181 cttgtcacaa ccccttattt tgaataaacg gtgttctcct gccaactcct cggtcacaag
     6241 tcacaatatc cataaaacat aagaagctgt tttttaaaat tgtttcttgt gactcggagc
     6301 tgaacggcag gct
//
LOCUS       DASJPH010000130         6195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJPH010000130 DASJPH010000000
VERSION     DASJPH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.132"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..410)
                     /locus_tag="U3995_002628"
     CDS             complement(<1..410)
                     /locus_tag="U3995_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1797910.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQS"
     gene            688..1449
                     /locus_tag="U3995_002629"
     CDS             688..1449
                     /locus_tag="U3995_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1797911.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            1475..4066
                     /gene="mprF"
                     /locus_tag="U3995_002630"
     CDS             1475..4066
                     /gene="mprF"
                     /locus_tag="U3995_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1797912.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(4254..5441)
                     /gene="tuf"
                     /locus_tag="U3995_002631"
     CDS             complement(4254..5441)
                     /gene="tuf"
                     /locus_tag="U3995_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1797913.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            5809..6165
                     /locus_tag="U3995_002632"
     CDS             5809..6165
                     /locus_tag="U3995_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1797914.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         1785 a         1182 c         1162 g         2066 t
ORIGIN      
        1 gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc
       61 acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca
      121 taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct
      181 tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc aatactgtca
      241 gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct
      301 gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc
      361 atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc
      421 gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg
      481 taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt
      541 gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg
      601 tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg
      661 ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta
      721 ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac
      781 tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc
      841 cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat
      901 agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc
      961 gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt
     1021 agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga
     1081 ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc
     1141 agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa
     1201 aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa
     1261 aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat
     1321 tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga
     1381 catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct
     1441 gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct
     1501 gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt
     1561 cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc
     1621 cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc
     1681 tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta
     1741 cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt
     1801 tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat
     1861 ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct
     1921 ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg
     1981 gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa
     2041 aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt
     2101 ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat
     2161 tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat
     2221 gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg
     2281 tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat
     2341 tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg
     2401 ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt
     2461 attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga
     2521 gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag
     2581 tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg
     2641 agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga
     2701 ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa
     2761 aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat
     2821 cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt
     2881 ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt
     2941 ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct
     3001 tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct
     3061 tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt
     3121 tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa
     3181 atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc
     3241 ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga
     3301 gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta
     3361 tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt
     3421 gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga
     3481 gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg
     3541 gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa
     3601 aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa
     3661 agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga
     3721 tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt
     3781 agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat
     3841 tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat taagagaata
     3901 taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg
     3961 gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca
     4021 aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga
     4081 tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct
     4141 acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga
     4201 ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct
     4261 tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc
     4321 gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca
     4381 ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg
     4441 aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt
     4501 gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca
     4561 agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta
     4621 tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct
     4681 ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca
     4741 cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact
     4801 ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta
     4861 tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg
     4921 gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga
     4981 acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag
     5041 aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga
     5101 ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct
     5161 gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt
     5221 ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct
     5281 ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact
     5341 gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg
     5401 ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa
     5461 taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta
     5521 aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt
     5581 caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt
     5641 ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt
     5701 gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa
     5761 tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta
     5821 attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat
     5881 ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc
     5941 caagcagata acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa
     6001 gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat
     6061 gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag
     6121 gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa
     6181 ttttattaga taaaa
//
LOCUS       DASJPH010000131         6173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJPH010000131 DASJPH010000000
VERSION     DASJPH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.133"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U3995_002633"
     CDS             725..811
                     /locus_tag="U3995_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1797915.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U3995_002634"
     CDS             complement(932..1621)
                     /locus_tag="U3995_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797916.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U3995_002635"
     CDS             complement(1628..1906)
                     /locus_tag="U3995_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797917.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U3995_002636"
     CDS             complement(1866..2780)
                     /locus_tag="U3995_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1797918.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U3995_002637"
     CDS             complement(2762..3142)
                     /locus_tag="U3995_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1797919.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U3995_002638"
     CDS             complement(3592..3879)
                     /locus_tag="U3995_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1797920.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U3995_002639"
     CDS             complement(3899..4123)
                     /locus_tag="U3995_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1797921.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U3995_002640"
     CDS             complement(4578..4748)
                     /locus_tag="U3995_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797922.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U3995_002641"
     CDS             complement(4763..5296)
                     /locus_tag="U3995_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1797923.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U3995_002642"
     CDS             complement(5317..6159)
                     /locus_tag="U3995_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1797924.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASJPH010000132         5896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJPH010000132 DASJPH010000000
VERSION     DASJPH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.134"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1292
                     /locus_tag="U3995_002643"
     CDS             138..1292
                     /locus_tag="U3995_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1797925.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1286..1708
                     /locus_tag="U3995_002644"
     CDS             1286..1708
                     /locus_tag="U3995_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797926.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2842..3810
                     /locus_tag="U3995_002645"
     CDS             2842..3810
                     /locus_tag="U3995_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1797927.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3820..4308
                     /locus_tag="U3995_002646"
     CDS             3820..4308
                     /locus_tag="U3995_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1797928.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4604..4885
                     /locus_tag="U3995_002647"
     CDS             4604..4885
                     /locus_tag="U3995_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797929.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4887..5303
                     /locus_tag="U3995_002648"
     CDS             4887..5303
                     /locus_tag="U3995_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797930.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2187 a         1016 c          966 g         1727 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac ctctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     2761 ggggtcttta attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag
     2821 ttaatcttga aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat
     2881 acgattcctt tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct
     2941 agaatgcgtg atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta
     3001 agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac
     3061 aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt
     3121 gttttattta atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa
     3181 gtccatgaga aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc
     3241 caacaattca atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa
     3301 tacagaacca aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc
     3361 cctaaaagtt atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa
     3421 ctaaccgcaa tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag
     3481 cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac
     3541 aaacatgctc caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt
     3601 caatctgaaa aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta
     3661 gaacttttac aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt
     3721 aacaaacaaa tagcagataa caattccgat attaccccag aatttaaaga aaacttacta
     3781 gataatctcc aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa
     3841 gaattagcag atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta
     3901 cctagtaatt ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca
     3961 acaattacaa aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat
     4021 tcattaccta ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa
     4081 aaagataggc aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac
     4141 cagcaacaac aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc
     4201 cagctaacct atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact
     4261 caaaaaggac aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt
     4321 tattcataaa aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat
     4381 ctcacgtcgc tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt
     4441 gctaggtagg ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca
     4501 ttgtggatag cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt
     4561 atgatataca taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg
     4621 gaatattgat ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa
     4681 aactggcaaa aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga
     4741 ccaggcactt aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga
     4801 tatacaagca aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat
     4861 tgaaacactt atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc
     4921 tttatcccag tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata
     4981 actactcgga agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa
     5041 ctaccagcaa ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa
     5101 tcattgcaga atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt
     5161 ctaaagtata tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa
     5221 atcaacccaa aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc
     5281 caacaaactt tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct
     5341 tttttagggg gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag
     5401 aaaaatccgc ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa
     5461 tatcggttat ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa
     5521 gactgctcta acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc
     5581 gcttatgaag cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa
     5641 ggaatcggtt aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg
     5701 aacgtacctt ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata
     5761 caagggctac ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt
     5821 cccaaaacaa tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa
     5881 ttataggtgt atatca
//
LOCUS       DASJPH010000133         5769 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJPH010000133 DASJPH010000000
VERSION     DASJPH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.135"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1014..1304)
                     /locus_tag="U3995_002649"
     CDS             complement(1014..1304)
                     /locus_tag="U3995_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797931.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="U3995_002650"
     CDS             1706..1942
                     /locus_tag="U3995_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797932.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="U3995_002651"
     CDS             1949..3901
                     /locus_tag="U3995_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1797933.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U3995_002652"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U3995_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1797934.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="U3995_002653"
     CDS             complement(4797..5489)
                     /locus_tag="U3995_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1797935.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DASJPH010000134         5689 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJPH010000134 DASJPH010000000
VERSION     DASJPH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.136"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..2263
                     /gene="acm"
                     /locus_tag="U3995_002654"
     CDS             98..2263
                     /gene="acm"
                     /locus_tag="U3995_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1797936.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2535..3020)
                     /locus_tag="U3995_002655"
     CDS             complement(2535..3020)
                     /locus_tag="U3995_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1797937.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3110..3455
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3500..4141
                     /locus_tag="U3995_002656"
     CDS             3500..4141
                     /locus_tag="U3995_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1797938.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4160..4795
                     /locus_tag="U3995_002657"
     CDS             4160..4795
                     /locus_tag="U3995_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1797939.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4813..5628
                     /locus_tag="U3995_002658"
     CDS             4813..5628
                     /locus_tag="U3995_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1797940.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2019 a          898 c         1172 g         1600 t
ORIGIN      
        1 aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga tgatatagta
       61 ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat
      121 tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc
      181 ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat
      241 tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca
      301 accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt
      361 ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa
      421 agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg
      481 ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc
      541 attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg
      601 ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca
      661 cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat
      721 cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat
      781 acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga
      841 tttccccagc gctacattta atttttcagt aactgataat tacattgaaa taactatacc
      901 aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc
      961 agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc
     1021 tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa
     1081 cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat
     1141 cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag
     1201 tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga
     1261 ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa
     1321 tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa
     1381 caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag
     1441 accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga
     1501 tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca
     1561 gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa
     1621 tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa
     1681 taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg
     1741 caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa
     1801 agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga
     1861 cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat
     1921 tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg
     1981 tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa
     2041 tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag
     2101 caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct
     2161 tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac
     2221 tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt
     2281 ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc
     2341 atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga
     2401 agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa
     2461 agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga
     2521 aaactactct atcattacaa attttcataa actggcagat gttctaagta aacaagtatc
     2581 ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct
     2641 acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg
     2701 tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt
     2761 tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg
     2821 gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat
     2881 aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc
     2941 gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct
     3001 tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa
     3061 agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc
     3121 gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc
     3181 agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg
     3241 ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag
     3301 gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga
     3361 ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata
     3421 aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat
     3481 tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg
     3541 agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac cattagggat
     3601 tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata
     3661 tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt
     3721 gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa
     3781 ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca
     3841 atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat
     3901 cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa
     3961 agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc
     4021 gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt
     4081 gactggtatt ctaacaatca taatgaaaca agttgaagga aaattaaatt attaccgcta
     4141 actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa
     4201 aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg
     4261 tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga
     4321 cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga
     4381 acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga
     4441 aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa
     4501 agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca
     4561 gctttctggc ggacaaaaac agcgggtagc tattgccaga gcattggcta tgaatccacg
     4621 ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga
     4681 gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt
     4741 agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag
     4801 gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt
     4861 tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag
     4921 aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag agataaagac
     4981 gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt
     5041 acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg
     5101 atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt
     5161 acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca
     5221 aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat
     5281 gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg
     5341 tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg
     5401 atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac
     5461 ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt
     5521 acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa
     5581 aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac
     5641 tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttct
//
LOCUS       DASJPH010000135         5585 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJPH010000135 DASJPH010000000
VERSION     DASJPH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.137"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="U3995_002659"
     CDS             complement(488..1297)
                     /locus_tag="U3995_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797941.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="U3995_002660"
     CDS             complement(1297..2043)
                     /locus_tag="U3995_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1797942.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="U3995_002661"
     CDS             complement(2061..2528)
                     /locus_tag="U3995_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797943.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="U3995_002662"
     CDS             complement(2528..2938)
                     /locus_tag="U3995_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1797944.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="U3995_002663"
     CDS             complement(2949..3962)
                     /locus_tag="U3995_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1797945.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="U3995_002664"
     CDS             4179..4913
                     /locus_tag="U3995_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797946.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="U3995_002665"
     CDS             4897..>5585
                     /locus_tag="U3995_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1797947.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DASJPH010000136         5569 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJPH010000136 DASJPH010000000
VERSION     DASJPH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.138"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..862
                     /locus_tag="U3995_002666"
     CDS             5..862
                     /locus_tag="U3995_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1797948.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="U3995_002667"
     CDS             884..1393
                     /locus_tag="U3995_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1797949.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="U3995_002668"
     CDS             1430..1831
                     /locus_tag="U3995_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797950.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="U3995_002669"
     CDS             1943..2296
                     /locus_tag="U3995_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797951.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="U3995_002670"
     CDS             2330..2773
                     /locus_tag="U3995_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1797952.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="U3995_002671"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="U3995_002671"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1797953.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="U3995_002672"
     CDS             3868..4581
                     /locus_tag="U3995_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1797954.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4788..4937)
                     /locus_tag="U3995_002673"
     CDS             complement(4788..4937)
                     /locus_tag="U3995_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797955.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5333..5533
                     /locus_tag="U3995_002674"
     CDS             5333..5533
                     /locus_tag="U3995_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1797956.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1934 a          854 c         1210 g         1571 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     4681 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
     4741 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
     4801 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
     4861 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
     4921 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
     4981 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
     5041 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
     5101 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
     5161 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
     5221 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
     5281 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
     5341 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
     5401 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
     5461 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
     5521 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaa
//
LOCUS       DASJPH010000137         5554 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJPH010000137 DASJPH010000000
VERSION     DASJPH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.139"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..2188
                     /locus_tag="U3995_002675"
     CDS             128..2188
                     /locus_tag="U3995_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1797957.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2271..2840
                     /locus_tag="U3995_002676"
     CDS             2271..2840
                     /locus_tag="U3995_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1797958.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3015..3821)
                     /locus_tag="U3995_002677"
     CDS             complement(3015..3821)
                     /locus_tag="U3995_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797959.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3959..5473
                     /locus_tag="U3995_002678"
     CDS             3959..5473
                     /locus_tag="U3995_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1797960.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1977 a          964 c         1120 g         1493 t
ORIGIN      
        1 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
       61 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
      121 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
      181 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
      241 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
      301 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
      361 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
      421 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
      481 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
      541 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
      601 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
      661 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
      721 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
      781 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
      841 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
      901 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
      961 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     1021 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     1081 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     1141 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     1201 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     1261 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     1321 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     1381 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     1441 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     1501 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     1561 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     1621 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     1681 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     1741 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     1801 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     1861 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     1921 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     1981 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     2041 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     2101 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     2161 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     2221 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     2281 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     2341 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     2401 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     2461 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     2521 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     2581 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     2641 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     2701 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     2761 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     2821 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     2881 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     2941 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     3001 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     3061 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     3121 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     3181 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     3241 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     3301 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     3361 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     3421 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     3481 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     3541 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     3601 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     3661 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     3721 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     3781 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     3841 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     3901 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     3961 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     4021 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     4081 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     4141 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     4201 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     4261 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     4321 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     4381 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     4441 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     4501 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     4561 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     4621 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     4681 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     4741 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     4801 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     4861 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     4921 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     4981 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     5041 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     5101 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
     5161 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
     5221 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
     5281 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
     5341 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
     5401 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
     5461 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
     5521 ataccgaggg tcagctgatt gaacactgtt tagt
//
LOCUS       DASJPH010000138         5481 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJPH010000138 DASJPH010000000
VERSION     DASJPH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.140"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U3995_002679"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U3995_002679"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="U3995_002680"
     CDS             complement(580..1353)
                     /locus_tag="U3995_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1797961.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="U3995_002681"
     CDS             1497..1847
                     /locus_tag="U3995_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1797962.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="U3995_002682"
     CDS             1828..2544
                     /locus_tag="U3995_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1797963.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="U3995_002683"
     CDS             2544..3992
                     /locus_tag="U3995_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1797964.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="U3995_002684"
     CDS             complement(4062..4571)
                     /locus_tag="U3995_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797965.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="U3995_002685"
     CDS             complement(4561..5286)
                     /locus_tag="U3995_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797966.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DASJPH010000139         5339 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJPH010000139 DASJPH010000000
VERSION     DASJPH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.141"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..278)
                     /locus_tag="U3995_002686"
                     /pseudo
     CDS             complement(111..278)
                     /locus_tag="U3995_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="U3995_002687"
     CDS             complement(544..738)
                     /locus_tag="U3995_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797967.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="U3995_002688"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="U3995_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="U3995_002689"
     CDS             2090..3130
                     /locus_tag="U3995_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1797968.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /locus_tag="U3995_002690"
     CDS             3954..4286
                     /locus_tag="U3995_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1797969.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="U3995_002691"
     CDS             4905..5246
                     /locus_tag="U3995_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1797970.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          727 c          900 g         1769 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata
     4861 taaagcatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DASJPH010000140         5311 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJPH010000140 DASJPH010000000
VERSION     DASJPH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.142"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U3995_002692"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U3995_002692"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1797971.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U3995_002693"
     CDS             complement(1297..2133)
                     /locus_tag="U3995_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1797972.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U3995_002694"
     CDS             complement(2317..3534)
                     /locus_tag="U3995_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1797973.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U3995_002695"
     CDS             complement(3683..3862)
                     /locus_tag="U3995_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797974.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U3995_002696"
     tRNA            3917..4000
                     /locus_tag="U3995_002696"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="U3995_002697"
     CDS             complement(4240..4818)
                     /locus_tag="U3995_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797975.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="U3995_002698"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="U3995_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DASJPH010000141         5189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJPH010000141 DASJPH010000000
VERSION     DASJPH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.143"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="U3995_002699"
     CDS             137..748
                     /locus_tag="U3995_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1797976.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="U3995_002700"
     CDS             821..1750
                     /locus_tag="U3995_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1797977.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="U3995_002701"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="U3995_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="U3995_002702"
     CDS             complement(2693..3871)
                     /locus_tag="U3995_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1797978.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="U3995_002703"
     CDS             3969..4067
                     /locus_tag="U3995_002703"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1797979.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="U3995_002704"
     CDS             4098..4499
                     /locus_tag="U3995_002704"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1797980.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="U3995_002705"
     CDS             complement(4706..>5189)
                     /locus_tag="U3995_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1797981.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DASJPH010000142         5152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJPH010000142 DASJPH010000000
VERSION     DASJPH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.144"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..561)
                     /locus_tag="U3995_002706"
     CDS             complement(52..561)
                     /locus_tag="U3995_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1797982.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="U3995_002707"
     CDS             complement(673..1671)
                     /locus_tag="U3995_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1797983.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1872..2456
                     /locus_tag="U3995_002708"
     CDS             1872..2456
                     /locus_tag="U3995_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1797984.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2533..3726)
                     /locus_tag="U3995_002709"
     CDS             complement(2533..3726)
                     /locus_tag="U3995_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1797985.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            3790..4170
                     /locus_tag="U3995_002710"
     CDS             3790..4170
                     /locus_tag="U3995_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1797986.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4244..4606
                     /locus_tag="U3995_002711"
     CDS             4244..4606
                     /locus_tag="U3995_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797987.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1684 a          891 c          958 g         1619 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaatattag
     1861 gaggaaacaa tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg
     1921 ttatcaaaac aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga
     1981 cggttcaaaa tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc
     2041 gcgttattcc tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg
     2101 aaagtattta tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg
     2161 gagctttatc tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa
     2221 ataacacaaa tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag
     2281 aaatcattga agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct
     2341 ttggacatgt actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc
     2401 cacaagaccg acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg
     2461 gtatggtacg gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt
     2521 tgaataaatg atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag
     2581 taaagtttct tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa
     2641 cgtgatgcca ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg
     2701 gtcgtctgaa atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat
     2761 tattctcact ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc
     2821 tagataagga atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc
     2881 aatcagtcca aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc
     2941 agctttcatc tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa
     3001 agtcttggtt gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag
     3061 catggaagta aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag
     3121 aatcacttga tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac
     3181 acgccctcca ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat
     3241 ttttttctcc atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct
     3301 tcgaacgatg tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc
     3361 atgaatcaaa atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt
     3421 taacacacca cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg
     3481 ttcccaatga atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc
     3541 atgtttgatg tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc
     3601 tgctacccac aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt
     3661 tacactttct gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc
     3721 agtcattttc gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga
     3781 gggaagtaga tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag
     3841 ccttatggcg cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa
     3901 gtacaatccg gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca
     3961 gttcagatca tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta
     4021 gaaaaaacaa atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt
     4081 aagatcggat ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct
     4141 gaactagaac ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga
     4201 atttttatgt aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt
     4261 tgctggtaca attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat
     4321 tgatgacaag ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa
     4381 tatacttaat ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga
     4441 attgacaaat acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca
     4501 aaagaatcaa agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg
     4561 ctctgttctt ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc
     4621 gaacaaagaa gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac
     4681 aatgaaatgt tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa
     4741 acggttgtat aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa
     4801 tttatagttg accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga
     4861 tgttatatca catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg
     4921 ataagataac tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa
     4981 cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag
     5041 atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc
     5101 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tc
//
LOCUS       DASJPH010000143         5138 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJPH010000143 DASJPH010000000
VERSION     DASJPH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.145"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="U3995_002712"
     CDS             complement(<1..448)
                     /locus_tag="U3995_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1797988.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="U3995_002713"
     CDS             complement(755..1852)
                     /locus_tag="U3995_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1797989.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="U3995_002714"
     CDS             complement(1960..3987)
                     /locus_tag="U3995_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1797990.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4863)
                     /locus_tag="U3995_002715"
     CDS             complement(4132..4863)
                     /locus_tag="U3995_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1797991.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1829 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatt
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagcgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga cttgacgaga gtgtaaaa
//
LOCUS       DASJPH010000144         5052 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJPH010000144 DASJPH010000000
VERSION     DASJPH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.146"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..638)
                     /locus_tag="U3995_002716"
     CDS             complement(<1..638)
                     /locus_tag="U3995_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1797992.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAAN"
     gene            complement(648..2024)
                     /locus_tag="U3995_002717"
     CDS             complement(648..2024)
                     /locus_tag="U3995_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1797993.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2144..2791)
                     /locus_tag="U3995_002718"
     CDS             complement(2144..2791)
                     /locus_tag="U3995_002718"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN1797994.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2875..4302)
                     /gene="uxaC"
                     /locus_tag="U3995_002719"
     CDS             complement(2875..4302)
                     /gene="uxaC"
                     /locus_tag="U3995_002719"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1797995.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4376..>5052)
                     /gene="uxuA"
                     /locus_tag="U3995_002720"
     CDS             complement(4376..>5052)
                     /gene="uxuA"
                     /locus_tag="U3995_002720"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1797996.1"
                     /translation="PTDPEEIIREVENSTNNFELPGWEPHRLSQIKQLLEAYKEVDEE
                     KLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLPRIVKNRDDLDWLCNVVDSP
                     SNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRNIKYLSDNGLDFYEAPHKSE
                     YGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEAL
                     EKGASK"
BASE COUNT         1646 a         1033 c          656 g         1717 t
ORIGIN      
        1 ttatttgcag cttctagatc accaggagaa tcaccaatca ttaatatctt atccaaatca
       61 caaccatttt caattacact tcgaataata tcttcttttt ttcctttatc ttgacaacac
      121 agttcatcaa ctaatgataa taattcatgt ctttcccatt cttcttgaac agcttcctta
      181 ttagctgagc ttacaataat tatcttccca tattttttca gagcttttat agtatctcta
      241 gcacttaaaa agggtttatc taatcctttt gcttgactaa tctgttgatt gacttcttcc
      301 gaccatttta aagcttttaa taaatcctct gactgagaat attctatttc cctcttcaaa
      361 gatggattag acaattcttt tgttagagat acccagtgct ttaatcgatt aatcgatgtt
      421 acatcatttt tttctaatgt tttaactaaa cctaaaaatc tgtttattcc acgagtctct
      481 gaatataaat taatattatt ccaatcctgt aggaattttt ctctatgatg aacatcaaaa
      541 atctctgcag caattggtcc gaaaaagaga ttgtgtttat aagtcatcgt atccataaca
      601 cagccatctg aatcaataca aaatatccaa ttattcatat ttctctccta tttatgtttt
      661 tgttttaaaa gctcgcaatt ttctaagact ttatctttag ataatggata aaggaaaaat
      721 aaaattaatc ctacaatcag aaaacaaatt gatggaatta atgttgaagc gttatataac
      781 ttttctaaaa catctcccgt ttgtacattt gctaaagagt catagcctat aatagaaaga
      841 gtccaacctc cgacgcctcc agctagagct tgacctattt ttctagcaaa agaatatacc
      901 gcatatacgg tcccatcttc tctttgatca gactttactt cttgatcatc aataacatca
      961 gtaatatttg cccaaataac agtattaaac acacccaaac ccatatagcc aaccgaacta
     1021 agaattaaga atacatacat attactagct tttatcaaaa ataagatcaa atatactatt
     1081 gctgaaaaga acattcctac tgatgccagt tctttttttc catatttttt acttaaaaac
     1141 aaagttaaag gcgaaacaac tattaaaacc aaaataggat tgataaaatt cattaaaact
     1201 attccctgtg catcattata gtaatcagga aatacataat tattcatact cataattaat
     1261 aattgagcaa ggagtaagaa aatagccgca ccaattatag aaagtaatgc cctattttta
     1321 actaaccctt ttaaagattt taacaatgaa actttgtatt gagattggtc aattcttact
     1381 cgttcagtag ttagtttata acatattaaa taacaaataa gtgcacatat cgaaaatatt
     1441 cccgcaacaa ttgtaaaagt tgatccacca cgaactattt gagcgcctga atcatctctt
     1501 gtataaataa ataatggtgt taatacacca atgaccaaac ttgctaaagt agctccaaca
     1561 tttcggaatg tcgatagcat tgttctttct tctgtaatag aagtaatagc tgatgccatt
     1621 gatccataag gaatatttat agatgtgtaa aaaatactcc cccataatat atacgtaaca
     1681 tacatgtata ctaccttaac gctcatagaa gctgaaatca tagaagtttg atacattaaa
     1741 aaacttgcta aagcgactgg accactcatt cttaaaatcc acggtttaaa cttaccctgc
     1801 ttagttgctt tcgatctatc aacaatacgc cccatagtta catcagtaac tgcatcgata
     1861 aatcttgcta ctaaaaataa tgttccaacg gcagctccgc ttatccccaa tacttttgta
     1921 taaaagatca ttaaaaaaga actagcaaaa atgaaagtaa aatcgtttcc aaaatctcca
     1981 aacatatatc ctattttgtc tcttatacca aattttcttt ccatacaatt ctctcccttc
     2041 tgttgttaag cgttttcatt ctgatgaaag tacgtactat actctgaagc gtaaagagtc
     2101 agtatttatt ttttacattt cagagtatta tttgtttttt ctcctaataa ttctttatct
     2161 cataaaactt atctatatat ttactagcta ctcgacttac ttcttcaaaa tcaccttttt
     2221 caacagaagc caataaattc ccacctacac caactgcaac aactcctact tcaaaccaat
     2281 cttctaagtt ctctatactt actccgccag taggcattat atttacgtgt ggtaatggag
     2341 cttgataggc ttttacaatg gatggaccaa aagcagaacc tgggaatagt ttaatgatat
     2401 ctacgccagc agtaagtgcc tcctttatct cagtaattgt catacaacct ggtaaataag
     2461 gaatttgata taaattacaa atctcagctg tttgtcgatc aaaagatgga ctaacgatat
     2521 attctgcccc agccataatt gcgatacgag cagtaaacgc atctaaaacc gtaccggctc
     2581 cgattattac atctttttga tcagcatatt ttttagcaat ttctttgatt atttcttcag
     2641 cattaggaac tgtaaaagtt agttcaatcc ctttaattcc acccgcaata attgaattta
     2701 ttgctttaat ggcctcatcc ttatttttcc cacgtacaac cgcaatgact ccagccgctt
     2761 ctaacttact taaaatttct acttttttca tatctatctc cttcctttat ggatacatac
     2821 tttgtctagc tgtactaact tacaaagaat aaatttaagc ttttatttca agtattattt
     2881 tctacttccg atttcaaaaa aattaaaata attatacgca ttacgataac aaatattctc
     2941 cactatctga ctaagataca tttcatcatc tggaattcgg cctgctttaa cccactcgct
     3001 tagtaattga cataatactc gacgaaaata ctcatggcga ggatagctta gaaaacttcg
     3061 agaatcagtc aacatacctg taaaattcga caacaaactt tgttgtgcca tcgtagtcaa
     3121 ctgttgacgc attccttcag ctgtatcatt gaaccaccaa gcacatccca attggatttt
     3181 ttgaatcata cccccttgga agctttgcat tgctgttgct agtggaagcc aatcagttgg
     3241 atttaatgaa tataaaattg tttttggtaa gcacccactc ttttcagcgg tatcgaacaa
     3301 tgctacaaca ttttgtgtca aactagcttg atcccccatc gaatcaccac cgacatctac
     3361 tccaatttgt tcctttaata tacttgaatt atttcttaaa gcatttaaat gcatttgcat
     3421 cgtccaacca tatcttgtat actcagacat caaatgtaat tgaatacctg taacaaattg
     3481 tgcaatttct gataaatgga aatccgtttt tcctgctaaa gcttttatta acagattgtc
     3541 gagtattttt tcatctgcta tttcataaaa ataagtattc aaaccgtgat ctgctaatcg
     3601 acagccaatt tgatgaaaat gatccactcg ttggctcaat gcttgcttca acccattaaa
     3661 gtctttgatt ggtatagccg ctgtttgact aagttttaaa atatagttcg aaaagcctga
     3721 tgatttgata ctccagacct tatctggacg gaaagtaggt aataccttga aagaattatc
     3781 ctgtttagaa agctcagtat gataccacaa gtcatctgta ggatcatccg ttgtacagat
     3841 ggctttcaca tccatcatct ccaacaattc tttcgggcga taggaatccg tttgaatttt
     3901 ttcgttagca cgtcgccaaa tttctggtgc attcttctca gaaattgtca aatcaatatc
     3961 aaagtaacgt tgtaattcca aatgagacca ttcaaaaata ggattacctg gcgccttctc
     4021 cactgttttt acaaaagcta aaaatttttc ataatcatca cctggaccac tgatcaactc
     4081 ttcttctata ccattagctc gcatcaatcg ccatttataa tgatctccag ctcctttatc
     4141 atttaaccat acctgagtca aattcttgta ttgcttattt tctagcaaat atgctgggtc
     4201 taaatgacaa tgatagtcga taattggttg gtgttttgca aatttatgaa aaagtctttt
     4261 agctatttca ttctctaata aaaagttctt atctaaaaac atatttaaac actcctttcc
     4321 ttccgttagt ttttagaaga gaaaattttt cctctaaata aactccctta tataattatt
     4381 tactcgcccc cttttcaagc gcttcccaaa taccatttaa ataaactgca cctagagctc
     4441 ggtcatataa tccataacca ggtctaccgg tttctcccca aatcatccga ccatgatctg
     4501 gacgaatata gccattaaat ccattatcgt acattgtttt taatatctta tacatatcta
     4561 gagaaccata ttctgattta tgcggcgctt cgtaaaaatc gagtccattg tcacttaaat
     4621 atttgatatt acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa
     4681 tttcatagac atcattatct ggtcgttctg caatacttcc agtacataat gtaataccat
     4741 tagaaggaga atccactaca ttacacaacc aatctaaatc gtcacgattt ttaacaatac
     4801 gaggtaatcc gaaaatggaa tatggtggat catctgggtg aactgccatt ttaatcccaa
     4861 cttcttcaca tgtcgggata attgctttga gaaaatatgc aaagttctct cgtaatttct
     4921 cttcatcaac ttctttatag gcttctaata attgcttaat ttgtgataac cgatgaggtt
     4981 cccaacccgg caattcaaaa ttattagtag aattttctac ttctctaata atttcctccg
     5041 gatcagtagg ga
//
LOCUS       DASJPH010000145         4972 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJPH010000145 DASJPH010000000
VERSION     DASJPH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.147"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U3995_002721"
     CDS             complement(<1..182)
                     /locus_tag="U3995_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1797997.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U3995_002722"
     CDS             517..951
                     /locus_tag="U3995_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1797998.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U3995_002723"
     CDS             974..1294
                     /locus_tag="U3995_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1797999.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U3995_002724"
     CDS             1435..1626
                     /locus_tag="U3995_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798000.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="U3995_002725"
     CDS             complement(1697..1954)
                     /locus_tag="U3995_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1798001.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="U3995_002726"
     CDS             2112..2375
                     /locus_tag="U3995_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798002.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="U3995_002727"
     CDS             2399..2587
                     /locus_tag="U3995_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798003.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="U3995_002728"
     CDS             complement(2670..2855)
                     /locus_tag="U3995_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798004.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="U3995_002729"
     CDS             3070..3441
                     /locus_tag="U3995_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1798005.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="U3995_002730"
     tRNA            complement(3591..3663)
                     /locus_tag="U3995_002730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="U3995_002731"
     CDS             complement(3709..4137)
                     /locus_tag="U3995_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798006.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="U3995_002732"
     CDS             complement(4197..4955)
                     /locus_tag="U3995_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1798007.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1735 a          780 c          975 g         1482 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tc
//
LOCUS       DASJPH010000146         4946 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJPH010000146 DASJPH010000000
VERSION     DASJPH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.148"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..1770
                     /locus_tag="U3995_002733"
     CDS             127..1770
                     /locus_tag="U3995_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1798008.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1843..2388)
                     /locus_tag="U3995_002734"
     CDS             complement(1843..2388)
                     /locus_tag="U3995_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1798009.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2411..2896)
                     /locus_tag="U3995_002735"
     CDS             complement(2411..2896)
                     /locus_tag="U3995_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1798010.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2886..3698)
                     /locus_tag="U3995_002736"
     CDS             complement(2886..3698)
                     /locus_tag="U3995_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1798011.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3837..>4946)
                     /locus_tag="U3995_002737"
     CDS             complement(3837..>4946)
                     /locus_tag="U3995_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1798012.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1592 a          977 c          946 g         1431 t
ORIGIN      
        1 aaataaagct caacgaacgc acttagcaga ttcgttgagc ttttcgtttc tagttaccgg
       61 taacaaggat acttctaaaa tagaatcggt tataatcaag agggaccgtt aaaggagtga
      121 aacgatatga tacaagaaca aaaatggtgg aaagaagcag taggctatca aatctatcct
      181 gctagtttca aagatagcaa tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa
      241 cttaattatt tgaaagatct tggtatcggc tttatctgga tcaatccgat ctatcaatct
      301 ccatttgtcg ataatggtta tgacgtaagt gactatcaag atatagaaga gagattcgga
      361 acgatggaag attttgattt attgctaaaa gaagcacatg aattagggat caaagtgatt
      421 atggatcttg tcatcaacca tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa
      481 tctaaagaaa gcccgtatcg cgattattac atttgggtcg acggggtaga tggcaaagaa
      541 ccgaataatt ggacatcgat tttcggcggt tctgcttggg agtattctca tgaaactgga
      601 cagtattatc tgcatgtttt tgcaaaagaa caaccagact taaattggga aagcgagaaa
      661 ttgaaagaag aactattcaa tatgatccga tggtggttgg ataaagggat agatggattt
      721 cgtttagatg cgatttctca tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac
      781 cctttctcac cctttcaaaa tgtctcagga atcgaagaac atttgaccga tttgaaacat
      841 gtttttgaag agtacgatat catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa
      901 ggaccacaat gggtagggaa agacggttat ttcgatatga tattcgaatt tgatcatatc
      961 catatctggc agcaagaaaa agaagggcaa ttagatgtcc ttaaattgaa acttgcatta
     1021 agcgcttggc aaacttcatt ggatggtatc ggatggaacg ctttatatat ggaaaaccat
     1081 gatgttcctc gtgccgtttc agtgtttgga gatactcgtc ccgatttttg ggctatgtct
     1141 gcaaaagcaa tcgctatgat gtatttcttt ttgcaaggta cgccatttat ttatcaagga
     1201 caggaaatcg gaatgacgaa tatgcccttt gaatcgatcg atcaagttga tgcagtagat
     1261 tcgaaaagac tttataaaag gctgcttgcg gaaggtaaga caagagaaga agcattggat
     1321 atcattcgtg aaacaacgag agacaacagc aggacaccca tgcaatggac ttcggaacaa
     1381 tatgctggtt tttctacaca tgaaccttgg ctgatcacta atccaaatac caaaacgatc
     1441 aatgtagaac aacaagaata tgaaccagag tcggtcctgc aattctataa gaacatgatc
     1501 cgcattagac aaaccaataa aggtctgata tacggttctt ataaagaata tcttcatgaa
     1561 catccacaac tttatgtcta tgaaagatat ttagaagatg aacaatatct tattatggta
     1621 aacttaacag aatcacttgc tgactatgaa cttccaaaag aagcagatca gtcctggaca
     1681 ttactcttgt caaattcctc tagcggagaa tttgaagcta aaggcatact agcaccttat
     1741 gaagctcggt tatataaaac aaacaagtaa aacttcctcg ttaaagttca ttagttggac
     1801 tttgcgagga agtttttcag attgctctgt caattactca tttcaataat tcgctctagg
     1861 accaccgata tatttaggac gagagcgaag ggctgtcacg tgagcttgtc caagggtttt
     1921 caaacgaggt ctgacaggta cttggacatg tttgatatgc ataccgattg atgtgtcacc
     1981 gatatcgata ccggcttgtc caactacgtg ttccacttcg acaggatcat tgaactggtc
     2041 aaaagcagca acagaacaag cacctccagc atgtagtgca ggaacaacag agacaatttc
     2101 gaaatttttt gcttctgcca ctgttcgttc tactactaat gctcggttca aatgttcaca
     2161 accttggaca gctagcgcaa tttctttagg atccaacaac tcttttaacg tacggatgat
     2221 ccattgacca acttccgcac tggaattttt tccaatatgt cctccaacga cttcgcttgt
     2281 gctgcatcct aaaacaaaaa gatcaccttt tttcaagtgt gcctcagcca atatgtcatt
     2341 aactatttca gtcaattggt cttttaattc tttttctgac aacttcattt tctaaaacct
     2401 tctttttcaa ttaatattgc ttccgtgctt gaatacgttg aagacttgca gttataggaa
     2461 aggcaagtcc cattccaatc agattttgga agatgtttcc tggtatagaa gccactccag
     2521 ccggccaatc atataaaatc caaccggcga taccatagcc gatgatcata actaagcttc
     2581 ctaataaaag ccctaatccc cacatacgag tagaagattt gctgaaagaa ccagcaacat
     2641 atccttgtaa cccgtggatc actaatgaaa aaactgccca ctgcggatat cctgaaatca
     2701 aatcgatcaa tagaccactg gaagcaccaa caactaatcc acccgttgga ccataaagaa
     2761 aagcagcggt atagatcccg gcctcgcata atgtcacaaa tccattcgta gccggtacag
     2821 ggatcaaaat tgttaatgaa agggcaactg tcaacgcaat cagcatagca tagatcgtta
     2881 attgcttagt tttcatttct cattcctcct ttttgaatac cgccagacca gacacttccg
     2941 gtatgatcgt tcatcagcac gccattagaa atacaatggg caacaaattt tttactttct
     3001 gctactgctt tggtaaaatc tgcccctaac acaagattcg ctgttattgc agaagaaaag
     3061 gcacaaccgg ccccgtttac tgttgtagtg tcgatttttt tatgagaaaa tacctcataa
     3121 tgatcaattg aatagagaag atccacagct tcatctccag ccatccttgt gccgcctttg
     3181 atcacaatgt tttttgttcc tgaagcagtg agttctttag cgagatggat catttctgtt
     3241 aacgaagagg gaagagcttg accagaaagt aaagcagctt ctgccaaatt aggcgtaatc
     3301 atcgtaacaa gtggaaaaag ttcttcgatg agctgcgaaa tatacaattg attgatcaca
     3361 gcttcagttt ctttgaatgc caaaacaggg tctaaaatga ttgggatatt ttgttttgtt
     3421 tttatgaagt ctcgaacaac catgattgct tcacttgaat tcaataaccc aattttgata
     3481 ccatctaatg aagtcatttt atcaatcgaa actaactgtc gtttaagcaa ttccaaagat
     3541 atagattcca gcataaatcg ctcattaata tctagtgccc ctatagaagt caaggcgctt
     3601 agtccgaata gaccaaaatt ctcaaaagtt tttaaatctg tttggattcc tccaccagca
     3661 aatggatcag aaccgccaat tgtcaagatt cttttcataa cattttcctc catgaataag
     3721 agtataacga aattttcaaa aaagaaaaac agccaattgg catattttta tctcatccaa
     3781 tttaaaaaaa caaaaagaaa agaaagacca aaatggatct ttctttcctt aaatagttag
     3841 gacttttgtg attttaattc tttctcgatt tgaagccgtt gcactttcat tgtagctgtt
     3901 cgcgggattg catcaaaatc acggatgata gggacgttca agcgaggaag ttcatggact
     3961 tgctcccacc atcgattcca atccatttct ttatcaggag ctaaagcaat aatcggttgt
     4021 ggagaatgat ttttatccct tacaatcact acttctgcca aaaaatctaa cgaatctaat
     4081 aaaaagtctt caatagctaa tgtactattg atcgtttcga tcaaatccac ttgccgatct
     4141 ttcaaaaata ggtggccatg cttatccata aaaccgtaat ctccgctatc ccaccaatcg
     4201 ccataaacat tttcctggaa ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta
     4261 gacagtaatt gtatatgacc atctgtgttt tcaggtagga cattcccaac tgaatctgta
     4321 atccgagcgc ttgtcatgtc ttccagtcca acgcccatat ctcttgcatc acttgttttc
     4381 aaactctcta gtgtatgagc cttaagaatc attggtccgc attcactttg gccatatact
     4441 tgaagaaata ccgcatcctg tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg
     4501 tttatagcat caaaagtgga gtgatagtat cggatacccg aaaaagcttc tggatgctcc
     4561 ttagcagtaa aacgccactg aacaaagtta ttcggatggg tctctagtgc aattggccgg
     4621 tgagcttcca acatatgaac aacttttgaa ctttgtgcgt ttgctaaagg catcatagga
     4681 aatcccatgg acatcaaaga agagatacca atattgaatc tggaatgcac tggagagatg
     4741 tgaaaagcga ttaatttctt ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc
     4801 cgccatccca tagaatgagc agaatgacaa ataagtttag ggataccagt tgtaccagaa
     4861 gtatgggtca tataagaaat ttcatttttg tctaaaaatg attgagaaac agacaaagca
     4921 ggttgttcca ttaaataaag aatagg
//
LOCUS       DASJPH010000147         4939 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJPH010000147 DASJPH010000000
VERSION     DASJPH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.149"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U3995_002738"
     CDS             complement(<1..832)
                     /locus_tag="U3995_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1798013.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="U3995_002739"
     CDS             complement(846..1643)
                     /locus_tag="U3995_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1798014.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="U3995_002740"
     CDS             complement(1676..1960)
                     /locus_tag="U3995_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN1798015.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTLSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="U3995_002741"
     CDS             complement(1957..2958)
                     /locus_tag="U3995_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN1798016.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELFKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U3995_002742"
     CDS             complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U3995_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN1798017.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="U3995_002743"
     CDS             4026..4874
                     /locus_tag="U3995_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1798018.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1493 a         1079 c          798 g         1569 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgcca catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat aacgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taaataactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtg
//
LOCUS       DASJPH010000148         4812 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJPH010000148 DASJPH010000000
VERSION     DASJPH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.150"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="U3995_002744"
     CDS             complement(136..1098)
                     /locus_tag="U3995_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1798019.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="U3995_002745"
     CDS             complement(1100..2539)
                     /locus_tag="U3995_002745"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN1798020.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="U3995_002746"
     CDS             2724..4670
                     /locus_tag="U3995_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN1798021.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1539 a          816 c          783 g         1674 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aa
//
LOCUS       DASJPH010000149         4723 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJPH010000149 DASJPH010000000
VERSION     DASJPH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.152"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="U3995_002747"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="U3995_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1798022.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="U3995_002748"
     CDS             724..1878
                     /locus_tag="U3995_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1798023.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="U3995_002749"
     CDS             2093..3061
                     /locus_tag="U3995_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1798024.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="U3995_002750"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="U3995_002750"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1798025.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="U3995_002751"
     CDS             4091..4699
                     /locus_tag="U3995_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1798026.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DASJPH010000150         4466 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJPH010000150 DASJPH010000000
VERSION     DASJPH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.153"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(456..881)
                     /locus_tag="U3995_002752"
     CDS             complement(456..881)
                     /locus_tag="U3995_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798027.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="U3995_002753"
     CDS             1255..2226
                     /locus_tag="U3995_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1798028.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="U3995_002754"
     CDS             2258..3655
                     /locus_tag="U3995_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1798029.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="U3995_002755"
     CDS             3814..4437
                     /locus_tag="U3995_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798030.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1560 a          717 c          912 g         1277 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg tgaaatcagc gttttg
//
LOCUS       DASJPH010000151         4438 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJPH010000151 DASJPH010000000
VERSION     DASJPH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.154"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..3134)
                     /gene="brxC"
                     /locus_tag="U3995_002756"
     CDS             complement(<1..3134)
                     /gene="brxC"
                     /locus_tag="U3995_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1798031.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELI"
     gene            complement(3159..3734)
                     /locus_tag="U3995_002757"
     CDS             complement(3159..3734)
                     /locus_tag="U3995_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN1798032.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3734..4333)
                     /locus_tag="U3995_002758"
     CDS             complement(3734..4333)
                     /locus_tag="U3995_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN1798033.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1323 a          837 c          601 g         1677 t
ORIGIN      
        1 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
       61 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
      121 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
      181 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
      241 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
      301 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
      361 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
      421 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
      481 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
      541 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
      601 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
      661 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
      721 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
      781 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
      841 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
      901 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
      961 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
     1021 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
     1081 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
     1141 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
     1201 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
     1261 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
     1321 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
     1381 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
     1441 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
     1501 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
     1561 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
     1621 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
     1681 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
     1741 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
     1801 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
     1861 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
     1921 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
     1981 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
     2041 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
     2101 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
     2161 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
     2221 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
     2281 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
     2341 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
     2401 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
     2461 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
     2521 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
     2581 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
     2641 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
     2701 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
     2761 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
     2821 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
     2881 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
     2941 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
     3001 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
     3061 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
     3121 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
     3181 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
     3241 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
     3301 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
     3361 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
     3421 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
     3481 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
     3541 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
     3601 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
     3661 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
     3721 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
     3781 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
     3841 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
     3901 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
     3961 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
     4021 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
     4081 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
     4141 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
     4201 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
     4261 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
     4321 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
     4381 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattata
//
LOCUS       DASJPH010000152         4434 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJPH010000152 DASJPH010000000
VERSION     DASJPH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.155"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U3995_002759"
     CDS             complement(543..2048)
                     /locus_tag="U3995_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1798034.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U3995_002760"
     CDS             complement(2045..2731)
                     /locus_tag="U3995_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1798035.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U3995_002761"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U3995_002761"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1798036.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DASJPH010000153         4431 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJPH010000153 DASJPH010000000
VERSION     DASJPH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.156"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..429
                     /locus_tag="U3995_002762"
     CDS             4..429
                     /locus_tag="U3995_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1798037.1"
                     /translation="MNNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRE
                     NHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDK
                     GTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            445..759
                     /locus_tag="U3995_002763"
     CDS             445..759
                     /locus_tag="U3995_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1798038.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            785..1834
                     /locus_tag="U3995_002764"
     CDS             785..1834
                     /locus_tag="U3995_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN1798039.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            1846..>4431
                     /locus_tag="U3995_002765"
     CDS             1846..>4431
                     /locus_tag="U3995_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1798040.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSI"
BASE COUNT         1546 a          655 c          917 g         1313 t
ORIGIN      
        1 aatataaata atatagtgat agatttaaat gcatctgata aaaaaagtgt agtcgaaaaa
       61 ttatcgaaat tgttagagac taatgggttt gtgtctgacc acaaacaatt tcaaaaagac
      121 gtatttgaca gagaaaacca tatgaccaca ggaattggga aaaatatcgc cattcctcat
      181 ggcaaaagcc tagcagtaat tgaatcaaca gtggctgtag ccaagttaaa gcggtctgtt
      241 gaatggggcg cattagatga tcaaccagtt gatctcgtct ttttgttagc aataaaagac
      301 actgataaag gtactaatca tttacgtatt ttagcagaat tatctggaag attgatggat
      361 gacgagtttg ttaaaagaat aaaagattcg agaaataaaa aagaattggt aaaagctcta
      421 tcattttaaa aggaggaatt tcatatgaca aaaatagtag cagtaacagc atgtatcgca
      481 gggattgccc atacttatat ggcaaaagct aatttagaaa agtttgcgaa gaagaaaggg
      541 ttagatatta aagtagaaac acagggggct atggggatag aaaacaaact ttcaatttcg
      601 gatgtttccg aggcagatgt tgtcatattt gcagtagata caaatgtttc agaacgaggt
      661 cgctttgacg gtaaaccgat tttaacagta gggacctcag aggtagtaaa agatggtgaa
      721 gagacgattg atgaagcaat tgcattaatt aacaaataaa aaatatatcg tgggggagtt
      781 actaatggaa ttcaagaaac aaggaagaga aattctaaat catttccaaa gtggggtttc
      841 ctatatgatc ccgcttgtcg tagcagcagg gttactaact tctattgcag tcatttttgg
      901 tggcacaggt gtttgggacc agacggatac attctgggga gtcctgcgaa tgattggtca
      961 gacgggatta cagtttatcg taccgatgat ttcagcctat atcgcatatt caattgctga
     1021 ccgtcccggt ctagcaccag cttttatcac tgggatgatg tgtcaaaatc ttggtatggg
     1081 atttattgga ggaatggttg ctggattagc ttgtggctat ttagctaatg cattgaaaaa
     1141 agtaaaagta ccgatgcaag ttattagtct aaaatcaatt atgctgattc cttttgttac
     1201 tacattagtt gttggaatca tattatggta tgtactagct acgcctattc aagcaatgac
     1261 aacaggactg actaattggc tagaaggcat gtcaggagca aatgctgctt tgatgtcggc
     1321 tattttagga ggaatgatgg cgtttgatat gggaggacct atcaataaaa tcgcatatgc
     1381 ttttggggtt gcttcttttt caagcggaag ctttggacct agtacagcca tgttattagg
     1441 aattgctatt ccgccatttg gaatgttttt agcaacaatt ttaaacaaaa aactttatga
     1501 tgaatcggaa cgagataatg caaagacagc tatgattatg ggccttgtgg ggattactga
     1561 agggacgatc ccgtttgccg tgaaagatcc tttacgagta ataccaagca tcgtggtggg
     1621 cactgcagta gcatgtggtc taaatggatt ttttggtgtt actcaagaaa caatgctttc
     1681 aacctttatg gcaattcctt ttgttacgaa tataccttta tatttagtat caattgttat
     1741 tgggggagtg gttacagcgt taatggtcaa tgcactaaaa tccatcaagt ataaaaaaga
     1801 acaacaagta aattcagtag aagaaggaaa gtaggagaat ctgatatggt aaatgcacat
     1861 ataattcaac acacgcactg ggacagagaa tggtatttta caaatgaaga tgccatcgta
     1921 ttgtctgatc aggtctttac tgaagttcta gatgaattgg aaaggaaccc taaagtaaac
     1981 ttttgtttag atggacaaac ttcaatcgtc gatgagtatt tggagatcaa tccgcaaaag
     2041 cttaaggtta tccaacgact gatgaaaaca ggacaactat tcgtgggacc gtggtatgca
     2101 caaacagatg ccttattagt ggatgctgaa tcaattttaa gaaacttaat gattggaatc
     2161 agtgatacga tccttaaata tggagatcca atgatggtag ggtatttgcc tgacacgttt
     2221 gggttcaatg cgaatttgcc tacacttttg catcaagtag aaatcgataa tttcatgtgt
     2281 tggcgaggat taaactttga aacgatggtt ccatctcctt attttatctg gaaaggattg
     2341 ggagataaat ttgtttatgc catgaatttt ccatttggtt atatgactgg aatgatgacc
     2401 ttagacgcaa ttaaagatat tcctgaattt gcaaaagaaa agttggatga agcagttgaa
     2461 atgcttcata tgcaaggaaa aaacaaagat attttagtac cttcgggtgt agatcaaaag
     2521 tcaatgattt taaattttga tcaagtcgta gagaagttga ataaatacag taaattccac
     2581 aatattattt cagattatcc ttcttatgta gaagttatca gaaacaaaaa agaattacct
     2641 gaatataaag gtgaacttcg tcagccggtt tactctcgtg ttcatcgttc tattggctca
     2701 gtaagaacaa aaatgaaaat agaaaatttt aaattggaac aaaaaattct aaaacgagtt
     2761 gagccgctca tgctgattgc agaaagaaat ggtatccata tcagtaaagg cctacttcgt
     2821 cggttatgga aaaagatttt gcaaaaccag gctcatgata gtattggtgg ctgtgtttca
     2881 gataatgttg cggaagatat tttccatcga atcaaagaag ctaatgagat agctgacgga
     2941 atcgaaaacc ttattgtaaa acggatggct gatgcattaa agctaaaatc gaatgaagtc
     3001 cttgttttca acacaacgac tgagaattat agtggatcga aaatcgtgca tgtagtagct
     3061 gattcaaaga aaatacactt caaaggtagt ccctataata tcattgaaaa agaagtttat
     3121 tttccagaac ggaaaaatgc ttatcggatg attgctacag gtttcgaata ttttgatgaa
     3181 ccagcttatt atgaacttga cataaaaata aatgtaaatg tgccagcact aggatacact
     3241 gttgttgaat ttgagaaaaa taatgaaaaa ttagaaacag caatcgagat agacaaaact
     3301 gagataagta acaataaata taaactttct tttaaagatg ggcaattgaa tttgatagtt
     3361 ggagatagac aatatcttga tttcgttcat ttgatcgatt cagcaaatga tggcgatacg
     3421 tatgattatt caccattaga aggagataca gaactatctc taaaattaga aacagctaaa
     3481 gtatataaag attctttaca agaaacatta gttgtttacg gaaaagctca acttcctaaa
     3541 gatttaaaag atcgtttatc tgaaaaaccc gaaatggaag aaatatctta tgaaatatcg
     3601 ttcagtctgg gagaaagtca aatcgttgaa ggcacactca aaattcataa tactgtttac
     3661 tcgcatcgta tgcgcttagc aattaatgtt ttagataaat ctgaaaaatc aattgcacaa
     3721 attcaaaacg gatttattga aaatacccct gatgtcattc ctccgaattg gtcagaaact
     3781 atggtagaga aacctgttaa tttagaaata ttcgataaga gtgtctctgt ggcagaaaat
     3841 gatcattatt ttacgtttta tgcagatggt ttaaaagagt atgaacgtat agaaaatcaa
     3901 ttaatcataa ccttattttc aacaacaggg gaacttggaa aacctaatct tgcttggcga
     3961 cctgggcgag cttcaggaga tacgacaaat gaagggcatg tcatgatgga aacaccgcta
     4021 gctcaagaga taggtgagta taaagtcact tttggattta atgtagaaga aggaagattt
     4081 aatgagttca aagtggcaaa acaggcagaa aaacggttag aacaatctat ttcttatcaa
     4141 aagcagaaac tgaatgtatt tattcacagg ttagataaca agatttggcc attacaaaat
     4201 gaacttgatg ttcctcgtaa attttcattg attgaattac ctgaagactt aattgtatca
     4261 tcgatttatc cttcttattt tcatgaacaa ggatttgttc ttcgcttagc taatccaaca
     4321 gaacaagaaa aaatagtacc tgaggctatc ctttcccttg ggacagttgt caatgcactg
     4381 gagaataaac aagaattaac aacaattcct ccctatgatt atttgtcaat t
//
LOCUS       DASJPH010000154         4425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJPH010000154 DASJPH010000000
VERSION     DASJPH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.157"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="U3995_002766"
     CDS             690..1658
                     /locus_tag="U3995_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1798041.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U3995_002767"
     CDS             1669..2484
                     /locus_tag="U3995_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1798042.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U3995_002768"
     CDS             2552..3262
                     /locus_tag="U3995_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1798043.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="U3995_002769"
     CDS             3298..>4425
                     /locus_tag="U3995_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1798044.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASJPH010000155         4377 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJPH010000155 DASJPH010000000
VERSION     DASJPH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.158"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..234
                     /locus_tag="U3995_002770"
     CDS             73..234
                     /locus_tag="U3995_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1798045.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="U3995_002771"
     CDS             253..1530
                     /locus_tag="U3995_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1798046.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="U3995_002772"
     CDS             1540..2196
                     /locus_tag="U3995_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1798047.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="U3995_002773"
     CDS             2196..3572
                     /locus_tag="U3995_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1798048.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3858..>4377)
                     /locus_tag="U3995_002774"
     CDS             complement(3858..>4377)
                     /locus_tag="U3995_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798049.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DASJPH010000156         4363 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJPH010000156 DASJPH010000000
VERSION     DASJPH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.159"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(718..1011)
                     /locus_tag="U3995_002775"
     CDS             complement(718..1011)
                     /locus_tag="U3995_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN1798050.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1414..2592)
                     /locus_tag="U3995_002776"
     CDS             complement(1414..2592)
                     /locus_tag="U3995_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1798051.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2696..3010)
                     /locus_tag="U3995_002777"
     CDS             complement(2696..3010)
                     /locus_tag="U3995_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1798052.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3117..3332
                     /locus_tag="U3995_002778"
     CDS             3117..3332
                     /locus_tag="U3995_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798053.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3632..3874)
                     /locus_tag="U3995_002779"
     CDS             complement(3632..3874)
                     /locus_tag="U3995_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1798054.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3861..4289)
                     /locus_tag="U3995_002780"
     CDS             complement(3861..4289)
                     /locus_tag="U3995_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1798055.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1383 a          831 c          652 g         1497 t
ORIGIN      
        1 gtcttgtcct ttaatacaaa gattttattt ttaaataaaa aatactcttt ttttacgatt
       61 attgaaaaaa aaagataaaa cttgattaat tgaagtattg cttaaatttg gaagctatga
      121 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
      181 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
      241 aaacggtgtt caactagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
      301 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
      361 aataaaagca tatatattgt ttcaagaacg aaatcttgtt atctttttaa agggcatctg
      421 tccaaaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
      481 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
      541 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
      601 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaagt tcaagtatgc
      661 ggtgtagaac caatatcgca tacttgaact tttttcaatg agtataaatt aaaacttcta
      721 attttcaatt cccatcatct gatatgcccg accgtctgaa cttcttttga aaaagccaaa
      781 tgtgattaga taacgtcttt gttcgacata atcgccacta gaaaatacgt tcagcagtat
      841 cgaattaacc tctgtttctg tataagtcac tccatggtca aagcgtttca agacttcttt
      901 caataggatg tatttctttt ttgttgaact tggtacttgt tttacttttc catcattcat
      961 gaatgtgtct aaaactttca tcttttcttg ctccgtataa gtataaacca tcttctttct
     1021 ccttttgatt ctaagaatac tccatttatg ttagaatatt ctggattttt tattaaagcg
     1081 gttactctta agaaagatat tatcatagtg ctcttttttt tactactact tataggtaga
     1141 tttattctaa tccattattc tcccctacca ataattaaag aaaaaactgg atcagggtgt
     1201 tcaaattaca tgctaagaaa aaagaaccct tgaataccaa gggttctttc tcagtctgat
     1261 aacaatgatt aacgacggat ttctttgata cttgtgaaaa gtcgttaaag tattgatata
     1321 aaaggatttt tattacctct gactacatat tgactaattt gttaaaaaat cgtttttcaa
     1381 gaatcctgta ataccagtgc tttcaccgtt tttttaactc tctaaagaac taaaagcaat
     1441 cagaggtttt accttttcat ttgacgttaa gtggcgataa attttcgaag ttgtttggtc
     1501 agtatggcct aagcgatctt tcaagacttc ccagctacag tgttccgcta ctaagcttgc
     1561 cattgtatga cgagctaaat gagtatgaaa tggcttatta attcctgagc gattcacgca
     1621 ccgtttgacg tgatcattta catactctcg acgataagga acaccacgtt tactagaatc
     1681 aagatacaca aacaaatatg tttcttctat aataaacctg taatgttgcc tcatctcacc
     1741 caaagcctta atcaactgtt gtagcttttc cataactaga ggagttacta gaactcgtct
     1801 agcagaacta actgtcttag tagtttcaat tcgaaaatcc ttactcttac ctttaactgc
     1861 cgtttgctta tagaacagaa tttcatttct tttgaaatct atatctccag gctgtagagc
     1921 aagcgcctcg ctgactcggc agccagtgta aaatagaaca tggaaaagta cagacatcgg
     1981 taattcctca tgaacaatgc cataattcaa aaaagttctc aattcatcga cagtaagaaa
     2041 cttgtcctca atattattta tggcttcttt taaatcagtt accgttaact gatattttgg
     2101 cattctcaca gacttcattg gattttcact tatcaaattc tttctttgac aatatttaaa
     2161 aatcatattt agaacttgaa taagtgattg taaactattt ttggcatagc caacatttgt
     2221 tccattaacg ccacattcct ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa
     2281 atcagacaac tttttatcac caatccatcg aacaataatt tttaattgtt cctctcgctt
     2341 atcataggtt atttcctttt gttcatttaa cgaacggtaa tattggaacc attgctcacc
     2401 tacaacacta aacttgtcat tgcttgcaac aatttttttc ttaagccttt gttgtttctc
     2461 catttccaga taagcgagtt gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg
     2521 atatgcttga aagtaccatg tgatcctacc agtagaactg atttgcttct tgaatcgatc
     2581 tgttttttcc atttattgat tcctcctatt ttgaaggaac ccaacaagca gaaacccttt
     2641 ggaaatcttt ctatctatgt catcattgta ccgcatctta gaaagaaaat gtaacttata
     2701 aagatcgaag tagtcgcaat ttttctcgat agtcattcga atcactcttt ctcatccatt
     2761 ctaaaacatc atcacgaaaa tagtgtcctc gaatcccgta ccaacaggga ggaaactcac
     2821 caagttttac taaacggttc agtgtactat cacttatctg aaatagtcgc tttaaatcag
     2881 tatttgttaa taccagaggt aaatgactga ctgcaagcaa tttttccgtg taatcttcca
     2941 gttttttttg taaaattgaa tagaactcta tttccagaga attaacattt gttttaactt
     3001 ccaactccat cacccttctc tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt
     3061 gtaaaaaaac actccagtac tccatcaaaa cggactagaa aagaggaatt tttaatatga
     3121 aacaaggaat tgcagacata aaaataatta aagaaattct tgaaaaatct acggctaatg
     3181 caatagcatt cggaacagga atcaacttga gtacagtaaa aaaacttaaa agtggagaga
     3241 gagctgaaga aaaattaaat cttgccgatg ctattaaaat cacagaattt ggcatgaaaa
     3301 atatgccaac caaaatcgaa atatggaaat agtagttttt ctgatctatc agctgttttg
     3361 ccaaagcaca taggaatctc acctcccccc agtaatggcg ggccgatttg gttacggaag
     3421 tatcattatc gtagtcgcga ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc
     3481 atggcgtaac tagagaatca aaatctacat gtactgatag atttgctatt catttgtcaa
     3541 agagcgctag gctattttca gcctatggaa atcctttatt ttgacccgat aaaaaacttc
     3601 catagaatga aaaggaaagg gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct
     3661 tctcaatcaa ttttgtttcc agttgtctac gcatatattc atctacatac ataaccattc
     3721 ctttttcttc ttcataaact ggtttcatgg ctaaccgtga aatataattt tgatattttg
     3781 acagaacgat ggacatggca attgaatcac cagaagctgc ggctttgatt gtcgttagtg
     3841 gtatgaatgg tatatatcgt ttattttgct tcatatttga cacccaaata ctttcctagc
     3901 tcttccaaag ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg
     3961 ccaatttcac gatcattaag accgatgaag aaggacataa aaatgatttc ttgtctttga
     4021 ctaggtaatg ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca
     4081 aggacaataa aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct
     4141 aacccttttt gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc
     4201 ttttgaatat ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt
     4261 tgaatgtggg tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac
     4321 tcgttttact tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DASJPH010000157         4334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJPH010000157 DASJPH010000000
VERSION     DASJPH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.160"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..286
                     /locus_tag="U3995_002781"
     CDS             <1..286
                     /locus_tag="U3995_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798056.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            345..785
                     /locus_tag="U3995_002782"
     CDS             345..785
                     /locus_tag="U3995_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798057.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            797..1465
                     /locus_tag="U3995_002783"
     CDS             797..1465
                     /locus_tag="U3995_002783"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1798058.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1529..2152
                     /locus_tag="U3995_002784"
     CDS             1529..2152
                     /locus_tag="U3995_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1798059.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2208..2594
                     /locus_tag="U3995_002785"
     CDS             2208..2594
                     /locus_tag="U3995_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1798060.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2807..4198
                     /locus_tag="U3995_002786"
     CDS             2807..4198
                     /locus_tag="U3995_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1798061.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVATQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1442 a          723 c          925 g         1244 t
ORIGIN      
        1 aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt
       61 gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat
      121 ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga
      181 cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag
      241 aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt
      301 ggattttttt gtcataataa atattagatg atggaggaga taatatgatg caaacatggt
      361 tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga
      421 aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc
      481 gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat
      541 ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat
      601 atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg
      661 atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta
      721 aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg
      781 tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt
      841 tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc
      901 agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc
      961 tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg
     1021 gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa
     1081 aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg
     1141 atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt
     1201 ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc
     1261 cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg
     1321 tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat
     1381 ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac
     1441 aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa
     1501 agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac
     1561 attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg
     1621 tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt
     1681 gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa
     1741 agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa
     1801 agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct
     1861 gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt
     1921 tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag
     1981 aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg
     2041 agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt
     2101 ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct
     2161 ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa
     2221 gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag
     2281 aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag
     2341 aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat
     2401 ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg
     2461 tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt
     2521 cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag
     2581 aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt
     2641 ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat
     2701 aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt
     2761 tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat
     2821 cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa
     2881 gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg
     2941 cggtctgctt caattgatct ttggtgtaga cgacaaagtc gctactcaat caatggaatg
     3001 gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt
     3061 gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat
     3121 tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat
     3181 tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac
     3241 agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca
     3301 gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac
     3361 aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa
     3421 aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat
     3481 cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat
     3541 gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt
     3601 tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc
     3661 aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac
     3721 ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg
     3781 agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg
     3841 ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa
     3901 tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg
     3961 tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa
     4021 cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg
     4081 tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa
     4141 agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc
     4201 aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat
     4261 agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg
     4321 tgaataagag gttg
//
LOCUS       DASJPH010000158         4293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJPH010000158 DASJPH010000000
VERSION     DASJPH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.161"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..790
                     /locus_tag="U3995_002787"
     CDS             <1..790
                     /locus_tag="U3995_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1798062.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="U3995_002788"
     CDS             887..3136
                     /locus_tag="U3995_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1798063.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4293
                     /locus_tag="U3995_002789"
     CDS             3139..>4293
                     /locus_tag="U3995_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1798064.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1392 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact aga
//
LOCUS       DASJPH010000159         4212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJPH010000159 DASJPH010000000
VERSION     DASJPH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.163"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(30..1250)
                     /locus_tag="U3995_002790"
     CDS             complement(30..1250)
                     /locus_tag="U3995_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN1798065.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1270..1881)
                     /locus_tag="U3995_002791"
     CDS             complement(1270..1881)
                     /locus_tag="U3995_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1798066.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1924..2856)
                     /locus_tag="U3995_002792"
     CDS             complement(1924..2856)
                     /locus_tag="U3995_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1798067.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3277..4137)
                     /locus_tag="U3995_002793"
     CDS             complement(3277..4137)
                     /locus_tag="U3995_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798068.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1197 a          852 c          690 g         1473 t
ORIGIN      
        1 tttatttaaa actttgcaac agaacctttt tattttacag aagcactcga aactgcatct
       61 actgcagttt catcgtgtgt gctggcgctg gttactgcat ctgtttttcc accatgaaca
      121 gatgcacttg aaaccgcatc ttcatttcca acaagttgtt gacccgtttg ttgtaaatac
      181 cactcaatga ttggtttgaa atcaagaatg tattcattga ttccagcata ctctccattt
      241 tcatcatgca ttgcttggta attatgcaca atatattttt ctggcacttg ttttggtaca
      301 tgaacacgta ccgcatctgt tgctccgctt cgcagttgtt gaattaccca ctctacattt
      361 ttatacgatt tttctgggtg acagttggct aatggattcc ctacctgacc gggcacacga
      421 gcagctaaca tctctttcgc ttcttggcgc aaattataat ataaaaattg gttattatta
      481 tctgcaaaag ttaattccat cggcatcgaa tctaaaaagt aattgatttg atccactgtc
      541 aataacccac gatttaattt gacgtaagta tttccagaaa cggcaccgac tgcttcagaa
      601 gctttttcca accaatgatc atctgacata tcaacatcgg gtattgtcgt agcaattgtt
      661 cctgtcgcat gcaaatcttc tggtggaagc tttttaggcc ctgctaaggt agacacgact
      721 ttaatgaatt ttacaaaact tgctaatgta ctgcctaaaa agtcaacagt tcgtggatca
      781 atcaagcgat cttcttggtc aaatgcttct tttgcttttc ccaataaaaa ttcatttccg
      841 ggcatcacta atgcattcac gcctggtgcg tccaaaattt ggcgcaagtg taattgcgcg
      901 cgtgaagttc cttgatcgta gtaagaagca cccacaacca ttacaggttt attttcaagt
      961 ggatggattt taaatgatag ccattcaagt gcacttttca atcctgctgg aaccgtatga
     1021 ttgtgttctg gcgtagcaat aattacccca tcagccatta agattttgcg atttagctgt
     1081 tgaatagcag gactatttgt ttgatcatca tcttgattga acagaggtaa atctttgatt
     1141 tcaatgattt ctaaattaaa tagttcatga tattgttttt ggataaatgt taataattta
     1201 cggttatatg aaagttcagc tgctgatccg acgataccta ttaatttcat ctttgtttgc
     1261 tcccctcgat taaatcgttt cccatgaaaa gctttcggct tcacgtttat ttgcttggtg
     1321 cgcatgttgc aattgactag tgattgtgac aaagagtaag aaatctttga atagttcacg
     1381 taattttgcg acttgttctt catcaatgag tcgtccttgc tcatcaaagg cttggagtga
     1441 atgacccaat aaaaattctg agcttggcat aattcgtgcc tttaactctg gtgaatccaa
     1501 tatttggcgc aaatgtgctt gtgcgcgtga agaacctaac gtgccataag acgctccggt
     1561 aatcatcact ggtttatcca caaacggaaa aataccatat gaaagccaat ttaatgcgtt
     1621 aatcaatgaa gcaggaaccg catggtcata ctctggtgtg ctgatgatta cgccatctgc
     1681 ttgttcaatt tttccagcaa tttctttaac aacatttggt agttctgtgt tttcaggttt
     1741 gttaaatagt ggcagctcct tgatttcgac aatttcgatt tctgcctcat ttacaaaata
     1801 atttttcata aactgtaata attcacggtt tgttgaccgc gttgaatttg ttcctactaa
     1861 tccaataaat ttcatcttca ttctctttcc tttctaacat cgatttctga ctgttcgcac
     1921 gtactaaatt ttttcaaatc cttttgtata caagaattga ttctcttttg ttacaactgc
     1981 tccctcgata cccggctgtt cttcaagcgc tttcaacact tctaaagaag atgctccaaa
     2041 taaacgtgta gtccagattt ctccatctac agaagaatca gacaaaatcg tcaggcttgc
     2101 tacgtcattt ttaactggaa agccagtttt cggatcaatg atgtgatgat acactgacga
     2161 accattttgt agataacgtt cgtagattcc agaggtaact aaagattggt tgtttagttt
     2221 aaccaccgca agcgactctt cacgtgaacc tttaggattt cgcaaaccaa tacgccaagc
     2281 accatccgaa cgtcttggtg attcaccaaa aacaacgata tttcctccta agttaactaa
     2341 agcaaacgct gcatttcgct ctcggcaatg tgtaattaat ttatctgcaa tgtaaccttt
     2401 tgctaacgcc cccaagtcaa tcatcattcc tggtttggtt aaaaaaacgg ttttcatttt
     2461 ttcatctaat tgaatttggt gtggatccgt tagcagaagt aactcagtta tctctgcttt
     2521 tgttggaacc aatgcatctt caaatccaat gtgccatttt tgtacaaccg gaccaatagc
     2581 aatattcaga taactatttc ttgcacaact atgtgctttt cctaatttga tgagctcaaa
     2641 aagttcagga tgtacaatca cagcttgtcg tccagcagct tgattgatgg ccattaattc
     2701 agaagttgca tcatgtgcgc tgaagcgatg ttcataaacg tgcaaccatt cgcctaactc
     2761 tgctagtaat cgactaggct cttcatgccc aacttgtaat gtaattgttg tccccatcat
     2821 ttgccagatt ttcgtttctt cgatcacctg attcattttg taccttcttt acttgaacta
     2881 cggattacat gagtaattag tttttatttt tgttactgtt ttcacaacca gatgatacgc
     2941 ctgctagatt tatttgtcaa ccgattttat atattttttt atataataag gtaattttaa
     3001 tataaaattt tatattgtaa aaaaacaaat aagggaaaca aacaacacat ttttgtgtgt
     3061 tgcttatttc cctctaacta tcgttttcta tttgatggtt aacctactta aaataaatac
     3121 tgcaacgaca aacggagatc aagcatgctt tgtaacgtat tttacgataa ttacgacttt
     3181 attttattac tctatcttgc tgccataccc gtctatccct aatcatcttt ggtgaatttt
     3241 ttcaaagtta gcataacaaa aataagtatt ttttccttag ttaatcaata gtatcatttt
     3301 tttcatatga tttaattcat caattaattt tttgttttta ttaattttag caaaagctag
     3361 tgcatcttga atcaattcga gcacttctgc cttcgactta tttttagcct gtgcgttctt
     3421 cgctaattgg gcagcagctc gtgctaagta ataagtcaaa tggttatccg aacaaatatc
     3481 gattgcgtaa tttagtagtg catcacttgt ttccaaatca ccgtcttgcg caaaaaaaac
     3541 accacaatga aagacgatat ttaacacccg ccagatatct tctgttgtac gaatcggata
     3601 cgtataaatt ttttcaaaca ctcgattgaa ataaacttct gctttttcca actctttttc
     3661 tctagcatag accattccaa tacctgtata tgccaataaa cgaaacaacg tgttggctgt
     3721 ttctgccatc aacaactgat caaaatggaa caacacatcc gtaattggtg cattcgtaaa
     3781 aatcatttca aatcctttta gatagtagta acgtgcaatc agctcagact cgtcaattac
     3841 cttgggatca atggctgcta aaatctgtgt caattgttca tattcactcg taatcagtga
     3901 aaactctgct ttttctagct gttcatcaag ttgtgtattg atcacgttca tcgaagaaaa
     3961 aagttcaact aacggaagat tgattcgatt acataatttg attaaaattt ttaacgaagg
     4021 gacttgacca ttgttctcaa atttacttaa tgttgcttgt gtacaaattc cttcagacac
     4081 ttctttttga gataagttgg cagcctttct tgccgtaata aatttttcaa tgttcattat
     4141 tttctctcca atcagacctc acacgaaaat catttctgtc tcgattttag catgttttct
     4201 ttgggaatgc tt
//
LOCUS       DASJPH010000160         4157 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJPH010000160 DASJPH010000000
VERSION     DASJPH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.164"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..775
                     /locus_tag="U3995_002794"
     CDS             179..775
                     /locus_tag="U3995_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1798069.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="U3995_002795"
     CDS             899..2698
                     /locus_tag="U3995_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1798070.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="U3995_002796"
     CDS             complement(2976..3188)
                     /locus_tag="U3995_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798071.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="U3995_002797"
     CDS             3650..3799
                     /locus_tag="U3995_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798072.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4052..>4157)
                     /locus_tag="U3995_002798"
     CDS             complement(4052..>4157)
                     /locus_tag="U3995_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798073.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1229 a          707 c          728 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gagatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DASJPH010000161         4073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJPH010000161 DASJPH010000000
VERSION     DASJPH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.165"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..392)
                     /locus_tag="U3995_002799"
     CDS             complement(<1..392)
                     /locus_tag="U3995_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1798074.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIA"
     gene            complement(502..1071)
                     /locus_tag="U3995_002800"
     CDS             complement(502..1071)
                     /locus_tag="U3995_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1798075.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(1089..2504)
                     /locus_tag="U3995_002801"
     CDS             complement(1089..2504)
                     /locus_tag="U3995_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1798076.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(2515..3183)
                     /locus_tag="U3995_002802"
     CDS             complement(2515..3183)
                     /locus_tag="U3995_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1798077.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(3313..3897)
                     /locus_tag="U3995_002803"
     CDS             complement(3313..3897)
                     /locus_tag="U3995_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1798078.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT         1420 a          724 c          525 g         1404 t
ORIGIN      
        1 gcaattgcca tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa
       61 gaatttaaat caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc
      121 aaattagtct ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat
      181 aaagctccta aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca
      241 tctcctacaa taattagaga ctgaagaaat ttgtaaatac tgtaatatcc atagactgtt
      301 aatacgacag aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg
      361 acataactaa ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc
      421 tcttattcgt ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac
      481 agataaaagg taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa
      541 tacaccaatc atagataaac ttgctacaac taacactgaa atgattcccc atatacctac
      601 cgcatagtgg atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg
      661 acgaaaacga tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg
      721 cattagagcc caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat
      781 aatatcagct aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc
      841 cattaacgta aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg
      901 gtagttatag ctaatataaa caaatatgat tccagataat aatccaaaaa tcccggggga
      961 tatgctgtga ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac
     1021 tagggaaata actgtagcta aaccaataaa aatataatct tttgttttca tatatattct
     1081 cctcaattct atttcgttat gtcattaaaa atctgttcta tcaaagttga atcttctact
     1141 ctaagtttta tatctttttt tattccacca ttttttaaat aaataattct attgcatgtt
     1201 ttataaagaa attccggatc atgagtagct ataactactg cacccttttc ttttaagtct
     1261 ttaataaggt cagcaatcct ttccatatta atataatcca acccacttgt aggttcatca
     1321 aatagaaata aattagtttt tgacaatgaa gctatagaaa ccaataatct ttgtttttct
     1381 ccaccggata aatcaaacgg taattgatct ttcattttat aaagttccat tttttttaaa
     1441 attttttctt gctgatcttt actaactatt tcattcccca gggctaactc gctcctcact
     1501 gattctgtga aaaattggta atccatttct tgcattacta aaaacggcga tgacaaattt
     1561 tttttatttt cgattatctt tcctttggta gggcgaataa tcttcatgag tattcttagt
     1621 aaggttgttt tccccacacc gtttttccct accaaaccaa taacatctcc aactttgaca
     1681 tcaaaattta tgtcttttag aatattttca taagtaattt cttcaacttt taatacatct
     1741 gcacttacct tttcgatata gggaacttca atcgaactat taaaaatgtc aaatttcctt
     1801 aatgcgttat tgttttcagt ttttaacaac tgaatagttg tagtttttac agttctattt
     1861 ttaacaaata gaacttgatc aattaattct ttcaaatagt aaaaacgatg ttcagctata
     1921 acaatagtaa atcctttatt ttttaaatca caaagtaagt cagcaagttt cttagtcgct
     1981 ttgaagtcca aatttgaaga cggttcatcc aatataatta atttagagtt tagcgataaa
     2041 ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat gtttttatct
     2101 aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc catatgctct
     2161 tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt tgttgtaaag
     2221 aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt
     2281 aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt
     2341 cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca
     2401 gtaatcaatg taagactgcc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc
     2461 ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat
     2521 caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat
     2581 gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt
     2641 agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc
     2701 caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt
     2761 aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa
     2821 cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata
     2881 gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa
     2941 ggatgtcgca aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac
     3001 taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat
     3061 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
     3121 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
     3181 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
     3241 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
     3301 agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct
     3361 tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat catagaatgt
     3421 agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta aattttgatt
     3481 gatcattccg atagtcttta acttatctag acaacagact gtctttttcg tcacaaatga
     3541 tgtaattttt tcaacaatat cttgatattt ggttccagct gattggaata aaatcaaatc
     3601 gaatatacgt ttattctcaa aaatataatt aataaatgga tctaattcat tgaagttctc
     3661 tctccaactt ttctctatat ctatattttc taagtttaag aggtatttgg taaactcttc
     3721 attatacata ttaataagcg cattaatatg cggctgaata atttcttcaa ataacgcttc
     3781 cttagaagta aaatgtttat aaaaagcacc cgtagttacg cctgcggtca ggcacagttg
     3841 ccttatctta gtttgctgaa atccttcttt ttcaaaatgg cttagtccac tttctattaa
     3901 cttttggtga gttataatat aatctctggt ttctatgggt acttcctcct aataagatga
     3961 attaacttta ttatcttaca caggacttga agtttcaatc gttaacgttt attactgaac
     4021 tcattcctta gagctaaact aatgagaaag agcagaataa ctgatacaaa aag
//
LOCUS       DASJPH010000162         3901 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJPH010000162 DASJPH010000000
VERSION     DASJPH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.166"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(145..915)
                     /locus_tag="U3995_002804"
     CDS             complement(145..915)
                     /locus_tag="U3995_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1798079.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(908..1843)
                     /locus_tag="U3995_002805"
     CDS             complement(908..1843)
                     /locus_tag="U3995_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1798080.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2159..3808)
                     /locus_tag="U3995_002806"
     CDS             complement(2159..3808)
                     /locus_tag="U3995_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1798081.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         1176 a          875 c          685 g         1165 t
ORIGIN      
        1 cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
       61 caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata ctcaggtcaa
      121 aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat tgatcaaata
      181 ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg atgttgctac
      241 tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat aaacgatcgg
      301 attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat agaaaacgcc
      361 acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat cgtcataaga
      421 ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg tcatcaataa
      481 tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca aggaaatgat
      541 cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa agccccaaat
      601 gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct ggaatttttg
      661 agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca tcataatcaa
      721 accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc gaccaccgat
      781 catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg
      841 gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta atgcagtaaa
      901 ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt ttcaaaaaca
      961 gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct tgtcttgtca
     1021 gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg attgtctgtt
     1081 catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat ggcgctaaat
     1141 caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta gtattctcga
     1201 tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct tcttctaaat
     1261 aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga aactcccaca
     1321 tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc agcaaacgtg
     1381 gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg gaaagcatac
     1441 gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt tcgctgcgtt
     1501 tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg atttgctgga
     1561 ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt tgctttgctc
     1621 ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat gttttattga
     1681 ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt cctaatagcg
     1741 catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg accccacttc
     1801 cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc ctatctcctt
     1861 atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa cttggttcca
     1921 gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc aatcggacga
     1981 agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat tcatatgtgc
     2041 aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     2101 aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat cacttcgcct
     2161 atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt tttgcggttt
     2221 ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt cgggtccttg
     2281 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
     2341 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
     2401 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
     2461 tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag cagcgttctt
     2521 tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca aaataactgt
     2581 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
     2641 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
     2701 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
     2761 agttgaccgg attcactacc gcttctttta acaatggatg atagatatct ttcgctgtca
     2821 caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg taattcaata
     2881 ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct tcagttgaat
     2941 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
     3001 tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat gaaaaaccta
     3061 atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga agtggtgtcg
     3121 gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
     3181 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaacccac
     3241 cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa cccaccagcg
     3301 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
     3361 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
     3421 ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct aactgctgat
     3481 cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct acactagaat
     3541 aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa gtaagatcat
     3601 cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc ttcaagcttt
     3661 cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga actgtttcct
     3721 ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct gcaataataa
     3781 ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt gttagcttta
     3841 gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt
     3901 g
//
LOCUS       DASJPH010000163         3863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJPH010000163 DASJPH010000000
VERSION     DASJPH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.167"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..466)
                     /locus_tag="U3995_002807"
     CDS             complement(245..466)
                     /locus_tag="U3995_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798082.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="U3995_002808"
     CDS             complement(564..809)
                     /locus_tag="U3995_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798083.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="U3995_002809"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="U3995_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="U3995_002810"
     CDS             complement(2033..3034)
                     /locus_tag="U3995_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1798084.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="U3995_002811"
     CDS             complement(3193..3459)
                     /locus_tag="U3995_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798085.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="U3995_002812"
     CDS             complement(3449..3805)
                     /locus_tag="U3995_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1798086.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DASJPH010000164         3836 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJPH010000164 DASJPH010000000
VERSION     DASJPH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.168"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..371)
                     /locus_tag="U3995_002813"
     CDS             complement(<1..371)
                     /locus_tag="U3995_002813"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1798087.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDG"
     gene            complement(374..2005)
                     /locus_tag="U3995_002814"
     CDS             complement(374..2005)
                     /locus_tag="U3995_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1798088.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2309..2980
                     /locus_tag="U3995_002815"
     CDS             2309..2980
                     /locus_tag="U3995_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1798089.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1258 a          705 c          512 g         1361 t
ORIGIN      
        1 ccatcatcta aacgataatc caaatccgat tttacccaat caaatactgg cataaaatta
       61 taacagataa cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt ataattctca
      121 atgtattgat ctcgtgttgg taaaccaatt ttgatatcat catgaacatt aacactctca
      181 ataactttca aggtaagtcc attggcttcg acttcatctt ttaaagattt aattcgttct
      241 ctcggccaaa cctctccggc tggtatatcc attaatgttc caacaattcc tttcattgct
      301 gggatctgac gaatttcttg tagtgagatt ttttcttcat ttgccccata ccatctaaaa
      361 gtcatttcca tagttacttt cctccaactt ctgttatata tttattgatt gttttacgaa
      421 ctgcttgagg acctactagc atttcgcaaa agattttttc agttttcttt cctaccccaa
      481 cttgatataa atctaaacca aagatttctt tattttctaa aatttcctgt accgcacaat
      541 gaatctttga aggatcttgt tcacctaatt tcaatactga taatttatcc tgcaactcta
      601 ccaataacgg atcaggactt ggcacaaaat tttccccttg atcatcgact gccagtaaat
      661 aacgtaacca agcagcaata gcgataggaa taaatgttag attttctgca tttccgtctt
      721 tttcgacata ctttttaatt gtttcaccat agcgaatcgc tactttttga gaagtatctg
      781 ttgcaattcg ttgtggtgta tctggaatat agggattagg caaacgttta ttaattactt
      841 catcaataaa ttgttttgga tcaataatgc ctggattttc aactactggt aaaccttcta
      901 aataacctat tcctttgatt aaaccaacca aatcatcatc ctgcatctct gctgaaattg
      961 attgatatcc tagcagacaa cctgttactg ccaaagcagt atgaagtgga ttcaagcttg
     1021 tcgtaacctt catgatgtct gctttatcaa ccgtctctct atctgtcata atcacaccag
     1081 ccttttccaa tttaggccgg ccatttggaa aactatcttc aataactaaa taatgaacta
     1141 cctcagtatt agtgaaagca gcaatattgg tatgtttttt tgtgtgaatg atatctaaat
     1201 cctcgagccc catcatcaat agatcttcct ttacattctc tgaaggattt ggcgtaattc
     1261 gatcaatcat actccaagga aaagtcactt tttctttatc ctttaaataa gcaataaatt
     1321 tttcagaaac atatccttct ttttgccaag cttctgcaat cgtaaaaata ctatcttgaa
     1381 aacgttgacc attctgtgag aaattatcag tagaaaccat cgcaatcggc aactctccag
     1441 cattaaatct gctatataac aaggatgtta caatactcat cgtatgttga ggattgctag
     1501 gtccagattt gatatcagct ttgactaccg aaaagaattg ccctttagaa tcttttaaac
     1561 tgtaaccttt ttcagtaata gtaaacgttg taaactgtaa tttaggatct tcaaaatatt
     1621 taaccactct ttgatagtcc gctatattct tcggattgca atataaacta tcagccgtta
     1681 ctgccaaaag tctttttttg agttcaccat ctccactcat aacaatttcc aaaatgtcat
     1741 tgttatagtc atgataagct ttttccacta cttcttcatc aaatgtttca cacacaacta
     1801 ctcctgtttc taattcattt tgatctgcta agcattgagc aatttctgca tgaaatcctc
     1861 gataaagatt tcctccacca aaatgaagcc atactggatg ttgctggcct tcttgcttaa
     1921 cttctttgac atcaaaacac ggcagtagaa tctgcatttc attgaattct tcgatagaat
     1981 tttgccaatt tttaatcgaa accataatta acctcctcaa actgatatgt tagttttgat
     2041 acattttata ttttaaaacc cattgtaacc gttgtcaata taaaaaggga ataaacttgc
     2101 ttttttctac catatcagaa tgattgtatt ttttattcag aaaaataaaa tactccttct
     2161 ttttggactg attttatcga aacttatttt ttcttttttt catgtattct tttattttta
     2221 taactagtgt attacttcaa aatttgatat tatatggcat gaacagaatt aaaatatcag
     2281 ttaaaataaa agttaaggag cattgtgtat gacaattaaa actagtctcc aaaatcaagc
     2341 ttaccaatct attcgccgac aaattatcta tgctgactta gaacctggaa aaaaagtatc
     2401 agaaaaaaaa ttagaagaaa cactttctat tggtcgtaca ccaatacgtg aagcactgat
     2461 tcaactccga caacaaggtc ttgttgatac aattcctcaa agcggaaatt atatctcatt
     2521 aattgattta gattcggcca aaaatgctcg atttgtcaga gaacatttgg aacgacaaat
     2581 tatgttagag tgttgtgcca aaatgaataa ccaaaaaaaa cggattttag aaactataat
     2641 ttcagaacaa gaaaaagctg ttagtcaaaa aaatgctcgt agtttttttc ataatgataa
     2701 tttattccat aaagcttgtt ttgaaattgc agagcgagaa gaagtatggc aatggttaga
     2761 aaataacaat acacacttag aacgatttcg atggctacga gtaaaaacgg aaggattaaa
     2821 gtgggaaaaa attatggaac aacattatca actatttgag gcattaacaa aacaagatcc
     2881 agaagaagct aactttctaa catcgttgca tcttcattta atgcttaatg agcaaaatat
     2941 tgttttagct actcatccaa aatatttcaa aaatatataa cttttaactc tttttaaatt
     3001 aaatggttgt tctgatcaga aaaatatcgc ttgttttctt attatgtaat atatttcata
     3061 aactattctg cataacaact attgtttgta ctttttacat tggataacta attatgactg
     3121 ctgataactt ttgatattaa actagtatct ccaatacaaa taaaaaatag atatcttgtt
     3181 catcaaaatt agttcgtgga tacttacctt catttacatc attcagcggt ttcccatttt
     3241 cttttattct attatttcat tttctaatag acttggcttc atttcgcaat actttttttc
     3301 aataggcatc aaattggtgt tgtttagata tttagttgta gttttgtgac atgataagta
     3361 tgctcctttt tctcttttcc actaatacaa cgatcaaagg gtgctttttt caaaaaacta
     3421 gaaaaatcaa aataagattc gtattttgat tctatatatc ctgtttcaac tacatcttat
     3481 atttcacaat agaataaata gccttttgca tagctgctta acttataaga aattgcaatt
     3541 tacttcttca aagtgaaaac gaatatgagt aaaataaaat tattcttctt acagataatt
     3601 aaaaatagcg cgtaacaaaa atcggcattc aatcacataa ttaggattga tgccgattac
     3661 ttatattcta cgtatagttt tagtattctt attttttatc accagtaacc aattgagaac
     3721 agccctaaag cagaatagtc ttttatctca aaacgcttaa tttcaggctt aggaatatga
     3781 ataatctttt gagtatattc tttctctttt aatcttactg aagaaatacc actgcc
//
LOCUS       DASJPH010000165         3765 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJPH010000165 DASJPH010000000
VERSION     DASJPH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.169"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(610..1167)
                     /locus_tag="U3995_002816"
     CDS             complement(610..1167)
                     /locus_tag="U3995_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798090.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1346..1957)
                     /locus_tag="U3995_002817"
     CDS             complement(1346..1957)
                     /locus_tag="U3995_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798091.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2070..2327)
                     /locus_tag="U3995_002818"
     CDS             complement(2070..2327)
                     /locus_tag="U3995_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798092.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2924..3202)
                     /locus_tag="U3995_002819"
     CDS             complement(2924..3202)
                     /locus_tag="U3995_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798093.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3236..3487)
                     /locus_tag="U3995_002820"
     CDS             complement(3236..3487)
                     /locus_tag="U3995_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798094.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1128 a          775 c          510 g         1352 t
ORIGIN      
        1 ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa atggtgtcca
       61 aaagcttgca tctgccagat tatctcattt cttatgacaa gctaagcaaa ttatctccac
      121 caaataattt attcatattt ttgacttgcg catactaact aattcttaaa ttttcataca
      181 aaatttctca atttccctta acgagtaata tttatgtacg gtgattctca ctagtatccc
      241 aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc ttttatgtat
      301 gctgttagtt gattagatgg tcatttgttg agctaggtcg tttattttaa acaagttgtt
      361 tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa atgcatacgc
      421 atctgtattt gccactttga ttcgtataaa gctaacaact attaactcct ctcagctaat
      481 agttgctctc ttttttattc agattcattt ttgctgatca cagcctagta tttcaatggt
      541 tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa atggattagt
      601 agatttcgat tattcaaagc gattttctat tggtggatcg tgttcccaac taataagacg
      661 gattccaaac ctatcgtaca gatcttctaa accacttata aattttgcac ctatttcagg
      721 tgttttattg cgtggatatt ctattgttac gccattaaat ggataccaat taggattagg
      781 ctctcttttt ttctgtacat ctcccataga atagttagca ctttctggga cataatatat
      841 tcttcgacca tcctctaatt catctaataa gatatttcgt gatcgtctat cacttgctaa
      901 atgtagacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa atccatcaaa
      961 taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat attctaatct
     1021 aaaatcaaac tcccctcggg taatttctgg tcttggtggc ttaggaccca atattattct
     1081 acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa tgattaatat
     1141 tttgtttcta tttgataaat ttctcattat tcgcacatca cctctacatt ctcacatatt
     1201 agttcgtaat actcctttaa tatcaaaata tctactggtc gtaataatat tcgctatgaa
     1261 ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga tgatttggct
     1321 tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc ggttcagata
     1381 tttcccagtt gattaaggta attccgtatt gtgtcaaaac ttcttgcgct tctccgaaat
     1441 taagtacagt tcttatgtat ccactttctc ctaatataaa gtctacctct cttttaaaac
     1501 cttcctcgag atttctaagg gctatatgtg gttttaatga cattgattgt acattttccc
     1561 ccatataata ttgcgcactt cctcgactaa aatagattcc tagatcgtcc ccaaaaggta
     1621 gatcttctct accactagct aatctacttc tccatcttcg ttctcttttc atagaacctg
     1681 cgctgaactc acacccgtta aactcagcta tgacagtatc ttcaattatg ttaatctgtc
     1741 catctagttc gtattcaatc ctaaatggaa attctccata ggtgatttcc ggttcgggta
     1801 ttttaggtga gattaatgca aagaaaggaa atattaagct tggcaacatc aaaaaaactc
     1861 ctcctatgat cacaattccc gatgtaatcc aaaatataat ttgtacaatt ctcattgatc
     1921 cattgtattc cattttattc cccctattac tattcatatc cgcctatcta tatcgatctt
     1981 ccaatgcaga gataattgct tccatacctt gattttttac tgagttacct atgcgagata
     2041 tcattctcca ctaaggtgca tcatattgtc tatctaacat gattaattct ccaataggtg
     2101 tatactcctc caaaaacaac atttaatgca tctccctcga caacataagc taccatgttt
     2161 gctgtatatg tgtttgagtc taattcataa gcagataaat ttactgtcgc tggattaaat
     2221 ataatcgcat tcgtatttat agctactgaa tttgctgctt cagcaccacc ttttgaatgt
     2281 ccgatcattg tgatttcaat agtaggattt acgtggacag ccctcaccgt ttaccctgac
     2341 cattatagaa atcgcttact tttttgaact acgcaacaaa ggatacaatg ctcctaactt
     2401 cactgtctcc atttttgtcc actaaggaat ataaaaaata ggcattaatt catttaattc
     2461 ggtcacaaca cgttaccttt taaatcatta tactgtatct catacattat tatcaataaa
     2521 ctattataaa aatgcaaaaa atgctttatc aatctatttt gtaattggtc ataatatcat
     2581 tgaaatcata ccctgtatcg atttttccta tgaatctcgt ctatttgtga ttgacgaaaa
     2641 agaggcaaga cagacactgg aatggtaatt ttacccacta ttttgctcca actatttgta
     2701 atcgtaccca cttttgaaat accgagatga tcattttaat tgatatgctg attcccaagc
     2761 aaaaccccaa gaaattaaat gctctatcgc atctagcttc ttgggttttg cttatataaa
     2821 ttccaacatt taaatttttt atgctgttcg gtctcagaaa tgccgttttt gcttatatca
     2881 caatatcaag atgtcaaatt attcgtgttg ccgatgactt tgattaattt ttttcaaatt
     2941 gtttattaag actaatgctg tctttttgca gataccaatg ttcgaacttt ttaacattat
     3001 catatgatcc tgcaagtttt aacttcttac cattagatgt tatcaaagtt ataattaacc
     3061 ttgccttttt cccattagga tcttttattt tatgaatttt ctgcaggtaa tctctcctaa
     3121 tggcagctat ttttacctca tcagtgctga taaaccattg atcatccaca tacatactgc
     3181 ctgttatgtt gtgtttttcc atttcttccg agaacgaaaa gtttaaggct cgttcttact
     3241 tccaaagttg aatgagtcca tggattggac ttagtgtgag aattgagaac aacaataaga
     3301 atccatatcc tcccgcaaac atccctaagg ctatactgtc tctccatcca taagtaggat
     3361 tagaagagaa aagagtcacc gtttgtaata atatgaaaag gaataatcca gcacaaacac
     3421 caaagataac aactccaaac attgaatttt tatacaatga ccatttattt ataggctttt
     3481 ctttcatttt acttccccct actgttctgt tcacacggaa tgaattcaaa gttcataagc
     3541 cctcctcttt gaacatatga atacgacttg ctaactatac tttaaaaaag cttgttttaa
     3601 gcggttatag gcgagttgta atctctattc atctcattag caaataactt gccgaaaagt
     3661 ctactgaaat taaagcagat agtggaagaa ttatttagcc aggatagaca ctatttgtga
     3721 aacacatgtc tttacttttg aaattctatg atgaaaagcc ttgga
//
LOCUS       DASJPH010000166         3690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJPH010000166 DASJPH010000000
VERSION     DASJPH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.170"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..734
                     /locus_tag="U3995_002821"
     CDS             <1..734
                     /locus_tag="U3995_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN1798095.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            846..1742
                     /locus_tag="U3995_002822"
     CDS             846..1742
                     /locus_tag="U3995_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1798096.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1840..2049
                     /locus_tag="U3995_002823"
     CDS             1840..2049
                     /locus_tag="U3995_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1798097.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2067..2339
                     /locus_tag="U3995_002824"
     CDS             2067..2339
                     /locus_tag="U3995_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1798098.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2341..3204
                     /locus_tag="U3995_002825"
     CDS             2341..3204
                     /locus_tag="U3995_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN1798099.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1485 a          562 c          637 g         1006 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagttcaac ttttggggtt cagttcataa agagggcttt tatatattaa
      781 tcacaaatca cttatcacaa atcacaagtg attaatcaca aatcacttgt gatttgtgat
      841 tcttaatgat acaatattac tatacaaaaa aagaatgggg cgtagttatg gagaaggaaa
      901 aactaaaaat acttgaagaa ttaagacgta ttttaaacaa taaaaatgaa gcaattatta
      961 tcttgaataa ttactttaaa ggtggtgttg gaaagtccaa attatcgact atgtttgctt
     1021 acttgacaga caaattgaat ttaaaagttt taatgatcga taaggactta caggcaacat
     1081 tgacaaaaga cttagcaaaa acttttgagg tagaattgcc acgtgtcaat ttttatgaag
     1141 gcttgaaaaa tggaaacttg gcttcttcta ttgttcattt gactgataat ttagacttga
     1201 tccctggcac gtttgatttg atgttactgc caaaattaac tcgctcatgg acttttgaaa
     1261 atgaaagtag attgcttgct actcttttag cacctttaaa aagtgactat gatctcatta
     1321 ttattgatac tgtaccaacg ccaagcgttt atacaaataa tgcaatcgtg gcgagtgatt
     1381 acgttatgat ccctttacaa gcagaagaag aaagtacaaa caacattcaa aactatattt
     1441 cctatttgat tgatttacaa gaacaattta accctggact agatatgatc ggttttgttc
     1501 cttatttagt tgatacggac agcgcaacga taaaatcaaa cctggaagaa ctgtacaagc
     1561 aacataaaga agataacttg gttttccaaa atattatcaa gcgaagtaat aaagtaagta
     1621 cctggtctaa aaatggaatt acagaacaca aaggctatga caaaaaagtt ttatccatgt
     1681 atgagaacgt attttttgaa atgcttgagc gaattattca attagaaaac gaaaaagaat
     1741 agaatcacaa atcacaagtg attaatcaca aatcacttgt gatttgtgat tgttgatgat
     1801 aaaataagaa taagaagaaa tagaaagaag tgagtgattg tgggaaattt aggcgcacaa
     1861 aaagcaaaac gaaatgatac accaatcagt gcaaaaaaag atataatggg agataagacg
     1921 gttcgtgttc gtgctgactt acaccatatt ataaaaattg aaacagcaaa aaatggcgga
     1981 aacgtaaaag aagttatgga tcaagcctta gaagaatata tacggaaata tttacctgat
     2041 aaactttaaa aaggagtgaa aatgaaatgg cagttacgta tgaaaaaaca tttgaaatag
     2101 agatcattaa cgaattatcg gcaagcgttt ataatcgagt attaaactat gttttgaacc
     2161 atgaactaga tactaaaaat actcgtttac tagaagtgaa tcttttaaat caattagaag
     2221 tggcacaaga agttgattta tttcaacaac catttgaaga attacaagct attcatgagt
     2281 attggcggtc aatgaatcaa tattcaaaac aaattttgac aaaagagaaa gtggcttaac
     2341 atggcgaata ttactgactt caccgaaaag caatttgaag atcgtttaga aaagaatgtt
     2401 gaacgactaa ctaaaaatag actagcggtt gaatcgccaa ccgctttttt acttggtggg
     2461 caaccaggat caggtaaaac tagtttgcga tcggcaattt ccgaagagac acaaggaaat
     2521 gttgttatca ttgataatga tacgttcaaa caacagcacc ctaattttga tgaactagtg
     2581 aaactttatg aaaaagacgt agtaaaatac gttacccctt attctaatcg catgacagaa
     2641 gcgatcataa gccgtttgag agataaaggg tataatttag tgattgaagg tacaggacga
     2701 acaacagacg ttcctattca aaccgcaaca atgcttcaag ccaaagatta tgaaacaaaa
     2761 atgtatgtca tggcagtacc taaaatcaac tcatatttag gaacaattga acggtatgaa
     2821 actatgtatg cagatgatcc aatgacagcc agggcaacac caaaacaagc gcatgatatt
     2881 gttgtcaaaa acttaccgac caatttagaa acccttcata aaacgggctt atttagcgat
     2941 ataaggcttt acaatagaga aggagtgaaa ctctattcaa gcttagaaac gccttccatt
     3001 agtccgaaag agaccttaga aagagaatta aatcgtaaag tatcagggaa agaaattcaa
     3061 ccgactttgg agagaataga gcaaaaaatg gttcaaaatc aacaccaaga aacccctgaa
     3121 tttaaagcaa ttcaacaaaa aatggaaagc ttgcagccac ctacaccacc aatacccaaa
     3181 acacctaaac ttccagggat ttaaacctta aaaaagtaaa agagtagtta ccaaaaaacg
     3241 gtaactactc tttttttata aaaacatttc ctcatttttt aaaagctgat cgagaaataa
     3301 agcacacaac tgtattcttt cttttttcag ttggtacatt ttgcagtctg taacgtgcca
     3361 tttactgttt gttaccttaa catatccagt cgaaaacaca agggtatata aacgagcaag
     3421 ctcgcccttg tgttttcttt gtgtctatgt tgcgttctcg tcacagcaaa cgacacgaac
     3481 acactggcga aaatgaacca acaaaacgaa aaaagaaagg agcgtacact gtaatttatt
     3541 tctagctttt aaaacatgag gaaaacagct tcacttcctc aaacgcctaa caaaaatggt
     3601 tgtcaaaaaa ctaaaaatca aaaaaagaaa agaggaaaag aaaatggaaa aaacaaaaaa
     3661 attacaatta gaagatttta cagaaaatgg
//
LOCUS       DASJPH010000167         3602 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJPH010000167 DASJPH010000000
VERSION     DASJPH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.171"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="U3995_002826"
     CDS             <1..186
                     /locus_tag="U3995_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798100.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="U3995_002827"
     CDS             329..553
                     /locus_tag="U3995_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798101.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="U3995_002828"
     CDS             568..1437
                     /locus_tag="U3995_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1798102.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="U3995_002829"
     CDS             1418..2152
                     /locus_tag="U3995_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1798103.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="U3995_002830"
     CDS             2185..3093
                     /locus_tag="U3995_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1798104.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="U3995_002831"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="U3995_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DASJPH010000168         3472 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJPH010000168 DASJPH010000000
VERSION     DASJPH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.172"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U3995_002832"
     CDS             complement(<1..2232)
                     /locus_tag="U3995_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798105.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U3995_002833"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U3995_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          973 a          769 c          549 g         1181 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ct
//
LOCUS       DASJPH010000169         3449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJPH010000169 DASJPH010000000
VERSION     DASJPH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.173"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U3995_002834"
     CDS             complement(150..1337)
                     /locus_tag="U3995_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1798106.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="U3995_002835"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="U3995_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="U3995_002836"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="U3995_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1798107.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="U3995_002837"
     CDS             3305..>3449
                     /locus_tag="U3995_002837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798108.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DASJPH010000170         3399 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJPH010000170 DASJPH010000000
VERSION     DASJPH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.174"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..277
                     /locus_tag="U3995_002838"
     CDS             <1..277
                     /locus_tag="U3995_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEN1798109.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="U3995_002839"
     CDS             420..641
                     /locus_tag="U3995_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798110.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="U3995_002840"
     CDS             657..1499
                     /locus_tag="U3995_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798111.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="U3995_002841"
     CDS             1562..3364
                     /locus_tag="U3995_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN1798112.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          702 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DASJPH010000171         3379 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJPH010000171 DASJPH010000000
VERSION     DASJPH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.175"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..523
                     /locus_tag="U3995_002842"
     CDS             260..523
                     /locus_tag="U3995_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN1798113.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            517..867
                     /locus_tag="U3995_002843"
     CDS             517..867
                     /locus_tag="U3995_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1798114.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(891..1505)
                     /locus_tag="U3995_002844"
     CDS             complement(891..1505)
                     /locus_tag="U3995_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798115.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1819..2241
                     /locus_tag="U3995_002845"
     CDS             1819..2241
                     /locus_tag="U3995_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798116.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2251..2454
                     /locus_tag="U3995_002846"
     CDS             2251..2454
                     /locus_tag="U3995_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798117.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2665..3249
                     /locus_tag="U3995_002847"
     CDS             2665..3249
                     /locus_tag="U3995_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798118.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1165 a          491 c          602 g         1121 t
ORIGIN      
        1 tccattttgt atcaataact atgaaatctt taaagtatcc agaagagatt atttttagat
       61 caattttttt cttacctgat tctaaagatt ttgttttctt aaacatatta tgatttcctt
      121 tcgactattt tcatataaat gttaacatag tttatggatt catggaatgt taatctaatt
      181 taatagaaaa ggataattaa gtatatacgt tttttcttat gtatgttata ctatgtatat
      241 acagaaagag gtgtctacta tggaaatgtt acctataaaa aaatggggaa atagtaatgg
      301 ccttagattg ccaaagtata ttatggaata tcttggaatt catacagaag acaaagtgaa
      361 gataatacaa gaagaagtaa acggtcagaa gcggctaatt atcgaagcgg ctaatataga
      421 aaatgaatta acaattgaac agttatttga aaattacact gatgaaagaa atcatgtgac
      481 gattcaaaat ttaggtgaag cagtaggtaa tgaaaaatgg tgagaaaacc tagacaaggc
      541 gatattctat tattgaatac agcacctcga tctggacacg aacagacagg taaaagacct
      601 tatattgttt taagtcatga tattattgcg gattatagta acgttgttac agttgctcca
      661 atttctagta caactagaaa ctatccgcta tatgtaaata taaactcaga atatgaaatg
      721 aagacaacag gaaaagtatt attagatcaa ctaacgacaa ttgattatga agccagagag
      781 tgtatttttt tggaaaaagc gcatgatagt ttagttgagg agttattgat gaaagttaga
      841 acagtttttc aaaaagtaag caaataaaat agaaacagca gcatgatcaa ctattcgtga
      901 ttgaccatgc tgtctttttt tattctatat acggtttgtc tcgtaacatt gtagtctttt
      961 gcaatcttag cgatcgcaac accttttttt agatcctcaa cgatgttttt ataaacaaga
     1021 cgacgttggg gatcttttgc attggcactg tatagcttag gacgtccttt ataaacaccg
     1081 ttcgcttttg caatcttaat gccttgtgcc tgacgtcgtt tagattcggt acgttcttgt
     1141 tcagcgatca tggccaaaat ttgaatgatc aaatctttaa tgaatttatc caggagagga
     1201 ttgccaattg cttcggccat gatcggtaga cttgtgatga tcagctgcac atttttcttc
     1261 ttcaagtaat tgactgaagc gatgatctcg tcgtaatttc ttccaagtcg atcaatagct
     1321 tccacaataa aaatatcttg atctcggacg aaatccagtg cttcttgaaa aacaagtcga
     1381 tgagtaatcg ttgcgccaga ttgtttctca atgaaaattc tctctgcacc gaattttttc
     1441 atttcttcaa tctgccgttg ttcattttga tcgatggatg agacacgtat gtatgcaact
     1501 ctcaaaattt ttacctactt tctgcaacta tttaaaaaac accctaatca tacacgagaa
     1561 aaccagattt ttcaatgtcg cggtcgtgtc tagttagggt ataccttata tagacaaaaa
     1621 ctaatgatgg aggaaatagt atgtgtcact tttcttttta aatggcaata ttttttgtaa
     1681 ataaatgtca atcaagaaaa tttaaacgat tatgacatta aaaatatatt ttatgaattt
     1741 tctagtatat aacttgattt atctgttaag atttattaag aaaacgtttt cttatttttg
     1801 atttcgaggt gatagacaat gaaaaataag tttattttat ctttaggtat tttaactatt
     1861 ggaattagtg taactggaac tacaattcct acatttgctg atagtacaaa tgaaccaact
     1921 actgtagtag ataataacca agaattaact cctttagata ggaacggaat agccattggt
     1981 aacccaacta acgacttaac tgttactgaa aattatttaa tagtttctcc attacttgct
     2041 cgagcagctt ctaaaacgat acgagtgaca ggaacgatga tttttgataa agttggttgg
     2101 ccttctagac ctcctcaatc acggaattgg aaagaaaaaa gaaatggtta ttggtactat
     2161 ggaaaattgt atttacaaag ttataaatca gctggaaaca attggattgc gacttattca
     2221 ggaaccttaa ctagaaagta gggatttttt atgccagaat ttgtgaacac accaaatttt
     2281 tatcaagata gcgatattct aagtaaaatt aatttaccga aagaagaatc agctttacaa
     2341 gttcagcgaa ctttacagga gacacaaaaa attcaaaaat caatttctaa tctagaagat
     2401 tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc aaaaagaaaa ataatgattt
     2461 taaaaaaaga gtgtctagtc gacattcttt ttttctaatt gaaagtgttc ataaaatcac
     2521 ccatttctgg acactttctt tttttgcttt atttttcaaa aaaacaattc atttcatgtt
     2581 cataaaccca taacaatatc tatgttcagt taaactatat ggataaagtt atgttacaat
     2641 aaaattaatg aaggagtgat ttttttgaaa ataggatacg cccgggtatc tactggttta
     2701 caaaatttgg atttacaaga agacagccta aataaatttg gttgtgaaaa aatttttact
     2761 gatcatatga gtggggcaaa aagtaatcga ccaggattag aaatggccat tgattttgtt
     2821 cgctctggcg atacacttgt cgtttggcgc ttagatcgtt taggtagaaa tatggaagat
     2881 ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga gttttcatag tattcaagaa
     2941 aatattacga tggacaaatc aagttctaca ggacagctca tgtttcattt gtttgcggca
     3001 tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg cagcaggacg agcagctgct
     3061 cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt ctgaacaaga tttggaatta
     3121 ctgaagactt tagtggatag cggtacacct attaagacta ttgcggaacg gtggaacgtc
     3181 tcaagaacca ccatttatcg ttatttggca aaaatggagg agaaaaatgg aaagacaaat
     3241 tctcattagt ttttgtcatt taatgctgaa aatttttgcc atttatctag aaaagtgaca
     3301 atagtatgag aattttttgt aagtgtctac tgataactta ttctcaaatg ttgtaaggat
     3361 tttattcatt tgttaatat
//
LOCUS       DASJPH010000172         3322 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJPH010000172 DASJPH010000000
VERSION     DASJPH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.176"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1357)
                     /locus_tag="U3995_002848"
     CDS             complement(1016..1357)
                     /locus_tag="U3995_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1798119.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="U3995_002849"
     CDS             complement(1357..1587)
                     /locus_tag="U3995_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1798120.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="U3995_002850"
     CDS             1874..2212
                     /locus_tag="U3995_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798121.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2656)
                     /locus_tag="U3995_002851"
     CDS             complement(2417..2656)
                     /locus_tag="U3995_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1798122.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2632..2979)
                     /locus_tag="U3995_002852"
     CDS             complement(2632..2979)
                     /locus_tag="U3995_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1798123.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1072 a          607 c          508 g         1135 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ct
//
LOCUS       DASJPH010000173         3201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJPH010000173 DASJPH010000000
VERSION     DASJPH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.178"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U3995_002853"
     CDS             301..585
                     /locus_tag="U3995_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798124.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U3995_002854"
     CDS             588..1004
                     /locus_tag="U3995_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798125.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U3995_002855"
     CDS             complement(1141..1860)
                     /locus_tag="U3995_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1798126.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2514..2609
                     /locus_tag="U3995_002856"
     CDS             2514..2609
                     /locus_tag="U3995_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1798127.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..3157)
                     /locus_tag="U3995_002857"
     CDS             complement(2912..3157)
                     /locus_tag="U3995_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798128.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DASJPH010000174         3148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJPH010000174 DASJPH010000000
VERSION     DASJPH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.179"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..2874
                     /locus_tag="U3995_002858"
     CDS             205..2874
                     /locus_tag="U3995_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1798129.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT          914 a          587 c          618 g         1029 t
ORIGIN      
        1 ggttttttat ttaataaaaa tttttaaggg tgggactttt gattcctacc ctttttttgc
       61 gtttattttt gttatacgaa caaattatga agttatttat tctaaatttt atttttttgt
      121 gaaaactaga ctctttttaa gcagagaaaa acaaggatat gataaactga atacaacttc
      181 atagaaattt ggtggaaaat aaatatgaaa aaaaatttaa agtcattctt tagagaaaac
      241 ggacgctacg cagcagctag tttcctgatt ccgtttttga ttttagcact tgtttatcta
      301 agtatcggaa tctatcctgg aagcagcaga agtattttgg caagtgatgc cttttcacag
      361 ttttctaatt ttcatgccag cttccgaaat gttcttcttg gaaagcaaag tattttttat
      421 acatggaatg cttctctagg attgaattat ttatctttaa tttcttacta tctaggcggg
      481 atcttcacgc cactggtgtt cttttttcca aaccagatga tgccagatgc actgtatgtt
      541 ctgactttgc ttaaggtcgg ctgtgccgga ttggcttttt ggtttcttgc acgtacgttc
      601 aagattcctc gctgggggca tgttgcactt agcgtcagtt acgcattgat ctcatttatc
      661 actgcacatt cggagatcat catgtggctg gatgcatttt tttatcttcc attagtgatt
      721 ttggggattc atcgtgttat ggatcttaaa cggccgaaac ttttatttat cagttattta
      781 ctcttgtttt tatccagttt ttatatgggt tttatgattg gcgtattttc tttcttatat
      841 tttatcgccc gtctattgac aaactggtca gaatataaaa aaacgattgt tccttatggt
      901 atcacttctt tactggcagg cggcgcttca atgatcattg tattgccagc agtgctggat
      961 cttcggtcaa atggcgaggc actgagtcag atcactacgt tgaaaacaga agcgacttct
     1021 tatctggatc taatcatgaa aaatatgatt ggtgtttatg acacgacaaa atacggatcg
     1081 attccgttcg tttacgcagg tctgctacca ttagtcctct gtctgtttta ttttgccagt
     1141 cggaaagtac cgttgaaaaa taaatttttg tacggcagtc ttttcgccat acttatcgca
     1201 agtttttatc ttgttccatt gaatttgttc tggcatggta tgcatgcacc gaatatgttt
     1261 ttattccgtt atgcctatct gttctctttc ttagtgctcc tgcttgcagg aaaaggatgg
     1321 gaaaagatag aacaagacga tcgtgggctg ttgttaggaa tcattttggt cgtagctgct
     1381 ctctttgcgg tggcatgggg catcaaacct gaaggaagct acacctatgt gacgatgact
     1441 tcctttgtat tgacagtggt tttcttaggg atttatgctt ttgctgtggg attttatcag
     1501 cataaaaaac tttcattcaa tcatctgagt atattgttat tattgatcat gtctgctgaa
     1561 gcactggtca atacgaacag tatgatccat ggcattcttg atgattggaa ttatgcttcc
     1621 cgcagtttat atactgatcc ttatcctgac ttgaaagctc ttgtaaatga aacaaaaaaa
     1681 gattccgact cgttttaccg cttggaaaat ctggatccag tatctgcaaa cgattcgatc
     1741 aactatggtt acagtggtat cagtttgttt tcttctataa gaaatcgtaa ttcttcctct
     1801 tacctggata aattaggttt ccgttcaaga gggacaaatt tgaatatccg ttataacaat
     1861 aatactttgc tgatggatgg gtttacagga atcaaatata acatttctaa aaacgaaagc
     1921 acgttcagta aatatggctt ccgccaagct ggtcaaagcg gcgactatac actgtatcag
     1981 aatgtcaatg cattgccact agcatttact gcaccgttgt ccgttaatga tgttgagcag
     2041 ccagtgacag ataatttgac cagtcagaca aacttgatca atacattgtc tggtctaaat
     2101 gaacagtatt ttacattcta tacacctact ttgaagaatc agttgaatac gacgatcaca
     2161 aagacaacga ctggcgtcac ttactcagaa acagcagata accagccaaa agagctcact
     2221 tgggaagtcc atgtgcctgc aaatacacaa gcttacttaa gtttgttccc aaccaatttt
     2281 gcgcaattag aaagttctag tgcgacgatc actgtcaatg gtacatcgcg aaaaacgcaa
     2341 atcaacattt ctggtcaata ctatgatctt ggctattatc ctcaagagac aacagtgaca
     2401 tttactgcaa gtttttatgg gacgaaagaa ataagtttca tggagcctaa agtcgttggt
     2461 ctagatgttg tagcctatca agccgcaatg aatcagatca aagaaaaagg tgtcgaaatg
     2521 gaagccactg gcagaaaagc ttctgggact gtaaataccg ctgaagattc catgttggtg
     2581 acaactattc cttatgacaa aggatggaaa gctaaaattg atgggaaacc agtcaaggtc
     2641 agcaatttca aagacgcatt cttgatgata aaagttccag caggtaaaca tactgtcact
     2701 ttcagttact tgcctgaagg atttacgata ggtgcaatcc tttttgtcct atgtattgca
     2761 ttgtttattc tttatgtctg gtaccttgac cgcaatcgcc ccttttatgg cacgattgat
     2821 ccagcaatag aagaacaacc acgaaacaca cacagaagaa gacgtagaaa ataaagaaac
     2881 gatggaacct gttcagactg tccgcaccaa gggcaatcaa aacaacgttc catcgttttt
     2941 tggttacaga aaaaataggt aacaaaaaga tgattcactt atcaacgcct cgtccacaag
     3001 tcacgatatt catcaaagat gagaagctgt tttttgaaat tgctctttgt gactcagagt
     3061 taaatgacag gctcacgacc tctttcttaa acggtgttca atcagctgac cttcggtatt
     3121 agctcaacaa acggcaaaat atgaggag
//
LOCUS       DASJPH010000175         3109 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJPH010000175 DASJPH010000000
VERSION     DASJPH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.180"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..1830)
                     /locus_tag="U3995_002859"
     CDS             complement(64..1830)
                     /locus_tag="U3995_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1798130.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1790..>3109)
                     /locus_tag="U3995_002860"
     CDS             complement(1790..>3109)
                     /locus_tag="U3995_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1798131.1"
                     /translation="LSVALGFFINFKIGLVLIAMLVIGVFLLKKMTENTNFIQKYQES
                     LDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPFVLFQW
                     LFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMHLFEAR
                     YAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVLEQKRI
                     YALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLF
                     NQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSGGEKQR
                     IAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSSIKSVD
                     EILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT         1019 a          582 c          438 g         1070 t
ORIGIN      
        1 tctttttgaa aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt
       61 tcatcatcgt gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt
      121 tatgagattt ttgtatattt ctgaccttcg atacaactca ttatgagtac cttgatcaac
      181 tagttttcct tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg
      241 cgatataatt agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt
      301 ttcattttcc gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt
      361 caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt
      421 ttctccgacc aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc
      481 taaacgagct gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt
      541 ttccaaaata cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa
      601 caaagaagct ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc
      661 ataatcatac agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc
      721 tactaacgca gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt
      781 attgttatca tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact
      841 atctgatccc tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc
      901 taaataaaat atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat
      961 tgcggctagt aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg
     1021 aacacctact agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat
     1081 gactgaaaaa gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt
     1141 gaaaagtctg tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc
     1201 tatagtttct tgaaattctt ccgagttttc tctcaaagta tcgtaatatt tagtattggc
     1261 tttcgtttgg gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat
     1321 aacagctaat cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa
     1381 cagagccata ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac
     1441 gtcagacatg atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg
     1501 taaatttgac aaatcgtctg caatttgcac tcttaaatgc gcactttctt tataagtact
     1561 attatataag ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacaaa
     1621 cgatacggca aaataaagcc aacggggctt tgtattttct aaaactaatt cgtctatcag
     1681 catcattata atgaatgctg gtaccatatt aatgcagtat acaaaaaaac tagaaataga
     1741 agctttagtt aaagcttctg cgccctgatc acttaaagca aatttgtttt tataaaaact
     1801 tttcattgtc aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca
     1861 cttgatgttt agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca
     1921 agacaagaat ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg
     1981 ttttttcttt catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa
     2041 tagatgcact ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa
     2101 tagctatacg ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat
     2161 gctctctttc gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa
     2221 atactttact tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat
     2281 tgaatagttt cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat
     2341 agtgatttaa aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg
     2401 aaattagttt agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc
     2461 ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc
     2521 tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt
     2581 ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa
     2641 acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa
     2701 aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa
     2761 aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa
     2821 aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt
     2881 tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact tgaattccgc
     2941 ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa
     3001 aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta
     3061 gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataa
//
LOCUS       DASJPH010000176         3071 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJPH010000176 DASJPH010000000
VERSION     DASJPH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.181"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..1102
                     /locus_tag="U3995_002861"
     CDS             287..1102
                     /locus_tag="U3995_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798132.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="U3995_002862"
     CDS             1228..1485
                     /locus_tag="U3995_002862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798133.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="U3995_002863"
     CDS             complement(1737..2309)
                     /locus_tag="U3995_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798134.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /locus_tag="U3995_002864"
     CDS             complement(2325..2930)
                     /locus_tag="U3995_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN1798135.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DASJPH010000177         3019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJPH010000177 DASJPH010000000
VERSION     DASJPH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.182"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(106..1053)
                     /locus_tag="U3995_002865"
     CDS             complement(106..1053)
                     /locus_tag="U3995_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1798136.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="U3995_002866"
     CDS             1299..1763
                     /locus_tag="U3995_002866"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1798137.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="U3995_002867"
     CDS             complement(1825..2868)
                     /locus_tag="U3995_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1798138.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          997 a          599 c          578 g          845 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacg
//
LOCUS       DASJPH010000178         2960 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJPH010000178 DASJPH010000000
VERSION     DASJPH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.184"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="U3995_002868"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="U3995_002868"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1798139.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="U3995_002869"
     CDS             complement(1034..1954)
                     /locus_tag="U3995_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1798140.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="U3995_002870"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="U3995_002870"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1798141.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          831 a          596 c          513 g         1020 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt
//
LOCUS       DASJPH010000179         2821 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJPH010000179 DASJPH010000000
VERSION     DASJPH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.185"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            134..307
                     /locus_tag="U3995_002871"
     CDS             134..307
                     /locus_tag="U3995_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798142.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="U3995_002872"
     CDS             947..1420
                     /locus_tag="U3995_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN1798143.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="U3995_002873"
     CDS             1429..1656
                     /locus_tag="U3995_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798144.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2292..2663
                     /locus_tag="U3995_002874"
     CDS             2292..2663
                     /locus_tag="U3995_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798145.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          904 a          456 c          417 g         1044 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
     2221 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
     2281 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
     2341 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
     2401 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
     2461 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
     2521 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
     2581 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
     2641 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
     2701 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
     2761 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat
     2821 g
//
LOCUS       DASJPH010000180         2736 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJPH010000180 DASJPH010000000
VERSION     DASJPH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.186"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..470
                     /locus_tag="U3995_002875"
     CDS             27..470
                     /locus_tag="U3995_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798146.1"
                     /translation="MVVEDYDKVDGRNALNTDAKGLSLGLAQWNDRGKVDISGKVWRH
                     TGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENPVVDIIGLQGNRM
                     TVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            503..2731
                     /locus_tag="U3995_002876"
     CDS             503..2731
                     /locus_tag="U3995_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1798147.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT         1008 a          496 c          584 g          648 t
ORIGIN      
        1 caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa
       61 gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc
      121 gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac
      181 agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa
      241 gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg
      301 tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa
      361 agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg
      421 gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga
      481 tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc
      541 acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga
      601 atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa
      661 cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc
      721 tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt
      781 tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag aattactaga
      841 agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg
      901 cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga
      961 ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa
     1021 aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc
     1081 acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg
     1141 ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt
     1201 aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga
     1261 agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa
     1321 gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt
     1381 agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact
     1441 ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat
     1501 tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa
     1561 agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga
     1621 agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa
     1681 atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa
     1741 aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca
     1801 aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca
     1861 tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat
     1921 tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc
     1981 acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga
     2041 gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt
     2101 caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca
     2161 tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga
     2221 tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt
     2281 cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc
     2341 tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac
     2401 tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt
     2461 aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc
     2521 gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata
     2581 tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa
     2641 gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca
     2701 aatgattcaa ctgattgccc gctatctatg ataaaa
//
LOCUS       DASJPH010000181         2734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJPH010000181 DASJPH010000000
VERSION     DASJPH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.187"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..521
                     /locus_tag="U3995_002877"
     CDS             75..521
                     /locus_tag="U3995_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1798148.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            794..2146
                     /locus_tag="U3995_002878"
     CDS             794..2146
                     /locus_tag="U3995_002878"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1798149.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2317..2604
                     /locus_tag="U3995_002879"
     CDS             2317..2604
                     /locus_tag="U3995_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798150.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          960 a          449 c          544 g          781 t
ORIGIN      
        1 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
       61 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
      121 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
      181 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
      241 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
      301 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
      361 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
      421 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
      481 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
      541 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
      601 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
      661 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
      721 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
      781 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
      841 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
      901 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
      961 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     1021 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     1081 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     1141 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     1201 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     1261 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     1321 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     1381 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     1441 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     1501 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     1561 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     1621 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     1681 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     1741 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     1801 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     1861 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     1921 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     1981 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     2041 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     2101 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     2161 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     2221 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     2281 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     2341 caagatgcat ttttagaaga ttataaaagt gcttcgaaga aattaacgat caatattgat
     2401 gagttggcag atcaagtact ctctcagttg aatgaaacgc aagaggaata ttacacgatg
     2461 ccagcaaatg acgccaaaga taatgtttct catcgttttc ctttcagtag acgcatctac
     2521 tcagatgaaa atgacggcac attgaataca gagtacacat atgaaggtca gccatatgtg
     2581 ctagaaactg atgatgatct ttaaagacag tttttgaaaa ggccgctcat ccttaagtga
     2641 tgacaaaaaa tcatcacaat taccgatgac tgtctcttta ctatctttta atcagaaaaa
     2701 ggggttatga caagctttgt caaccccttt tttt
//
LOCUS       DASJPH010000182         2733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJPH010000182 DASJPH010000000
VERSION     DASJPH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.188"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..709
                     /locus_tag="U3995_002880"
     CDS             <1..709
                     /locus_tag="U3995_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1798151.1"
                     /translation="PFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLS
                     TGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDH
                     SLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGD
                     GVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKK
                     VKRPISADSVLLEEDVE"
     gene            706..1854
                     /gene="neuC"
                     /locus_tag="U3995_002881"
     CDS             706..1854
                     /gene="neuC"
                     /locus_tag="U3995_002881"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN1798152.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1827..2504
                     /locus_tag="U3995_002882"
     CDS             1827..2504
                     /locus_tag="U3995_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1798153.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2455..>2733
                     /locus_tag="U3995_002883"
     CDS             2455..>2733
                     /locus_tag="U3995_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEN1798154.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFA"
BASE COUNT          995 a          350 c          551 g          837 t
ORIGIN      
        1 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
       61 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
      121 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
      181 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
      241 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
      301 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
      361 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
      421 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
      481 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
      541 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
      601 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
      661 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
      721 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
      781 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
      841 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
      901 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
      961 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
     1021 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
     1081 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
     1141 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
     1201 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
     1261 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
     1321 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
     1381 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
     1441 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
     1501 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
     1561 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
     1621 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
     1681 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
     1741 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
     1801 atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta ttaaaatata
     1861 ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt attgaaacct
     1921 taagattaga aaataagtta atatatggat tttttgatga taagcaaaat aaaatgcaag
     1981 atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa
     2041 tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat
     2101 tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag
     2161 aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg
     2221 taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta
     2281 ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag
     2341 gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta
     2401 tgataggcgc agggagcact attgttaagg atattccctc acattcctta gcttatggca
     2461 atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt
     2521 cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata
     2581 gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct
     2641 aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg
     2701 cttaatgttg gaaaaaacga tattgtcttt gct
//
LOCUS       DASJPH010000183         2468 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJPH010000183 DASJPH010000000
VERSION     DASJPH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.190"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(337..636)
                     /locus_tag="U3995_002884"
     CDS             complement(337..636)
                     /locus_tag="U3995_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1798155.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            987..1619
                     /locus_tag="U3995_002885"
     CDS             987..1619
                     /locus_tag="U3995_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1798156.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1742..2380
                     /locus_tag="U3995_002886"
     CDS             1742..2380
                     /locus_tag="U3995_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1798157.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          824 a          386 c          345 g          913 t
ORIGIN      
        1 gtttttacaa ggtttttcct aatgtaaatt attttttcgc atatattcat tttattttac
       61 ttattgcttt cattttttca atctatattt ccaattttta acttacaaaa atgcaacatt
      121 aataaataac gatagtttca taaaatacac ttttaattaa cattattagt ttatactagc
      181 tgtttttgtg ttaattaaag gataaaatac aatataaaga aacatctgtt tctttattta
      241 gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac
      301 tatacttttt aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg
      361 tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat
      421 ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg
      481 ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc
      541 tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat catataattt
      601 tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat
      661 ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt
      721 actggattgt taaaaccttt caatacactg ttatggcaac gatttttatt ttaaaaacat
      781 tctaaattaa atcagtaaaa atttttattc taagattttt attagaatta cgcctaattt
      841 gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc gttatcgaaa
      901 aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt gttaatatga
      961 ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa gcaagcttga
     1021 ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat aatatccttt
     1081 cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac aaatctattt
     1141 tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc gactgtatat
     1201 tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat cgcgtgaaat
     1261 ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg aacacgttat
     1321 tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa gaaagtacct
     1381 ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact aaaataatgt
     1441 tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa aatatcgatt
     1501 taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa aattgtacgc
     1561 ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa gtagtataat
     1621 caaaatgaat atataagata aatttttagc aatacaaata tccataaaaa aacattgcct
     1681 attttaactc ttcatgtata ctgactctat cttcaaaaac tgaatagtag gagttattat
     1741 catgaataac attttaggaa aaaaaataga acttaaaaaa gggatattgg gcactattgt
     1801 cgttgtactc ggtttgttag ggttgctatt tgcgccatcc attattagca ttttttcttt
     1861 aactatcact ggatttatag catttacact tgtttttggt aaaaaagata cgaaaaaaat
     1921 gttctcgaaa cctgtcgctc ctgtcaaaaa tatagccaaa tattttttta tcaatgtagt
     1981 aatcagtgcc gcagtctcgg tatttttgca acaaattttg aaatggggtt taacaggcaa
     2041 tccaataaat gaggctttca gcccgttatt atttattatt ttgcctatca tgatacttgg
     2101 agaagaattg ttctcagtgt attttctcgc tattttttct agtaagttca gcattcctgt
     2161 cgctagtttc ttgtcagcaa tcatctttgg atttatccat tacagtactt atgataatgg
     2221 aaatatttta cacactgtag cacacatcct tttgatacaa ggggtggcaa gattgttatt
     2281 caaccaagct gccattaaaa gcaactcgat cattacaagt tgggcagttc atgtgatatt
     2341 tgatttcaca gctatattgc tagtcgtttt attctcctaa atttttatag aaataataca
     2401 tgctctattg ctaagaaagc atactttatt tcctttaaac aaaaagacag atgtatcaat
     2461 ggattaaa
//
LOCUS       DASJPH010000184         2430 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJPH010000184 DASJPH010000000
VERSION     DASJPH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.191"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..935
                     /locus_tag="U3995_002887"
     CDS             <1..935
                     /locus_tag="U3995_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1798158.1"
                     /translation="ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVD
                     VSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASE
                     GIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNK
                     PQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIV
                     TATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPK
                     TGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1069..2412
                     /locus_tag="U3995_002888"
     CDS             1069..2412
                     /locus_tag="U3995_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1798159.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          964 a          308 c          455 g          703 t
ORIGIN      
        1 atgcagataa atacaatcct gaaggacaaa aagtgactac agacttaaat aaagagccag
       61 atgcatctga gggaattaag aacaaagaag atttaccaaa aggaactact tatgcttgga
      121 aagagaaagt agatgttagc acagctggaa ataaaaaagg tactattgta gtgacatatc
      181 cagatggatc atctgatgaa gttgaagtag acgttacagt gacagatact cgttccgatg
      241 cagataaata taatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      301 catctgaggg aattaagaac aaagaagatc taccaaaagg aactacttat acttggaaag
      361 aaaaagtaga tgttagtaca gctggaaaca aaaaaggtac tgtagtagta acatatccag
      421 atggatctaa agaagaagta gaagttacta tttctgtagt agataaaaaa gcaccgaata
      481 aacctcaagt tgatcctatt acagagggtg accaaattgt tactggtaaa actgaaccaa
      541 atgcagaggt gacagttaca ttaccagacg gaagtcaaca ccatgggaca gctgataaaa
      601 atggtaattt tacagttaaa gttcctaaat tagaggcagg gacaaaagtg atagtaactg
      661 caactgatga atctggtaat actagtgaac ctactaatgt agttgtttct tccaacaaaa
      721 aagacagtgg aaaaaacggt agtaaaggta gtaagactga caatcaaggt agcaacagca
      781 atcaagacaa aaatagaggc aaaagccagt cctcaaaagt ttttcctaag acgggagaga
      841 gtgacagtaa tatctttact attagcggtg gtttaatact gttaggaacg ttaggattat
      901 tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta agtcttctct
      961 gagaagagga agaatctttt tcttcctctt tttgcttttt tatgatggga aataagtgtt
     1021 agttaaagat gaatatactt tttatagaaa ggaaaagaat gtaattaagt ggaaaaaata
     1081 gtaattattg gttcaaacca ttcagggcta gccgcagctc gatttctaaa cttaagcgat
     1141 tcaaattatg aagttattct aatcgataaa aaatcagaaa ttagttactt ggaatgtggg
     1201 acatcgttat ttataaaagg agaagtaagt aatttagaag atctttttta tcaaaataga
     1261 atgacactaa attctgagat aaaaaagtta tttctagaga cagaagttga caaaattgat
     1321 attcaaaata aaaagattag ttgtgtaaaa aaagatggta caagattctc tcaagaatat
     1381 gacaaagtta tattagctac aggttcaaag caaattatgc tagatatacc ggggaataat
     1441 ttagctaata ttttttcgat aaagaatttt ccaaatgcaa aagagatttt taaagctttg
     1501 gatgatagta ctattaaaaa agtaggaatt attggagcag gatatattgg agttgaaatt
     1561 gctgaagcca taagaaaaag agggaaagaa gtatatcttt tcgatgttgc agatagagtg
     1621 ttgtcgactt attacgaccg tagtttttca gataaagttg aagaaatact atcaaaaaat
     1681 ggaatacatc tttgtctatc agaaagtcct aaaaaatatt taggaagaaa aaagattgaa
     1741 aaaatagtca ctacaagtgg tacccaatac tctatggata tggtcgtaca agctatagga
     1801 tttctatcta atagtccaat tggggaaaag gaactcttga gagatgttag tggagcatat
     1861 ctaactaatg aataccagca aacaaatata aaagatatat atgctattgg ggattgtgct
     1921 acaacttttt ttacgtccat taatagaagt agtgttgatt tttctatttc taatgctcta
     1981 agaacagctt atattgcaac tcaacatatt aacaatcaag attttacacc ttctggttcc
     2041 caatttacaa atggcttttc tatatttaat tacaattttt acgcagcagg tttgaattta
     2101 aaaacagcac gattattaag tgagtctata gattatatag aaatagaaga tttcattaga
     2161 cctgcttttt tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga aaaaaataca
     2221 aagagaatag taggtgtaca actttgtagt caagaagatt tatctggact attatcaatg
     2281 ttttctcttg ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta
     2341 tttcatccta gttttagcca accctataat tttattttaa aggcagtaac aggattaaac
     2401 attgaaacat aatcgttact aaatgaaatc
//
LOCUS       DASJPH010000185         2423 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJPH010000185 DASJPH010000000
VERSION     DASJPH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.192"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(4..249)
                     /locus_tag="U3995_002889"
     CDS             complement(4..249)
                     /locus_tag="U3995_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798160.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="U3995_002890"
     CDS             complement(266..1117)
                     /locus_tag="U3995_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1798161.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="U3995_002891"
     CDS             1283..2287
                     /locus_tag="U3995_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1798162.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          776 a          411 c          487 g          749 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctcacgct cta
//
LOCUS       DASJPH010000186         2421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJPH010000186 DASJPH010000000
VERSION     DASJPH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.193"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1571)
                     /locus_tag="U3995_002892"
     CDS             complement(<1..1571)
                     /locus_tag="U3995_002892"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1798163.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKA"
BASE COUNT          723 a          514 c          395 g          789 t
ORIGIN      
        1 gctttgatta atttttgcgg gcgatttgga cgagaaatca attctgggtg gaattggcat
       61 cccacaaaga attttttctc tggcagctct acgatttcta ctaaacggtt atctggtgag
      121 acacctgaaa agactagccc ttgttcttca aacaattgac gatatttgtt attgaactca
      181 tagcggtgac ggtgacgttc ttgcacaaca tcggctccat catatgcttc agccgtcttc
      241 gttccttttt tgattttaca tgggtataat ccaaggcgta aagttccacc caaattctcg
      301 atgttttcct gatcagccat taaatcaata atattgtttg ctgtatctgg attcgtttca
      361 gctgatgacg catcttctaa tccaacgacg ttacgtgcaa attcgacaca agccatctgc
      421 ataccaagac aaatacctaa aaacggaacg tcattttcgc gagcataacg aatcgcttcg
      481 attttccctt ctactccgcg atcaccaaat ccaccaggaa ccaagattcc atctgcgtct
      541 ttcaaacgtt cttctacatt ttctctcgtt aattcttgtg ctttgatcca atcgatctcg
      601 atgtctgaat tataggcgaa accagaatgt ttcaatgctt caactactga gatatatgca
      661 tctggtaact cgacatattt accgacaagc gcaatccgta cttttttctt caagttcaag
      721 acacgttctt ctaattcttt ccattctgtc atgtctgctt ctggtgtatc tagttttaaa
      781 tggtcgcaaa caatttgatc cattccttgt gcttgtaaaa ctaatgggat ggagtaaagt
      841 gtatctacat ctggcgactc gataactgct tctggttcga catcacaaaa ttgtgccaat
      901 ttgtttttca cgctttgaga aacaggttgc tctgtacgta ccaccaaaat attcggttgg
      961 atacctaaac cacgtagctc ttttacactg tgctgcgttg gtttagtttt catttctcca
     1021 gcagctttca agtaaggaat caatgttgta tggatataca taacattatc tgtaccgaca
     1081 tctgctttca tttgacgaag tgcttcaagg aatggcaaag attctatatc ccctactgtt
     1141 ccccctactt ctgtaatgat cacatctgaa tcagtcattt tagctgctcg catgattttt
     1201 tctttgatct catttgtaat atgaggaata acttgaacag ttgctcccaa gtattcgccc
     1261 ttacgttctt tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt ggagtatttg
     1321 ttcaaattga tgtcaataaa acgttcgtag tggcccagat ccaaatctgt ttcagcacca
     1381 tcatctgtta cgaatacttc tccgtgctga tatgggctca tcgtccccgg atccacgttg
     1441 atgtatggat caaatttttg gatagttact tttaagccgc ggtttttcaa taagcgtcct
     1501 aaagaagctg caacaatccc ttttccgatt gatgaaacga caccgccggt cacaaaaata
     1561 tattttgtca taagttcaaa aactcctttt taatttatgt gcaggaaaag tctcagacaa
     1621 taaacaaagc tccctattct tcaataagaa tagggagccg aaatttctgt aaacttctga
     1681 ccctctaaaa gggtgcccaa ggtgtataat acaagctgac taagggaaag tcaagtactc
     1741 aaatgaatac tatccgctta caattttttc tttttcgata tcctttgaga aattatttct
     1801 taagtccttt atgagtgcaa aaaaaattta acccacataa taagcttgat ataaacatag
     1861 ctaacgttaa acctaagcaa atcagaaaat cgttactgaa aaattttaag tgaaataata
     1921 gataaaaaaa taaaacaaac attatgatag ataatatccc acctaacaaa gaactacttt
     1981 ttcctacttg cagcaacagt ttatatagct tatcgtagaa acaatctttt tctttcaccc
     2041 ttatttacct cctttacgta aaaaacgtag tccacagttc tcggattttt ggcatatctt
     2101 ttctattaaa taatatcaaa aacaaaacaa ttccactaat tttttcaaag atgacctttt
     2161 tttcaacaaa agtgccttgt agggattcat tgaaggtaac gaatcacata agtaaaaaat
     2221 aaggagatgc gacaaaagtc gtgcctcctt atttctaaat aaacggtgtt ctcctgccaa
     2281 ctcctcggtc acaagtcaca atatccatca aacatgagaa gctgtttttg aaattatttc
     2341 ttgtgattcg gagttaaatg gtaggctcac aacctctgga taaacggtat tcaaaagcca
     2401 gtttctcggt attaagccaa a
//
LOCUS       DASJPH010000187         2418 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJPH010000187 DASJPH010000000
VERSION     DASJPH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.194"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1149
                     /locus_tag="U3995_002893"
                     /pseudo
     CDS             5..1149
                     /locus_tag="U3995_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="U3995_002894"
     CDS             1320..1778
                     /locus_tag="U3995_002894"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1798164.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="U3995_002895"
     CDS             1937..2407
                     /locus_tag="U3995_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1798165.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DASJPH010000188         2414 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJPH010000188 DASJPH010000000
VERSION     DASJPH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.195"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..145
                     /locus_tag="U3995_002896"
     CDS             <1..145
                     /locus_tag="U3995_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798166.1"
                     /translation="DFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS
                     SLI"
     gene            complement(1011..1541)
                     /locus_tag="U3995_002897"
     CDS             complement(1011..1541)
                     /locus_tag="U3995_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN1798167.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1538..2155)
                     /locus_tag="U3995_002898"
     CDS             complement(1538..2155)
                     /locus_tag="U3995_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1798168.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          797 a          458 c          264 g          895 t
ORIGIN      
        1 agattttaga gaagtgaatc agacatttat ttccagtgtc agcaatcaac gcaatcatat
       61 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
      121 agcattttat tctagcttaa tttgacaaat catataattt aaaattaaca ttgattctcc
      181 tatgaaaata tcttttacct ttctcctgtg aaaaggaaat ttcctttttt atagtgttat
      241 tgatataatc agaatattca tcaaactctg tatctggagt taaaaatcta cagtaacatc
      301 tataagcttt gctagtaatc ttcttgggtt ctctctccat tgtcaagcaa catcaaaaca
      361 cctacttact attcgattct tttctgccaa aaacgagtca gcatcactca caacttatgt
      421 gttgctgact ccgactagtc ctttgttcaa tatcaactta ttaaacagat ataatatttt
      481 tctttatttc gtatactcca agacttccat ctttctttca tcttatctct gaaaaaaaca
      541 cagcaaagtg cccttaataa atatacatta gaatttgcaa attttttcat cttaagatat
      601 atcctattca tattctttac aatcctaatg ggtgattaca aatctattta gttcaagttc
      661 tacattataa acacctttct tgatagtatc atctgctgca caagcgatgt cgatccactt
      721 attaggggat ttacgtgctc aaaagtggct attccttttg ggaagtccat ccataataat
      781 ggtccatgga tttttttagg gtcttgtcgt tcaaatttct caaaaacttt attatacttc
      841 catagtttta taattatttg gtttcacttt ggctcgttca aacaccaact tgtatttctt
      901 ttcaacgttc gaactggagc cccccctata atctcattat tattttcatg actttttact
      961 accgtggaat tacaagcaac aacaatatta tctgctaaat gaacaccttt ctatatcacg
     1021 ctttttgctc caatccaaca atgtttacct ataactattt tattattttt attcaaaatc
     1081 ctatgagtat tcaattcaaa aatcgtatgt gaatcggtat ccataaataa tgtatcccac
     1141 gatactgtac agccatcttt gaattgaatt tccttaaagc aaattatact catttttcct
     1201 gtcgaattaa actttgaccc aatatctaaa actgcatttt tccttatgtc aatatgagcc
     1261 ccttgtgcga taaacgctgg cccttgtatt cttagtattc cttcaatact tataatcgtt
     1321 tcttcatttc ccgcagtatt taatactgga aagcccaaat aaactatttt tccagattgt
     1381 aatactatct caattttccc tctcgatttg cgtgtaatcc gtactttacg atgcaccaaa
     1441 ataggtaatc tccacgcatc tgaaatatga agaaatttat aattgaagta tatcgtttta
     1501 ggtaaattta atatattctt tattatcaat ccgagtctca tctttcacca tctctttttg
     1561 taatcaaaat tagtttataa aggattacac ccaatatacc gcccaacgta ttatatacaa
     1621 aatccgaaat ttgaaacgtt cctagcctca aaaaaagttg taaaaattca attattgctg
     1681 aaaaataaaa tgcaattttt cctccatata ctattgagga ccatattttg ggtttcttta
     1741 tattttcata tttaatagac attaccatat aaacatatgg aataaaaaga ataatatttt
     1801 ctaaacacgc tgtagataac tcttttgttc cagttgttgt attatactta taaatccacc
     1861 agtctcccat aacatcagat aaaggattta accaaattgt tcgattgatt aatgttgtaa
     1921 ataaaatcat cgcagtatag aatatcaaag cacaaacttt tctatatttt ctatccttct
     1981 ttaaatgaag ataaagttcc attagtatac gtttatagcc ctcacctgca gcaatgccat
     2041 ctgttgcata cataaaatag accagtccta aaattgtcaa taaaacagca aacccaaagg
     2101 gttcatataa tttatataat atatctgtaa taatttctgc aatcactatt atcataatta
     2161 tttctccaat atcaagtata cacttatcgc ttccaattac acatgatcat attagagtgt
     2221 attatcacat tctctaatat tcaatttatt tttcctaaaa tattcgaaat aatcgtcttg
     2281 ataacgagta agtattttta tacgattgat aacatagtaa caatattttt aattatagta
     2341 aaaaatcatc tgcctttttc atccacctaa tcttattttt cattttattc aacatcctta
     2401 aataaacttc cgtt
//
LOCUS       DASJPH010000189         2398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJPH010000189 DASJPH010000000
VERSION     DASJPH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.196"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U3995_002899"
     CDS             207..2069
                     /locus_tag="U3995_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1798169.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U3995_002900"
     CDS             2069..>2398
                     /locus_tag="U3995_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1798170.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASJPH010000190         2300 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJPH010000190 DASJPH010000000
VERSION     DASJPH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.197"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1364)
                     /locus_tag="U3995_002901"
     CDS             complement(45..1364)
                     /locus_tag="U3995_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1798171.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="U3995_002902"
     CDS             complement(1361..2014)
                     /locus_tag="U3995_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1798172.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          760 a          359 c          195 g          986 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc
//
LOCUS       DASJPH010000191         2291 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJPH010000191 DASJPH010000000
VERSION     DASJPH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.199"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(96..1343)
                     /locus_tag="U3995_002903"
     CDS             complement(96..1343)
                     /locus_tag="U3995_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1798173.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1419..1565)
                     /locus_tag="U3995_002904"
     CDS             complement(1419..1565)
                     /locus_tag="U3995_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1798174.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1669..1980)
                     /locus_tag="U3995_002905"
     CDS             complement(1669..1980)
                     /locus_tag="U3995_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1798175.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1982..2179)
                     /locus_tag="U3995_002906"
     CDS             complement(1982..2179)
                     /locus_tag="U3995_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1798176.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          812 a          379 c          245 g          855 t
ORIGIN      
        1 aagaatgaaa ttatcaataa ttcgttctaa ctgatttaat tgtataattt gatcttcaca
       61 taatattatt ggataactca taaatcctcc ttccctcaaa acaacaaaat aacttgagta
      121 gtaaattgat acggatcgtt ttgatagttc acaaacatat tgggattctt tttattgatt
      181 tcctctatac tagataaacc taacccttga tgtccctttt ttgttgttat tccttttttc
      241 attagagtat ctatagagag tagtatctct ctacaactat tcgatatcga aatttctatc
      301 tgtttatcat ctcggtaaat agctagagac agttctctat cttgactctc tatagatgct
      361 tcaattgcat tatcgagaat aattccaagc accctaatac agtcaaatat tggaacagga
      421 acttcaataa tagtacttgg acattcgaat ttacaacaaa tcttattgtt atttgcttgg
      481 tatagcttcg ctatcaacaa acttttcaaa tattcatttt ttatattttt taaataacga
      541 tattcaaact tattggttag ataagaatta gaatagatct ctaattcttt tatttctttt
      601 tccaatatcg aatcatggtt ttgattagcg atttctttta aactcagcaa taaattttta
      661 tagtcatgtc taaatctggc aaatttttct tgatcttttt ccagtgaatc agtatatttt
      721 tttagataaa ccaattcttt tcttttcaat tgctgttcat attgttctcg ttgcctaata
      781 gttattctaa taaataaaaa tagaacaaca aacatttgaa tgattaaaaa tattaagaca
      841 ccaaacacaa aatgatcaaa tacttgatat tgatgagcaa cataagaaac aaataaacca
      901 attgtaagta aataaatcat gaatatagca gtaagtttag aactggactg ttcaacaatc
      961 ctatttatag ctaattttct atataaccaa ataaacatca gcaaaaacaa agaatcaagc
     1021 attatctgga atactacata accataccct tttatccctt caaatgaaaa catatacgtc
     1081 ataaccatgg aagtaataat agatctgaaa tcatcaataa gcatacataa tagtaattta
     1141 tttaaaagca ctatattagt tttcttcttt tcctttaata taaaataact tccgattata
     1201 ggtatagctg cggaaacatc agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa
     1261 catacaaaaa gatattttct tttgttaaga tcagtattag tcatataaaa taagagaaaa
     1321 aagaaaaagt ttcccacgaa cattcttaat agataagctt ttgtatcgat agtaatcaag
     1381 agatcccctc ctgattttag gatatcataa aaaaaatttt agttacactt tttaaataat
     1441 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
     1501 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
     1561 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
     1621 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
     1681 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
     1741 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
     1801 aaaatataga taattcacca atctatttat taaaggaccg ggatctaatt catatttttg
     1861 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
     1921 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
     1981 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
     2041 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
     2101 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
     2161 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
     2221 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
     2281 ataagaattt a
//
LOCUS       DASJPH010000192         2238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJPH010000192 DASJPH010000000
VERSION     DASJPH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.200"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..445
                     /locus_tag="U3995_002907"
     CDS             191..445
                     /locus_tag="U3995_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1798177.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="U3995_002908"
     CDS             446..763
                     /locus_tag="U3995_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1798178.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>2238
                     /locus_tag="U3995_002909"
     CDS             1322..>2238
                     /locus_tag="U3995_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798179.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKG"
BASE COUNT          816 a          301 c          422 g          699 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaagg
//
LOCUS       DASJPH010000193         2227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJPH010000193 DASJPH010000000
VERSION     DASJPH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.201"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="U3995_002910"
     CDS             complement(<1..280)
                     /locus_tag="U3995_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1798180.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="U3995_002911"
                     /pseudo
     CDS             742..2124
                     /locus_tag="U3995_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DASJPH010000194         2201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJPH010000194 DASJPH010000000
VERSION     DASJPH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.202"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..1090)
                     /gene="cysK"
                     /locus_tag="U3995_002912"
     CDS             complement(164..1090)
                     /gene="cysK"
                     /locus_tag="U3995_002912"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1798181.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1230..2117)
                     /locus_tag="U3995_002913"
     CDS             complement(1230..2117)
                     /locus_tag="U3995_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1798182.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          615 a          486 c          365 g          735 t
ORIGIN      
        1 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
       61 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
      121 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
      181 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
      241 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
      301 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
      361 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
      421 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
      481 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
      541 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
      601 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
      661 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
      721 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
      781 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
      841 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
      901 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
      961 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     1021 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     1081 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     1141 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     1201 atggtaattt ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa
     1261 gaataactgg acatagacat gccatttttc gccattagat catcaacaga catgccaaat
     1321 ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt
     1381 gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt
     1441 gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa
     1501 gtattaatca atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc
     1561 aagtacgctg ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga
     1621 taatcgcccg tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc
     1681 caataagaag aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat
     1741 tctttggttg ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta
     1801 taatttggcg cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg
     1861 atcatgactg aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt
     1921 tgatcgataa acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca
     1981 tgttgcggta ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga
     2041 gcaatcgctt ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg
     2101 gtcttcagct cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc
     2161 ttcttaaaac aaacttaatt aattaaatta tacttttaat t
//
LOCUS       DASJPH010000195         2151 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJPH010000195 DASJPH010000000
VERSION     DASJPH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.203"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..861)
                     /locus_tag="U3995_002914"
     CDS             complement(<1..861)
                     /locus_tag="U3995_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1798183.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFH"
     gene            complement(1070..1939)
                     /locus_tag="U3995_002915"
     CDS             complement(1070..1939)
                     /locus_tag="U3995_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN1798184.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
BASE COUNT          610 a          448 c          348 g          745 t
ORIGIN      
        1 atgaaaaaga atcgcatgac cgtcttctac atcctttttt aagtcccatc ctaaagaacg
       61 atagccattt tttttcggtg tttgatcttg cagtaaagaa tcaatcgtct cttcttctaa
      121 aaaacgacca ttacggattt ttcccttgtt caaatacatt tggacaaaag taatgctgtc
      181 tttcaaatca ataaagagac ccgcgttacc tgcatgttca gccaaaactc ttgctttcgg
      241 atcatgagtt tgtccacgca aaacctcacc cgaaggcagt tgttctgtgg gaacgatttt
      301 tttggaagga aaaggaagaa ataagctgtt catcattcct aatggttcca atacttgttc
      361 ttgaaagatt tccactgcag tttcttggta aatttcctct agcatcaacc caagaagaat
      421 cgtaccggca tctgtatatt ttacttcttt tcctagttgc tcacctggtt ctagcttcaa
      481 ataagcggcg atcaattctt cctgagatag cttatctctg tttggtatcc atgttttgat
      541 atcagaagta tgtgtcagta aatgacgcaa agtgattgtc tgattttcaa attcaggaag
      601 atatttatga agcggttgat cgagttctat ctttccatct tctaatagac gtaaaactaa
      661 tgaagtggtt gcaacgactt tcgtcagtga ggctgcatca aacaacagat cttctgtcat
      721 cagctgcttt tcaggaacga cttgggcaaa gccggctgta aatgtttcta cttgattttc
      781 ttttatgaag gcgactgcta cacctggata aaatccttct tccatctttt tcaaaataag
      841 ctgtttcgtg tgtggataca tctttttctc ctgcttttct atcagattct tacagtccaa
      901 gcttaaagct taatctatga ggaatcaacc ttttttacac aaaaaaactg cgcgaaaaac
      961 cgcgcagttt cccccggtat actattttct ctttctatct ctagaataag cgatagtatg
     1021 accgttcata aaatgacgag acatgcgaca atcgttaaga aagaaaaagt taagcgtcgc
     1081 atcgaatgtc agttttattc ttttcttttg gctcactgac gaaccaccat tcgattccta
     1141 tagcatcata gattgtgagt actgtgtgct ttttgtctaa gaagaaatca actgatagtt
     1201 gctgcaagtg agcaatcagt ccttccatgc tttcagtatc gatcatgaat ttcagaaaat
     1261 caagtccaat cacttcatct gtaggcaaat caattgcgcc tccgctagct tctgtcaaac
     1321 caacatggaa atctccttca ttcaatacat ggatgccctc ttcttcatta tgtaccttca
     1381 aaccaagtac atgattaaga aattggcgtt cttttgttaa gttagtaaca tttaaatgga
     1441 ttttttcaaa ataagaacct gttgatagtt tactgaattt ttcttcagat tttcttaata
     1501 atgcttcttg atccattgtt tctggaattt ctctttggtc gttcttattc tcttcatggt
     1561 aaatttccaa ttcattctct tctgggtcta cgagcaaaat ccccatacgt ccatgatctt
     1621 ctaaagcttc tttgattgga taattcattt tacggatacg acaaaggata tctgccattt
     1681 cttctgtgct agggataatc aatgaaaaac ggttcagctt tttgatcacc cccatgtgat
     1741 cattcgctct aggactttct tccagcagca acatttcact tccagcttct tctgttccta
     1801 aaatggctaa ttcgttttct tctcttttca aatcaaagcc aacaacgttc ttgtaaaaat
     1861 caatcatttt atctcggtct ttcacccgaa tggctacact ttttaaatag gtggcagccc
     1921 ccaattgaaa atctttcata tttcttgctc ccctctacgt caagctttat tttaacgatg
     1981 gattcttctt ctttcaagca ataactttga ttttgttaaa aaaaatgtga gaaaattcgc
     2041 attttgcttg ttttttgtaa attaatgagt aaaaactctt ataaaaagag gttgtgaaaa
     2101 agctgttctg catcaagtaa taagaacagc caaacaaaaa tagctcctcc t
//
LOCUS       DASJPH010000196         2057 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJPH010000196 DASJPH010000000
VERSION     DASJPH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.204"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U3995_002916"
     CDS             200..742
                     /locus_tag="U3995_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798185.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="U3995_002917"
     CDS             898..1347
                     /locus_tag="U3995_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1798186.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2057)
                     /locus_tag="U3995_002918"
     CDS             complement(1377..>2057)
                     /locus_tag="U3995_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN1798187.1"
                     /translation="GLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLD
                     RTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFG
                     RIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVK
                     VEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERG
                     SDTSGEAA"
BASE COUNT          737 a          327 c          396 g          597 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
     2041 cgactgagtt gaagccc
//
LOCUS       DASJPH010000197         2049 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJPH010000197 DASJPH010000000
VERSION     DASJPH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.205"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..260
                     /locus_tag="U3995_002919"
     CDS             <1..260
                     /locus_tag="U3995_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1798188.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            261..1013
                     /locus_tag="U3995_002920"
     CDS             261..1013
                     /locus_tag="U3995_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1798189.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1018..1965
                     /locus_tag="U3995_002921"
     CDS             1018..1965
                     /locus_tag="U3995_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1798190.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          718 a          271 c          402 g          658 t
ORIGIN      
        1 tagaaattca tttagattat tttggaagag ttcctacaag aaaatttcaa ttgtttactt
       61 tagatgaatg tatcaatgtt gacttattaa ataataaaat aagttttgaa gaaaacattg
      121 ttaaagaaat gcctgaatct gctaatgata tgtatgagaa ggagttgaaa tatttctttg
      181 aaaacgtagt aacaggcaaa gaaaactgga atgatttata tcatgcaaac gaagtattga
      241 aaatagctga ggagaaataa atggaaattt tatttactat ttgtggtaga gctgggtcta
      301 aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa gcccctagtc catttcgctt
      361 tagcaatgat agatatctat caacaaaaac atcctgaaca taattgcacg acttgtttga
      421 atacggacag taatgagtta gtagaattga cgactaagtt aaatccgtct gtgaaaattg
      481 tagaacgtaa acctgaatta gctacagata ctgtagggaa aattgaagtg atacgtgatt
      541 cattcttaca actagaaaga gaagataata tatttgattt aattatagat ttagatttga
      601 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
      661 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
      721 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
      781 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
      841 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
      901 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
      961 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
     1021 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
     1081 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
     1141 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
     1201 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
     1261 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
     1321 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
     1381 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
     1441 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
     1501 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
     1561 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
     1621 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
     1681 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
     1741 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
     1801 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
     1861 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
     1921 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
     1981 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
     2041 tctgggata
//
LOCUS       DASJPH010000198         2044 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJPH010000198 DASJPH010000000
VERSION     DASJPH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.206"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..996)
                     /locus_tag="U3995_002922"
     CDS             complement(1..996)
                     /locus_tag="U3995_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1798191.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1014..1568)
                     /gene="srlA"
                     /locus_tag="U3995_002923"
     CDS             complement(1014..1568)
                     /gene="srlA"
                     /locus_tag="U3995_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1798192.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(1556..2038)
                     /locus_tag="U3995_002924"
     CDS             complement(1556..2038)
                     /locus_tag="U3995_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HEN1798193.1"
                     /translation="MYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAIGKR
                     PGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPSVRA
                     EDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKRWIS
                     "
BASE COUNT          584 a          459 c          345 g          656 t
ORIGIN      
        1 tcagctatat aatccaaaac ttgctaccca agcaatgata actcgtggga tgccgttaag
       61 aaatcttgaa taaagaactg atggtacacc tacttctact gtctcagctt ttgcttcttc
      121 caaacctaga ccgactggaa taaaatcaca tgcattttga gtattgatag caaatagtgc
      181 cggtaatgct aattggggag gaatatttcc tttcccaatc tctacaccga tcaatgttcc
      241 aatcacttgg ctaataactg cccctggacc aagcaaagga gataaaaatg gcagtgaaca
      301 aataaagcca atgattgtta atccaactgc atttcctgct aaaggtgcca taatgttagc
      361 aaatacatta ccgatcccag aaccttgtat cacaccaatc aaaagggaaa cgaaagccat
      421 aaaagggatc actgtccgga tcatcgtatc tactgcttct cttgctgatt gattgaatgt
      481 agcaactact tttcccgcgc ccattccaat ttttgctatc cagttttgct gctctgcttt
      541 ttgctcggtt atctttttag aagtatcata tttgtacttt ttatctggag atgcagtatt
      601 tactgtttct ttgttatcat cagtttccat tagtggctgt atttgttcta accctactgc
      661 tgaaacataa atatcttcag tgatatattg tgctaatggg ccactttttc ctgtggaaac
      721 gatattgatg gttggtatcc cttttttagg ataaattcca catctaagag tccctccaca
      781 gtcaacgatt gcaagcgcga tttcactatc aggtattgat gttttgaacc cattgataga
      841 ctccatacct gtcagttctt ctattttatc tacgatcatt ggcctctcac ctccacctgt
      901 gacatagatg aacttgtgtt tatcttttgt cggcctaata atcaaaggac ctccataccc
      961 gccgtttcct cttacgatct taatatcacc atacatttaa aactctcctt tctctaatca
     1021 actgaaagct ctactttttt gctaagaacg attccttgct gtctgcatac aaatccagta
     1081 gtaaaatcag ttatccaacc accaataaag ttcatcagca gtcccacaag caagtatcgg
     1141 atagccagct ccatcgaatt tagccctaag gtttcgatcc cttgagcaat ccccatccat
     1201 acaaacaatt ctccaggatt aatatggggg aatacaccat tgctcgtatg acaaaattga
     1261 gcttgagcag cataatagct tggtttgtag tattctggaa ggaaacgagc cattgtaaat
     1321 gacataggat ttcccaacat aaaagcagat aagaacggta acacaaggta acgagtaagc
     1381 ggatttttcg ctgataattt ggctactttc gttactcggt cttcacctaa aaaagcaatc
     1441 agcgtattca ttgcaaccat cagcatcaag acaactggta cgattcctgt catccagctt
     1501 atgaatgttt ctgcacctgt ttggaacagc ctcataaatc cctcagcaaa ttttgttatg
     1561 atatccatcg tttgtcactc cttttttctt tgatcgtact tttccatatc cgaaaattat
     1621 tgagcatagc aataaaaggc aatgaaggtt gttcactttt ttcatttcct gcctctattc
     1681 gtaagtatac ttctctggca ttttctaaag ctgcttgtgt caaacgatct tccgctcgaa
     1741 ctgagggatg gtaacgatcg atgtaatgga tatcttcacc cgcatagaca ggtaatgaat
     1801 gaaattttgc caaagatgta accccttgca tttttctgac ttctaaaact tgatcatgat
     1861 cgtctatagc tagcataaca atcgtgcctg aacggatttt cccaggtcgt ttaccaatcg
     1921 ctacttttcc ttttcttcgc agttcgctat atgtttgatt gaaatgcttt agttgcttcc
     1981 aactgataac tgcttgcagc acataagctg aagctgcgaa aactatgaat agatacatat
     2041 catt
//
LOCUS       DASJPH010000199         2034 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJPH010000199 DASJPH010000000
VERSION     DASJPH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.207"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="U3995_002925"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="U3995_002925"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1798194.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="U3995_002926"
     CDS             complement(1431..1823)
                     /locus_tag="U3995_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1798195.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DASJPH010000200         2019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJPH010000200 DASJPH010000000
VERSION     DASJPH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.208"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="U3995_002927"
     CDS             100..570
                     /locus_tag="U3995_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1798196.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="U3995_002928"
     CDS             792..1895
                     /locus_tag="U3995_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1798197.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DASJPH010000201         1993 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJPH010000201 DASJPH010000000
VERSION     DASJPH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.209"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="U3995_002929"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="U3995_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1798198.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="U3995_002930"
     CDS             852..1040
                     /locus_tag="U3995_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1798199.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="U3995_002931"
     CDS             1053..1610
                     /locus_tag="U3995_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1798200.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="U3995_002932"
     CDS             1642..1884
                     /locus_tag="U3995_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1798201.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DASJPH010000202         1979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJPH010000202 DASJPH010000000
VERSION     DASJPH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.210"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="U3995_002933"
     CDS             complement(325..528)
                     /locus_tag="U3995_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798202.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="U3995_002934"
     CDS             complement(728..1744)
                     /locus_tag="U3995_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1798203.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DASJPH010000203         1845 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJPH010000203 DASJPH010000000
VERSION     DASJPH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.211"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            192..389
                     /locus_tag="U3995_002935"
     CDS             192..389
                     /locus_tag="U3995_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798204.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            495..977
                     /locus_tag="U3995_002936"
     CDS             495..977
                     /locus_tag="U3995_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798205.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1035..1631)
                     /locus_tag="U3995_002937"
     CDS             complement(1035..1631)
                     /locus_tag="U3995_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1798206.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          575 a          276 c          368 g          626 t
ORIGIN      
        1 ggaatatgtt ttaatttttt gaaaaaaatt cttctaaata caacagctgt tttaattgtg
       61 aaaacgaaaa cagtacgcta taatgcctgt aaggttgatt tttaactgta aataaaaatc
      121 aatgagttaa atctccaaag aaatagcagt ggtaatgtgg agaaaagttc cagcagttta
      181 ggaggatttt gatgacggaa gaaatgatta atttaggaga acaatatgca tgcaaaccaa
      241 ttggttttac aaaaacggtc attggtgaag ttgtatcaaa aatgacaaac tgcgccgtgg
      301 tcaaagttgc ccaatgtgct gcagaggacc aagaattatt agatgaaaaa gcaagcatgg
      361 ttgttgcaaa atatgataca tttgaataaa gaaaacaatt ggacgtgcgg cgtgctgcaa
      421 gtccaatttc tttgagaagg taaatcagta ttcttttcct attttctgga gaagaaaaag
      481 cgaggcgagt tgtcatggta acattcttta cggttctatt gtttttattc gttgttgtga
      541 tgtttttatt tctttggctg gttcgaaaag aaattattta tcggactgtg cgaaatcgat
      601 gggtctactt ggtgataccc ttcttagtgt cactagtcat ttggtataca ctcatcagtc
      661 atcctacagc agatgaattg gcaaaaggga tcttgtcagc aatgattttt attagttttt
      721 tgttggacag tagaggaatc acagaagaag gacttgtttt gaatagcttt gacaaaaaag
      781 gtgttccttt ttctgagatc aataaaattg tattatatca gccaaaaggt tctaaaatcg
      841 taaaaatgaa ttttttccga aacggatggc gaggaccgat gctgaaattt tctgcatcat
      901 tagaagaatt agttccattt ttgtcccaac gcttaaacga tgaggcagag attgatatta
      961 tgattgatcc agaataaaaa cagaggttgc gacaagatgt ttgtcacaac ctctgttttt
     1021 tatttcaaat aaaattatcc aaatacgcgg aagcgatcgg catcgttcat gtattctttt
     1081 tcctcagccg gtgtttctgg tgaaccgaat actaattgcg aacgtagttt ccagtttgaa
     1141 gggatattcc attcttttgc aactgcttcg tcaataactg ggttgtaatg ttgcaagttt
     1201 gcgcccaatc ctttatcaac taatgctacc caagtattag ctgttgcgat tccgtttgat
     1261 tgttctgacc aatctgggaa gttgtctgca tataattcaa attgttcttg tagacctttt
     1321 accacgtcag tatctttgaa gaataatact gtaccataac catttttgaa accagctaat
     1381 ttgttttgtg tatttgggaa agcttctgct ggagtcaatg ggcgaagtgc ttcttcagtg
     1441 atttcccaaa gtttttcatg tgcatctccg aaaaggataa ctgcgcgtgt tgattgagca
     1501 ttgaaagcag ttgggctttc tttaattgct tctttgatca aagttgttaa ttcttcatta
     1561 gaaagtgaaa cattacgtcc tagatgatag attgaacggc ggtttttcaa agtgtcagta
     1621 aaagttgtca taataaaatt tctcctttga tttgcgtttt gcattttttt aattgattac
     1681 gaaatgaagt atagcacctt acaaaaagta agtaaacaga tatgctcaca aaaaataagc
     1741 gaagttcaaa aaacaatttt aataagaaaa atcgttatgt aaggattttc ttagttatcg
     1801 ctttcattgt tgtgttttag gaatttttgc ttatttgaaa aaaac
//
LOCUS       DASJPH010000204         1771 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJPH010000204 DASJPH010000000
VERSION     DASJPH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.212"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..555
                     /locus_tag="U3995_002938"
     CDS             <1..555
                     /locus_tag="U3995_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798207.1"
                     /translation="NIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDG
                     YGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSS
                     TLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNR
                     LSEEKILLPENWAELRKLVEYGII"
     gene            542..>1771
                     /locus_tag="U3995_002939"
     CDS             542..>1771
                     /locus_tag="U3995_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1798208.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKI"
BASE COUNT          586 a          229 c          278 g          678 t
ORIGIN      
        1 aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa catacaatca
       61 atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga tcaaccttta
      121 ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa tagtatattt
      181 tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa tcgttatggt
      241 gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga aaatgacttc
      301 tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc tatgatgttt
      361 gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata tacagcatta
      421 tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca ttataaccgt
      481 ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa gttggtagag
      541 tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt tctggtatac
      601 tatagggaat ttttttatta agggaatttc attcataact attcctattt ttgtaaatat
      661 tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat caattttttc
      721 gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg actataaaga
      781 tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt taattgtgtt
      841 cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata tgaaccaact
      901 tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt atatccagtc
      961 ttattttacg attaacttta accattttaa atatttgatt ttatctttta tatctacttt
     1021 tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg ataaatatgt
     1081 tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa tttatttaag
     1141 aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg ctttaaaaat
     1201 ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat tcgatagaat
     1261 tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat
     1321 aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta
     1381 tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt
     1441 agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat
     1501 ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt
     1561 ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt
     1621 tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat
     1681 acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt ttttactttt
     1741 tatttttcat tataatatcg ctaaaaaaat t
//
LOCUS       DASJPH010000205         1756 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJPH010000205 DASJPH010000000
VERSION     DASJPH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.213"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(192..1484)
                     /locus_tag="U3995_002940"
     CDS             complement(192..1484)
                     /locus_tag="U3995_002940"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1798209.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          505 a          370 c          308 g          573 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctacttttgg
     1741 aacaccgtga atcaga
//
LOCUS       DASJPH010000206         1725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJPH010000206 DASJPH010000000
VERSION     DASJPH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.214"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1633
                     /locus_tag="U3995_002941"
     CDS             155..1633
                     /locus_tag="U3995_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1798210.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          634 a          236 c          307 g          548 t
ORIGIN      
        1 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
       61 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      121 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      181 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      241 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      301 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      361 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      421 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      481 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      541 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      601 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      661 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      721 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      781 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      841 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
      901 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
      961 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1021 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1081 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1141 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1201 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1261 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1321 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1381 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1441 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1501 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1561 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1621 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1681 gtttttttca aagaaaaggt aactagatat aactagatat aacta
//
LOCUS       DASJPH010000207         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJPH010000207 DASJPH010000000
VERSION     DASJPH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.215"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U3995_002942"
     CDS             131..505
                     /locus_tag="U3995_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1798211.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U3995_002943"
     CDS             complement(619..1248)
                     /locus_tag="U3995_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1798212.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U3995_002944"
     tRNA            complement(1382..1454)
                     /locus_tag="U3995_002944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJPH010000208         1661 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJPH010000208 DASJPH010000000
VERSION     DASJPH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.216"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..252)
                     /locus_tag="U3995_002945"
     CDS             complement(<1..252)
                     /locus_tag="U3995_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798213.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(254..1312)
                     /locus_tag="U3995_002946"
     CDS             complement(254..1312)
                     /locus_tag="U3995_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1798214.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          657 a          275 c          195 g          534 t
ORIGIN      
        1 atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa aggaagaacc
       61 gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt ttatacgctc
      121 actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt ttgattctcg
      181 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
      241 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      301 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      361 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      421 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      481 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      541 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      601 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      661 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      721 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      781 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      841 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      901 tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa
      961 gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa
     1021 tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt
     1081 ttgaaattaa tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa
     1141 catttggact tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta
     1201 atcctggcgt tcctgttggg acatgataag aaatcaaaaa aataaattga tagatagata
     1261 ttagcgcagc taaaaaaatt atgataatga aaacattatt aaaatacacc atttttttct
     1321 ttttttcctc ttcactttgt gaaaagtctg caggaaaaat tactatcaaa gtaatcagca
     1381 aatatccaaa attataaata gatggaatta aaaaattttc tctgttaatg attatagtta
     1441 agataaaact aatcattgct ccaatcagta gccaagaaaa acgcatttta aaaaaatttt
     1501 tctttataaa aacctcatac aaaactataa ttccacccca agcaaaagct attttcatcg
     1561 ggatattagt tacattaaaa gtactaggca taaaataaag cagacaaatg attaaattta
     1621 ttactcgaaa cgcatctcga cttaataaac tcttctctaa a
//
LOCUS       DASJPH010000209         1649 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJPH010000209 DASJPH010000000
VERSION     DASJPH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.217"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..424)
                     /locus_tag="U3995_002947"
     CDS             complement(<1..424)
                     /locus_tag="U3995_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1798215.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="U3995_002948"
     CDS             complement(533..1564)
                     /locus_tag="U3995_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1798216.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          510 a          287 c          286 g          566 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttagga
//
LOCUS       DASJPH010000210         1636 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJPH010000210 DASJPH010000000
VERSION     DASJPH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.218"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1502)
                     /locus_tag="U3995_002949"
     CDS             complement(183..1502)
                     /locus_tag="U3995_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN1798217.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DASJPH010000211         1631 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJPH010000211 DASJPH010000000
VERSION     DASJPH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.219"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..860
                     /locus_tag="U3995_002950"
     CDS             <1..860
                     /locus_tag="U3995_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEN1798218.1"
                     /translation="GAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLY
                     GQMADYERILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSG
                     GGALVSSDKKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDR
                     VAKRREIFNRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLN
                     EKNIETRALWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFV
                     IDEFKKIFK"
     gene            873..>1631
                     /locus_tag="U3995_002951"
     CDS             873..>1631
                     /locus_tag="U3995_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1798219.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDS"
BASE COUNT          594 a          210 c          317 g          510 t
ORIGIN      
        1 aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg agccctgttg
       61 cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg
      121 tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat
      181 ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt
      241 cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa
      301 cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt
      361 tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt
      421 accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata
      481 gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct ggaattgata
      541 gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa
      601 cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa
      661 aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg
      721 taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct
      781 tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt
      841 ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt
      901 ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg
      961 gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt
     1021 ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat
     1081 cccctataac ccatgaattt attataaaag aggctttaaa tttagattgt aatatcttta
     1141 cggaaatcaa tttattaaat gaatattatg atgaaataat cgaagtagct aaaaagaaag
     1201 atagattact ttatctatct tctacatttt tgaagagaaa agaaattcaa tatattaagt
     1261 ctaaagtgac gcataataaa aaaatttctt atcaatacca tgtaggacag tatttacccg
     1321 actggcatcc ttgggaagat tatcgcaatt tttttgtttc aaacaaaaaa actaatgggt
     1381 gtagagaaat attcgctatt gaattacctt ggattataga tacatttggg aaagttaaat
     1441 cgtttactgt aatgacccag aaattatcca atcttgatat tgattattcg gacagtttca
     1501 ctcttattat tgagcatgaa actggtatag ttggaacttt aagtgtagat gttgtttcaa
     1561 gagtagctaa aagaaattta actataattg gtgaaaagat tttaatagaa tgggaaggta
     1621 acccggattc t
//
LOCUS       DASJPH010000212         1594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJPH010000212 DASJPH010000000
VERSION     DASJPH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.220"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="U3995_002952"
     CDS             288..695
                     /locus_tag="U3995_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798220.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="U3995_002953"
     CDS             709..1110
                     /locus_tag="U3995_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798221.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="U3995_002954"
     CDS             1112..1483
                     /locus_tag="U3995_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798222.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DASJPH010000213         1564 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJPH010000213 DASJPH010000000
VERSION     DASJPH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.221"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..135
                     /locus_tag="U3995_002955"
     CDS             <1..135
                     /locus_tag="U3995_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1798223.1"
                     /translation="VIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK
                     "
     gene            148..1056
                     /locus_tag="U3995_002956"
     CDS             148..1056
                     /locus_tag="U3995_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN1798224.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1056..>1564
                     /locus_tag="U3995_002957"
     CDS             1056..>1564
                     /locus_tag="U3995_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798225.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          554 a          208 c          339 g          463 t
ORIGIN      
        1 gttatccgag gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt
       61 atgatttgtg gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc
      121 ataccaccaa aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca
      181 gaaccctata attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt
      241 atggaagcta caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat
      301 tttgaatcag ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct
      361 aaaaaaggga aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa
      421 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc
      481 tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
      541 attgaagatt taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg
      601 gactggatga atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt
      661 ttccaagatt ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag
      721 gatattttga agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt
      781 gtagatcttc aaggtgtacg ccatgaatcg atttctgaac gtttcctatc cgatctaaat
      841 tatttgtatc aaaaaatgaa agcagcactt ttcacgattg aatacggtga aattgaagtg
      901 actgatattc ttcaagacga tctcttttca gataatcacg aagtcgaaaa aattgaaaca
      961 gtagaatttc caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag
     1021 aatgttttca ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg
     1081 agactgatga atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt
     1141 taggcttaga aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg
     1201 gtgcagtcat tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt
     1261 atgcaagcga gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg
     1321 gagaaggtgg aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag
     1381 ttggggaact cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact
     1441 tcaatttctc tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg
     1501 caaatatatc aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg
     1561 gtgt
//
LOCUS       DASJPH010000214         1558 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJPH010000214 DASJPH010000000
VERSION     DASJPH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.222"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1558
                     /locus_tag="U3995_002958"
     CDS             <1..>1558
                     /locus_tag="U3995_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1798226.1"
                     /translation="DFNSASKRIAYLLNRLIETEDFLRMDDLSEEVGVSRGTVVKDLK
                     TLKEMINDFDVKITGTPNKGLRIIGDELELRLLLFYFVSPYFSETKTEQFLEEKQAIK
                     HFQKKTNASKQEITLLTKVIGISLNRISSNYLLKKPINRYSGCFEYIQEFNELINQLE
                     EIYELTLSQYEKDFISFPLNIINTSITMIRKANSDLRLYFDPMMMRIRRLDLIDLDES
                     FLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKKYPFSYQIASECIQALEAILQHKIS
                     KVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTGKGTALMIQRQIKRILGRDIQITHV
                     SEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHLTNLFDDSWLRAQWQKAKKKRLAGF
                     HSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVDEEFEKRILKREYQQSTFVNKEFAF
                     PHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFLFAIPEEMTAEVENELFELYDILFE
                     LIKMGIDEFIE"
BASE COUNT          572 a          239 c          285 g          462 t
ORIGIN      
        1 tgatttcaat tcggcatcca aaagaatcgc ttatttacta aataggctga tcgaaacaga
       61 ggatttctta cgtatggatg atttgtctga agaagtcggt gtcagcagag gaacagtggt
      121 caaggatcta aagacattaa aagagatgat caatgatttt gatgtaaaga ttacaggcac
      181 accaaataaa ggactaagaa taataggaga tgagctagaa ttacgactac ttttatttta
      241 ttttgtttct ccctactttt cagagacgaa aacagaacag tttctagaag aaaaacaagc
      301 aatcaaacat tttcagaaga agacgaatgc tagtaaacaa gaaatcaccc ttttaacgaa
      361 agttatcggt atttctttga atagaatctc tagtaactat ctgttgaaga agcccatcaa
      421 tcgttacagt gggtgttttg aatatatcca agaattcaat gaactgatca atcagttgga
      481 agaaatctat gaactaactt tgagccaata tgaaaaagat tttatcagtt ttcctttgaa
      541 catcatcaac acaagtataa cgatgataag gaaagctaac tctgatttgc gcttatattt
      601 cgaccccatg atgatgcgta ttcgacgatt agatctcatt gatctggatg agtccttctt
      661 atatcaagag atgaaacagc acctctcttt attgataaat cgggtaattt ttcgttatcg
      721 attgactgat ttattttatg gtgagataga aaaaaagtat cctttttctt accaaatcgc
      781 tagcgaatgc atacaagcat tagaagctat cttgcaacat aagatctcta aagtagagat
      841 cagctaccta acgttgtatt tggagttagc atttcgatat caaaaagagc atttttcctc
      901 taaaaaagta gccattgttc atacgacagg aaaaggaaca gcattaatga tccaaagaca
      961 aatcaaaagg atattgggga gggatataca aataacacat gtttcagaag aacaatatga
     1021 aaaagtagat ctaaacaaat atttcgctgt ttttacaacg attccgttaa aaaatgtaaa
     1081 tgaagagaca ccggtgatcc atttgactaa tctttttgat gactcttggt taagggcaca
     1141 atggcaaaaa gcaaaaaaaa agcgattagc cgggtttcat tctactgttt ttttctatca
     1201 aatatggaaa gaaaaaaaga cgtatcaaga atatttaaac aaattagtcg ctcctttgat
     1261 cagaggctat tatgtggatg aagaatttga aaaaaggatc ttgaaaagag aatatcagca
     1321 aagcacattt gttaacaaag aatttgcttt tcctcacaca accaaccagc tatctcaaca
     1381 cattgtttta tcagtagggc taataactga tcaaggttta gcagaaacag gaggaaatgg
     1441 agttattttt ttatttgcca tacctgaaga aatgacggct gaagttgaga atgaattatt
     1501 tgagctatac gatatcctat ttgaactcat aaaaatggga atagatgagt ttattgaa
//
LOCUS       DASJPH010000215         1540 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJPH010000215 DASJPH010000000
VERSION     DASJPH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.223"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            25..1491
                     /locus_tag="U3995_002959"
     CDS             25..1491
                     /locus_tag="U3995_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1798227.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          423 a          289 c          326 g          502 t
ORIGIN      
        1 atttaaagaa ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag
       61 ccgaagggat tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt
      121 atgcgtgcat tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta
      181 ccacgagcaa cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt
      241 atcgtcggcg gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt
      301 ggagttttca ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg
      361 tttgtttcga tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc
      421 atggtcggac atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt
      481 tacatgggtg tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa
      541 gaatataact atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta
      601 caatacatca tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct
      661 ttgacaactg aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc
      721 gctgcagtat tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat
      781 acaatcagtg tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca
      841 aaagatgtaa ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca
      901 gcaatcattt tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt
      961 gaacgtacac aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg
     1021 aatccaattt tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt
     1081 gaacgtcaac catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg
     1141 tacttgctat tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg
     1201 tggttgttgg gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt
     1261 ttgtctgtga caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg
     1321 ttcttagctg atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg
     1381 ggaacagaat ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg
     1441 ctatttactg tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac
     1501 ataaaaacag gaattgctta tttgcagcag ttcctgtttt
//
LOCUS       DASJPH010000216         1536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJPH010000216 DASJPH010000000
VERSION     DASJPH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.224"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="U3995_002960"
     rRNA            complement(<1..>1536)
                     /locus_tag="U3995_002960"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DASJPH010000217         1476 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJPH010000217 DASJPH010000000
VERSION     DASJPH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.225"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..277
                     /locus_tag="U3995_002961"
     CDS             23..277
                     /locus_tag="U3995_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798228.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            347..592
                     /locus_tag="U3995_002962"
     CDS             347..592
                     /locus_tag="U3995_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798229.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            668..1033
                     /locus_tag="U3995_002963"
     CDS             668..1033
                     /locus_tag="U3995_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798230.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT          415 a          270 c          273 g          518 t
ORIGIN      
        1 gaatcgttga ggagtccgtt acatgattac gttggtgaga gttttgtttt gggtgcctgc
       61 ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg aacaaagaac ggttttattt
      121 agctattttg actcttccgg ctatctactt tatgtggaaa gtttttaatt acaactactt
      181 tgaacccgat agtgttttta tagaagaatt atcaggactg gttttgtctt tattgatcgt
      241 cattctttat ttgatacgtt taaataaaaa acattgagta ctattgattg catgtgtagc
      301 tttctatagt cattataact attctactat aatgaaaggt gattaaatga gtatcgaaga
      361 agctatttta ctggatactt tacgttgtcg tctgaatttc ttaagaactg gccgatcttt
      421 atctcaagta gctgtttcta gcgcgttaag tattccttta ttcaaatatc gtaagtacga
      481 agaaggagaa gaaattccaa gcatcattga attaatctat atcgctgact tttacgatat
      541 ttccgttgat tacctaattg gtcgctcaga aacagcaagc aaactgaagt gattttttcc
      601 agccttggtg ctggtttttt catttaacgt ctaaggttaa caatctatat ataaaggaga
      661 catttctatg cgattatggc atgaaaaatt gatttcaaag cttcctcgtc aacagctttt
      721 aggacaacat cgggaatgct gtgcattaag aggaaatggc tggggaaaga aacatgctac
      781 tgtggattat gtttttcact attctcctta tagattattc caatatcatc agcttgtcat
      841 ggatgaaatg gaaaaacgtg gttacagccc agcaccagag tggaaaaatc ctatgtatcg
      901 tggcaaatca tgcgaagcat tttttgattt acctactgta gagcgcaacg tgccgattta
      961 tccggaacac gatgaagttt atctagcaga atgtttagca aatttggagc aaaaaggaat
     1021 ttatcttacc taatataact agaaaagcct tctatctcct cttacttcat taaaaagctt
     1081 taaccatctt agatcaagct aaaatgtcgt aagttttagg gttatcttca tcctcacgtg
     1141 caaaactaaa agcgtactct agttttggtt catcgccaac ttccctagac ataaatgaac
     1201 atctcctgac gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat gcacagcaca
     1261 gattgcttct tttgatcttt cttttgattt ttctgttgct tcttctacac cttcttcgat
     1321 tactacatcg atgcttacaa ttgcttctgc ctaatattcc catgcatcgt cgatttaagc
     1381 ctctgtagtc actgtgatcg tttccacatt ttaactcctc tgtcgtggct tcagttgatt
     1441 ttgtgatcag ttcagttgct aagccagagt cagaac
//
LOCUS       DASJPH010000218         1446 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJPH010000218 DASJPH010000000
VERSION     DASJPH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.226"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(942..1355)
                     /locus_tag="U3995_002964"
     CDS             complement(942..1355)
                     /locus_tag="U3995_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1798231.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          531 a          238 c          214 g          463 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctt
//
LOCUS       DASJPH010000219         1367 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJPH010000219 DASJPH010000000
VERSION     DASJPH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.227"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..1144
                     /locus_tag="U3995_002965"
     CDS             137..1144
                     /locus_tag="U3995_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1798232.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1156..>1367
                     /locus_tag="U3995_002966"
     CDS             1156..>1367
                     /locus_tag="U3995_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1798233.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFE"
BASE COUNT          534 a          171 c          253 g          409 t
ORIGIN      
        1 gatacttcat attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat
       61 aattagttta gttatatatt ttttaaagat taagaatcga attatagatg actataattc
      121 ataggaggag atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc
      181 cattggaagt gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta
      241 tagtagaggt gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt
      301 gagattttta attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt
      361 ggatattatt tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat
      421 ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa
      481 taatgtaaaa cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat
      541 gggtgcgaca aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa
      601 aacagaaaca atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt
      661 tattcctctt tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga
      721 tatgacacga tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat
      781 gaattcaaaa tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt
      841 agctcaatca ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat
      901 aaaagttgta ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga
      961 aattgaacgc tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac
     1021 tgacgaactg tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac
     1081 agaattaatg agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg
     1141 gtaaggagtt ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat
     1201 aatggaagtc tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac
     1261 gcggttaaat ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca
     1321 aaatatcaga aggataatct taaagaggac agttctcaat ttgaaat
//
LOCUS       DASJPH010000220         1340 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJPH010000220 DASJPH010000000
VERSION     DASJPH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.228"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1306
                     /locus_tag="U3995_002967"
     CDS             5..1306
                     /locus_tag="U3995_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1798234.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          433 a          267 c          248 g          392 t
ORIGIN      
        1 ctgtatgcct gatcttcatc atatcaaact actacttgga atcaaagata aaaacatttt
       61 catcacaaat gtggaatcga agagcattaa gaatatcaag agtcttgttg tttctgcgac
      121 gttaagtaaa gaaattcgtc gctgtccttt gtgtaaacag atgaatcatg aaggcatgat
      181 cgttaagaac ggaaagaaaa aatcgctcat tcaacttaat aaatgtgcga accaactgac
      241 ttatcttgct ttagcgaaac aacgctacca ctgtcgtggc tgccacacct attttacggc
      301 gaatacctac attgtcgata gaaactgctt tatcgctaaa caagtgcgct ataaaatttt
      361 agaagagcta actgaaaaac aagcaatgac gacaattgct aagcattgcg gcgtttcttg
      421 gtcgaccgtt tctcggacac tcgcttctct gatccccatg acaaaagtaa aaagaaattg
      481 gttaccgcgt tgtttattaa tggatgaatt tcgttcactg aaaaatcaag tcggtcccta
      541 ctccttttct tgtatggatg gcgatactgg aaaattactg gatattcttc cttcacggaa
      601 gaaaaaggct ctcgtttctt attttatgca atttgaacga cgcgcacgat tgaaggtaaa
      661 ggtcatcgtg acggacatga atgtttctta tgcctctctg ataaaggaat gttttccaaa
      721 ggcaaaactc gttgtggatc gttttcacat tgtcaaacat ctcattcgta aatttgagga
      781 tattcgcgta cgaatcatga aaagctttga tcgaaacgat ccaacacaag ccaaatatta
      841 tcgacaatta aaggcgttgt ctcggcttct gatcaaacga caggacaagc ttgtttatga
      901 caaatggacc aaatggcgca attttggttg ggcgtatctc accgaaagtg aagtggtgga
      961 tcgtctgctt tcgatctctg acgaattacg aatcgcctat ttttactatc aagaaattct
     1021 tcaagcgttt cacgacaaag aggcggacac tttctttaaa cttgtaagaa cgatgcccaa
     1081 ttccgttcca aaagaactgc attctataaa aaaagctttt ataaactatg aatccggcat
     1141 tcgtttagcc ctcgaattgc cttattcaaa tgccaaaata gaaaatctgc atacccacat
     1201 caaagcattg aaacgagtcg cttatggctt tcgaagcttt cggaaaatga agaccagaat
     1261 tttcttacta aataatctga ttacctatga atcaaaaaac atctaaagta gaaaaggaat
     1321 tctaccttag atgttcttat
//
LOCUS       DASJPH010000221         1339 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJPH010000221 DASJPH010000000
VERSION     DASJPH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.229"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U3995_002968"
     CDS             <1..828
                     /locus_tag="U3995_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN1798235.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..>1339
                     /locus_tag="U3995_002969"
     CDS             828..>1339
                     /locus_tag="U3995_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798236.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          460 a          193 c          314 g          372 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgt
//
LOCUS       DASJPH010000222         1334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJPH010000222 DASJPH010000000
VERSION     DASJPH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.230"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..1165
                     /locus_tag="U3995_002970"
     CDS             86..1165
                     /locus_tag="U3995_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798237.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DASJPH010000223         1299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJPH010000223 DASJPH010000000
VERSION     DASJPH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.231"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            143..400
                     /locus_tag="U3995_002971"
     CDS             143..400
                     /locus_tag="U3995_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798238.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="U3995_002972"
     CDS             complement(632..1063)
                     /locus_tag="U3995_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEN1798239.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          492 a          169 c          161 g          477 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacacataaa aataaaaaac
     1141 atttgtattt tatcaaagtt ttattatact tgtcaatttg tgtacatcct aaaatataag
     1201 ctatttgttt acttctgtag aatatatact ttgttgttag ataagttgca catgtttata
     1261 gaatctaaat aataaatttt tatgtaccgc ccctccaaa
//
LOCUS       DASJPH010000224         1294 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJPH010000224 DASJPH010000000
VERSION     DASJPH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.232"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="U3995_002973"
     CDS             <1..1244
                     /locus_tag="U3995_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1798240.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASJPH010000225         1280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJPH010000225 DASJPH010000000
VERSION     DASJPH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.233"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            919..>1280
                     /locus_tag="U3995_002974"
     CDS             919..>1280
                     /locus_tag="U3995_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1798241.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSV"
BASE COUNT          467 a          200 c          177 g          436 t
ORIGIN      
        1 gaaacaattt tttctgcttg ttacctaaat taaatcacaa ctaaaggtaa cagtcaacaa
       61 agatttgtta ctaatctaat tttctatttt ttcgccttcc aaatcaagaa aaccgaaggt
      121 aacgtcttat cctagtgtga tacctcaaat gtccactttt gttacctttg ctgtccaaaa
      181 ctgttaccct tactgtccac ttttgttacc tttactgtcc aaaagtgtta cctttatacc
      241 cctttaatac ttactctccc aagagattaa gcctccccta acagatatat taacagatat
      301 ttaacagata tattaacaga tatttaataa aggaggatta ctcctctctt atttcaaaat
      361 cttaaaaata aaaaatctta aaaaaaacta acactatcta ggagtttatt ttgcttgtgt
      421 aactttgaat aattgaaaaa aaggaaaagg aggaagcttc agcttcccct tttatttata
      481 tttataatat atattttaat ctttaaaact ttagcaacct ccaaaaaggc tttatatcaa
      541 tcttaaatag attttagggt ggacataaag cgatgcgaac atggacaaaa agcgatgtga
      601 atgtggacaa aaagcgatgc aaacgtggac aaaaagcgat gtggtattcc gaatatatcc
      661 aaaaaaagaa aaagtatgtg aatttatttc taaaaactag aaaaatattt gttttttaat
      721 tttggtaatt tttataattt cgtattttat tgaaaaggat ttttattttg aaaatgttct
      781 aaaatagcga tttctgctgt attttcaatg aaatttgatt ggaaaaaata aaatgtgttt
      841 tactgacatt gagttatgtt ataaatttac gatacatttc ttattgtaac gtatcgaaaa
      901 caggtctata cttattcaat ggaatcgtac cagttccaag tttttaagct cgtaccaagc
      961 ttagaaagaa gaggcaatcg tatgaaaata acagaaaaga atgtaaaccc gaaatttgaa
     1021 actaccgaaa gcaatctaaa aaagtttgat ttaactgtta ctttatcgaa tcaacttgta
     1081 gatagcattg ttgaaatgga tgctatgcca caaaaaattt atctttacgc tataggacat
     1141 cttgacccta aaaatccgcc ttttaataat accgtcaaag tatctaaaaa ggctttattt
     1201 gcgttgatgt cttctagttc atcttcttca aaacattcaa gattaaaaca agttattgaa
     1261 aaattgcatc agcaatctgt
//
LOCUS       DASJPH010000226         1278 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJPH010000226 DASJPH010000000
VERSION     DASJPH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.234"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U3995_002975"
     CDS             complement(31..1203)
                     /locus_tag="U3995_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN1798242.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          266 c          245 g          422 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatct
//
LOCUS       DASJPH010000227         1252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJPH010000227 DASJPH010000000
VERSION     DASJPH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.235"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..1111)
                     /locus_tag="U3995_002976"
     CDS             complement(407..1111)
                     /locus_tag="U3995_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798243.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          368 a          216 c          228 g          440 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccg cttttttctc tatcagaaaa tatgtttatt attagaattt
      181 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
      241 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
      301 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
      361 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
      421 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
      481 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
      541 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
      601 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
      661 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
      721 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
      781 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
      841 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
      901 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
      961 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     1021 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     1081 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     1141 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     1201 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DASJPH010000228         1213 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJPH010000228 DASJPH010000000
VERSION     DASJPH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.236"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            320..490
                     /locus_tag="U3995_002977"
     CDS             320..490
                     /locus_tag="U3995_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798244.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(914..>1213)
                     /locus_tag="U3995_002978"
     CDS             complement(914..>1213)
                     /locus_tag="U3995_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1798245.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          357 a          233 c          194 g          429 t
ORIGIN      
        1 agccattgga acgctcaaag ttattttatc cgtccaaggc actcgatttc aaatcatgtc
       61 agacataaaa tcacttcttt actgactgaa aaagtgtcgt tattttttat ttcgaaaagt
      121 tgttaggtat ctttgacaac cgttattcgc acattgaaag agttgagaag ctatttacca
      181 aagtcttcca aaagaattct gtcttgagtg ttgagggttg tgaatttcgt tctcacgttt
      241 ccgttgagga taaactgagt tttatttccg cggatggcga gacagaaaaa ttaatcgaga
      301 aaatttacct acacaaaaaa ttcctcgatt aacaaactat ttccttagct gcactaatcc
      361 taaagaaata gaattcttag ttacagacat gaacgccacc tactttcagc tcaccaaaca
      421 tgttctacca aatgcgaaat tggtgattga tcgattactt ggtttttcca cgcctttcaa
      481 ggaggcgtaa cctttattca cgagttggtt gaagctttcc gagacaaaag acccgacttg
      541 tttttctcct tattgacaaa gagccattaa aactagagaa aaaattacaa ctgatcgctg
      601 attatttgta gaaagaaaat caaagtatta cccaataaac ttgagtcatt aggcaaaaaa
      661 cactgcgaaa ctcaagaatg attttcgcag tgtttttgta tattttttat taactagtat
      721 tactacaaag gtttattttt cacttctcaa cgcagcacaa aattgtcttt acttatttac
      781 tggttgggtt caaccttctc tactccgata ctaatatttg tttgtatacc ccttctaaaa
      841 tcggtagttt ccattcaatt gatgggtact agttgatttt aaaaactatg cttattcaat
      901 tttttatttt agctcatata tttcgtttct ttctaaattt tatactgatg aaaacaagta
      961 gcacacccaa tattgacaat ataaactggc taatgatggt ttctccagtt tttggaagat
     1021 gcttatttcc actgcttgtt ggattgtttc ctcttatatc tgagctatca gcaacttttc
     1081 tattctcttt tgttaaaaag atcgccactt cttgttcttt ttcgatcttg aaagttactg
     1141 gtgtcgcatc caattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg
     1201 gtgctaagcc atc
//
LOCUS       DASJPH010000229         1208 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJPH010000229 DASJPH010000000
VERSION     DASJPH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.237"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1208
                     /locus_tag="U3995_002979"
     CDS             <1..>1208
                     /locus_tag="U3995_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1798246.1"
                     /translation="KMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRSCGS
                     TVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQP
                     RHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRV
                     LMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVT
                     DMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLK
                     KNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKN
                     THIKTMKRVS"
BASE COUNT          401 a          241 c          236 g          330 t
ORIGIN      
        1 aaaaatgctg agaataacag agaaagattt gatgattaca gaggtctctt acgagaccct
       61 tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag
      121 aagttgtggt tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatggaaa
      181 aaaggaaacg attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa
      241 aaagcaacgc tatacctgta aaaactgtcg cacccattgg acggctcaaa gttattttgt
      301 ccaacccaga cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga
      361 aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg
      421 tacattgaaa gagtttaaaa gctatttacc aaagcaatct aagaagatcc tccccagagt
      481 attgatggtt gacgaatttc gttcgcatgc ttccatagaa gataagatga gctttatttg
      541 cgcggatggc gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt
      601 aacaagctat ttcttaggtt gtaccaatcc agaagaagta gaattcttgg tgacagacat
      661 gaacgccgcc tacttccagc tcaccaaacg tgttctgcca aatgcgaaag tcgtgattga
      721 tcggtttcat attgtcaaac acatgaatca agcgttcaat gagttgcgta tccgtgaaat
      781 gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa agaactggcg
      841 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      901 ccgagcacca aaatacccat ttcttactga agcaatgatg attgatcgat tgcttgagtt
      961 ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag cctttcgaga
     1021 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacag
     1081 ctttcgggaa aagcttcaaa accttctgac ctatgaagaa ggcatcacca acgcaatgat
     1141 ctatccttat tccaatggaa aaatagaagc gaagaatacc cacataaaga caatgaaacg
     1201 agtatcct
//
LOCUS       DASJPH010000230         1207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJPH010000230 DASJPH010000000
VERSION     DASJPH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.238"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="U3995_002980"
                     /pseudo
     CDS             172..459
                     /locus_tag="U3995_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="U3995_002981"
     CDS             473..904
                     /locus_tag="U3995_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798247.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="U3995_002982"
     CDS             906..1043
                     /locus_tag="U3995_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1798248.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
BASE COUNT          445 a          173 c          252 g          337 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccattatgca ttacagacga agaagcagat aaaatcatta
     1021 ataaagataa gagcgcatct taattgatgc gtttttattt aaggtaaagg agttgtcaca
     1081 tgattaattt aggggaatgg ggaatgatag caggatcaat aaccgctatc gtttctttga
     1141 ttttattagt aattagaccg attgctgcat ctttctcgaa aattactgag actctttcaa
     1201 aagtaag
//
LOCUS       DASJPH010000231         1193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJPH010000231 DASJPH010000000
VERSION     DASJPH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.239"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U3995_002983"
     CDS             <1..1076
                     /locus_tag="U3995_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1798249.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASJPH010000232         1186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJPH010000232 DASJPH010000000
VERSION     DASJPH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.240"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="U3995_002984"
     CDS             414..947
                     /locus_tag="U3995_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN1798250.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          382 a          218 c          219 g          367 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccg
//
LOCUS       DASJPH010000233         1184 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJPH010000233 DASJPH010000000
VERSION     DASJPH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.241"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(312..797)
                     /locus_tag="U3995_002985"
     CDS             complement(312..797)
                     /locus_tag="U3995_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798251.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="U3995_002986"
     CDS             complement(936..1151)
                     /locus_tag="U3995_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1798252.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
BASE COUNT          442 a          200 c          189 g          353 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aatt
//
LOCUS       DASJPH010000234         1174 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJPH010000234 DASJPH010000000
VERSION     DASJPH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.242"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..849
                     /locus_tag="U3995_002987"
     CDS             <1..849
                     /locus_tag="U3995_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1798253.1"
                     /translation="LKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKS
                     QNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEA
                     KVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRN
                     KLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLV
                     LADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMES
                     IYSRDH"
     gene            882..>1174
                     /locus_tag="U3995_002988"
     CDS             882..>1174
                     /locus_tag="U3995_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1798254.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
BASE COUNT          455 a          129 c          216 g          374 t
ORIGIN      
        1 ctaaaaaaag aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat
       61 ttatggttag ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa
      121 gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg
      181 ttacaaaatg caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa
      241 aaccacccct ttgagatagt tcataatggt attgatttag agctttatag taaaaattca
      301 gaagctaagg ttaataagag aaaagaattg gatatctcaa tgagtacttt tgttgtggga
      361 aacattggac gattagagaa acaaaaaaat caatcttatt tgttagagat ttttttagaa
      421 atactaaaaa tgaattctga ttcttacttg attttagtag gtgaagggtc acttagaaat
      481 aaactgaaag ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa
      541 agaaacgata ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat
      601 gaaggtctgc caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca
      661 gatactattt ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa
      721 acaccacaag aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa
      781 gaactaaaga gttatgatgt ttcgtacact actgaacaaa tggagagtat atatagtaga
      841 gatcattaaa aatgttcaag atattttagg agtgattatt tttgaaaata tgttatttga
      901 tattggcata caatgatatt ggcaacttaa agagattggt caatcgattg aacgaaaatg
      961 cagtttttat tattcatata gataaaaaaa ctgatataga accatttatt gaagcattta
     1021 aatcatatga taatgttgaa tttttatgta ataaaaaccg tgttaaaatt tattggggtg
     1081 gtttttccat tgtacaagca gaaatcaatt tagtaaagag agcattacaa aatgagaaat
     1141 atttaaaata tgtattatta tctggtgctg atta
//
LOCUS       DASJPH010000235         1095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJPH010000235 DASJPH010000000
VERSION     DASJPH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.244"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(210..283)
                     /locus_tag="U3995_002989"
     tRNA            complement(210..283)
                     /locus_tag="U3995_002989"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Ile,seq:cat)
     gene            complement(838..1065)
                     /locus_tag="U3995_002990"
     CDS             complement(838..1065)
                     /locus_tag="U3995_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798255.1"
                     /translation="MGFRTKRSTSSEKKWVYSQTIFYGGVISISLLSSTLYSFNVIDV
                     SMSNFISIIGILISAIITQLLLVFEEKSKNN"
BASE COUNT          409 a          179 c          166 g          341 t
ORIGIN      
        1 cacaatataa cccaaatgtc tttccattta aaaatcaaag taaaactttt caaatataca
       61 gaagtataac tatgtgaaac atcctttcat taatccataa aaggatacat aaaaaagcca
      121 ctcatttgag tggcaatgaa gaaaagcttt agcttgtata atactcttca aaaaattcta
      181 acacagaacg attcaattgg ctacattaat gtaccctgta ggactcgaac ctacgaccgg
      241 acggttatga gccgtctgct ctgaccaact gagctaaggg tagtggttgt tgccacataa
      301 agccataaac aatcaaccag tagaatgcgt ggcaacaaac ctgttatcgc atatcttgga
      361 gtgtgactat ttatgagtga tagtgaagat atgcgtctcc atatatgtat ggttgagaca
      421 gataaacatg tatgaaaaac gttgttaaat caacgttttt ttattttggt caaggagaga
      481 aaagtagaga aatacaaaat tttttgcatt gattttgcat tcaaaaaaac attcaaacat
      541 ttagaaatcc ccaatacgta ttcaggaaaa tctattttaa ggtttacaaa aagaactttt
      601 gttcgtacac tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg
      661 tttaagcatg ttccttcaaa taagtttttt aattataaac tctagagaaa ttaattcatc
      721 ctaacataaa ataattgttc actcaaaagt ttttctagaa caattaaaac agcaggcttc
      781 acattcgcta tacatttgta agaagtaata attcctgtac taaaaggcat aattgcttta
      841 attatttttt gacttctctt caaacacaag aagtagttgt gtaataattg cagaaataag
      901 gattccaata atagatataa aatttgacat ggaaacgtct ataacattaa aagaataaag
      961 tgttgaagaa agtaaactga ttgaaataac ccctccgtaa aaaatagtct gtgaatatac
     1021 ccactttttt tcagaagacg tacttcgttt ggttctaaaa cctatccctg catttctact
     1081 cttatcaaaa aacca
//
LOCUS       DASJPH010000236         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJPH010000236 DASJPH010000000
VERSION     DASJPH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.245"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U3995_002991"
     rRNA            <1..>1073
                     /locus_tag="U3995_002991"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASJPH010000237         1067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJPH010000237 DASJPH010000000
VERSION     DASJPH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.246"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="U3995_002992"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="U3995_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1798256.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DASJPH010000238         1066 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJPH010000238 DASJPH010000000
VERSION     DASJPH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.247"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U3995_002993"
     CDS             <1..969
                     /locus_tag="U3995_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1798257.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          367 a          184 c          214 g          301 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacc
//
LOCUS       DASJPH010000239         1062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJPH010000239 DASJPH010000000
VERSION     DASJPH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.248"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="U3995_002994"
     CDS             complement(37..993)
                     /locus_tag="U3995_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798258.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DASJPH010000240         1059 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJPH010000240 DASJPH010000000
VERSION     DASJPH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.249"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..457)
                     /locus_tag="U3995_002995"
     CDS             complement(5..457)
                     /locus_tag="U3995_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798259.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(464..1012)
                     /locus_tag="U3995_002996"
     CDS             complement(464..1012)
                     /locus_tag="U3995_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1798260.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          371 a          208 c          103 g          377 t
ORIGIN      
        1 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
       61 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      121 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      181 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      241 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      301 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      361 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      421 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      481 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      541 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      601 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      661 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
      721 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
      781 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
      841 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
      901 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
      961 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1021 ctccataaaa aataagttac tttttaaata tcttgttta
//
LOCUS       DASJPH010000241         1059 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJPH010000241 DASJPH010000000
VERSION     DASJPH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.250"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..988
                     /locus_tag="U3995_002997"
                     /pseudo
     CDS             1..988
                     /locus_tag="U3995_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          370 a          193 c          214 g          282 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagc
//
LOCUS       DASJPH010000242         1045 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJPH010000242 DASJPH010000000
VERSION     DASJPH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.251"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(108..908)
                     /locus_tag="U3995_002998"
     CDS             complement(108..908)
                     /locus_tag="U3995_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1798261.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
BASE COUNT          284 a          245 c          147 g          369 t
ORIGIN      
        1 ttgatttctt tttgcacttc ctggattgaa actttcttgt gagtgactag taatttcaat
       61 aattggtacc atctgtttac tagcgtcacg tgcgtccccc ctatccttta tcctcttgtt
      121 ttacctcctg aaatattagt cgtgactcca gtgatataac ttgatctttc tgaaagataa
      181 tagcagacta gatccgccac ttcttgcagc ttaccactcc ttcctagagg gatcgtactt
      241 gttcgactgt aaccgtttct cagctgttcg acgcttatac ctctcgtata agaaagagct
      301 tcttcatatt cttgtgttct caaaccggtt tcttccagta tccctggtgc aacacctacc
      361 acacgaacat tcctctttcc taactctttt gcccaagaac gagtaaaact gttcatcgct
      421 gcttttgtag cagcataaca gctctgtcct tctgatcctt ccaatcctga ttcagaagaa
      481 agattcacga tcactcctga tcccttttgc accaagattc ttccaactgc ttggctcatc
      541 aaatatacac ttttttgatt gacagcgatc atcttatcaa aaacttgttc tgataattca
      601 tacttgcttt ttggatgatt tctatctatt agaagagaag gaatatttat tcctgcatta
      661 ttgactagac catcgatcgt cttgaaatgt ttcattactt tatatatccc ttcttctatg
      721 tccgttttag aagacacatc gacttttaaa gatagtaggt tttcatgctg tatacggcat
      781 tcagttacat caaaattcgc tacttgtgca ttctgcttca acaaattttc gacgatactt
      841 cttccaattc ctgaggaacc tccagttaca attactactt ttcctgcaat atccaaccaa
      901 tccgacatat gtgatcctct ccttcttttt tcttacaaat ccagtataaa agctcactaa
      961 aaaaaattaa gacatgaaat ttacagcttc agctaaaaaa tgtgtactaa tggaagagca
     1021 aaaaaaattt tcatcttttt gcata
//
LOCUS       DASJPH010000243         1031 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJPH010000243 DASJPH010000000
VERSION     DASJPH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.252"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            120..>1031
                     /locus_tag="U3995_002999"
     CDS             120..>1031
                     /locus_tag="U3995_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798262.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          351 a          127 c          139 g          414 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt a
//
LOCUS       DASJPH010000244         1001 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJPH010000244 DASJPH010000000
VERSION     DASJPH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.253"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1001
                     /locus_tag="U3995_003000"
                     /pseudo
     CDS             369..1001
                     /locus_tag="U3995_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DASJPH010000245          968 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJPH010000245 DASJPH010000000
VERSION     DASJPH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.254"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(201..600)
                     /locus_tag="U3995_003001"
                     /pseudo
     CDS             complement(201..600)
                     /locus_tag="U3995_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(597..833)
                     /locus_tag="U3995_003002"
     CDS             complement(597..833)
                     /locus_tag="U3995_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1798263.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          310 a          168 c          133 g          357 t
ORIGIN      
        1 taaaaaatca gcccactagt tctaaaccac tatttcctac tattcactct tttgtattcc
       61 ttttaaatca attatttcaa aattaatcca tattttccat atttttatga aaaaactatt
      121 ttgtatgatt aaacataaaa aaatcagcat attccttaat gacatgctga tctaattgag
      181 aaataaagtt tttaattcgt tcattttatt gcggtttgtc gtaaataact ggttatcttg
      241 gactttagtg aatcgaaatc caatgaacta gtggtaatca ttatgataaa atcaacagct
      301 tcttgacgag aaaaagcaac tggacaatta ttcaattgaa gaaacaacaa catggcaaca
      361 agagcagttc gtttattgcc attatgaaaa ggatgttttt tagctaaatt aattgctaaa
      421 atcgccgctt tttcatagat ttcaggatag agttcttgat tgaatactgc ttgtgaaggc
      481 tgtttgacgg ccatatctaa tgcatttgca tcttttacac cgatcatttc gcctggcgag
      541 tgacggtaaa ataactttcg cgttgatttt gataatttct ttagtcgaaa gatacctcat
      601 aaatcaacca aatcttttaa cgcgtcttca tattgattca tgatcgaatc aactaatgaa
      661 tcaatatcag gttcttcttg tgctttgatc attcgtacag aaccatcaga ttgaaaaaca
      721 taactgattg cgtcacctgt ctgcacaccc atgaattctt ttacttctgg tgggatagtt
      781 gttacatcgc tattgccaga ttttctaatt ttatactttt tcattttttt catatttcgc
      841 caccctctat accattctca ttctatagta agtacattat atccaagtat atacttttaa
      901 tcaatgtata tacttttatt ttgctttttc ttacgaaaaa atgacaaaga aaaaagtttg
      961 tgacatga
//
LOCUS       DASJPH010000246          961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJPH010000246 DASJPH010000000
VERSION     DASJPH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.255"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..878
                     /locus_tag="U3995_003003"
     CDS             <1..878
                     /locus_tag="U3995_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1798264.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          301 a          212 c          192 g          256 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 a
//
LOCUS       DASJPH010000247          934 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJPH010000247 DASJPH010000000
VERSION     DASJPH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.256"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..>934
                     /locus_tag="U3995_003004"
     CDS             4..>934
                     /locus_tag="U3995_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1798265.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDK"
BASE COUNT          318 a          172 c          196 g          248 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaac
//
LOCUS       DASJPH010000248          930 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJPH010000248 DASJPH010000000
VERSION     DASJPH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.257"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..801
                     /gene="truA"
                     /locus_tag="U3995_003005"
     CDS             55..801
                     /gene="truA"
                     /locus_tag="U3995_003005"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1798266.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT          324 a          153 c          208 g          245 t
ORIGIN      
        1 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
       61 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
      121 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
      181 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
      241 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
      301 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
      361 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
      421 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
      481 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
      541 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
      601 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
      661 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
      721 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
      781 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
      841 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
      901 tacttctgga acaccgttta aggaagaggt
//
LOCUS       DASJPH010000249          910 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJPH010000249 DASJPH010000000
VERSION     DASJPH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.258"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="U3995_003006"
     CDS             <1..355
                     /locus_tag="U3995_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798267.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="U3995_003007"
                     /pseudo
     CDS             615..910
                     /locus_tag="U3995_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DASJPH010000250          906 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJPH010000250 DASJPH010000000
VERSION     DASJPH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.259"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..767
                     /locus_tag="U3995_003008"
     CDS             <1..767
                     /locus_tag="U3995_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798268.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DASJPH010000251          893 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJPH010000251 DASJPH010000000
VERSION     DASJPH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.260"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            252..875
                     /locus_tag="U3995_003009"
     CDS             252..875
                     /locus_tag="U3995_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798269.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          328 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aag
//
LOCUS       DASJPH010000252          810 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJPH010000252 DASJPH010000000
VERSION     DASJPH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.261"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..799
                     /locus_tag="U3995_003010"
     CDS             <1..799
                     /locus_tag="U3995_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798270.1"
                     /translation="LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNH
                     TPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSN
                     SIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTH
                     FMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGIN
                     KVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT          272 a          130 c          103 g          305 t
ORIGIN      
        1 cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt
       61 atttattttc tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca
      121 actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc
      181 ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa
      241 taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt
      301 aagcaattct atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt
      361 tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc
      421 tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca
      481 ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa atactgtctt
      541 gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca
      601 aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt
      661 cgttattact tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac
      721 tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga
      781 agtacaacat tctcactaga tttttattta
//
LOCUS       DASJPH010000253          807 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJPH010000253 DASJPH010000000
VERSION     DASJPH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.262"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="U3995_003011"
     CDS             132..344
                     /locus_tag="U3995_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798271.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>807
                     /locus_tag="U3995_003012"
     CDS             506..>807
                     /locus_tag="U3995_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1798272.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSST"
BASE COUNT          297 a          112 c          133 g          265 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccga
//
LOCUS       DASJPH010000254          782 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJPH010000254 DASJPH010000000
VERSION     DASJPH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.263"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..607)
                     /locus_tag="U3995_003013"
     CDS             complement(<1..607)
                     /locus_tag="U3995_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1798273.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINS"
BASE COUNT          268 a          131 c           81 g          302 t
ORIGIN      
        1 tactgttaat ttcaagattc gaatatttac caaactgttt ttttactgat ttagggaaac
       61 caaataggtt tataatataa tccaattcat gtatacagtc taattctact cctcccccca
      121 tagatttaaa ggcagaataa ttatttctat aatcagtacc ttttctccaa tcaggaagat
      181 aagtagaaca aatagctctt acagaatata cttttttact ttgaatacta ttacgaacaa
      241 cttccatgat atttttatac ctcagtggag cagcaacata aatatttttg taatcataat
      301 tatattgagt aagatcaata ctctcagaaa aagcaggttt ttctaaaaat atgtaatcaa
      361 ctttttcaac aattacattt agtgtttcca gatgcttaaa agtcggattt gtaataaaag
      421 ctatatcata atgtgtatcc aaatcgtctc tattttgaat atacgttatt tcttcaaact
      481 ctttttttcc actgctacta taagctgtta tatcaaaatt aatagaattc ttttttccat
      541 agcttattag attttttaga tgtctctttc caattgaccc taacccaaaa aaaattactt
      601 tcatcattaa tttacctcaa tgtcgttaat taagttaata atatacttat ctttttcaaa
      661 agtatattgg ggttccattt tcttttccaa aagcgtgaaa aattctttaa tctcctctaa
      721 atatgcatct tcaataatag tatgtgaata attattatca tgcataacct catcgtacaa
      781 tt
//
LOCUS       DASJPH010000255          777 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJPH010000255 DASJPH010000000
VERSION     DASJPH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.264"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..211)
                     /locus_tag="U3995_003014"
     tRNA            complement(127..211)
                     /locus_tag="U3995_003014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Leu,seq:caa)
     gene            complement(232..302)
                     /locus_tag="U3995_003015"
     tRNA            complement(232..302)
                     /locus_tag="U3995_003015"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Cys,seq:gca)
     gene            complement(348..419)
                     /locus_tag="U3995_003016"
     tRNA            complement(348..419)
                     /locus_tag="U3995_003016"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Gln,seq:ttg)
     gene            complement(431..503)
                     /locus_tag="U3995_003017"
     tRNA            complement(431..503)
                     /locus_tag="U3995_003017"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:His,seq:gtg)
     gene            complement(506..579)
                     /locus_tag="U3995_003018"
     tRNA            complement(506..579)
                     /locus_tag="U3995_003018"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(545..547),aa:Trp,seq:cca)
     gene            complement(586..666)
                     /locus_tag="U3995_003019"
     tRNA            complement(586..666)
                     /locus_tag="U3995_003019"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Tyr,seq:gta)
     gene            complement(668..743)
                     /locus_tag="U3995_003020"
     tRNA            complement(668..743)
                     /locus_tag="U3995_003020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:Phe,seq:gaa)
BASE COUNT          226 a          200 c          174 g          177 t
ORIGIN      
        1 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
       61 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
      121 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
      181 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
      241 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
      301 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
      361 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
      421 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
      481 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
      541 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
      601 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
      661 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
      721 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacg
//
LOCUS       DASJPH010000256          760 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJPH010000256 DASJPH010000000
VERSION     DASJPH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.265"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(101..424)
                     /locus_tag="U3995_003021"
     CDS             complement(101..424)
                     /locus_tag="U3995_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798274.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            611..684
                     /locus_tag="U3995_003022"
     tRNA            611..684
                     /locus_tag="U3995_003022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Ile,seq:cat)
BASE COUNT          312 a          109 c          128 g          211 t
ORIGIN      
        1 aaatgctagg aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt
       61 ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg
      121 gattgatatc taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg
      181 aaagaatgat gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac
      241 gggcatatct ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac
      301 cttgaaaaat cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat
      361 aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactccta
      421 acattgagaa taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt
      481 aacaaaatag ttatgacaca agtaaaagaa ataagaataa caatattatt ttacttatat
      541 tttgaaactt gtgagagttt gtcattcgtg ctatagttga aaagtaacaa aaataaattt
      601 tatttatgat gtacctttag ctcagttggt tagagcagac ggctcataac cgtccggtcg
      661 taggttcgag tcctacaagg tacataagag gttgtgaaaa agctgccctg catcaagtaa
      721 taagaacagc caagtaaaaa cagcttctta tgtttttact
//
LOCUS       DASJPH010000257          757 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJPH010000257 DASJPH010000000
VERSION     DASJPH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.266"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(16..528)
                     /locus_tag="U3995_003023"
     CDS             complement(16..528)
                     /locus_tag="U3995_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798275.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..720)
                     /locus_tag="U3995_003024"
     CDS             complement(535..720)
                     /locus_tag="U3995_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEN1798276.1"
                     /translation="MEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNN
                     YQFGNLSYEMISKVLSL"
BASE COUNT          258 a          110 c           71 g          318 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggta
//
LOCUS       DASJPH010000258          751 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJPH010000258 DASJPH010000000
VERSION     DASJPH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.267"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>751
                     /locus_tag="U3995_003025"
     CDS             10..>751
                     /locus_tag="U3995_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798277.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          254 a          131 c          149 g          217 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta g
//
LOCUS       DASJPH010000259          731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJPH010000259 DASJPH010000000
VERSION     DASJPH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.268"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..524)
                     /locus_tag="U3995_003026"
     CDS             complement(324..524)
                     /locus_tag="U3995_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1798278.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DASJPH010000260          725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJPH010000260 DASJPH010000000
VERSION     DASJPH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.270"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>725
                     /locus_tag="U3995_003027"
     CDS             28..>725
                     /locus_tag="U3995_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1798279.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DASJPH010000261          719 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJPH010000261 DASJPH010000000
VERSION     DASJPH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.271"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(334..>719)
                     /locus_tag="U3995_003028"
     CDS             complement(334..>719)
                     /locus_tag="U3995_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEN1798280.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DASJPH010000262          670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJPH010000262 DASJPH010000000
VERSION     DASJPH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.272"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..417
                     /locus_tag="U3995_003029"
     CDS             154..417
                     /locus_tag="U3995_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1798281.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="U3995_003030"
     CDS             417..>670
                     /locus_tag="U3995_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1798282.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DASJPH010000263          662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJPH010000263 DASJPH010000000
VERSION     DASJPH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.273"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>662
                     /locus_tag="U3995_003031"
     CDS             <1..>662
                     /locus_tag="U3995_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798283.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIAD
                     RLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLP
                     QI"
BASE COUNT          200 a          126 c          138 g          198 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga actttcttga
      301 atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct caacgagggt
      361 tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgccg
      481 atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact agagacatta
      601 gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tc
//
LOCUS       DASJPH010000264          656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJPH010000264 DASJPH010000000
VERSION     DASJPH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.274"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U3995_003032"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U3995_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1798284.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="U3995_003033"
     CDS             complement(481..>656)
                     /locus_tag="U3995_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798285.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DASJPH010000265          653 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJPH010000265 DASJPH010000000
VERSION     DASJPH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.275"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..541)
                     /locus_tag="U3995_003034"
     CDS             complement(<1..541)
                     /locus_tag="U3995_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1798286.1"
                     /translation="MKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSE
                     ISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISN
                     IGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFR
                     KQEVNKINQRKEFFKVSLDE"
BASE COUNT          185 a          115 c           68 g          285 t
ORIGIN      
        1 tttcgtctag tgaaactttg aaaaattctt ttctttgatt aattttatta acttcttgtt
       61 ttctaaaatg attatgcaat ttagtttcca aagacggagc gtcctctgaa aaaatcatcg
      121 catgaacgtc aaattcaaat ggaaccgatg cactactcaa ttcttttatt ctatccatag
      181 gttctaatct tctagtcata ccaattttat atatgtcttc accaaaagac ccaatattac
      241 taattacata cacataaccg gctctagtat ttaattctct ttgtaataca ttttcacgta
      301 taacttctaa ttcttttaat ttttcttcca gttcttgaat tttatttgca tataattctt
      361 tttccacatc tgcattagct ttttgcatat atgcgattaa tttggaaatt tcagaattga
      421 attgacgttg ttctttatca atacgtttct tttcacgatc aatttctcta cgaacttttt
      481 cctcttctat catctgttct tttattgctt tttgttgttc tttttcttcg gtaattttca
      541 ctaaataaga gtaatgaagt tctagttctt gaagtttaaa ctctaaaaga cccttcctta
      601 attcagcatt gtcattcttg aataaattat taattttatc aaatattttt atg
//
LOCUS       DASJPH010000266          630 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJPH010000266 DASJPH010000000
VERSION     DASJPH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.276"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>630
                     /locus_tag="U3995_003035"
     rRNA            <1..>630
                     /locus_tag="U3995_003035"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DASJPH010000267          600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJPH010000267 DASJPH010000000
VERSION     DASJPH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.277"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>600)
                     /locus_tag="U3995_003036"
     CDS             complement(<1..>600)
                     /locus_tag="U3995_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1798287.1"
                     /translation="VEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIF
                     RAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIG
                     MFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNK
                     WFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKV"
BASE COUNT          195 a           87 c           73 g          245 t
ORIGIN      
        1 aactttcaaa atttctttat caattctatt taatagttta tcagagtaat ccgatttaac
       61 ttttcttaca aataattttt cactttcttt tatttgatct atatcccttt catcaaacca
      121 tttgttcatg gaaggatcaa taatatgaat atttgctaat ttcgaagttt gtaaagtagt
      181 ttcttcaata tttttcaatt caagtggtaa atctggtcct ccaataacat ttttattttt
      241 gaacgaggaa ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat
      301 tttaaaattc aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca
      361 ttctttcgac aaagcccacc attgagaacc atgatacaaa tcaaatttat ggtgtctaat
      421 ttttggggga agtttaatca tcctcagaca acgattaatt atagctctaa aaattttaaa
      481 tgcagttgtg ttattcctat taattctagg ataatcatgt ttttgatata catctatatg
      541 tttataataa aattctttat gtttaatttg atctagatct attccacgaa taaactccac
//
LOCUS       DASJPH010000268          595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJPH010000268 DASJPH010000000
VERSION     DASJPH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.278"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..563)
                     /locus_tag="U3995_003037"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="U3995_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          168 a          109 c           78 g          240 t
ORIGIN      
        1 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
       61 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc taactttttt
      121 aaatatgcgt tctctgctct taaacgaata acttcttctt caagagactc atctttaagt
      181 tttttaggaa tgtttagctt ggaattcata ctaatttttc tgcccctttt ttggctctca
      241 agtgaagaag caccgccttc ttcatattgc tctatccatt tacttagagt tctatttgaa
      301 ccgatattaa attttttagc agtttcttgg atagaaagac catttgtttc catatattct
      361 ataacatcaa gtttaaattt tgtagtgtag cttttgccac ctccaaccaa gccttcccaa
      421 ccatgatagt tataaatcct tacccaatgt ctaaccagtg tacgatttat ttgatattta
      481 tgtgcaagat atttgtagcc gccttcgtta tttaaatagt ctgaaactac ttttttctta
      541 aaaacaaatg tatatttccg caaaaaaagc acccctttta attagatttc tagtc
//
LOCUS       DASJPH010000269          593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJPH010000269 DASJPH010000000
VERSION     DASJPH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.279"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..580
                     /locus_tag="U3995_003038"
     CDS             <1..580
                     /locus_tag="U3995_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1798288.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DASJPH010000270          537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJPH010000270 DASJPH010000000
VERSION     DASJPH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.280"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>537
                     /locus_tag="U3995_003039"
     CDS             <1..>537
                     /locus_tag="U3995_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1798289.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTT"
BASE COUNT          185 a           96 c          104 g          152 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccac
//
LOCUS       DASJPH010000271          528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJPH010000271 DASJPH010000000
VERSION     DASJPH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.281"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="U3995_003040"
     tRNA            complement(5..90)
                     /locus_tag="U3995_003040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U3995_003041"
     tRNA            complement(107..178)
                     /locus_tag="U3995_003041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U3995_003042"
     tRNA            complement(190..262)
                     /locus_tag="U3995_003042"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U3995_003043"
     tRNA            complement(280..361)
                     /locus_tag="U3995_003043"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U3995_003044"
     tRNA            complement(369..441)
                     /locus_tag="U3995_003044"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U3995_003045"
     tRNA            complement(445..517)
                     /locus_tag="U3995_003045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASJPH010000272          527 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJPH010000272 DASJPH010000000
VERSION     DASJPH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.282"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            65..514
                     /locus_tag="U3995_003046"
     CDS             65..514
                     /locus_tag="U3995_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798290.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
BASE COUNT          195 a           91 c          116 g          125 t
ORIGIN      
        1 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
       61 gaaaatgaca gatatcgtaa aaataaaaca aagcggtgta caggtttatc cacaaactca
      121 ttggaatgct atagaaggca aaccaacaac ggtgaaggga gataaaggcg atcctggcca
      181 agcagcaaca attaccatag gaaccgtttc tagtggttca accgcttctg ttacgaatgt
      241 aggaacatca tctgcagcta gatttaattt tgttttacca aagggagata aaggtgatcc
      301 tggaataaat gccacaacta cagctgtagc tacaactaca gcaaatgggt tgatgtcgtc
      361 cacagataaa actaagttgg atggaattgc agctggagca cagaaaaatc caggtaatgc
      421 tacaactacg acagcaggtt taatgtcagc aactgacaaa gctaaactcg atggattagc
      481 caatattaca tttgagaaag tggggacggt ttaatggctg atattgt
//
LOCUS       DASJPH010000273          526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJPH010000273 DASJPH010000000
VERSION     DASJPH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.283"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..354
                     /locus_tag="U3995_003047"
     CDS             <1..354
                     /locus_tag="U3995_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1798291.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="U3995_003048"
     CDS             355..>526
                     /locus_tag="U3995_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798292.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DASJPH010000274          517 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJPH010000274 DASJPH010000000
VERSION     DASJPH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.284"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            57..>517
                     /locus_tag="U3995_003049"
     CDS             57..>517
                     /locus_tag="U3995_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1798293.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DASJPH010000275          516 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJPH010000275 DASJPH010000000
VERSION     DASJPH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.285"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..336)
                     /locus_tag="U3995_003050"
     CDS             complement(34..336)
                     /locus_tag="U3995_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798294.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          176 a           92 c           71 g          177 t
ORIGIN      
        1 ccgttccggg ctaccttttt taggtagcct actttaatct ttataccttt ctggatcaac
       61 gaaagtatac ttcaaatagt cataacgccg atgatcgctc cgtgcgtctg gcacatcagt
      121 cacgatatca aacaaaaaat acacatcttt cttcattctg gtttttgttg ccggaatttt
      181 aaagtagttc ttattagagt aatagagatt gattaataag ctatcttcga ttgctaaaaa
      241 gaaaacttct gaatcccaca ccttataaaa atctttgaca aatctattcg aaggatcaaa
      301 tttaaaccat aattgtgttt ttccttccat caacataatg ttcacctcaa aaagagtata
      361 cgaactaatg ttctttttgt aaagtaggaa tatcattgat aatatggatg gaacatatat
      421 attaaaaagt gttctttttg aatgctattt tgaatgccat tttcattcat ttaacgaaaa
      481 tctgcgaaaa taaaaaatgc tcaaaccctt ataaaa
//
LOCUS       DASJPH010000276          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJPH010000276 DASJPH010000000
VERSION     DASJPH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.286"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U3995_003051"
     CDS             complement(<1..132)
                     /locus_tag="U3995_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1798295.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DASJPH010000277          512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJPH010000277 DASJPH010000000
VERSION     DASJPH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.287"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..343
                     /locus_tag="U3995_003052"
     CDS             <1..343
                     /locus_tag="U3995_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1798296.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DASJPH010000278          510 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJPH010000278 DASJPH010000000
VERSION     DASJPH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.288"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..484
                     /locus_tag="U3995_003053"
     CDS             <1..484
                     /locus_tag="U3995_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN1798297.1"
                     /translation="FELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKN
                     GERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKIT
                     AYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM
                     "
BASE COUNT          191 a           91 c           84 g          144 t
ORIGIN      
        1 tttcgaactg ttgggtgaat ccagcagtag tttttctaca ctttgctttc cagaagccac
       61 gtccaatcat tctagttctt ttatacagga aaagaaatta agagaaaaaa tcgatacaat
      121 tttttcaaaa aatggggaaa gaaaccaaat cagtcaatac cttagtcaat tgatccaaca
      181 cttactacaa acagaaacga aagagcagtt actcatccat ttgcagataa ccaaacagtt
      241 tacaggaaat tatcatcttc aaagccattt gcagcagtta tttcgcaaag atgctgttaa
      301 aattaccgca tattcagaac atgctgattt gatcattaca gatcagcatg agaaaatttc
      361 agatgaaacg gagactttct atctggatac aacgcacaat acagtgttat ttggcaagtt
      421 aataaaagcg gttcatgaac tgtatatgaa aaaaggttat gagaacaaaa caaagaggat
      481 gtgacaaaat tcttgtcaca tcctcttatt
//
LOCUS       DASJPH010000279          507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJPH010000279 DASJPH010000000
VERSION     DASJPH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.289"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>507
                     /locus_tag="U3995_003054"
     CDS             52..>507
                     /locus_tag="U3995_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1798298.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYR"
BASE COUNT          154 a          101 c           97 g          155 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgc
//
LOCUS       DASJPH010000280          490 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJPH010000280 DASJPH010000000
VERSION     DASJPH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.290"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          161 a           96 c           57 g          176 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatgaatttt
//
LOCUS       DASJPH010000281          487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJPH010000281 DASJPH010000000
VERSION     DASJPH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.291"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>487)
                     /locus_tag="U3995_003055"
     CDS             complement(<1..>487)
                     /locus_tag="U3995_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEN1798299.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DASJPH010000282          468 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJPH010000282 DASJPH010000000
VERSION     DASJPH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.292"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>468
                     /locus_tag="U3995_003056"
     CDS             <1..>468
                     /locus_tag="U3995_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1798300.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DASJPH010000283          457 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJPH010000283 DASJPH010000000
VERSION     DASJPH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.293"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>457
                     /locus_tag="U3995_003057"
     CDS             <1..>457
                     /locus_tag="U3995_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1798301.1"
                     /translation="KNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKR
                     DTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLI
                     EQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          155 a           91 c           92 g          119 t
ORIGIN      
        1 gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca aaattaaggg
       61 acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag atatctggtt
      121 acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc ataaacagtt
      181 tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg cctttagaaa
      241 gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt atcttaacaa
      301 tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc aaagtctccg
      361 tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg
      421 aagaaatgga acgctcttcg gcttttcggt gtctact
//
LOCUS       DASJPH010000284          453 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJPH010000284 DASJPH010000000
VERSION     DASJPH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.294"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>453
                     /locus_tag="U3995_003058"
     CDS             <1..>453
                     /locus_tag="U3995_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798302.1"
                     /translation="TSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIG
                     TIGTTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYF
                     GDLDIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT          172 a           67 c          100 g          114 t
ORIGIN      
        1 aacttccttt aatgcttatg gtgatgtgtt aaaacttgta aaaggcacgc ttcaaatttg
       61 gaatgaaaac aaaaaaatca tggagctaac caaaaaaggt atggaattct ggaatagaaa
      121 agaatccatt ggaacgatcg gaacaacaga ttcagcagga aatccatttc caaatgctgt
      181 aacacctaca cctcttgagg aaaattcttt ggtaatacgt acaaatggcg atggaaaata
      241 cattttaatt tctccaactg cagaaaaagg atttgtgtta ttaggaaacg gaaaagcata
      301 ttatttcggt gatttagata ttcagggaaa acttactgtt agaggtaaag aagtgatacc
      361 aggacaaaat ggcggtccat ctggcggagg agaaacacct ggtgggtatc ctgatgaatt
      421 aaaaacagat gcagaaaaga gagcttggag aat
//
LOCUS       DASJPH010000285          439 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJPH010000285 DASJPH010000000
VERSION     DASJPH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.295"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..422
                     /locus_tag="U3995_003059"
     CDS             93..422
                     /locus_tag="U3995_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1798303.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          169 a           52 c           79 g          139 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaac
//
LOCUS       DASJPH010000286          421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJPH010000286 DASJPH010000000
VERSION     DASJPH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.296"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..355)
                     /locus_tag="U3995_003060"
     CDS             complement(170..355)
                     /locus_tag="U3995_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEN1798304.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DASJPH010000287          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJPH010000287 DASJPH010000000
VERSION     DASJPH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.297"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U3995_003061"
     CDS             <1..390
                     /locus_tag="U3995_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798305.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DASJPH010000288          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJPH010000288 DASJPH010000000
VERSION     DASJPH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.298"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="U3995_003062"
     CDS             complement(<1..339)
                     /locus_tag="U3995_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1798306.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASJPH010000289          404 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJPH010000289 DASJPH010000000
VERSION     DASJPH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.299"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>404
                     /locus_tag="U3995_003063"
     CDS             <1..>404
                     /locus_tag="U3995_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798307.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DASJPH010000290          381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJPH010000290 DASJPH010000000
VERSION     DASJPH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.300"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="U3995_003064"
     tRNA            complement(21..94)
                     /locus_tag="U3995_003064"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="U3995_003065"
     tRNA            complement(108..181)
                     /locus_tag="U3995_003065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="U3995_003066"
     tRNA            complement(209..281)
                     /locus_tag="U3995_003066"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="U3995_003067"
     tRNA            complement(301..374)
                     /locus_tag="U3995_003067"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DASJPH010000291          377 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJPH010000291 DASJPH010000000
VERSION     DASJPH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.301"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>377
                     /locus_tag="U3995_003068"
     CDS             <1..>377
                     /locus_tag="U3995_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798308.1"
                     /translation="FLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGE
                     FKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLT
                     EEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          137 a           66 c           62 g          112 t
ORIGIN      
        1 tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt
       61 tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat
      121 cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg
      181 gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg
      241 atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt
      301 ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt
      361 tttatggacg ctactta
//
LOCUS       DASJPH010000292          371 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJPH010000292 DASJPH010000000
VERSION     DASJPH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.302"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U3995_003069"
     tRNA            complement(20..91)
                     /locus_tag="U3995_003069"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U3995_003070"
     tRNA            complement(113..200)
                     /locus_tag="U3995_003070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U3995_003071"
     tRNA            complement(225..298)
                     /locus_tag="U3995_003071"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
BASE COUNT          110 a           96 c           86 g           79 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta a
//
LOCUS       DASJPH010000293          366 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJPH010000293 DASJPH010000000
VERSION     DASJPH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.303"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>366)
                     /locus_tag="U3995_003072"
     CDS             complement(<1..>366)
                     /locus_tag="U3995_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798309.1"
                     /translation="LDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFE
                     IERSQTAVVELTKENRLTPG"
BASE COUNT           69 a          107 c           73 g          117 t
ORIGIN      
        1 gcctggtgta agtcgatttt ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat
       61 ctcaaactcg attggtgtcg catccaattc ataccccgtt ggcgcttggg tttctaccag
      121 ctgatacact cctggtgcta agccgtcaat cgccagtttt ccatctgctc ctgtggttag
      181 acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc cttgcaggac
      241 ttccccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa gtcggtttgc
      301 tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga tcggtgtcgc
      361 atctaa
//
LOCUS       DASJPH010000294          362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJPH010000294 DASJPH010000000
VERSION     DASJPH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.304"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>362)
                     /locus_tag="U3995_003073"
     rRNA            complement(<1..>362)
                     /locus_tag="U3995_003073"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DASJPH010000295          362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJPH010000295 DASJPH010000000
VERSION     DASJPH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.305"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..261
                     /locus_tag="U3995_003074"
     CDS             22..261
                     /locus_tag="U3995_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1798310.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            310..>362
                     /locus_tag="U3995_003075"
     CDS             310..>362
                     /locus_tag="U3995_003075"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1798311.1"
                     /translation="MLVFQMRNVDKTSTVLK"
BASE COUNT          151 a           38 c           80 g           93 t
ORIGIN      
        1 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga
       61 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
      121 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
      181 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
      241 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
      301 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      361 ca
//
LOCUS       DASJPH010000296          344 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJPH010000296 DASJPH010000000
VERSION     DASJPH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.306"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>344
                     /locus_tag="U3995_003076"
     CDS             22..>344
                     /locus_tag="U3995_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798312.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTL"
BASE COUNT          121 a           68 c           68 g           87 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccct
//
LOCUS       DASJPH010000297          340 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJPH010000297 DASJPH010000000
VERSION     DASJPH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.307"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..268
                     /locus_tag="U3995_003077"
                     /pseudo
     CDS             1..268
                     /locus_tag="U3995_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          122 a           46 c           64 g          108 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc
//
LOCUS       DASJPH010000298          335 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJPH010000298 DASJPH010000000
VERSION     DASJPH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.308"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>335
                     /locus_tag="U3995_003078"
     CDS             <1..>335
                     /locus_tag="U3995_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN1798313.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          125 a           53 c           77 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgag
//
LOCUS       DASJPH010000299          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJPH010000299 DASJPH010000000
VERSION     DASJPH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.309"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U3995_003079"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U3995_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN1798314.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DASJPH010000300          302 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJPH010000300 DASJPH010000000
VERSION     DASJPH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.310"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          107 a           33 c           48 g          114 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 at
//
LOCUS       DASJPH010000301          285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJPH010000301 DASJPH010000000
VERSION     DASJPH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.311"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..285
                     /locus_tag="U3995_003080"
                     /pseudo
     CDS             1..285
                     /locus_tag="U3995_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          110 a           58 c           56 g           61 t
ORIGIN      
        1 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta
       61 tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt
      121 caaagaaaga gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa
      181 taagcaactc gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa
      241 agacaagtaa ttgatgccaa caagcataaa tattccatat cagcg
//
LOCUS       DASJPH010000302          260 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJPH010000302 DASJPH010000000
VERSION     DASJPH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.312"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..208)
                     /locus_tag="U3995_003081"
     CDS             complement(<1..208)
                     /locus_tag="U3995_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798315.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          102 a           42 c           26 g           90 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat
//
LOCUS       DASJPH010000303          256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJPH010000303 DASJPH010000000
VERSION     DASJPH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.313"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..>256)
                     /locus_tag="U3995_003082"
     CDS             complement(26..>256)
                     /locus_tag="U3995_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798316.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           73 a           38 c           44 g          101 t
ORIGIN      
        1 tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa
       61 tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga
      121 tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa
      181 taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt
      241 caggaagatt ttttaa
//
LOCUS       DASJPH010000304          255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJPH010000304 DASJPH010000000
VERSION     DASJPH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.314"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..239
                     /locus_tag="U3995_003083"
     CDS             <1..239
                     /locus_tag="U3995_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEN1798317.1"
                     /translation="GNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGK
                     ETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
BASE COUNT          110 a           32 c           43 g           70 t
ORIGIN      
        1 gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
       61 agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat
      121 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca
      181 atgccgttgt aaaaattggg gaaaagggat atattgattt tatctctttt tcaaaataaa
      241 cataaaagcc cttta
//
LOCUS       DASJPH010000305          252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJPH010000305 DASJPH010000000
VERSION     DASJPH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.315"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..>252)
                     /locus_tag="U3995_003084"
     CDS             complement(32..>252)
                     /locus_tag="U3995_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1798318.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DASJPH010000306          239 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJPH010000306 DASJPH010000000
VERSION     DASJPH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.316"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..222
                     /locus_tag="U3995_003085"
     CDS             16..222
                     /locus_tag="U3995_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1798319.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           84 a           24 c           51 g           80 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttct
//
LOCUS       DASJPH010000307          233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJPH010000307 DASJPH010000000
VERSION     DASJPH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.317"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DASJPH010000308          232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJPH010000308 DASJPH010000000
VERSION     DASJPH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.318"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>232)
                     /locus_tag="U3995_003086"
     CDS             complement(<1..>232)
                     /locus_tag="U3995_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798320.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DASJPH010000309          224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJPH010000309 DASJPH010000000
VERSION     DASJPH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.319"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="U3995_003087"
     CDS             <1..>224
                     /locus_tag="U3995_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN1798321.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DASJPH010000310          220 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJPH010000310 DASJPH010000000
VERSION     DASJPH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.320"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..175)
                     /locus_tag="U3995_003088"
     rRNA            complement(<1..175)
                     /locus_tag="U3995_003088"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DASJPH010000311          207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJPH010000311 DASJPH010000000
VERSION     DASJPH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.321"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>207
                     /locus_tag="U3995_003089"
     CDS             <1..>207
                     /locus_tag="U3995_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1798322.1"
                     /translation="AVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDID
                     IYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           74 a           37 c           36 g           60 t
ORIGIN      
        1 ggcagttgat attgaaaatt cgattaatga atggcttcaa tccgtaccca aaaatctttt
       61 caaatcaatt acctttgatt gtggaaaaga attctctaat tggaaaagta ttagtaatac
      121 gaatgatatt gatatttatt tcgcagaccc aggaacgcct tcccaacggg gattaaatga
      181 acattcaaac ggactcctcc gaaaaga
//
LOCUS       DASJPH010000312          206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJPH010000312 DASJPH010000000
VERSION     DASJPH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.denovo.322"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>206)
                     /locus_tag="U3995_003090"
     CDS             complement(<1..>206)
                     /locus_tag="U3995_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1798323.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DASJPH010000313        10793 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.096, whole genome shotgun sequence.
ACCESSION   DASJPH010000313 DASJPH010000000
VERSION     DASJPH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.096"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..1171
                     /locus_tag="U3995_003091"
     CDS             260..1171
                     /locus_tag="U3995_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN1798324.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="U3995_003092"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="U3995_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1798325.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="U3995_003093"
     CDS             2328..2612
                     /locus_tag="U3995_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798326.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="U3995_003094"
     CDS             2978..3277
                     /locus_tag="U3995_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798327.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="U3995_003095"
     CDS             3320..4810
                     /locus_tag="U3995_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1798328.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5158..5328
                     /locus_tag="U3995_003096"
     CDS             5158..5328
                     /locus_tag="U3995_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1798329.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5342..5959
                     /locus_tag="U3995_003097"
     CDS             5342..5959
                     /locus_tag="U3995_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1798330.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5959..6897
                     /locus_tag="U3995_003098"
                     /pseudo
     CDS             5959..6897
                     /locus_tag="U3995_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(7590..8237)
                     /locus_tag="U3995_003099"
     CDS             complement(7590..8237)
                     /locus_tag="U3995_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN1798331.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(8367..9305)
                     /locus_tag="U3995_003100"
     CDS             complement(8367..9305)
                     /locus_tag="U3995_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1798332.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            9796..10793
                     /locus_tag="U3995_003101"
                     /pseudo
     CDS             9796..10793
                     /locus_tag="U3995_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT         3909 a         1671 c         1878 g         3335 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa
     4921 ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa
     4981 gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt
     5041 ttaattttct atcttttata ggtcattaga gtatacctat ttgtcctaat atgattttag
     5101 cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag gagtaaaatg
     5161 aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt taatgagcaa
     5221 caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca atttgaaatt
     5281 gaaaaagtag atattgctga ttggttattg caaccgagag aattttagag gttggttgaa
     5341 aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag atcggcaatt
     5401 acaagcgtta cagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga
     5461 acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatattg ttattgttac
     5521 tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga atcaaattca
     5581 aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta ttgaagatga
     5641 aaatttaagg cgtttgatta atagccttgt cattgaattg tacaagtatc aagcagaatc
     5701 agaacgaaaa aaaattaagg aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg
     5761 caaattcaaa ggtcgtcagc ataaatttaa agaaaatgat ccacgtttaa agcatgcttt
     5821 cgatttgttt ttgaatggtt tatccgataa agaagttgaa gaacaaactg gaatcaatcg
     5881 ccgaacgttt agaaggtatc gagcaagata taacgtgaca gtcgatcaaa gaaaaaacaa
     5941 tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag ccaggcatta
     6001 gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac
     6061 ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca
     6121 gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt
     6181 cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca
     6241 gaacttctca aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa
     6301 aatatcgcct ggtcgattat ccataaagca aatgcctttt caaaagataa aacatttaaa
     6361 agactatgga tcaatagctt agaaaaagat gtaatccgaa gcggttttca aaatttgcaa
     6421 cctggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg
     6481 ttgatcggca tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa
     6541 ggtacatttt cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag
     6601 gaagccatag agaattttaa aaaagaacct tttttcgagg tggaagctag tataaaagta
     6661 aaccaagggt cgtttaaggg cgttctaagc cccacacagc gttttaaaac ccaagaggag
     6721 cttttagctt ttgtttcttc taaacaagct aaaataggca atcaagaggg gataattgct
     6781 gatgttcaaa ccaaagagaa gaaaacgaat agtccgagtt tgttttcttt aagtagtttg
     6841 caatcaaaag tcaatcagct ttataaagcg acagcgagcc aaactttaaa agctatttct
     6901 tttttaataa cttaaaaata aacttaatgt aacagcaagc acagtcaagg tatacacctt
     6961 tgacaaaaaa tagcacattc tctatcgaaa atttttgctt attttttaaa ttattttggg
     7021 aaattttccc aatccctttt tctaactcaa aaaatataat cactcaaaat ttaaaagggc
     7081 gcacttatac atcattttaa aaaattgatg taacgtgcta agttcaaaac aaagggcgca
     7141 cttatacacg attttcaatc ttgtatattt ctaacgaaaa gcgtgcgcca aaaaaccccc
     7201 ttcgtcaatt ttgacagggg gctttttgat gtaaaaattt ctatcgaaat ttaaaaattc
     7261 gcttcactca tgttataaag acttaaaata aaataactct ttaaaatctt ttgctagttg
     7321 ttcttcaata ttttttattc ggtgcatctt ccaagtaaag tataacacac tagacttatt
     7381 tactacgttt cataagtcat taatgcgtgt gctctgcgag gctagttttt gtgcaagcac
     7441 aaaaaatgga ctgaataaat cagtccataa gttcaaaacc aaattcaaaa tcaaaaccac
     7501 aagcaaccaa aaaatgtggt tgttatacgt tcataaattt tatgatcact tacgtgtata
     7561 aaattaaatt cactttcaaa atctaaaaac taaatccaat catctaccct atgaattata
     7621 tcttgaaatt cattcataaa tagtgaagca tggtaaccat cacatacaga atgatgaagt
     7681 tgcagagcaa ctggtatata aattttatta ttctcactat aaaatttacc tatcgtaata
     7741 ataggcaata aaaagctgct attgttacca atatttaaat taaatgaact aaaatcaatc
     7801 caaggaatca ttgaaatcgg tatggtgttt tcaggtatcg gttttttagg aaacatttct
     7861 tctttatctt tatattcaag caagtcattt ttataattat tataaaaaga aatgaagttt
     7921 ttatcagatt cagtccaaat gttagtaaat ttttcagttt gcttattaaa aactgtatac
     7981 aaaggattta acttatccca ataacctaat ttattctcac tattaattcc tgttctaaac
     8041 actttatttt tatttacaac ttccataatt gcataaatta aagagggata aatttcatat
     8101 cctttctttt ttatcatatc tttaaacaaa gtaatatcaa tttctttagt aatgctataa
     8161 gtagtttgct gattaaaata gtgttcaaaa tattcttttc tatcccaatt ttctaattca
     8221 ataatattaa aagtcatata taacttcctc ctaaatttta aatttttata tttaggagga
     8281 ataatcctct gattttttca tacgttatgt cacctcgtaa atattaatta tactgaatta
     8341 gcaattttta tcaaataaaa cttattttac ttccaaaacc taaattcacg ttgccaaaaa
     8401 tcaatctgct tttgcaattg tttttcgttc gcttttaaag tcgatttcat taattccgtt
     8461 aaatcaattg gagatatttc tctaatcaat tttttaaatt tagtcttagt attcttactt
     8521 agctttcccc acatactttc ttcatgcaac aaagtataaa ccatagcttg ctcattaatt
     8581 ttttctaaag tagcccacgc aggtttcaag atgtgtaaat cattaaaaca atcattccag
     8641 taatcaacca tatctctttt taattcaact tctacacgcc ataaatgttc agacacaact
     8701 tcaacatctg cgttatcttt acgttcttgt tttttattat aaattctaat aaatctatca
     8761 ctatcacgga caccaaaata ttttgtttct ggcttgccat tacgaccata aaaaacagtt
     8821 ttcttaactg ctttatcagt cattgcatag taatcgctca aatcatcttc aaaatcaaaa
     8881 gctaagtcta atcttgtaaa accgtcatct tccatgtagt cgataatatt ttgttttaac
     8941 caaatcattt cttcatgtgt gagtttattg ggattaaatt caacacgcat attacgtcta
     9001 tcccaagtat ctgcttttac tttgtcatat tcgatataaa ctttttcttg aagtgcctta
     9061 gctttaaact ttgtttgaag tatatcccaa agtcgtattt gtggctctac actcataaag
     9121 tcagatagct ttttagcatt agttttgttc aaatttccaa cgattgtcat ggcatcaaaa
     9181 cttaatgcgg gttgagattt tcccaaagtt tgaccactta accggctatt acttaaccgg
     9241 ctattagaga cggccggttt ttttagtgag atttctgcag ctaatttttc atcaattttc
     9301 ttcatttttt atcgctccta aaataatttt tggaacgaaa aaaggacgag tggaatcgca
     9361 aaataaattt cgcctgtatc cattcgtcca ttttgttttg tattcaaaat caaaaattga
     9421 tatactaatc atcgaatgaa taacaagtgt ggtttgttat tccgggcgag aacgttggta
     9481 gcgttgctcg tcttttttta tgtctttttt tcgttaaaac tattttaaaa ctaaatatat
     9541 cttaaagcaa ataaattcaa ggggctaaaa gcgcccctta aaaacccctt gcctctgaaa
     9601 agttttgtgt ttcctttccg tcggtttcaa aagctaattt actcaaaaaa atagatcaag
     9661 ggtaaatgca ctcactgcgt tcgcccttga gctattttat ttcgcaaatt ttaacgcatt
     9721 tgccgacgaa aagataaaac acataaaaat atacgcagat atatttaaaa cgtcttagaa
     9781 cgcaaaattt tacgtttaaa agctatgcaa ggactgtatg aagcaaaatt attgagttat
     9841 ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa agcgaatttt
     9901 ggcaaatata gcggtttttt aggacttgat cttgaaatgg ttcaaacaga gcctagaaag
     9961 cgttatgtgg acggtagtaa ggtacaggaa caccacgcca ttatcccaac aaaacaagta
    10021 cctaccgaat ctgcattagc gaaaatggac gatttacaac gaaaaattta tgctttagtc
    10081 gttaaaacga ccgttgccat gtttttacct gattacttgt atgaagaaac caagatacaa
    10141 acgaaagtag ctgatttgct gtttcaatcg atcggaaaga caccaaagca agaagggtgg
    10201 aaaattcttt tcaaacaaca aaccaaagaa gaaaaagagg acgttcaaac gttaccactc
    10261 gttatcattg gcgaacgtgc cgaagttggt gttaagagtg ttgaaaaaga aacgcaaccg
    10321 ccaaaagctt ttacagaggg tacattatta actgctatga aaacggcgaa taaaacggtt
    10381 gatgatgaag aagcaatcaa gattttacaa gaagttgagg ggattggaac agaagcgaca
    10441 agagcaagta ttattgaagc gttaaaacaa aaagaatata tccaagtgat taagaataag
    10501 cttgttgtaa ctgaaaaagg aaaattattg tgccaggcag ttgaaagtca gcacctttta
    10561 acgagtgctg aaatgacggc taaatgggaa acgtatttaa aaaaaatcgg taaaagagaa
    10621 ggcaatcaag agaactttat tacgaatatc aaaaaattca ttgttcattt actggaagct
    10681 gtacctaacg atatagaaaa actaaatttt tctgattacc aggaacagaa agaaaaagaa
    10741 gcagaaaaaa gtattgtagg aaaatgtcct aagtgtggca acaatattgt att
//
LOCUS       DASJPH010000314         7815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.118, whole genome shotgun sequence.
ACCESSION   DASJPH010000314 DASJPH010000000
VERSION     DASJPH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.118"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..577)
                     /locus_tag="U3995_003102"
     CDS             complement(134..577)
                     /locus_tag="U3995_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1798333.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U3995_003103"
     CDS             complement(795..2135)
                     /locus_tag="U3995_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1798334.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U3995_003104"
     CDS             2404..3300
                     /locus_tag="U3995_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1798335.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U3995_003105"
     CDS             complement(3450..4391)
                     /locus_tag="U3995_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1798336.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U3995_003106"
     CDS             4517..5638
                     /locus_tag="U3995_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1798337.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U3995_003107"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U3995_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1798338.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..>7815
                     /locus_tag="U3995_003108"
     CDS             7465..>7815
                     /locus_tag="U3995_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1798339.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLD"
BASE COUNT         2603 a         1471 c         1547 g         2194 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagat
//
LOCUS       DASJPH010000315         4804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.151, whole genome shotgun sequence.
ACCESSION   DASJPH010000315 DASJPH010000000
VERSION     DASJPH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.151"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="U3995_003109"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="U3995_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1798340.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="U3995_003110"
     CDS             765..1919
                     /locus_tag="U3995_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1798341.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="U3995_003111"
     CDS             2134..3102
                     /locus_tag="U3995_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1798342.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="U3995_003112"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="U3995_003112"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1798343.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="U3995_003113"
     CDS             4132..4740
                     /locus_tag="U3995_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1798344.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DASJPH010000316         4250 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.162, whole genome shotgun sequence.
ACCESSION   DASJPH010000316 DASJPH010000000
VERSION     DASJPH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.162"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..398
                     /locus_tag="U3995_003114"
     CDS             <1..398
                     /locus_tag="U3995_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1798345.1"
                     /translation="TSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPNS
                     GQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKTG
                     THHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            885..1349
                     /locus_tag="U3995_003115"
     CDS             885..1349
                     /locus_tag="U3995_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1798346.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            1333..1962
                     /locus_tag="U3995_003116"
     CDS             1333..1962
                     /locus_tag="U3995_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1798347.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            2316..3794
                     /gene="msr(C)"
                     /locus_tag="U3995_003117"
     CDS             2316..3794
                     /gene="msr(C)"
                     /locus_tag="U3995_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1798348.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3841..>4250
                     /locus_tag="U3995_003118"
     CDS             3841..>4250
                     /locus_tag="U3995_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1798349.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLS"
BASE COUNT         1429 a          704 c          875 g         1242 t
ORIGIN      
        1 caacttctcc gatgtatcgt ctgtataacc gtcatacagg agaacattta tatacattga
       61 atgccggaga aaaaaattat ctgccaactg taggctggga atatgaaggg attgcttggc
      121 aagcaccaaa cagcggacaa cctgtctacc gtttatacaa tccttacagt ggagaccatc
      181 attacacaat ggcacaatct gagatcaact ctttaacaaa aatcggttgg cgttatgaag
      241 gtctaagttt ttattctggt ggttccaagc caatttatcg actatttaat ccaaatgaaa
      301 aaactggtac ccatcactat accttgagtg ctaaggaaag agattggcta acaccgatgg
      361 gctggagata tgaaggaata ggattttatg gttattaaaa agaggtagtg aaaaagctgc
      421 cctgcatcaa gtagtaagaa cagccaaaca aaaacagatt ttcatgtttt ttgtttggtt
      481 gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag gttgtgacaa
      541 catcttgtca taactttttt attactgttt aagcatactg atccaatttc taatattcga
      601 cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaatta tctactgcag
      661 aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg ttgtgctttc
      721 tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt ttctattgag
      781 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
      841 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      901 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      961 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
     1021 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
     1081 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
     1141 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg
     1201 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
     1261 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
     1321 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
     1381 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
     1441 gaagcgacga ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa
     1501 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
     1561 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
     1621 atctacaacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
     1681 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
     1741 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1801 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1861 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1921 tctgttcgtt tagcaccaga aaagatcact atgttcgaat aaaaggtttt cagttactat
     1981 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     2041 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     2101 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     2161 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     2221 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     2281 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     2341 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     2401 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     2461 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     2521 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     2581 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     2641 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     2701 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     2761 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2821 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2881 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2941 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     3001 tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa
     3061 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     3121 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     3181 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     3241 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     3301 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     3361 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     3421 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     3481 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     3541 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     3601 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     3661 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     3721 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3781 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3841 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     3901 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     3961 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     4021 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     4081 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     4141 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     4201 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
//
LOCUS       DASJPH010000317         3278 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.177, whole genome shotgun sequence.
ACCESSION   DASJPH010000317 DASJPH010000000
VERSION     DASJPH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.177"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..188
                     /locus_tag="U3995_003119"
     CDS             <1..188
                     /locus_tag="U3995_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1798350.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="U3995_003120"
     CDS             203..1072
                     /locus_tag="U3995_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1798351.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="U3995_003121"
     CDS             1053..1787
                     /locus_tag="U3995_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1798352.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="U3995_003122"
     CDS             1820..2728
                     /locus_tag="U3995_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1798353.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="U3995_003123"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="U3995_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DASJPH010000318         3010 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.183, whole genome shotgun sequence.
ACCESSION   DASJPH010000318 DASJPH010000000
VERSION     DASJPH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.183"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..136
                     /locus_tag="U3995_003124"
                     /pseudo
     CDS             1..136
                     /locus_tag="U3995_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            381..497
                     /locus_tag="U3995_003125"
     CDS             381..497
                     /locus_tag="U3995_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1798354.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            513..2432
                     /gene="tet(M)"
                     /locus_tag="U3995_003126"
     CDS             513..2432
                     /gene="tet(M)"
                     /locus_tag="U3995_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1798355.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2551..2718
                     /locus_tag="U3995_003127"
     CDS             2551..2718
                     /locus_tag="U3995_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1798356.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2778..>3010)
                     /locus_tag="U3995_003128"
     CDS             complement(2778..>3010)
                     /locus_tag="U3995_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1798357.1"
                     /translation="LTNIENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRL
                     QIEKYMDSFTDKELSLMESLASGINEARNIED"
BASE COUNT         1023 a          443 c          602 g          942 t
ORIGIN      
        1 cttttctgtt gtgggcaatc aagaggaaga aagggataaa atctgatttg tggtaaatca
       61 ggcaaaggca acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg
      121 gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc
      181 tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt
      241 atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt
      301 gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgtacccag
      361 tttaagaata cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta
      421 tgcctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg gatttttatg
      481 cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag
      541 ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga
      601 ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac
      661 gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg
      721 tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat
      781 cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc
      841 gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga
      901 ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg
      961 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
     1021 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
     1081 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
     1141 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
     1201 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
     1261 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc
     1321 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
     1381 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     1441 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     1501 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     1561 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     1621 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     1681 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     1741 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     1801 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     1861 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     1921 atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa caaggattgt
     1981 atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg
     2041 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     2101 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     2161 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     2221 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     2281 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     2341 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     2401 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     2461 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     2521 gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga
     2581 ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga
     2641 caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca
     2701 aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta
     2761 tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa
     2821 gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat
     2881 cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga
     2941 agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata
     3001 ttagttaagt
//
LOCUS       DASJPH010000319         2627 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.189, whole genome shotgun sequence.
ACCESSION   DASJPH010000319 DASJPH010000000
VERSION     DASJPH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.189"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..808
                     /locus_tag="U3995_003129"
     CDS             116..808
                     /locus_tag="U3995_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1798358.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1135..1632
                     /gene="dfrG"
                     /locus_tag="U3995_003130"
     CDS             1135..1632
                     /gene="dfrG"
                     /locus_tag="U3995_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1798359.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1704..>2627)
                     /locus_tag="U3995_003131"
     CDS             complement(1704..>2627)
                     /locus_tag="U3995_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1798360.1"
                     /translation="FKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKIT
                     NRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLS
                     DAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSH
                     QFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVN
                     DHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRP
                     FKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          969 a          385 c          472 g          801 t
ORIGIN      
        1 gagtatttga tagtatttat attaaaatta catatgtaat ttaaaaacgc ttgacattgt
       61 gtttgagatc acttactttt agcatgtaaa agtattggat taaggagcta tgcaaatgaa
      121 aaagaaattt attactggtg ggattgtcgt tgcatttcta tgtctaattg gctacacagg
      181 cacgcggtac gcaaatgaaa ttattttgtg gggtggagag acagatattg atacaatcaa
      241 tgagaatctg acacagcttg atcatacatt gacagataaa gagcaagcga ttacagatct
      301 taccgctcga ctagcacaaa aagaacagac attgcaagat tcaaaggcag atgtccaaga
      361 ataccaaaca aaagtaaatg aattagaaaa ccaaaaaaat caatcggcag cagaaaaaca
      421 aaacttagaa agtcaattag tcagcaagga tgcacaacta caaagcaaac aaaatgaaat
      481 caatgtcaaa ctagatgaga tcaaccaaaa aaatgatgaa atcaatcatc tgaatgcaga
      541 ttggacagca agactacaag aagcacaagc aaaactttca gaattacaaa atactgtaaa
      601 tggtttgaat caacaaatcg caacactgac acaagaaaaa acaaatgtag aaactcagtt
      661 aacagataca caaaagaaat tagacgaagc aactgctgaa aactcaagct tgaaacaata
      721 tattcaaaag ttagaaaaag caaaagatga agtggaagat acagctgata aatctcagca
      781 aattgttgaa gaacatttgc gtaaataatc tttcttaaca agtaaaccta aattagtaaa
      841 gaagcaacag taatctatac taagtagttc agttttggag tacaaaatga caaaatgtac
      901 ttcaaaatgg actacttttt tcattttaac ttacatacca aattacatac aaaatgattt
      961 ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata
     1021 atataactta tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt
     1081 tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa
     1141 gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct
     1201 tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata
     1261 ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt
     1321 ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat
     1381 gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat
     1441 aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa
     1501 ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa
     1561 gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac
     1621 ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt
     1681 gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt
     1741 ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa
     1801 gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga
     1861 ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca
     1921 agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca
     1981 tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca
     2041 actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat
     2101 ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt
     2161 aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata
     2221 tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg
     2281 cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc
     2341 gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat
     2401 tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca
     2461 tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga
     2521 taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct
     2581 aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaa
//
LOCUS       DASJPH010000320         2297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.198, whole genome shotgun sequence.
ACCESSION   DASJPH010000320 DASJPH010000000
VERSION     DASJPH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.198"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1028)
                     /locus_tag="U3995_003132"
     CDS             complement(<1..1028)
                     /locus_tag="U3995_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1798361.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREK"
     gene            1581..2129
                     /gene="aac(6')"
                     /locus_tag="U3995_003133"
     CDS             1581..2129
                     /gene="aac(6')"
                     /locus_tag="U3995_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1798362.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          738 a          405 c          450 g          704 t
ORIGIN      
        1 aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta
       61 tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga
      121 agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc
      181 atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt
      241 tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta
      301 actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca
      361 tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga
      421 tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca
      481 cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag
      541 tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt
      601 gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg
      661 acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca
      721 ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct
      781 ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg
      841 taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct
      901 gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg
      961 attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt
     1021 tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc
     1081 gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata
     1141 aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa
     1201 cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc
     1261 ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat
     1321 ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa
     1381 cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt
     1441 ctgtataatt tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
     1501 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
     1561 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
     1621 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
     1681 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
     1741 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
     1801 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
     1861 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
     1921 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
     1981 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
     2041 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
     2101 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
     2161 tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt
     2221 atttccgaag aagctacttt tggaacaccg tgtaccagag gttgtgaaaa agtcgcttag
     2281 tttcgagtag taagaaa
//
LOCUS       DASJPH010000321         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.243, whole genome shotgun sequence.
ACCESSION   DASJPH010000321 DASJPH010000000
VERSION     DASJPH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.243"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..76
                     /locus_tag="U3995_003134"
     CDS             11..76
                     /locus_tag="U3995_003134"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1798363.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="U3995_003135"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="U3995_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1798364.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DASJPH010000322          729 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1369
            SAMN38756148-rid20573773.guided.269, whole genome shotgun sequence.
ACCESSION   DASJPH010000322 DASJPH010000000
VERSION     DASJPH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756148
            Sequence Read Archive: SRR27168095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028248.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028248.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:06:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,088
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 47
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 35 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 9 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756148-rid20573773.guided.269"
                     /isolate="VE1369"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-31"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          239 a          140 c           99 g          251 t
ORIGIN      
        1 gttctataat aattcttgaa ctccatgtct tataatgttc tatcgaagca cccaaatagc
       61 caagttcatc taatctactt gcataaacta cgtcatccgc tctcatagtc ggcatataca
      121 tattaaagag taaaaagata ccccaaatcc ctattagtaa caagatcagt tctttgttat
      181 tcgttatttt tctattatgt ttgttcattt ttatactcct tcttatattt cctaatagag
      241 tataacatag ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa
      301 ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat
      361 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
      421 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
      481 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
      541 tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
      601 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
      661 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
      721 attgatctg
//
